CN114788796B

CN114788796B - Whitening and antibacterial cosmetic containing Fuding white tea extract and preparation method thereof

Info

Publication number: CN114788796B
Application number: CN202210240314.3A
Authority: CN
Inventors: 刘雨晴; 范毅; 王韬; 李建; 张华南; 刘冠华; 景炳年; 魏磊; 谢晓阳; 朱杰; 陈飞; 崔炜
Original assignee: Henan Napu Biotechnology Co ltd; Henan Academy of Sciences
Current assignee: Henan Napu Biotechnology Co ltd; Henan Academy of Sciences
Priority date: 2022-03-10
Filing date: 2022-03-10
Publication date: 2023-08-25
Anticipated expiration: 2042-03-10
Also published as: CN114788796A

Abstract

The invention discloses a whitening and antibacterial cosmetic containing Fuding white tea extract and a preparation method thereof. The whitening and antibacterial cosmetic is prepared by taking Fuding white tea extract, ligusticum wallichii extract, sea buckthorn polysaccharide extract and pricklyash seed antibacterial peptide as active ingredients and adding other auxiliary agents including 1, 3-butanediol, sodium hyaluronate, polyglycerol fatty acid ester, squalane, cetylstearyl alcohol, tetraisopalmitate ascorbate, stearyl alcohol, sodium isostearyl lactoyl lactate and the balance deionized water; in the preparation process, the water phase material components are added into a water phase pot for heating and mixing uniformly to obtain a water phase material; adding the oil phase material components into an oil phase pot, heating and uniformly mixing to obtain an oil phase material; and finally homogenizing the water phase material and the oil phase material in an emulsifying pot to obtain the whitening antibacterial cosmetic. The product of the invention is verified to have good whitening efficacy, safety and warmth and can be used for a long time.

Description

Whitening and antibacterial cosmetic containing Fuding white tea extract and preparation method thereof

1. Technical field:

the invention relates to the technical field of raw materials and preparation of cosmetics, in particular to a whitening and antibacterial cosmetic containing Fuding white tea extract and a preparation method thereof.

2. The background technology is as follows:

whitening is a fashion topic that our asians never fade, and in addition, in western countries, whitening products are applied to the prevention and treatment of irregular hyperpigmentation, such as age spots, freckles, etc. Therefore, the whitening is always the main melody of the cosmetic market, and the whitening industry has a great development space.

However, the traditional whitening active ingredients have the defects of mercury compounds, hormone-like substances, hydroquinone and the like, wherein the mercury compounds, the hormone-like substances, the hydroquinone and the like are easy to deposit in a human body, have high cytotoxicity, strong skin irritation, high sensitization, are easy to cause adverse reactions and the like, and are forbidden in the sanitary regulations of a plurality of countries. The natural active ingredients derived from the traditional Chinese medicine are taken as the additive of the whitening skin care product, and are favored by people because of small toxic and side effects, high safety, mildness and no irritation to skin. The single Chinese herbal medicine contains effective whitening active ingredients which are insufficient to meet the requirements of skin on various nutritional ingredients, and the synergistic effect of compounding the traditional Chinese medicines can often obtain the advantages of better whitening effect, mild effect, promotion of skin metabolism and the like compared with the single extract. The research and development of the whitening traditional Chinese medicine compound has wide application prospect and development value.

The amount and distribution of melanin determines the color of the skin. After melanin is formed in melanocytes of basal skin layers, the life cycle of melanin also includes a series of complex processes of migration, metabolism, degradation, and excretion, and has a close relationship with keratinocytes and fibroblasts. Therefore, the tyrosinase activity is inhibited, and the proliferation of skin melanocytes is inhibited, so that the melanin generation is reduced, and the effect of whitening the skin is achieved. The efficacy evaluation of the whitening cosmetics at the present stage mainly comprises three systems: non-cellular Tyrosinase (TYR) testing system, cellular testing system, and reconstituted skin model testing system. The cell test system can rapidly and effectively evaluate the whitening effect of the active substances, and is a common method for researching the whitening effect of the active substances.

Fuding white tea mainly comes from Fuding, politics, jianyang and Songxi, and has the advantages of unique manufacturing process, no stir-frying, no kneading, natural and simple process, and natural achievement after two processes of withering and drying of fresh leaves. Modern researches show that the white tea has the highest flavone content and the lowest free radical content, and has good oxidation resistance and radiation resistance. Meanwhile, the Chinese medicinal composition has the effects of treating and relieving partial diseases and adverse conditions, and mainly has the effects of improving eyesight, refreshing, resisting oxidation, delaying aging, regulating intestinal flora, improving immunity, strengthening teeth, reducing blood fat, lowering blood pressure, losing weight, preventing cardiovascular and cerebrovascular diseases, promoting urination, relieving summer heat, protecting against radiation, preventing allergy and the like. Fuding white tea also has remarkable effects of beautifying, resisting aging and resisting photoaging of skin. The invention patent application CN201611116033.8 discloses a white tea extract and a preparation method and application thereof, and relates to a white tea extract with multiple functions of moisturizing, whitening, moistening, resisting aging and the like.

Ligusticum wallichii is a dried rhizome of a plant Ligusticum chuanxiong Hort of Ligusticum of Umbelliferae, mainly produced in Sichuan, guizhou, yunnan, hubei, guangxi and other places. It has warm nature and pungent taste, and has effects of promoting blood circulation, activating qi-flowing, dispelling pathogenic wind, removing speckle, and caring skin. The traditional Chinese medicine considers that the ligusticum wallichii has the whitening effect, the traditional record of using the ligusticum wallichii for whitening is recorded, and the literature records: the recorded beauty prescription 363 of the external table secret recipe, 274 of which is an external beauty prescription and 89 of which is an internal beauty prescription. The biological active components of the ligusticum wallichii are complex and comprise organic phenolic acids, alkaloids, lactones, phthalides, polysaccharides and the like according to chemical structures.

The 50% ethanol water extract of Ligusticum wallichii has high antioxidation and mushroom tyrosinase inhibition effects. The ligusticum wallichii phenolic acid is the most important active ingredient of ligusticum wallichii, and has various pharmacological activities of resisting oxidation, scavenging free radicals, whitening, resisting platelet aggregation, resisting thrombus, preventing and treating coronary heart disease and the like.

The sea buckthorn is deciduous shrub or small arbor, belongs to elaeagnus pungens, can prevent wind and fix sand, is rich in polysaccharide, flavone, organic acid and other components, and has various activities, such as antibacterial, antioxidant and anti-aging effects, in different parts, especially berries, of the sea buckthorn, which are regarded as traditional medicines for Xinjiang, inner Mongolia and Tibet. The Hippophae rhamnoides polysaccharide has the effects of resisting tumor and preventing cardiovascular and cerebrovascular diseases in medicine. The polysaccharide is a natural polymer found in animal cell membranes, higher plants and microbial cell walls, has no toxic or side effect and wide biological activity, is a natural recurring unit (monosaccharide or disaccharide) polymeric carbohydrate, is connected together through glycosidic bonds, is an essential biological macromolecule in life activities, has the functions of immunoregulation, anti-tumor, antioxidation and the like, and is widely studied in the fields of functions and medicine.

The plant antibacterial peptide is a natural active substance with broad-spectrum antibacterial activity, fungi, viruses and other biological activities, and microbial pathogenic bacteria have drug resistance to common antibiotics. Therefore, the search for the development of novel antibiotics from plant antibacterial peptides to control these pathogenic microorganisms has important theoretical and practical significance. In addition, the antibacterial peptide also has a certain biological activity of promoting the healing of organism tissues and regulating in-vivo immunity.

3. The invention comprises the following steps:

the invention aims to solve the technical problems that: in order to overcome the defects in the prior art, the invention provides a whitening and antibacterial cosmetic containing Fuding white tea extract and a preparation method thereof.

In order to solve the problems, the invention adopts the following technical scheme:

the invention provides a whitening and antibacterial cosmetic composition containing Fuding white tea extract, which consists of raw materials of Fuding white tea extract, ligusticum wallichii extract, sea buckthorn polysaccharide extract and pricklyash peel seed antibacterial peptide, wherein the mass ratio of the four is 42-48: 40-46: 7.5 to 11.5:0.25 to 0.5.

According to the whitening and antibacterial cosmetic composition containing the Fuding white tea extract, the preparation method of the Fuding white tea extract comprises the following steps:

The Fuding white tea is placed in an extraction tank, water is added, and the mass volume ratio of the Fuding white tea to the water is 1g: 30-50 mL, and then heating to boiling condition for extraction for 10-20 min; extracting for 2-3 times according to the method, combining the obtained extracting solutions, filtering and concentrating the extracting solutions to obtain a pasty crude extract, thus obtaining the Fuding white tea extract.

According to the whitening and antibacterial cosmetic composition containing the Fuding white tea extract, the preparation method of the ligusticum wallichii extract comprises the following steps of:

pulverizing rhizoma Ligustici Chuanxiong root to below 20 mesh, and ultrasonic extracting with 50-90% ethanol at 60-75deg.C for 50-70 min; ultrasonic extracting for 2-3 times under the same condition, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract, thus obtaining the ligusticum chuanxiong hort extract.

According to the whitening and antibacterial cosmetic composition containing the Fuding white tea extract, the preparation method of the sea buckthorn polysaccharide extract comprises the following steps:

crushing dry sea buckthorn fruits, sieving with a 40-mesh sieve, and then adding petroleum ether with the volume of 10-15 times for reflux extraction for 2-3 times, wherein the extraction time is 40-80 min each time; mixing the extracts, removing fat-soluble substances, and performing suction filtration to obtain filter residues for later use;

The residue obtained was purified according to a ratio of 1g: distilled water is added into the feed liquid with the ratio of 20-30 mL, ultrasonic auxiliary extraction is carried out at the temperature of 65-75 ℃, the obtained extracting solution is concentrated to 1g/mL (namely 1g of sea buckthorn raw material is contained in 1mL of the obtained concentrate); adding 3-5 times volume of absolute ethyl alcohol into the obtained concentrate, cooling to 4 ℃ and standing for 10-14 h to generate precipitate, then carrying out precipitation centrifugation, removing protein (removing protein for 5 times) from the solution obtained after centrifugation, drying the substance obtained after protein removal in an oven at the drying temperature of 35-45 ℃ to obtain the sea buckthorn crude polysaccharide extract.

According to the whitening and antibacterial cosmetic composition containing the Fuding white tea extract, the preparation method of the pricklyash seed antibacterial peptide comprises the following steps of:

a. pulverizing semen Zanthoxyli to below 30 mesh to obtain semen Zanthoxyli powder as raw material;

b. degreasing the obtained pricklyash seed powder by petroleum ether to obtain degreased pricklyash seed powder;

c. adding 10 times of NaOH solution with concentration of 0.5% into the defatted pricklyash seed powder, and stirring for 30min; centrifuging at 8000 r.min for 20min, and collecting supernatant; adding 10 times of NaOH solution with the concentration of 0.5% into the centrifuge tube again, and repeating the above steps; combining the supernatants obtained by 2 times of centrifugation, and freeze-drying after combining to obtain the pricklyash seed protein;

d. Weighing the obtained pricklyash seed protein, dissolving the pricklyash seed protein in deionized water, enabling the mass concentration of the pricklyash seed protein to be 30-50 mg/mL, preheating the pricklyash seed protein in a constant-temperature water bath kettle with the temperature of 32-50 ℃, and then adding acid protease for enzymolysis; continuously stirring in the enzymolysis process, and dropwise adding HCl or NaOH to maintain the pH value of the solution to be 2.1-4.0; taking out the obtained product after enzymolysis, putting the obtained product into a water bath kettle at 95 ℃ for enzyme deactivation for 15min, cooling, centrifuging for 10min at the temperature of 4 ℃ and under the condition of 8000r/min, taking supernatant to adjust the pH value to be neutral, and then carrying out vacuum freeze drying to obtain freeze-dried powder, namely the pricklyash seed antibacterial peptide, and preserving for later use;

the enzymolysis conditions are as follows: the mass ratio of the acid protease to the pricklyash seed protein is 0.9-3: 100. the pH value is 2.1-4.0, and the enzymolysis time is 3.2-5.0 h.

In addition, the whitening and antibacterial cosmetic containing the Fuding white tea extract is provided, and the weight percentage of the whitening and antibacterial cosmetic composition in the cosmetic raw material composition is 1-10%.

According to the whitening and antibacterial cosmetic containing the Fuding white tea extract, the raw materials of the whitening and antibacterial cosmetic comprise the following components in percentage by mass: 0.44 to 1.76 percent of Fuding white tea extract, 0.115 to 0.46 percent of sea buckthorn polysaccharide extract, 0.44 to 1.76 percent of szechuan lovage rhizome extract, 0.005 to 0.02 percent of pricklyash peel seed antibacterial peptide, 1 to 5 percent of 1, 3-butanediol, 1 to 1.2 percent of sodium hyaluronate, 1 to 1.3 percent of polyglycerol fatty acid ester, 1 to 1.8 percent of squalane, 1 to 1.8 percent of cetostearyl alcohol, 0.04 to 0.1 percent of tetraisopalmitate of ascorbic acid, 1 to 1.8 percent of stearyl alcohol and 1 to 1.8 percent of isostearyl lactoyl sodium lactate, and the balance of deionized water, wherein the sum of the percentage of the components is 100 percent.

According to the whitening and antibacterial cosmetic containing the Fuding white tea extract, the raw materials of the whitening and antibacterial cosmetic comprise the following components: 0.88% of Fuding white tea extract, 0.23% of sea buckthorn polysaccharide extract, 0.88% of ligusticum wallichii extract, 0.01% of pepper seed antibacterial peptide, 3% of 1, 3-butanediol, 1.2% of sodium hyaluronate, 1.1% of polyglycerol fatty acid ester, 1.5% of squalane, 1.2% of cetostearyl alcohol, 0.05% of ascorbyl tetraisopalmitate, 1.2% of stearyl alcohol and 1.2% of isostearyl lactoyl sodium lactate, and the balance of deionized water, wherein the sum of the percentages of the components is 100%.

The invention provides a preparation method of a whitening antibacterial cosmetic containing Fuding white tea extract, which comprises the following steps:

1) Preparing various raw materials according to the raw material composition of the whitening antibacterial cosmetic, firstly adding Fuding white tea extract, sea buckthorn polysaccharide extract, 1, 3-butanediol, sodium hyaluronate and deionized water into a water phase pot, stirring, heating to 40-50 ℃ after uniformly mixing, and obtaining a water phase material;

2) Then sequentially adding polyglycerol fatty acid ester, rhizoma Ligustici Chuanxiong extract, squalane, fructus Zanthoxyli seed antibacterial peptide, cetostearyl alcohol, ascorbyl tetraisopalmitate, stearyl alcohol and isostearyl lactoyl sodium lactate into an oil phase pot, heating to 40-50deg.C, stirring and mixing to obtain oil phase material;

3) Pumping the water phase material into an emulsifying pot, stirring and homogenizing, pumping the oil phase material, and homogenizing for 15-25 minutes at 40-50 ℃ to obtain the whitening antibacterial skin cream containing Fuding white tea extract.

The invention has the positive beneficial effects that:

1. according to the technical scheme, the composition with the whitening and antibacterial effects, which is prepared from the Fuding white tea extract, the ligusticum chuanxiong extract, the sea buckthorn polysaccharide extract and the plant antibacterial peptide serving as active raw materials, has good whitening and antibacterial effects through B16 cell proliferation, melanin synthesis, tyrosinase activity and antibacterial activity tests.

2. The whitening antibacterial skin cream prepared by the technical scheme of the invention is proved to be safe and mild through performance test, capability of improving skin brightness, freckle removal, inflammation diminishing, greasy feeling, moisturizing effect and other whitening effect satisfaction test and mildness test, and can be used for a long time; the preparation method is simple to operate and suitable for industrial production.

4. The specific embodiment is as follows:

the invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.

Example 1:

the invention relates to a whitening and antibacterial cosmetic composition containing Fuding white tea extract, which consists of raw materials of Fuding white tea extract, ligusticum wallichii extract, sea buckthorn polysaccharide extract and pricklyash peel seed antibacterial peptide, wherein the mass ratio of the four is 42:46:10.0:0.5.

The preparation method of the Fuding white tea extract comprises the following steps: the Fuding white tea is placed in an extraction tank, water is added, and the mass volume ratio of the Fuding white tea to the water is 1g:50mL, and then heating to boiling condition for extraction for 10min; extracting for 2 times according to the method, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract, thus obtaining the Fuding white tea extract.

The preparation method of the ligusticum wallichii extract comprises the following steps: pulverizing rhizoma Ligustici Chuanxiong root to below 20 mesh, and performing ultrasonic extraction with 80% ethanol at 65deg.C for 60min at a feed-liquid ratio of 1:20 g/mL; ultrasonic extracting for 2 times under the same condition, mixing the obtained extracting solutions, filtering, concentrating to obtain pasty crude extract, and obtaining the ligusticum chuanxiong hort extract.

The preparation method of the sea buckthorn polysaccharide extract comprises the following steps: pulverizing dry fructus Hippophae, sieving with 40 mesh sieve, and reflux extracting with 10 times of petroleum ether for 2 times for 60min each time; mixing the extracts, removing fat-soluble substances, and performing suction filtration to obtain filter residues for later use;

the residue obtained was purified according to a ratio of 1g: adding distilled water into 20mL of feed liquid ratio, performing ultrasonic-assisted extraction at 70 ℃, concentrating the obtained extract to 1g/mL (namely 1g of sea buckthorn raw material in 1mL of concentrate); adding 3 times volume of absolute ethyl alcohol into the obtained concentrate, cooling to 4 ℃ and standing for 12 hours to generate precipitate, then carrying out precipitation centrifugation, carrying out protein removal on the solution obtained after centrifugation for 5 times, drying the substance obtained after protein removal in an oven at the drying temperature of 40 ℃, and drying to obtain the sea buckthorn crude polysaccharide extract.

The preparation method of the pricklyash seed antibacterial peptide comprises the following steps:

d. weighing the obtained pricklyash seed protein, dissolving in deionized water to make the mass concentration of the pricklyash seed protein be 50mg/mL, preheating in a constant-temperature water bath kettle with the temperature of 32 ℃, and then adding acid protease for enzymolysis; continuously stirring in the enzymolysis process, and dropwise adding HCl or NaOH to maintain the pH value of the solution to be 2.1; taking out the obtained product after enzymolysis, putting the obtained product into a water bath kettle at 95 ℃ for enzyme deactivation for 15min, cooling, centrifuging for 10min at the temperature of 4 ℃ and under the condition of 8000r/min, taking supernatant to adjust the pH value to be neutral, and then carrying out vacuum freeze drying to obtain freeze-dried powder, namely the pricklyash seed antibacterial peptide, and preserving for later use;

The enzymolysis conditions are as follows: the mass ratio of the acid protease to the pricklyash seed protein is 0.9: 100. the pH value is 2.1, and the enzymolysis time is 3.2h.

Example 2:

the invention relates to a whitening and antibacterial cosmetic composition containing Fuding white tea extract, which consists of raw materials of Fuding white tea extract, ligusticum wallichii extract, sea buckthorn polysaccharide extract and pricklyash peel seed antibacterial peptide, wherein the mass ratio of the four is 48:40:7.5:0.25.

the preparation method of the Fuding white tea extract comprises the following steps: the Fuding white tea is placed in an extraction tank, water is added, and the mass volume ratio of the Fuding white tea to the water is 1g:30mL, and then heating to boiling condition for extraction for 10min; extracting for 2 times according to the method, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract, thus obtaining the Fuding white tea extract.

The preparation method of the ligusticum wallichii extract comprises the following steps: pulverizing rhizoma Ligustici Chuanxiong root to below 20 mesh, performing ultrasonic extraction with 80% ethanol at 65deg.C for 60min at a feed-liquid ratio of 1:25 g/mL; ultrasonic extracting for 2 times under the same condition, mixing the obtained extracting solutions, filtering, concentrating to obtain pasty crude extract, and obtaining the ligusticum chuanxiong hort extract.

The preparation method of the sea buckthorn polysaccharide extract comprises the following steps: pulverizing dry fructus Hippophae, sieving with 40 mesh sieve, and reflux extracting with petroleum ether 12 times of volume for 2 times for 70min each time; mixing the extracts, removing fat-soluble substances, and performing suction filtration to obtain filter residues for later use;

d. Weighing the obtained pricklyash seed protein, dissolving in deionized water to make the mass concentration of the pricklyash seed protein be 30mg/mL, preheating in a constant-temperature water bath kettle with the temperature of 45.5 ℃, and then adding acid protease for enzymolysis; continuously stirring in the enzymolysis process, and dropwise adding HCl or NaOH to maintain the pH value of the solution to be 4.0; taking out the obtained product after enzymolysis, putting the obtained product into a water bath kettle at 95 ℃ for enzyme deactivation for 15min, cooling, centrifuging for 10min at the temperature of 4 ℃ and under the condition of 8000r/min, taking supernatant to adjust the pH value to be neutral, and then carrying out vacuum freeze drying to obtain freeze-dried powder, namely the pricklyash seed antibacterial peptide, and preserving for later use;

the enzymolysis conditions are as follows: the mass ratio of the acid protease to the pricklyash seed protein is 3.0: 100. the pH value is 4.0, and the enzymolysis time is 4.8 hours.

Example 3:

the invention relates to a whitening and antibacterial cosmetic composition containing Fuding white tea extract, which consists of raw materials of Fuding white tea extract, ligusticum wallichii extract, sea buckthorn polysaccharide extract and pricklyash peel seed antibacterial peptide, wherein the mass ratio of the four is 44:44:11.5:0.5.

the preparation method of the Fuding white tea extract comprises the following steps: the Fuding white tea is placed in an extraction tank, water is added, and the mass volume ratio of the Fuding white tea to the water is 1g:35mL, then heating to boiling condition and extracting for 10min; extracting for 2 times according to the method, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract, thus obtaining the Fuding white tea extract.

The preparation method of the ligusticum wallichii extract comprises the following steps: pulverizing rhizoma Ligustici Chuanxiong root to below 20 mesh, and performing ultrasonic extraction with 80% ethanol at 65deg.C for 60min at a feed-liquid ratio of 1:18 g/mL; ultrasonic extracting for 2 times under the same condition, mixing the obtained extracting solutions, and concentrating to obtain the rhizoma Ligustici Chuanxiong extract.

The preparation method of the sea buckthorn polysaccharide extract comprises the following steps: pulverizing dry fructus Hippophae, sieving with 40 mesh sieve, and reflux extracting with 15 times of petroleum ether for 2 times for 60min each time; mixing the extracts, removing fat-soluble substances, and performing suction filtration to obtain filter residues for later use;

the residue obtained was purified according to a ratio of 1g: adding distilled water into 25mL of feed liquid ratio, performing ultrasonic-assisted extraction at 65 ℃, concentrating the obtained extract to 1g/mL (namely 1g of sea buckthorn raw material in 1mL of concentrate); adding 3 times volume of absolute ethyl alcohol into the obtained concentrate, cooling to 4 ℃ and standing for 12 hours to generate precipitate, then carrying out precipitation centrifugation, carrying out protein removal on the solution obtained after centrifugation for 5 times, drying the substance obtained after protein removal in an oven at the drying temperature of 40 ℃, and drying to obtain the sea buckthorn crude polysaccharide extract.

d. weighing the obtained pricklyash seed protein, dissolving in deionized water to make the mass concentration of the pricklyash seed protein 40mg/mL, preheating in a constant-temperature water bath kettle at 42.1 ℃, and then adding acid protease for enzymolysis; continuously stirring in the enzymolysis process, and dropwise adding HCl or NaOH to maintain the pH value of the solution to be 3; taking out the obtained product after enzymolysis, putting the obtained product into a water bath kettle at 95 ℃ for enzyme deactivation for 15min, cooling, centrifuging for 10min at the temperature of 4 ℃ and under the condition of 8000r/min, taking supernatant to adjust the pH value to be neutral, and then carrying out vacuum freeze drying to obtain freeze-dried powder, namely the pricklyash seed antibacterial peptide, and preserving for later use;

The enzymolysis conditions are as follows: the mass ratio of the acid protease to the pricklyash seed protein is 2: 100. the pH value is 3, and the enzymolysis time is 3.5h.

Example 4:

the invention relates to a whitening and antibacterial cosmetic composition containing Fuding white tea extract, which consists of raw materials of Fuding white tea extract, ligusticum wallichii extract, sea buckthorn polysaccharide extract and pricklyash peel seed antibacterial peptide, wherein the mass ratio of the four is 46:42:9.0:0.35.

the preparation method of the Fuding white tea extract comprises the following steps: the Fuding white tea is placed in an extraction tank, water is added, and the mass volume ratio of the Fuding white tea to the water is 1g:40mL, and then heating to boiling condition for extraction for 10min; extracting for 2 times according to the method, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract, and obtaining the Fuding white tea extract.

The preparation method of the ligusticum wallichii extract comprises the following steps: pulverizing rhizoma Ligustici Chuanxiong root to below 20 mesh, performing ultrasonic extraction with 80% ethanol at 65deg.C for 60min at a feed-liquid ratio of 1:15 g/mL; ultrasonic extracting for 2 times under the same condition, mixing the obtained extracting solutions, filtering, concentrating to obtain pasty crude extract, and obtaining the ligusticum chuanxiong hort extract.

the residue obtained was purified according to a ratio of 1g: adding distilled water into 30mL of feed liquid ratio, performing ultrasonic-assisted extraction at 70 ℃, concentrating the obtained extract to 1g/mL (namely 1g of sea buckthorn raw material in 1mL of concentrate); adding 3 times volume of absolute ethyl alcohol into the obtained concentrate, cooling to 4 ℃ and standing for 12 hours to generate precipitate, then carrying out precipitation centrifugation, carrying out protein removal on the solution obtained after centrifugation for 5 times, drying the substance obtained after protein removal in an oven at the drying temperature of 40 ℃, and drying to obtain the sea buckthorn crude polysaccharide extract.

d. Weighing the obtained pricklyash seed protein, dissolving in deionized water to make the mass concentration of the pricklyash seed protein be 35mg/mL, preheating in a constant-temperature water bath kettle with the temperature of 36.1 ℃, and then adding acid protease for enzymolysis; continuously stirring in the enzymolysis process, and dropwise adding HCl or NaOH to maintain the pH value of the solution to be 3.2; taking out the obtained product after enzymolysis, putting the obtained product into a water bath kettle at 95 ℃ for enzyme deactivation for 15min, cooling, centrifuging for 10min at the temperature of 4 ℃ and under the condition of 8000r/min, taking supernatant to adjust the pH value to be neutral, and then carrying out vacuum freeze drying to obtain freeze-dried powder, namely the pricklyash seed antibacterial peptide, and preserving for later use;

the enzymolysis conditions are as follows: the mass ratio of the acid protease to the pricklyash seed protein is 1.5: 100. the pH value is 3.2, and the enzymolysis time is 4 hours.

Example 5:

the invention relates to a whitening and antibacterial cosmetic composition containing Fuding white tea extract, which consists of raw materials of Fuding white tea extract, ligusticum wallichii extract, sea buckthorn polysaccharide extract and pricklyash peel seed antibacterial peptide, wherein the mass ratio of the four is 45:43:8.5:0.45.

the preparation method of the Fuding white tea extract comprises the following steps: the Fuding white tea is placed in an extraction tank, water is added, and the mass volume ratio of the Fuding white tea to the water is 1g:45mL, and then heating to boiling condition for extraction for 10min; extracting for 2 times according to the method, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract, thus obtaining the Fuding white tea extract.

The preparation method of the ligusticum wallichii extract comprises the following steps: pulverizing rhizoma Ligustici Chuanxiong root to below 20 mesh, and performing ultrasonic extraction with 80% ethanol at 65deg.C for 60min at a feed-liquid ratio of 1:22 g/mL; ultrasonic extracting for 2 times under the same condition, mixing the obtained extracting solutions, filtering, concentrating to obtain pasty crude extract, and obtaining the ligusticum chuanxiong hort extract.

the residue obtained was purified according to a ratio of 1g: adding distilled water into 22mL of feed liquid ratio, performing ultrasonic-assisted extraction at 70 ℃, concentrating the obtained extract to 1g/mL (namely 1g of sea buckthorn raw material in 1mL of concentrate); adding 3 times volume of absolute ethyl alcohol into the obtained concentrate, cooling to 4 ℃ and standing for 12 hours to generate precipitate, then carrying out precipitation centrifugation, carrying out protein removal on the solution obtained after centrifugation for 5 times, drying the substance obtained after protein removal in an oven at the drying temperature of 40 ℃, and drying to obtain the sea buckthorn crude polysaccharide extract.

d. weighing the obtained pricklyash seed protein, dissolving in deionized water to make the mass concentration of the pricklyash seed protein be 38mg/mL, preheating in a constant-temperature water bath kettle with the temperature of 33 ℃, and then adding acid protease for enzymolysis; continuously stirring in the enzymolysis process, and dropwise adding HCl or NaOH to maintain the pH value of the solution to be 2.8; taking out the obtained product after enzymolysis, putting the obtained product into a water bath kettle at 95 ℃ for enzyme deactivation for 15min, cooling, centrifuging for 10min at the temperature of 4 ℃ and under the condition of 8000r/min, taking supernatant to adjust the pH value to be neutral, and then carrying out vacuum freeze drying to obtain freeze-dried powder, namely the pricklyash seed antibacterial peptide, and preserving for later use;

The enzymolysis conditions are as follows: the mass ratio of the acid protease to the pricklyash seed protein is 1.9: 100. the pH value is 2.8, and the enzymolysis time is 3.9h.

The extract compositions prepared in examples 1 to 5 of the present invention were used for experimental detection, and the specific detection process is as follows:

the whitening antibacterial cosmetic composition obtained in the embodiments 1 to 5 of the present invention is pretreated before the following experiments, and the pretreatment method is as follows:

dissolving the samples to be tested in the examples 1-5 with 5%o DMSO (v/v), preparing a solution with the concentration of 1600.0 mug/mL by distilled water, filtering by a 0.22 mu m filter membrane, and filtering to obtain mother liquor in the examples 1-5; the mother solution is diluted by liquid culture medium in turn, namely the concentration is 800.0, 400.0, 200.0 and 100.0 mug/mL in turn.

1. B16 cell activity assay:

measurement protocol: taking B16 cells growing in logarithmic phase according to 3×10 ⁴ The concentration of each mL is inoculated in a 96-well plate, and 100 mu L of culture solution is filled in each well; 37 ℃ and 5% CO ₂ Culturing in the environment for 24h; the supernatant stock culture was discarded, 100. Mu.L of complete culture solution containing samples to be tested of examples 1 to 5 (100. Mu.g/mL, 200. Mu.g/mL, 400. Mu.g/mL, 800. Mu.g/mL) at different concentrations was added to each well, the control group was set to the same volume of culture solution without the samples to be tested, and the blank wells were not inoculated with cells and only 100. Mu.L of medium was added, each group was set to 3 replicates. The CCK8 method was used to determine cell activity. The cells were cultured at 37℃with 5% CO ₂ The culture was continued for 24 hours, 48 hours, 72 hours, 10. Mu. LCCK-8 solution was added to each well, after 4 hours of culture, the absorbance at 450nm was measured, and the cell viability was measured and calculated using a blank Kong Diaoling. The cell viability was calculated as follows:

cell viability/% = sample absorbance/control absorbance 100

Test results: the cell activity after the whitening antibacterial composition of examples 1 to 5 of the present invention acted on the B16 cells is shown in table 1. The concentration and treatment time can influence the inhibition effect of the sample to be tested on the proliferation of B16 cells. The low-concentration whitening antibacterial composition of the examples 1-5 has no obvious inhibition effect on the activity of B16 cells, and the activity of the B16 cells is not obviously reduced within the treatment time of 72h at the maximum when the concentration of the whitening antibacterial composition of the examples 1-5 is 100 and 200 mu g/mL. 200 μg/mL of the mixtures of examples 1-5 all significantly inhibited B16 cell activity, at which concentration the viability of B16 cells was as low as 90.36% when treated for 72 h; the sample to be tested with the concentration of 400 mug/mL shows extremely obvious difference, and the cell viability is as low as 82.23% after 72 hours. Arbutin (positive control) at a concentration of 100 μg/mL also significantly inhibited the activity of B16 cells, and after 72h treatment, the cell viability was reduced to 78.84%.

TABLE 1 influence of various samples to be tested on B16 cell viability

Note that: p <0.05, < p <0.01 compared to control group.

2. Measurement of intracellular melanin content:

measurement protocol: the melanin content of B16 cells was determined using NaOH lysis. Taking B16 cells growing in logarithmic phase, adjusting cell concentration, inoculating into 6-well plate (3×10) ⁴ And/or holes). 37 ℃ and 5% CO ₂ Culturing in the environment for 24h. The culture broth was aspirated and samples to be tested of examples 1-5 were added at different concentrations, and treated as in B16 cell activity assays, with 1mL added per well. The cells were cultured at 37℃with 5% CO ₂ The culture was continued for 24h, 48h, 72h in the environment, after which the cells were rinsed 3 times with PBS buffer. Then 2mL of NaOH solution with the concentration of 0.2mol/L is added, and the mixture is water-bath for 1h at 80 ℃ to thoroughly dissolve the cell mass. The absorbance at 492nm was measured and the relative melanin content was calculated as follows:

melanin relative content/% = sample absorbance/control absorbance x 100

Measurement results: the effect on melanin content of B16 cells after their action is shown in Table 2 for the compositions prepared in examples 1-5 of the present invention. Based on the effect of the compositions of examples 1-5 on B16 cell viability, the treatment concentrations were selected to be 100 μg/mL and 200 μg/mL for the melanin content determination, at which the activity of B16 cells was not significantly inhibited. But has obvious inhibition effect on the melanin synthesis of B16 cells, and the inhibition effect is gradually enhanced along with the increase of the concentration, and the B16 cells are treated for 72 hours with 200 mug/mL, so that the melanin content in the cells is 74.35 percent, namely, the inhibition rate on the melanin generation amount reaches 25.65 percent. The whitening antibacterial composition containing Fuding white tea extract prepared by the invention is a potential melanin synthesis inhibitor.

TABLE 2 Effect of various test samples on B16 cell melanin synthesis

Note that: values are the average of 3 replicates ± standard error, different lower case letters in the same column represent 5% difference significance levels, the following.

3. Effect on B16 cell tyrosinase activity:

measurement protocol: cell culture and treatment methods are used for cell activity determination. The cells treated in examples 1-5 were further cultured for 24 hours, 48 hours, 72 hours, the culture medium was aspirated and washed 3 times with PBS buffer. mu.L of cell lysate 1% TritonX-100 was added, frozen at low temperature for 1 hour, and thawed at room temperature after removal. Incubate at 37℃and add 10. Mu.L of 1% strength L-DOPA solution and react for 1h. The absorbance at 492nm was measured and the tyrosinase relative activity was calculated as follows:

relative activity of the ku-amidase/% = (1-sample group absorbance/control group absorbance) ×100

The B16 cell tyrosinase activities of the whitening antibacterial compositions of the examples 1-5 are shown in Table 3, and compared with the control group, the whitening antibacterial compositions of the examples 1-5 have remarkable inhibition effect on the B16 cell tyrosinase activities. With the increase of the concentration and the increase of the treatment time of the whitening antibacterial composition in examples 1-5, the tyrosinase activity of B16 cells is gradually reduced, and the dosage dependence is obviously different from that of a control group (p < 0.05). Within the experimental setting range, the inhibition rate of the whitening antibacterial composition of the embodiment 1-5 on the tyrosinase activity of B16 cells is 10.76-46.64%.

TABLE 3 Effect of the whitening antibacterial compositions of examples 1-5 of the present invention on B16 cell tyrosinase activity

Melanin in human skin is produced by melanocytes, can be transferred into keratinocytes through the dendritic structure of the cells, and can be rearranged. Therefore, with the differentiation of keratinocytes, melanin can migrate upward and eventually migrate to the stratum corneum of the skin, forming pigmentation on the skin surface. Currently, the synthesis of melanin has been explored by scholars. First, tyrosine in melanocytes reacts with dopa under the catalytic action of Tyrosinase (TYR), and is oxidized to form dopaquinone, which isomerizes to form dopachrome, which reacts further through 2 pathways. Firstly, the dopa pigment is decarboxylated and generates 5, 6-indoloquinone under the action of TYR; secondly, the dopa pigment forms 5, 6-dihydroxyindole carboxylic acid under the action of TRP-2, further generates 5, 6-indole quinone carboxylic acid under the catalysis of TRP-1, and finally forms true pigment from the 5, 6-indole quinone and the 5, 6-indole quinone carboxylic acid. Therefore, tyrosinase plays a significant role in the whole reaction, and inhibiting the activity of tyrosinase can effectively inhibit the formation of melanin. In the experiment of the invention, the inhibition rate of the whitening antibacterial composition of the embodiment 1-5 with the concentration of 12.5 mug/mL to tyrosinase activity is 6.69%, and after the whitening antibacterial composition of the embodiment 1-5 with the concentration of 200 mug/mL is treated for 72 hours, the inhibition rate reaches 46.69%, so that the whitening antibacterial composition has a better inhibition effect.

4. Antibacterial activity assay:

1. recovery of the strain and preparation of bacterial suspension:

inoculating small amount of activated strain into sterile liquid culture medium (100 mL), respectively, fixing on shaking table at 37deg.C, and culturing for 24 hr to obtain 10-concentration strain ⁶ -10 ⁸ The CFU/mL bacterial suspension is ready for use.

2. Determination of Minimum Inhibitory Concentration (MIC):

the dilution method is adopted: the whitening antibacterial composition of examples 1-5 was first dissolved with 5%DMSO (v/v), then 1% Tween-80 (v/v) was added, then a solution with a concentration of 320.0mg/mL was prepared with distilled water, filtered through a 0.22 μm filter membrane and used as the mother liquor of examples 1-5, and finally diluted with liquid medium in sequential ratios to a series of concentrations, i.e., 160, 80.0, 40.0, 20.0, 10.0 and 5.0mg/mL in sequence. Sequentially adding the liquid medicines in examples 1-5 and the liquid medicines diluted to different concentrations into a 96-well plate according to the order from small concentration to large concentration, wherein each well is 0.1mL; then 0.1mL of the bacterial suspension was added separately. A96-well plate was incubated in an incubator at 37℃for 24 hours with a treatment of adding 0.1mL of the bacterial suspension and 0.1mL of the liquid medium as a positive control, and a treatment of adding only 0.2mL of the liquid medium as a negative control. The lowest concentration corresponding to no bacterial growth treatment at all is the Minimum Inhibitory Concentration (MIC).

3. Determination of Minimum Bactericidal Concentration (MBC):

and (3) respectively coating a certain amount of bacterial liquid on a corresponding sterile agar plate in each treatment hole without bacterial growth, culturing in a constant temperature box at 37 ℃ for 24 hours, and taking the minimum drug concentration without bacterial growth on the plate as MBC as an observation result.

The above measurement results are shown in Table 4.

TABLE 4 determination of MIC/MBC of 10 common pathogenic bacteria for samples of examples 1-5 of the present invention

Note that: "+" indicates gram-positive and "-" indicates gram-negative; "200" indicates that MBC values cannot be observed at this concentration.

The inhibition activity of the compositions prepared in examples 1-5 of the present invention against 10 selected common pathogens was studied by the dilution method using a multiple ratio, and the results are shown in Table 4. The results show that the composition of the embodiment 1-5 has better inhibition effect on 10 common pathogenic bacteria selected by the test, particularly has the strongest inhibition effect on staphylococcus aureus, streptococcus pneumoniae, escherichia coli, streptococcus pyogenes and pseudomonas aeruginosa, and the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) are below 20.0 mg/mL. In addition, as shown by the antibacterial activity results, the compositions of examples 1 to 5 of the present invention have remarkable inhibitory effects on both gram-negative bacteria and gram-positive bacteria, and have stronger inhibitory ability on gram-positive bacteria than gram-negative bacteria.

The application embodiment of the invention is the whitening and antibacterial cosmetic containing Fuding white tea extract:

table 5 shows the raw material compositions of application examples 1-4 of the whitening and antibacterial cosmetics containing Fuding white tea extract.

TABLE 5 Components of application examples 1-4 according to the invention and weight percent

The preparation method of the whitening and antibacterial cosmetic containing the Fuding white tea extract in the application embodiments 1-4 comprises the following detailed steps:

1) According to the invention, the raw materials of the whitening antibacterial cosmetic in any one of the application examples 1-4 are prepared into various raw materials, firstly, the Fuding white tea extract, the sea buckthorn polysaccharide extract, the 1, 3-butanediol, the sodium hyaluronate and the deionized water are added into an aqueous phase pot to be stirred, and the mixture is heated to 45 ℃ after being uniformly mixed to obtain an aqueous phase material;

2) Then sequentially adding polyglycerol fatty acid ester, rhizoma Ligustici Chuanxiong extract, squalane, fructus Zanthoxyli seed antibacterial peptide, cetostearyl alcohol, ascorbyl tetraisopalmitate, stearyl alcohol and isostearyl lactoyl sodium lactate into an oil phase pot, heating to 45deg.C, stirring and mixing to obtain oil phase material;

3) Pumping the water phase material into an emulsifying pot, stirring and homogenizing, pumping the oil phase material, and homogenizing at 45deg.C for 20 min to obtain whitening antibacterial skin cream containing Fuding white tea extract.

The whitening antibacterial skin cream prepared by the application specific embodiment of the invention is used for carrying out the following performance tests:

1. sensory and physical and chemical performance index test:

the sensory and physical and chemical index measurement results of the whitening antibacterial skin cream disclosed by the invention are shown in Table 6, and the test results meet the standard requirements of QB/T1857-2013 skin cream.

TABLE 6 sensory and physicochemical index determination results of the whitening and antibacterial skin cream of the present invention

2. Efficacy test example:

the efficacy test example detects the capability of the product of the invention for whitening, resisting bacteria and protecting skin to improve skin brightness.

Group of subjects: 100 adult female volunteers, between 25 and 40 years of age, were randomized into 10 groups.

The test method comprises the following steps: after each group of subjects were cleansing the face every day in the morning and evening, after using the same amount of the same lotion, the same amount of the present invention application examples 1 to 4 and 0.1% arbutin was applied to the face, respectively, and after continuing to use for 2 weeks and 4 weeks, the improvement degree of skin brightness (L value) and pigmentation was measured by a facial skin image analyzer (VISI A-CR), the test results are shown in Table 7 in detail, and the data are the average of all the data in the same test group.

TABLE 7 whitening antibacterial skin cream of the present invention's ability to improve skin brightness

As can be seen from the data in Table 7, the skin brightness of the subjects using the whitening and antibacterial skin cream of the present invention is increased by at least 0.93% and up to 1.78% as compared with the basic value. Therefore, the product of the invention has obvious whitening effect.

3. Satisfaction score:

the skin cream of the application examples 1-4 is used for each time in the morning and evening respectively, and after three months of use, the skin cream is scored for whitening, freckle removal, inflammation diminishing, greasy feeling and moisturizing effects, and the total use effects are divided into 5 points: the score of 5 is the highest score, which shows good and very satisfactory; 4 is better; 3, dividing into good parts; a score of 3 or less is not good and unacceptable. And (3) injection: the rules for the greasy feel score are: 5 minutes, no oil, good use feeling and very satisfactory; 4, the use feeling is good; 3, the use feeling is good; below 3 minutes, the product was very greasy, and had poor feel and was unacceptable. The average scores are shown below, and the results are shown in Table 8.

TABLE 8 satisfaction score test of whitening antibacterial skin care using the products of examples 1-4 of the present invention

Panelist	Whitening skin	Freckle removing	Anti-inflammatory agent	Greasy feel	Moisturizing and moisturizing
						Application example 1	4.5	3.7	4.1	4.6	4.7
Application example 2	4.6	3.6	4.2	4.6	4.7
						Application example 3	4.7	3.9	4.5	4.8	4.9
Application example 4	4.6	3.5	4.3	4.5	4.8

。

Claims

1. The whitening and antibacterial cosmetic composition containing the Fuding white tea extract is characterized by comprising the following raw materials of the Fuding white tea extract, the ligusticum wallichii extract, the sea buckthorn polysaccharide extract and the pepper seed antibacterial peptide in a mass ratio of 42-48: 40-46: 7.5 to 11.5:0.25 to 0.5;

the preparation method of the Fuding white tea extract comprises the following steps: the Fuding white tea is placed in an extraction tank, water is added, and the mass volume ratio of the Fuding white tea to the water is 1g: 30-50 mL, and then heating to boiling condition for extraction for 10-20 min; extracting for 2-3 times according to the method, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract, thus obtaining the Fuding white tea extract;

the preparation method of the ligusticum wallichii extract comprises the following steps: pulverizing rhizoma Ligustici Chuanxiong root to below 20 mesh, and ultrasonic extracting with 50-90% ethanol at 60-75deg.C for 50-70 min; ultrasonic extracting for 2-3 times under the same condition, combining the obtained extracting solutions, filtering and concentrating the combined extracting solutions to obtain a pasty crude extract to obtain the ligusticum chuanxiong hort extract;

The preparation method of the sea buckthorn polysaccharide extract comprises the following steps: crushing dry sea buckthorn fruits, sieving with a 40-mesh sieve, and then adding petroleum ether with the volume of 10-15 times for reflux extraction for 2-3 times, wherein the extraction time is 40-80 min each time; mixing the extracts, removing fat-soluble substances, and performing suction filtration to obtain filter residues for later use;

the residue obtained was purified according to a ratio of 1g: distilled water is added into the mixture of 20-30 mL of the liquid to be extracted under the auxiliary ultrasonic wave at the temperature of 65-75 ℃, and the obtained extracting solution is concentrated to 1g/mL; adding 3-5 times volume of absolute ethyl alcohol into the obtained concentrate, cooling to 4 ℃ and standing for 10-14 h to generate precipitate, then carrying out precipitation centrifugation, removing protein from the solution obtained after centrifugation, drying the substance obtained after protein removal in a drying oven at 35-45 ℃ to obtain the sea buckthorn crude polysaccharide extract;

c. adding 10 times of NaOH solution with concentration of 0.5% into the defatted pricklyash seed powder, and stirring for 30 min; centrifuging at 8000 r.min for 20min, and collecting supernatant; adding 10 times of NaOH solution with the concentration of 0.5% into the centrifuge tube again, and repeating the above steps; combining the supernatants obtained by 2 times of centrifugation, and freeze-drying after combining to obtain the pricklyash seed protein;

2. A whitening and antibacterial cosmetic containing Fuding white tea extract is characterized in that: the whitening and antibacterial cosmetic composition according to claim 1 comprises 1-10% by mass of the cosmetic raw material composition.

3. The whitening and antibacterial cosmetic containing the fuding white tea extract according to claim 2, wherein: the whitening antibacterial cosmetic comprises the following raw materials in percentage by mass: 0.44 to 1.76 percent of Fuding white tea extract, 0.115 to 0.46 percent of sea buckthorn polysaccharide extract, 0.44 to 1.76 percent of szechuan lovage rhizome extract, 0.005 to 0.02 percent of pricklyash peel seed antibacterial peptide, 1 to 5 percent of 1, 3-butanediol, 1 to 1.2 percent of sodium hyaluronate, 1 to 1.3 percent of polyglycerol fatty acid ester, 1 to 1.8 percent of squalane, 1 to 1.8 percent of cetostearyl alcohol, 0.04 to 0.1 percent of tetraisopalmitate of ascorbic acid, 1 to 1.8 percent of stearyl alcohol and 1 to 1.8 percent of isostearyl lactoyl sodium lactate, and the balance of deionized water, wherein the sum of the percentage of the components is 100 percent.

4. The whitening and antibacterial cosmetic containing the fuding white tea extract according to claim 3, wherein the raw materials of the whitening and antibacterial cosmetic comprise the following components: 0.88% of Fuding white tea extract, 0.23% of sea buckthorn polysaccharide extract, 0.88% of ligusticum wallichii extract, 0.01% of pepper seed antibacterial peptide, 3% of 1, 3-butanediol, 1.2% of sodium hyaluronate, 1.1% of polyglycerol fatty acid ester, 1.5% of squalane, 1.2% of cetostearyl alcohol, 0.05% of ascorbyl tetraisopalmitate, 1.2% of stearyl alcohol and 1.2% of isostearyl lactoyl sodium lactate, and the balance of deionized water, wherein the sum of the percentages of the components is 100%.

5. The preparation method of the whitening antibacterial cosmetic containing the Fuding white tea extract is characterized by comprising the following steps of:

1) According to the raw material composition of the whitening antibacterial cosmetic of claim 3, various raw materials are prepared, firstly, the Fuding white tea extract, the sea buckthorn polysaccharide extract, the 1, 3-butanediol, the sodium hyaluronate and the deionized water are added into a water phase pot for stirring, and the mixture is heated to 40-50 ℃ after being uniformly mixed to obtain a water phase material;