CN114755284A - Double-channel spectrum detection device for capillary electrophoresis - Google Patents
Double-channel spectrum detection device for capillary electrophoresis Download PDFInfo
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- CN114755284A CN114755284A CN202210543045.8A CN202210543045A CN114755284A CN 114755284 A CN114755284 A CN 114755284A CN 202210543045 A CN202210543045 A CN 202210543045A CN 114755284 A CN114755284 A CN 114755284A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Abstract
The invention relates to the field of electrophoresis detection, and particularly discloses a double-channel spectrum detection device for capillary electrophoresis, which comprises an electrophoresis capillary, wherein a first detection mechanism and a second detection mechanism are arranged on the outer side of the electrophoresis capillary, the first detection mechanism and the second detection mechanism are positioned on the outer side of the electrophoresis capillary and are arranged in a mutually vertical manner, and one ends of the first detection mechanism and the second detection mechanism, which are positioned close to the electrophoresis capillary, are connected with a second dimmer. The sensitivity of the micro-sample and the trace sample is improved by using the spectrum analyzer and the laser to detect the micro-sample and the trace sample, and the experimental reliability is improved; the detection targets are expanded, and the working efficiency of the instrument is improved; fluorescence cross is reduced, and the accuracy of the experiment is improved; the manufacturing cost of the dye kit is reduced, the detection items are increased, and the laser and fluorescence signals are transmitted to the capillary and the spectrum analyzer through the optical fiber, so that the detection accuracy can be conveniently controlled.
Description
Technical Field
The invention relates to the field of electrophoresis detection, in particular to a double-channel spectrum detection device for capillary electrophoresis.
Background
The international existing standard technology adopts a single laser, single detector and multi-fluorescence capillary gene detection mode, and is a gold standard which can be realized in the fields of clinical medicine, judicial authorities, agricultural research and the like at present. Because single laser is adopted to excite multiple fluorescent dyes, more than six different fluorescent dyes are difficult to excite in the method, and the excitation sites are 24 sites at most; however, in all the projects needing to be tested, the site needing excitation is generally 60-100, so that the prior art cannot meet the market application.
Disclosure of Invention
Aiming at the existing problems, the invention provides a dual-channel spectrum detection device for capillary electrophoresis, which can effectively solve the problems in the background technology by matching.
In order to solve the problems, the invention adopts the following technical scheme:
the utility model provides a binary channels spectrum detection device of capillary electrophoresis, includes the electrophoresis capillary, the outside of electrophoresis capillary is provided with first detection mechanism and second detection mechanism, first detection mechanism reaches second detection mechanism is located the outside of electrophoresis capillary is mutually perpendicular lays, first detection mechanism reaches second detection mechanism is located and is close to the one end of electrophoresis capillary is connected with the second dimer piece.
As a still further scheme of the invention: a dichroscope is arranged on one side of the second light condenser inside the second detection mechanism, a reflecting mirror is arranged right below the dichroscope, a cut-off filter is arranged on one side of the dichroscope far away from the second light condenser, and a filter is arranged right below the cut-off filter.
As a still further scheme of the invention: and a spectrum analyzer is arranged on one side of the cut-off filter, a first light-gathering sheet is arranged between the cut-off filter and the spectrum analyzer, and a laser is arranged on one side of the filter, which is far away from the reflector.
As a still further scheme of the invention: the first detection mechanism and the second detection mechanism are equal in structure, and at least one group of the first detection mechanism and the second detection mechanism is arranged on the outer side of the electrophoresis capillary.
As a still further scheme of the invention: the dichroscope and the reflector are obliquely arranged in the second detection mechanism, and the inclination angles of the dichroscope and the reflector are equal.
As a still further scheme of the invention: the axis position of the spectrum analyzer coincides with the central position of the first light-gathering piece, and the axis position of the laser coincides with the central position of the optical filter.
Compared with the prior art, the invention has the beneficial effects that:
1. the sensitivity of the micro-sample and the trace sample is improved by using the spectrum analyzer and the laser to detect the micro-sample and the trace sample, and the experimental reliability is improved; the detection targets are expanded, and the working efficiency of the instrument is improved; fluorescence cross is reduced, and the accuracy of the experiment is improved; the manufacturing cost of the dye kit is reduced, the detection items are increased, and the laser and fluorescence signals are transmitted to the capillary and the spectrum analyzer through the optical fiber, so that the detection accuracy can be conveniently controlled.
2. The collection of fluorescence of different wavelength can be realized to at least a set of first detection mechanism and the second detection mechanism that sets up in the electrophoresis capillary outside, and whole device adopts confined optical fiber transmission mode, has solved the combination reflector reflection that current instrument adopted and has made the instrument unable realize portable problem.
Drawings
FIG. 1 is a schematic structural diagram of a dual-channel spectrum detection device for capillary electrophoresis;
FIG. 2 is a schematic diagram of light path transmission in a dual-channel spectrum detection device for capillary electrophoresis.
In the figure: 1. a first detection mechanism; 2. a second detection mechanism; 3. an electrophoresis capillary; 4. a spectrum analyzer; 5. a laser; 6. a first light-condensing sheet; 7. a cut-off filter; 8. a dichromatic mirror; 9. a second polarizer; 10. a mirror; 11. and (3) a filter.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
As shown in fig. 1-2, the present embodiment provides a dual-channel spectrum detection device for capillary electrophoresis, comprising an electrophoresis capillary 3, the outer side of an electrophoresis capillary 3 is provided with a first detection mechanism 1 and a second detection mechanism 2, the first detection mechanism 1 and the second detection mechanism 2 are positioned at the outer side of the electrophoresis capillary 3 and are arranged in a mutually perpendicular way, one end of the first detection mechanism 1 and the second detection mechanism 2, which are positioned close to the electrophoresis capillary 3, is connected with a second dimmer sheet 9, one side of the second dimmer sheet 9 is positioned inside the second detection mechanism 2 and is provided with a dichroscope 8, a reflecting mirror 10 is arranged right below the dichroscope 8, the dichroscope 8 and the reflecting mirror 10 are arranged in an inclined way inside the second detection mechanism 2, and the inclination angles are equal, the dichroic mirror 8 is provided with a cut-off filter 7 at one side far away from the second dimmer 9, and a filter 11 is arranged right below the cut-off filter 7.
As shown in fig. 1-2, a spectrum analyzer 4 is disposed on one side of a cut-off filter 7, a first light-collecting sheet 6 is disposed between the cut-off filter 7 and the spectrum analyzer 4, the axial position of the spectrum analyzer 4 coincides with the center position of the first light-collecting sheet 6, a laser 5 is disposed on one side of a filter 11 away from a reflector 10, the axial position of the laser 5 coincides with the center position of the filter 11, a first detection mechanism 1 and a second detection mechanism 2 are identical in structure, and at least one set of the first detection mechanism 1 and the second detection mechanism 2 is disposed outside an electrophoresis capillary 3. The spectrum analyzer 4 and the laser 5 are both made of existing products, such as: the model AQ6360 is selected for the spectrum analyzer 4, and the model LM12-AOS200 is selected for the laser 5.
The working principle of the invention is as follows: the laser 5 emits a beam of laser with a specific wavelength, the laser irradiates the electrophoresis capillary 3 under the guiding action of the optical filter 11, the reflector 10, the dichroscope 8 and the second fluorescence sheet 9, different dyes are marked on primers of different target points on the electrophoresis capillary 3, then PCR (polymerase chain reaction) primers marked with multiple fluorescent dyes are mixed and amplified, the amplified product enters the electrophoresis capillary 3 through denaturation electrophoresis, then fluorescence in a specific range in the electrophoresis capillary 3 is excited under the action of the laser emitted by the laser 5, the fluorescence is reflected to the dichroscope 8 and then is transmitted to the cut-off optical filter 7 to irradiate the inside of the optical spectrum analyzer 4 under the action of the first light-gathering sheet 6, and the information of an original sample is obtained by collecting fluorescence signals in the specific range.
It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions, and furthermore, the terms "comprise", "include", or any other variation thereof are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a binary channels spectrum detection device of capillary electrophoresis, includes electrophoresis capillary (3), its characterized in that, the outside of electrophoresis capillary (3) is provided with first detection mechanism (1) and second detection mechanism (2), first detection mechanism (1) reaches second detection mechanism (2) are located the outside of electrophoresis capillary (3) is mutually perpendicular and lays, first detection mechanism (1) reaches second detection mechanism (2) are located and are close to the one end of electrophoresis capillary (3) is connected with second dimer light piece (9).
2. The dual-channel spectrum detection device for capillary electrophoresis according to claim 1, wherein a dichroic mirror (8) is disposed inside the second detection mechanism (2) on one side of the second light condenser (9), a reflecting mirror (10) is disposed right below the dichroic mirror (8), a cut-off filter (7) is installed on one side of the dichroic mirror (8) far away from the second light condenser (9), and a filter (11) is disposed right below the cut-off filter (7).
3. The dual-channel spectrum detection device for capillary electrophoresis according to claim 2, wherein a spectrum analyzer (4) is disposed on one side of the cut-off filter (7), a first light-focusing sheet (6) is disposed between the cut-off filter (7) and the spectrum analyzer (4), and a laser (5) is disposed on one side of the filter (11) away from the reflector (10).
4. The dual-channel spectrum detection device for capillary electrophoresis as claimed in claim 1, wherein the first detection mechanism (1) and the second detection mechanism (2) are equal in structure, and at least one set of the first detection mechanism (1) and the second detection mechanism (2) is disposed outside the electrophoresis capillary (3).
5. The dual-channel spectrum detection device of capillary electrophoresis according to claim 2, wherein the dichroic mirror (8) and the reflecting mirror (10) are arranged in an inclined manner inside the second detection mechanism (2), and the inclined angles are equal.
6. The apparatus for dual-channel spectrum detection of capillary electrophoresis according to claim 3, wherein the axis position of the spectrum analyzer (4) coincides with the center position of the first light-gathering sheet (6), and the axis position of the laser (5) coincides with the center position of the optical filter (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210543045.8A CN114755284A (en) | 2022-05-18 | 2022-05-18 | Double-channel spectrum detection device for capillary electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210543045.8A CN114755284A (en) | 2022-05-18 | 2022-05-18 | Double-channel spectrum detection device for capillary electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114755284A true CN114755284A (en) | 2022-07-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210543045.8A Pending CN114755284A (en) | 2022-05-18 | 2022-05-18 | Double-channel spectrum detection device for capillary electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114755284A (en) |
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2022
- 2022-05-18 CN CN202210543045.8A patent/CN114755284A/en active Pending
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