CN114753985A - Self-adaptive air type liquid transfer pump, high-precision liquid transfer method and storage medium - Google Patents
Self-adaptive air type liquid transfer pump, high-precision liquid transfer method and storage medium Download PDFInfo
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- CN114753985A CN114753985A CN202210437060.4A CN202210437060A CN114753985A CN 114753985 A CN114753985 A CN 114753985A CN 202210437060 A CN202210437060 A CN 202210437060A CN 114753985 A CN114753985 A CN 114753985A
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B17/00—Pumps characterised by combination with, or adaptation to, specific driving engines or motors
- F04B17/03—Pumps characterised by combination with, or adaptation to, specific driving engines or motors driven by electric motors
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B13/00—Pumps specially modified to deliver fixed or variable measured quantities
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B49/00—Control, e.g. of pump delivery, or pump pressure of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for, or of interest apart from, groups F04B1/00 - F04B47/00
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B53/00—Component parts, details or accessories not provided for in, or of interest apart from, groups F04B1/00 - F04B23/00 or F04B39/00 - F04B47/00
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B53/00—Component parts, details or accessories not provided for in, or of interest apart from, groups F04B1/00 - F04B23/00 or F04B39/00 - F04B47/00
- F04B53/08—Cooling; Heating; Preventing freezing
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- General Engineering & Computer Science (AREA)
- Reciprocating Pumps (AREA)
Abstract
The invention discloses a self-adaptive air-type liquid transfer pump, a high-precision liquid transfer method and a storage medium. According to the invention, the heating device and the refrigerating device are arranged on the pump body, and when the temperature of the liquid to be pipetted is not equal to the temperature of the air in the air section in the pump body, the air temperature in the air section is adjusted through the heating device or the refrigerating device until the temperature of the air in the air section is equal to the temperature of the liquid to be pipetted, so that the problem of inaccurate pipetting amount caused by the change of the volume of the air section after the piston assembly moves to a preset position for pipetting during pipetting is avoided.
Description
Technical Field
The invention relates to the technical field of liquid transfer pumps, in particular to a self-adaptive air type liquid transfer pump, a high-precision liquid transfer method and a storage medium.
Background
The air type liquid displacement pump has the characteristics of no need of liquid filling, lightness, compact structure, no maintenance, low cross contamination rate and the like, and is widely applied to related detection instruments in the IVD industry. Especially, because a section of air column exists between the piston sealing piece and the liquid, when the air column is matched with the Tip head for use, the transferred liquid does not contact with the sealing piece, and the risk of cross contamination is reduced to the maximum extent by replacing the Tip head when the liquid is transferred each time. Therefore, the method is widely used in the fields of sensitive cross contamination, especially molecular diagnosis.
However, in the molecular diagnosis field, the liquid to be transferred often has a comparatively obvious difference in temperature with ambient temperature, when transferring these liquids, continuously inhales the flowing back, and the air section in the liquid-transfering pump body exchanges the heat with liquid to change the volume, influence the degree of accuracy that moves the liquid, instrument equipment application of sample is littleer and more, and when moving liquid speed is faster and faster, this problem is just more prominent, can influence molecular diagnosis's accuracy even.
Accordingly, there is a need for improvements and developments in the art.
Disclosure of Invention
The invention aims to solve the technical problem that in the prior art, a self-adaptive air type liquid-transferring pump, a high-precision liquid-transferring method and a storage medium are provided to solve the problem that in the prior art, liquid to be transferred is inaccurate when the temperature difference exists between the liquid to be transferred and the ambient temperature.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
an adaptive air-based ambulatory pump comprising a pump body having an interior chamber therein, a piston assembly slidably disposed within the interior chamber, an air section between the piston assembly and the bottom of the interior chamber, the adaptive air-based ambulatory pump further comprising:
the heating device is arranged on the pump body and is used for heating the pump body when the temperature of the liquid to be pipetted is higher than that of the pump body, so that the temperature of the air in the air section is equal to that of the liquid to be pipetted;
the refrigerating device is arranged on the pump body and used for refrigerating the pump body when the temperature of the liquid to be transferred is lower than that of the pump body, so that the air temperature in the air section is equal to that of the liquid to be transferred.
The self-adaptive air type liquid displacement pump is characterized in that the heating device is an electric heating film.
The adaptive air-type liquid displacement pump, wherein the refrigeration device includes:
the semiconductor refrigerating piece is connected with the pump body;
the moisture insulation piece is connected with one side, back to the pump body, of the semiconductor refrigeration sheet; and
and the heat dissipation block is connected with one side of the moisture insulation piece, which faces back to the semiconductor refrigeration piece.
The self-adaptation air formula liquid pump that moves, wherein, the radiating block is connected with the bolt, the bolt is kept away from the one end of radiating block with the pump body is connected, the cover is equipped with the heat insulating block on the screw rod of bolt.
The self-adaptive air type liquid displacement pump further comprises a control assembly, and the control assembly is respectively connected with the piston assembly, the heating device and the refrigerating device;
an environment temperature sensor is arranged on one side, back to the pump body, of the control assembly and is connected with the control assembly;
the pump body is provided with a pump body temperature sensor, and the pump body temperature sensor is connected with the control assembly.
The adaptive air displacement pump, wherein the piston assembly comprises:
the motor is connected with the pump body;
the lead screw is connected with the motor;
the piston is connected to one end of the lead screw and is arranged in the inner cavity in a sliding manner;
the sliding block is connected with one end, far away from the piston, of the lead screw; and
the guide rod is parallel to the lead screw, and the sliding block is connected with the guide rod in a sliding mode.
A method for high precision pipetting of an adaptive air-based pipetting pump as described in any of the above, the method comprising:
respectively acquiring the temperature of liquid to be moved and the ambient temperature;
when the temperature of the liquid to be pipetted is higher than the ambient temperature, controlling the heating device to heat the pump body to be equal to the temperature of the liquid to be pipetted, and when the temperature of the liquid to be pipetted is lower than the ambient temperature, controlling the refrigerating device to cool the pump body to be equal to the temperature of the liquid to be pipetted;
and controlling the pump body to perform pipetting.
The high-precision pipetting method, wherein when the temperature of the liquid to be pipetted is higher than the ambient temperature, controlling the heating device to heat the pump body to be equal to the temperature of the liquid to be pipetted comprises:
when the temperature of the liquid to be transferred is higher than the environmental temperature, controlling a heating device to heat the pump body, and collecting the temperature of the pump body at intervals of preset interval time;
and when the weighted average value of the temperature values of the pump body acquired five times continuously is larger than the temperature of the liquid to be moved, controlling the heating device to stop heating.
The high-precision pipetting method, wherein when the temperature of the liquid to be pipetted is lower than the ambient temperature, controlling the refrigerating device to cool the pump body to be equal to the temperature of the liquid to be pipetted comprises:
when the temperature of the liquid to be moved is lower than the environmental temperature, controlling a refrigerating device to refrigerate the pump body, and collecting the temperature of the pump body at intervals of preset interval time;
and when the weighted average value of the temperature values of the pump body acquired five times continuously is smaller than the temperature of the liquid to be moved, controlling the refrigerating device to stop refrigerating.
A storage medium storing a computer program executable for implementing a control method of a plunger pump as claimed in any one of the preceding claims.
Has the advantages that: according to the invention, the heating device and the refrigerating device are arranged on the pump body, and when the temperature of the liquid to be pipetted is not equal to the temperature of the air in the air section in the pump body, the air temperature in the air section is adjusted through the heating device or the refrigerating device until the temperature of the air in the air section is equal to the temperature of the liquid to be pipetted, so that the problem of inaccurate pipetting amount caused by the change of the volume of the air section after the piston assembly moves to a preset position for pipetting during pipetting is avoided.
Drawings
FIG. 1 is an exploded view of the adaptive air-based pipetting pump of the present invention;
FIG. 2 is a schematic view of the structure at A in FIG. 1;
FIG. 3 is a schematic cross-sectional view of the adaptive air-operated fluid displacement pump provided by the present invention;
FIG. 4 is an exploded view of the adaptive air displacement pump according to the present invention;
FIG. 5 is a schematic block diagram of the flow of the high-precision pipetting method provided by the invention;
the labels in the figures are: 1. a pump body; 2. an inner cavity; 21. an air section; 3. a piston assembly; 31. a motor; 32. a lead screw; 33. a piston; 34. a slider; 35. a guide bar; 4. a heating device; 41. an electrothermal film; 42. pressing a plate; 5. a refrigeration device; 51. a semiconductor refrigeration sheet; 52. a moisture barrier; 53. a heat dissipating block; 6. a connecting rod; 7. a pipeline; 8. a heat insulation block; 9. a control component; 10. an ambient temperature sensor; 11. a pump body temperature sensor; 12. a metal housing; 13. a heat-insulating layer; 14. and (4) bolts.
Detailed Description
The present invention provides an adaptive air-based pipetting pump, a high-precision pipetting method and a storage medium, and in order to make the objects, technical solutions and effects of the present invention clearer and clearer, the present invention will be described in further detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It should also be noted that the same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is only for convenience of describing the present invention and simplifying the description, but it is not intended to indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limiting the present patent, and the specific meaning of the terms may be understood by those skilled in the art according to specific circumstances.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
The invention will be further explained by the description of the embodiments with reference to the drawings.
The embodiment provides an adaptive air-based pipetting pump, a high-precision pipetting method and a storage medium, and as shown in fig. 1 and fig. 3, the adaptive air-based pipetting pump comprises a pump body 1, an inner cavity 2 is arranged in the pump body 1, and a piston assembly 3 is slidably arranged in the inner cavity 2; an air section 21 is arranged between the piston assembly 3 and the bottom of the inner cavity 2, the pump body 1 is further connected with a connecting rod 6, and the connecting rod 6 is used for installing a suction head to suck or discharge solution, for example, the suction head is a tip head; a pipeline 7 is connected between the inner cavity 2 and the connecting rod 6, and the air section 21 is positioned at one end of the inner cavity 2 close to the pipeline 7. And when pipetting, the liquid to be pipetted enters the pipeline 7 from the connecting rod and does not enter the air section 21; when liquid needs to be transferred, the piston assembly 3 is controlled to perform liquid pumping action to suck a liquid to be transferred into the pipeline 7 through the suction head on the connecting rod 6, and then after the suction head is aligned with a liquid transfer target container of the liquid to be transferred, the piston assembly 3 performs liquid pushing action to discharge the liquid to be transferred in the pipeline 7, so that liquid is transferred through the suction and discharge action of the pump body 1.
The self-adaptive air type liquid displacement pump further comprises a heating device 4 and a refrigerating device 5, wherein the heating device 4 and the refrigerating device 5 are both arranged on the pump body 1; the heating device 4 is used for heating the pump body 1 when the temperature of the liquid to be transferred is higher than that of the pump body 1, so that the temperature of the air in the air section 21 is equal to that of the liquid to be transferred; the refrigerating device 5 is used for refrigerating the pump body 1 when the temperature of the liquid to be transferred is lower than that of the pump body 1, so that the temperature of the air in the air section 21 is equal to that of the liquid to be transferred. Since the temperature of the liquid to be pipetted may be different from the ambient temperature, and at this time, the temperature of the liquid to be pipetted is also not equal to the temperature of the air in the air section 21 in the pump body 1, in order to avoid that the volume of the air section 21 is changed due to the heating or cooling of the air in the air section 21 by the liquid to be pipetted when the liquid is aspirated and discharged, and the amount of the aspirated and discharged liquid is not accurate, in this embodiment, the temperature of the air in the air section 21 is adjusted to be equal to the temperature of the liquid to be pipetted by the heating device 4 or the cooling device 5 before pipetting, so that the pipetting amount of the liquid to be pipetted by the pump body 1 is more accurate when the liquid to be pipetted is aspirated and discharged.
According to the invention, the heating device 4 and the refrigerating device 5 are arranged on the pump body 1, and when the temperature of the liquid to be transferred is not equal to the temperature of the air in the air section 21 in the pump body 1, the air temperature in the air section 21 is adjusted through the heating device 4 or the refrigerating device 5 until the air temperature in the air section 21 is equal to the temperature of the liquid to be transferred, so that the problem that the liquid transfer amount is not accurate due to the volume change of the air section 21 after the piston assembly 3 moves to a preset position for liquid pumping during liquid transfer is avoided.
In a specific embodiment, as shown in fig. 3, the heating device 4 is an electrothermal film 41, a pressing plate 42 is disposed on a side of the electrothermal film 41 opposite to the pump body 1, and the pressing plate 42 is connected to the pump body 1 through a bolt and clamps and attaches the electrothermal film 41 to the pump body 1.
In another embodiment, as shown in fig. 2, the refrigerating device 5 includes a semiconductor refrigerating sheet 51, a moisture insulating member 52, and a heat dissipating block 53; the semiconductor refrigeration piece 51 is connected with the pump body 1, the moisture-insulating piece 52 is connected with one side, back to the pump body 1, of the semiconductor refrigeration piece 51, and the heat dissipation block 53 is connected with one side, back to the semiconductor refrigeration piece 51, of the moisture-insulating piece 52, namely, the semiconductor refrigeration piece 51, the moisture-insulating piece 52 and the heat dissipation block 53 are sequentially connected; when the semiconductor refrigerating sheet 51 operates, the semiconductor refrigerating sheet refrigerates towards one side of the pump body 1 and radiates heat from one side of the pump body 1; the heat dissipation block 53 is used for accelerating the heat dissipation of the semiconductor chilling plate 51 during operation so as to improve the chilling efficiency of the semiconductor chilling plate 51; the moisture barrier 52 is used for isolating condensed water and water vapor in air, and preventing the semiconductor chilling plate 51 from being corroded and damaged, and for example, the moisture barrier 52 is made of rubber.
Further, the heat dissipation block 53 is connected with a bolt 14, and the bolt 14 is sequentially connected with the heat dissipation block 53, the moisture insulating member 52, the semiconductor chilling plate 51 and the pump body 1, so as to fix the heat dissipation block 53, the moisture insulating member 52 and the semiconductor chilling plate 51 on the pump body 1; the screw of the bolt 14 is sleeved with a heat insulation block 8, for example, the heat insulation block 8 is made of plastic; the heat insulation block 8 is used for heat insulation to prevent the heat dissipated by the heat dissipation block 53 from being transmitted back to the pump body 1 through the bolt 14, which results in reduction of refrigeration efficiency.
The self-adaptive air type liquid displacement pump further comprises a control assembly 9, wherein the control assembly 9 is respectively connected with the piston assembly 3, the heating device 4 and the refrigerating device 5 so as to control the operation of the piston assembly 3, the heating device 4 and the refrigerating device 5; for example, the control component 9 is a PCBA board component.
As shown in fig. 4, an ambient temperature sensor 10 is disposed on a side of the control assembly 9 facing away from the pump body 1, and the ambient temperature sensor 10 is connected to the control assembly 9; a pump body temperature sensor 11 is arranged on the pump body 1, and the pump body temperature sensor 11 is connected with the control assembly 9; the environment temperature sensor 10 is used for detecting the temperature of the external environment when the adaptive air-type liquid-transferring pump does not work, so as to judge whether the environment temperature is equal to the temperature of the liquid to be transferred; of course, when the adaptive air-based liquid-displacement pump is not operating, the air temperature in the air section 21 is defaulted to be equal to the temperature of the external environment; the pump body temperature sensor 11 is used for detecting the real-time temperature of the pump body 1 when the adaptive air-type liquid-transfering pump works, and the real-time temperature of the air in the air section 21 is defaulted to be equal to the temperature of the pump body 1 when the adaptive air-type liquid-transfering pump works. When the control assembly 9 controls the heating device 4 or the refrigerating device 5 to adjust the temperature of the pump body 1, the pump body temperature sensor 11 detects the temperature of the pump body 1 in real time and feeds back the obtained temperature value to the control assembly 9 in real time, and the control assembly 9 controls the heating device 4 or the refrigerating device 5 to work according to a feedback result so that the temperature of the pump body 1 is adjusted to be infinitely close to the temperature of the liquid to be moved. For example, when the temperature value fed back to the control assembly 9 by the pump body temperature sensor 11 is still lower than the temperature of the liquid to be removed during the process of heating the pump body 1 by the heating device 4, the control assembly 9 continues to control the heating device 4 to heat the pump body 1, thereby continuously cycling back and forth to gradually adjust the temperature of the pump body 1 to be infinitely close to the temperature of the liquid to be removed.
A metal shell 12 is further arranged on the periphery of the pump body 1 to protect and support the pump body 1; an insulating layer 13 is arranged on the inner side of the metal shell 12, for example, the insulating layer 13 is made of insulating cotton with the thickness of 2 mm; through setting up heat preservation 13 to reduce heat-conduction and heat radiation, avoid producing too big contact heat conduction loss.
The piston assembly 3 comprises a motor 31, a lead screw 32, a piston 33, a slider 34 and a guide rod 35, the motor 31 is connected with the pump body 1, the lead screw 32 is connected with the motor 31, the lead screw 32 penetrates through the motor 31, and the lead screw 32 is in threaded connection with the motor 31; the piston 33 is connected to one end of the lead screw 32, and the piston 33 is slidably arranged in the inner cavity 2; the air section 21 is arranged between one side of the piston 33, which faces away from the screw 32, and the bottom of the inner cavity 2; the sliding block 34 is fixedly connected with one end of the lead screw 32 far away from the piston 33; the guide rod 35 is fixed on the pump body 1, the guide rod 35 is parallel to the lead screw 32, and the slider 34 is slidably connected to the guide rod 35, so that the lead screw 32 cannot rotate under the driving of the motor 31, but can only move along the length direction of the guide rod 35. Specifically, when the motor 31 is operated, the lead screw 32 rotates relative to the driving nut of the motor 31, but the lead screw 32 is limited by the slide block 34 and cannot rotate, so that the rotation of the lead screw 32 is converted into a linear movement along the length direction of the guide rod 35, and the lead screw 32 further drives the piston 33 to slide in the inner cavity 2, so as to realize a suction and discharge action and transfer a liquid to be transferred.
The invention also provides a high-precision pipetting method based on the adaptive air-type pipetting pump, which comprises the following steps as shown in fig. 5:
s10, respectively acquiring the temperature of the liquid to be moved and the ambient temperature;
s20, when the temperature of the liquid to be pipetted is higher than the ambient temperature, controlling the heating device to heat the pump body to be equal to the temperature of the liquid to be pipetted, and when the temperature of the liquid to be pipetted is lower than the ambient temperature, controlling the refrigerating device to cool the pump body to be equal to the temperature of the liquid to be pipetted;
and S30, controlling the pump body to transfer liquid.
Specifically, the temperature of the liquid to be transferred is detected, and the external main control end acquires the temperature value of the liquid to be transferred and sends the temperature value as a temperature parameter to the control component of the adaptive air-type liquid transfer pump. The self-adaptive air type liquid transferring pump detects and acquires the ambient temperature through the ambient temperature sensor, then compares the received temperature parameter with the ambient temperature, when the temperature of the liquid to be transferred is higher than the ambient temperature, the control assembly controls the heating device to heat the pump body until the temperature of the pump body is equal to the temperature of the liquid to be transferred, and controls the heating device to stop heating, and at the moment, the temperature of the air in the air section is equal to the temperature of the liquid to be transferred; when the temperature of the liquid to be moved is lower than the environmental temperature, the control assembly controls the refrigerating device to refrigerate the pump body until the temperature of the pump body is equal to that of the liquid to be moved, the refrigerating device is controlled to stop refrigerating, and at the moment, the temperature of the air in the air section is equal to that of the liquid to be moved; the control assembly then controls the piston assembly to operate to aspirate and discharge the object to be pipetted for pipetting.
Further, when the temperature of the liquid to be pipetted is higher than the ambient temperature, controlling the heating device to heat the pump body to be equal to the temperature of the liquid to be pipetted includes:
when the temperature of the liquid to be transferred is higher than the environmental temperature, controlling a heating device to heat the pump body, and collecting the temperature of the pump body at intervals of preset interval time;
and when the weighted average value of the temperature values of the pump body acquired five times continuously is greater than the temperature of the liquid to be moved, controlling the heating device to stop heating.
Specifically, when the temperature of the liquid to be moved is judged to be higher than the ambient temperature, the control assembly controls the heating device to heat the pump body, and collects the temperature of the pump body through the pump body temperature sensor at intervals of a preset interval, for example, the preset interval is 2 seconds; and when the weighted average value of the temperature values of the pump body acquired five times continuously is greater than the temperature of the liquid to be moved, the preset temperature is considered to be reached, and the control assembly controls the heating device to stop heating.
Further, when the temperature of the liquid to be transferred is lower than the ambient temperature, controlling the refrigeration device to cool the pump body to be equal to the temperature of the liquid to be transferred includes:
when the temperature of the liquid to be moved is lower than the environmental temperature, controlling a refrigerating device to refrigerate the pump body, and collecting the temperature of the pump body at intervals of preset intervals;
and when the weighted average value of the temperature values of the pump body acquired five times continuously is smaller than the temperature of the liquid to be moved, controlling the refrigerating device to stop refrigerating.
Specifically, when the temperature of the liquid to be moved is lower than the ambient temperature, the control assembly controls the refrigerating device to refrigerate the pump body, and collects the temperature of the pump body through the pump body temperature sensor at intervals of a preset interval, for example, the preset interval is 2 seconds; when the weighted average value of the temperature values of the pump body acquired five times continuously is smaller than the temperature of the liquid to be moved, the preset temperature is considered to be reached, and the control assembly controls the refrigerating device to stop refrigerating the pump body.
Furthermore, after the temperature of the pump body is heated or cooled to the preset temperature, the temperature of the pump body exchanges heat with the outside in a radiation mode, a conduction mode and the like along with the lapse of time, so that the temperature of the pump body deviates from the preset temperature, and therefore, after the temperature of the pump body deviates from the preset temperature, the heating device or the cooling device needs to be controlled to work to adjust the temperature of the pump body until the temperature of the pump body reaches the preset temperature; wherein the preset temperature is equal to the temperature of the liquid to be pipetted.
In one embodiment, initially, it is determined that the temperature of the liquid to be transferred is higher than the ambient temperature, and then the control assembly controls the heating device to start, heating the pump body, starting to increase the temperature of the pump body at the moment, determining that the pump body reaches the preset temperature until the weighted average value of the temperature values of the pump body measured for five times continuously is greater than the preset temperature, controlling the heating device to stop heating, and continuing to increase the temperature of the pump body under the residual heat at the moment, but over time, the temperature of the pump body dissipates heat by radiation, conduction, etc. to cause a temperature drop, when the temperature is reduced, the heating device starts to be started again to heat the temperature of the pump body to the preset temperature, the pump body continuously dissipates heat and is continuously heated by the cyclic reciprocating, and the temperature of the pump body is in a changing state due to the excessive heat and the heat loss.
In order to enable the temperature of the pump body to reach an effect of tending to a steady state, in the embodiment, the temperature is raised proportionally through a temperature control algorithm, and the variation of the temperature is ensured to be small enough through an integral control error, so that the pump body can reach a relatively stable temperature after temperature adjustment. Specifically, assuming that the target temperature of the pump body is T, and the current temperature of the pump body is Tn, the current temperature is:
wherein e is the temperature difference, i.e. e is the difference between the target temperature and the current temperature; kp is a proportional coefficient, Kp/ki is an integral coefficient, Kp and ki take any value between 0 and 1, for example Kp takes 0.4 and ki takes 0.1.
The present invention also provides a storage medium, wherein the storage medium stores a computer program which can be executed for implementing the high precision pipetting method as described in the present invention.
In summary, the present invention discloses an adaptive air-type liquid-displacement pump, which includes a pump body, wherein the pump body has an inner cavity, a piston assembly is slidably disposed in the inner cavity, an air section is disposed between the piston assembly and the bottom of the inner cavity, and the adaptive air-type liquid-displacement pump further includes: the heating device is arranged on the pump body and is used for heating the pump body when the temperature of the liquid to be pipetted is higher than that of the pump body, so that the temperature of the air in the air section is equal to that of the liquid to be pipetted; the refrigerating device is arranged on the pump body and used for refrigerating the pump body when the temperature of the liquid to be transferred is lower than that of the pump body, so that the air temperature in the air section is equal to that of the liquid to be transferred. According to the invention, the heating device and the refrigerating device are arranged on the pump body, and when the temperature of the liquid to be pipetted is not equal to the temperature of the air in the air section in the pump body, the air temperature in the air section is adjusted through the heating device or the refrigerating device until the temperature of the air in the air section is equal to the temperature of the liquid to be pipetted, so that the problem of inaccurate pipetting amount caused by the change of the volume of the air section after the piston assembly moves to a preset position for pipetting during pipetting is avoided.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. An adaptive air-based fluid displacement pump, comprising a pump body having an inner chamber therein, a piston assembly slidably disposed in the inner chamber, and an air section between the piston assembly and the bottom of the inner chamber, wherein the adaptive air-based fluid displacement pump further comprises:
the heating device is arranged on the pump body and is used for heating the pump body when the temperature of the liquid to be pipetted is higher than that of the pump body, so that the temperature of the air in the air section is equal to that of the liquid to be pipetted;
the refrigerating device is arranged on the pump body and used for refrigerating the pump body when the temperature of the liquid to be transferred is lower than that of the pump body, so that the air temperature in the air section is equal to that of the liquid to be transferred.
2. The adaptive air-based pipette of claim 1, wherein the heating device is an electro-thermal film.
3. The adaptive air-based ambulatory pump of claim 1 wherein the refrigeration unit comprises:
the semiconductor refrigerating piece is connected with the pump body;
the moisture insulation piece is connected with one side, back to the pump body, of the semiconductor refrigeration sheet; and
and the heat dissipation block is connected with one side of the moisture insulation piece back to the semiconductor refrigeration piece.
4. The adaptive air-based liquid displacement pump according to claim 3, wherein a bolt is connected to the heat dissipation block, one end of the bolt, which is away from the heat dissipation block, is connected to the pump body, and a heat insulation block is sleeved on a screw of the bolt.
5. The adaptive air-based ambulatory pump of claim 1 further comprising a control assembly, said control assembly being connected to said piston assembly, said heating device, and said cooling device, respectively;
an environment temperature sensor is arranged on one side, back to the pump body, of the control assembly and is connected with the control assembly;
the pump body is provided with a pump body temperature sensor, and the pump body temperature sensor is connected with the control assembly.
6. The adaptive air-based displacement pump of claim 1, wherein the piston assembly comprises:
the motor is connected with the pump body;
the lead screw is connected with the motor;
the piston is connected to one end of the lead screw and is arranged in the inner cavity in a sliding mode;
the sliding block is connected with one end, far away from the piston, of the lead screw; and
the guide rod is parallel to the lead screw, and the sliding block is connected with the guide rod in a sliding mode.
7. A high precision pipetting method based on the adaptive air pipetting pump of claims 1-6, the method comprising:
respectively acquiring the temperature of liquid to be moved and the ambient temperature;
when the temperature of the liquid to be pipetted is higher than the ambient temperature, controlling the heating device to heat the pump body to be equal to the temperature of the liquid to be pipetted, and when the temperature of the liquid to be pipetted is lower than the ambient temperature, controlling the refrigerating device to cool the pump body to be equal to the temperature of the liquid to be pipetted;
and controlling the pump body to perform pipetting.
8. The high-precision pipetting method according to claim 7, wherein the controlling the heating device to heat the pump body to a temperature equal to that of the liquid to be pipetted when the temperature of the liquid to be pipetted is higher than the ambient temperature comprises:
when the temperature of the liquid to be transferred is higher than the environmental temperature, controlling a heating device to heat the pump body, and collecting the temperature of the pump body at intervals of preset interval time;
and when the weighted average value of the temperature values of the pump body acquired five times continuously is greater than the temperature of the liquid to be moved, controlling the heating device to stop heating.
9. The high-precision pipetting method according to claim 7, wherein the controlling the cooling device to cool the pump body to be equal to the temperature of the liquid to be pipetted when the temperature of the liquid to be pipetted is lower than the ambient temperature comprises:
when the temperature of the liquid to be moved is lower than the environmental temperature, controlling a refrigerating device to refrigerate the pump body, and collecting the temperature of the pump body at intervals of preset interval time;
and when the weighted average value of the temperature values of the pump body acquired five times continuously is smaller than the temperature of the liquid to be moved, controlling the refrigerating device to stop refrigerating.
10. A storage medium, characterized in that the storage medium stores a computer program executable for implementing the control method of a plunger pump according to any one of claims 7-9.
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| CN202210437060.4A CN114753985A (en) | 2022-04-22 | 2022-04-22 | Self-adaptive air type liquid transfer pump, high-precision liquid transfer method and storage medium |
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| CN202210437060.4A CN114753985A (en) | 2022-04-22 | 2022-04-22 | Self-adaptive air type liquid transfer pump, high-precision liquid transfer method and storage medium |
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