CN114716283A - Preparation method of organic fertilizer for ecological restoration - Google Patents
Preparation method of organic fertilizer for ecological restoration Download PDFInfo
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- CN114716283A CN114716283A CN202210359576.1A CN202210359576A CN114716283A CN 114716283 A CN114716283 A CN 114716283A CN 202210359576 A CN202210359576 A CN 202210359576A CN 114716283 A CN114716283 A CN 114716283A
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 56
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 53
- 239000010457 zeolite Substances 0.000 claims abstract description 53
- 241000894006 Bacteria Species 0.000 claims abstract description 41
- 239000000843 powder Substances 0.000 claims abstract description 32
- 239000010908 plant waste Substances 0.000 claims abstract description 30
- 239000000203 mixture Substances 0.000 claims abstract description 26
- 239000002245 particle Substances 0.000 claims abstract description 21
- 239000003337 fertilizer Substances 0.000 claims abstract description 18
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 238000012258 culturing Methods 0.000 claims abstract description 12
- 238000000227 grinding Methods 0.000 claims abstract description 8
- 238000002791 soaking Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 20
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 20
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 16
- 241000589516 Pseudomonas Species 0.000 claims description 16
- 229910052751 metal Inorganic materials 0.000 claims description 16
- 239000002184 metal Substances 0.000 claims description 16
- 150000003839 salts Chemical class 0.000 claims description 16
- 241000233866 Fungi Species 0.000 claims description 15
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 10
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 10
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 10
- 239000001110 calcium chloride Substances 0.000 claims description 10
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 10
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 10
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 10
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 239000011780 sodium chloride Substances 0.000 claims description 10
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 10
- 229960001763 zinc sulfate Drugs 0.000 claims description 10
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 10
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 241000589774 Pseudomonas sp. Species 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 241000235342 Saccharomycetes Species 0.000 claims description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 3
- 229930091371 Fructose Natural products 0.000 claims description 3
- 239000005715 Fructose Substances 0.000 claims description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 239000002689 soil Substances 0.000 description 11
- 239000003344 environmental pollutant Substances 0.000 description 9
- 231100000719 pollutant Toxicity 0.000 description 9
- 229910001385 heavy metal Inorganic materials 0.000 description 6
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 238000005067 remediation Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 101100369068 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TDA1 gene Proteins 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003900 soil pollution Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 240000006186 Krugiodendron ferreum Species 0.000 description 1
- 235000005132 Krugiodendron ferreum Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
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- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Soil Sciences (AREA)
- Molecular Biology (AREA)
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Abstract
The invention discloses a preparation method of an organic fertilizer for ecological restoration, which is characterized by comprising the following steps: step 1, grinding blocky zeolite into particles with different sizes, and soaking the particles into a mixed sugar solution; step 2, mixing the treated zeolite powder and the bioremediation bacteria uniformly, and then culturing in a dark place; step 3, stacking the crop waste residues in a container with an opened upper layer; step 4, keeping low-temperature culture in the container for several days, then raising the temperature to high temperature and then culturing for several days; and 5, mixing the bioremediation bacteria/zeolite powder mixture into the crop waste residue fertilizer to obtain the organic fertilizer for ecological restoration. The invention prepares an organic fertilizer with excellent ecological restoration effect.
Description
Technical Field
The invention relates to the field of organic fertilizers, and in particular relates to a preparation method of an organic fertilizer for ecological restoration.
Background
Soil is an essential important resource and material foundation for production, life and other aspects of the human society. Currently, China is in a stage of accelerated development and progress of industrialization and urbanization, various pollutants enter soil, so that the situation of soil pollution is more severe, and the current situation of agricultural environment and the problem of weight safety of agricultural products need to be improved urgently.
In recent years, most of research focuses mainly on environmental behaviors of single pollutants, but in reality, the environmental behaviors are often complex pollutants generated by coexistence and interaction of multiple pollutants, which means that environmental pollution tends to be diversified and complicated. Therefore, the composite pollution composed of various pollutants is gradually developed as a research focus in the field of soil environment. Among them, heavy metals and antibiotics are becoming more and more types of pollutants in the soil system of our country because of their large use and discharge.
Because of a plurality of soil pollution sources including industrial pollution, domestic garbage, sewage irrigation, atmospheric pollutant sedimentation, mineral exploitation, pesticides and the like, the remediation of the polluted soil faces an important challenge that a specific remediation scheme can be formulated only according to the specific pollution condition of the soil, but the polluted soil has different natural environments and different pollution sources, so that the remediation of the soil is slow in development. In addition, the repair cost is high, secondary pollution sources are generated, the use conditions are strict, and the method is also an important factor for limiting the rapid development of soil repair.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a preparation method of an organic fertilizer for ecological restoration.
The purpose of the invention is realized by adopting the following technical scheme:
a preparation method of an organic fertilizer for ecological restoration comprises the following steps:
step 1, grinding blocky zeolite into particles with different sizes, soaking the particles into a mixed sugar solution, and filtering out solids to obtain treated zeolite powder;
step 2, uniformly mixing the treated zeolite powder with the bioremediation bacteria, and then culturing in a shading mode to obtain a bioremediation bacteria/zeolite powder mixture;
step 3, stacking the crop waste residues in a container with an opened upper layer, pouring a small amount of water, placing in a shading mode, and inserting a temperature sensor into 1/3-1/2 parts of the mixture stacked in the container;
step 4, keeping low-temperature culture in the container for several days, then raising the temperature to high temperature, and then culturing for several days to obtain the crop waste residue fertilizer;
and 5, mixing the bioremediation bacteria/zeolite powder mixture into the crop waste residue fertilizer, repeatedly turning until the mixture is uniformly mixed, carrying out closed shading treatment for a period of time, and then carrying out drying treatment to obtain the organic fertilizer for ecological restoration.
Preferably, in step 1, the bulk zeolite is ground to have a particle size interval of: the first interval is 500-1000 μm, the second interval is 300-500 μm, the third interval is 100-300 μm, and the fourth interval is 20-100 μm; the weight ratio of the first interval to the second interval to the third interval to the fourth interval is 4-6: 1-3: 0.5-1: 0.1-0.5.
Preferably, in the step 1, the mixed sugar solution comprises the components of mixed sugar, metal salt and water, and the weight ratio of the mixed sugar to the metal salt to the water is 7.5-25.5: 0.8-1.5: 100.
Preferably, in the step 1, the weight ratio of the zeolite to the mixed sugar solution is 1: 10-20.
Preferably, the mixed sugar comprises at least two of glucose, fructose, maltose, sucrose, starch.
Preferably, the metal salt comprises sodium chloride, calcium chloride, magnesium chloride, zinc sulfate and dipotassium hydrogen phosphate, and the weight ratio of the sodium chloride to the calcium chloride to the magnesium chloride to the zinc sulfate to the dipotassium hydrogen phosphate is 0.22-0.46: 0.15-0.27: 0.19-0: 32: 0.005-0.012: 0.64-0.87.
Preferably, in the step 2, the bioremediation bacteria comprise bacillus, white rot fungi, pseudomonas, lactobacillus acidophilus and yeast; the weight ratio of the bacillus to the white-rot fungi to the pseudomonas to the lactobacillus acidophilus to the saccharomycetes is 2-4: 3-6: 0.5-1.2: 0.06-0.16: 0.4-0.7.
Preferably, in the step 2, the culture time of the bioremediation bacteria is 2-4 days, and the weight ratio of the bioremediation bacteria to the treated zeolite powder is 0.01-0.1: 10.
Preferably, the Bacillus is Bacillus sp (Z-y3) and the pseudomonas is pseudomonas sp.
Preferably, in the step 3, after the water is sprayed, the water content of the crop waste residue is 45-50%.
Preferably, in the step 3, the crop waste residue is obtained by grinding waste materials of rice straws, wheat straws and corn straws.
Preferably, in the step 4, the low temperature is 25-30 ℃, the low temperature culture days are 10-15 days, the high temperature is 55-60 ℃, and the high temperature culture days are 2-3 days.
Preferably, in the step 5, the weight ratio of the bioremediation bacteria/zeolite powder mixture to the crop waste residue fertilizer is 1: 6-10.
Preferably, in the step 5, the time of the closed light shielding treatment is 2 to 5 days.
The invention has the beneficial effects that:
according to the invention, the zeolite with different particle sizes is soaked in the mixed sugar solution, and the zeolite with different particle sizes has adsorption effects with different capabilities, so that zeolite particles with the mixed sugar and metal salt adsorbed thereon are obtained, and then bioremediation bacteria are added for culture, so that the bioremediation bacteria can be fixed on the zeolite particles, and then the zeolite particles are combined with the composted crop waste residues, so that the organic fertilizer with excellent ecological remediation effect is finally prepared.
The combination of various fungi selected by the invention has a good ecological restoration effect, and can restore various pollutants in the ecological environment, such as refractory plastics, heavy metals, refractory compounds and the like. The bacillus can have a good removal effect on heavy metal ions in the heavy metal polluted soil; the pseudomonas is widely distributed in nature, is a main bacterial group with biological control function around plant rhizosphere soil, has a unique function, and can biologically convert oil-based plastics into completely biodegradable plastics, so that other bacteria are matched to complete the degradation of the plastics; the white rot fungi are fungi which lead wood to be white rot and can secrete extracellular oxidase to degrade lignin, and in addition, the white rot fungi have broad-spectrum degradation capability on a large number of refractory compounds because the white rot fungi can generate non-specific extracellular enzyme in the secondary metabolic process.
Detailed Description
The invention is further described below with reference to the following examples.
Example 1
A preparation method of an organic fertilizer for ecological restoration comprises the following steps:
step 1, grinding blocky zeolite into particles with different sizes, soaking the particles into a mixed sugar solution, and filtering out solids to obtain treated zeolite powder;
step 2, uniformly mixing the treated zeolite powder with the bioremediation bacteria, and then culturing in a shading mode to obtain a bioremediation bacteria/zeolite powder mixture;
step 3, stacking the crop waste residues in a container with an opened upper layer, pouring a small amount of water, shading, placing, and inserting a temperature sensor into 1/3-1/2 parts of the mixture stacked in the container;
step 4, keeping low-temperature culture in the container for several days, then raising the temperature to high temperature, and then culturing for several days to obtain the crop waste residue fertilizer;
and 5, mixing the bioremediation bacteria/zeolite powder mixture into the crop waste residue fertilizer, repeatedly turning until the mixture is uniformly mixed, carrying out closed shading treatment for a period of time, and then carrying out drying treatment to obtain the organic fertilizer for ecological restoration.
In step 1, the bulk zeolite is ground to have a particle size interval of: the first interval is 500-1000 μm, the second interval is 300-500 μm, the third interval is 100-300 μm, and the fourth interval is 20-100 μm; the weight ratio of the first interval to the second interval to the third interval to the fourth interval is 5:2:0.5: 0.1.
In the step 1, the mixed sugar solution comprises the components of mixed sugar, metal salt and water, and the weight ratio of the mixed sugar to the metal salt to the water is 14.3:1.1: 100.
In the step 1, the weight ratio of the zeolite to the mixed sugar solution is 1: 15.
The mixed sugar comprises glucose and fructose.
The metal salt comprises sodium chloride, calcium chloride, magnesium chloride, zinc sulfate and dipotassium hydrogen phosphate, and the weight ratio of the sodium chloride to the calcium chloride to the magnesium chloride to the zinc sulfate to the dipotassium hydrogen phosphate is 0.31:0.19:0.24:0.007: 0.74.
In the step 2, the bioremediation bacteria comprise bacillus, white rot fungi, pseudomonas, lactobacillus acidophilus and saccharomycetes; the weight ratio of the bacillus to the white-rot fungi to the pseudomonas to the lactobacillus acidophilus to the yeast is 3:5:0.9:0.12: 0.6.
In the step 2, the culture time of the bioremediation bacteria is 2-4 days, and the weight ratio of the bioremediation bacteria to the treated zeolite powder is 0.05: 10.
The Bacillus is Bacillus sp (Z-y3), and the pseudomonas is pseudomonas sp.TDA1.
In the step 3, after the water is sprayed, the water content of the crop waste residue is 50%.
In the step 4, the low temperature is 25 ℃, the low temperature culture days are 15 days, the high temperature is 55 ℃, and the high temperature culture days are 2 days.
In the step 5, the weight ratio of the bioremediation bacterium/zeolite powder mixture to the crop waste residue fertilizer is 1: 8.
In the step 5, the time of the closed shading treatment is 3 days.
Example 2
A preparation method of an organic fertilizer for ecological restoration comprises the following steps:
step 1, grinding blocky zeolite into particles with different sizes, soaking the particles into a mixed sugar solution, and filtering out solids to obtain treated zeolite powder;
step 2, uniformly mixing the treated zeolite powder with the bioremediation bacteria, and then culturing in a shading mode to obtain a bioremediation bacteria/zeolite powder mixture;
step 3, stacking the crop waste residues in a container with an opened upper layer, pouring a small amount of water, placing in a shading mode, and inserting a temperature sensor into 1/3-1/2 parts of the mixture stacked in the container;
step 4, keeping low-temperature culture in the container for several days, then raising the temperature to high temperature, and then culturing for several days to obtain the crop waste residue fertilizer;
and 5, mixing the bioremediation bacteria/zeolite powder mixture into the crop waste residue fertilizer, repeatedly turning over until the mixture is uniformly mixed, carrying out closed shading treatment for a period of time, and then carrying out drying treatment to obtain the organic fertilizer for ecological restoration.
In step 1, the bulk zeolite is ground to have a particle size interval of: the first interval is 500-1000 μm, the second interval is 300-500 μm, the third interval is 100-300 μm, and the fourth interval is 20-100 μm; the weight ratio of the first interval to the second interval to the third interval to the fourth interval is 4:1:0.5: 0.1.
In the step 1, the mixed sugar solution comprises the components of mixed sugar, metal salt and water, and the weight ratio of the mixed sugar to the metal salt to the water is 7.5:0.8: 100.
In the step 1, the weight ratio of the zeolite to the mixed sugar solution is 1: 10.
The mixed sugar comprises glucose and maltose.
The metal salt comprises sodium chloride, calcium chloride, magnesium chloride, zinc sulfate and dipotassium hydrogen phosphate, and the weight ratio of the sodium chloride to the calcium chloride to the magnesium chloride to the zinc sulfate to the dipotassium hydrogen phosphate is 0.22:0.15:0.19:0.005: 0.64.
In the step 2, the bioremediation bacteria comprise bacillus, white rot fungi, pseudomonas, lactobacillus acidophilus and saccharomycetes; the weight ratio of the bacillus to the white-rot fungi to the pseudomonas to the lactobacillus acidophilus to the yeast is 2:3:0.5:0.06: 0.4.
In the step 2, the culture time of the bioremediation bacteria is 2 days, and the weight ratio of the bioremediation bacteria to the treated zeolite powder is 0.01: 10.
The Bacillus is Bacillus sp. (Z-y3), and the pseudomonas is pseudomonas sp.
In the step 3, after the water is sprayed, the water content of the crop waste residue is 45%.
In the step 4, the low temperature is 25 ℃, the low temperature culture days are 10 days, the high temperature is 55 ℃, and the high temperature culture days are 2 days.
In the step 5, the weight ratio of the bioremediation bacteria/zeolite powder mixture to the crop waste residue fertilizer is 1: 6.
In the step 5, the time of the closed shading treatment is 2 days.
Example 3
A preparation method of an organic fertilizer for ecological restoration comprises the following steps:
step 1, grinding blocky zeolite into particles with different sizes, soaking the particles into a mixed sugar solution, and filtering out solids to obtain treated zeolite powder;
step 2, uniformly mixing the treated zeolite powder with the bioremediation bacteria, and then culturing in a shading mode to obtain a bioremediation bacteria/zeolite powder mixture;
step 3, stacking the crop waste residues in a container with an opened upper layer, pouring a small amount of water, placing in a shading mode, and inserting a temperature sensor into 1/3-1/2 parts of the mixture stacked in the container;
step 4, keeping low-temperature culture in the container for several days, then raising the temperature to high temperature, and then culturing for several days to obtain the crop waste residue fertilizer;
and 5, mixing the bioremediation bacteria/zeolite powder mixture into the crop waste residue fertilizer, repeatedly turning until the mixture is uniformly mixed, carrying out closed shading treatment for a period of time, and then carrying out drying treatment to obtain the organic fertilizer for ecological restoration.
In step 1, the bulk zeolite is ground to have a particle size interval of: the first interval is 500-1000 μm, the second interval is 300-500 μm, the third interval is 100-300 μm, and the fourth interval is 20-100 μm; the weight ratio of the first interval to the second interval to the third interval to the fourth interval is 6:3:1: 0.5.
In the step 1, the mixed sugar solution comprises the components of mixed sugar, metal salt and water, and the weight ratio of the mixed sugar to the metal salt to the water is 25.5:1.5: 100.
In the step 1, the weight ratio of the zeolite to the mixed sugar solution is 1: 20.
The mixed sugar comprises glucose and sucrose.
The metal salt comprises sodium chloride, calcium chloride, magnesium chloride, zinc sulfate and dipotassium hydrogen phosphate, and the weight ratio of the sodium chloride to the calcium chloride to the magnesium chloride to the zinc sulfate to the dipotassium hydrogen phosphate is 0.46:0.27:0:32:0.012: 0.87.
In the step 2, the bioremediation bacteria comprise bacillus, white rot fungi, pseudomonas, lactobacillus acidophilus and saccharomycetes; the weight ratio of the bacillus to the white-rot fungi to the pseudomonas to the lactobacillus acidophilus to the yeast is 4:6:1.2:0.16: 0.7.
In the step 2, the culture time of the bioremediation bacteria is 4 days, and the weight ratio of the bioremediation bacteria to the treated zeolite powder is 0.1: 10.
The Bacillus is Bacillus sp (Z-y3), and the pseudomonas is pseudomonas sp.TDA1.
In the step 3, after the water is sprayed, the water content of the crop waste residue is 50%.
In the step 4, the low temperature is 30 ℃, the low temperature culture days are 15 days, the high temperature is 60 ℃, and the high temperature culture days are 3 days.
In the step 5, the weight ratio of the bioremediation bacteria/zeolite powder mixture to the crop waste residue fertilizer is 1: 10.
In the step 5, the time of the closed shading treatment is 5 days.
Comparative example
A preparation method of an organic fertilizer for ecological restoration comprises the following steps:
grinding the blocky zeolite into particles with different sizes, wherein the particle size interval of the blocky zeolite is as follows: the first interval is 500-1000 μm, the second interval is 300-500 μm, the third interval is 100-300 μm, and the fourth interval is 20-100 μm; the weight ratio of the first interval to the second interval to the third interval to the fourth interval is 6:3:1: 0.5;
and uniformly mixing the crop waste residue fertilizer, the bioremediation bacteria and the ground zeolite to obtain the organic fertilizer.
Examples of the experiments
Selecting a winter wheat field, dividing the winter wheat field into two parts, normally planting wheat, respectively fertilizing 100 kg/mu of the organic fertilizer for ecological restoration in the embodiment 1 and the comparative example, and after 8 months of fertilization, detecting the heavy metal content, the organic phosphorus pollutant content and the yield increase. The results show that: by reference to the comparative example, the yield of example 1 was increased by about 5%, the heavy metal content was reduced by about 10%, and the organophosphorus contaminant content was reduced by about 6%.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. A preparation method of an organic fertilizer for ecological restoration is characterized by comprising the following steps:
step 1, grinding blocky zeolite into particles with different sizes, soaking the particles into a mixed sugar solution, and filtering out solids to obtain treated zeolite powder;
step 2, uniformly mixing the treated zeolite powder with the bioremediation bacteria, and then culturing in a shading mode to obtain a bioremediation bacteria/zeolite powder mixture;
step 3, stacking the crop waste residues in a container with an opened upper layer, pouring a small amount of water, placing in a shading mode, and inserting a temperature sensor into 1/3-1/2 parts of the mixture stacked in the container;
step 4, keeping low-temperature culture in the container for several days, then raising the temperature to high temperature, and then culturing for several days to obtain the crop waste residue fertilizer;
and 5, mixing the bioremediation bacteria/zeolite powder mixture into the crop waste residue fertilizer, repeatedly turning until the mixture is uniformly mixed, carrying out closed shading treatment for a period of time, and then carrying out drying treatment to obtain the organic fertilizer for ecological restoration.
2. The method as claimed in claim 1, wherein in the step 1, the zeolite is ground into the following particle size ranges: the first interval is 500-1000 μm, the second interval is 300-500 μm, the third interval is 100-300 μm, and the fourth interval is 20-100 μm; the weight ratio of the first interval to the second interval to the third interval to the fourth interval is 4-6: 1-3: 0.5-1: 0.1-0.5.
3. The method for preparing an organic fertilizer for ecological restoration according to claim 1, wherein in the step 1, the mixed sugar solution comprises the components of mixed sugar, metal salt and water, and the weight ratio of the mixed sugar to the metal salt to the water is 7.5-25.5: 0.8-1.5: 100; the weight ratio of the zeolite to the mixed sugar solution is 1: 10-20.
4. The method as claimed in claim 1, wherein the mixed sugar comprises at least two of glucose, fructose, maltose, sucrose and starch; the metal salt comprises sodium chloride, calcium chloride, magnesium chloride, zinc sulfate and dipotassium hydrogen phosphate, and the weight ratio of the sodium chloride to the calcium chloride to the magnesium chloride to the zinc sulfate to the dipotassium hydrogen phosphate is 0.22-0.46: 0.15-0.27: 0.19-0: 32: 0.005-0.012: 0.64-0.87.
5. The method as claimed in claim 1, wherein in step 2, the bioremediation bacteria include bacillus, white rot fungi, pseudomonas, lactobacillus acidophilus and yeast; the weight ratio of the bacillus to the white-rot fungi to the pseudomonas to the lactobacillus acidophilus to the saccharomycetes is 2-4: 3-6: 0.5-1.2: 0.06-0.16: 0.4-0.7.
6. The method for preparing an organic fertilizer for ecological restoration according to claim 1, wherein in the step 2, the culture time of the bioremediation bacteria is 2-4 days, and the weight ratio of the bioremediation bacteria to the treated zeolite powder is 0.01-0.1: 10.
7. The method for preparing an organic fertilizer for ecological restoration according to claim 1, wherein the Bacillus is Bacillus sp (Z-y3), and the pseudomonas is pseudomonas sp.tda 1.
8. The method for preparing an organic fertilizer for ecological restoration according to claim 1, wherein in the step 3, the water content of the crop waste residue is 45-50% after the water is poured in.
9. The method for preparing the organic fertilizer for ecological restoration according to claim 1, wherein in the step 4, the low temperature is 25 to 30 ℃, the low temperature culture days are 10 to 15 days, the high temperature is 55 to 60 ℃, and the high temperature culture days are 2 to 3 days.
10. The method for preparing an organic fertilizer for ecological restoration according to claim 1, wherein in the step 5, the weight ratio of the bioremediation bacteria/zeolite powder mixture to the crop waste residue fertilizer is 1: 6-10; the time of the closed shading treatment is 2-5 days.
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