CN114702141A - Application of aniline degrading bacterium providencia rettgeri in dye decoloration - Google Patents
Application of aniline degrading bacterium providencia rettgeri in dye decoloration Download PDFInfo
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- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 title claims abstract description 121
- 230000000593 degrading effect Effects 0.000 title claims abstract description 22
- 241000588777 Providencia rettgeri Species 0.000 title claims abstract description 21
- 238000004042 decolorization Methods 0.000 claims abstract description 47
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 claims abstract description 45
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 claims abstract description 38
- 239000000975 dye Substances 0.000 claims abstract description 38
- 230000015556 catabolic process Effects 0.000 claims abstract description 34
- 238000006731 degradation reaction Methods 0.000 claims abstract description 34
- 239000001963 growth medium Substances 0.000 claims abstract description 27
- STZCRXQWRGQSJD-GEEYTBSJSA-M methyl orange Chemical compound [Na+].C1=CC(N(C)C)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 STZCRXQWRGQSJD-GEEYTBSJSA-M 0.000 claims abstract description 14
- 229940012189 methyl orange Drugs 0.000 claims abstract description 14
- 239000000987 azo dye Substances 0.000 claims abstract description 9
- 238000011218 seed culture Methods 0.000 claims abstract description 6
- 239000001000 anthraquinone dye Substances 0.000 claims abstract description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 56
- 239000011780 sodium chloride Substances 0.000 claims description 28
- 239000001888 Peptone Substances 0.000 claims description 27
- 108010080698 Peptones Proteins 0.000 claims description 27
- 229940041514 candida albicans extract Drugs 0.000 claims description 27
- 235000019319 peptone Nutrition 0.000 claims description 27
- 239000012138 yeast extract Substances 0.000 claims description 27
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 15
- 230000000721 bacterilogical effect Effects 0.000 claims description 15
- 239000008103 glucose Substances 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 6
- 239000001044 red dye Substances 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 13
- 238000004043 dyeing Methods 0.000 abstract description 9
- 239000002351 wastewater Substances 0.000 abstract description 9
- 239000007788 liquid Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 25
- 238000000034 method Methods 0.000 description 14
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 13
- 239000007836 KH2PO4 Substances 0.000 description 12
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 12
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 12
- 229910052564 epsomite Inorganic materials 0.000 description 12
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 12
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 12
- 238000002835 absorbance Methods 0.000 description 10
- 241001052560 Thallis Species 0.000 description 8
- 238000005070 sampling Methods 0.000 description 8
- 150000004982 aromatic amines Chemical class 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 230000003247 decreasing effect Effects 0.000 description 4
- -1 aniline compound Chemical class 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
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- 238000002474 experimental method Methods 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- C02F3/30—Aerobic and anaerobic processes
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Abstract
The invention discloses an application of a strain of aniline degrading bacterium providencia rettgeri in dye decolorization, wherein 15-7 seeds of providencia rettgeri are inoculated into a seed culture medium for culture to obtain a seed solution. The seed liquid is inoculated into a dye degradation culture medium for culture, so that the dye can be decolorized and aniline can be degraded. The aniline degrading bacteria Provedorsia rapae Y15-7 can aerobically degrade azo dye Congo red and methyl orange, anaerobically degrade anthraquinone dye active brilliant blue KN-R, have broad spectrum of decolorization, and have the functions of decolorization and aniline removal at the same time, and have wide application prospect in the treatment of printing and dyeing wastewater containing high chroma and aniline.
Description
Technical Field
The invention relates to an application of an aniline degrading bacterium providencia rettgeri in dye decolorization, belonging to the technical field of environmental microorganisms and wastewater treatment.
Background
The printing and dyeing wastewater has the characteristics of high chromaticity, complex components, large discharge amount and the like, and is a kind of industrial wastewater which is difficult to treat. Of these, the benzidine-type azo dyes are the most common dyes. As the azo dye is produced by taking the aniline compound as a raw material, the final product contains a certain amount of the aniline compound, and the aniline compound enters wastewater when the dye is applied. The dominant bacteria gradually become the development trend of the printing and dyeing wastewater biological treatment technology by strengthening the decoloration and pollutant degradation of the printing and dyeing wastewater. However, the currently found dominant bacteria are often single in function, and need to be added with different functional microorganisms or mixed bacteria of different functional microorganisms to enhance the biochemical treatment effect of the printing and dyeing wastewater (dunghai, zhangyewei, yangyao flying, etc., research on the decolorization and pollutant degradation of the printing and dyeing wastewater enhanced by the dominant bacteria [ J ], resource saving and environmental protection, 2014(10):53-54, cai, degradation and decolorization of the mixed bacteria fermentation system of aniline in the printing and dyeing wastewater [ J ], printing and dyeing, 2018(17): 39-43.), and the currently found dye degrading bacteria are generally anaerobic degrading bacteria, and the intermediate product for degrading azo dyes is toxic aromatic amine. And few research reports are reported on the aerobic degradation bacteria of the dye, particularly on the multifunctional bacteria which can decolorize and remove aniline simultaneously.
Meanwhile, no research report on the degradation of the dye by providencia rettgeri is found at present.
Disclosure of Invention
The technical problem to be solved by the invention is to provide an application of an aniline degrading bacterium in degrading and decoloring dyes and simultaneously degrading aniline, wherein the strain is named as Providencia rettgeri Y15-7, is preserved in China center for type culture collection in 8 and 26 months in 2019, and has a preservation number of CCTCC NO: m2019664, the preservation unit address is Wuhan city, Hubei province No. 299 in eight branches.
The aniline degrading bacteria providencia rettgeri Y15-7 can degrade azo dyes such as Congo red, methyl orange and anthraquinone dyes such as reactive brilliant blue KN-R.
The method for degrading the dye by using the aniline degrading bacteria providencia rettgeri Y15-7 is used for inoculating the providencia rettgeri 15-7 seeds into a degradation culture medium for culturing.
Seed medium (g/L) for culturing the aniline-degrading bacterium providencia rettgeri Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
The medium (g/L) used for studying the degrading ability of the aniline degrading bacterium Providence reygeri (Providencia rettgeri) Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0 percent of Congo red (or reactive brilliant blue KN-R0.01-0.04 percent) and 0.1-0.8 percent of pH value of 4.0-9.0.
The culture temperature of the seed culture medium is 30 ℃, the rotating speed of a shaking table is 160 r/min, and the culture time is 12 h; the culture temperature of the degradation culture medium is 30 ℃, the rotating speed of a shaking table is 0-160 r/min, and the culture time is 48 h.
The optimal degradation condition of the Congo red dye is aerobic culture at the concentration of 200-: the concentration is 20mg/L, pH 6-7, and the anaerobic culture is carried out in a standing way.
The culture medium for decoloring the dye and simultaneously degrading aniline comprises the following components: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4 & 7H2O 0.2.2, KH2PO40.5, K2HPO41.5, KNO 31.0, Congo red 0.4 (or methyl orange 0.02 or reactive brilliant blue KN-R0.02), aniline 0.2 or 0.4, pH 6.85
The method for decoloring the dye and degrading the aniline comprises the step of inoculating the providencia rettgeri 15-7 seeds into a culture medium containing the dye and the aniline to culture for 48 hours.
The dye Congo red and aniline are cultured aerobically at 160R/min, the methyl orange and aniline are cultured aerobically at 80R/min, and the active brilliant blue KN-R and aniline are cultured anaerobically under standing.
The invention has the advantages that: the aniline degrading bacteria Provesia rapae Y15-7 can aerobically degrade azo dye Congo red and methyl orange, anaerobically degrade anthraquinone dye active brilliant blue KN-R, have broad-spectrum decolorization, have the functions of decolorization and aniline removal, and have wide application prospect in treatment of printing and dyeing wastewater containing high chroma and aniline.
Drawings
FIG. 1 shows the decolorization of Congo red by strain Y15-7 under different rotation speed conditions.
FIG. 2 shows the decolorization of reactive brilliant blue by strain Y15-7 at different rotation speeds.
FIG. 3 shows the decolorization of Congo red by strain Y15-7 under different pH conditions.
FIG. 4 shows the decolorization of reactive brilliant blue KN-R by strain Y15-7 under different pH conditions.
FIG. 5 shows the decolorization of strain Y15-7 against Congo red at different concentrations.
FIG. 6 shows the decolorization of the strain Y15-7 against different concentrations of reactive brilliant blue KN-R.
FIG. 7 shows the detection of aromatic amine product in the process of decolorizing Congo red by strain Y15-7.
FIG. 8 shows that strain Y15-7 degrades aniline and Congo red simultaneously.
FIG. 9 shows that strain Y15-7 degrades aniline and reactive brilliant blue KN-R simultaneously.
FIG. 10 shows that strain Y15-7 degrades aniline and methyl orange simultaneously.
Detailed Description
Example 1 decolorization of Congo Red by Strain Y15-7 at different rotation speeds
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10，MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, Congo Red 0.4, pH 6.85.
Inoculating the strain Y15-7 stored on the plate into a seed culture medium, and culturing at 30 ℃ and 160 r/min for 12 h to obtain a seed solution; inoculating the seed solution into Congo red degradation culture medium according to 1% inoculation amount, culturing at 30 deg.C and different table rotation speeds (0, 40, 80, 160 r/min) for 48h, sampling to determine solution absorbance, and calculating decolorization rate, with the result shown in FIG. 1. As can be seen from figure 1, the strain Y15-7 has a certain decolorizing effect on Congo red at different rotating speeds, and the decolorizing effect is the best under the aerobic condition of 160 r/min, and the decolorizing rate is as high as 93.8%.
Example 2 decolorization of reactive Brilliant blue KN-R by Strain Y15-7 at different spin speeds
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, reactive brilliant blue KN-R0.02, pH 6.85.
According to the method of example 1, the strain Y15-7 was inoculated into a reactive brilliant blue KN-R degradation medium, cultured at 30 ℃ for 48 hours at different shaker rotation speeds (0, 40, 80, 160R/min), sampled to measure the absorbance of the solution, and the decolorization rate was calculated, and the results are shown in FIG. 2. As can be seen from figure 2, the decolorization rate of the strain Y15-7 to the reactive brilliant blue KN-R gradually decreases with the increase of the rotating speed, and the decolorization effect under the anaerobic condition of 0R/min is the best, and the decolorization rate is 39%.
Example 3 decolorization of Congo Red by Strain Y15-7 under different pH conditions
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium for investigating the degradability of strain Y15-7 (g/L): glucose 2, bacteriological proteinPeptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0 percent, Congo red 0.4 percent and pH value 4.0-9.0.
According to the method of example 1, strain Y15-7 was inoculated into Congo red degradation medium having pH of 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, respectively, and cultured at 30 ℃ and 160 r/min for 48 hours, and the absorbance of the solution was measured by sampling to calculate the decolorization ratio, and the results are shown in FIG. 3. As can be seen from FIG. 3, the strain Y15-7 has a good effect of decolorizing congo red under pH 6.0-9.0, namely under neutral to alkaline conditions, the decolorization rate is between 84-86%, and the decolorization rate under acidic conditions is reduced.
Example 4 decolorization of reactive Brilliant blue KN-R by Strain Y15-7 under different pH conditions
Seed medium (g/L) for culturing strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, reactive brilliant blue KN-R0.02, pH 4.0-9.0.
According to the method of example 1, the strain Y15-7 was inoculated into a reactive Brilliant blue KN-R degradation medium having a pH of 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0, respectively, and cultured at 30 ℃ and 0R/min for 48 hours, and the absorbance of the solution was measured by sampling to calculate the decolorization ratio, and the results are shown in FIG. 4. As can be seen from FIG. 4, strain Y15-7 has a good decolorizing effect on reactive brilliant blue KN-R at pH 6.0-7.0, i.e., neutral condition, with the decolorizing rate stabilized at 40%, and the decolorizing rates decreased under acidic and alkaline conditions.
Example 5 decolorization of Congo Red by Strain Y15-7 at various concentrations
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, Yeast0.5 part of extract, 10 parts of NaCl and MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0 percent, Congo red 0.1-0.8 percent and pH 6.85.
According to the method of example 1, the strain Y15-7 was inoculated into degradation media having Congo red concentrations of 100, 200, 300, 400, 500, 600, 700, and 800 mg/L, respectively, and cultured at 30 ℃ and 160 r/min for 48 hours, and the absorbance of the solution was measured by sampling, and the decolorization ratio was calculated, and the results are shown in FIG. 5. As can be seen from FIG. 5, the decolorization rate is 55% when the concentration of Congo red is lower than 100mg/L, is stabilized between 85% and 87% when the concentration of Congo red reaches 200-400 mg/L, and is decreased when the concentration of Congo red is further increased, but is still maintained at about 70%.
Example 6 decolorization of Strain Y15-7 against different concentrations of reactive Brilliant blue KN-R
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, reactive brilliant blue KN-R0.01-0.04, pH 6.85.
According to the method of example 1, the strain Y15-7 was inoculated into degradation media with active brilliant blue KN-R concentrations of 10, 20, 30 and 40 mg/L, respectively, and cultured at 30 ℃ and 0R/min for 48 hours, and the absorbance of the solution was measured by sampling and the decolorization ratio was calculated, and the results are shown in FIG. 6. As can be seen from FIG. 6, the decolorization rate was 38% when the concentration of the reactive brilliant blue KN-R was low at 10mg/L, 43% when the concentration of the reactive brilliant blue KN-R reached 20mg/L, and decreased when the concentration of the reactive brilliant blue KN-R was further increased.
Example 7 adsorptive decolorization of Congo Red and reactive Brilliant blue KN-R by Strain Y15-7
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
ForMedium for investigating the adsorption Capacity of Strain Y15-7 (g/L): glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO3 1.0，pH 6.85。
According to the method of example 1, the strain Y15-7 is inoculated into a degradation medium without dye, the degradation medium is cultured for 48 hours at 30 ℃ and 160 r/min, the thalli are obtained by centrifugation, then the thalli are suspended in the degradation medium containing 400mg/L congo red, an adsorption experiment is carried out after 20 minutes of inactivation at 120 ℃, and the absorbance of the solution is measured after 48 hours. The result shows that the decolorization rate of the inactivated thalli to Congo red is only 37.8 percent and is far lower than the decolorization rate of the thalli without inactivation which is 87.02 percent, and the bacterial strain mainly plays a role in degrading and decolorizing Congo red.
Similarly, according to the method of example 1, the strain Y15-7 is inoculated into a degradation medium without dye, cultured for 48h at 30 ℃ and 0R/min, centrifuged to obtain thalli, then the thalli are suspended in the degradation medium containing 20mg/L of active brilliant blue KN-R, and after 20 min of inactivation at 120 ℃, an adsorption experiment is carried out, and after 48h, the absorbance of the solution is measured. The result shows that the decolorization rate of the inactivated thalli to the active brilliant blue KN-R is 0.08 percent and is far lower than the decolorization rate of the thalli not inactivated by 41.9 percent, which indicates that the strain has degradation and decolorization effects on the active brilliant blue KN-R.
EXAMPLE 8 detection of aromatic amine product during Congo Red degradation by Strain Y15-7
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, Congo Red 0.4, pH 6.85.
According to the method of example 1, the strain Y15-7 was inoculated into a degradation medium with a Congo red concentration of 400mg/L, cultured at 30 ℃ and 160 r/min, and the aniline content in the solution was measured by sampling at different time points, and the measurement results of the aniline absorbance are shown in FIG. 7. As can be seen from fig. 7, a small amount of aromatic amine was present in the dye sample, which is probably because the reaction was incomplete when the dye was synthesized, resulting in residual aromatic amine remaining. With the progress of decolorization, the absorbance of the aniline substances is increased and then decreased, and finally the aniline substances tend to be stable. The degradation of congo red is accompanied with the generation of aromatic amine, but the strain Y15-7 can remove the aromatic amine while decoloring, thereby solving the problem that the intermediate product of azo dye degradation pollutes the environment.
Example 9 Strain Y15-7 degrades aniline and Congo red simultaneously
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium for investigating the degradability of strain Y15-7 (g/L): glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, congo red 0.4, aniline 0.2, pH 6.85.
According to the method of example 1, the strain Y15-7 was inoculated into a degradation medium containing congo red and aniline at the same time, cultured at 30 ℃ for 48 hours at 160 r/min, and the aniline removal rate and the dye decolorization rate were measured by sampling, and the results are shown in FIG. 8. As can be seen from FIG. 8, the strain Y15-7 can degrade aniline and Congo red simultaneously, and has a Congo red decolorization rate of 74.1% and a p-phenylenediamine removal rate of 64.1%.
EXAMPLE 10 Strain Y15-7 degrades aniline and reactive Brilliant blue KN-R simultaneously
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, reactive brilliant blue KN-R0.02, aniline 0.2 and pH 6.85.
According to the method of example 1, the strain Y15-7 was inoculated into a degradation medium containing both reactive brilliant blue KN-R and aniline, cultured at 30 ℃ for 48 hours at 0R/min, and the aniline removal rate and the dye decolorization rate were measured by sampling, and the results are shown in FIG. 9. As can be seen from FIG. 9, the strain Y15-7 can degrade aniline and reactive brilliant blue KN-R simultaneously, and has a decolorization rate of 38.4% for the reactive brilliant blue KN-R and a removal rate of 50.4% for aniline.
EXAMPLE 11 Strain Y15-7 degrades aniline and methyl orange simultaneously
Seed medium (g/L) for the cultivation of strain Y15-7: NaCl 10, peptone 10, yeast extract 5, pH 7.0.
Culture medium (g/L) for studying the degradability of strain Y15-7: glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4·7H2O 0.2，KH2PO4 0.5，K2HPO4 1.5，KNO31.0, methyl orange 0.02, aniline 0.4, pH 6.85.
According to the method of example 1, strain Y15-7 was inoculated into degradation medium containing both methyl orange and aniline, and cultured at 30 ℃ at 80r/min, and samples were taken at different time points to determine the aniline removal rate and the dye decolorization rate, respectively, and the results are shown in FIG. 10. As can be seen from FIG. 10, the strain Y15-7 can degrade aniline and methyl orange simultaneously, the aniline concentration is reduced from 427.23 mg/L to 42.99 mg/L within 48h, the degradation rate is 89.9%, the methyl orange concentration is reduced from 23.37 mg/L to 13.07 mg/L, and the removal rate is 44.1%.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Sequence listing
<110> college university
<120> application of aniline degrading bacterium providencia rettgeri in dye decoloration
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1407
<212> DNA
<213> Provedasius reynaudii (Providencia rettgeri Y15-7)
<400> 1
gcaagtcgag cggtaacagg ggaagcttgc ttctcgctga cgagcggcgg acgggtgagt 60
aatgtatggg gatctgcccg atagaggggg ataactactg gaaacggtag ctaataccgc 120
ataatctctt aggagcaaag caggggaact tcggtccttg cgctatcgga tgaacccata 180
tgggattagc tagtaggtgg ggtaatggct cacctaggcg acgatcccta gctggtctga 240
gaggatgatc agccacactg ggactgagac acggcccaga ctcctacggg aggcagcagt 300
ggggaatatt gcacaatggg cgcaagcctg atgcagccat gccgcgtgta tgaagaaggc 360
cctagggttg taaagtactt tcagtcggga ggaaggcgtt gatgctaata tcatcaacga 420
ttgacgttac cgacagaaga agcaccggct aactccgtgc cagcagccgc ggtaatacgg 480
agggtgcaag cgttaatcgg aattactggg cgtaaagcgc acgcaggcgg ttgattaagt 540
tagatgtgaa atccccgggc ttaacctggg aatggcatct aagactggtc agctagagtc 600
ttgtagaggg gggtagaatt ccatgtgtag cggtgaaatg cgtagagatg tggaggaata 660
ccggtggcga aggcggcccc ctggacaaag actgacgctc aggtgcgaaa gcgtggggag 720
caaacaggat tagataccct ggtagtccac gctgtaaacg atgtcgattt gaaggttgtt 780
cccttgagga gtggctttcg gagctaacgc gttaaatcga ccgcctgggg agtacggccg 840
caaggttaaa actcaaatga attgacgggg gcccgcacaa gcggtggagc atgtggttta 900
attcgatgca acgcgaagaa ccttacctac tcttgacatc cagagaactt agcagagatg 960
ctttggtgcc ttcgggaact ctgagacagg tgctgcatgg ctgtcgtcag ctcgtgttgt 1020
gaaatgttgg gttaagtccc gcaacgagcg caacccttat cctttgttgc cagcgattcg 1080
gtcgggaact caaaggagac tgccggtgat aaaccggagg aaggtgggga tgacgtcaag 1140
tcatcatggc ccttacgagt agggctacac acgtgctaca atggcgtata caaagagaag 1200
cgacctcgcg agagcaagcg gaactcataa agtacgtcgt agtccggatt ggagtctgca 1260
actcgactcc atgaagtcgg aatcgctagt aatcgtagat cagaatgcta cggtgaatac 1320
gttcccgggc cttgtacaca ccgcccgtca caccatggga gtgggttgca aaagaagtag 1380
gtagcttaac cttcgggagg gcgctta 1407
Claims (4)
1. The application of the aniline degrading bacteria providencia rettgeri in dye decoloration is characterized in that: inoculating the providencia rettgeri 15-7 seeds into a seed culture medium for culture to obtain a seed solution, inoculating the seed solution into a dye degradation culture medium for culture, and realizing the decolorization of the dye, wherein the seed culture medium (g/L) is as follows: NaCl 10, peptone 10, yeast extract 5 and pH 7.0, wherein the culture temperature of the seed culture medium is 30 ℃, the rotation speed of a shaking table is 160R/min, the culture time is 12 hours, the dyes are azo dye congo red and anthraquinone dye reactive brilliant blue KN-R, and the dye degradation culture medium (g/L) is as follows: 0.1-0.8 Congo red, at least one of active brilliant blue KN-R0.01-0.04, glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO 4.7H 2O 0.2.2, KH2PO40.5, K2HPO41.5, KNO 31.0, pH 4.0-9.0, the culture temperature of the degradation culture medium is 30 ℃, the rotation speed of a shaking table is 0-160R/min, and the culture time is 48H.
2. The use of the strain of providencia rettgeri for decolorizing dyes according to claim 1, wherein the strain comprises: the optimal degradation condition of the Congo red dye is aerobic culture at the concentration of 200-: the concentration is 20mg/L, pH 6-7, and the anaerobic culture is carried out in a standing way.
3. The use of the strain of providencia rettgeri for decolorizing dyes according to claim 1, wherein the strain comprises: the dye also comprises azo dyes of Congo red, methyl orange and anthraquinone dye reactive brilliant blue KN-R, aniline can be degraded while dye decoloration is realized, and a culture medium (g/L) containing the dye and the aniline is as follows: 0.4 percent of Congo red, 0.02 percent of methyl orange, at least one of active brilliant blue KN-R0.02, glucose 2, bacteriological peptone 1, 0.5 percent of yeast extract, 10 percent of NaCl, 7 & MgSO 4H 2O 0.2.2, KH2PO40.5, K2HPO41.5, 31.0 percent of KNO, 0.2-0.4 percent of aniline and pH 6.85.
4. The use of the strain of providencia rettgeri for decolorizing dyes according to claim 3, wherein: and inoculating the providencia rettgeri 15-7 seed solution into a culture medium containing dye and aniline, and culturing for 48h to realize the decolorization of the dye and the degradation of the aniline, wherein the dye congo red and the aniline are cultured aerobically at 160R/min, the methyl orange and the aniline are cultured aerobically at 80R/min, and the active brilliant blue KN-R and the aniline are cultured anaerobically at a standing state.
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| CN113403232A (en) * | 2021-07-13 | 2021-09-17 | 重庆邮电大学 | Method for screening phenanthrene polycyclic aromatic hydrocarbon degrading bacteria |
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| CN104245955A (en) * | 2012-03-19 | 2014-12-24 | 文塔纳医疗***公司 | Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria |
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| CN111269861A (en) * | 2020-03-12 | 2020-06-12 | 集美大学 | Providencia rettgeri with aniline degradation and denitrification capabilities and application thereof |
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| US5618726A (en) * | 1991-03-27 | 1997-04-08 | Idaho Research Foundation, Inc. | Biodegradable azo dyes |
| CN104245955A (en) * | 2012-03-19 | 2014-12-24 | 文塔纳医疗***公司 | Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria |
| CN103289940A (en) * | 2013-06-19 | 2013-09-11 | 重庆大学 | Providencia rettgeri strain and application thereof |
| CN108395002A (en) * | 2017-02-08 | 2018-08-14 | 广州中国科学院先进技术研究所 | Degradation of Azo Dyes Decolourization Bacteria and its application |
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