CN114660149A - Electrochemical test paper and detection method - Google Patents
Electrochemical test paper and detection method Download PDFInfo
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- CN114660149A CN114660149A CN202210169426.4A CN202210169426A CN114660149A CN 114660149 A CN114660149 A CN 114660149A CN 202210169426 A CN202210169426 A CN 202210169426A CN 114660149 A CN114660149 A CN 114660149A
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- 238000000840 electrochemical analysis Methods 0.000 title claims abstract description 48
- 238000001514 detection method Methods 0.000 title claims abstract description 39
- 238000006243 chemical reaction Methods 0.000 claims abstract description 89
- 230000035484 reaction time Effects 0.000 claims abstract description 19
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 55
- 229910052709 silver Inorganic materials 0.000 claims description 55
- 239000004332 silver Substances 0.000 claims description 55
- 239000008280 blood Substances 0.000 claims description 42
- 210000004369 blood Anatomy 0.000 claims description 42
- 102000001554 Hemoglobins Human genes 0.000 claims description 27
- 108010054147 Hemoglobins Proteins 0.000 claims description 27
- 239000000758 substrate Substances 0.000 claims description 27
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 25
- 239000008103 glucose Substances 0.000 claims description 25
- 238000012360 testing method Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 6
- 230000002209 hydrophobic effect Effects 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 238000011067 equilibration Methods 0.000 claims description 4
- 238000005259 measurement Methods 0.000 abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 18
- 238000003487 electrochemical reaction Methods 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 208000017667 Chronic Disease Diseases 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005534 hematocrit Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 230000027756 respiratory electron transport chain Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
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- 238000012544 monitoring process Methods 0.000 description 1
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- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
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Abstract
The application discloses an electrochemical test paper and a detection method, which belong to the technical field of biosensors, and the detection method comprises the following steps: inserting the electrochemical test paper into an analyzer, and starting the analyzer; applying a reaction voltage to an electrode system of the electrochemical test paper through an analyzer; adding a sample to be tested into the electrochemical test paper; reducing the reaction voltage to a balance voltage, and recording the balance time when the reaction voltage is at the balance voltage; when the equilibrium time reaches a first threshold value, raising the equilibrium voltage to a reaction voltage, and recording the reaction time at the reaction voltage; and when the reaction time reaches a second threshold value, calculating the current value at the moment, and converting the current value to obtain the detection value of the sample to be detected. The application has high measurement accuracy and can meet the requirements of professional markets.
Description
Technical Field
The application relates to the technical field of biosensors, in particular to electrochemical test paper and a detection method.
Background
With the ever-increasing living standard of people and the irregular daily work and rest caused by the work pressure, the risk of suffering from chronic diseases is increasing, for example, diabetes is a typical chronic disease, and the monitoring of blood sugar can better screen and control the blood sugar change of the diabetics.
At present, the electrochemical test paper with an electrode system is widely used for detecting physiological indexes in blood samples, wherein the basic structure of the electrochemical test paper comprises an insulating substrate, an electrode system, a channel forming layer and an upper cover, the electrode system is positioned on the insulating substrate, the electrode system comprises a reaction electrode and a counter electrode, a reaction reagent covers the reaction electrode, a detection sample reacts with the reaction reagent through a channel forming area on the channel forming layer and generates a detectable signal, and an analyzer connected with the electrochemical test paper obtains a detection value according to the signal.
With respect to the related art in the above, the inventors found that: during measurement, the reaction between the detection sample and the reaction reagent is insufficient, the stability is poor, and the measurement error is large.
Disclosure of Invention
In order to improve the measurement accuracy, the application provides an electrochemical test paper and a detection method.
In a first aspect, the present application provides a method for detecting an electrochemical test paper, which adopts the following technical scheme:
a detection method of an electrochemical test paper comprises the following steps:
inserting the electrochemical test paper into an analyzer, and starting the analyzer;
applying a reaction voltage to an electrode system of the electrochemical test paper through an analyzer;
adding a sample to be detected into the electrochemical test paper;
reducing the reaction voltage to a balance voltage, and recording the balance time when the reaction voltage is at the balance voltage;
when the equilibrium time reaches a first threshold value, raising the equilibrium voltage to a reaction voltage, and recording the reaction time at the reaction voltage;
and when the reaction time reaches a second threshold value, calculating the current value at the moment, and converting the current value to obtain the detection value of the sample to be detected.
By adopting the technical scheme, the sample to be detected is added on the electrochemical test paper, the electrochemical test paper is just dry, the sample to be detected is not fully contacted with the chemical reagent on the electrochemical test paper in the process that the sample to be detected infiltrates into the electrochemical test paper, at the moment, if the reaction voltage is always applied to the electrode system, the sample to be detected and the chemical reagent are always subjected to electrochemical reaction, and the loop current is always in an increasing state, so that the subsequent current value is influenced. Through reducing reaction voltage to balanced voltage, make electrode system's return circuit current reduce, there is not return circuit current even, the sample that awaits measuring is more weak or does not have electrochemical reaction with chemical reagent, and keeps a period at balanced voltage, in this period, makes the sample that awaits measuring can fully mix with chemical reagent, contact, then resumes voltage as reaction voltage again, at this moment, can produce stable return circuit current on the electrode system, and then has improved the measuring precision.
Preferably, the step of reducing the reaction voltage to the equilibrium voltage and recording the equilibrium time at the equilibrium voltage comprises:
judging whether a loop current exists on the electrode system;
if so, the analyzer reduces the reaction voltage to the equilibrium voltage and records the equilibrium time when the reaction voltage is at the equilibrium voltage;
if not, the reaction voltage is kept unchanged.
By adopting the technical scheme, a sample to be detected is added into the electrochemical test paper, the sample to be detected permeates into the electrode system, a loop is formed on the electrode system, reaction voltage is applied to the electrode system, at the moment, loop current is applied to the electrode system, and detection action can be identified by detecting the loop current, so that automatic identification can be realized, and the reaction voltage is reduced to balance voltage.
Preferably, the reaction voltage is 0.3V to 0.5V.
Preferably, the reaction voltage is 0.4V, and the equilibrium voltage is 0V.
By adopting the technical scheme, the reaction current is influenced by too high and too low voltage, the electrochemical reaction is possibly insufficient due to low voltage, and interference can be introduced by high voltage. The reaction voltage is 0.4V, so that the electrode system can have more stable current; the balance voltage is 0V, no current exists on the electrode system, the sample to be detected does not have electrochemical reaction with the chemical reagent, and the sample to be detected can be fully mixed and contacted with the chemical reagent conveniently.
Preferably, the equilibration time is 2s and the reaction time is 3 s.
By adopting the technical scheme, the balance time is 2s, so that the sample to be detected can be fully mixed with the chemical reagent conveniently; the reaction time is 3s, so that the sample to be detected can fully react with the chemical reagent, and stable loop current can be obtained.
Preferably, the electrode system includes a first electrode system and a second electrode system, and when the reaction time reaches a second threshold, the current value at that time is calculated, and the current value is converted to obtain the detection value of the sample to be detected, including:
when the reaction time reaches a second threshold value, calculating a first current value of the first electrode system at the moment and a second current value of the second electrode system;
calculating a hemoglobin value according to the first current value, and calculating an initial blood glucose value according to the second current value;
and compensating the initial blood glucose value by utilizing the hemoglobin value according to a compensation formula to obtain the final blood glucose value.
Preferably, the compensation formula is:
Y1=Y0+9.7•lnX-25.3
in the formula: y1 is the final blood glucose value; y0 is the initial blood glucose value; and X is a hemoglobin value.
By adopting the technical scheme, the hematocrit change in the blood, namely the change of the hemoglobin value can cause the difference of the readings of the blood sugar values tested by the electrochemical test paper. Typically, a high hematocrit will exhibit a negative bias, i.e., a high HCT value, and the read blood glucose or lactate will have a lower value; whereas at low HCT values, a positive deviation occurs, i.e. the calculated analyte concentration is higher. The hemoglobin value is detected in real time, and the blood glucose value is compensated by the hemoglobin value, so that the blood glucose value detection accuracy is further improved.
In a second aspect, the present application provides an electrochemical test paper, which adopts the following technical scheme:
an electrochemical test strip suitable for the detection method comprises:
the hemoglobin detector comprises an insulating substrate, wherein a first electrode system and a second electrode system are arranged on the insulating substrate, the first electrode system is used for detecting a hemoglobin value, and the second electrode system is used for detecting a blood glucose value;
the channel forming layer covers the insulating substrate, a channel forming area is arranged on the channel forming layer and is positioned above the first electrode system and the second electrode system;
and the upper cover layer covers the channel forming layer, and a sample injection port is arranged at the position of the upper cover layer corresponding to the channel forming region.
Preferably, the first electrode system comprises a first silver electrode, a second silver electrode, a first contact and a second contact, the first silver electrode and the second silver electrode are positioned at one end of the insulating substrate, the first contact and the second contact are positioned at the other end of the insulating substrate, the first silver electrode is electrically connected with the first contact, the second silver electrode is electrically connected with the second contact, and a test area is arranged between the first silver electrode and the second silver electrode;
the second electrode system comprises a reaction electrode, a counter electrode, a third contact and a fourth contact, the reaction electrode and the counter electrode are positioned at one end of the insulating substrate, which is provided with the first silver electrode, the third contact and the fourth contact are positioned at one end of the insulating substrate, which is provided with the first contact, the reaction electrode is electrically connected with the third contact, the counter electrode is electrically connected with the fourth contact, and a reaction area is arranged between the reaction electrode and the counter electrode;
the channel forming region is located above the first silver electrode and extends from the first silver electrode to the position of the counter electrode.
Through adopting above-mentioned technical scheme, utilize first electrode system to detect the hemoglobin value, utilize second electrode system to detect the blood sugar level, can detect hemoglobin value and blood sugar level simultaneously, raise the efficiency.
Preferably, a hydrophobic insulating area is arranged between the second silver electrode and the reaction electrode.
By adopting the technical scheme, the hydrophobic insulation region plays a role in isolation, and interference between the first electrode system and the second electrode system is reduced.
In summary, the present application includes at least one of the following beneficial technical effects:
1. by reducing the reaction voltage to the balance voltage, the loop current of the electrode system is reduced, even no loop current exists, the electrochemical reaction of the sample to be measured and the chemical reagent is weaker or no, the balance voltage is kept for a period of time, the sample to be measured and the chemical reagent can be fully mixed, contacted and balanced in the period of time, and then the voltage is restored to the reaction voltage, at the moment, the stable loop current can be generated on the electrode system, and the measurement accuracy is improved;
2. the blood glucose value is compensated by detecting the hemoglobin value in real time and utilizing the hemoglobin value, so that the blood glucose value detection accuracy is further improved;
3. the hemoglobin value is detected through the first electrode system, the blood sugar value is detected through the second electrode system, the hemoglobin value and the blood sugar value can be detected simultaneously, and the efficiency is improved.
Drawings
FIG. 1 is a flow chart of a method of detecting an electrochemical test strip in an embodiment of the present application;
FIG. 2 is a schematic diagram of an electrochemical test strip according to an embodiment of the present disclosure.
Description of reference numerals:
1. an insulating substrate; 10. a first silver electrode; 11. a second silver electrode; 12. a first contact; 13. a second contact; 14. a test zone; 15. a reaction electrode; 16. a counter electrode; 17. a third contact; 18. a fourth contact; 19. a reaction zone; 2. a hydrophobic insulating region; 3. a channel forming layer; 30. a channel formation region; 4. an upper cover layer; 40. and (4) injecting a sample port.
Detailed Description
The present application is described in further detail below with reference to figures 1-2.
The embodiment of the application discloses a detection method of electrochemical test paper. Referring to fig. 1, the detection method includes the steps of:
s100: the electrochemical test strip is inserted onto the analyzer and the analyzer is started.
Specifically, the electrochemical test strip is inserted into an analyzer at the end with the contact, and then the analyzer is started, and the analyzer can be a blood glucose meter.
S200: a reaction voltage is applied to the electrode system of the electrochemical test strip by the analyzer.
Specifically, the electrochemical test paper is inserted on an analyzer, a power supply end and a grounding end of the analyzer are respectively connected with two electrodes of the electrode system, and at the moment, the electrode system is in an open circuit state. The reaction voltage is set on the analyzer and may be set to 0.3V to 0.5V, and in this embodiment, the reaction voltage may be set to 0.4V.
S300: adding a sample to be detected into the electrochemical test paper.
Specifically, the sample injection port 40 of the electrochemical test paper is dropped with a detection sample, and the detection sample can cover the electrode system.
S400: the reaction voltage is reduced to the equilibrium voltage and the equilibrium time at the equilibrium voltage is recorded.
Specifically, when the analyzer detects that the detection sample has been dropped into, the analyzer drops the reaction voltage, and the voltage on the electrode system is dropped to the equilibrium voltage, in this embodiment, the equilibrium voltage can be set to 0V, and the analyzer drops the voltage of the electrode system from 0.4V to 0V, and at this moment, there is no loop current on the electrode system, and the time when the voltage of the electrode system is at 0V is the equilibrium time, and starts to time.
Meanwhile, in the time period, the detection sample permeates and fuses on the electrochemical test paper, and the sample to be detected can be fully mixed and contacted with the chemical reagent on the electrode system.
S500: when the equilibration time reaches a first threshold, the equilibration voltage is raised to a reaction voltage and the reaction time at the reaction voltage is recorded.
Specifically, the first threshold may be set to 2s, when the time when the voltage of the electrode system is at 0V reaches 2s, the analyzer increases the voltage of the electrode system from 0V to 0.4V, the analyzer increases the voltage of the electrode system to 0.4V, and the time maintained at 0.4V is the reaction time, and the analyzer starts to count time.
Meanwhile, the sample to be detected can be in contact with the electrode system, so that the electrode system forms a power-on loop, the voltage of the electrode system is 0.4V, loop current is generated on the electrode system, and the sample to be detected and the chemical reagent on the electrode system perform electrochemical reaction.
S600: and when the reaction time reaches a second threshold value, calculating the current value at the moment, and converting the current value to obtain the detection value of the sample to be detected.
Specifically, the second threshold may be set to 3s, and when the reaction time reaches 3s, the analyzer measures a current value of the loop current on the electrode system at that time, converts the current value to obtain a detection value, and displays the detection value. After the detection is completed, the voltage on the electrode system becomes 0V.
Optionally, in step S400, reducing the response voltage to the equilibrium voltage, and recording the equilibrium time at the equilibrium voltage, includes the following sub-steps:
s401: and judging whether the electrode system has loop current or not.
S402: if yes, the analyzer reduces the reaction voltage to the equilibrium voltage, and records the equilibrium time when the reaction voltage is at the equilibrium voltage.
S403: if not, the reaction voltage is kept unchanged.
Specifically, the electrode system comprises a first electrode system and a second electrode system, the electrochemical test paper is inserted into the analyzer, and the analyzer applies 0.4V voltage to the first electrode system and the second electrode system. Dripping samples to be tested on the first electrode system and the second electrode system, wherein the samples to be tested cover the test area 14 of the first electrode system, so that the first electrode system forms an electrifying loop; the sample to be measured covers the reaction area 19 of the second electrode system, so that the second electrode system forms an electrifying loop, when the analyzer detects that loop current exists at any position of the first electrode system and the second electrode system, the detection can be indicated to be started, and the analyzer changes the voltage on the first electrode system and the second electrode system into 0V.
Optionally, in step S600, that is, when the reaction time reaches the second threshold, calculating a current value at that time, and converting the current value to obtain a detection value of the sample to be detected, the method includes the following substeps:
s601: when the reaction time reaches a second threshold value, a first current value of the first electrode system at the moment and a second current value of the second electrode system are calculated.
S602: a hemoglobin value is calculated from the first current value, and an initial blood glucose value is calculated from the second current value.
S603: and compensating the initial blood glucose value by utilizing the hemoglobin value according to a compensation formula to obtain the final blood glucose value.
Specifically, the analyzer detects a current between the first silver electrode 10 and the second silver electrode 11 to obtain a first current value, and converts the first current value into a hemoglobin value; the analyzer detects the current between the reaction electrode 15 and the counter electrode 16 to obtain a second current value, and converts the second current value to obtain an initial blood sugar value, and calculates the initial blood sugar value by using a compensation formula to obtain a final blood sugar value. Wherein, the compensation formula is:
Y1=Y0+9.7•lnX-25.3
in the formula: y1 is the final blood glucose value; y0 is the initial blood glucose value; and X is a hemoglobin value.
The implementation principle of the detection method of the electrochemical test paper in the embodiment of the application is as follows: through dropping reaction voltage to balanced voltage, make electrode system's return circuit current reduce, there is not return circuit current even, the sample that awaits measuring is more weak or do not have electrochemical reaction with chemical reagent's electrochemical reaction, and keep a period at balanced voltage, in this period, make the sample that awaits measuring can fully mix with chemical reagent, the contact, then resume the voltage to reaction voltage, at this moment, can produce stable return circuit current on the electrode system, and compensate blood sugar value according to hemoglobin value, blood sugar value measuring precision can effectively have improved.
Referring to fig. 2, the embodiment of the present application further discloses an electrochemical test paper, which is suitable for the detection method, the electrochemical test paper includes an insulating substrate 1, a channel forming layer 3, and an upper cover layer 4 in sequence, and the insulating substrate 1, the channel forming layer 3, and the upper cover layer 4 are all made of insulating materials.
A first electrode system for detecting a hemoglobin value and a second electrode system for detecting a blood glucose value are provided on the surface of the insulating substrate 1 facing the channel formation layer 3.
The first electrode system comprises a first silver electrode 10, a second silver electrode 11, a first contact 12 and a second contact 13, wherein the first silver electrode 10 and the second silver electrode 11 are positioned at one end of the insulating substrate 1, a test area 14 is arranged between the first silver electrode 10 and the second silver electrode 11, and the test area 14 does not contain any chemical reagent. The first contact 12 and the second contact 13 are located at the other end of the insulating substrate 1, the first silver electrode 10 is electrically connected to the first contact 12, and the second silver electrode 11 is electrically connected to the second contact 13.
The test area 14 is arranged, so that an open circuit is formed between the first silver electrode 10 and the second silver electrode 11; the sample to be tested is covered on the test area 14, 0.4V voltage is applied between the first silver electrode 10 and the second silver electrode 11, and the hemoglobin value and the silver electrode can directly carry out electron transfer, so that a first current is formed between the first silver electrode 10 and the second silver electrode 11.
The second electrode system comprises a reaction electrode 15, a counter electrode 16, a third contact 17 and a fourth contact 18, wherein the reaction electrode 15 and the counter electrode 16 are positioned at one end of the insulating substrate 1 with the first silver electrode 10, the counter electrode 16 is positioned at one side of the reaction electrode 15 far away from the second silver electrode 11, and a hydrophobic insulating area 2 is arranged between the second silver electrode 11 and the reaction electrode 15. A reaction zone 19 is arranged between the reaction electrode 15 and the counter electrode 16, and an enzyme layer is covered in the reaction zone 19. The third contact 17 and the fourth contact 18 are located at the end of the insulating substrate 1 having the first contact 12, the reaction electrode 15 is electrically connected to the third contact 17, and the counter electrode 16 is electrically connected to the fourth contact 18.
By providing the reaction zone 19, an open circuit is formed between the reaction electrode 15 and the counter electrode 16; the sample to be tested is covered on the reaction area 19, 0.4V voltage is applied between the reaction electrode 15 and the counter electrode 16, the blood sugar and the enzyme layer generate electrochemical reaction to form electrons and generate transfer, and a second current is formed between the reaction electrode 15 and the counter electrode 16.
The length of the channel forming layer 3 is smaller than that of the insulating substrate 1, and the first contact 12, the second contact 13, the third contact 17, and the fourth contact 18 are at least partially exposed to the air when the channel forming layer 3 is covered on the insulating substrate 1. The channel forming layer 3 is provided with a channel forming region 30 corresponding to the first silver electrode 10, and the channel forming region 30 extends from the first silver electrode 10 to the counter electrode 16.
The length of the upper cover layer 4 is the same as that of the channel forming layer 3, the upper cover layer 4 covers the surface of the channel forming layer 3 departing from the insulating substrate 1, and a sample injection port 40 is formed in the position, corresponding to the channel forming region 30, of the upper cover layer 4.
The first contact 12, the second contact 13, the third contact 17 and the fourth contact 18 are exposed to the air, and when the electrochemical test paper is inserted into the analyzer, the analyzer can apply a voltage of 0.4V between the first silver electrode 10 and the second silver electrode 11, and between the reaction electrode 15 and the counter electrode 16. By providing the channel-forming region 30 and the sample injection port 40, the sample to be tested can cover the test region 14 and the reaction region 19 when the sample to be tested is dropped on the electrochemical test paper.
The implementation principle of the electrochemical test paper in the embodiment of the application is as follows: the sample to be tested covers the test area 14, 0.4V voltage is applied between the first silver electrode 10 and the second silver electrode 11, and the hemoglobin value and the silver electrode can be directly subjected to electron transfer, so that a first current is formed between the first silver electrode 10 and the second silver electrode 11, the first current is detected, and the hemoglobin value can be obtained through conversion. The sample to be detected is covered on the reaction area 19, 0.4V voltage is applied between the reaction electrode 15 and the counter electrode 16, the blood sugar and the enzyme layer generate electrochemical reaction to form electrons and generate transmission, so that a second current is formed between the reaction electrode 15 and the counter electrode 16, the second current is detected, and the initial blood sugar value can be obtained through conversion.
The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (10)
1. The detection method of the electrochemical test paper is characterized by comprising the following steps:
inserting the electrochemical test paper into an analyzer, and starting the analyzer;
applying a reaction voltage to an electrode system of the electrochemical test paper through an analyzer;
adding a sample to be detected into the electrochemical test paper;
reducing the reaction voltage to a balance voltage, and recording the balance time when the reaction voltage is at the balance voltage;
when the equilibrium time reaches a first threshold value, raising the equilibrium voltage to a reaction voltage, and recording the reaction time at the reaction voltage;
and when the reaction time reaches a second threshold value, calculating the current value at the moment, and converting the current value to obtain the detection value of the sample to be detected.
2. The method of claim 1, wherein the step of reducing the response voltage to an equilibrium voltage and recording an equilibrium time at the equilibrium voltage comprises:
judging whether a loop current exists on the electrode system;
if so, the analyzer reduces the reaction voltage to the equilibrium voltage and records the equilibrium time when the reaction voltage is at the equilibrium voltage;
if not, the reaction voltage is kept unchanged.
3. The detection method according to claim 2, wherein the reaction voltage is 0.3V to 0.5V.
4. The detection method according to claim 3, wherein the reaction voltage is 0.4V and the equilibrium voltage is 0V.
5. The method of claim 2, wherein the equilibration time is 2s and the reaction time is 3 s.
6. The detection method according to claim 1, wherein the electrode system comprises a first electrode system and a second electrode system, and when the reaction time reaches a second threshold value, calculating a current value at that time, and converting the current value to obtain a detection value of the sample to be detected comprises:
when the reaction time reaches a second threshold value, calculating a first current value of the first electrode system at the moment and a second current value of the second electrode system;
calculating a hemoglobin value according to the first current value, and calculating an initial blood glucose value according to the second current value;
and compensating the initial blood glucose value by utilizing the hemoglobin value according to a compensation formula to obtain the final blood glucose value.
7. The detection method according to claim 6, wherein the compensation formula is:
Y1=Y0+9.7•lnX-25.3
in the formula: y1 is the final blood glucose value; y0 is the initial blood glucose value; and X is a hemoglobin value.
8. An electrochemical test strip suitable for use in the detection method of any one of claims 1 to 7, comprising:
the blood glucose monitor comprises an insulating substrate (1), wherein a first electrode system and a second electrode system are arranged on the insulating substrate (1), the first electrode system is used for detecting a hemoglobin value, and the second electrode system is used for detecting a blood glucose value;
the channel forming layer (3) covers the insulating substrate (1), a channel forming area (30) is arranged on the channel forming layer (3), and the channel forming area (30) is located above the first electrode system and the second electrode system;
the upper cover layer (4) covers the channel forming layer (3), and a sample injection port (40) is formed in the position, corresponding to the channel forming area (30), of the upper cover layer (4).
9. The electrochemical test paper according to claim 8, wherein the first electrode system comprises a first silver electrode (10), a second silver electrode (11), a first contact (12) and a second contact (13), the first silver electrode (10) and the second silver electrode (11) are positioned at one end of the insulating substrate (1), the first contact (12) and the second contact (13) are positioned at the other end of the insulating substrate (1), the first silver electrode (10) is electrically connected with the first contact (12), the second silver electrode (11) is electrically connected with the second contact (13), and a test area (14) is arranged between the first silver electrode (10) and the second silver electrode (11);
the second electrode system comprises a reaction electrode (15), a counter electrode (16), a third contact (17) and a fourth contact (18), the reaction electrode (15) and the counter electrode (16) are positioned at one end of the insulating substrate (1) with the first silver electrode (10), the third contact (17) and the fourth contact (18) are positioned at one end of the insulating substrate (1) with the first contact (12), the reaction electrode (15) is electrically connected with the third contact (17), the counter electrode (16) is electrically connected with the fourth contact (18), and a reaction area (19) is arranged between the reaction electrode (15) and the counter electrode (16);
the channel forming region (30) is located above the first silver electrode (10) and extends from the first silver electrode (10) to the position of the counter electrode (16).
10. The electrochemical test strip according to claim 9, characterized in that a hydrophobic insulating region (2) is provided between the second silver electrode (11) and the reaction electrode (15).
Priority Applications (1)
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