CN114656032A - Method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic particles - Google Patents

Method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic particles Download PDF

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CN114656032A
CN114656032A CN202210436861.9A CN202210436861A CN114656032A CN 114656032 A CN114656032 A CN 114656032A CN 202210436861 A CN202210436861 A CN 202210436861A CN 114656032 A CN114656032 A CN 114656032A
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sludge
salt
granulation
aerobic
tolerant
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欧栋
艾宁
任杰
严泉旺
赖常义
胡长伟
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Jiaxing University
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • C02F3/12Activated sludge processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • C02F3/12Activated sludge processes
    • C02F3/1236Particular type of activated sludge installations
    • C02F3/1263Sequencing batch reactors [SBR]
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

Abstract

The invention provides a method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic granules, which comprises the following steps: 1) salt-tolerant aerobic granular sludge is sequentially put into a Sequencing Batch Reactor (SBR), and the salt-tolerant aerobic granular sludge is formed by mixing aerobic activated sludge and anaerobic granular sludge according to the volume ratio of 1: 1; 2) artificially synthesized simulated domestic sewage is used as inlet water, and the inoculated sludge is subjected to granulation culture in an SBR reactor; 3) operation regulation and control: during the operation of the whole SBR reactor, the salinity level is increased according to the gradient of increasing 1 percent every 14 days, the sedimentation condition of the sludge is observed, the sedimentation time of the sludge is continuously shortened, and the sedimentation time is stopped to be adjusted when the granular sludge is primarily formed; 4) regularly microscopic examination is carried out on the sludge, and the particle size distribution condition of the granular sludge in the SBR reactor is detected: when the proportion of the granular sludge larger than 0.2 mm reaches more than 70 percent, the start of the aerobic granular sludge system is considered to be finished. The method is simple and strong in operability, and effectively accelerates the formation process of the salt-tolerant granular sludge.

Description

Method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic particles
Technical Field
The invention belongs to the technical field of sewage biotechnological treatment, and particularly relates to a method for strengthening salt-tolerant aerobic sludge granulation by adding anaerobic granules.
Background
The traditional activated sludge process has proven to be a viable and economically efficient treatment process for salt-containing wastewater that can meet the worldwide wastewater treatment demand of 5%. Aerobic granular sludge is an upgrading process based on the activated sludge process, and is receiving increasing attention as a promising biological treatment process due to its excellent settling properties, higher sludge concentration and lower reactor volume. Under the large background of 'carbon peak reaching' and 'carbon neutralization', a new efficient and energy-saving sewage treatment process needs to be explored urgently.
Research reports that factors such as substrate composition, organic load, shearing force, settling time, hydraulic retention time, reactor configuration and the like can cause great influence on the formation and long-term stable operation of aerobic granular sludge. Meanwhile, different types of inoculation flora and sludge are selected to achieve the purposes of shortening the starting time of the aerobic granular sludge and strengthening the performance of the aerobic granular sludge. For example, the inoculation of the aerobic granular sludge stored at-20 ℃ can effectively shorten the time required by sludge granulation, and the cultured mature aerobic granular sludge has good settling property and degradation property. Liu et al developed an aerobic granulation method using a self-polymerizing strain Rhizobium sp. NJUST18, which promotes the formation of aerobic granular sludge by utilizing the aggregation property of the strain itself. Liang and the like accelerate the granulation process by using pyrimidine degrading bacteria (NJUST 18+ NJUST 29), and the formed aerobic granular sludge has stronger agglomeration capacity and pyrimidine degrading capacity. Thus, the type of inoculated sludge plays a key role in the formation and performance of aerobic granules.
Therefore, the invention provides a method for using anaerobic granular sludge and a small amount of activated sludge as inoculated sludge, which achieves the purposes of accelerating the formation process of salt-resistant granular sludge and strengthening the salt resistance of aerobic granular sludge.
Disclosure of Invention
The invention aims to solve the technical problems of long culture time and poor salt tolerance of aerobic granular sludge in the prior art and provides a method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic granules. According to the method, anaerobic granular sludge is added, a small amount of activated sludge is combined to serve as a method for inoculating sludge, the salt concentration is gradually improved, the operation parameters of the reactor such as sludge settling time are gradually shortened, and the like are optimized, so that the purposes of accelerating the formation process of salt-resistant granular sludge and strengthening the salt resistance of aerobic granular sludge are achieved.
The purpose of the invention and the technical problem to be solved are realized by adopting the following technical scheme.
One aspect of the invention provides a method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic granules, which comprises the following steps:
1) salt-tolerant aerobic granular sludge is sequentially put into a Sequencing Batch Reactor (SBR), and the salt-tolerant aerobic granular sludge is formed by mixing aerobic activated sludge and anaerobic granular sludge according to the volume ratio of 1: 1;
2) artificially synthesized simulated domestic sewage is used as inlet water, and the seeded sludge is subjected to granulation culture in an SBR reactor;
3) operation regulation and control: during the operation of the whole SBR reactor, the salinity level is increased according to the gradient of increasing 1 percent every 14 days, the settlement condition of the sludge is observed, the settlement time of the sludge is continuously shortened, and the settlement time is stopped being adjusted when the granular sludge is preliminarily formed;
4) regularly microscopic examination is carried out on the sludge, and the particle size distribution condition of the granular sludge in the SBR reactor is detected: when the proportion of the granular sludge larger than 0.2 mm reaches more than 70 percent, the start of the aerobic granular sludge system is considered to be finished.
Preferably, the suspended solid of the initial mixed liquid in the SBR reactor in the step 1) is 5.3 +/-0.1 g/L, and the sludge volume index is 147.7 +/-0.2 mL/g.
Preferably, the simulated wastewater in the step 2) is composed of: NH (NH)4Cl 500~650 mg/L;KH2PO4 140~200 mg/L;CaCl 2 150~200 mg/L;MgCl2 31~86 mg/L;FeSO4·7H2O 10~20 mg/L。
Preferably, trace elements can be further added into the simulated wastewater, and the volume ratio of the trace elements to the simulated wastewater is 0.05 mL: 1L of the total amount of the active ingredients.
Preferably, the trace elements consist of: h3BO3 50~60 mg/L;ZnCl2 50 ~60 mg/L;CuCl2 30~40 mg/L;MnSO4·H2O 50~65 mg/L;(NH4)6Mo7O24·4H2O 50~65 mg/L;AlCl3 50~65 mg/L;CoCl2·6H2O 50~65 mg/L;NiCl2 50~65 mg/L。
Preferably, the SBR reactor described in step 3) is operated for 4 cycles per day, wherein each operation cycle is operated in a water-in-anaerobic-aeration-sedimentation-water-out manner, and each operation cycle is 6 hours.
Preferably, in one operation period of the SBR reactor in the step 3), the water inlet time is 5 minutes, the anaerobic time is 0-30 minutes, the aeration time is 315-320 minutes, the sedimentation time is 3-30 minutes, and the water outlet time is 5 minutes.
Preferably, the COD of the inlet water in the step 3) is maintained at 1000-2500 mg/L.
Preferably, step 3) is performed by adding 2M NaHCO3Or HCl solution to maintain the feed water pH at 7.0 + -0.1.
Preferably, the salinity level is increased in step 3) by NaCl.
The invention has the following beneficial effects: in the process of culturing the salt-tolerant aerobic granular sludge, the salt concentration is gradually improved by artificially feeding the anaerobic granular sludge and combining a small amount of activated sludge as inoculated sludge, the salt-tolerant aerobic granular sludge is induced to be generated, the purposes of accelerating the formation process of the salt-tolerant granular sludge and strengthening the salt resistance of the aerobic granular sludge are achieved by optimizing reactor operation parameters such as gradually shortening the sludge settling time and the like, and the method for strengthening the granulation of the salt-tolerant aerobic sludge is provided for the application of the aerobic granular sludge technology to the treatment of high-salt industrial wastewater.
Drawings
FIG. 1 shows a schematic representation of the operation of the SBR reactor according to the invention.
Detailed Description
The invention is further described below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "left", "right", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation and a specific orientation configuration and operation, and thus, should not be construed as limiting the present invention. Furthermore, "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature.
In the description of the present invention, "a plurality" means two or more unless otherwise specified. In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate member, or they may be connected through two or more elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in FIG. 1, one embodiment of the present invention employs a laboratory-scale SBR reactor to culture salt-tolerant aerobic granular sludge and operate stably for about 160 days. The inoculated sludge consists of aerobic activated sludge and anaerobic granular sludge, and the volume ratio of the aerobic activated sludge to the anaerobic granular sludge is 1:1, and the total volume is 100 mL. The whole reactor system is controlled by a PLC, the reactor runs continuously and runs for 4 periods every day, and each period is 6. The Volume Exchange Ratio (VER) of the reactor operating cycle was 50%. Regulating and controlling the temperature within the range of 22 +/-2 ℃. Each operation period comprises 5 min water inlet, 0-30 min anaerobic phase, 315-320 min aeration phase, 3-30 min sedimentation phase and 5 min water outlet phase. In order to optimize the reactor performance better, the reactor operating parameters, such as settling time, influent organic loading and aerobic/anaerobic time, were adjusted and optimized during 160 d operation, and the parameters of the entire operation are shown in table 1.
TABLE 1 operating conditions and Performance of aerobic granular sludge at different salinity
Figure 761013DEST_PATH_IMAGE001
The aerobic activated sludge used in the experiment is taken from the return sludge of a secondary sedimentation tank of a long-bridge municipal sewage treatment plant in Shanghai city, and the anaerobic granular sludge is derived from an anaerobic bioreactor built in the laboratory. The inoculated sludge consists of aerobic activated sludge and anaerobic granular sludge, and is mixed according to the volume ratio of 1:1, and the total volume is 100 mL. The initial Mixed Liquor Suspended Solids (MLSS) in the SBR system is 5.3 +/-0.1 g/L, and preferably 5.3 g/L in the embodiment; the Sludge Volume Index (SVI) was 147.7 + -0.2 mL/g, in this example 147.7 mL/g. Glucose is added into the inlet water as a carbon source, the COD of the inlet water is maintained at 1000-2500 mg/L, and the operation condition and the removal efficiency of the COD are shown in Table 1. The simulated wastewater comprises the following components: NH (NH)4Cl 500~650 mg/L;KH2PO4 140~200 mg/L;CaCl 2 150~200 mg/L;MgCl2 31~86 mg/L;FeSO4·7H2O10-20 mg/L, preferably (mg/L): NH (NH)4Cl (500);KH2PO4(140);CaCl2(150); MgCl2(31) (ii) a And FeSO4·7H2And O (10). 2M NaHCO is added3Or the HCl solution makes the pH value of the simulated wastewater in the range of 7.0 +/-0.1 when the wastewater enters the water. Trace elements can also be added into the simulated wastewater, and the trace elements comprise: h3BO3 50~60 mg/L;ZnCl2 50 ~60 mg/L;CuCl2 30~40 mg/L;MnSO4·H2O 50~65 mg/L;(NH4)6Mo7O24·4H2O 50~65 mg/L;AlCl3 50~65 mg/L;CoCl2·6H2O 50~65 mg/L;NiCl250-65 mg/L. In this embodiment, 2.5 mL of the trace element solution is preferably added to 50L of the simulated wastewater, and the specific composition is (mg/L): h3BO3(50); ZnCl2 (50); CuCl2(30); MnSO4·H2O (50); (NH4)6Mo7O24·4H2O (50); AlCl3(50);CoCl2·6H2O (50); NiCl2(50)。
In the SBR system, conventional aerobic activated sludge and anaerobic granular sludge are used as inoculation sludge, the oriented cultivation of salt-tolerant aerobic granular sludge is carried out in three different stages (figure 1), and the operation condition of the SBR system is shown in figure 1. And analyzing indexes such as MLSS, MLVSS, SVI and COD removal efficiency and the like in each operation stage.
Figure 146995DEST_PATH_IMAGE002
And in the starting stage, anaerobic granular sludge is added into the SBR reactor as inoculation sludge, aerobic activated sludge is gradually adapted to salinity stress under the condition of lower salinity, the operation condition of the reactor is optimized, and water inlet parameters are adjusted. The addition of the anaerobic granular sludge accelerates the granulation process of the salt-tolerant aerobic granular sludge, and simultaneously, the addition of the anaerobic granules strengthens the structural stability of a salt-tolerant aerobic granular sludge system;
Figure 5361DEST_PATH_IMAGE003
a domestication stage under salt pressure, namely, the salt concentration is increased in a stepped manner, the settling time is shortened, young salt-tolerant aerobic granular sludge is gradually formed in the SBR reaction, and non-salt-tolerant flora in an aerobic granular sludge system is gradually eliminated under salt stress;
Figure 869412DEST_PATH_IMAGE004
and a salinity raising stage, namely, the mature salt-tolerant aerobic granular sludge is gradually formed under the condition of high salinity, and the salt-tolerant aerobic granular sludge occupies a dominant position in the SBR reactor.
Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (10)

1. A method for strengthening salt-tolerant aerobic sludge granulation by adding anaerobic granules is characterized by comprising the following steps:
1) salt-tolerant aerobic granular sludge is sequentially put into a Sequencing Batch Reactor (SBR), and the salt-tolerant aerobic granular sludge is formed by mixing aerobic activated sludge and anaerobic granular sludge according to the volume ratio of 1: 1;
2) artificially synthesized simulated domestic sewage is used as inlet water, and the seeded sludge is subjected to granulation culture in an SBR reactor;
3) operation regulation and control: during the operation of the whole SBR reactor, the salinity level is increased according to the gradient of increasing 1 percent every 14 days, the settlement condition of the sludge is observed, the settlement time of the sludge is continuously shortened, and the settlement time is stopped being adjusted when the granular sludge is preliminarily formed;
4) regularly microscopic examination is carried out on the sludge, and the particle size distribution condition of the granular sludge in the SBR reactor is detected: when the proportion of the granular sludge larger than 0.2 mm reaches more than 70 percent, the start of the aerobic granular sludge system is considered to be finished.
2. The method for intensifying salt-tolerant aerobic sludge granulation by adding anaerobic granules according to claim 1, wherein the suspended solids of the initial mixed liquor in the SBR reactor in the step 1) are 5.3 +/-0.1 g/L, and the sludge volume index is 147.7 +/-0.2 mL/g.
3. The method for strengthening salt-tolerant aerobic sludge granulation by adding anaerobic granules according to claim 1, wherein the simulated wastewater in the step 2) comprises the following components: NH (NH)4Cl 500~650 mg/L;KH2PO4 140~200 mg/L;CaCl2150~200 mg/L;MgCl2 31~86 mg/L;FeSO4·7H2O 10~20 mg/L。
4. The method for strengthening salt-tolerant aerobic sludge granulation by adding the anaerobic granules into the simulated wastewater according to the claim 1 or 3, wherein trace elements can be further added into the simulated wastewater, and the volume ratio of the trace elements to the simulated wastewater is 0.05 mL: 1L of the compound.
5. The method for enhancing granulation of salt-tolerant aerobic sludge by adding anaerobic granules according to claim 4, wherein the trace elements comprise: h3BO3 50~60 mg/L;ZnCl2 50 ~60 mg/L;CuCl2 30~40 mg/L;MnSO4·H2O 50~65 mg/L;(NH4)6Mo7O24·4H2O 50~65 mg/L;AlCl3 50~65 mg/L;CoCl2·6H2O 50~65 mg/L;NiCl2 50~65 mg/L。
6. The method for intensifying salt-tolerant aerobic sludge granulation by adding anaerobic granules according to claim 1, wherein the SBR reactor in the step 3) is operated for 4 periods every day, wherein each operation period is operated in a mode of water inlet-anaerobic-aeration-sedimentation-water outlet, and each operation period is 6 hours.
7. The method for strengthening salt-tolerant aerobic sludge granulation by adding anaerobic granules as claimed in claim 6, wherein in the operation period of the SBR reactor in step 3), the water inlet time is 5 minutes, the anaerobic time is 0-30 minutes, the aeration time is 315-320 minutes, the sedimentation time is 3-30 minutes, and the water outlet time is 5 minutes.
8. The method for strengthening salt-tolerant aerobic sludge granulation by adding anaerobic granules according to claim 1, wherein the COD of the influent water in step 3) is maintained at 1000-2500 mg/L.
9. The method for strengthening salt-tolerant aerobic sludge granulation by adding anaerobic granules according to claim 1, wherein in the step 3), 2M NaHCO is added3Or HCl solution to maintain the feed water pH at 7.0 + -0.1.
10. The method for intensifying granulation of salt-tolerant aerobic sludge by adding anaerobic granules according to claim 1, wherein the salinity level is increased by NaCl in the step 3).
CN202210436861.9A 2022-04-25 2022-04-25 Method for strengthening granulation of salt-tolerant aerobic sludge by adding anaerobic particles Pending CN114656032A (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62279892A (en) * 1986-05-30 1987-12-04 Kobe Steel Ltd Formation of sludge particle in anaerobic biological bed
CN101054232A (en) * 2007-05-31 2007-10-17 北京师范大学 Highly efficient treatment process for waster water with high content of salt
CN106145327A (en) * 2016-08-02 2016-11-23 浙江工业大学 A kind of promote aerobic particle mud to be formed and stable compound feed intake and preparation method thereof
CN106219743A (en) * 2016-08-31 2016-12-14 中国石油集团川庆钻探工程有限公司 A kind of method for culturing aerobic granular sludge processed for fracturing outlet liquid
CN106242045A (en) * 2016-09-22 2016-12-21 湖南大学 A kind of aerobic particle mud fast culture process
CN106348432A (en) * 2016-11-02 2017-01-25 西华大学 Rapid culturing method for aerobic granular sludge for treating printing and dying waste water
CN107364956A (en) * 2017-08-07 2017-11-21 北京化工大学 The method and batch-type reaction unit of a kind of rapidly cultivating aerobic particle sludge
CN109607767A (en) * 2018-12-19 2019-04-12 山东大学 A method of culture aerobic particle mud is accelerated based on NaCl concentration incremental manner

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62279892A (en) * 1986-05-30 1987-12-04 Kobe Steel Ltd Formation of sludge particle in anaerobic biological bed
CN101054232A (en) * 2007-05-31 2007-10-17 北京师范大学 Highly efficient treatment process for waster water with high content of salt
CN106145327A (en) * 2016-08-02 2016-11-23 浙江工业大学 A kind of promote aerobic particle mud to be formed and stable compound feed intake and preparation method thereof
CN106219743A (en) * 2016-08-31 2016-12-14 中国石油集团川庆钻探工程有限公司 A kind of method for culturing aerobic granular sludge processed for fracturing outlet liquid
CN106242045A (en) * 2016-09-22 2016-12-21 湖南大学 A kind of aerobic particle mud fast culture process
CN106348432A (en) * 2016-11-02 2017-01-25 西华大学 Rapid culturing method for aerobic granular sludge for treating printing and dying waste water
CN107364956A (en) * 2017-08-07 2017-11-21 北京化工大学 The method and batch-type reaction unit of a kind of rapidly cultivating aerobic particle sludge
CN109607767A (en) * 2018-12-19 2019-04-12 山东大学 A method of culture aerobic particle mud is accelerated based on NaCl concentration incremental manner

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Application publication date: 20220624