CN114651985A - Probiotic powder with weight-losing effect and preparation method thereof - Google Patents

Probiotic powder with weight-losing effect and preparation method thereof Download PDF

Info

Publication number
CN114651985A
CN114651985A CN202210379721.2A CN202210379721A CN114651985A CN 114651985 A CN114651985 A CN 114651985A CN 202210379721 A CN202210379721 A CN 202210379721A CN 114651985 A CN114651985 A CN 114651985A
Authority
CN
China
Prior art keywords
weight
powder
probiotic powder
parts
stirring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210379721.2A
Other languages
Chinese (zh)
Inventor
王冠
张法权
田平平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Mujia Health Industry Co ltd
Original Assignee
Guangzhou Mujia Health Industry Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Mujia Health Industry Co ltd filed Critical Guangzhou Mujia Health Industry Co ltd
Priority to CN202210379721.2A priority Critical patent/CN114651985A/en
Publication of CN114651985A publication Critical patent/CN114651985A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3526—Organic compounds containing nitrogen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention belongs to the technical field of microbial preparations, and particularly relates to probiotic powder with a weight-losing effect and a preparation method thereof. The probiotic powder with the weight-losing effect is prepared from composite probiotics, prebiotics, fig juice, an ophiopogon root extract, water-soluble dietary fibers, an additive, propolis and protamine. The probiotic powder with the weight-losing effect has a good weight-losing effect, does not rebound after weight losing, and has a certain regulation effect on intestinal flora. In addition, the probiotic powder with the weight-losing effect is in a dormant state before being eaten, and still keeps a large amount of activity when being rehydrated.

Description

Probiotic powder with weight-losing effect and preparation method thereof
Technical Field
The invention belongs to the technical field of microbial preparations, and particularly relates to probiotic powder with a weight-losing effect and a preparation method thereof.
Background
In recent years, due to the rise of socioeconomic level and the improvement of life quality of people, many overweight obese people exist in people around us. The obese people not only influence the self image, but also bring much invariance to life and work, and also greatly influence the personal health. The causes of obesity include the following intrinsic factors, in addition to hyperphagia, lack of exercise, genetic problems, and the like: intestinal flora, chronic inflammation and hormones. Not only are the energy metabolism imbalanced in obese people, but the composition of the intestinal flora also differs from that of normal weight.
Probiotics are active microorganisms which are beneficial to a host and change the composition of flora at a certain part of the host by colonizing in a human body. The probiotics can improve the glucose and fat metabolism level, and can also regulate the problem of liver dysfunction caused by high-fat eating, thereby improving insulin sensitivity and steatosis symptoms and helping weight loss. Meanwhile, after the probiotics enter the intestinal tract, the symptoms of host chronic inflammation can be relieved, unbalanced intestinal flora is relatively normalized, the permeability of intestinal epithelial cells is reduced, inflammatory factors are reduced, and a series of metabolic syndrome caused by obesity can be effectively prevented and treated. In addition, the probiotics can effectively increase gastrointestinal hormone, reduce secretion level of ghrelin, effectively inhibit gastrointestinal motility, increase satiety, and reduce weight and fat accumulation. Therefore, probiotic weight loss is one of the most prevalent weight loss modalities today. The probiotics can control the weight to achieve the purpose of losing weight by influencing the appetite and metabolism of people, the burden on the body is small, but the acid and choline resistance of the probiotics is weak, and the actual absorption and weight-losing effect of the probiotics are influenced.
The common probiotics preparation comprises a liquid preparation and a powder preparation, wherein the liquid preparation is easy to be polluted or the strains are degraded along with the prolonging of the preservation time, the liquid preparation needs to activate nutrition and expand culture before use, and the powder preparation usually needs to be dried by adopting vacuum freeze drying, spray drying, fluidized bed drying, microwave vacuum drying and the like. By adopting vacuum freeze drying, the thallus subcellular injury is less, the viable bacteria rate is high, but the thallus subcellular injury is easily influenced by factors such as pre-freezing temperature, freezing speed, vacuum degree and the like, the time consumption is long, and the cost is high; fluidized bed drying is adopted, the drying time is long, and when the material residence time is not uniform, the drying is not uniform; microwave vacuum drying is adopted, polar molecules (such as thallus moisture) synchronously rotate at high speed along with microwave frequency, the materials instantaneously generate friction heat, the surface and the interior of the materials are simultaneously heated, and a large number of thallus water molecules escape to realize drying, but the problems of uneven microwave distribution, poor temperature consistency and the like exist, and large-scale operation cannot be realized; the spray drying is adopted, the prepared bacterial suspension is directly sprayed into mist by an atomizing device and is directly contacted with high-temperature air in a drying chamber for heat and mass transfer, the drying time is extremely short, the material temperature is low, the product dispersibility and dissolubility are good, the process is simple, the equipment cost is relatively low, and the spray drying is particularly suitable for industrial continuous production and is widely used in the fields of medicines and foods. However, the solution is fully mixed with high-temperature air in the spray drying process, so that the temperature of the bacteria liquid is sharply increased, and probiotics are killed in a large amount. Therefore, how to improve the survival rate of probiotics at high temperature is one of the current research hotspots.
Patent publication No. CN112391303A discloses a preparation process of bifidobacterium powder and probiotics thereof, which is characterized in that thalli and a matrix are mixed before spray drying, so that the heat resistance and stress resistance activity of bifidobacterium is improved, the survival rate of bifidobacterium spray drying is obviously improved, and the flowing property of the bifidobacterium powder is improved. Although the high-temperature stability of the probiotics can be improved, the acid resistance and the choline resistance of the probiotics are not improved.
In conclusion, the technical problems of poor high-temperature stability, poor acid and choline resistance and the like of the probiotic powder generally exist in the prior art.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide probiotic powder with a weight-losing effect and a preparation method thereof. The probiotic powder with the weight-losing effect has a good weight-losing effect, does not rebound after losing weight, and has a certain regulation effect on intestinal flora. In addition, the special treatment of the probiotics improves the high temperature resistance of the probiotics and improves the acid and choline resistance of the probiotics.
In order to realize the technical problem, the technical scheme of the invention is as follows:
a probiotic powder with a weight-losing effect comprises the following components in parts by weight:
50-65 parts of composite probiotics, 23-41 parts of prebiotics, 5-10 parts of fig juice, 2-5 parts of radix ophiopogonis extract, 15-26 parts of water-soluble dietary fiber, 17-24 parts of additive, 5-10 parts of propolis and 3-7 parts of protamine.
Further, the composite probiotics in the probiotic powder with the weight-reducing effect comprise one or more of lactobacillus mansonii, clostridium butyricum, streptococcus intermedius and bifield bacteria.
Furthermore, the prebiotics in the probiotic powder with the weight-losing effect consist of coriolus versicolor polysaccharide, spirulina and lactoferrin according to the mass ratio of 6-10:2-5: 13-17.
Further, the preparation method of the ophiopogon root extract in the probiotic powder with the weight-losing effect comprises the following steps: slicing dry radix Ophiopogonis root and whisker, pulverizing to 10-15um, adding 10-15 times of 75-80% ethanol water solution, reflux-extracting for 3-5 times, each time for 2-3 hr, mixing extractive solutions, and recovering ethanol to obtain radix Ophiopogonis extract.
Furthermore, the water-soluble dietary fiber in the probiotic powder with the weight-losing effect is composed of polydextrose, shaddock peel pectin and pineapple pectin according to the mass ratio of 3-5:8-11: 6-9.
Furthermore, the additive in the probiotic powder with the weight-losing effect consists of seaweed gel and sodium alginate according to the mass ratio of 2-7: 11-15.
The invention also provides a preparation method of the probiotic powder with the weight-losing effect, which comprises the following steps:
s1, fully mixing the composite probiotics with the additive, then adding the fig juice, stirring, drying and crushing to obtain powder I;
s2, fully mixing the powder I prepared in the step S1 with prebiotics, then adding the radix ophiopogonis extract, stirring, drying and crushing to obtain powder II;
s3, mixing the powder II prepared in the step S2 with water-soluble dietary fiber and protamine, adding propolis, stirring, drying, and pulverizing.
Further, in the step S1, the stirring speed is 300-500rpm, the stirring time is 10-20min, and the drying temperature is 30-35 ℃.
Further, in the step S2, the stirring speed is 300-500rpm, the stirring time is 10-20min, and the drying temperature is 30-35 ℃.
Further, in the step S3, the stirring speed is 800-.
The probiotics can be reactivated rapidly on the intestinal tract, decompose sugar in the intestinal tract, control fat synthesis, release acetic acid and lactic acid, stimulate intestinal tract peristalsis, recover intestinal tract smoothness, play a role in relaxing bowel, and discharge toxins in the body. Meanwhile, the probiotics can generate various amino acids and various digestive enzymes such as amylase, protease, lipase and the like, consume oxygen in intestinal tracts, maintain an anaerobic environment, inhibit the growth of germs and generate short-chain fatty acids, and the generated short-chain fatty acids can participate in heat metabolism and balance and help to lose weight.
The prebiotics can provide war food for the probiotics, improve the activity and gastrointestinal colonization ability of the probiotics, prolong the retention time of the probiotics, and simultaneously can stimulate the growth of beneficial intestinal flora and inhibit the activity or growth of harmful flora. The prebiotics in the formula disclosed by the invention are composed of coriolus versicolor polysaccharide, spirulina and lactoferrin according to the mass ratio of 6-10:2-5:13-17, and under the composition and mass ratio, the activity and colonization ability of probiotics in the stomach and intestine are optimal.
In the research process, the applicant finds that after probiotics enter the intestinal tract, in the process of killing harmful bacteria, the harmful bacteria release more toxins than usual in order to survive, or change metabolic pathways, and generate novel toxins to resist the probiotics, and the toxins can cause side reactions such as constipation to the body while resisting the probiotics. The applicant has found through a great deal of research that when the fig juice and the ophiopogon root extract are added into the probiotic powder with the weight-losing effect, the side reaction disappears, and therefore, the fact that the fig juice and the ophiopogon root extract are added can eliminate the toxin generated by harmful bacteria is presumed.
According to the invention, the probiotics are specially treated by adopting the alginate and the sodium alginate to achieve a capsule wrapping effect, so that the strains are in a dormant state before being eaten, the strains are stored for a long time under the conditions of high temperature and different humidity, and a large amount of activity is still maintained during rehydration, thereby improving the high temperature resistance and choline resistance of the probiotics.
The water-soluble dietary fiber can effectively activate probiotics in intestinal tracts, promote mass propagation of the probiotics and promote the effect and efficacy of the probiotics, but the water-soluble dietary fiber is directly agglomerated (hydrolyzed) in water in a guide inlet and can generate the physical phenomenon of tooth sticking caused by heat, so that the propolis is added into the formula and has viscosity, and can adhere the water-soluble dietary fiber together to prevent the water-soluble dietary fiber from agglomerating, thereby improving the mouthfeel of the probiotic powder. In addition, the propolis has stronger inoxidizability, the multi-class compounds contained in the propolis also have inhibition and sterilization effects, and the protamine has strong bacteriostatic ability in neutral and alkaline media.
The preparation method of the probiotic powder with the weight-losing effect, provided by the invention, comprises the steps of stirring at a low rotating speed, enabling the additive to effectively wrap the probiotics, so that the high-temperature resistance and the acid and choline resistance of the probiotics are improved, and stirring at a high rotating speed, so that the raw materials in the formula can be quickly and fully mixed, and the preparation period is shortened.
Compared with the prior art, the probiotic powder with the weight-losing effect and the preparation method thereof provided by the invention have the following technical advantages:
(1) the probiotic powder with the weight-losing effect has a good weight-losing effect, does not rebound after losing weight, and has a certain regulation effect on intestinal flora;
(2) the probiotic powder with the weight-losing effect provided by the invention is prepared by wrapping probiotics by adopting alginate gel and sodium alginate, so that the high temperature resistance and acid and choline resistance of the probiotics are improved;
(3) the probiotic powder with the weight-losing effect is in a dormant state before being eaten, still keeps a large amount of activity during rehydration, and has the advantages of simple preparation process, controllable conditions and easy realization of industrial production.
Detailed Description
The present invention is further described below by way of specific embodiments, but the present invention is not limited to only the following examples. Various modifications may be made by those skilled in the art based on the basic idea of the invention, but it is within the scope of the invention as long as it does not depart from the basic idea of the invention.
Example 1A probiotic powder with weight reducing effect and method for preparing the same
The probiotic powder with the weight-losing effect comprises the following components in parts by weight:
50 parts of composite probiotics, 23 parts of prebiotics, 5 parts of fig juice, 2 parts of dwarf lilyturf tuber extract, 15 parts of water-soluble dietary fiber, 17 parts of additive, 5 parts of propolis and 3 parts of protamine.
The composite probiotics comprise lactobacillus mansonii, clostridium butyricum, streptococcus intermedius and bifield bacteria according to the mass ratio of 9:4:7: 3; the prebiotics are composed of coriolus versicolor polysaccharide, spirulina and lactoferrin according to a mass ratio of 6:2: 13; the preparation method of the radix ophiopogonis extract comprises the following steps: slicing dry radix Ophiopogonis root, pulverizing to 10um, adding 10 times of 75% ethanol water solution, extracting under reflux for 2 hr for 3 times, mixing extractive solutions, and recovering ethanol to obtain radix Ophiopogonis extract; the water-soluble dietary fiber is composed of polydextrose, shaddock peel pectin and pineapple pectin according to the mass ratio of 3:8: 6; the additive is composed of alginate and sodium alginate according to a mass ratio of 2: 11.
The preparation method of the probiotic powder with the weight-losing effect comprises the following steps:
s1, fully mixing the composite probiotics with the additive, then adding the fig juice, stirring for 10min at the rotating speed of 300rpm, putting the mixture into a drying oven at the temperature of 30 ℃, drying and crushing to obtain powder I;
s2, fully mixing the powder I prepared in the step S1 with prebiotics, then adding the radix ophiopogonis extract, stirring for 10min at the rotating speed of 300rpm, then placing the mixture into an oven at the temperature of 30 ℃ for drying, and crushing to obtain powder II;
s3, fully mixing the powder II prepared in the step S2 with water-soluble dietary fibers and protamine, adding propolis, stirring at the rotating speed of 800rpm for 5min, then placing in a drying oven at 40 ℃ for drying, and crushing to obtain the product.
Example 2 probiotic powder with weight-reducing effect and preparation method thereof
The probiotic powder with the weight-losing effect comprises the following components in parts by weight:
59 parts of composite probiotics, 37 parts of prebiotics, 8 parts of fig juice, 4 parts of radix ophiopogonis extract, 23 parts of water-soluble dietary fiber, 21 parts of additive, 8 parts of propolis and 6 parts of protamine.
The composite probiotics comprise lactobacillus mansonii, clostridium butyricum, intermediate streptococcus and bifield bacteria according to the mass ratio of 9:4:7: 3; the prebiotics consist of coriolus versicolor polysaccharide, spirulina and lactoferrin according to a mass ratio of 8:4: 15; the preparation method of the radix ophiopogonis extract comprises the following steps: slicing dry radix Ophiopogonis root, pulverizing to 13um, adding 14 times of 78% ethanol water solution, reflux-extracting for 4 times (2.5 hr each time), mixing extractive solutions, and recovering ethanol to obtain radix Ophiopogonis extract; the water-soluble dietary fiber is composed of polydextrose, shaddock peel pectin and pineapple pectin according to a mass ratio of 4:10: 7; the additive is composed of alginate and sodium alginate according to a mass ratio of 5: 13.
The preparation method of the probiotic powder with the weight-losing effect comprises the following steps:
s1, fully mixing the composite probiotics with the additive, then adding the fig juice, stirring for 17min at the rotating speed of 400rpm, putting the mixture into a drying oven at 33 ℃, drying and crushing to obtain powder I;
s2, fully mixing the powder I prepared in the step S1 with prebiotics, then adding the radix ophiopogonis extract, stirring for 17min at the rotating speed of 400rpm, then placing the mixture into an oven at 33 ℃, drying and crushing to obtain powder II;
s3, mixing the powder II prepared in the step S2 with water-soluble dietary fiber and protamine thoroughly, then adding propolis, stirring at 900rpm for 7min, then placing in a 45 ℃ oven for drying, and pulverizing to obtain the final product.
Example 3A probiotic powder with weight reducing effect and its preparation method
The probiotic powder with the weight-losing effect comprises the following components in parts by weight:
65 parts of composite probiotics, 41 parts of prebiotics, 10 parts of fig juice, 5 parts of dwarf lilyturf tuber extract, 26 parts of water-soluble dietary fiber, 24 parts of additive, 10 parts of propolis and 7 parts of protamine.
The composite probiotics comprise lactobacillus mansonii, clostridium butyricum, intermediate streptococcus and bifield bacteria according to the mass ratio of 9:4:7: 3; the prebiotics are composed of coriolus versicolor polysaccharide, spirulina and lactoferrin according to a mass ratio of 10:5: 17; the preparation method of the radix ophiopogonis extract comprises the following steps: slicing dry radix Ophiopogonis root, pulverizing to 15um, adding 15 times of 80% ethanol water solution, reflux-extracting for 5 times, each time for 3 hr, mixing extractive solutions, and recovering ethanol to obtain radix Ophiopogonis extract; the water-soluble dietary fiber consists of polydextrose, shaddock peel pectin and pineapple pectin according to a mass ratio of 5:11: 9; the additive is composed of alginate and sodium alginate according to a mass ratio of 7: 15.
The preparation method of the probiotic powder with the weight-losing effect comprises the following steps:
s1, fully mixing the composite probiotics with the additive, then adding the fig juice, stirring for 20min at the rotating speed of 500rpm, putting the mixture into a drying oven at 35 ℃ for drying, and crushing to obtain powder I;
s2, fully mixing the powder I prepared in the step S1 with prebiotics, then adding the radix ophiopogonis extract, stirring at the rotating speed of 500rpm for 20min, then placing into a drying oven at 35 ℃ for drying, and crushing to obtain powder II;
s3, fully mixing the powder II prepared in the step S2 with water-soluble dietary fibers and protamine, adding propolis, stirring at the rotating speed of 1000rpm for 10min, then placing in a 50 ℃ oven for drying, and crushing to obtain the product.
Comparative example 1 probiotic powder with weight-losing effect and preparation method thereof
The formulation and preparation method of the probiotic powder with weight-reducing effect described in this comparative example are similar to those of example 2.
The comparative example differs from example 2 in that: the preparation method of the probiotic powder with the weight-losing effect in the comparative example has the stirring speed of 1000rpm in the steps S1 and S2.
Comparative example 2 probiotic powder with weight-losing effect and preparation method thereof
The formulation and preparation method of the probiotic powder with weight-reducing effect described in this comparative example are similar to those of example 2.
The comparative example differs from example 2 in that: in this comparative example, the same amount of ophiopogon root extract was used instead of fig juice.
Comparative example 3, probiotic powder with weight-losing effect and preparation method thereof
The formulation and preparation method of the probiotic powder with weight-reducing effect described in this comparative example are similar to those of example 2.
The comparative example differs from example 2 in that: in this comparative example, the same amount of fig juice was used instead of the ophiopogon root extract.
Comparative example 4 probiotic powder with weight-reducing effect and preparation method thereof
The formulation and preparation method of the probiotic powder with weight-reducing effect described in this comparative example are similar to those of example 2.
The comparative example differs from example 2 in that: all the additives described in this comparative example were alginate gels.
Comparative example 5 probiotic powder with weight-losing effect and preparation method thereof
The formulation and preparation method of the probiotic powder with weight-reducing effect described in this comparative example are similar to those of example 2.
The comparative example differs from example 2 in that: all of the additives described in this comparative example were sodium alginate.
Test example I, test of weight loss Effect
Test samples: probiotic powder with weight-losing effect prepared in examples 1-3, comparative example 1 group and comparative examples 4-5 group;
the test method comprises the following steps: 80 SHR rats (with the weight of 180-; molding feeds (base feed 39%, lard 23%, sucrose 21%, egg yolk powder 9%, and salt 8%) were given to the groups except the blank group; the blank groups are fed with common feed at a dose of 25 g/feed/day, and the groups are fed with common feed after eating, and the drinking water of each group is not limited. Wherein the test samples prepared in examples 1-3, comparative example 1 and comparative examples 4-5 were administered to groups of examples 1-3, comparative example 1 and comparative examples 4-5, respectively, at a feeding amount of 0.3g/kg, and the test samples of the blank group and the model group were administered with the same amount of distilled water, and the test samples of each group were stirred in the feed for consumption. The animals were continuously fed for 6 weeks, and the body weight of the animals was weighed at 2 weeks, 4 weeks and 6 weeks of the experiment, respectively, and the average value of the body weight of the animals in each group was recorded.
And (3) test results: the test results are shown in Table 1.
TABLE 1 test results of weight loss effect
Group of Body weight/g for 2 weeks Body weight/g for 4 weeks Body weight/g for 6 weeks
Example 1 283.7 336.2 391.5
Example 2 266.4 327.6 386.7
Example 3 276.2 331.8 389.6
Comparative example 1 308.4 356.7 411.3
Comparative example 4 293.1 348.5 406.4
Comparative example 5 299.4 350.2 410.2
Model set 314.2 365.7 416.5
Blank group 258.3 316.1 372.5
As can be seen from Table 1, the body weight of the rats in the model group increased more than that in the blank group, indicating the success of the modeling in this experiment. Compared with the model group, the weight of the rats in the groups of examples 1 to 3 is obviously reduced, which shows that the probiotic powder with the weight-reducing effect provided by the invention has a good weight-reducing effect, wherein the weight of the rats in the group of example 2 is reduced most compared with that of the model group, and the most preferred embodiment of the invention is realized.
Compared with the example 2, the rotation speed of the probiotic powder preparation method in the step S1 and the step S2 is changed in the comparative example 1, but the weight of the rat is increased compared with that of the rat in the example 2, which shows that the low rotation speed adopted in the step S1 and the step S2 can realize the coating of the additive on the probiotic and improve the acid and choline resistance of the probiotic in the stomach; the types of the additives are changed in the comparative example 4 and the comparative example 5 respectively, but the weight of the rat is increased compared with that of the rat in the example 2, which shows that the alginate and the sodium alginate both realize the coating of the probiotics in the invention, and the alginate or the sodium alginate alone can not realize the complete coating of the probiotics, so that part of the probiotics is killed by gastric acid, and the weight-losing effect is reduced.
Test example II, acid and choline resistance, high temperature resistance test
Test samples: probiotic powder having a weight-losing effect prepared in examples 1 to 3, comparative example 1 and comparative examples 4 to 5;
the test method comprises the following steps: respectively weighing 20g of the probiotic powder with the weight-losing effect prepared in the examples 1-3, the comparative example 1 and the comparative examples 4-5, respectively adding the probiotic powder into 180mL of peptone water solution with the mass concentration of 0.1%, mixing the peptone water solution at 40 ℃ for 20 minutes, uniformly oscillating, and taking 1mL of the solution to measure the viable count by a plate counting method and recording the viable count as A1. Adding pepsin into sterile physiological saline according to the proportion of 3g/L, adjusting the pH value to 3.0, uniformly mixing to obtain simulated gastric juice, respectively weighing 4g of the probiotic powder with the weight-losing effect prepared in the examples 1-3, the comparative example 1 and the comparative examples 4-5, respectively adding the probiotic powder into 300mL of simulated gastric juice, culturing at 37 ℃ for 8 hours, taking 1mL of solution, determining the number of viable bacteria according to the method, recording the number of viable bacteria as A2, and then calculating the viable bacteria survival rate: the live bacteria survival rate is A2/A1 multiplied by 100%, and the higher the live bacteria survival rate is, the better the acid and choline resistance of the probiotic composition is.
And (3) high temperature resistance test: detecting the survival rate of viable bacteria of the composite probiotic powder, placing the composite probiotic powder at 50-60 ℃ for 24h, cooling to 20 ℃, detecting the survival rate of the viable bacteria, and calculating the survival rate of the viable bacteria;
and (3) test results: the test results are shown in Table 2.
TABLE 2 test results of acid, choline and high temperature resistance
Group of Live bacteria survival rate under action of acid and choline% Live bacteria survival rate/percentage under high temperature action
Example 1 90.3 85.6
Example 2 91.0 88.1
Example 3 90.5 86.4
Comparative example 1 54.6 49.1
Comparative example 4 79.8 65.2
Comparative example 5 68.2 59.5
As can be seen from table 2, the viable bacteria survival rates of the probiotic powder with weight-reducing effect prepared in the embodiments 1 to 3 of the present invention under the action of acid and choline are 90.5 to 91.0%, and the viable bacteria survival rate under the action of high temperature is 85.6 to 88.1%, which indicates that the probiotic powder with weight-reducing effect provided by the present invention has good acid and choline resistance and high temperature resistance. In the comparative example 1, the stirring speed in the preparation process is changed, but the survival rate of the acid-resistant and choline-resistant live bacteria and the survival rate of the high-temperature-resistant live bacteria are greatly reduced, which shows that in the preparation process, the low speed can realize the effective coating of the additive on the probiotics; the types of the additives are changed in the comparative examples 4 and 5 respectively, but the survival rates of both acid-resistant and choline-resistant live bacteria and high-temperature-resistant live bacteria are greatly reduced, which fully indicates that the single seaweed gel or sodium alginate in the invention can not completely wrap the probiotics, so that the acid resistance and choline resistance of the probiotic powder prepared in the comparative examples 4 and 5 are reduced.
Test example three, side effect test
Test samples: probiotic powder with weight-reducing effect prepared in examples 1-3 and comparative examples 2-3;
the test method comprises the following steps: selecting 70 male Kunming pure white mice, weighing 20-25g, freely drinking water, feeding at room temperature for 7 days, randomly dividing into 6 groups, namely, an example 1-3 group, a comparative example 2-3 group, a control group and a blank group, after fasting for 16 hours without water prohibition, measuring the mice in the next day, and respectively feeding compound diphenoxylate suspension (5 mg/kg) into the example 1-3 group, the comparative example 2-3 group and the model group, feeding the control group with equal amount of distilled water, feeding the test samples prepared in the example 1-3 group and the comparative example 2-3 group after 60min, respectively feeding the test samples to the mice by stirring the test samples in the feed at the feeding amount of 0.3g/kg, and respectively feeding the control group and the model group with equal amount of distilled water; and after 20min, giving a carbon powder liquid indicator. The time of first pellet defecation and the weight of defecation within 2h of each animal were recorded from the beginning of ink filling (mice were not fasted and were not forbidden to drink after administration of carbon powder liquid indicator).
And (3) test results: the test results are shown in Table 3.
Table 3 side effect test results
Group of First defecation time/min Defecation amount/g within 2h
Example 1 41.9 2.91
Example 2 42.3 3.00
Practice ofExample 3 41.6 3.02
Comparative example 2 84.1 1.67
Comparative example 3 95.0 1.45
Model set 135.1 0
Blank group 42.5 3.01
As can be seen from Table 3, compared with the blank group, the initial defecation time of the mice in the model group is remarkably prolonged, and the defecation amount within 2h is greatly reduced, which indicates that the constipation model of the mice in the test is successfully modeled. Compared with a model group, the first defecation time of the mice in the groups of examples 1 to 3 is obviously shortened, the defecation time is slightly different from that of a blank group, and the defecation amount in 2 hours is increased compared with that of the model group, so that the probiotic powder with the weight-losing effect can condition intestinal tracts, improve constipation and simultaneously avoid adverse reactions such as constipation and the like caused by toxin release of harmful bacteria in the process of killing the harmful bacteria by the probiotics. In comparative example 2 and comparative example 3, only the ophiopogon root extract and the fig juice are added respectively, but the defecation time of the mice is longer than that of example 2, the defecation amount in 2h is less than that of example 2, but the defecation time is shortened than that of the model group mice, and the defecation amount in 2h is increased, so that the probiotics release more toxins in the process of killing the harmful bacteria, and the constipation of the mice is aggravated, which shows that the fig juice or the ophiopogon root extract with a single component in the formula cannot kill the toxins generated by the harmful bacteria.
The above examples are merely illustrative of the preparation process of the present invention and do not limit the invention. Those skilled in the art will recognize that changes may be made to the embodiments described above without departing from the spirit and scope of the invention. Therefore, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the technical spirit of the present invention are covered by the claims of the present invention.

Claims (10)

1. The probiotic powder with the weight-losing effect is characterized by comprising the following components in parts by weight:
50-65 parts of composite probiotics, 23-41 parts of prebiotics, 5-10 parts of fig juice, 2-5 parts of radix ophiopogonis extract, 15-26 parts of water-soluble dietary fiber, 17-24 parts of additives, 5-10 parts of propolis and 3-7 parts of protamine.
2. The probiotic powder with a weight-reducing effect according to claim 1, characterized in that the composite probiotic comprises one or more of Lactobacillus raman, Clostridium butyricum, Streptococcus intermedius, and Bifidobacterium.
3. The probiotic powder with the weight-losing effect according to claim 1, wherein the prebiotics are composed of coriolus versicolor polysaccharide, spirulina and lactoferrin in a mass ratio of 6-10:2-5: 13-17.
4. The probiotic powder with weight-losing effect according to claim 1, wherein the preparation method of the radix ophiopogonis extract comprises the following steps: slicing dry radix Ophiopogonis root and whisker, pulverizing to 10-15um, adding 10-15 times of 75-80% ethanol water solution, reflux-extracting for 3-5 times, each time for 2-3 hr, mixing extractive solutions, and recovering ethanol to obtain radix Ophiopogonis extract.
5. The probiotic powder with the weight-losing effect according to claim 1, wherein the water-soluble dietary fibers consist of polydextrose, pomelo peel pectin and pineapple pectin in a mass ratio of 3-5:8-11: 6-9.
6. The probiotic powder with the weight-losing effect according to claim 1, wherein the additive consists of alginate jelly and sodium alginate according to a mass ratio of 2-7: 11-15.
7. A method for preparing probiotic powder with slimming effect according to any of claims 1 to 6, characterized by comprising the following steps:
s1, fully mixing the composite probiotics with the additive, then adding the fig juice, stirring, drying and crushing to obtain powder I;
s2, fully mixing the powder I prepared in the step S1 with prebiotics, then adding the ophiopogon root extract, stirring, drying and crushing to obtain powder II;
s3, mixing the powder II prepared in the step S2 with water-soluble dietary fiber and protamine, adding propolis, stirring, drying, and pulverizing.
8. The method for preparing probiotic powder with weight-reducing effect as claimed in claim 7, wherein the stirring speed in step S1 is 300-500rpm, the stirring time is 10-20min, and the drying temperature is 30-35 ℃.
9. The method for preparing probiotic powder with weight-reducing effect as claimed in claim 7, wherein in the step S2, the stirring speed is 300-500rpm, the stirring time is 10-20min, and the drying temperature is 30-35 ℃.
10. The method for preparing probiotic powder with weight-reducing effect as claimed in claim 7, wherein in the step S3, the stirring speed is 800-1000rpm, the stirring time is 5-10min, and the drying temperature is 40-50 ℃.
CN202210379721.2A 2022-04-12 2022-04-12 Probiotic powder with weight-losing effect and preparation method thereof Pending CN114651985A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210379721.2A CN114651985A (en) 2022-04-12 2022-04-12 Probiotic powder with weight-losing effect and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210379721.2A CN114651985A (en) 2022-04-12 2022-04-12 Probiotic powder with weight-losing effect and preparation method thereof

Publications (1)

Publication Number Publication Date
CN114651985A true CN114651985A (en) 2022-06-24

Family

ID=82034431

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210379721.2A Pending CN114651985A (en) 2022-04-12 2022-04-12 Probiotic powder with weight-losing effect and preparation method thereof

Country Status (1)

Country Link
CN (1) CN114651985A (en)

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040175389A1 (en) * 2003-01-14 2004-09-09 Porubcan Randolph Stanley Formulations to increase in vivo survival of probiotic bacteria and extend their shelf-life
CN1846716A (en) * 2006-05-12 2006-10-18 河北农业大学 Functional bifid bacterium microcapsule
CN1884513A (en) * 2006-06-23 2006-12-27 肖宏 Probiotic double layer embed microcapsule process
CN104435283A (en) * 2014-11-17 2015-03-25 金陵科技学院 Probiotic microcapsule added with prebiotics and preparation method thereof
CN105852099A (en) * 2016-04-15 2016-08-17 邵素英 Probiotic fungus compounding functional food
CN106074621A (en) * 2016-07-26 2016-11-09 紫罗兰家纺科技股份有限公司 A kind of manufacture method of probiotic bacteria finishing agent
CN108523137A (en) * 2018-04-19 2018-09-14 江苏微康生物科技有限公司 A kind of preparation method of the probiotic granulate of acid and bile salt tolerance
CN110122589A (en) * 2019-05-29 2019-08-16 山东翌丰经贸有限公司 A kind of modulation milk powder and preparation method thereof containing lactoferrin and probiotics
CN111826324A (en) * 2020-08-05 2020-10-27 厦门惠盈动物科技有限公司 Preparation method of lactobacillus rhamnosus powder
CN112868786A (en) * 2021-01-26 2021-06-01 四川省食品发酵工业研究设计院有限公司 Ficus carica probiotic product rich in dietary fiber and preparation method thereof
CN112970990A (en) * 2018-03-22 2021-06-18 山东龙力生物科技股份有限公司 Probiotic solid beverage with weight-losing and lipid-lowering effects and preparation process thereof
CN113558246A (en) * 2021-07-21 2021-10-29 石河子大学 Symbiotic bifidobacterium composite microcapsule and preparation method thereof

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040175389A1 (en) * 2003-01-14 2004-09-09 Porubcan Randolph Stanley Formulations to increase in vivo survival of probiotic bacteria and extend their shelf-life
CN1846716A (en) * 2006-05-12 2006-10-18 河北农业大学 Functional bifid bacterium microcapsule
CN1884513A (en) * 2006-06-23 2006-12-27 肖宏 Probiotic double layer embed microcapsule process
CN104435283A (en) * 2014-11-17 2015-03-25 金陵科技学院 Probiotic microcapsule added with prebiotics and preparation method thereof
CN105852099A (en) * 2016-04-15 2016-08-17 邵素英 Probiotic fungus compounding functional food
WO2018018783A1 (en) * 2016-07-26 2018-02-01 紫罗兰家纺科技股份有限公司 Method for manufacturing probiotics finishing agent
CN106074621A (en) * 2016-07-26 2016-11-09 紫罗兰家纺科技股份有限公司 A kind of manufacture method of probiotic bacteria finishing agent
CN112970990A (en) * 2018-03-22 2021-06-18 山东龙力生物科技股份有限公司 Probiotic solid beverage with weight-losing and lipid-lowering effects and preparation process thereof
CN108523137A (en) * 2018-04-19 2018-09-14 江苏微康生物科技有限公司 A kind of preparation method of the probiotic granulate of acid and bile salt tolerance
CN110122589A (en) * 2019-05-29 2019-08-16 山东翌丰经贸有限公司 A kind of modulation milk powder and preparation method thereof containing lactoferrin and probiotics
CN111826324A (en) * 2020-08-05 2020-10-27 厦门惠盈动物科技有限公司 Preparation method of lactobacillus rhamnosus powder
CN112868786A (en) * 2021-01-26 2021-06-01 四川省食品发酵工业研究设计院有限公司 Ficus carica probiotic product rich in dietary fiber and preparation method thereof
CN113558246A (en) * 2021-07-21 2021-10-29 石河子大学 Symbiotic bifidobacterium composite microcapsule and preparation method thereof

Similar Documents

Publication Publication Date Title
CN106509439B (en) The feed addictive of substitute antibiotics preventing post-weaning diarrhea
CN107164274A (en) A kind of lactic acid bacteria composite fungicide and its preparation method and application
CN111227126A (en) Anti-diarrhea growth-promoting piglet feed additive and preparation method thereof
CN115969957B (en) Composite probiotic preparation for inflammatory bowel disease and preparation method thereof
CN108904546A (en) A kind of pair of helicobacter pylori has inhibition, the probiotic combinations preparation of killing effect and preparation method thereof
CN115944665B (en) Probiotic agent for improving intestinal flora balance and preparation method and application thereof
CN111000035A (en) Formula of compound feed and production process thereof
CN105994941A (en) Non-antibiotic feed prepared through microbial fermentation
WO2008091137A1 (en) Antibiotic feedstuff additives comprising ethanol extract of house fly larvae as active ingredient and manufacturing method thereof
CN112352874A (en) Anti-piglet diarrhea probiotic preparation and preparation method thereof
CN111264686A (en) Preparation method of antibiotic-free fermented soft-particle creep feed for suckling piglets
CN110692847A (en) Antibiotic-free mixed feed additive for rabbits
CN112617000B (en) Feed for fattening beef cattle
CN114451486A (en) Fungus enzyme compound feed additive and preparation method and application thereof
CN109198209A (en) A kind of production method and its application of fermented maize
CN112471332A (en) Antibiotic-free suckling pig nursing material containing complex enzyme and preparation method thereof
CN111972564A (en) Liquid artificial milk for relieving weaning stress of piglets and preparation method thereof
US20080044396A1 (en) Method of Raising Livestock
CN111838436A (en) Green environment-friendly antibiotic-free piglet compound feed and preparation method thereof
CN116671569A (en) Active probiotic antioxidation soft sweet and preparation method thereof
CN114651985A (en) Probiotic powder with weight-losing effect and preparation method thereof
CN110973357A (en) Slow-release type compound acidifier and preparation method thereof
CN111011624A (en) Special coconut oil-lauric acid monoglyceride microcapsule for weaned piglets
CN114344341B (en) Composite probiotic composition with intestinal tract regulating effect and preparation method and application thereof
CN110679949A (en) Weight-losing composition and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20220624