Method for inducing Australian water lily to sleep in field
Technical Field
The application belongs to the technical field of Australian water lily breeding, and particularly relates to a method for inducing Australian water lily to sleep in a field.
Background
Water lily is a generic term for plants of the genus water lily (Nymphaea) of the family water lily (Nymphaeaceae). The water lily genus can be divided into cold-resistant water lily and tropical water lily according to ecological types, and belongs to five subgenera. Among them, subgenus Anecphya (the subgenera of the post-deficient water lily), also called subgenera of the Australian water lily, is native to New Guinea island, tropical Australian region, and currently, 10 species such as N.gigantea, N.attans, N.immutabilis, etc. are found in subgenera, N.gigantea is Australian water lily which is popular among them. Australian water lily can bloom throughout the year in tropical regions, but cannot resist low temperature and overwintere in open field in temperate regions, so that two serious industrial problems exist at present: (1) In temperate zone, a large part of seedlings cannot enter dormancy through low-temperature induction, dormant balls are obtained for preservation, the seedlings can only be frozen to death, and almost half of seedlings can be lost each year or can only survive after expensive heat preservation measures are taken. (2) In tropical areas, the climate is proper, the Australian water lily is not dormant throughout the year, and no or few plants are separated, so that the asexual propagation coefficient is extremely low. Some high-end Australian water lily such as 'color-changing Australian' (N.attrans), 'Ka Ben ta ya' (N.carpentariae) and the like have the reasons of few branches, limited propagation quantity and the like, so that the price is high, single plants or 1 seed balls move hundreds of yuan to thousands of yuan, the water lily audience group is severely limited, and the expansion and industrialization and large-scale development of new varieties of Australian water lily are hindered. In addition, when selling the seedling, do not form the seedling of dormancy ball, the plant is too big, has increased transportation and surviving the degree of difficulty, if can the manual regulation dormancy form dormancy ball, more do benefit to transportation and survival. In the seeds of partial tropical water lily of the subgenera of the wide tropical water lily and the cultivars bred by the seeds as parents, the leaves of the seeds have fetal growth and propagation functions, new complete plants grow from the leaf umbilicus of the seeds, and the seeds provide possibility for breeding a large number of high-quality seedlings with stable characters in a short period. However, australian water lily does not have fetal function and can only be bred by means of dividing strains. The separated propagation is a propagation mode mainly adopted by the current Australian water lily, but the propagation coefficient is low, and the production requirement is not met. At present, dormancy balls are obtained by inducing dormancy in tropical areas and temperate areas in production, and are stored at low temperature, so that a plurality of bud points can be induced after the dormancy balls are released, and a large number of plants are separated. The method can realize safe overwintering in temperate regions and solve the problem of amplifying asexual propagation seedlings in tropical regions.
Australian water lily grows and blooms throughout the year in tropical areas such as Hainan without dormancy. To obtain more balls it is necessary to manually force the balls to sleep. Some of the currently known Australian water lily dormancy methods are too complex to operate and can be realized by means of corresponding facilities, so that the production cost is high, and the popularization and the application are not easy (for example, the Australian water lily dormancy wintering method is described as CN201911077997. X). Some low temperature, light and hormone treatments and the like can also obtain dormant balls, but the dormant rate is lower, and corresponding facilities and reagents are needed to be relied on, so that the production cost cannot be well controlled.
Disclosure of Invention
In view of the shortcomings of the prior art, the application provides a method for inducing dormancy of Australian water lily in the field.
The technical scheme of the application mainly comprises the following contents:
a method of inducing dormancy in a field of australian water lily comprising the steps of: pulling up the whole Australian water lily plant to make the whole water lily float on the water surface of the field and in a nutrition stress state until a dormancy ball is formed.
Preferably, the nutritional stress is normal fertilization before the water lily is pulled up, and no fertilization is performed after the water lily is pulled up.
Preferably, after the water lily is pulled up, the nutrition conditions of the water body are as follows: NO (NO) 3 -N 13~19mg/L,NH 4 -N 2~3mg/L,P + 2~3mg/L,K + 10~23mg/L,Ca 2+ 12~27mg/L,Mg 2+ 2~3mg/L。
Preferably, after the water lily is pulled up, the nutrition conditions of the water body are as follows: NO (NO) 3 -N 13~17mg/L、NH 4 -N 2~3mg/L、P + 2~3mg/L、K + 10~16mg/L、Ca 2+ 12~17mg/L、Mg 2+ 2~3mg/L。
Preferably, the depth of the field water body is: 50-70 cm.
Preferably, the crown width of the water lily is 1-2 m.
Preferably, the induction time is year round.
The application has the following effects:
the method realizes the field in-situ dormancy of the Australian water lily, and directly induces the Australian water lily dormancy ball in the field.
By adopting the method, the dormancy time of the Australian water lily is shorter, and the dormancy rate is up to more than 80%.
The method of the application can be implemented throughout the year without time limitation.
The method has strong controllability, labor and time saving and worry saving, is convenient and feasible, and is very suitable for field operation.
Drawings
Fig. 1 is a flow chart of the dormancy and germination accelerating operation of the water lily according to the application.
Detailed Description
In order to better understand the technical content of the present application, the following provides specific examples to further illustrate the present application.
Example 1 method of inducing dormancy of Australian Nymphaea-nutritional stress
The normal fertilization is kept before the water lily is pulled up, and the fertilization is not performed after the water lily is pulled up. The crown width of the field water lily is 1-2 m in 11 months in Hainan, and the growth vigor is good. Pulling up the whole plant of the Australian water lily, so that the whole plant of the water lily floats on the water surface (the depth of the water body is 50-70 cm) in the field and is in a nutrition stress state. The nutrition conditions of the field water body when the water lily is pulled up are as follows: NO (NO) 3 -N 13~17mg/L、NH 4 -N 2~3mg/L、P + 2~3mg/L、K + 19~23mg/L、Ca 2+ 20~27mg/L、Mg 2+ 2-3 mg/L. During the treatment of nutritional stress, australian water lily leaves gradually yellow, petioles fall off, and finally dormancy balls are formed.
Induction results: from day 11 and 27, the 30-day dormancy is 80%, and the 40-day dormancy is 100%, and the plants of all sizes are dormant.
Example 2: method for inducing dormancy of Australian water lily-nutritional stress
The normal fertilization is kept before the water lily is pulled up, and the fertilization is not performed after the water lily is pulled up. The crown width of the field water lily is 1-2 m in 11 months in Hainan, and the growth vigor is good. Pulling up the whole plant of the Australian water lily, so that the whole plant of the water lily floats on the water surface (the depth of the water body is 50-70 cm) in the field and is in a nutrition stress state. The nutrition conditions of the field water body after the water lily is pulled up are as follows: NO (NO) 3 -N 13~17mg/L、NH 4 -N 2~3mg/L、P + 2~3mg/L、K + 10~16mg/L、Ca 2+ 12~17mg/L、Mg 2+ 2-3 mg/L. During the treatment of nutritional stress, australian water lily leaves gradually yellow, petioles fall off, and finally dormancy balls are formed.
Induction results: from 11 months and 24 days, the plant type dormancy device is dormant after 30 days, and is dormant after 37 days, and the plant type dormancy device is dormant regardless of the size.
Example 3 method of inducing dormancy of Australian Nymphaea-nutritional stress
The normal fertilization is kept before the water lily is pulled up, and the fertilization is not performed after the water lily is pulled up. 5 months in Hainan, the crown width of the field water lily is 1-2 m, and the growth vigor is good. Pulling out the whole Australian water lily plantThe whole water lily is floated on the field water surface (the depth of the water body is 50-70 cm) and is in a nutrition stress state. The nutrition conditions of the field water body after the water lily is pulled up are as follows: NO (NO) 3 -N 13~19mg/L、NH 4 -N 2~3mg/L、P + 2~3mg/L、K + 20~23mg/L、Ca 2+ 19~22mg/L、Mg 2+ 2-3 mg/L. During the treatment of nutritional stress, australian water lily leaves gradually yellow, petioles fall off, and finally dormancy balls are formed.
Induction results: from day 17 of 5 months, the 40 days had been dormant 82% and the 52 days were dormant 100% and the plants were dormant regardless of size.
Comparative example 1 method of inducing dormancy of Australian water lily-hormone treatment
Water lily is normally managed, and water lily leaves are sprayed by adopting 300mg/L ethylene in 11 months.
Induction results: no dormancy occurs.
Comparative example 2 method of inducing dormancy of Australian water lily-hormone treatment
Water lily is normally managed, and 100mg/LABA (abscisic acid) is adopted to spray water lily leaves in the south of China for 11 months.
Induction results: the water lily has no change and no dormancy.
Comparative example 3 method of inducing dormancy of Australian water lily-hormone treatment
Water lily is normally managed, and water lily leaves are sprayed by 300mg/LABA (abscisic acid) in 11 months.
Induction results: the sprayed leaves turn red (a feature of dormancy), but the new leaves are unchanged and no dormancy occurs.
Comparative example 4 method of inducing dormancy of Australian water lily-oligotrophic treatment
Location: hainan. The oligotrophic treatment (fertilizing once for 6 months 1 and then not fertilizing continuously for 6 months) is carried out on the Australian water lily half a year in advance.
Induction results: plants in 9-12 months have poor flowering performance, poor disease and insect resistance, and dormancy in 12-1 month occurs, but the dormancy rate is lower (lower than 50%).
The foregoing description of the preferred embodiments of the application is not intended to limit the application to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the application are intended to be included within the scope of the application.