CN114624085A - Biological cell staining device and using method thereof - Google Patents

Biological cell staining device and using method thereof Download PDF

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Publication number
CN114624085A
CN114624085A CN202210210057.9A CN202210210057A CN114624085A CN 114624085 A CN114624085 A CN 114624085A CN 202210210057 A CN202210210057 A CN 202210210057A CN 114624085 A CN114624085 A CN 114624085A
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China
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rotating
rod
fixedly connected
enabling
test tube
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CN202210210057.9A
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Chinese (zh)
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王翔
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Wuhan Botao Biotechnology Co ltd
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Wuhan Botao Biotechnology Co ltd
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Priority to CN202210210057.9A priority Critical patent/CN114624085A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a biological cell dyeing device and a using method thereof, and the biological cell dyeing device comprises a workbench, wherein a rotating mechanism is arranged in the middle of the top of the workbench, a clamping mechanism is arranged at the top of the rotating mechanism, a hydraulic telescopic rod is fixedly connected to the right side of the top of the workbench, a connecting plate is fixedly connected to the top of the hydraulic telescopic rod, a rotating disc is fixedly connected to the bottom of the connecting plate, a plurality of injection heads are symmetrically and fixedly sleeved on the surface of the rotating disc, outer sealing plates are symmetrically and movably sleeved on the surface of the workbench, and a control console is fixedly connected to the left side of the top of the workbench. According to the biological cell dyeing device and the using method thereof, the biological cells can be dyed quickly by the back operation of inclination and reset of the clamping mechanism, and the problems that although the biological cells can be uniformly mixed in a rotating mode, the rotating time is long, and the mixing of a dyeing agent and the biological cells cannot be shortened are solved.

Description

Biological cell staining device and using method thereof
Technical Field
The invention relates to the technical field of biological cells, in particular to a biological cell staining device and a using method thereof.
Background
The most important and essential features of an organism are that the organism metabolizes and is inherited. All organisms must be anabolic and catabolic, which are two processes in opposition to each other, and can reproduce, which is the basis of life phenomena. Nature is composed of biological and non-biological matter and energy. Living organisms are called living beings, and inanimate organisms including substances and energy are called non-living organisms.
The existing biological cells rotate through equipment after dropping the coloring agent, so that the coloring agent and the biological cells are uniformly mixed, but the coloring agent and the biological cells can be uniformly mixed only in a rotating mode, but the rotating time is longer, and the mixing of the coloring agent and the biological cells cannot be shortened.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a biological cell dyeing device and a using method thereof, which solve the problems that although the biological cell dyeing device can be uniformly mixed by a rotating mode, the rotating time is longer, and the mixing of a dyeing agent and biological cells cannot be shortened.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a biological cell dyeing apparatus, includes the workstation, the centre at workstation top is provided with slewing mechanism, slewing mechanism's top is provided with fixture, the right fixedly connected with hydraulic telescoping rod at workstation top, hydraulic telescoping rod's top fixedly connected with connecting plate, the bottom fixedly connected with rolling disc of connecting plate, the fixed cover in surface symmetry of rolling disc has connect a plurality of injection heads.
Slewing mechanism includes fixed disk and dwang, the fixed cover of surface symmetry of dwang has cup jointed second bearing and first bearing, the fixed surface symmetry fixedly connected with round pin post of first bearing, the cylinder has been cup jointed through the runner assembly rotation in the surface of second bearing, the bottom fixedly connected with servo motor of dwang, servo motor's fixed cover in surface has connect the lagging.
The fixture includes upper cover and lower cover, the lead screw has been cup jointed to the equal screw thread in surface of upper cover and lower cover, a plurality of standing grooves have been seted up to the fixed surface symmetry of upper cover and dwang, a plurality of sliding tray have been seted up to the fixed top symmetry of lower cover, and is a plurality of the spout has been seted up to the fixed inner wall symmetry of sliding tray, and is a plurality of the extrusion ram has been cup jointed in the inner chamber slip of sliding tray, the fixed surface symmetry fixedly connected with slider of extrusion ram, fixedly connected with spring between the surface of slider and the inner wall of spout.
Preferably, the outer sealing plates are symmetrically and movably sleeved on the surface of the workbench, and the left side of the top of the workbench is fixedly connected with the control console.
Preferably, the top of dwang passes through mounting disc fixed mounting on the top of lower cover, fixed disk fixed connection is on the top of workstation.
Preferably, the rotating assembly is fixedly connected to the inner wall of the fixed disc, and the pin and one end of the sleeve plate are rotatably sleeved on the inner wall of the workbench.
Preferably, one end of the sliding groove is communicated with the inner cavity of the placing groove, and one end of the extrusion rod is matched with the inner wall of the placing groove.
Preferably, the upper cover and the lower cover are symmetrically and fixedly installed through bolts, and the surface of the sliding block is sleeved on the inner wall of the sliding groove in a sliding mode.
Preferably, the bottom of the screw rod is in contact with the other end of the extrusion rod, and the placing groove is aligned with the central line of the injection head.
The invention also discloses a using method of the biological cell staining device, which comprises the following steps:
s1, clamping, firstly, placing test tubes filled with biological cells in an inner cavity of a placing groove in sequence, then rotating a screw rod, enabling the screw rod to move downwards on the surfaces of an upper cover and a lower cover, enabling the bottom of the screw rod to abut against one end, opposite to an extrusion rod, of the extrusion rod to be extruded, enabling the extrusion rod to move towards one side in the inner cavity of a sliding groove, enabling one end of the extrusion rod to extend out, clamping the surface of the test tube in the inner cavity of the placing groove formed in the surface of the lower cover, meanwhile, a sliding block can slide in the inner cavity of a sliding groove along with the movement of the extrusion rod, a spring can extrude the spring, and when the test tubes need to be taken out, moving the screw rod upwards, enabling the screw rod to leave one end of the extrusion rod, and driving the extrusion rod to reset under the elastic force of the spring, so that the test tubes are not clamped, and the test tubes can be taken out;
s2, injecting a coloring agent, starting a hydraulic telescopic rod through a console, lifting the hydraulic telescopic rod, driving a rotating disc to descend through a connecting plate, inserting a plurality of injection heads sleeved on the surface of the rotating disc into the inner cavity of the clamped test tube, injecting the coloring agent into the test tube, resetting the hydraulic telescopic rod through the console, extending the injection heads out of the inner cavity of the test tube, and sequentially covering covers at the top of the test tube;
s3, it is rotatory, at last the servo motor on lagging surface is first started to the rethread control cabinet, make servo motor 'S output drive the dwang rotate in the inner chamber of first bearing and second bearing, then drive fixture through the mounting disc and rotate, it stretches out and draws back to start the cylinder of runner assembly inner chamber simultaneously to rotate again, the rotation that makes the end that stretches out of cylinder pass through runner assembly cup joints, promote the second bearing and carry out the slope of one side, simultaneously under the rotation of round pin post and lagging, thereby make the dwang drive fixture slope to 15-35 ℃, through the back operation of fixture slope and reseing, can make the test tube change the flow direction of liquid when rotating, can accelerate biological cell' S dyeing.
Advantageous effects
The invention provides a biological cell staining device and a using method thereof. Compared with the prior art, the method has the following beneficial effects:
1. this biological cell dyeing apparatus and application method, through the servo motor who starts the lagging surface, make servo motor's output drive the dwang rotate in the inner chamber of first bearing and second bearing, then drive fixture through the mounting disc and rotate, the cylinder that starts the runner assembly inner chamber when rotating again stretches out and draws back, the rotation that makes the end that stretches out of cylinder pass through runner assembly cup joints, promote the second bearing and carry out the slope of one side, simultaneously under the rotation of round pin post and lagging, thereby make the dwang drive fixture slope to 15-35 ℃, through the back operation of fixture slope and reseing, can make the test tube change the flow direction of liquid when rotating, can accelerate biological cell's dyeing.
2. This biological cell dyeing apparatus and application method thereof, put in the inner chamber of standing groove in proper order through the test tube that is equipped with biological cell, then rotate the lead screw, make the lead screw carry out the decurrent removal on the surface of upper cover and lower cover, the bottom that makes the lead screw supports and serves in the relative one of stripper bar, and extrude the stripper bar, make the stripper bar advance to the removal of one side in the inner chamber of sliding tray, make the one end of stripper bar stretch out, come to carry out the centre gripping to standing groove inner chamber test tube surface that the lower cover surface was seted up, accomplish the centre gripping of collective simultaneously, reduce the time of individual centre gripping.
3. This biological cell dyeing apparatus and application method thereof, start hydraulic telescoping rod through the control cabinet, make hydraulic telescoping rod go up and down, thereby drive the rolling disc through the connecting plate and descend, a plurality of injection heads that make the rolling disc surface cup joint insert the test tube inner chamber of centre gripping, then inject the coloring agent in the test tube, then rethread control cabinet resets hydraulic telescoping rod, make the injection head stretch out from the inner chamber of test tube, then cover the lid at test tube top in proper order, realize the effect of injection simultaneously.
Drawings
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a schematic view of the rotating mechanism of the present invention;
FIG. 3 is a schematic view of a clamping mechanism of the present invention;
FIG. 4 is an exploded view of the structural clamping mechanism of the present invention;
FIG. 5 is a partial enlarged view of the point A in FIG. 4 according to the present invention.
In the figure: 1. a work table; 2. an outer sealing plate; 3. a hydraulic telescopic rod; 4. a connecting plate; 5. rotating the disc; 6. an injection head; 7. a console; 8. a rotating mechanism; 81. fixing the disc; 82. rotating the rod; 83. a first bearing; 84. a second bearing; 85. mounting a disc; 86. a pin; 87. a servo motor; 88. sheathing; 89. a rotating assembly; 810. a cylinder; 9. a clamping mechanism; 91. an upper cover; 92. a lower cover; 93. a placement groove; 94. a bolt; 95. a screw rod; 96. a slider; 97. a chute; 98. an extrusion stem; 99. a spring; 910. a sliding groove.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, an embodiment of the present invention provides a technical solution: the utility model provides a biological cell dyeing apparatus, including workstation 1, the centre at 1 top of workstation is provided with slewing mechanism 8, slewing mechanism 8's top is provided with fixture 9, the right fixedly connected with hydraulic telescoping rod 3 at 1 top of workstation, the top fixedly connected with connecting plate 4 of hydraulic telescoping rod 3, the bottom fixedly connected with rolling disc 5 of connecting plate 4, the fixed cover in surface symmetry of rolling disc 5 has been cup jointed a plurality of injection heads 6, outer shrouding 2 has been cup jointed in the surface symmetry activity of workstation 1, the left side fixedly connected with control cabinet 7 at workstation 1's top.
Please refer to fig. 2, the rotating mechanism 8 includes a fixed disk 81 and a rotating rod 82, the surface of the rotating rod 82 is symmetrically and fixedly sleeved with a second bearing 84 and a first bearing 83, the surface of the first bearing 83 is symmetrically and fixedly connected with a pin 86, the surface of the second bearing 84 is rotatably sleeved with a cylinder 810 through a rotating component 89, the bottom of the rotating rod 82 is fixedly connected with a servo motor 87, the surface of the servo motor 87 is fixedly sleeved with a sleeve plate 88, the top of the rotating rod 82 is fixedly installed on the top of the lower cover 92 through a mounting disk 85, the fixed disk 81 is fixedly connected on the top of the workbench 1, the rotating component 89 is fixedly connected on the inner wall of the fixed disk 81, one end of the pin 86 and one end of the sleeve plate 88 are rotatably sleeved on the inner wall of the workbench 1.
Referring to fig. 3-5, the clamping mechanism 9 includes an upper cover 91 and a lower cover 92, a screw 95 is screwed on the surfaces of the upper cover 91 and the lower cover 92, a plurality of placing grooves 93 are symmetrically and fixedly formed on the surfaces of the upper cover 91 and the rotating rod 82, a plurality of sliding grooves 910 are symmetrically and fixedly formed on the top of the lower cover 92, sliding grooves 97 are symmetrically and fixedly formed on the inner walls of the sliding grooves 910, an extruding rod 98 is slidingly sleeved on the inner cavity of the sliding grooves 910, sliders 96 are symmetrically and fixedly connected to the surface of the extruding rod 98, springs 99 are fixedly connected between the surfaces of the sliders 96 and the inner walls of the sliding grooves 97, one end of the sliding groove 910 is communicated with the inner cavity of the placing groove 93, one end of the extruding rod 98 is engaged with the inner wall of the placing groove 93, the upper cover 91 and the rotating rod 82 are symmetrically and fixedly mounted by bolts 94, the surface of the slider 96 is slidingly sleeved on the inner wall of the sliding groove 97, the bottom of the screw 95 is contacted with the other end of the extruding rod 98, the placement groove 93 is aligned with the centre line of the injection head 6.
The embodiment of the invention provides a technical scheme that: a using method of a biological cell staining device specifically comprises the following steps:
s1, clamping, firstly, placing the test tubes with the biological cells in the inner cavity of the placing groove 93 in sequence, then, the screw 95 is rotated to move the screw 95 downward on the surfaces of the upper cover 91 and the lower cover 92, the bottom of the screw 95 is abutted against the opposite end of the pressing bar 98, and the extrusion rod 98 is extruded to make the extrusion rod 98 move to one side in the inner cavity of the sliding groove 910, so that one end of the extrusion rod 98 extends out to clamp the surface of the test tube in the inner cavity of the placing groove 93 arranged on the surface of the lower cover 92, at the same time, the sliding block 96 will slide in the inner cavity of the sliding groove 97 along with the movement of the pressing rod 98, and the spring 99 will be pressed, in addition, when the screw rod 95 needs to be taken out, the screw rod 95 is moved upwards, so that the screw rod 95 is separated from one end of the extrusion rod 98, under the elastic force of the spring 99, the extrusion rod 98 is driven to reset, so that the test tube is not clamped and can be taken out;
s2, injecting a coloring agent, starting a hydraulic telescopic rod 3 through a control console 7, lifting the hydraulic telescopic rod 3, driving a rotating disc 5 to descend through a connecting plate 4, inserting a plurality of injection heads 6 sleeved on the surface of the rotating disc 5 into the inner cavity of the clamped test tube, injecting the coloring agent into the test tube, resetting the hydraulic telescopic rod 3 through the control console 7, extending the injection heads 6 out of the inner cavity of the test tube, and sequentially covering a cover at the top of the test tube;
s3, rotating, and finally starting the servo motor 87 on the surface of the sleeve plate 88 through the console 7, so that the output end of the servo motor 87 drives the rotating rod 82 to rotate in the inner cavities of the first bearing 83 and the second bearing 84, then driving the clamping mechanism 9 to rotate through the mounting disc 85, then starting the air cylinder 810 in the inner cavity of the rotating assembly 89 to stretch and retract while rotating, so that the extending end of the air cylinder 810 is sleeved through the rotation of the rotating assembly 89, the second bearing 84 is pushed to incline to one side, and simultaneously under the rotation of the pin 86 and the sleeve plate 88, the rotating rod 82 drives the clamping mechanism 9 to incline to 15-35 ℃, and through the inclining and resetting return operation of the clamping mechanism 9, the flowing direction of liquid can be changed when the test tube rotates, and the dyeing of biological cells can be accelerated.
Further, the top of the workbench 1 is provided with a through hole, then the rotating rod 82 is located in the through hole, the bottom of the through hole is fixedly connected with a baffle, and then the sleeve plate 88 and the pin column 86 rotate to be sleeved on the baffle.
And those not described in detail in this specification are well within the skill of those in the art.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. A biological cell staining device and a using method thereof comprise a workbench (1), and are characterized in that: a rotating mechanism (8) is arranged in the middle of the top of the workbench (1), a clamping mechanism (9) is arranged at the top of the rotating mechanism (8), a hydraulic telescopic rod (3) is fixedly connected to the right of the top of the workbench (1), a connecting plate (4) is fixedly connected to the top of the hydraulic telescopic rod (3), a rotating disc (5) is fixedly connected to the bottom of the connecting plate (4), and a plurality of injection heads (6) are symmetrically and fixedly sleeved on the surface of the rotating disc (5);
the rotating mechanism (8) comprises a fixed disc (81) and a rotating disc (82), a second bearing (84) and a first bearing (83) are symmetrically and fixedly sleeved on the surface of the rotating disc (82), a pin column (86) is symmetrically and fixedly connected on the surface of the first bearing (83), an air cylinder (810) is rotatably sleeved on the surface of the second bearing (84) through a rotating assembly (89), a servo motor (87) is fixedly connected to the bottom of the rotating disc (82), and a sleeve plate (88) is fixedly sleeved on the surface of the servo motor (87);
fixture (9) are including upper cover (91) and lower cover (92), lead screw (95) have been cup jointed to the equal screw thread in surface of upper cover (91) and lower cover (92), a plurality of standing groove (93) have been seted up to the fixed surface symmetry of upper cover (91) and dwang (82), a plurality of sliding tray (910) have been seted up to the top symmetry of lower cover (92) is fixed, and is a plurality of spout (97) have been seted up to the inner wall symmetry of sliding tray (910) is fixed, and is a plurality of extrusion pole (98) have been cup jointed in the inner chamber slip of sliding tray (910), the surface symmetry fixedly connected with slider (96) of extrusion pole (98), fixedly connected with spring (99) between the surface of slider (96) and the inner wall of spout (97).
2. The device as claimed in claim 1, wherein the device comprises: the surface symmetry activity of workstation (1) has cup jointed outer shrouding (2), the left side fixedly connected with control cabinet (7) at the top of workstation (1).
3. The device as claimed in claim 1, wherein the device comprises: the top of dwang (82) passes through mounting disc (85) fixed mounting on the top of lower cover (92), fixed disk (81) fixed connection is on the top of workstation (1).
4. The device as claimed in claim 1, wherein the device comprises: the rotating assembly (89) is fixedly connected to the inner wall of the fixed disc (81), and the pin column (86) and one end of the sleeve plate (88) are rotatably sleeved on the inner wall of the workbench (1).
5. The device as claimed in claim 1, wherein the device comprises: one end of the sliding groove (910) is communicated with the inner cavity of the placing groove (93), and one end of the extrusion rod (98) is matched with the inner wall of the placing groove (93).
6. The device as claimed in claim 1, wherein the device comprises: the upper cover (91) and the lower cover (92) are symmetrically and fixedly installed through bolts (94), and the surface of the sliding block (96) is sleeved on the inner wall of the sliding groove (97) in a sliding mode.
7. The device as claimed in claim 1, wherein the device comprises: the bottom of the screw rod (95) is contacted with the other end of the extrusion rod (98), and the placing groove (93) is aligned with the central line of the injection head (6).
8. A method for using the apparatus for staining biological cells according to any one of claims 1 to 7, wherein: the method specifically comprises the following steps:
s1, clamping, namely, sequentially placing test tubes filled with biological cells in an inner cavity of a placing groove (93), then rotating a screw rod (95) to enable the screw rod (95) to move downwards on the surfaces of an upper cover (91) and a lower cover (92), enabling the bottom of the screw rod (95) to be abutted against one end opposite to an extrusion rod (98), extruding the extrusion rod (98), enabling the extrusion rod (98) to move towards one side in the inner cavity of a sliding groove (910), enabling one end of the extrusion rod (98) to extend out, clamping the surface of the test tube in the inner cavity of the placing groove (93) formed in the surface of the lower cover (92), simultaneously enabling a sliding block (96) to slide in the inner cavity of the sliding groove (97) along with the movement of the extrusion rod (98), enabling a spring (99) to extrude, and when the test tubes need to be taken out, moving the screw rod (95) upwards to enable the screw rod (95) to be separated from one end of the extrusion rod (98), under the elastic force of the spring (99), the extrusion rod (98) is driven to reset, so that the test tube is not clamped and can be taken out;
s2, injecting a coloring agent, starting a hydraulic telescopic rod (3) through a control console (7), lifting the hydraulic telescopic rod (3), driving a rotating disc (5) to descend through a connecting plate (4), inserting a plurality of injection heads (6) sleeved on the surface of the rotating disc (5) into the inner cavity of the test tube for clamping, injecting the coloring agent into the test tube, resetting the hydraulic telescopic rod (3) through the control console (7), extending the injection heads (6) out of the inner cavity of the test tube, and sequentially covering the cover at the top of the test tube;
s3, rotating, finally, starting a servo motor (87) on the surface of a sleeve plate (88) through a console (7), enabling the output end of the servo motor (87) to drive a rotating rod (82) to rotate in the inner cavities of a first bearing (83) and a second bearing (84), then driving a clamping mechanism (9) to rotate through a mounting disc (85), starting an air cylinder (810) in the inner cavity of a rotating assembly (89) to stretch and retract while rotating, enabling the extending end of the air cylinder (810) to be sleeved through the rotation of the rotating assembly (89), pushing the second bearing (84) to incline at one side, enabling the rotating rod (82) to drive the clamping mechanism (9) to incline to 15-35 ℃ under the rotation of a pin column (86) and the sleeve plate (88), and enabling the flowing direction of liquid to be changed when a test tube rotates through the inclining and returning operations of the clamping mechanism (9), can accelerate the staining of biological cells.
CN202210210057.9A 2022-03-04 2022-03-04 Biological cell staining device and using method thereof Pending CN114624085A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210210057.9A CN114624085A (en) 2022-03-04 2022-03-04 Biological cell staining device and using method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210210057.9A CN114624085A (en) 2022-03-04 2022-03-04 Biological cell staining device and using method thereof

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CN114624085A true CN114624085A (en) 2022-06-14

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114865250A (en) * 2022-06-18 2022-08-05 河北零点新能源科技有限公司 Lithium battery polymer injection device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114865250A (en) * 2022-06-18 2022-08-05 河北零点新能源科技有限公司 Lithium battery polymer injection device
CN114865250B (en) * 2022-06-18 2023-10-31 河北零点新能源科技有限公司 Polymer injection device for lithium battery

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