CN114604871A

CN114604871A - Two-dimensional layered material MXene oriented medium and application thereof

Info

Publication number: CN114604871A
Application number: CN202210271967.8A
Authority: CN
Inventors: 秦四勇; 赵优; 张爱清
Original assignee: South Central University for Nationalities
Current assignee: South Central Minzu University
Priority date: 2022-03-18
Filing date: 2022-03-18
Publication date: 2022-06-10
Anticipated expiration: 2042-03-18
Also published as: CN114604871B

Abstract

The invention provides a two-dimensional layered material MXene oriented medium and application thereof, wherein the structural formula of the two-dimensional layered material MXene is Ti_aC_bT_XT is O, F, N with hydrophilicity, and when a is 3, b is 2, when a is 2, b is 1; placing MXene in H₂And the construction of a novel directional medium can be realized by ultrasonic dispersion in O. The MXene oriented medium can effectively orient biomolecules, can realize accurate measurement of Residual Dipole Coupling (RDCs) of the biomolecules and is used for structure analysis. Because MXene material still has orientation ability after freeze-drying and redispersion, long-term storage and use of the material can be realized. As a directional medium, the method has the characteristic of no background residual signal, and avoids the interference of the background signal on the atlas analysis. In addition, MXene materials are various in types, and the surface of the MXene materials contains a large number of active sites, so that surface chemical modification is facilitated, a foundation is laid for constructing directional media of different solvent systems for determination of anisotropic nuclear magnetic parameters, and the MXene materials have good application prospects.

Description

Two-dimensional layered material MXene oriented medium and application thereof

Technical Field

The invention relates to the technical field of nano materials, in particular to a two-dimensional layered material MXene oriented medium and application thereof.

Background

Molecular Residual Quadrupole Coupling (RQC) and Residual Dipole Coupling (RDC) in anisotropic parameters of nuclear magnetic resonance have unique application advantages for the analysis of organic molecular configuration and dominant conformation. When nuclear magnetic resonance spectroscopy (NMR) is used to measure anisotropic nuclear magnetic parameters, an orientation medium is usually introduced to create an anisotropic environment for the molecules to be measured, so that the molecules to be measured have appropriate orientation properties. Therefore, the preparation of the oriented medium with excellent performance has important significance for obtaining the anisotropic parameters of the organic molecules. Alignment media for the determination of anisotropy parameters of organic compounds fall mainly into two categories, one being stretchable or compressible polymer gels and the other being lyotropic liquid crystals.

In recent years, with the development and utilization of new alignment media, the anisotropy parameter has become one of the tools for analyzing the structure of small organic molecules. The development of the directional medium has great significance for the development of medicaments and the research of natural products. Based on the problems of complicated preparation process, background interference and the like of the existing directional medium, the development of a directional medium with more excellent performance for organic molecular structure identification is urgently needed. MXenes, a metal carbide or nitride material with a two-dimensional layer structure, has attracted a great deal of attention since its unique characteristics and various application prospects since its discovery in 2011. In recent years, researchers have found that MXenes exhibit liquid crystal behavior when they reach a certain concentration. Based on the characteristics of simple MXenes preparation, spontaneous directional arrangement of molecules, less hydrogen atom containing structure, no background interference and the like, the MXenes material can be constructed as a directional medium for analyzing the organic molecular structure.

Disclosure of Invention

In order to solve the technical problems, the invention provides a Ti-based alloy₃C₂T_xThe MXene oriented medium is constructed and applied to the field of nuclear magnetism, and the aim is to provide a two-dimensional layered material which can be applied to H₂Dispersing in O to realize the construction of novel directional medium. The MXene dispersion liquid has good fluidity and orientation, and can be used as an orientation medium for application in biomolecule RDCAnd (4) accurate measurement of s. The oriented medium can be dispersed in water, and has the characteristics of no background and adjustable size, so that the oriented medium system effectively simplifies the analytic process based on nuclear magnetic detection, and the MXene has the advantages of adjustability, controllability, large amount of active sites on the surface, and good application prospect.

According to one aspect of the invention, a two-dimensional layered material MXene oriented medium is provided, wherein the structural formula of the two-dimensional layered material MXene is Ti_aC_bT_xT is O, F, N having hydrophilicity, and when a is 3, b is 2; when a is 2, b is 1; the oriented medium is formed by enabling the two-dimensional layered material MXene to be in H₂Forming a dispersion with a concentration of 0.1 wt% to 5 wt% in O for measurement of anisotropic nuclear magnetic parameters.

Preferably, based on the above scheme, the orientation ability of the orientation medium is determined by the aspect ratio and the concentration of the dispersion liquid.

On the basis of the scheme, preferably, the two-dimensional layered material MXene is added into water after being freeze-dried, and can quickly generate orientation capacity after being ultrasonically redispersed.

On the basis of the scheme, preferably, when the concentration of the two-dimensional layered material MXene is 0.1-5 wt%, the oriented medium has 10% D₂Deuterium resonance in O solution shows residual quadrupole coupling values (RQCs) of 0-71 Hz.

On the basis of the scheme, the two-dimensional layered material MXene is an inorganic layered material and has no C-H coupling signal in an NMR spectrum.

The invention also provides an application of the two-dimensional layered material MXene orientation medium, wherein the two-dimensional layered material MXene orientation medium can induce biomolecules to generate orientation and is used for measuring molecular RDCs and analyzing structures.

On the basis of the scheme, preferably, the two-dimensional layered material MXene oriented medium can be used for measuring RDCs of water-soluble biomolecules to obtain structural information of the RDCs.

On the basis of the scheme, the two-dimensional layered material MXene oriented medium can measure RDCs of L-proline to obtain the proline structure.

On the basis of the scheme, preferably, the two-dimensional layered material MXene oriented medium can measure the RDCs of the sucrose to obtain the structure of the sucrose.

Preferably, on the basis of the scheme, MXene is Ti₃C₂T_x、Ti₂CT_xOr Ti₃CNT_xOne of (1) and (b).

The invention has the following beneficial effects:

1. the oriented medium is constructed by ultrasonic dispersion of MXene in water, wherein the MXene is formed by Ti₃AlC₂The two-dimensional nano material obtained by etching the Al layer does not contain organic carbon hydrogen, and the material can be used for obtaining a solid sample which is easier to store through freeze-drying and simultaneously keeping the original performance. 0.5 wt% MXene in H₂The resonance of deuterium in O showed RQC at 4Hz, and RQC at 5 wt% was 71 Hz. The low critical concentration, high RQC, indicates that MXene has highly oriented property as an oriented medium, and is an excellent ordered medium for measuring the biological molecules RDCs.

2. The MXene sheet material prepared by the method does not contain organic carbon-hydrogen bonds in the structure, so that the problem of difficult analysis caused by complex signals of an oriented medium in a spectrogram is solved.

3. The MXene prepared by the method can further realize the regulation and control of the orientation capability of the oriented medium by changing the ultrasonic time regulation and control size.

4. MXene prepared by the invention contains Ti₃C₂T_xBut not limited to, Ti is obtained by further using the homologous family as an etching object₂CT_x、Ti₃MXene such as CN as orientation medium.

Drawings

Fig. 1 is a flow diagram of the MXene preparation of the present invention;

FIG. 2 shows MXene colloid of the present invention in H₂The tyndall phenomenon of O dispersion;

fig. 3 shows Dynamic Light Scattering (DLS) spectra of MXene prepared in example 3 of the present invention at different ultrasound times: a)0min, b)30min, c)120min, d)300 min;

FIG. 4 shows MXene as Ti prepared in inventive example 1₃C₂T_xMXene Zeta potential analysis table;

FIG. 5 is MXene Ti prepared in inventive example 1₃C₂T_xMXene X-ray diffraction (XRD) pattern;

FIG. 6a shows MXene as Ti prepared in inventive example 1₃C₂T_xMXene X-ray photoelectron spectrum;

FIG. 6b Ti prepared in example 1 of the present invention₃C₂T_xMXene Atomic Force Microscope (AFM) images;

FIG. 6c Ti prepared according to example 1 of the present invention₃C₂T_xMXene Transmission Electron Microscope (TEM) image;

FIG. 6d Ti prepared according to inventive example 1₃C₂T_xSelected Area Electron Diffraction (SAED) pattern of MXene,

FIG. 6e Ti prepared according to example 1 of the present invention₃C₂T_xHigh power TEM images of MXene;

FIG. 7 shows Ti prepared in example 1 of the present invention₃C₂T_xFESEM images of MXene under different ultrasonic times;

FIG. 8a is a graph showing Ti at 0min for the ultrasonic treatment time prepared in example 1 of the present invention₃C₂T_xA polarization diagram of MXene in the dispersion;

FIG. 8b shows Ti at a sonication time of 30min for the preparation of example 1 according to the present invention₃C₂T_xA polarization diagram of MXene in the dispersion;

FIG. 8c is a graph of Ti content at 120min for sonication prepared in example 1 of the present invention₃C₂T_xA polarization diagram of MXene in the dispersion;

FIG. 8d shows Ti at 300min for the sonication time prepared in example 1 of the present invention₃C₂T_xA polarization diagram of MXene in the dispersion;

FIG. 9a shows MXene Ti concentrations of 0.1%, 0.5%, and 2% prepared in example 1 of the present invention₃C₂T_xViscosity of the dispersion as a function of shear rate (a);

FIG. 9b is a schematic representation of the preparation of Ti in example 1 of the present invention₃C₂T_xPlots of elasticity (G') and viscous modulus (G ") versus frequency for MXene dispersions;

FIG. 9c shows Ti prepared in example 1 of the present invention₃C₂T_xPlots of elasticity (G') and viscous modulus (G ") versus frequency for MXene dispersions;

FIG. 9d shows preparation of Ti in example 1 of the present invention₃C₂T_xPlots of elasticity (G') and viscous modulus (G ") versus frequency for MXene dispersions;

FIG. 10 shows Ti prepared in accordance with the present invention in accordance with example 1 of the present invention with different sonication times₃C₂T_xDeuterium of MXene: (²H) NMR chart;

FIG. 11a shows Ti concentrations of 30min in the ultrasonic treatment of example 1 of the present invention₃C₂T_xDeuterium of MXene: (²H) NMR chart;

FIG. 11b shows a concentration of 5% Ti prepared in example 1 of the present invention₃C₂T_xMXene hydrogen spectrogram;

FIG. 11c is a graph showing Ti concentration at 30min of sonication time for preparation prepared in example 1 of the present invention₃C₂T_xDeuterium of MXene with time: (²H) NMR chart;

FIG. 12 shows 10mg proline dissolved in Ti prepared in example 1 of the present invention₃C₂T_xMXene (concentration 20.0mg/mL)²A HNMR map;

FIG. 13 is a value of proline recorded using the MXene dispersion prepared in example 1 of the present invention as an ordered medium¹H，¹³C]-CLIP-HSQC spectrum;

fig. 14 is a graph of the fitted linear relationship of the RDCs of proline measured using the MXene dispersion prepared in example 1 of the present invention as an ordered medium and the calculated RDCs;

FIG. 15 shows 10mg of sucrose dissolved in Ti prepared in example 1 of the present invention₃C₂T_xMXene (concentration 20.0mg/mL)²A HNMR map;

FIG. 16 is a [ solution of ] sucrose recorded using MXene dispersion prepared in example 1 of the present invention as an ordered medium¹H，¹³C]-JSB-HSQC spectra;

fig. 17 is a graph of the fitted linear relationship of the RDCs of sucrose measured using the MXene dispersion prepared in example 1 of the present invention as an ordered medium and the calculated RDCs.

Detailed Description

The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

Example 1

The meaning of the two-dimensional material MXene is that M is metal titanium with different valence states, X is carbon-ene refers to alkene substances, wherein the structural formula of MXene is Ti_aC_bT_xAnd when a is 3, b is 2, when a is 2, b is 1, T is O, F, N with better hydrophilicity.

A common MXene is Ti₃C₂T_x、Ti₂CT_x、Ti₃CNT_xThe MXene dispersion liquid is prepared by adopting an in-situ lithium ion intercalation method, a hydrogen fluoride-free system of lithium fluoride and hydrochloric acid is adopted for etching, and the phase A is etched and ultrasonically stripped to obtain the MXene dispersion liquid.

Referring to fig. 1, the two-dimensional material MXene of the present invention is prepared by the following steps:

s1, placing LiF in a polytetrafluoroethylene substrate, adding concentrated hydrochloric acid, stirring for dissolving, and preparing HF;

s2, adding MAX into the polytetrafluoroethylene substrate, heating to 45 ℃, stirring, and starting an etching process;

s3, after the etching is finished, transferring the reaction liquid into a centrifuge tube, and centrifugally washing the reaction liquid to be neutral to obtain MXene dispersion liquid;

s4, transferring the MXene dispersion liquid into a glass bottle, and introducing inert gas for protection;

s5, placing the dispersion liquid obtained in the step S4 in an ultrasonic machine for ultrasonic stripping for 0-300 min;

and S6, transferring the liquid in the glass bottle to a centrifuge tube, centrifuging for 30min at 2500r/min, and then obtaining a solid by adopting a freeze-drying technology.

S7, wherein MAX comprises Ti₃AlC₂、Ti₃AlCN、Ti₂AlC。

The MXene size can be regulated and controlled by different ultrasonic etching times, and the ultrasonic time is respectively 0min, 30min, 120min and 300 min.

And in order to verify the structural information of MXene, the invention can also detect the structural information of MXene through X-ray diffraction (XRD). The results are shown in FIG. 5, the abscissa indicates the angle, the ordinate indicates the intensity of the diffraction peak of the crystal, the diffraction peak in the vicinity of 40 degrees disappears, and the 002 peak shifts to a low angle, indicating that Ti is present₃AlC₂Is successfully etched into Ti₃C₂T_xMaterial, and Ti₃C₂T_xThe interlayer spacing of (2) becomes large. By comparing the spectrum of the MAX phase and the MXene material, the change of the interlayer spacing of 5.04 angstroms can be calculated according to the Sheer formula, and the increase of the interlayer spacing further indicates the successful etching and the successful intercalation of water molecules.

Further, the element composition of MXene was analyzed by XPS, and the analysis results are shown in FIG. 6a, the abscissa represents bond energy and the ordinate represents strength.

In order to preliminarily study whether the MXene can obtain a relatively uniform nano size under the etching condition, the dimension of the MXene under different ultrasonic times is evaluated by DLS, and as can be known from the attached drawings, when the ultrasonic time is within the range of 0-300min, the dimension of the material related to the MXene can maximally reach the micron level, as shown in FIGS. 3a-d, the material with the dimension of about 200nm can be obtained along with the extension of the ultrasonic time, and the combination of scanning electron microscope images in FIGS. 7a-d can also show that the sheet dimension of the MXene is gradually reduced along with the extension of the ultrasonic time. Further, the size of the material obtained by the invention can be further regulated and controlled by changing the ultrasonic time.

Further, the method can be used for preparing a novel materialThe invention also discusses the charging property of the MXene dispersion liquid through Zeta potential, as shown in figure 4, the potential of MXene under different ultrasonic time is about-30 mV, which shows that the MXene surface contains a large amount of negative charges, and the surface contains-OH and-O combined with XPS spectrogram analysis^-And so on, and thus can form hydrogen bonds with water to obtain a stable dispersion, while obtaining a more negative potential through negative electrical repulsion.

Wherein MXene is in H₂Having liquid crystal behavior in O is shown in FIGS. 8 a-d.

The invention also provides a two-dimensional layered material MXene oriented medium, wherein the oriented medium is prepared by arranging the two-dimensional layered material MXene in H₂Forming a dispersion with a concentration of 0.1 wt% to 5 wt% in O for measurement of anisotropic nuclear magnetic parameters.

Notably, the two-dimensional layered material is in H₂O shows lyotropic liquid crystal behavior, 90mg/mL MXenes material of different sizes dispersed in water was dropped onto a glass slide, and then another glass slide was pressed over the sample and observed for birefringence by a polarizing microscope. As can be seen from fig. 8a-d, larger sized MXene has stronger birefringence while the dispersion concentration remains the same.

In addition, FIG. 9a shows 0.1 wt% Ti₃C₂T_xThe viscosity of MXene is only 0.01Pa.s, and although the viscosity of MXene dispersion increases with the concentration of the MXene dispersion, the MXene dispersion has the characteristic of shear thinning, and the shear rate increases to 100s^-1When the viscosity is reduced to 0.001Pa.s, the MXene dispersion liquid has better fluidity. Further study on its visco-elastic behavior to investigate Ti concentration at different concentrations₃C₂T_xMXene interaction. According to Ti in FIGS. 9b-d₃C₂T_xCompared with the scanning frequency, the elasticity (G ') modulus and the viscosity (G') modulus of MXene are increased in three groups along with the increase of the scanning frequency. Also, the advantage of G' over G "was observed throughout the scanning frequency range and all concentrations, indicating the formation of a gel. Further description of the inventionBy Ti₃C₂T_xThe MXene sheets have interaction force but can still keep better fluidity.

It has been found through research that such ordered mediators can direct biomolecules and limit their molecular motion, which has the potential to act as directed mediator capture molecules for RDCs. To further demonstrate the potential of MXene solutions as directing media by deuterium: (²H) NMR confirmed the ordered arrangement.

As shown in fig. 10a-d, at 600MHz ((m))¹H) In the spectrometer, 2 wt% MXene at D under different ultrasonic time is studied₂Deuterium resonance in O solution shows residual quadrupole coupling values in the range of 0-18 Hz. Further investigating the concentration dependence, FIG. 11a shows that 0.5 wt% MXene is in D₂Deuterium resonance in O solution showed a residual quadrupole coupling value of 4.2Hz, indicating that the two-dimensional layered material in solution has a certain orientation. And MXene was observed at D₂The RQCs value of O is positively correlated with its concentration. When the MXene concentration was 5 wt%, it was RQC of 71.0Hz, as shown in FIG. 11a, where the abscissa of FIG. 11a and FIG. 11b represents frequency and the ordinate represents relative intensity. Meanwhile, the time stability of the compound is researched, and FIG. 11c shows that MXene deuterium resonance with the concentration of 2 wt% in a certain time shows the residual quadrupole coupling value of 4.2Hz, so that the compound has good time stability and is used in nuclear magnetism²The signal of the H quadrupole splitting is highly symmetric with a half-width of 0.05Hz, and a relatively narrow half-width indicates less viscous from the side. MXene exhibits excellent ordering and flowability after dispersion in water, which indicates the great potential that MXene has shown as an ideal ordered medium in the measurement of biomolecule RDCs.

Example 2

To study MXene as an oriented Medium in D₂The applicability of RDCs was accurately obtained in O, and studies using proline as a model molecule are performed below.

Wherein the proline has the structural formula shown as the following formula (II), and the spectrum of C-13 is given in Table 1.

As shown in FIG. 11, 10.0mg of proline was dissolved in MXene dispersion (concentration 20.0mg/mL), which was²The H NMR spectrum showed RQC to be 22.50 Hz. In fig. 12, the abscissa represents frequency and the ordinate represents relative intensity. Proline [ 2 ] using MXene dispersion as ordered medium¹H，¹³C]CLIP-HSQC spectra and in isothermal conditions pure D₂O was used as a control, as shown in fig. 13, in which the abscissa represents the hydrogen spectrum and the ordinate represents the carbon spectrum. According to the analysis of the experimental result, all the C-H coupling on the proline skeleton can be detected in the ordered MXene oriented medium, which shows that the MXene oriented medium has good compatibility with proline. The RDCs for proline range in size from-1.4 to 37.0Hz (Table 1).

To test the accuracy of these RDCs, the present invention performed theoretical calculations using the Singular Value Decomposition (SVD) method and the program MSpin 45. Density Functional Theory (DFT) at the level of B3LYP/6-31G (d) was introduced for calculation of theoretical RDCs. The linear relationship between the measured RDCs and the Calculated RDCs is shown in FIG. 14, wherein in FIG. 14, Experimental RDCs means Experimental RDCs, scaled RDCs means Calculated RDCs, and proline means proline. Proline has a Q factor of 0.093, indicating that MXene can be an excellent medium for accurate measurement of RDCs.

Example 3

To study MXene as an oriented Medium in D₂The applicability of RDCs was accurately obtained in O, and studies using sucrose as a model molecule were conducted below.

Sucrose was used to further demonstrate the compatibility of MXene as an orienting medium. As shown in FIG. 14, 10.0mg of sucrose was dissolved in MXene dispersion(concentration: 20.0mg/mL), which²The H NMR spectrum showed RQC at 25.39 Hz. In fig. 15, the abscissa represents frequency, and the ordinate represents relative intensity. Use of MXene as an ordered Medium for the recording of sucrose¹H，¹³C]JSB-HSQC spectra, and pure D under isothermal conditions₂O was used as a control, and the experimental results are shown in FIG. 15, in which the C-13 spectrum is shown in Table 2, and in FIG. 16, the abscissa represents the hydrogen spectrum and the ordinate represents the carbon spectrum. The results show that all C-H couplings on the sucrose backbone can be detected in the ordered MXene directing medium, indicating that the MXene directing medium has good compatibility with sucrose as well. The RDCs of the sucrose detected were-7.63-18.02 Hz (Table 2). The Q factor value obtained by fitting is 0.13, and a low Q factor value indicates that the medium can be used for effectively and accurately measuring the RDC value of the sucrose, please refer to FIG. 17, wherein the abscissa represents the experimental RDCs value, and the ordinate represents the theoretical RDCs value.

¹³C positioning	δ_Cppm	¹H positioning	δ_Hppm	¹J_CH	¹T_CH	¹D_CH	¹D_CHCalculated value
								C1	72.29	H1	3.72	144.31	162.33	18.02	17.56
C2	69.07	H2	3.34	144.36	160.34	15.98	15.71
								C3	72.46	H3	3.63	145.11	162.5	17.39	16.99
C4	70.95	H4	3.43	137.3	161.61	17.29	17.47
								C5	92.08	H5	5.28	169.77	182.46	12.69	12.42
C9	76.24	H9	4.09	144.17	140.91	-3.26	-3.82
								C10	73.84	H10	3.92	144.39	143.73	-0.66	-1.14
C11	81.27	H11	3.76	149.09	141.46	-7.63	-7.90
								C13	61.15	H13a，b	3.54	144.37	148.24	3.51	5.67
C18	62.24	H18a，b	3.69	144.06	151.66	7.60	3.23

Compared with the existing directional medium, the invention has two advantages:

1) the MXene dispersion liquid still keeps good fluidity at the concentration of 5 wt%, and meanwhile, the MXene dispersion liquid can show narrow half-peak width in nuclear magnetic spectrum, so that higher-quality spectrogram information can be obtained conveniently.

2) The MXene nano material subjected to freeze-drying treatment still keeps the orientation capability after being redispersed, and a sample can be stored in a solid form, so that great advantages are provided for storage and transportation.

3) MXene is an inorganic material, does not contain a background signal in a two-dimensional nuclear magnetic spectrum, and avoids the interference of the background signal on the map analysis.

4) The orientation medium constructed by MXene has the behavior characteristic of lyotropic liquid crystal, and further can achieve the aim of adjusting the orientation capability of the medium by regulating and controlling the concentration, the critical concentration of the orientation capability is 0.1 wt%, and the surface of the orientation medium has more reactive active sites, so that the method provides an idea for designing and developing other novel orientation media.

In view of the above advantages, MXene constructed oriented media showed great promise in RDCs measurements. In the invention, MXene is easy to prepare by adopting a lithium fluoride and hydrochloric acid system to etch MAX phase; MXene obtained by etching can be subjected to vacuum freeze drying to obtain a solid, and the MXene has the advantage of simple storage; meanwhile, due to the redispersion characteristic, the MXene can be directly and ultrasonically redispersed to prepare the dispersion liquid for measuring the molecular RDCs, and the method is simple, has strong operability and can be used for accurate quantification. The absence of rigid aromatic groups and organic hydrocarbons in MXene avoids the risk of strong pi-pi interactions between the ordered medium and the analyte, while avoiding the effects of background signals.

Finally, the method of the present application is only a preferred embodiment and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The two-dimensional layered material MXene directional medium is characterized in that the structural formula of the two-dimensional layered material MXene is Ti_aC_bT_xT is O, F, N with hydrophilicity, and when a is 3, b is 2; when a is 2, b is 1; the oriented medium is formed by enabling the two-dimensional layered material MXene to be in H₂Forming a dispersion with a concentration of 0.1 wt% to 5 wt% in O for measurement of anisotropic nuclear magnetic parameters.

2. The MXene oriented media of two-dimensional layered material of claim 1, wherein the orientation ability of the oriented media is determined by its aspect ratio and dispersion concentration.

3. The two-dimensional layered material MXene orientation medium of claim 1, wherein the two-dimensional layered material MXene is lyophilized and then added into water, and can rapidly generate orientation ability after being ultrasonically redispersed.

4. The two-dimensional layered material MXene orientation medium of claim 1, wherein the orientation medium has a concentration of 10% D when the concentration of the two-dimensional layered material MXene is 0.1 wt% to 5 wt%₂Deuterium resonance in O solution shows residual quadrupole coupling values (RQCs) of 0-71 Hz.

5. The two-dimensional layered material MXene oriented medium of claim 1, wherein the two-dimensional layered material MXene is an inorganic layered material having no C-H coupling signal in NMR spectrum.

6. Use of the two-dimensional layered material MXene orientation medium of claim 1, which induces biomolecule orientation for molecular RDCs measurement and structure analysis.

7. The use according to claim 6, wherein the two-dimensional layered material MXene orientation medium can be used to measure RDCs of water-soluble biomolecules to obtain structural information thereof.

8. The use according to claim 7, wherein the MXene oriented mediator in a two-dimensional layered material is capable of measuring the proline structure from the RDCs of L-proline.

9. The use according to claim 7, wherein the MXene oriented media is capable of measuring sucrose RDCs to obtain sucrose structure.

10. Use according to claim 7, wherein MXene is Ti₃C₂T_x、Ti₂CT_xOr Ti₃CNT_xOne of (1) and (b).