CN114600890A - Pharmaceutical composition for preventing and treating crop nematodes, application of pharmaceutical composition and pesticide for preventing and treating crop nematodes - Google Patents
Pharmaceutical composition for preventing and treating crop nematodes, application of pharmaceutical composition and pesticide for preventing and treating crop nematodes Download PDFInfo
- Publication number
- CN114600890A CN114600890A CN202210360769.9A CN202210360769A CN114600890A CN 114600890 A CN114600890 A CN 114600890A CN 202210360769 A CN202210360769 A CN 202210360769A CN 114600890 A CN114600890 A CN 114600890A
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- China
- Prior art keywords
- preventing
- copper sulfate
- pharmaceutical composition
- nematodes
- dimethyl disulfide
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- 241000244206 Nematoda Species 0.000 title claims abstract description 44
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 27
- 239000000575 pesticide Substances 0.000 title claims abstract description 26
- WQOXQRCZOLPYPM-UHFFFAOYSA-N dimethyl disulfide Chemical compound CSSC WQOXQRCZOLPYPM-UHFFFAOYSA-N 0.000 claims abstract description 92
- 229910000365 copper sulfate Inorganic materials 0.000 claims abstract description 43
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 43
- 241000243785 Meloidogyne javanica Species 0.000 claims abstract description 14
- 241000243786 Meloidogyne incognita Species 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 22
- 239000000203 mixture Substances 0.000 abstract description 13
- 238000002360 preparation method Methods 0.000 abstract description 8
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 238000013329 compounding Methods 0.000 abstract description 3
- 230000009044 synergistic interaction Effects 0.000 abstract description 3
- 230000001094 effect on targets Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 11
- 239000003814 drug Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 239000005645 nematicide Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical group O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 239000008238 pharmaceutical water Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000482313 Globodera ellingtonae Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241001143352 Meloidogyne Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000004257 Potassium Channel Human genes 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- -1 calcium-activated potassium ion Chemical class 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004065 mitochondrial dysfunction Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 108020001213 potassium channel Proteins 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000006283 soil fumigant Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000011885 synergistic combination Substances 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticide preparations, and particularly relates to a pesticide composition for preventing and treating crop nematodes, an application of the pesticide composition and a pesticide for preventing and treating the crop nematodes. The pharmaceutical composition provided by the invention comprises dimethyl disulfide and copper sulfate. The medicinal composition is prepared by compounding the dimethyl disulfide and the copper sulfate, and the dimethyl disulfide and the copper sulfate have a synergistic interaction effect, so that the control effect on target organisms can be effectively improved, the control effect on root knot nematode can be particularly improved, and the using amount of pesticides can be reduced. The results of the examples show that the pharmaceutical composition provided by the invention has better effect on preventing and treating root knot nematode diseases, less dosage and lower use cost, and has good application in preventing and treating crop nematodes.
Description
Technical Field
The invention belongs to the technical field of pesticide preparations, and particularly relates to a pesticide composition for preventing and treating crop nematodes, an application of the pesticide composition and a pesticide for preventing and treating the crop nematodes.
Background
Plant parasitic nematodes in China are various in types, and the most significant influence on agricultural production in China is root-knot nematodes and cyst nematodes. The plant parasitic nematode has the characteristics of concealment, wide host range, easy propagation, strong pathogenicity and the like, and has the advantages of rapid population growth and great prevention and control difficulty. At present, chemical control is the most important control measure in a plurality of control measures, and dimethyl disulfide and copper sulfate have certain effects on controlling pathogenic bacteria in soil, wherein the dimethyl disulfide can be prepared into a soil fumigant for nematode control.
However, chemical nematicides suffer from the disadvantage of high consumption and low efficiency. In recent years, chemical nematicides have been banned due to problems such as resistance of nematodes and damage of chemical nematicides to the environment and non-target organisms, and therefore, development of novel and efficient nematicides is urgently required.
Disclosure of Invention
The invention aims to provide a pharmaceutical composition for preventing and treating crop nematodes and application thereof, and a pesticide for preventing and treating crop nematodes.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention provides a pharmaceutical composition for preventing and treating crop nematodes, which comprises dimethyl disulfide and copper sulfate.
Preferably, the mass ratio of the dimethyl disulfide to the copper sulfate is (2.5-80): (25-187.5).
Preferably, the mass ratio of the dimethyl disulfide to the copper sulfate is (2.5-80): 25; or the mass ratio of the dimethyl disulfide to the copper sulfate (2.5-20): 187.5.
preferably, the mass ratio of the dimethyl disulfide to the copper sulfate is (2.5-10): 25.
the invention also provides application of the pharmaceutical composition in the scheme in preparation of pesticides for preventing and treating crop nematodes.
Preferably, the crop nematodes include root knot nematodes.
Preferably, the root-knot nematode comprises melodogyne incognita.
The invention also provides a pesticide for preventing and treating crop nematodes, which comprises the pharmaceutical composition and water in the scheme.
Preferably, the mass concentration of dimethyl disulfide in the pesticide is 2.5-80 mg/L; the mass concentration of the copper sulfate is 25-187.5 mg/L.
The invention has the following beneficial effects: the invention provides a pharmaceutical composition for preventing and treating crop nematodes, which comprises dimethyl disulfide and copper sulfate. The medicinal composition is prepared by compounding the dimethyl disulfide and the copper sulfate, and the dimethyl disulfide and the copper sulfate have a synergistic interaction effect, so that the control effect on target organisms can be effectively improved, the control effect on root knot nematode can be particularly improved, and the using amount of pesticides can be reduced. The results of the examples show that the pharmaceutical composition provided by the invention has better effect on preventing and treating root knot nematode diseases, less dosage and lower use cost, and has good application in preventing and treating crop nematodes.
Detailed Description
The invention provides a pharmaceutical composition for preventing and treating crop nematodes, which comprises dimethyl disulfide and copper sulfate.
In the invention, the mass ratio of the dimethyl disulfide to the copper sulfate is preferably (2.5-80): (25-187.5), more preferably (2.5-80): 25 or (2.5-20): 187.5, most preferably (2.5-10): 25.
the sources of the dimethyl disulfide and the copper sulfate are not particularly limited, and the dimethyl disulfide and the copper sulfate can be obtained by conventional commercial methods. In the specific implementation process of the invention, the dimethyl disulfide is 98% of dimethyl disulfide raw drug, and is purchased from Shanghai Michelin Biotechnology, Inc.; the copper sulfate is copper sulfate pentahydrate, and is purchased from chemical reagents of national drug group, Inc.
In the present invention, the dimethyl dithiothreaten nematode mitochondrial dysfunction, thereby inhibiting respiratory use; and acts as a nerve agent on calcium-activated potassium ion channels of the target organism; the copper sulfate can be combined with protein in the nematode body through copper ions to generate protein salt, so that the protein is denatured and precipitated, enzyme is inactivated, and the nematode is killed finally; the synergistic interaction exists between the dimethyl disulfide and the copper sulfate, so that the prevention and control spectrum of the nematode is expanded, the use dosage and the use cost of the medicament are greatly reduced, and compared with the single use of the dimethyl disulfide and the copper sulfate, the synergistic combination can more effectively prevent and control the nematode of crops, and particularly has better effect on preventing and controlling the root-knot nematode of the crops.
The invention also provides application of the pharmaceutical composition in the scheme in preparation of pesticides for preventing and treating crop nematodes. In the present invention, the crop nematodes preferably include root knot nematodes; the root knot nematode preferably comprises Meloidogyne incognita.
The pharmaceutical composition provided by the invention can correct the mortality rate of meloidogyne incognita to 94%, can exert respective drug effects of dimethyl disulfide and copper sulfate to the maximum extent, reduce the drug consumption, reduce the drug cost and effectively reduce the meloidogyne incognita disease.
The invention also provides a pesticide for preventing and treating the crop nematodes, which comprises the pharmaceutical composition and water. In the invention, the pesticide is in the form of aqueous solution.
In the invention, the mass concentration of dimethyl disulfide in the pesticide is preferably 2.5-80 mg/L, more preferably 2.5-20 mg/L, and most preferably 2.5-10 mg/L; the mass concentration of the copper sulfate is preferably 25-187.5 mg/L, more preferably 25 or 187.5mg/L, and most preferably 25 mg/L.
In the present invention, the pesticide is preferably prepared by the following method: dissolving the dimethyl disulfide in water to obtain a dimethyl disulfide solution; dissolving the copper sulfate in water to obtain a copper sulfate solution; and mixing the dimethyl disulfide solution with a copper sulfate solution to obtain the pharmaceutical composition. In the present invention, the volume ratio of the dimethyl disulfide solution to the copper sulfate solution is preferably 1: 1.
in order to further illustrate the present invention, the following examples are given to illustrate the pharmaceutical compositions provided by the present invention and the preparation and use thereof in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
Preparation of the pharmaceutical composition:
step 1: preparation of mother liquor of dimethyl disulfide and copper sulfate
0.0820g of original dimethyl disulfide is respectively weighed by a ten-thousandth electronic balance and dissolved by water to prepare 100mL of 800 mu g/mL of mother dimethyl disulfide solution;
0.1719g of copper sulfate pentahydrate are weighed and dissolved in water to prepare 100mL of mother liquor with the copper sulfate concentration of 750 mu g/mL.
Step 2: according to the agricultural industry standard (NY/T1833.1-2009) of the people's republic of China, the control effect of the pesticide is determined by adopting an experimental insect-soaking method for inhibiting plant pathogenic nematodes.
The test nematode is Meloidogyne incognita, belonging to phylum animal, class nematoda, order of Tuliples, family Heterodermaceae and genus Meloidogyne, and is collected from the diseased root of tomato in large litterus townhe Nongravele disease field in mountain area of Beijing city, and is determined to be Meloidogyne incognita through morphological and molecular identification.
Washing the egg mass of root-knot nematode from living host plant with water, placing on wet filter paper of culture dish, and incubating at 25 deg.C to obtain second-instar larva with consistent age.
The dimethyl disulfide and copper sulfate are respectively diluted into each concentration gradient solution. Different concentrations of dimethyl disulfide were mixed with different copper sulfate solutions 1: 1 and mixing. 0.5mL of nematode suspension with a concentration of 100 heads/mL is added into a 2mL centrifuge tube, then 0.5mL of mixed solution of the test reagent is added, so that the final concentration of dimethyl disulfide in the test reagent forms two concentrations of 25mg/L and 187.5mg/L, the final concentration of copper sulfate in the test reagent forms two concentrations of dimethyl disulfide in the test reagent, the dimethyl disulfide in the series of concentration gradients is mixed with two concentrations of copper sulfate and a blank containing no medicament treatment, each treatment is repeated three times, and the average value is taken. Culturing at 25 deg.C for 24 h.
And 4, step 4: and calculating and analyzing results. 0.5mL of mixed solution is taken from each process, the death condition of the nematodes is observed under a dissecting mirror, and the number of the bus worms and the number of dead line worms are recorded. Dead nematodes are stiff and still cannot move in a bending manner when touched by a hair pin.
Mortality and corrected mortality in percent (%) were calculated for each treatment based on survey data.
And (4) calculating according to the formulas (1) and (2), and reserving the calculation results to the last two decimal points.
In the formula: p1Mortality rate; k, number of dead nematode; n investigates the number of bus worms.
In the formula: p2Correcting for mortality; ptTreating the mortality rate of the nematodes; p0Control nematode mortality.
Using statistical software to make regression analysis according to the logarithmic value of the concentration of the drug and the number value of the corrected mortality of the nematodes, and calculating LC of the drug50And its 95% confidence limit.
The corresponding theoretical prevention effect is calculated according to a Colby method, and then compared with the actual prevention effect of the mixed agent, so that the effect of the mixed agent is conveniently and effectively evaluated. When the theory of binary mixture is used as the control effect E0<When the actual control effect is E, the binary mixture is synergistic; when the theory of binary mixture is used as the control effect E0>When the actual control effect is E, the binary mixture is antagonistic. Calculating according to the formula (3):
in the formula, E0The theory control effect of the binary mixture is achieved; x1Is the control effect of the 1 st medicament; x2Is the control effect of the 2 nd medicament.
TABLE 1 indoor toxicity test results of the compounded preparation of the present invention against root-knot nematodes
As can be seen from the records in Table 1, the indoor activity measurement result shows that the pharmaceutical composition prepared by compounding dimethyl disulfide and copper sulfate has a synergistic effect on Meloidogyne incognita. The corrected mortality rate of the medicinal composition compounded by 12 kinds of dimethyl disulfide and copper sulfate with different dosages on the meloidogyne incognita reaches more than 74.70 percent, wherein the medicinal composition compounded by a dimethyl disulfide solution with the concentration of 2.5mg/L and a copper sulfate solution with the concentration of 25mg/L ensures that the actual prevention effect of the meloidogyne incognita is 94.59 percent. The results show that the dimethyl disulfide and the copper sulfate in the pharmaceutical composition have a synergistic effect when used for preventing and treating the meloidogyne incognita, and have a remarkable prevention and treatment effect on the meloidogyne incognita, the cost is reduced, and the pollution to the soil environment can be remarkably reduced under the condition of using a lower concentration.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and all of the embodiments are included in the scope of the present invention.
Claims (9)
1. A pharmaceutical composition for preventing and treating crop nematodes is characterized by comprising dimethyl disulfide and copper sulfate.
2. The pharmaceutical composition according to claim 1, wherein the mass ratio of the dimethyl disulfide to the copper sulfate is (2.5-80): (25-187.5).
3. The pharmaceutical composition according to claim 2, wherein the mass ratio of the dimethyl disulfide to the copper sulfate is (2.5-80): 25; or the mass ratio of the dimethyl disulfide to the copper sulfate is (2.5-20): 187.5.
4. the pharmaceutical composition according to claim 3, wherein the mass ratio of the dimethyl disulfide to the copper sulfate is (2.5-10): 25.
5. use of the pharmaceutical composition of any one of claims 1 to 4 in pesticides for controlling nematodes in crops.
6. The use of claim 5, wherein the crop nematodes include root knot nematodes.
7. The use of claim 6, wherein the root knot nematode comprises Meloidogyne incognita.
8. A pesticide for controlling crop nematodes, comprising the pharmaceutical composition of any one of claims 1 to 4 and water.
9. The pesticide of claim 8, wherein the mass concentration of dimethyl disulfide in the pesticide is 2.5-80 mg/L; the mass concentration of the copper sulfate is 25-187.5 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210360769.9A CN114600890A (en) | 2022-04-07 | 2022-04-07 | Pharmaceutical composition for preventing and treating crop nematodes, application of pharmaceutical composition and pesticide for preventing and treating crop nematodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210360769.9A CN114600890A (en) | 2022-04-07 | 2022-04-07 | Pharmaceutical composition for preventing and treating crop nematodes, application of pharmaceutical composition and pesticide for preventing and treating crop nematodes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114600890A true CN114600890A (en) | 2022-06-10 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210360769.9A Pending CN114600890A (en) | 2022-04-07 | 2022-04-07 | Pharmaceutical composition for preventing and treating crop nematodes, application of pharmaceutical composition and pesticide for preventing and treating crop nematodes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114600890A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491242A (en) * | 2008-01-24 | 2009-07-29 | 中国农业科学院植物保护研究所 | Application technique of dimethyl disulfide as soil fumigant |
| US20110244056A1 (en) * | 2010-03-30 | 2011-10-06 | University Of Central Florida Research Foundation, Inc. | Multifunctional silica-based compositions and gels, methods of making them, and methods of using them |
-
2022
- 2022-04-07 CN CN202210360769.9A patent/CN114600890A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491242A (en) * | 2008-01-24 | 2009-07-29 | 中国农业科学院植物保护研究所 | Application technique of dimethyl disulfide as soil fumigant |
| US20110244056A1 (en) * | 2010-03-30 | 2011-10-06 | University Of Central Florida Research Foundation, Inc. | Multifunctional silica-based compositions and gels, methods of making them, and methods of using them |
Non-Patent Citations (1)
| Title |
|---|
| 白春明 等: "无机化合物对南方根结线虫作用方式的研究", 《植物保护》 * |
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Application publication date: 20220610 |