CN114594040A - Flow cytometer and working method thereof - Google Patents
Flow cytometer and working method thereof Download PDFInfo
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- CN114594040A CN114594040A CN202210289518.6A CN202210289518A CN114594040A CN 114594040 A CN114594040 A CN 114594040A CN 202210289518 A CN202210289518 A CN 202210289518A CN 114594040 A CN114594040 A CN 114594040A
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 207
- 238000001514 detection method Methods 0.000 claims abstract description 52
- 230000003287 optical effect Effects 0.000 claims abstract description 43
- 239000012530 fluid Substances 0.000 claims description 67
- 238000004140 cleaning Methods 0.000 claims description 53
- 239000002699 waste material Substances 0.000 claims description 24
- 238000005070 sampling Methods 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 12
- 238000004891 communication Methods 0.000 claims description 8
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 230000002572 peristaltic effect Effects 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 101
- 239000002245 particle Substances 0.000 description 11
- 238000006073 displacement reaction Methods 0.000 description 9
- 239000006285 cell suspension Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 2
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- 238000004364 calculation method Methods 0.000 description 1
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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Abstract
A flow cytometer and a working method thereof relate to the technical field of medical detection, and comprise the following steps: the system comprises a host, a liquid storage system and an analysis system, wherein after a sample is introduced, the sample is analyzed through an optical system, meanwhile, the flow of the sample output into a sample output pipe by a liquid conveying pipe and the flow of sheath liquid output to a flow chamber by a sheath liquid pump are respectively obtained through a first flow meter and a second flow meter, the operation speeds of a quantitative pump and the sheath liquid pump are adjusted through analyzing the information obtained by the optical system, the detection speed of the flow cytometer is maximized under the condition that the detection precision is kept, and the problem that the detection precision cannot be kept due to the increase of the detection speed in the current flow cytometer is solved.
Description
Technical Field
The invention relates to the technical field of medical detection, in particular to a flow cytometer and a working method thereof.
Background
The flow cytometer may be used to analyze a sample fluid having samples or particles, and identify characteristics of the samples or particles contained in the fluid, where the samples or particles may be biological samples or physical samples collected for analysis and/or separation, the samples or particles may be mixed with a sheath fluid to be transported by the flow cytometer, and the particles may include biological samples, calibration beads, physical sample particles or other particles of interest, and specific information of the samples may be obtained by analyzing the particles through an optical system, where the optical system is composed of a laser, an optical lens, an optical fiber, a filter, a photodetector, etc., and a laser irradiated by the laser to irradiate a laser on the photodetector when the laser irradiates particulate matters in the sample at an outlet of a flow cell to obtain a sensing signal, and the information of the samples may be obtained by analyzing the sensing signal, the speed of the detected sample flow can be controlled by controlling the speed of the plunger of the quantitative pump for feeding and pushing the sample, so that various detection speeds from low speed to high speed are formed, and the problem exists at present that the lower the detection speed is, the smaller the diameter of the sample flow is, and the more central the particles are arranged in the flow chamber; since the higher the detection speed, the larger the sample flow diameter, the lower the precision of the signal intensity of the detection result due to the deterioration of the degree of alignment of the particles in the flow cell, the lower the detection speed if the higher the detection precision is sought, and the higher the detection speed is desired, the higher the detection speed is, the detection speed and the detection precision cannot be compatible.
Disclosure of Invention
The embodiment of the invention provides a flow cytometer and a working method thereof, wherein after a sample is injected, the sample is analyzed through an optical system, meanwhile, the flow of the sample output from a liquid conveying pipe to a sample output pipe and the flow of sheath liquid output from a sheath liquid pump to a flow chamber are respectively obtained through a first flow meter and a second flow meter, the information obtained through the optical system is analyzed, and the running speeds of the quantitative pump and the sheath liquid pump are adjusted, so that the detection speed of the flow cytometer is maximized under the condition of keeping the detection precision, and the problem that the detection precision cannot be kept due to the increase of the detection speed in the conventional flow cytometer is solved.
A flow cytometer includes, in combination,
the host comprises a sample introduction system, a sheath fluid conveying system, a flow chamber, an optical system and a controller;
the sample introduction system and the sheath fluid conveying system are respectively communicated with the flow chamber, the optical system is arranged at an outlet of the flow chamber and is used for detecting a sample at the outlet of the flow chamber, and the controller is respectively in communication connection with the sample introduction system and the sheath fluid conveying system;
the liquid storage system comprises a liquid storage platform, a sheath liquid barrel, a waste liquid barrel and a cleaning liquid bottle;
the sheath liquid barrel, the cleaning liquid bottle and the waste liquid barrel are sequentially arranged on the liquid storage platform, the sheath liquid barrel is communicated with the sheath liquid conveying system through a pipeline, the waste liquid barrel is communicated with an outlet of the flowing chamber through a pipeline, and the cleaning liquid bottle is communicated with the sample injection system through a pipeline;
the analysis system is applied to the flow cytometer and comprises a signal acquisition module, a signal analysis module and an adjustment module;
the signal acquisition module is used for acquiring data of the sample introduction system and the sheath fluid conveying system and also used for acquiring detection data acquired by the optical system;
the signal analysis module is used for analyzing the data acquired by the signal acquisition module to obtain an analysis result;
and the adjusting module is used for adjusting the sample introduction system and the sheath fluid conveying system according to the analysis result obtained by the signal analysis module.
Further, the sampling system comprises a dosing pump, a liquid storage pipeline, a three-way joint, a sampling needle tube, a valve, a sample output tube and a first flowmeter, wherein the sampling needle tube is communicated with a first interface of the valve through a pipeline, a second interface of the valve is communicated with the first interface of the three-way joint through a pipeline, a third interface of the valve is communicated with the cleaning liquid bottle through a pipeline, the dosing pump is communicated with a second interface of the three-way joint through the liquid storage pipeline, the third interface of the three-way joint is communicated with the input end of the sample output tube through a pipeline, the output end of the sample output tube is communicated with the input end of the flow chamber, the first flowmeter is arranged between the liquid storage pipeline and the input end of the sample output tube and is used for acquiring the flow of a sample output by a liquid transfer tube in the sample output tube, and a check valve is arranged at the outlet of the third interface of the three-way adapter.
Further, the sheath fluid delivery system includes a sheath fluid pump and a second flow meter, an input end of the sheath fluid pump communicates with the sheath fluid through a pipeline, an output end of the sheath fluid pump communicates with an input end of the flow chamber through a pipeline, the second flow meter is disposed between the sheath fluid pump and the flow chamber and is used for obtaining a flow rate of the sheath fluid output to the flow chamber by the sheath fluid pump, and the sheath fluid output to the flow chamber wraps the sample output pipe and outputs the sample to the flow chamber.
Further, the sheath fluid pump is a peristaltic pump, and the quantitative pump is a syringe pump.
Further, the input end of the controller is respectively connected with the first flowmeter, the second flowmeter and the optical system in a communication manner, and the output end of the controller is respectively connected with the signal input ends of the quantitative pump, the sheath fluid pump and the valve in a communication manner.
Furthermore, the signal acquisition module comprises a first acquisition unit, a second acquisition unit and a third acquisition unit, the first acquisition unit is used for acquiring data of the first flowmeter, the second acquisition unit is used for acquiring data of the second flowmeter, and the third acquisition unit is used for acquiring data of the optical system.
Further, the adjusting module is used for adjusting the output flow of the sheath liquid pump and the dosing pump.
In a second aspect, an embodiment of the present invention provides a method for operating a flow cytometer, including the following steps:
s1, cleaning for the first time, opening a third interface of a valve, a second interface of the valve and a constant delivery pump through a controller, sucking cleaning liquid in a cleaning liquid bottle into a liquid storage pipeline through the third interface of the valve, the second interface of the valve, a first interface of a three-way adapter and a second interface of the three-way adapter by the constant delivery pump in sequence, closing the valve, outputting the cleaning liquid sucked in the liquid storage pipeline to a sample output pipe through the third interface of the three-way adapter by the constant delivery pump to enter a flow chamber, synchronously inputting sheath liquid in a sheath liquid barrel into the flow chamber by a sheath liquid pump, allowing the cleaning liquid in the flow chamber to enter a waste liquid barrel through a pipeline, disconnecting the constant delivery pump from the cleaning liquid bottle, and emptying the cleaning liquid in the flow chamber through the sheath liquid pump and the constant delivery pump;
s2, sample introduction, inserting the sampling needle tube into the sample, connecting the sheath liquid pump with the sheath liquid barrel, opening the sheath liquid pump, the valve and the quantitative pump through the controller, firstly, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, the sheath liquid in the flow chamber enters the waste liquid barrel through the pipeline, the second flowmeter obtains the flow of the sheath liquid entering the flow chamber, secondly, the quantitative pump sucks the sample into the liquid storage pipeline through the sampling needle tube and the first interface of the valve, the second interface of the valve, the first interface of the three-way adapter and the second interface of the three-way adapter in turn, the valve is closed, the quantitative pump outputs a sample sucked in the liquid storage pipeline to the sample output pipe through a third interface of the three-way adapter and enters the flow chamber, the sheath liquid drives the sample to pass through an outlet of the flow chamber, the optical system obtains information of the sample, and the sheath liquid and the sample are input into the waste liquid barrel through the pipeline;
s3, carrying out system analysis, wherein the first collector, the second collector and the third collector respectively collect data of the first flowmeter, the second flowmeter and the optical system, and the signal analysis module obtains an analysis result through analysis of the data of the optical system;
s4, adjusting, wherein the adjusting module respectively adjusts the quantitative pump and the sheath fluid pump according to the analysis result and the data of the first flowmeter and the second flowmeter acquired by the first collector and the second collector, and maximizes the detection speed under the condition of keeping the detection precision;
s5, cleaning for the second time, after sample detection is completed, opening a third interface of a valve, a second interface of the valve and a constant delivery pump through a controller, sucking cleaning liquid in a cleaning liquid bottle into a liquid storage pipeline through the third interface of the valve, the second interface of the valve, the first interface of a three-way adapter and the second interface of the three-way adapter by the constant delivery pump, closing the valve, outputting the cleaning liquid sucked in the liquid storage pipeline to a sample output pipe through the third interface of the three-way adapter by the constant delivery pump, entering a flow chamber through a sheath liquid pump, inputting sheath liquid in a sheath liquid barrel into the flow chamber, entering the cleaning liquid in the flow chamber into a waste liquid barrel through a pipeline, disconnecting the constant delivery pump from the cleaning liquid bottle, and emptying the cleaning liquid in the flow chamber through the sheath liquid pump and the constant delivery pump.
The technical scheme provided by the embodiment of the invention has the beneficial effects that at least:
after a sample is injected, the sample is analyzed through an optical system, meanwhile, the flow of the sample output to a sample output pipe by a liquid conveying pipe and the flow of sheath liquid output to a flow chamber by a sheath liquid pump are respectively obtained through the first flow meter and the second flow meter, the operation speeds of the quantitative pump and the sheath liquid pump are adjusted through analyzing information obtained by the optical system, the detection speed of the flow cytometer is maximized under the condition that the detection precision is kept, and the problem that the detection precision cannot be kept due to the fact that the detection speed is increased in the existing flow cytometer is solved.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural diagram of a flow cytometer according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of an analysis system according to an embodiment of the present invention;
fig. 3 is a schematic flow chart of a flow cytometer according to an embodiment of the present invention.
Reference numerals:
1. a host; 11. a sample introduction system; 111. a constant delivery pump; 112. a liquid storage pipeline; 113. a three-way adapter; 114. a sampling needle tube; 115. a valve; 116. a sample output pipe; 117. a first flow meter; 12. a sheath fluid delivery system; 121. a sheath fluid pump; 122. a second flow meter; 13. a flow chamber; 14. an optical system; 15. a controller; 16. a cell suspension concentration sensor; 2. a liquid storage system; 21. a liquid storage platform; 22. a sheath liquid barrel; 23. a waste liquid barrel; 24. cleaning solution bottles; 3. an analysis system; 31. a signal acquisition module; 311. a first collector; 312. a second collector; 313. a third collector; 32. a signal analysis module; 33. an adjustment module; 34. a sample concentration obtaining module; 35. and a control parameter presetting module.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
Referring to fig. 1-2, an embodiment of the present invention provides a flow cytometer, which includes a host 1, a liquid storage system 2, and an analysis system 3, after a sample is injected by the host 1, the sample is analyzed by an optical system 14 inside the host 1, and simultaneously, a flow rate of the sample output from a liquid delivery tube to a sample output tube 116 and a flow rate of sheath liquid output from a sheath liquid pump 121 to a flow chamber 13 are respectively obtained by a first flow meter 117 and a second flow meter 122, and the analysis system 3 analyzes information obtained by the optical system 14 and adjusts operating speeds of the quantitative pump 111 and the sheath liquid pump 121, so that a detection speed of the flow cytometer is maximized while a detection accuracy is maintained, and a problem that the detection accuracy cannot be maintained due to an increase in the detection speed of the current flow cytometer is solved.
Referring to fig. 1-2, in the embodiment of the present invention, the host 1 includes a sample injection system 11, a sheath fluid delivery system 12, a flow chamber 13, an optical system 14, and a controller 15, the host 1 further includes a cell suspension concentration sensor 16 disposed between a sample needle 114 and a valve 115 for obtaining a concentration of a sample, the sample injection system 11 and the sheath fluid delivery system 12 are respectively communicated with the flow chamber 13, the optical system 14 is disposed at an outlet of the flow chamber 13 for detecting the sample at the outlet of the flow chamber 13, the controller 15 is respectively communicated with the sample injection system 11 and the sheath fluid delivery system 12, the sample injection system 11 includes a constant delivery pump 111, a fluid storage pipeline 112, a three-way adapter 113, a sample needle 114, a valve 115, a sample output pipe 116, and a first flow meter 117, the sample needle 114 is communicated with an input port of the valve 115 through a pipeline, an output port of the valve 115 is communicated with a first interface of the three-way adapter 113 through a pipeline, the dosing pump 111 is communicated with a second interface of the three-way joint 113 through the liquid storage pipeline 112, a third interface of the three-way joint 113 is communicated with an input end of the sample output pipe 116 through a pipeline, an output end of the sample output pipe 116 is communicated with an input end of the flow chamber 13, the first flow meter 117 is arranged between the liquid storage pipeline 112 and the input end of the sample output pipe 116 and is used for acquiring the flow rate of the sample output by the liquid conveying pipe into the sample output pipe 116, a check valve is arranged at the third interface of the three-way joint 113 and is used for preventing the sample and the sheath fluid in the flow chamber 13 from flowing back, the sheath fluid conveying system 12 comprises a sheath fluid pump 121 and a second flow meter 122, the input end of the sheath fluid pump 121 is communicated with the sheath fluid through a pipeline, the output end of the sheath fluid pump 121 is communicated with the input end of the flow chamber 13 through a pipeline, the second flow meter 122 is arranged between the sheath fluid pump 121 and the flow chamber 13 and is used for acquiring the flow rate of the sheath fluid output from the sheath fluid pump 121 to the flow chamber 13, the sheath liquid pump 121 outputs sheath liquid of the sheath liquid to the flow chamber 13 to wrap the sample output pipe 116 and output the sample to the flow chamber 13, the sheath liquid pump 121 is a peristaltic pump, the constant delivery pump 111 is an injection pump, the input end of the controller 15 is respectively in communication connection with the first flow meter 117, the second flow meter 122 and the optical system 14, the output end of the controller 15 is respectively in communication connection with the constant delivery pump 111, the sheath liquid pump 121 and the signal input end of the valve 115, the liquid storage system 2 comprises a liquid storage platform 21, a sheath liquid barrel 22, a waste liquid barrel 23 and a cleaning liquid bottle 24, the sheath liquid barrel 22, the cleaning liquid bottle 24 and the waste liquid barrel 23 are sequentially arranged on the liquid storage platform 21, the sheath liquid barrel 22 is communicated with the sheath liquid delivery system 12 through a pipeline, the waste liquid barrel 23 is communicated with the outlet of the flow chamber 13 through a pipeline, the cleaning liquid bottle 24 is communicated with the sampling system 11 through a pipeline, a needle tube 114 is inserted into the sample, the sheath liquid pump 121 is communicated with the sheath liquid barrel 22, the sheath liquid pump 121, the valve 115 and the quantitative pump 111 are opened by the controller 15, firstly, the sheath liquid pump 121 inputs the sheath liquid in the sheath liquid barrel 22 into the flow chamber 13, the sheath liquid in the flow chamber 13 enters the waste liquid barrel 23 through a pipeline, the second flow meter 122 obtains the flow rate of the sheath liquid entering the flow chamber 13, secondly, the quantitative pump 111 sucks the sample into the liquid storage pipeline 112 through the sampling needle tube 114 by sequentially passing through the first interface of the valve 115, the second interface of the valve 115, the first interface of the three-way adapter 113 and the second interface of the three-way adapter 113, the valve 115 is closed, the quantitative pump 111 outputs the sample sucked into the liquid storage pipeline 112 to the sample output pipe 116 to enter the flow chamber 13 through the third interface of the three-way adapter 113, the sheath liquid drives the sample to pass through the outlet of the flow chamber 13, the optical system 14 obtains the information of the sample, the sheath liquid and the sample are input into the waste liquid barrel 23 through a pipeline, and the analysis system 3 analyzes the information obtained by the optical system 14, the operation speeds of the quantitative pump 111 and the sheath fluid pump 121 are adjusted to maximize the detection speed of the flow cytometer under the condition of keeping the detection precision, so that the problem that the detection precision cannot be kept by increasing the detection speed of the current flow cytometer is solved.
Referring to fig. 1-2, in the embodiment of the present invention, the analysis system 3 is disposed on the controller 15 and applied to a flow cytometer, and includes a signal acquisition module 31, a signal analysis module 32, and an adjustment module 33;
the signal acquisition module 31 is configured to acquire data of the sample introduction system 11 and the sheath fluid delivery system 12, and is further configured to acquire detection data acquired by the optical system 14.
Specifically, the signal acquiring module 31 includes a first acquirer 311, a second acquirer 312, and a third acquirer 313, where the first acquirer 311 is configured to acquire data of the first flow meter 117, the second acquirer 312 is configured to acquire data of the second flow meter 122, and the third acquirer 313 is configured to acquire data of the optical system 14.
The signal analysis module 32 is configured to analyze the data acquired by the signal acquisition module 31 to obtain an analysis result, the adjustment module 33 is configured to adjust the sample injection system 11 and the sheath fluid delivery system 12 according to the analysis result obtained by the signal analysis module 32, and the adjustment module 33 is configured to adjust the output flow rates of the sheath fluid pump 121 and the fixed displacement pump 111;
specifically, the signal analysis module 32 obtains data of the first flowmeter 117 and the second flowmeter 122, and analyzes the data of the optical system 14, specifically, the analysis process is as follows: when the quantitative pump 111 outputs a sample and the sheath liquid pump 121 outputs sheath liquid, the quantitative pump operates at full speed, at this time, the flow rates obtained by the first collector 311 and the second collector 312 are maximum, the signal analysis module 32 analyzes the waveform of the optical detector signal in the optical system 14, when the waveform is unstable, the adjustment module 33 adjusts the output flow rates of the sheath liquid pump 121 and the quantitative pump 111 respectively, so that the output flow rate of the sheath liquid pump 121 is greater than the output flow rate of the quantitative pump 111, the increase of the diameter of the sample flow caused by accumulation when the sample is output from the sample output pipe 116 to the flow chamber 13 is avoided, the waveform is unstable, after the waveform is stable, the adjustment module 33 continues to adjust the output flow rates of the sheath liquid pump 121 and the quantitative pump 111 respectively, the waveform obtained by the signal analysis module 32 is stable while the output flow rate of the sheath liquid pump 121 is ensured to be greater than the output flow rate of the quantitative pump 111, and the distance between wave peaks reaches the minimum, at this time, the output flow rates of the fixed displacement pump 111 and the sheath fluid pump 121 ensure that the diameter of the sample flow is minimum, and the particles are arranged most centrally in the flow chamber 13, so that the effect of maintaining the detection accuracy while the detection speed reaches the maximum value is achieved.
The analysis system 3 further comprises a sample concentration obtaining module 34 and a control parameter presetting module 35, the sample concentration obtaining module 34 collects concentration data of a sample obtained by the cell suspension concentration sensor 16, the control parameter presetting module 35 obtains concentration of the current sample and parameters of the sheath liquid pump 121 and the quantitative pump 111 currently adjusted by the adjusting module 33 and stores the parameters, when a sample with the same concentration is detected, the stored parameters are output to the adjusting module 33, the adjusting module 33 adjusts output flow rates of the quantitative pump 111 and the sheath liquid pump 121 according to the received parameters to perform rapid adjustment, the adjusting module 33 adjusts according to an analysis result of the signal analysis module 32, the analysis result is that a waveform is stable and when an interval between peaks reaches a minimum, the adjusting module 33 does not adjust, and when other analysis results occur, the adjusting module 33 continues to respectively adjust the output flow rates of the sheath liquid pump 121 and the quantitative pump 111, when the output flow of the sheath fluid pump 121 is larger than the output flow of the fixed displacement pump 111, the waveform obtained by the signal analysis module 32 is stable, the distance between wave crests is minimum, and meanwhile, the adjustment parameter of the adjustment module 33 is updated to the control parameter presetting module 35, so that the problem that the detection precision cannot be maintained due to the fact that the detection speed is increased in the conventional flow cytometer is solved.
Referring to fig. 1-3, the present invention further provides a method for operating a flow cytometer, comprising the steps of:
s1, cleaning for the first time, opening the third interface of the valve 115, the second interface of the valve 115, and the fixed displacement pump 111 through the controller 15, the fixed displacement pump 111 sucking the cleaning liquid in the cleaning liquid bottle 24 into the liquid storage pipe 112 through the third interface of the valve 115, the second interface of the valve 115, the first interface of the three-way joint 113, and the second interface of the three-way joint 113, closing the valve 115, the fixed displacement pump 111 outputting the cleaning liquid sucked in the liquid storage pipe 112 to the sample output pipe 116 through the third interface of the three-way joint 113 and entering the flow chamber 13, and synchronously, the sheath liquid pump 121 inputs the sheath liquid in the sheath liquid barrel 22 into the flow chamber 13, the cleaning liquid in the flow chamber 13 enters the waste liquid barrel 23 through the pipeline, the fixed displacement pump 111 is disconnected from the bottle 24, and the cleaning liquid in the sheath liquid pump 121 and the fixed displacement pump 111 is emptied;
s2, introducing a sample, inserting a sampling needle tube 114 into the sample, communicating a sheath liquid pump 121 with a sheath liquid barrel 22, opening a sheath liquid pump 121, a valve 115 and a dosing pump 111 through a controller 15, firstly, the sheath liquid pump 121 inputs the sheath liquid in the sheath liquid barrel 22 into a flow chamber 13, the sheath liquid pump 121 operates at full speed and outputs maximum flow, the sheath liquid in the flow chamber 13 enters a waste liquid barrel 23 through a pipeline, a second flow meter 122 acquires the flow of the sheath liquid entering the flow chamber 13, secondly, the dosing pump 111 sucks the sample into a liquid storage pipeline 112 through the sampling needle tube 114 and sequentially through the valve 115, a first interface of a three-way adapter 113 and a second interface of the three-way adapter 113, closing the valve 115, the dosing pump 111 operates at full speed and outputs maximum flow, the dosing pump 111 outputs the sample sucked in the liquid storage pipeline 112 to a sample output tube 116 through a third interface of the three-way adapter 113 and enters the flow chamber 13, the sheath fluid drives the sample to pass through an outlet of the flow chamber 13, the optical system 14 obtains information of the sample, and the sheath fluid and the sample are input into the waste liquid barrel 23 through a pipeline;
s3, performing system analysis, in which the first collector 311, the second collector 312, and the third collector 313 collect data of the first flowmeter 117, the second flowmeter 122, and the optical system 14, respectively, and the signal analysis module 32 obtains an analysis result by analyzing the data of the optical system 14;
s4, adjusting, the adjusting module 33 adjusts the quantitative pump 111 and the sheath fluid pump 121 according to the analysis result and the data of the first flowmeter 117 and the second flowmeter 122 acquired by the first acquirer 311 and the second acquirer 312, respectively, so as to maximize the detection speed while maintaining the detection accuracy;
s5, performing the second cleaning, after the sample detection is completed, opening the third interface of the valve 115, the second interface of the valve 115, and the dosing pump 111 through the controller 15, sucking the cleaning solution in the cleaning solution bottle 24 into the solution storage pipeline 112 through the third interface of the valve 115, the second interface of the valve 115, the first interface of the three-way adapter 113, and the second interface of the three-way adapter 113 by the dosing pump 111, closing the valve 115, outputting the cleaning solution sucked in the solution storage pipeline 112 to the sample output pipe 116 through the third interface of the three-way adapter 113 by the dosing pump 111, and entering the flow chamber 13 through the sheath pump 121, inputting the sheath solution in the sheath barrel 22 into the flow chamber 13 by the sheath pump 121, entering the cleaning solution in the flow chamber 13 into the waste barrel 23 through the pipeline, disconnecting the dosing pump 111 from the cleaning solution bottle 24, and evacuating the cleaning solution inside through the sheath pump 121 and the dosing pump 111.
Specifically, after the sample is injected, the sample is analyzed through the optical system 14, and meanwhile, the flow rate of the sample output from the infusion tube to the sample output tube 116 and the flow rate of the sheath fluid output from the sheath fluid pump 121 to the flow chamber 13 are respectively obtained through the first flow meter 117 and the second flow meter 122, and the information obtained through the optical system 14 is analyzed, so that the operation speeds of the quantitative pump 111 and the sheath fluid pump 121 are adjusted, the detection speed of the flow cytometer is maximized under the condition of maintaining the detection precision, and the problem that the detection precision cannot be maintained due to the increase of the detection speed in the current flow cytometer is solved.
It should be noted that the specific model specifications of the fixed displacement pump 111, the valve 115, the first flow meter 117, the sheath fluid pump 121, the second flow meter 122, and the controller 15 need to be determined by type selection according to the actual specification of the device, and the specific type selection calculation method adopts the prior art in the field, and therefore, detailed description is omitted.
The power supply and the principle of the dosing pump 111, the valve 115, the first flow meter 117, the sheath fluid pump 121, the second flow meter 122 and the controller 15 will be clear to those skilled in the art and will not be described in detail herein.
It should be understood that the specific order or hierarchy of steps in the processes disclosed is an example of exemplary approaches. Based upon design preferences, it is understood that the specific order or hierarchy of steps in the processes may be rearranged without departing from the scope of the present disclosure. The accompanying method claims present elements of the various steps in a sample order, and are not intended to be limited to the specific order or hierarchy presented.
In the foregoing detailed description, various features are grouped together in a single embodiment for the purpose of streamlining the disclosure. This method of disclosure is not to be interpreted as reflecting an intention that the claimed embodiments of the subject matter require more features than are expressly recited in each claim. Rather, as the following claims reflect, invention lies in less than all features of a single disclosed embodiment. Thus, the following claims are hereby expressly incorporated into the detailed description, with each claim standing on its own as a separate preferred embodiment of the invention.
Those of skill would further appreciate that the various illustrative logical blocks, modules, circuits, and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware, computer software, or combinations of both. To clearly illustrate this interchangeability of hardware and software, various illustrative components, blocks, modules, circuits, and steps have been described above generally in terms of their functionality. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the overall system. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present disclosure.
The steps of a method or algorithm described in connection with the embodiments disclosed herein may be embodied directly in hardware, in a software module executed by a processor, or in a combination of the two. A software module may reside in RAM memory, flash memory, ROM memory, EPROM memory, EEPROM memory, registers, hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to the processor such the processor can read information from, and write information to, the storage medium. Of course, the storage medium may also be integral to the processor. The processor and the storage medium may reside in an ASIC. The ASIC may reside in a user terminal. Of course, the processor and the storage medium may reside as discrete components in a user terminal.
For a software implementation, the techniques described herein may be implemented with modules (e.g., procedures, functions, and so on) that perform the functions described herein. The software codes may be stored in memory units and executed by processors. The memory unit may be implemented within the processor or external to the processor, in which case it can be communicatively coupled to the processor via various means as is known in the art.
What has been described above includes examples of one or more embodiments. It is, of course, not possible to describe every conceivable combination of components or methodologies for purposes of describing the aforementioned embodiments, but one of ordinary skill in the art may recognize that many further combinations and permutations of various embodiments are possible. Accordingly, the embodiments described herein are intended to embrace all such alterations, modifications and variations that fall within the scope of the appended claims. Furthermore, to the extent that the term "includes" is used in either the detailed description or the claims, such term is intended to be inclusive in a manner similar to the term "comprising" as "comprising" is interpreted when employed as a transitional word in a claim. Furthermore, any use of the term "or" in the specification of the claims is intended to mean a "non-exclusive or".
Claims (8)
1. A flow cytometer, comprising,
the host comprises a sample introduction system, a sheath fluid conveying system, a flow chamber, an optical system and a controller;
the sample introduction system and the sheath fluid conveying system are respectively communicated with the flow chamber, the optical system is arranged at an outlet of the flow chamber and is used for detecting a sample at the outlet of the flow chamber, and the controller is respectively in communication connection with the sample introduction system and the sheath fluid conveying system;
the liquid storage system comprises a liquid storage platform, a sheath liquid barrel, a waste liquid barrel and a cleaning liquid bottle;
the sheath liquid barrel, the cleaning liquid bottle and the waste liquid barrel are sequentially arranged on the liquid storage platform, the sheath liquid barrel is communicated with the sheath liquid conveying system through a pipeline, the waste liquid barrel is communicated with an outlet of the flowing chamber through a pipeline, and the cleaning liquid bottle is communicated with the sample injection system through a pipeline;
the analysis system is applied to the flow cytometer and comprises a signal acquisition module, a signal analysis module and an adjustment module;
the signal acquisition module is used for acquiring data of the sample introduction system and the sheath fluid conveying system and also used for acquiring detection data acquired by the optical system;
the signal analysis module is used for analyzing the data acquired by the signal acquisition module to obtain an analysis result;
and the adjusting module is used for adjusting the sample introduction system and the sheath fluid conveying system according to the analysis result obtained by the signal analysis module.
2. The flow cytometer of claim 1, wherein the sample introduction system comprises a quantitative pump, a liquid storage pipeline, a three-way joint, a sampling needle tube, a valve, a sample output pipe and a first flowmeter, wherein the sampling needle tube is communicated with the first interface of the valve through a pipeline, the second interface of the valve is communicated with the first interface of the three-way joint through a pipeline, the third interface of the valve is communicated with the cleaning solution bottle through a pipeline, the quantitative pump is communicated with the second interface of the three-way joint through the liquid storage pipeline, the third interface of the three-way joint is communicated with the input end of the sample output pipe through a pipeline, the output end of the sample output pipe is communicated with the input end of the flow chamber, and the first flowmeter is disposed between the liquid storage pipeline and the input end of the sample output pipe, the three-way adapter is used for acquiring the flow of the sample output by the infusion tube into the sample output tube, and a check valve is arranged at the third interface outlet of the three-way adapter.
3. The flow cytometer of claim 2 wherein the sheath fluid delivery system comprises a sheath fluid pump and a second flow meter, wherein an input end of the sheath fluid pump is connected to the sheath fluid channel via a pipe, an output end of the sheath fluid pump is connected to an input end of the flow chamber via a pipe, the second flow meter is disposed between the sheath fluid pump and the flow chamber for obtaining a flow rate of the sheath fluid output from the sheath fluid pump to the flow chamber, and the sheath fluid output from the sheath fluid pump to the flow chamber wraps the sample output from the sample output tube to the flow chamber.
4. A flow cytometer as described in claim 3 wherein said sheath fluid pump is a peristaltic pump and said metering pump is a syringe pump.
5. The flow cytometer of claim 4 wherein the controller has inputs in communication with the first flow meter, the second flow meter, and the optical system, respectively, and an output in communication with the signal inputs of the dosing pump, the sheath fluid pump, and the valve, respectively.
6. The flow cytometer of claim 3 wherein the signal collection module comprises a first collector, a second collector and a third collector, wherein the first collector is used for collecting data of the first flow meter, the second collector is used for collecting data of the second flow meter, and the third collector is used for collecting data of the optical system.
7. The flow cytometer of claim 3 wherein the adjustment module is configured to adjust the output flow rates of the sheath pump and the dosing pump.
8. A method of operating a flow cytometer as described in claims 1-7, comprising the steps of:
s1, cleaning for the first time, opening a third interface of a valve, a second interface of the valve and a constant delivery pump through a controller, sucking cleaning liquid in a cleaning liquid bottle into a liquid storage pipeline through the third interface of the valve, the second interface of the valve, a first interface of a three-way adapter and a second interface of the three-way adapter by the constant delivery pump in sequence, closing the valve, outputting the cleaning liquid sucked in the liquid storage pipeline to a sample output pipe through the third interface of the three-way adapter by the constant delivery pump to enter a flow chamber, synchronously inputting sheath liquid in a sheath liquid barrel into the flow chamber by a sheath liquid pump, allowing the cleaning liquid in the flow chamber to enter a waste liquid barrel through a pipeline, disconnecting the constant delivery pump from the cleaning liquid bottle, and emptying the cleaning liquid in the flow chamber through the sheath liquid pump and the constant delivery pump;
s2, sample introduction, inserting the sampling needle tube into the sample, connecting the sheath liquid pump with the sheath liquid barrel, opening the sheath liquid pump, the valve and the quantitative pump through the controller, firstly, the sheath liquid pump inputs the sheath liquid in the sheath liquid barrel into the flow chamber, the sheath liquid in the flow chamber enters the waste liquid barrel through the pipeline, the second flowmeter obtains the flow of the sheath liquid entering the flow chamber, secondly, the quantitative pump sucks the sample into the liquid storage pipeline through the sampling needle tube and the first interface of the valve, the second interface of the valve, the first interface of the three-way adapter and the second interface of the three-way adapter in turn, the valve is closed, the quantitative pump outputs a sample sucked in the liquid storage pipeline to the sample output pipe through a third interface of the three-way adapter and enters the flow chamber, the sheath liquid drives the sample to pass through an outlet of the flow chamber, the optical system obtains information of the sample, and the sheath liquid and the sample are input into the waste liquid barrel through the pipeline;
s3, carrying out system analysis, wherein the first collector, the second collector and the third collector respectively collect data of the first flowmeter, the second flowmeter and the optical system, and the signal analysis module obtains an analysis result through analysis of the data of the optical system;
s4, adjusting, wherein the adjusting module respectively adjusts the quantitative pump and the sheath fluid pump according to the analysis result and the data of the first flowmeter and the second flowmeter acquired by the first acquisition device and the second acquisition device, and maximizes the detection speed under the condition of keeping the detection precision;
s5, secondary cleaning, after sample detection is finished, opening a third interface of a valve, a second interface of the valve and a constant delivery pump through a controller, sucking cleaning liquid in a cleaning liquid bottle into a liquid storage pipeline through the third interface of the valve, a second interface of the valve, a first interface of a three-way joint and a second interface of the three-way joint by the constant delivery pump, closing the valve, outputting the cleaning liquid sucked in the liquid storage pipeline to a sample output pipe through the third interface of the three-way joint by the constant delivery pump, entering a flow chamber, synchronously inputting sheath liquid in a sheath liquid barrel into the flow chamber by a sheath liquid pump, entering the cleaning liquid in the flow chamber into a waste liquid barrel through a pipeline, disconnecting the constant delivery pump from the cleaning liquid bottle, and evacuating the internal cleaning liquid through the sheath liquid pump and the constant delivery pump.
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| CN202210289518.6A CN114594040A (en) | 2022-03-23 | 2022-03-23 | Flow cytometer and working method thereof |
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| CN202210289518.6A CN114594040A (en) | 2022-03-23 | 2022-03-23 | Flow cytometer and working method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078324A (en) * | 2022-06-30 | 2022-09-20 | 嘉兴市唯真生物科技有限公司 | High-flux flow type fluorescence detection method, intelligent terminal and storage medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115078324A (en) * | 2022-06-30 | 2022-09-20 | 嘉兴市唯真生物科技有限公司 | High-flux flow type fluorescence detection method, intelligent terminal and storage medium |
| CN115078324B (en) * | 2022-06-30 | 2023-03-10 | 嘉兴市唯真生物科技有限公司 | High-throughput flow-type fluorescence detection method, intelligent terminal and storage medium |
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