CN114592277A - Strontium-doped silicon dioxide nanofiber membrane and preparation method thereof - Google Patents
Strontium-doped silicon dioxide nanofiber membrane and preparation method thereof Download PDFInfo
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- CN114592277A CN114592277A CN202210225483.XA CN202210225483A CN114592277A CN 114592277 A CN114592277 A CN 114592277A CN 202210225483 A CN202210225483 A CN 202210225483A CN 114592277 A CN114592277 A CN 114592277A
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 title claims abstract description 122
- 239000002121 nanofiber Substances 0.000 title claims abstract description 80
- 239000012528 membrane Substances 0.000 title claims abstract description 70
- 239000000377 silicon dioxide Substances 0.000 title claims abstract description 68
- 235000012239 silicon dioxide Nutrition 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000000243 solution Substances 0.000 claims abstract description 52
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 27
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 27
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 26
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000007864 aqueous solution Substances 0.000 claims abstract description 20
- DHEQXMRUPNDRPG-UHFFFAOYSA-N strontium nitrate Chemical compound [Sr+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O DHEQXMRUPNDRPG-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000001354 calcination Methods 0.000 claims abstract description 14
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 11
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 claims abstract description 10
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- 229910052712 strontium Inorganic materials 0.000 abstract description 14
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- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 abstract description 12
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 3
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- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
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- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4209—Inorganic fibres
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F6/00—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof
- D01F6/44—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polymers obtained by reactions only involving carbon-to-carbon unsaturated bonds as major constituent with other polymers or low-molecular-weight compounds
- D01F6/50—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polymers obtained by reactions only involving carbon-to-carbon unsaturated bonds as major constituent with other polymers or low-molecular-weight compounds of polyalcohols, polyacetals or polyketals
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4282—Addition polymers
- D04H1/4309—Polyvinyl alcohol
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
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Abstract
The invention provides a strontium-doped silicon dioxide nanofiber membrane and a preparation method thereof, wherein the preparation method comprises the following steps: mixing ethyl orthosilicate, deionized water and phosphoric acid to obtain SiO2Sol solution; to SiO2Adding strontium nitrate into the sol solution to obtain a mixed sol solution; preparing a polyvinyl alcohol aqueous solution; mixing a polyvinyl alcohol aqueous solution with the mixed sol solution to obtain an electrostatic spinning solution; carrying out electrostatic spinning on the electrostatic spinning solution to obtain a nanofiber membrane; and calcining the nanofiber membrane to obtain the strontium-doped silicon dioxide nanofiber membrane. The invention providesThe preparation method of the strontium-doped silicon dioxide nanofiber membrane has the advantages of simple preparation process and wide raw material source, the prepared strontium-doped silicon dioxide nanofiber membrane has good biocompatibility, and meanwhile, the ion release speed is slow, so that the strontium is prevented from being suddenly released, the strontium-doped silicon dioxide nanofiber membrane can keep the bioactivity for a long time, the regeneration of bone tissues is promoted, and the bone repair effect is improved.
Description
Technical Field
The invention relates to the technical field of inorganic nano fibers, in particular to a strontium-doped silicon dioxide nano fiber film and a preparation method thereof.
Background
Vascularized autologous bone grafting is the current gold standard for the treatment of large bone defects caused by trauma, infection, tumor or ischemic osteonecrosis. However, clinical use of autologous bone grafts has been limited due to limited sources, risk of infection and immune rejection. Therefore, in clinical bone defect repair, vascularized bone tissue engineering is urgently required as an important substitute for implant materials.
Angiogenesis is one of the prerequisites for new bone formation and remodeling in the bone repair process. The formation of new blood vessels is essential for the exchange of oxygen, nutrients and metabolic waste products during bone regeneration. Improved angiogenesis has a positive effect on osteoblast maturation, calcification and bone remodeling. However, most of the research on calcium phosphate-based materials has focused on their physical and chemical properties, and neglects the role of angiogenesis in the bone regeneration process. Calcium phosphate lacks the ability to promote vascularization and is therefore ineffective in vascularized bone regeneration, limiting its use in tissue engineering. Thus, certain bioactive substances such as Vascular Endothelial Growth Factor (VEGF) and bioactive metal ions such as strontium (Sr), magnesium, iron and copper ions have been incorporated into calcium phosphate to improve angiogenesis. However, growth factors are easily degraded and lose activity in vivo due to short half-life and lack of suitable carriers in conventionally used drugs; of all bioactive ions, Sr is at the forefront of research due to its ability to promote osteoblasts and inhibit osteoclasts. In recent years, various Sr-or SrR-composed materials, including bioceramic scaffolds, cements, and bioactive glasses, have been developed for bone regeneration; however, most of strontium prepared by the traditional preparation method is doped into the material in a particle form, and burst release is easy to occur, so that the aim of expecting angiogenesis promotion cannot be well achieved, and the bone repair effect is influenced.
Disclosure of Invention
The invention solves the problem that the bone repair effect is influenced because strontium is easy to produce burst release in the existing bone repair material.
In order to solve the problems, the invention provides a preparation method of a strontium-doped silicon dioxide nanofiber membrane, which comprises the following steps:
s1: mixing ethyl orthosilicate, deionized water and phosphoric acid to obtain SiO2Sol solution;
s2: to the SiO2Adding strontium nitrate into the sol solution, and stirring at room temperature to obtain a mixed sol solution;
s3: preparing a polyvinyl alcohol aqueous solution;
s4: mixing the polyvinyl alcohol aqueous solution with the mixed sol solution to obtain an electrostatic spinning solution;
s5: carrying out electrostatic spinning on the electrostatic spinning solution to obtain a nanofiber membrane;
s6: and calcining the nanofiber membrane to obtain the strontium-doped silicon dioxide nanofiber membrane.
Optionally, the molar ratio of the ethyl orthosilicate, the deionized water and the phosphoric acid in the step S1 is 1:11: 0.01.
Optionally, the reaction time of step S1 ranges from 20h to 40 h.
Optionally, the strontium nitrate and the SiO in step S22The mass ratio range of the sol solution is (0.001-0.003): 1.
alternatively, the mass concentration of the polyvinyl alcohol aqueous solution in step S3 is 10%.
Optionally, the mass ratio of the polyvinyl alcohol aqueous solution to the mixed sol solution in step S4 is 1: 1.
Optionally, the parameters of electrospinning the electrospinning solution in step S5 are: the voltage is 20kV, the advancing speed is 2ml/h, the receiving device is an aluminum foil, and the receiving distance is 20 cm.
Alternatively, the calcination temperature in step S6 ranges from 700 ℃ to 900 ℃.
Alternatively, the calcination time in step S6 ranges from 1h to 3 h.
Another object of the present invention is to provide a strontium-doped silica nanofiber membrane prepared by the method for preparing the strontium-doped silica nanofiber membrane as described above.
Compared with the prior art, the preparation method of the strontium-doped silicon dioxide nanofiber membrane provided by the invention has the following advantages:
the preparation method of the strontium-doped silicon dioxide nanofiber membrane provided by the invention has the advantages that the preparation process is simple, the raw material source is wide, the prepared strontium-doped silicon dioxide nanofiber membrane has good biocompatibility, the ion release speed is slow, the strontium is prevented from being suddenly released, and the strontium-doped silicon dioxide nanofiber membrane has the characteristic of slow degradation, so that the bioactivity can be maintained for a long time, the regeneration of bone tissues is promoted, and the bone repair effect is improved.
Drawings
FIG. 1 is a scanning electron microscope image of a nanofiber membrane and a strontium-doped silica nanofiber membrane prepared in example 1 of the present invention;
FIG. 2 is the proliferation of NIH-3T3 cells on strontium doped silica nanofiber membranes in example 1 of the present invention;
FIG. 3 is the electron microscope image of NIH-3T3 cultured on strontium-doped silica nanofiber membrane for 7 days in the example of the present invention.
Detailed Description
The following describes embodiments of the present invention in detail. The embodiments described below with reference to the drawings are exemplary and intended to be illustrative of the present invention and should not be construed as limiting the present invention, and all other embodiments that can be obtained by one skilled in the art based on the embodiments of the present invention without inventive efforts shall fall within the scope of protection of the present invention.
In order to solve the problem that the bone repair effect is affected because strontium is easy to produce burst release in the existing bone repair material, the invention provides a strontium-doped silicon dioxide nanofiber membrane and a preparation method thereof, wherein the preparation method comprises the following steps:
s1: mixing ethyl orthosilicate, deionized water and phosphoric acid to obtain SiO2Sol solution;
s2: to SiO2Adding strontium nitrate into the sol solution, and stirring at room temperature to obtain a mixed sol solution;
s3: preparing a polyvinyl alcohol aqueous solution;
s4: mixing a polyvinyl alcohol aqueous solution with the mixed sol solution to obtain an electrostatic spinning solution;
s5: carrying out electrostatic spinning on the electrostatic spinning solution to obtain a nanofiber membrane;
s6: and calcining the nanofiber membrane to obtain the strontium-doped silicon dioxide nanofiber membrane.
According to the preparation method of the strontium-doped silicon dioxide nanofiber membrane, strontium is introduced into electrostatic spinning solution by taking polyvinyl alcohol as a template agent, the nanofiber membrane is prepared through electrostatic spinning, and the template agent is removed through calcination to obtain the strontium-doped silicon dioxide nanofiber membrane with bioactivity; this strontium doping silica nanofiber membrane promotes angiogenesis through introducing Sr, and through preparing this inorganic active ingredient strontium into inorganic nanofiber structure, this inorganic nanofiber structure has good flexibility on the one hand, can bionical extracellular matrix through this flexible inorganic nanofiber structure, the inorganic component of bone has better simulation, good biocompatibility has, on the other hand this flexible inorganic nanofiber structure ion release speed is slow, avoid strontium to take place the burst release, make this strontium doping silica nanofiber membrane have the characteristic of slow degradation, thereby can keep the biological activity for a long time, promote bone tissue regeneration, improve bone repair effect.
The preparation method of the strontium-doped silicon dioxide nanofiber membrane provided by the invention has the advantages that the preparation process is simple, the raw material source is wide, the prepared strontium-doped silicon dioxide nanofiber membrane has good biocompatibility, the ion release speed is slow, the strontium is prevented from being suddenly released, and the strontium-doped silicon dioxide nanofiber membrane has the characteristic of slow degradation, so that the bioactivity can be maintained for a long time, the regeneration of bone tissues is promoted, and the bone repair effect is improved.
In order to ensure the bone repair effect of the strontium-doped silicon dioxide nanofiber membrane, the molar ratio of the tetraethoxysilane to the deionized water to the phosphoric acid in the step S1 is preferably 1:11: 0.01; the reaction time range of the step S1 is 20-40 h; preferably, strontium nitrate and SiO are used in step S22The mass ratio range of the sol solution is (0.001-0.003): 1; in the step S3, the mass concentration of the polyvinyl alcohol aqueous solution is 10%; in the step S4, the mass ratio of the polyvinyl alcohol aqueous solution to the mixed sol solution is 1: 1; in step S5, the parameters of electrospinning the electrospinning solution are: the voltage is 20kV, the advancing speed is 2ml/h, the receiving device is an aluminum foil, and the receiving distance is 20 cm; the calcining temperature range in the step S6 is 700-900 ℃; the calcination time in step S6 ranges from 1h to 3 h.
Another object of the present invention is to provide a strontium-doped silica nanofiber membrane prepared by the method for preparing the strontium-doped silica nanofiber membrane as described above.
The strontium-doped silicon dioxide nanofiber membrane provided by the invention has good flexibility, and the flexible inorganic nanofiber structure can simulate extracellular matrix, better simulate inorganic components of bones and have good biocompatibility; the flexible inorganic nanofiber structure has slow release speed of ions, avoids strontium burst release, and enables the strontium-doped silicon dioxide nanofiber membrane to have the characteristic of slow degradation, so that the biological activity can be maintained for a long time, the regeneration of bone tissues is promoted, and the bone repair effect is improved.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
Example 1
S1: ethyl orthosilicate, deionized water and phosphoric acid are mixed according to a molar ratio of 1:11:0.01, stirring for 30h to obtain SiO2Sol solution;
s2: to 10g of SiO2Adding 10mg of strontium nitrate into the sol solution, and stirring at room temperature to obtain a mixed sol solution;
s3: dissolving 2g of polyvinyl alcohol (PVA) in 18g of water, and stirring for 12 hours to obtain a 10% polyvinyl alcohol aqueous solution;
s4: mixing the polyvinyl alcohol aqueous solution and the mixed sol solution in equal mass to obtain an electrostatic spinning solution;
s5: adding the electrostatic spinning solution into an injector, mounting the injector on a propulsion pump, connecting high voltage, setting the electrostatic spinning high voltage to be 20kV, the propulsion speed to be 2ml/h, using an aluminum foil as a receiving device, and carrying out electrostatic spinning to prepare a nanofiber membrane, wherein the receiving distance is 20 cm;
s6: and (3) placing the nanofiber membrane in a muffle furnace, calcining for 2h at 800 ℃, and removing the template agent to obtain the strontium-doped silicon dioxide nanofiber membrane.
Electron microscope scanning is performed on the nanofiber membrane prepared in the step S5 and the strontium-doped silica nanofiber membrane prepared in the step S6, as shown in fig. 1, the strontium-doped silica nanofiber membrane prepared in the embodiment has a nanofiber structure, can simulate an extracellular matrix, has good biocompatibility, and is beneficial to promoting the generation of blood.
In order to facilitate the detection of the performance of the prepared strontium-doped silica nanofiber membrane, the embodiment further performs bacterial treatment and cell culture on the prepared strontium-doped silica nanofiber membrane, and specifically includes the following steps:
s7: cutting the strontium-doped silicon dioxide nanofiber membrane into a wafer with the diameter of 14mm, then placing the wafer-shaped strontium-doped silicon dioxide nanofiber membrane to be used for cell planting into a 24-hole cell culture plate, sterilizing for 60min under irradiation of an ultraviolet lamp, and sterilizing for 30min with 70% ethanol; washing with PBS for three times after sterilization;
s8: the suspension concentration of the cells was adjusted to 10X 10 by means of a Countstar cell automatic counter after NIH-3T3 was removed from the cell culture flask4Adding 500 mu L of suspension into each hole, then quickly transferring the suspension into a cell constant-temperature incubator for culture, and culturing cells in a conventional mode;
s9: when the cells were cultured for 1, 4, and 7 days, the medium in the well plate was aspirated, followed by washing off the residual medium with PBS, then 400 μ L of incomplete medium containing 10% CCK-8 was added to each well with material, incubation for 90min in the absence of light was performed, then 100 μ L was taken from each well and transferred to a 96 well plate, and absorbance was measured at a wavelength of 450nm with a microplate reader for each well.
Referring to fig. 2 and 3, the strontium-doped silica nanofiber membrane provided by the embodiment has an inorganic nanofiber structure which is beneficial to cell adhesion and proliferation and osteoblast gene expression, and is expected to be used in the fields of clinical medicine and bone tissue engineering.
Example 2
S1: ethyl orthosilicate, deionized water and phosphoric acid are mixed according to a molar ratio of 1:11:0.01, stirring for 40 hours to obtain SiO2Sol solution;
s2: to 10g of SiO2Adding 20mg of strontium nitrate into the sol solution, and stirring at room temperature to obtain a mixed sol solution;
s3: dissolving 2g of polyvinyl alcohol (PVA) in 18g of water, and stirring for 12 hours to obtain a 10% polyvinyl alcohol aqueous solution;
s4: mixing the polyvinyl alcohol aqueous solution and the mixed sol solution in equal mass to obtain an electrostatic spinning solution;
s5: adding the electrostatic spinning solution into an injector, mounting the injector on a propulsion pump, connecting high voltage, setting the electrostatic spinning high voltage to be 20kV, the propulsion speed to be 2ml/h, using an aluminum foil as a receiving device, and carrying out electrostatic spinning to prepare a nanofiber membrane, wherein the receiving distance is 20 cm;
s6: and (3) placing the nanofiber membrane in a muffle furnace, calcining for 1h at 900 ℃, and removing the template agent to obtain the strontium-doped silicon dioxide nanofiber membrane.
See example 1 for relevant information on the detection process.
Example 3
S1: ethyl orthosilicate, deionized water and phosphoric acid are mixed according to a molar ratio of 1:11:0.01, stirring for 20 hours to obtain SiO2Sol solution;
s2: to 10g of SiO2Adding 30mg of strontium nitrate into the sol solution, and stirring at room temperature to obtain a mixed sol solution;
s3: dissolving 2g of polyvinyl alcohol (PVA) in 18g of water, and stirring for 12 hours to obtain a 10% polyvinyl alcohol aqueous solution;
s4: mixing the polyvinyl alcohol aqueous solution and the mixed sol solution in equal mass to obtain an electrostatic spinning solution;
s5: adding the electrostatic spinning solution into an injector, mounting the injector on a propulsion pump, connecting high voltage, setting the electrostatic spinning high voltage to be 20kV, the propulsion speed to be 2ml/h, taking an aluminum foil as a receiving device, and carrying out electrostatic spinning to prepare a nanofiber membrane, wherein the receiving distance is 20 cm;
s6: and (3) placing the nanofiber membrane in a muffle furnace, calcining for 3h at 700 ℃, and removing the template agent to obtain the strontium-doped silicon dioxide nanofiber membrane.
See example 1 for relevant information on the detection process.
Although the present disclosure has been described above, the scope of the present disclosure is not limited thereto. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present disclosure, and such changes and modifications will fall within the scope of the present invention.
Claims (10)
1. A preparation method of a strontium-doped silicon dioxide nanofiber membrane is characterized by comprising the following steps:
s1: mixing ethyl orthosilicate, deionized water and phosphoric acid to obtain SiO2Sol solution;
s2: to the SiO2Adding strontium nitrate into the sol solution, and stirring at room temperature to obtain a mixed sol solution;
s3: preparing a polyvinyl alcohol aqueous solution;
s4: mixing the polyvinyl alcohol aqueous solution with the mixed sol solution to obtain an electrostatic spinning solution;
s5: carrying out electrostatic spinning on the electrostatic spinning solution to obtain a nanofiber membrane;
s6: and calcining the nanofiber membrane to obtain the strontium-doped silicon dioxide nanofiber membrane.
2. The method of claim 1, wherein a molar ratio of the tetraethoxysilane, the deionized water and the phosphoric acid in step S1 is 1:11: 0.01.
3. The method for preparing a strontium-doped silica nanofiber membrane according to claim 1, wherein the reaction time of step S1 is in the range of 20h to 40 h.
4. The method of preparing a strontium-doped silica nanofiber membrane according to claim 1, wherein the strontium nitrate and the SiO are mixed in step S22The mass ratio range of the sol solution is (0.001-0.003): 1.
5. the method for preparing a strontium-doped silica nanofiber membrane according to claim 1, wherein the mass concentration of the polyvinyl alcohol aqueous solution in step S3 is 10%.
6. The method for preparing the strontium-doped silica nanofiber membrane according to claim 1, wherein the mass ratio of the polyvinyl alcohol aqueous solution to the mixed sol solution in step S4 is 1: 1.
7. The method for preparing the strontium-doped silica nanofiber membrane according to claim 1, wherein the parameters of electrospinning the electrospinning solution in step S5 are as follows: the voltage is 20kV, the advancing speed is 2ml/h, the receiving device is an aluminum foil, and the receiving distance is 20 cm.
8. The method for preparing a strontium-doped silica nanofiber membrane according to claim 1, wherein the calcination temperature in step S6 is in the range of 700 ℃ to 900 ℃.
9. The method for preparing a strontium-doped silica nanofiber membrane according to claim 1, wherein the calcination time in step S6 is in the range of 1h to 3 h.
10. A strontium-doped silica nanofiber membrane prepared by the method for preparing a strontium-doped silica nanofiber membrane according to any one of claims 1 to 9.
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