CN114568599A - Premix mate biological function bag for improving intestinal health of laying hens and application thereof - Google Patents
Premix mate biological function bag for improving intestinal health of laying hens and application thereof Download PDFInfo
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- CN114568599A CN114568599A CN202210004048.4A CN202210004048A CN114568599A CN 114568599 A CN114568599 A CN 114568599A CN 202210004048 A CN202210004048 A CN 202210004048A CN 114568599 A CN114568599 A CN 114568599A
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- 230000007413 intestinal health Effects 0.000 title claims abstract description 29
- 230000008827 biological function Effects 0.000 title claims abstract description 22
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 22
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 239000004310 lactic acid Substances 0.000 claims abstract description 11
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 11
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 10
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 10
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 10
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 10
- 108091005804 Peptidases Proteins 0.000 claims abstract description 10
- 239000004365 Protease Substances 0.000 claims abstract description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 10
- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 10
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 10
- 231100000678 Mycotoxin Toxicity 0.000 claims abstract description 9
- 239000002636 mycotoxin Substances 0.000 claims abstract description 9
- 239000005711 Benzoic acid Substances 0.000 claims abstract description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 8
- 235000010233 benzoic acid Nutrition 0.000 claims abstract description 8
- 239000000341 volatile oil Substances 0.000 claims abstract description 8
- 239000003463 adsorbent Substances 0.000 claims abstract description 6
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical group O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 229910000278 bentonite Inorganic materials 0.000 claims description 10
- 239000000440 bentonite Substances 0.000 claims description 10
- 238000001354 calcination Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 150000002303 glucose derivatives Chemical class 0.000 claims description 3
- 230000008595 infiltration Effects 0.000 claims description 3
- 238000001764 infiltration Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 235000019621 digestibility Nutrition 0.000 abstract description 6
- 239000011248 coating agent Substances 0.000 abstract description 4
- 238000000576 coating method Methods 0.000 abstract description 4
- 230000036750 egg laying performance Effects 0.000 abstract description 3
- 230000017448 oviposition Effects 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 102000002322 Egg Proteins Human genes 0.000 abstract description 2
- 108010000912 Egg Proteins Proteins 0.000 abstract description 2
- -1 beta-mannase Proteins 0.000 abstract description 2
- 210000003278 egg shell Anatomy 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 15
- 238000012360 testing method Methods 0.000 description 9
- 235000013601 eggs Nutrition 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 230000002354 daily effect Effects 0.000 description 6
- 239000006041 probiotic Substances 0.000 description 6
- 235000018291 probiotics Nutrition 0.000 description 6
- 239000000654 additive Substances 0.000 description 5
- 230000003203 everyday effect Effects 0.000 description 5
- 238000004806 packaging method and process Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 208000004232 Enteritis Diseases 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 235000014590 basal diet Nutrition 0.000 description 3
- 235000013330 chicken meat Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229930195730 Aflatoxin Natural products 0.000 description 2
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000005409 aflatoxin Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000433 anti-nutritional effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000006027 corn-soybean meal Substances 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108700037728 Glycine max beta-conglycinin Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 108010083391 glycinin Proteins 0.000 description 1
- BCQZXOMGPXTTIC-UHFFFAOYSA-N halothane Chemical compound FC(F)(F)C(Cl)Br BCQZXOMGPXTTIC-UHFFFAOYSA-N 0.000 description 1
- 108010046721 halothane dehalogenase Proteins 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000003170 nutritional factors Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to the technical field of feed preparation, and particularly relates to a premix mate biological function bag for improving intestinal health of laying hens and application thereof. The functional bag comprises the following raw materials: glucose oxidase, beta-mannase, protease, bacillus subtilis, clostridium butyricum, coating lactic acid bacteria, mycotoxin adsorbent, benzoic acid, plant essential oil and a saccharomyces cerevisiae culture. The biological function bag provided by the invention is matched with different grades of premix in the market for use, so that the intestinal health of laying hens is protected from multiple angles, the excrement is prevented from being too thin, and the excrement forming degree is improved; the digestibility of the laying hens on the feed is improved, and the phenomenon of feed passing is effectively reduced; the laying hens are guaranteed to exert high-efficiency egg laying performance, and the egg laying peak period is prolonged; improve the quality and color of the eggshell and reduce the egg breaking rate.
Description
Technical Field
The invention belongs to the technical field of feed preparation, and particularly relates to a premix mate biological function bag for improving intestinal health of laying hens and application thereof.
Background
At present, the laying hens are cultivated for longer and longer periods, which generally exceed 500 days, and even are cultivated for more than 600 days under the condition of good market conditions. The laying peak period of the laying hens is maintained for longer and longer time, and the excellent breeding farm can maintain the laying rate of more than 90% for more than 10 months. Under the market conditions that the price of eggs is continuously lower, the profit of laying hen breeding is lower and lower, and the prolongation of the egg laying peak period becomes the basis of the profit of laying hen breeding. The important basis for ensuring the laying hens to exert the high-efficiency production capacity is intestinal health. However, poor intestinal health is still a common problem in the breeding end at present, which is manifested by thin and unformed feces, yellow feces and undigested feed raw material particles commonly called 'overfeeding'. As the feed is completely forbidden to be added with antibiotics and is strongly regulated by the drug residues in eggs by government departments, intestinal diseases such as enteritis and the like cannot be treated by using the antibiotics at the breeding end.
Some premix manufacturers add green additive products such as enzyme preparations, probiotics and the like into the premix to improve the intestinal health state of the laying hens and the digestibility of the feed. However, due to the limitation of the formula cost, the probiotics are mainly bacillus, and high-quality additives such as lactic acid bacteria and the like with higher price are difficult to add into the premix. In addition, the premix mainly contains major elements such as calcium, phosphorus, sodium and the like, trace elements such as iron, zinc, copper, manganese, selenium, iodine and the like, vitamins and part of restrictive amino acids, and is a basic nutrient substance which must be taken by the laying hens every day. For laying hens with good intestinal health state, the cost is wasted when the premix containing various additives for protecting the intestinal tract is fed every day, and for probiotics with intestinal colonization ability, the laying hens are not required to be fed every day. Some farms can also purchase one or more probiotics such as bacillus, lactobacillus and clostridium butyricum to be added into the feed by themselves. However, there are many nutritional factors affecting the intestinal health of laying hens, including antigen substances which are easy to cause enteritis, such as beta-mannan, glycinin, beta-conglycinin and trypsin inhibitor in the soybean meal raw material, mycotoxin in corn and bran, and pathogenic microorganisms, such as escherichia coli, etc., and it is difficult to solve the intestinal health problem well only by means of a probiotic single additive product.
Disclosure of Invention
The invention mainly aims to provide a premix mate biological function bag for improving the intestinal health of laying hens, aiming at the problem of the intestinal health of the laying hens.
The invention also provides a preparation method of the premix mate biological function bag.
The invention also aims to provide application of the premix mate biological function bag.
The technical scheme adopted by the invention for realizing the purpose is as follows:
the invention provides a premix mate biological function bag for improving intestinal health of laying hens, which comprises the following raw materials in parts by weight: 1-5 parts of glucose oxidase, 1-5 parts of beta-mannase, 0.5-2 parts of protease, 0.1-1 part of bacillus subtilis, 0.1-1 part of clostridium butyricum, 1-5 parts of coated lactic acid bacteria, 5-15 parts of mycotoxin adsorbent, 10-50 parts of benzoic acid, 1-10 parts of plant essential oil and 30-70 parts of saccharomyces cerevisiae culture.
Further, in the raw materials: glucose oxidase is 1000 IU/g, beta-mannase is 50000 IU/g, protease is 50000 IU/g, bacillus subtilis is 1000 hundred million/g, clostridium butyricum is 100 hundred million/g, and coated lactic acid bacteria is 100 hundred million/g.
The mycotoxin adsorbent used in the invention is modified bentonite.
The modified bentonite is prepared by the following method:
(1) adding acetic acid solution into bentonite, stirring for 30-40min, filtering, and adding saturated glucose solution into the filtered bentonite for infiltration;
(2) soaking for 1h, filtering, and calcining the soaked bentonite.
Further, the mass concentration of the acetic acid solution is 16-18%.
Further, the calcination is calcination at 400-450 ℃ for 1.5 h.
The invention also provides application of the premix mate biological function bag in improving the intestinal health of the laying hens, and when the premix mate biological function bag is fed in a pulse type feeding mode, the feeding is stopped for 1 day and 2 days, and the feeding is stopped for 1 day and 3 days.
The Saccharomyces cerevisiae culture used in the present invention was Neoplene, purchased from Atlzer bioproduct, Inc.
The premix mate biological function bag provided by the invention is prepared by uniformly mixing the raw materials by a mixer, packaging the uniformly mixed raw materials into function bags by 5 kg of packaging bags, and adding one bag of the function bag into each ton of full-price daily ration of laying hens.
Through the combination and proportion of the additive raw materials, the intestinal health of the laying hens is synergistically protected from different angles. The glucose oxidase consumes oxygen in the intestinal tract by catalyzing the reaction of glucose and oxygen, so that the growth and the propagation of aerobic harmful bacteria such as escherichia coli, salmonella and the like in the intestinal tract are inhibited, and simultaneously, the hydrogen peroxide generated by the reaction can play a role in sterilization; the plant essential oil can inhibit and kill harmful bacteria in intestinal tract; the bacillus subtilis and the lactic acid bacteria supplement probiotics for the intestinal tract and compete with harmful bacteria for nutrient substances and intestinal tract colonization sites; the beta-mannase and the protease degrade anti-nutritional factors in the feed raw materials, and reduce enteritis reaction caused by the anti-nutritional factors; the mycotoxin degrading agent degrades mycotoxin in the feed raw material; benzoic acid regulates the pH value of the intestinal tract and plays a certain bacteriostatic role.
In the second aspect, the feed can be flexibly used according to the intestinal health condition of the laying hens, the mildew degree of feed raw materials, different growth stages of the laying hens and the grade of the used premix. The compound premix for the laying hens with poor intestinal health state is used for a period of time, the compound premix can be stopped after the intestinal health is improved, the compound premix can be continuously used, and a pulse type feeding mode can also be adopted to carry out health care on the intestinal tracts of the laying hens. The pulse feeding mode comprises feeding for 1 day and stopping for 1 day, feeding for 1 day and stopping for 2 days, and feeding for 1 day and stopping for 3 days. Different pulse type feeding modes can be selected for health care of the intestinal tracts of laying hens in a normal intestinal health state, laying hens aged for 350 days, common premix, fresh corn, high raw material mildew rate and the like.
The invention has the beneficial effects that:
the biological function bag provided by the invention is matched with different grades of premix in the market for use, so that the intestinal health of laying hens is protected from multiple angles, the excrement is prevented from being too thin, and the excrement forming degree is improved; the digestibility of the laying hens on the feed is improved, and the phenomenon of feed passing is effectively reduced; the laying hens are guaranteed to exert high-efficiency egg laying performance, and the egg laying peak period is prolonged; improve the quality and color of the eggshell and reduce the egg breaking rate. In the use method, the premix can be used for the health condition of the intestinal tract of the laying hens every day or at intervals by adopting a pulse type adding mode, so that the intestinal tract health problem caused by different nutritional reasons can be solved through a green technology, the eggs are ensured to have no antibiotic residue, the premix can be flexibly used independently according to the actual needs in the breeding process, and the cost is saved. The compound feed is mutually supplemented with premix in a companion form, so that the intestinal health state of the laying hens is improved, the digestibility of the feed is improved, and the egg laying performance of the laying hens is further improved.
Detailed Description
The technical solution of the present invention is further explained and illustrated by the following specific examples.
Example 1
The formula is as follows: 4 parts of glucose oxidase, 2 parts of beta-mannase, 1 part of protease, 0.4 part of bacillus subtilis, 0.4 part of clostridium butyricum, 2 parts of coating lactic acid bacteria, 10 parts of modified bentonite, 30 parts of benzoic acid, 6 parts of vegetable essential oil and 44.2 parts of a saccharomyces cerevisiae culture.
The modified bentonite is prepared by the following method:
(1) adding 18% acetic acid solution into bentonite, stirring for 40min, filtering, and adding saturated glucose solution into the filtered bentonite for infiltration;
(2) soaking for 1h, filtering, and calcining the soaked bentonite for 1.5h at the temperature of 400-450 ℃.
And after being uniformly mixed by a mixer, the mixture is subpackaged into functional bags by adopting packaging bags with the specification of 5 kg.
Example 2
The formula is as follows: 5 parts of glucose oxidase, 1 part of beta-mannase, 1 part of protease, 0.8 part of bacillus subtilis, 0.1 part of clostridium butyricum, 3 parts of coating lactic acid bacteria, 10 parts of modified bentonite, 15 parts of benzoic acid, 3 parts of vegetable essential oil and 55 parts of a saccharomyces cerevisiae culture.
The modified bentonite was prepared in the same manner as in example 1.
After being uniformly mixed by a mixer, the mixture is subpackaged into functional bags by adopting packaging bags with the specification of 5 kilograms.
Example 3
The formula is as follows: 3 parts of glucose oxidase, 3 parts of beta-mannase, 2 parts of protease, 0.3 part of bacillus subtilis, 1 part of clostridium butyricum, 3 parts of coating lactic acid bacteria, 10 parts of modified bentonite, 10-18 parts of benzoic acid, 6 parts of plant essential oil and 45 parts of a saccharomyces cerevisiae culture.
The modified bentonite was prepared in the same manner as in example 1.
After being uniformly mixed by a mixer, the mixture is subpackaged into functional bags by adopting packaging bags with the specification of 5 kilograms.
Example 4
The formula is as follows: 4 parts of glucose oxidase, 2 parts of beta-mannase, 1 part of protease, 0.4 part of bacillus subtilis, 0.4 part of clostridium butyricum, 2 parts of coated lactic acid bacteria, 10 parts of bentonite (unmodified), 30 parts of benzoic acid, 6 parts of plant essential oil and 44.2 parts of a saccharomyces cerevisiae culture.
Effects of the embodiment
The premix mate biological function bag provided by the invention can improve the intestinal health of laying hens, and production performance tests and verifications are carried out by selecting the halan brown laying hens and old laying hens with unhealthy intestinal tracts as test animals.
Comparison of adsorption Effect of an Experimental mycotoxin adsorbent
The method comprises the steps of adding a premix mate biological function bag into a mildewed feed, adopting the same mildewed feed, detecting that the mildewed feed contains 2.35mg/kg of aflatoxin, dividing the mildewed feed into two groups, repeating each group for three times, using a group A as a mixing example 1, using a group B as a mixing example 4, uniformly mixing, and detecting the adsorption rate of the aflatoxin, wherein specific results are shown in a table 1.
TABLE 1
Experiment on influence of two-premix mate biological function bag on laying hen production performance with poor intestinal health degree
In the experiment, 320 Hailan brown laying hens which are 31 weeks old and have thin excrement, poor formability and yellowing and the phenomenon of material passing are randomly selected to be divided into 2 daily ration treatment groups, each group is 8 in number, and each group is 20 in number. Group a was a control group, fed corn-soybean meal type basal diet according to the recommended formula of premix, and group B added 1 bag of premix mate biofunctional package per ton of basal diet (example 1). The pre-test period was 2 weeks, and the positive test period was 10 weeks. And calculating the average laying rate, average egg weight, average daily feed intake and feed-egg ratio in the positive test period by taking the repetition as a unit. The test results are shown in table 2 (the influence of the premix mate biological function package on the laying hen production performance with poor intestinal health degree).
TABLE 2
As can be seen from table 2, the addition of 1 bag of the premix mate biofunction bag to the basic ration can significantly improve the average laying rate and average egg weight of the laying hens, significantly reduce the average daily feed intake and feed-egg ratio of the laying hens, and significantly improve the production performance of the laying hens. The premix mate biological function bag is proved to improve the intestinal health degree of the laying hens and the digestibility of the intestinal tracts to nutrient substances.
Experiment on influence of three-premix mate biological function bag on production performance of old laying hens
In the experiment, 320 55-week-old Hailan brown laying hens were randomly divided into 2 daily ration treatment groups, each group had 8 replicates, and each replicate had 20 chickens. Group A is a control group, and is fed with corn-soybean meal type basic ration according to the recommended formula of the premix, and group B is added with 1 bag of premix mate biological function bag in each ton of basic ration in the manner of adding every day in the first 4 weeks and stopping for 1 day in the last 6 weeks. The pre-test period was 2 weeks, and the positive test period was 10 weeks. And calculating the average laying rate, average egg weight, average daily feed intake and feed-egg ratio in the positive test period by taking the repetition as a unit. The test results are shown in table 3 (effect of premix mate biofunctional package on production performance of old layer chicken).
TABLE 3
As can be seen from table 3, adding 1 bag of premix mate biofunction bag to the basal diet can significantly improve the average laying rate of the old layer chicken, with a tendency to reduce the feed-egg ratio. The premix mate biological function bag is proved to improve the intestinal health degree of the old laying hens and promote the digestibility of the old laying hens on nutrient substances. After 4 weeks, the intestinal tract is kept healthy by adding the feed for 1 day and stopping for 1 day, so that the intestinal tract health of old laying hens can be improved, and the cost can be saved.
Claims (7)
1. The premix mate biological function bag for improving intestinal health of laying hens is characterized by comprising the following raw materials in parts by weight: 1-5 parts of glucose oxidase, 1-5 parts of beta-mannase, 0.5-2 parts of protease, 0.1-1 part of bacillus subtilis, 0.1-1 part of clostridium butyricum, 1-5 parts of coated lactic acid bacteria, 5-15 parts of mycotoxin adsorbent, 10-50 parts of benzoic acid, 1-10 parts of plant essential oil and 30-70 parts of saccharomyces cerevisiae culture.
2. The premix mate biofunctional package of claim 1, wherein in the raw materials: glucose oxidase is 1000 IU/g, beta-mannase is 50000 IU/g, protease is 50000 IU/g, bacillus subtilis is 1000 hundred million/g, clostridium butyricum is 100 hundred million/g, and coated lactic acid bacteria is 100 hundred million/g.
3. The premix mate biofunctional package according to claim 1 or 2, wherein the mycotoxin adsorbent is modified bentonite.
4. The premix mate biofunctional package of claim 3, wherein the modified bentonite is prepared by the following method:
(1) adding acetic acid solution into bentonite, stirring for 30-40min, filtering, and adding saturated glucose solution into the filtered bentonite for infiltration;
(2) soaking for 1h, filtering, and calcining the soaked bentonite.
5. The premix mate biofunctional package according to claim 4, wherein the mass concentration of the acetic acid solution is 16-18%.
6. The premix mate biofunctional package according to claim 4 or 5, wherein the calcination is at 400-450 ℃ for 1.5 h.
7. The use of a premix mate biofunction bag according to any of claims 1-6 in feed for improving the intestinal health of laying hens, wherein when the pulse feeding mode is adopted, the feeding is stopped for 1 day and 2 days, and the feeding is stopped for 1 day and 3 days.
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CN104012804A (en) * | 2014-06-16 | 2014-09-03 | 辽宁禾丰牧业股份有限公司 | Laying period compound premix capable of improving intestinal health of laying hens, and applications of laying period compound premix |
CN112471327A (en) * | 2020-12-02 | 2021-03-12 | 山东和康源集团有限公司 | Preparation method of premix for improving intestinal health of laying hens |
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CN104012804A (en) * | 2014-06-16 | 2014-09-03 | 辽宁禾丰牧业股份有限公司 | Laying period compound premix capable of improving intestinal health of laying hens, and applications of laying period compound premix |
CN112471327A (en) * | 2020-12-02 | 2021-03-12 | 山东和康源集团有限公司 | Preparation method of premix for improving intestinal health of laying hens |
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