CN114540253B - Application of bacillus pumilus P6 keratinase in detergent and application method - Google Patents
Application of bacillus pumilus P6 keratinase in detergent and application method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38609—Protease or amylase in solid compositions only
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
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- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38618—Protease or amylase in liquid compositions only
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- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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Abstract
The invention belongs to the field of washing enzymes of industrial enzyme preparations, and particularly relates to application of a microbacterium P6 keratinase in a detergent and an application method thereof, wherein the strain is preserved in China general microbiological culture Collection center (CGMCC) on 03-01 th 2022, the preservation number is CGMCC No.24453, and the preservation address is No. 3 of Xilu No. 1 Beijing morning of the Korean district in Beijing. The keratinase provided by the invention is obtained by inoculating seed liquid of microbacterium P6 into a feather culture medium for culture, belongs to medium-temperature alkaline protease, has good stress resistance, can tolerate ionic/nonionic surfactants, oxidants and bleaches, can maintain stable enzyme activity for a long time in a commercially available detergent, and has excellent decontamination, descaling and stain removal effects on common stains.
Description
Technical Field
The invention belongs to the field of enzymes for washing industrial enzyme preparations, and particularly relates to a microbacteriumExiguobacterium sp.Application of P6 keratinase in detergent and application method thereof.
Background
Enzyme additives commonly used in the detergent industry include proteases, amylases, and lipases, each of which is suitable for removing different types of soils. Despite the complexity of current detergents, there remains a need to develop new detergents comprising new enzymes/enzyme blends to meet industry needs.
Compared with the common protease, the keratinase can wash away the keratinized waste adhered to the surface of clothes more easily, and can be used as an additive of detergent. The application requires that the protease has good alkali resistance, high activity in a wide temperature range, good cleaning effect on stains, and stability in self-activity against ionic/nonionic surfactants, oxidizing agents, bleaching agents, enzymes, and other components in detergents (Vojcic L et al, new Biotechnol, 2015, 32 (6): 342-348). Although there are many reports that general proteases can be applied to detergent additives, there are few studies on the application of diagonal proteases in this respect. Commercial enzyme Keratoclean PB of keratinase currently reported for the preparation of detergent products developed by Zurko and Proteos Biotech IncKeratinase from Bacillus licheniformis (patent DE 10 2016 214 A1 2018.02.08); in addition, bacillus cereusBacillus cereus The keratinase produced by Y-15 was excellent in removing protein stains (CN 112126536B). The keratinases used for the preparation of detergent products are all from bacteria of the genus Bacillus. As is well known, the catalytic properties, molecular weights, enzymatic properties and the like of keratinase produced by strains of different species may have great differences, and in order to meet the enzyme requirements of the enzyme preparation industry, a novel keratinase resource with potential application value, wider substrate and new enzymolysis characteristics needs to be searched urgently.
The microbacterium is a gram-positive, spore-free, facultative anaerobic bacterium. Research shows that most of the microbacterium can survive in wide temperature, salinity and pH range and produce proteinase, amylase, etc. The method discloses the utilization of metabolic pathways of microbacterium or extracellular enzymes to degrade petroleum hydrocarbon, treat petroleum-polluted water areas or oil refining wastewater (CN 103937723A, CN 110468077A and CN 106754499A), treat protein-containing wastewater (CN 112143671A, CN 110468077A, CN 111454865A, CN 105621626A and CN 110317757757751A), adsorb heavy metals to repair heavy metal-polluted environments (CN 106148239A, CN 112625967A and CN 101215541A), adsorb and degrade pesticides (CN 102181386A), degrade dyes (CN 102268393A) such as malachite green and crystal violet in the environment and the like, thereby greatly inspiring and widening the application range of the microbacterium in the industries of sewage treatment, environment treatment and the like. Novitin discloses the application of M4 family metalloprotease produced by deep sea Microbacterium in preparing clothes washing and tableware detergent (CN 104619838A), which is the application of few microbium proteases in detergent industry, the protease claimed in the invention has the best washing effect on blood/milk/ink, and the specificity of the enzyme on protein substrates such as blood/milk is revealed.
Disclosure of Invention
Against the current situation mentioned in the background, the present invention provides a microorganismExiguobacterium sp.P6 (hereinafter abbreviated as Microbacterium P)6) The application of keratinase in detergents also provides a scheme for applying the keratinase produced by the micro bacillus P6 in the fermentation culture process in the detergents; and a detergent containing the above keratinase.
The novel microbacterium P6 capable of efficiently degrading feather keratin waste can grow in a culture medium with feather as the only energy source and efficiently degrade the feather, and the degradation rate of the strain to the feather is 98% after 48 hours of culture. The strain is preserved in China general microbiological culture Collection center (CGMCC) No.24453 with the strain preservation number of CGMCC No.24453 and is classified and named as microbacteriumExiguobacterium sp.. The keratinase secreted from the bacterial cells has strong degradation capability on feather keratin, and the degradation rate under the experimental condition reaches 85%. The enzyme is proved to belong to intermediate-temperature alkaline keratinase, has good stress resistance, can tolerate ionic/nonionic surfactants, oxidants and bleaches, can maintain stable enzyme activity for a long time in a commercial detergent, has excellent decontamination effect on ink and iodine, and has great application potential in preparation of decontamination products.
The keratinase is obtained by the following method:
inoculating the seed solution of the microbacterium P6 into a feather culture medium according to the inoculation amount of 1-3% (volume), culturing at 25 ℃ and 180 r/min for 48 h, and centrifuging to remove thalli to obtain the keratinase. Culture medium formula (g/L): feather 15, naCl 0.5, K 2 HPO 4 1.0，KH 2 PO 4 0.4，MgCl 2 ·7H 2 O 0.1，CaCl 2 0 .06。
Preferably, the inoculation is carried out with an inoculum size of 1 to 3% by volume, preferably 2%.
The use of a formulation comprising any of the following in a detergent is also within the scope of the invention:
the fermentation liquor of the micro bacillus P6 with the thallus removed;
or, keratinase/purified enzyme preparation obtained by fermentation of Microbacterium P6;
or, an extract/metabolite of microbacterium P6;
in the specific application process of the microbacterium P6 keratinase, the enzyme is diluted or is not diluted and is directly mixed with a detergent, and then the mixture is used for removing various stains or dirt. The effect is far better than that of only using a single detergent.
The preparation method of the keratinase comprises the following steps: culturing the microbacterium P6 in a feather culture medium for 48 to 72 hours to obtain a fermentation liquid, centrifuging the fermentation liquid at 11000 rpm for 15 minutes, and collecting the supernatant of the fermentation liquid to obtain the keratinase.
The invention also provides a detergent containing the above keratinase and/or purified enzyme solution. The keratinase obtained by the method has the action temperature of 45-55 ℃ and the pH value of 8.5-9.5; preferably, the optimum temperature is 50 ℃ and the pH is 9.0.
The keratinase has remarkable effect when being applied to washing and/or detergent products, particularly to stains containing keratinase, and the application effect of purified keratinase and enzyme liquid or enzyme preparations after purification in washing and/or detergent products is excellent. The above improvements are also the focus of the protection of the present invention.
The invention also provides the use of a detergent for removing keratin-containing stains, wherein the detergent comprises any one of the following components:
the fermentation liquor of the micro bacillus P6 with the thallus removed;
or, keratinase/purified enzyme preparation obtained by fermentation of Microbacterium P6;
or, an extract/metabolite of microbacterium P6;
the preservation number of the Microbacterium P6 strain is CGMCC No. 24453. The enzyme preparation is keratinase or purified keratinase.
Compared with the prior art, the invention has the following advantages:
the invention provides a microbacterium P6, which can degrade keratin and has excellent application effect in detergents. The inventor conducts a large amount of search and reference, and no report of keratin degrading bacteria and keratinase from the species exists at present, and no report of applying the microbial inoculum/enzyme preparation to detergents exists.
The inventor proves that the keratinase produced by the strain has a very good degradation effect on feather keratin through experiments, which shows that the novel keratinase degrading bacteria of the invention can produce a novel keratinase, have a novel enzymolysis characteristic, and have great application potential in the aspect of efficiently converting livestock and poultry wastes into washing and descaling products.
Drawings
FIG. 1: the keratin is taken as a substrate, and the optimum catalytic enzyme activity temperature (A) and the optimum pH (B) of the keratinase are adopted;
FIG. 2: stability of keratinase in detergent (1%);
FIG. 3: the detergent effect of keratinase as detergent additive on ink and iodine.
Detailed Description
The invention is further described below in conjunction with specific embodiments, and the advantages and features of the invention will become more apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and substitutions are intended to be within the scope of the invention.
Example 1
Extraction of P6 keratinase of Microbacterium
The method for obtaining the P6 keratinase of the microbacterium comprises the following steps: inoculating the seed solution of the microbacterium P6 into a feather culture medium according to the inoculation amount of 2%, culturing at 25 ℃ at 180 r/min for 48 h, and centrifuging to remove thalli to obtain keratinase. Culture medium formula (g/L): feather 15, naCl 0.5, K2HPO 4.0, KH2PO4.4, mgCl2.7H2O 0.1, caCl2.06.
The enzyme activity determination method of the keratinase comprises the following steps: reference is made to patent ZL 201689190.2.
Optimum enzyme activity temperature: enzyme solution was diluted to appropriate concentration with Tris-HCl buffer pH 8.5 using 2% keratin as substrate, and mixed with substrate 1:1 and measuring the enzyme activity under the condition of pH 8.5 after mixing. The reaction temperature is 30-65 ℃, one point is measured every 5 ℃, and the highest enzyme activity is taken as 100%;
as a result: keratinase has highest activity of catalyzing keratin substrate at 50 deg.C, and the activity of keratinase at 40-60 deg.C is more than 65% of the highest activity, and belongs to mesophilic protease (figure 1A).
In the washing industry, enzymes are generally required to play a stain removing function at a higher temperature, and the keratinase can keep higher enzyme activity at 40-60 ℃, so that the property provides a good foundation for the application of the keratinase in washing products.
Optimum pH: buffer systems with different pH values, namely Tris-HCl (pH value is 7.0-9.0) and Gly-NaOH (pH value is 9.0-10.0) are prepared. Respectively preparing 2% of keratin substrates by using buffer solution systems with the pH value of 7.0-10.0, diluting enzyme solutions to proper times by using different buffer solution systems, and then measuring the activity of keratinase at 50 ℃ to determine the optimal pH value of the keratinase.
As a result: the keratinase has highest enzyme activity at pH 8.5, can maintain 80% of the highest enzyme activity at pH 8.0-9.5, and belongs to alkaline protease (figure 1B). In industrial production, especially in the application process in the fields of washing, keratin waste degradation, feed processing and the like, the enzyme is usually enabled to play a role under alkaline conditions, so that the industrial enzyme is usually required to keep high activity under alkaline conditions, and the keratinase conforms to the use conditions of the industrial enzyme and has potential application value.
Example 2
The keratinase of the invention is applied to removing stains, and the specific method is as follows:
tolerance of keratinase in detergents: diluting the keratinase to 35U, mixing with 2% of green blue detergent 1 by mass percent, and measuring residual enzyme activity by utilizing a Folin-phenol method after keeping the temperature for 6 h and 12 h at room temperature. The enzyme activity of keratinase not mixed with detergent when the keratinase is kept for 0 h is taken as 100%.
Washing effect of keratinase on stains: the concentration of the Billang detergent is 1%, 35U of enzyme is added, and cotton cloth stained with stains is cut into a proper size. Washing conditions are as follows: washing at 37 ℃ and 120 rpm for 1 h. The washing effect of the green wave detergent without the enzyme solution was compared with that of distilled water.
The keratinase keeps stable after being preserved for 12 h in 1% of green wave detergent (figure 2), and compared with green wave detergent without keratinase with the mass fraction of 1%, the detergent added with the keratinase has better washing effect when washing stains such as ink, iodine and the like (figure 3). The above results indicate that the keratinase has potential application in the development of detergent additives.
Compared with the common protease, the keratinase can wash away keratinized waste adhered to the surface of clothes more easily, and can be used as an additive of detergent. The application requires that the protease has good alkali resistance, can still maintain high activity in a wider temperature range, has good washing effect on stains, can resist ionic/nonionic surfactants, oxidizing agents, bleaching agents, enzymes and other components in the detergent and keeps stability of self activity. Although there are many reports that general proteases can be applied to detergent additives, there are few studies on the application of diagonal proteases in this respect. In view of this problem, the keratinase preparation provided by the invention belongs to alkaline protease, and is suitable for being used in detergents under alkaline conditions; the optimum pH value is 8.5, the optimum pH value is 8.0-9.5, 80% of the highest enzyme activity is kept, the enzyme can maintain high activity in a wider temperature range, and the optimum enzyme activity temperature is 50 ℃. The enzyme has good stress resistance, the enzyme activity can still be kept stable after 12 hours of heat preservation in a commercial detergent, and compared with the Bila laundry detergent without adding keratinase and with the mass fraction of 1%, the detergent with the enzyme has better washing effect when washing stains such as ink, iodine and the like. The above results indicate that the keratinase has potential application in the development of detergent additives.
Claims (8)
1. MicrobacteriumExiguobacterium sp.The keratinase produced by P6 fermentation is applied in detergent, the microbacterium P6 is preserved in China general microbiological culture Collection center on 03-01 th 2022 month, the preservation number of the strain is CGMCC No.24453, and the classification name is microbacteriumExiguobacterium sp.The preservation address is No. 3 Xilu No. 1 of Beijing, chaoyang, the region of Chaoyang.
2. The use according to claim 1, wherein the micro-bacillus P6 is fermented to obtain keratinase, and the fermentation medium is prepared from: feather 15 g/L, naCl 0.5 g/L, K 2 HPO 4 1.0 g/L、KH 2 PO 4 0.4 g/L、MgCl 2 ·7H 2 O 0.1 g/L、CaCl 2 0.06 g/L, and the rest is water.
3. The application as claimed in claim 1, wherein the fermentation medium is cultured at 20-30 ℃ and 180 r/min for 48-72 h.
4. The use according to claim 1, wherein the amount of the microorganism bacterium P6 inoculated is 1 to 3% by volume when the fermentation medium is cultured.
5. The use according to claim 1, wherein the keratinase is a purified enzyme preparation.
6. The use according to claim 1, wherein the keratinase is prepared by a process comprising: and (3) fermenting the microbacterium P6 for 48 to 72 hours to obtain fermentation liquor, centrifuging the fermentation liquor at 11000 rpm for 15 min, and collecting supernatant to obtain the keratinase.
7. The detergent is characterized by containing microbacteriumExiguobacterium sp.Keratinase produced by P6 fermentation and/or purified keratinase, said microbacteriumExiguobacterium sp.The preservation number of the strain of the P6 is CGMCC No. 24453.
8. Use of a detergent for removing keratin-containing stains, said detergent comprising:
keratinase/purified keratinase enzyme preparation obtained by fermentation of Microbacterium P6;
the strain preservation number of the microbacterium P6 is CGMCC No. 24453.
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