CN114532361A - Bacillus firmus granule and preparation method and application thereof - Google Patents
Bacillus firmus granule and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The application relates to the field of granules, and particularly discloses bacillus firmus granules as well as a preparation method and application thereof. The bacillus firmus granule comprises the following raw materials in parts by weight: 0.5-1.5 parts of bacillus firmus mother powder, 5.5-7.5 parts of promoting component, 88-276 parts of carrier, 2-5 parts of wetting agent, 2-5 parts of dispersing aid and 2-5 parts of adhesive; the preparation method comprises the following steps: uniformly mixing the bacillus firmus mother medicine, the promoting component, the wetting agent, the dispersing auxiliary agent and the carrier, adding the binder after crushing, and finally extruding and granulating to obtain the bacillus firmus granules. The bacillus firmus granule can be used for preventing and treating tomato and tobacco root-knot nematodes, and has the advantage of improving the prevention and treatment effect of the bacillus firmus granule on the root-knot nematodes.
Description
Technical Field
The application relates to the field of granules, in particular to bacillus firmus granules and a preparation method and application thereof.
Background
The granule is a solid dosage form obtained by mixing and granulating raw medicines and auxiliary agents such as a carrier, an adhesive, a dispersing agent, a wetting agent, a stabilizing agent and the like. Its performance requirements mainly include fineness, uniformity, storage stability, hardness and disintegration property.
The bacillus firmus is a novel microbial source biological pesticide, after furrow application or root irrigation, live spores of the bacillus firmus utilize nutrition and moisture of crop roots to propagate in root soil, rapidly occupy soil around the whole crop rhizosphere, and achieve the effect of effectively inhibiting and killing root-knot nematode eggs and larvae.
With respect to the above-described related art, the inventors consider that: the growth of the bacillus firmus in the barren soil is inhibited, so that the effect of the bacillus firmus in preventing and controlling the root-knot nematode is poor.
Disclosure of Invention
In order to improve the control effect of the bacillus firmus granules on root-knot nematodes, the application provides bacillus firmus granules and a preparation method and application thereof.
In a first aspect, the present application provides a bacillus firmus granule, which adopts the following technical scheme:
the bacillus firmus granule comprises the following raw materials in parts by weight: 0.5-1.5 parts of bacillus firmus mother powder, 5.5-7.5 parts of promoting component, 88-276 parts of carrier, 2-5 parts of wetting agent, 2-5 parts of dispersing auxiliary agent and 2-5 parts of adhesive, wherein the promoting component comprises thiamine thiocyanate and sodium ethylene diamine tetramethylene phosphonate, and the weight ratio of the thiamine thiocyanate to the sodium ethylene diamine tetramethylene phosphonate is 8-12: 3.
Through adopting above-mentioned technical scheme, the sodium ethylene diamine tetra methylene phosphonate improves the joint strength between bacillus firmus mother powder and thiamine thiocyanate, and after the germicide dissolved in aqueous, the bacillus firmus attached on thiamine thiocyanate, and thiamine thiocyanate decomposes and promotes bacillus firmus to grow, and the near soil of bacillus firmus is hardened to the effective reduction of sodium ethylene diamine tetra methylene phosphonate simultaneously, effectively reduces the harmful effects of soil to bacillus firmus growth, improves the prevention and cure effect of bacillus firmus granule to the root-knot nematode.
Preferably, the weight of the bacillus firmus mother powder accounts for 0.5 percent of the total weight of the granules.
By adopting the technical scheme, the adding amount of the bacillus firmus mother powder is selected, so that the sterilizing effect of the granules is effectively improved.
Preferably, the bacterial content of the bacillus firmus mother powder is 1000 hundred million spores/g.
By adopting the technical scheme, the bacterium content of the prepared bacillus firmus granules is 5 hundred million spores/gram, so that the bacterium content of the granules is more reasonable, and the growth of the bacillus firmus is promoted.
Preferably, the weight ratio of the bacillus firmus mother powder to the thiamine thiocyanate to the Ethylene Diamine Tetraacetic Acid (EDTA) is 2:10: 3.
By adopting the technical scheme, the weight ratio of the bacillus firmus mother powder, the thiamine thiocyanate and the ethylene diamine tetramethylene sodium phosphonate is limited, so that the promotion effect of the thiamine thiocyanate and the ethylene diamine tetramethylene sodium phosphonate on the bacillus firmus is improved.
Preferably, the carrier is attapulgite.
By adopting the technical scheme, the attapulgite is taken as an adsorption carrier, and the bacillus firmus mother powder and the thiamine thiocyanate are adsorbed on the attapulgite through the ethylenediamine tetramethylene phosphonic acid sodium, so that the stability of the granules is effectively improved.
Preferably, the weight ratio of the attapulgite to the thiamine thiocyanate to the sodium ethylene diamine tetramethylene phosphonate is 366:10: 3.
By adopting the technical scheme, the weight ratio of the attapulgite, the thiamine thiocyanate and the ethylene diamine tetra methylene sodium phosphonate is adjusted, the ethylene diamine tetra methylene sodium phosphonate enables the strong bacillus mother powder and the thiamine thiocyanate to be adhered to the attapulgite, the strong bacillus can conveniently absorb nutrient elements provided by the thiamine thiocyanate and the ethylene diamine tetra methylene sodium phosphonate, and accordingly growth of the strong bacillus is promoted.
In a second aspect, the present application provides a method for preparing bacillus firmus granules, which adopts the following technical scheme:
a preparation method of bacillus firmus granules comprises the following steps: uniformly mixing the bacillus firmus mother drug, thiamine thiocyanate, a wetting agent, a dispersing aid and a carrier, crushing, adding ethylene diamine tetra methylene phosphonic acid sodium and a binder, and finally extruding and granulating to obtain the bacillus firmus granules.
In a third aspect, the application provides an application of bacillus firmus granules, which adopts the following technical scheme: use of bacillus firmus granules for preventing and treating tomato and tobacco root-knot nematodes.
In summary, the present application has the following beneficial effects:
1. this application sodium ethylene diamine tetra methylene phosphonate improves the joint strength between the mother powder of bacillus firmus and thiamine thiocyanate, after the germicide dissolved in aqueous, the bacillus firmus is attached to on the thiamine thiocyanate, thiamine thiocyanate decomposes and promotes bacillus firmus to grow, the near soil of bacillus firmus of effective reduction of sodium ethylene diamine tetra methylene phosphonate simultaneously hardens, effectively reduce the harmful effects of soil to the growth of bacillus firmus, improve the prevention and cure effect of bacillus firmus granule to the root knot nematode.
2. The ethylene diamine tetra methylene phosphonic acid sodium in the application enables the strong bacillus mother powder and the thiamine thiocyanate to be adhered to the attapulgite, so that the strong bacillus can absorb the thiamine thiocyanate and the ethylene diamine tetra methylene phosphonic acid sodium to provide nutrient elements for the strong bacillus, and the growth of the strong bacillus is promoted.
Detailed Description
The bacillus firmus mother medicine in the application has the bacterium content of 1000 hundred million spores/g and is purchased from Jiangxi Shunquan biotechnology limited company; thiamine thiocyanate, purchased from Hubei Xin run chemical Co., Ltd; the sodium ethylene diamine tetra (methylene phosphonic acid) is purchased from Shandong Mizhong moisturizing treatment Co., Ltd; the wetting agent is fatty alcohol polyoxyethylene ether purchased from Jinan Yunyan chemical Co., Ltd; the dispersing auxiliary agent is a naphthalene sulfonic acid sodium salt formaldehyde condensation compound purchased from Wuhan daoyer biotechnology limited; the adhesive is carboxymethyl cellulose purchased from; the carrier is attapulgite purchased from Sihehou Yitong Bentonite GmbH.
The present application is further described in detail in connection with the following examples.
Examples
Example 1
S1, uniformly mixing 0.5kg of bacillus firmus mother drug, 3kg of thiamine thiocyanate, 2kg of wetting agent, 2kg of dispersing aid and 88kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 2kg of adhesive, mixing uniformly, and extruding and granulating by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 2
S1, uniformly mixing 0.5kg of bacillus firmus mother drug, 4kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 3
S1, uniformly mixing 0.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 4
S1, uniformly mixing 0.5kg of bacillus firmus mother drug, 6kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 5
S1, uniformly mixing 1kg of bacillus firmus mother drug, 4kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 6
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 7
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 6kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylenediamine tetramethylene phosphonate and 3.5kg of adhesive, mixing uniformly, and carrying out extrusion granulation by an extrusion granulator to obtain the particle size D90420 μm, yielding bacillus firmus granules.
Example 8
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 4kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 9
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of ethylenediamine tetraacetic acid disodium saltMixing sodium thiophosphonate and 3.5kg of adhesive uniformly, extruding and granulating by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 10
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 6kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 11
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 2kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 12
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 13
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 2kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 14
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 15
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 2kg of adhesive, mixing uniformly, and extruding and granulating by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 16
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 5kg of adhesive, mixing uniformly, and extruding and granulating by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 17
S1, uniformly mixing 0.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 88kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 18
S1, uniformly mixing 0.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 19
S1, uniformly mixing 0.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 276kg of carrier, and crushing the mixture by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 20
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 88kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 21
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 276kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 22
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 88kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 23
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 24
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 276kg of carrier, and crushing the mixture by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Example 25
S1, uniformly mixing 1.5kg of bacillus firmus mother drug, 6kg of thiamine thiocyanate, 5kg of wetting agent, 5kg of dispersing aid and 276kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 5kg of adhesive, mixing uniformly, and extruding and granulating by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Comparative example
Comparative example 1
S1, uniformly mixing 1kg of bacillus firmus mother drug, 5kg of thiamine thiocyanate, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using a jet mill to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 3.5kg of adhesive, mixing uniformly, extruding and granulating by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Comparative example 2
S1, uniformly mixing 1kg of bacillus firmus mother medicine, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 1.5kg of sodium ethylene diamine tetra (methylene phosphonic acid) and 3.5kg of adhesive, mixing uniformly, and performing extrusion granulation by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
Comparative example 3
S1, uniformly mixing 1kg of bacillus firmus mother medicine, 3.5kg of wetting agent, 3.5kg of dispersing aid and 183kg of carrier, and crushing by using an airflow crusher to ensure that the fineness of the mixture reaches 400 meshes;
s2, adding 3.5kg of adhesive, mixing uniformly, extruding and granulating by an extrusion granulator to obtain a particle size D90420 μm, yielding bacillus firmus granules.
TABLE 1 EXAMPLES AND COMPARATIVE EXAMPLES raw material Table (kg)
Performance test the effect of the granules prepared in examples 1 to 25 and comparative examples 1 to 3 of the present application on controlling tomato root-knot nematode was tested. Wherein the nematode to be tested is Meloidogyne haplobii, and the crop to be tested is greenhouse tomato (hair powder 802).
The specific test steps are as follows:
s1, root irrigation treatment: and (5) cultivating tomato seedlings in a seedling tray filled with sterilized soil 30 days before the test, selecting the tomato seedlings with consistent growth vigor in the 3-leaf stage, and transplanting the tomato seedlings into the soil with the nematodes. Each 5 plants are taken as a group, granules to be tested are irrigated, 10 parallel groups are arranged in each embodiment or comparative example, and meanwhile, clear water is used for replacing the granules to arrange 10 blank control groups;
and S2, applying the pesticide once every 15 days after transplanting, continuously applying the pesticide for three times, cleaning the tomato roots after the last pesticide application 60, investigating and recording the number of root knots, and counting the prevention and treatment effect.
Calculation of drug efficacy
Grading the disease condition according to the following standards:
level 0 is complete root system without root knots;
grade 1 is that a small amount of root knots exist, and the root necrosis is less than 25%;
grade 2 is that the number of root knots accounts for 26 to 50 percent of the root coefficient;
the grade 3 is that the root knot number accounts for 51 to 75 percent of the root coefficient;
the 4 grade is that the root knots are extremely large and large, and account for 76% -100% of the root coefficient.
Calculating disease index and prevention and treatment effect according to a formula:
disease index ═ Σ (number of plants at each stage × number at the stage)/(total number of plants × 4) × 100;
the preventing and treating effect (%) is (contrast disease index-treatment disease index)/contrast disease index multiplied by 100; the disease indexes and the control effects of each group of examples or comparative examples are averaged.
TABLE 2 statistical table of disease index and prevention and cure effect
By combining the example 6 and the comparative examples 1 to 3 and combining the table 2, the root-knot nematode can be effectively prevented and controlled by compounding the ethylenediamine tetramethylene phosphonic acid sodium, the bacillus firmus mother powder and the thiamine thiocyanate. The reason is mainly as follows: the sodium ethylene diamine tetra methylene phosphonate improves the connection strength between the bacillus firmus mother powder and the thiamine thiocyanate, after the bactericide is dissolved in water, the bacillus firmus is attached to the thiamine thiocyanate, the thiamine thiocyanate is decomposed to promote the growth of the bacillus firmus, meanwhile, the sodium ethylene diamine tetra methylene phosphonate effectively reduces soil hardening near the bacillus firmus, the adverse effect of soil on the growth of the bacillus firmus is effectively reduced, and the effect of improving the control effect of the bacillus firmus granule on the meloidogyne can be seen by combining the embodiment 2-10 and the table 2, and the control effect of the granule on the meloidogyne can be effectively improved by adjusting the compounding ratio of the sodium ethylene diamine tetra methylene phosphonate, the bacillus firmus mother powder and the thiamine thiocyanate.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.
Claims (8)
1. The bacillus firmus granule is characterized by comprising the following raw materials in parts by weight: 0.5-1.5 parts of bacillus firmus mother powder, 5.5-7.5 parts of promoting component, 88-276 parts of carrier, 2-5 parts of wetting agent, 2-5 parts of dispersing auxiliary agent and 2-5 parts of adhesive, wherein the promoting component comprises thiamine thiocyanate and sodium ethylene diamine tetramethylene phosphonate, and the weight ratio of the thiamine thiocyanate to the sodium ethylene diamine tetramethylene phosphonate is 8-12: 3.
2. The bacillus firmus granule according to claim 1, wherein the weight of the bacillus firmus mother powder accounts for 0.5 percent of the total weight of the granule.
3. The bacillus firmus granule as claimed in claim 2, wherein the bacillus firmus mother powder has a bacterial content of 1000 billion spores/g.
4. The Bacillus firmus granule as claimed in claim 3, wherein the weight ratio of the Bacillus firmus mother powder to thiamine thiocyanate to sodium ethylene diamine tetra methylene phosphonate is 2:10: 3.
5. The bacillus firmus granule according to claim 1, wherein the carrier is attapulgite.
6. The Bacillus firmus granule as claimed in claim 6, wherein the weight ratio of the attapulgite, the thiamine thiocyanate and the sodium ethylene diamine tetra methylene phosphonate is 366:10: 3.
7. A method of preparing Bacillus firmus granules according to any of claims 1 to 6, comprising the steps of: uniformly mixing the bacillus firmus mother drug, thiamine thiocyanate, a wetting agent, a dispersing aid and a carrier, crushing, adding ethylene diamine tetra methylene phosphonic acid sodium and a binder, and finally extruding and granulating to obtain the bacillus firmus granules.
8. Use of bacillus firmus granules as claimed in any one of claims 1 to 6, wherein the bacillus firmus granules are used for controlling tomato and tobacco root-knot nematodes.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210332800.8A CN114532361A (en) | 2022-03-31 | 2022-03-31 | Bacillus firmus granule and preparation method and application thereof |
| CN202210917128.9A CN115251084A (en) | 2022-03-31 | 2022-08-01 | Bacillus firmus granule and preparation method and application thereof |
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- 2022-03-31 CN CN202210332800.8A patent/CN114532361A/en not_active Withdrawn
- 2022-08-01 CN CN202210917128.9A patent/CN115251084A/en active Pending
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| CN115251084A (en) | 2022-11-01 |
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