CN114487060A - Polymer microneedle device for detecting glucose - Google Patents
Polymer microneedle device for detecting glucose Download PDFInfo
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- CN114487060A CN114487060A CN202111653875.8A CN202111653875A CN114487060A CN 114487060 A CN114487060 A CN 114487060A CN 202111653875 A CN202111653875 A CN 202111653875A CN 114487060 A CN114487060 A CN 114487060A
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- 229920000642 polymer Polymers 0.000 title claims abstract description 61
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title claims abstract description 29
- 239000008103 glucose Substances 0.000 title claims abstract description 29
- 239000000758 substrate Substances 0.000 claims abstract description 63
- 229910052751 metal Inorganic materials 0.000 claims abstract description 60
- 239000002184 metal Substances 0.000 claims abstract description 60
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 35
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 35
- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 35
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 35
- 229920002120 photoresistant polymer Polymers 0.000 claims abstract description 32
- 238000006243 chemical reaction Methods 0.000 claims abstract description 20
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052802 copper Inorganic materials 0.000 claims abstract description 18
- 239000010949 copper Substances 0.000 claims abstract description 18
- 239000000126 substance Substances 0.000 claims abstract description 8
- 239000010410 layer Substances 0.000 claims description 139
- 210000004369 blood Anatomy 0.000 claims description 17
- 239000008280 blood Substances 0.000 claims description 17
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 239000011241 protective layer Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 claims description 4
- 108010039918 Polylysine Proteins 0.000 claims description 4
- 229920006332 epoxy adhesive Polymers 0.000 claims description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical group [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 4
- 239000010931 gold Substances 0.000 claims description 4
- 229910052737 gold Inorganic materials 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 229920000656 polylysine Polymers 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- FHCPAXDKURNIOZ-UHFFFAOYSA-N tetrathiafulvalene Chemical compound S1C=CSC1=C1SC=CS1 FHCPAXDKURNIOZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000004520 electroporation Methods 0.000 claims description 3
- 230000004927 fusion Effects 0.000 claims description 3
- 238000007731 hot pressing Methods 0.000 claims description 3
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 3
- 239000004626 polylactic acid Substances 0.000 claims description 3
- 239000002861 polymer material Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 230000004044 response Effects 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 3
- 238000002848 electrochemical method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000001028 reflection method Methods 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N tetrahydrofuran Substances C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 239000004593 Epoxy Substances 0.000 description 1
- 206010033372 Pain and discomfort Diseases 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012943 hotmelt Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- -1 potassium ferricyanide Chemical compound 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000004832 voltammetry Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
Abstract
The invention discloses a polymer microneedle device for detecting glucose, comprising: the micro-needle array platform is covered by a plurality of layers of substances and sequentially comprises a conductive medium layer, a glucose oxidase layer, a photoresist layer, a metal layer and a polymer micro-needle substrate for bearing reaction substances from outside to inside; the copper electrode comprises a working electrode and a counter electrode of the micro-needle, and the electrodes are connected by adopting a lead passage. The preparation method is simple, low in cost, good in biocompatibility, high in electrode stability and capable of rapidly improving response time.
Description
Technical Field
The invention relates to the field of glucose concentration detection devices, in particular to a microneedle device for detecting blood glucose concentration and a manufacturing method thereof.
Background
Abnormal blood sugar level (less than 3.6mmol/L or more than 5.8mmol/L) is a sign of diseases such as diabetes, blood sugar monitoring can be implemented to better control blood sugar change of a diabetic patient, and the blood sugar monitoring method has very important guiding significance on the rules of life, activities, exercise, diet and reasonable medication, and can help the patient to find problems at any time and timely go to a hospital for medical treatment.
In order to measure the concentration of glucose in human blood, blood or other body fluids are generally sampled for measurement. The general market of home-use blood glucose meters mainly includes two major categories, namely an electrochemical method and a light reflection technology, wherein the electrochemical method reflects the blood glucose value by adopting the principle of detecting a current signal generated in the reaction process, the light reflection method reflects the blood glucose value by detecting the color change of a test strip in the reaction process, and both the electrochemical method and the light reflection method need to sample human blood for detection.
However, taking blood to detect glucose concentration by blood sampling for skin puncture of a human body (see prior patent document 1) inevitably causes severe pain and discomfort in skin test points of a patient to be tested, and in addition, there are many uncertain factors, the depth and position of the needle determine the amount of bleeding, multiple times of needle insertion are caused, the self-care of the patient is greatly reduced, and the detection of the glucose concentration is inaccurate.
Therefore, it is highly desirable to further improve the efficiency and accuracy of measuring blood glucose concentrations and to greatly improve patient compliance. Patent documents: a full-automatic self-service peripheral blood sugar detector- (CN 202010614054.2).
Disclosure of Invention
In view of the above, the present invention provides a microneedle device for detecting glucose concentration, which has a fast response time and is implanted painlessly, and a method for manufacturing the same.
A microneedle device for detecting glucose concentration in blood, comprising: the micro-needle array platform is covered by a plurality of layers of substances and sequentially comprises a conductive medium layer, a glucose oxidase layer, a photoresist layer, a metal layer and a polymer micro-needle substrate for bearing reaction substances from outside to inside; the copper electrode comprises a working electrode and a counter electrode of the micro-needle, and the electrodes are connected by adopting a lead passage.
The polymer micro-needle substrate of the micro-needle array platform is made of polylactic acid high molecular polymer material and forms the main body part of the micro-needle device.
The metal layer on the microneedle array platform covers the upper layer of the polymer microneedle substrate, and one corner of the metal layer extends to penetrate through the back of the polymer microneedle substrate and is used for transferring electrons during reaction.
The photoresist layer on the microneedle array platform only covers the bottom end of the metal layer needle part of the microneedle array, and the photoresist layer extends to the edge and is tightly attached to the polymer microneedle substrate for fixing the metal layer and insulating the metal layer from the outside.
The glucose oxidase layer on the microneedle array platform covers the metal layer and the upper layer of the photoresist layer and is used for glucose in blood to participate in reaction.
The conductive medium layer on the microneedle array platform covers the upper layer of the glucose oxidase layer and is used for protecting the glucose oxidase layer.
And a part of the metal layer on the microneedle array platform penetrates through the polymer microneedle substrate to be connected with the working electrode on the back and is fixed on the substrate, and the circular copper plate on the microneedle array platform penetrates through the polymer microneedle substrate to be connected with the counter electrode on the back of the polymer microneedle substrate and is fixed on the substrate.
Working electrode and counter electrode at the back of the micro-needle array platform are all designed by bolt holes, are easier to measure the access of voltammetry meter of current in reaction and are all copper with higher conductivity.
The metal layer material of the microneedle array platform was gold.
The photoresist layer of the microneedle array platform is a photoresist material based on a photosensitive resin.
The glucose oxidase layer of the microneedle array platform is a reaction layer formed by mixing and drying PBS solution, polylysine and glucose oxidase.
The conductive protective layer of the microneedle array platform is a mixture of tetrathiafulvalene, urethane, epoxy adhesive, THF, surfactant and the like.
A method of manufacturing a microneedle device for detecting a glucose polymer, comprising:
(1) the polymer micro-needle substrate of the micro-needle array platform is prepared by a fusion hot-pressing method;
(2) forming a metal layer on a polymer microneedle substrate layer on a microneedle array platform;
(3) forming a photoresist layer at the bottom end of the metal layer on the microneedle array platform;
(4) forming a glucose oxidase layer on the metal layer on the microneedle array platform;
(5) forming a conductive medium protective layer on the glucose oxidase layer and the photoresist layer on the microneedle array platform;
(6) and a working electrode and a counter electrode are respectively formed at two ends of the back of the polymer micro-needle substrate on the micro-needle array platform, and a copper plate is formed on the front of the polymer micro-needle substrate.
The copper plate on the front surface of the polymer microneedle substrate is connected with the counter electrode through the polymer microneedle substrate, and the metal layer of the polymer microneedle substrate is connected with the working electrode through the polymer microneedle substrate.
The polymer micro-needle substrate is used for connecting holes of the electrodes and is formed by electroporation.
According to an aspect of the present invention, there is provided a microneedle device for detecting glucose, comprising:
the micro-needle array platform is covered by a plurality of layers of substances and sequentially comprises a conductive medium layer, a glucose oxidase layer, a photoresist layer, a metal layer and a polymer micro-needle substrate for bearing reaction substances from outside to inside; the copper electrode comprises a working electrode and a counter electrode of the micro-needle, and the electrodes are connected by adopting a lead passage.
Preferably, the polymer microneedle substrate of the microneedle array platform is made of polylactic acid high molecular polymer material, and forms a main body part of the microneedle device
Preferably, the metal layer on the microneedle array platform covers the polymer microneedle substrate, and one corner of the metal layer extends through the back of the polymer microneedle substrate for transferring electrons during reaction.
Preferably, the photoresist layer on the microneedle array platform only covers the bottom end of the metal layer needle part of the microneedle array, and the photoresist layer extends to the edge to be tightly attached to the polymer microneedle substrate for fixing the metal layer and insulating the metal layer from the outside.
Preferably, the glucose oxidase layer on the microneedle array platform covers the metal layer and the photoresist layer for the glucose in blood to participate in reaction.
Preferably, the conductive medium layer on the microneedle array platform covers the upper layer of the glucose oxidase layer and is used for protecting the glucose oxidase layer.
Preferably, a part of the metal layer on the microneedle array platform passes through the polymer microneedle substrate to be connected with the back working electrode and fixed on the substrate, and the circular copper plate on the microneedle array platform passes through the polymer microneedle substrate to be connected with the back counter electrode of the polymer microneedle substrate and fixed on the substrate.
Preferably, the working electrode and the counter electrode on the back of the microneedle array platform are designed by bolt holes, so that a voltammeter for measuring current during reaction can be connected more easily, and the working electrode and the counter electrode are both made of copper with higher conductivity.
Preferably, the metal layer material of the microneedle array platform is gold.
Preferably, the photoresist layer of the microneedle array platform is a photoresist material based on a photosensitive resin.
Preferably, the glucose oxidase layer of the microneedle array platform is a reaction layer formed by mixing and drying a PBS solution, polylysine and glucose oxidase.
Preferably, the conductive protective layer of the microneedle array platform is a mixture of tetrathiafulvalene, urethane, epoxy adhesive, THF, surfactant, and the like.
According to another aspect of the present invention, there is provided a method of manufacturing a glucose testing microneedle device, comprising preparing a polymer microneedle substrate of a microneedle array platform by a fusion hot pressing method; forming a metal layer on a polymer microneedle substrate layer on a microneedle array platform; forming a photoresist layer at the bottom end of the metal layer on the microneedle array platform; forming a glucose oxidase layer on the metal layer on the microneedle array platform; forming a conductive medium protective layer on the glucose oxidase layer and the photoresist layer on the microneedle array platform; and a working electrode and a counter electrode are respectively formed at two ends of the back of the polymer micro-needle substrate on the micro-needle array platform, and a copper plate is formed on the front of the polymer micro-needle substrate.
Preferably, the copper plate on the front surface of the polymer microneedle substrate is connected with the counter electrode through the polymer microneedle substrate, and the metal layer of the polymer microneedle substrate is connected with the working electrode through the polymer microneedle substrate.
Preferably, the polymer microneedle substrate is used for connecting the holes of the electrodes, and the holes are punched by electroporation.
The polymer micro-needle substrate is innovatively stacked with the metal layer, the photoresist layer, the glucose oxidase layer and the conductive medium protection layer, the polymer micro-needle is modified through the multilayer film, the defect of large volume of a single needle is overcome, the contact area of a component and a working part is increased, the metal layer is high in conductive efficiency, the electrode is connected with the metal layer, and the response time is greatly prolonged.
Drawings
The above and other objects, features, and advantages of the present invention will become more apparent from the following description of embodiments of the present invention with reference to the accompanying drawings, in which:
fig. 1 is a perspective view of a microneedle device for detecting glucose according to an embodiment of the present invention.
Fig. 2 is a cross-sectional view of a microneedle according to an embodiment of the present invention.
Fig. 3 is a flow chart of fabricating the microneedles according to an embodiment of the present invention.
Detailed Description
Several preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings, but the present invention is not limited to these embodiments. The invention is intended to cover alternatives, modifications, equivalents and alternatives which may be included within the spirit and scope of the invention.
In the following description of the preferred embodiments of the present invention, specific details are set forth in order to provide a thorough understanding of the present invention, and it will be apparent to those skilled in the art that the present invention may be practiced without these specific details.
Fig. 1 is a perspective view of a microneedle device for detecting glucose according to an embodiment of the present invention. The copper plate 230 is fixed on the microneedle array platform 100, the counter electrode 210 is formed on the other side of the microneedle array platform 100, passes through the microneedle array platform 100 and is connected with the copper plate 230, and the working electrode 220 passes through the microneedle array platform 100 and is connected with the metal layer 120.
Fig. 2 is a cross-sectional view of a single microneedle according to an embodiment of the present invention.
The microneedle array platform 100 includes a polymer microneedle substrate 110, a metal layer 120, a photoresist layer 130, a glucose oxidase layer 140, a conductive medium protection layer 150, and a working electrode 160 fixed beside the microneedle array 100. In a preferred embodiment, the photoresist layer 130 covers only the needle bottom of the metal layer 120 for fixing the metal layer 120. The glucose oxidase layer 140 is also coated only on the needle portion of the metal layer 120 as a reaction working region.
The metal layer 120 is used as a main electron transfer region between the glucose oxidase layer 140 and the photoresist layer 130, and the microneedle array platform 100 has 20 microneedles with the same size. The metal layer 120 forms a working electrode that transfers the electrons that the glucose oxidase layer 140 participates in the reaction to a working meter for measuring potential by virtue of its ultra-high conductivity.
The conductive medium protection layer 150 completely covers the upper layer of the microneedle array platform 100. By immobilizing and protecting the glucose oxidase layer 140 from external oxides, the protective layer dissolves under operating conditions, allowing the glucose oxidase layer 140 to participate in the reaction.
Fig. 3 is a flow chart of fabricating the microneedles according to an embodiment of the present invention.
In step S01, the microneedle substrate 110 is prepared by hot melt pressing, the microneedle array 110 is conformally metallized with metal, and the region is connected to the working electrode 220 through the microneedle array platform 100 with silver epoxy to form a wire path.
In step S02, a fourth surface metal layer 120 is added to the microneedle substrate 110. The microneedles were washed with a 0.5m/L sulfuric acid solution to reduce the resistance of the metal coating in step S01, and were plated with a full bright cyanide-free gold plating solution, and the adhesion of the metal to the metal pattern layer 120 of the microneedle substrate 110 was confirmed with a PBS solution containing 1moL/L of potassium ferricyanide.
In step S03, the third surface photoresist layer 130 is covered on the fourth surface metal layer 120. The metal layer 120 is completely covered with photoresist and a portion of the microneedle array platform 100 is dissolved with an acetone solution to expose the metal layer portion of the microneedle array.
In step S04, a second glucose oxidase layer is added to the fourth surface metal layer (exposed metal portion). The microneedle portion was immersed in a solution containing 0.1moL/LPBS, 0.001% polylysine and 10mg/L glucose oxidase and maintained at 4 ℃ overnight.
In step S05, a first conductive medium protective layer is added over the second glucose oxidase layer and the third photoresist layer. Mixing tetrathiafulvalene, 0.5m/L ethyl carbamate, 17.8mg epoxy adhesive, 4mL of HF and 1mL of surfactant, coating the mixture on the surface of a microneedle array, and standing the microneedle array for 20min at normal temperature to form a protective film.
Claims (10)
1. A polymeric microneedle device for detecting glucose, characterized by: comprises a micro-needle array platform and a copper electrode; the microneedle array is covered by a plurality of layers of substances and sequentially comprises a conductive medium layer, a glucose oxidase layer, a photoresist layer, a metal layer and a polymer microneedle substrate for supporting reaction substances from outside to inside; the copper electrode comprises a working electrode and a counter electrode of the micro-needle, and the electrodes are connected by adopting a lead passage.
2. A polymeric microneedle device for detecting glucose according to claim 1, wherein: the polymer microneedle substrate of the microneedle array platform is made of polylactic acid high-molecular polymer material, and forms a main body part of the polymer microneedle device.
3. A polymeric microneedle device for detecting glucose according to claim 1, wherein: the metal layer on the microneedle array platform covers the upper layer of the polymer microneedle substrate, and one corner of the metal layer extends to penetrate through the back of the polymer microneedle substrate and is used for transferring electrons during reaction.
4. A polymeric microneedle device for detecting glucose according to claim 1, wherein: the photoresist layer on the microneedle array platform only covers the bottom end of the metal layer needle part of the microneedle array, and the photoresist layer extends to the edge and is tightly attached to the polymer microneedle substrate for fixing the metal layer and insulating the metal layer from the outside.
5. A polymeric microneedle device for detecting glucose according to claim 1, wherein: the glucose oxidase layer on the microneedle array platform covers the metal layer and the upper layer of the photoresist layer and is used for glucose in blood to participate in reaction; the conductive medium layer on the microneedle array platform covers the upper layer of the glucose oxidase layer and is used for protecting the glucose oxidase layer;
a part of the metal layer on the microneedle array platform penetrates through the polymer microneedle substrate to be connected with the working electrode on the back and is fixed on the substrate, and the circular copper plate on the microneedle array platform penetrates through the polymer microneedle substrate to be connected with the counter electrode on the back of the polymer microneedle substrate and is fixed on the substrate; the working electrode and the counter electrode on the back of the microneedle array platform are designed by bolt holes.
6. A polymeric microneedle device for detecting glucose according to claim 1, wherein: the metal layer material of the micro-needle array platform is gold;
the photoresist layer of the micro-needle array platform is made of photoresist materials based on photosensitive resin;
the glucose oxidase layer of the microneedle array platform is a reaction layer formed by mixing and drying PBS solution, polylysine and glucose oxidase.
7. The glucose-detecting polymeric microneedle device according to claim 1, wherein the conductive protection layer of the microneedle array platform is a mixture of tetrathiafulvalene, urethane, epoxy adhesive, THF, and a surfactant.
8. A method for manufacturing a device for detecting a glucose polymer microneedle according to any one of claims 1 to 7, comprising:
(1) the polymer microneedle substrate of the microneedle array platform is prepared by a fusion hot pressing method;
(2) forming a metal layer on a polymer microneedle substrate layer on a microneedle array platform;
(3) forming a photoresist layer at the bottom end of the metal layer on the microneedle array platform;
(4) forming a glucose oxidase layer on the metal layer on the microneedle array platform;
(5) forming a conductive medium protective layer on the glucose oxidase layer and the photoresist layer on the microneedle array platform;
(6) and a working electrode and a counter electrode are respectively formed at two ends of the back of the polymer micro-needle substrate on the micro-needle array platform, and a copper plate is formed on the front of the polymer micro-needle substrate.
9. The manufacturing method according to claim 8, characterized in that: the copper plate on the front surface of the polymer microneedle substrate is connected with the counter electrode through the polymer microneedle substrate, and the metal layer of the polymer microneedle substrate is connected with the working electrode through the polymer microneedle substrate.
10. The manufacturing method according to claim 8, characterized in that: the polymer micro-needle substrate is used for connecting holes of the electrodes and is formed by electroporation.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR200381448Y1 (en) * | 2005-01-13 | 2005-04-11 | 리노공업주식회사 | Probe Device with Micro-Pin Inserted in Interface Board |
| US20110237925A1 (en) * | 2010-03-26 | 2011-09-29 | Ruifeng Yue | Microneedle array chip, device and patch for transdermal drug delivery utilizing the same, and preparation method therof |
| CN104321105A (en) * | 2012-04-25 | 2015-01-28 | 帝人株式会社 | Microneedle and microneedle array |
| CN105126242A (en) * | 2015-07-26 | 2015-12-09 | 北京化工大学 | Polymer coating microneedle patch convenient for antibiotic skin testing and preparation method thereof |
| CN105228522A (en) * | 2013-03-14 | 2016-01-06 | 萨诺智能公司 | Microsensor on the human body of biological monitoring |
| US20160157764A1 (en) * | 2014-12-03 | 2016-06-09 | IMAST scarl | Microneedle array device and method of making |
| CN107349518A (en) * | 2017-06-22 | 2017-11-17 | 浙江理工大学 | A kind of near infrared light sensitivity microneedle patch and preparation method thereof |
| WO2018053017A1 (en) * | 2016-09-13 | 2018-03-22 | University Of Utah Researchfoundation | Microneedle arrays having a bio-erodible substrate |
| CN110193082A (en) * | 2018-02-27 | 2019-09-03 | 辽宁成大生物股份有限公司 | A kind of preparation method of sterile rabies vaccine coating micropin |
-
2021
- 2021-12-30 CN CN202111653875.8A patent/CN114487060A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR200381448Y1 (en) * | 2005-01-13 | 2005-04-11 | 리노공업주식회사 | Probe Device with Micro-Pin Inserted in Interface Board |
| US20110237925A1 (en) * | 2010-03-26 | 2011-09-29 | Ruifeng Yue | Microneedle array chip, device and patch for transdermal drug delivery utilizing the same, and preparation method therof |
| CN104321105A (en) * | 2012-04-25 | 2015-01-28 | 帝人株式会社 | Microneedle and microneedle array |
| CN105228522A (en) * | 2013-03-14 | 2016-01-06 | 萨诺智能公司 | Microsensor on the human body of biological monitoring |
| US20160157764A1 (en) * | 2014-12-03 | 2016-06-09 | IMAST scarl | Microneedle array device and method of making |
| CN105126242A (en) * | 2015-07-26 | 2015-12-09 | 北京化工大学 | Polymer coating microneedle patch convenient for antibiotic skin testing and preparation method thereof |
| WO2018053017A1 (en) * | 2016-09-13 | 2018-03-22 | University Of Utah Researchfoundation | Microneedle arrays having a bio-erodible substrate |
| CN107349518A (en) * | 2017-06-22 | 2017-11-17 | 浙江理工大学 | A kind of near infrared light sensitivity microneedle patch and preparation method thereof |
| CN110193082A (en) * | 2018-02-27 | 2019-09-03 | 辽宁成大生物股份有限公司 | A kind of preparation method of sterile rabies vaccine coating micropin |
Non-Patent Citations (1)
| Title |
|---|
| JAKUB TRZEBINSKI 等: "Microflfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications", LAB ON A CHIP, vol. 12, no. 2, pages 348 - 352 * |
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