CN114486689B - Blood cell pulse counting error correction method and correction device - Google Patents

Blood cell pulse counting error correction method and correction device Download PDF

Info

Publication number
CN114486689B
CN114486689B CN202210392441.5A CN202210392441A CN114486689B CN 114486689 B CN114486689 B CN 114486689B CN 202210392441 A CN202210392441 A CN 202210392441A CN 114486689 B CN114486689 B CN 114486689B
Authority
CN
China
Prior art keywords
pulse
pulse signal
waveform
reference pulse
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210392441.5A
Other languages
Chinese (zh)
Other versions
CN114486689A (en
Inventor
陈鹏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Yilanbei Biotechnology Co ltd
Original Assignee
Nanjing Yilanbei Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Yilanbei Biotechnology Co ltd filed Critical Nanjing Yilanbei Biotechnology Co ltd
Priority to CN202210392441.5A priority Critical patent/CN114486689B/en
Publication of CN114486689A publication Critical patent/CN114486689A/en
Application granted granted Critical
Publication of CN114486689B publication Critical patent/CN114486689B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1012—Calibrating particle analysers; References therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a hemocyte pulse counting error correction method which comprises the steps of collecting reference pulse signals generated when a plurality of standard particles continuously pass through a gem hole under a constant pressure state; storing the reference pulse signal according to the acquisition time, extractingJudging whether the reference pulse signal is effective or not according to the pulse waveform of the reference pulse signal; extracting effective reference pulse signal, calibrating error of gem hole, and calculating calibration coefficient
Figure 558974DEST_PATH_IMAGE001
(ii) a Collecting actual pulse signals of blood cells passing through the gem hole, and extracting pulse waveforms of the actual pulse signals; using calibration coefficients
Figure 373215DEST_PATH_IMAGE001
The falling edge pulse waveform of the actual pulse signal is re-fitted. The method comprises the steps of firstly collecting the pulse waveform of the standard particles, calculating the correction coefficient of the gem hole by analyzing the pulse waveform of the standard particles, refitting the actually collected blood cell pulse waveform by using the correction coefficient, and accurately correcting pulse amplitude data, thereby greatly improving the accuracy and reliability of blood cell counting.

Description

Blood cell pulse counting error correction method and correction device
Technical Field
The invention belongs to the technical field of signal analysis, identification and processing, and particularly relates to a blood cell pulse counting error correction method and a correction device.
Background
In the aspect of medical hematology detection, a detection scheme based on the coulter principle is commonly used, the principle is that when particles suspended in electrolyte pass through a small-hole tube along with the electrolyte, the same volume of electrolyte is replaced, the resistance between two points inside and outside the small-hole tube is subjected to transient change in a constant-current designed circuit, potential pulses are generated, and the size and the frequency of pulse signals are in direct proportion to the size and the number of the particles. The current blood analyzer also performs detection according to the method, but the detection is accurate, a single cell is required to pass through the axial center area of the small hole in a straight line, the voltage amplitude of the Coulter principle can accurately reflect the size of the cell under the condition, and the volume of the cell is inaccurate when the single cell passes through other paths of the small hole or a plurality of cells simultaneously pass through the small hole area.
In order to solve the above problems, it is common to use a sheath flow impedance method, i.e. a sheath flow cell with hydrodynamic focusing and directional flow, so that the cells can pass through the small holes in turn without gathering the cells through the counting cell, but the disadvantages of this method are obvious, the structure is complex and the cost is high.
In order to solve the above problem, another method is to use a software algorithm for calibration, but at present, an algorithm for correcting the pulse amplitude data by using rising edge pulse data and falling edge data which are not interfered is used, but in actual use, the pulse signal is in an asymmetric form due to different processing errors of the gem hole, different shapes of the gem hole and the like, that is, the rising edge time and the falling edge time are greatly different, and the algorithm cannot accurately correct the pulse amplitude data.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a blood cell pulse counting error correction method, which aims to solve the problem that the pulse amplitude data cannot be corrected accurately due to large time difference between the rising edge and the falling edge of a pulse waveform in the correction method in the prior art.
The invention is realized by adopting the following technical scheme:
a blood cell pulse counting error correction method comprises the following steps:
collecting reference pulse signals generated when a plurality of standard particles continuously pass through a gem hole under a constant pressure state;
storing the reference pulse signal according to the acquisition time, extracting the pulse waveform of the reference pulse signal, and judging whether the reference pulse signal is effective;
extracting effective reference pulse signal, calibrating error of gem hole, and calculating calibration coefficient
Figure 779690DEST_PATH_IMAGE001
；
Collecting actual pulse signals of blood cells passing through the gem hole, and extracting pulse waveforms of the actual pulse signals;
using calibration coefficients
Figure 805153DEST_PATH_IMAGE002
The falling edge pulse waveform of the actual pulse signal is re-fitted.
In order to optimize the technical scheme, the specific measures adopted further comprise:
further, the standard particles are latex spherical substances.
Further, storing the reference pulse signal according to the acquisition time, extracting the pulse waveform of the reference pulse signal, and judging whether the reference pulse signal is effective specifically as follows:
acquiring the maximum value of a sampling signal corresponding to the rising edge of the pulse waveform;
setting the starting point of the rising edge of the pulse waveform according to 5% of the maximum value of the sampling signal
Figure 116048DEST_PATH_IMAGE003
And the end point of the falling edge
Figure 885159DEST_PATH_IMAGE004
Selecting a starting point
Figure 902793DEST_PATH_IMAGE005
And a termination point
Figure 177786DEST_PATH_IMAGE004
Taking the pulse waveform as a detection waveform;
judging whether the reference pulse signal is an effective pulse signal according to the detected waveform, and determining whether the reference pulse signal is an effective pulse signal when the start point of the detected waveform
Figure 428507DEST_PATH_IMAGE003
End point of
Figure 266888DEST_PATH_IMAGE004
And pulse width
Figure 545554DEST_PATH_IMAGE006
All the pulse waveforms are in accordance with the judgment condition, and the pulse waveforms are judged to be effective reference pulse signals,
Figure 443977DEST_PATH_IMAGE007
wherein:
Figure 463886DEST_PATH_IMAGE003
indicating the starting point of the rising edge of the pulse waveform,
Figure 341843DEST_PATH_IMAGE004
indicating the end point of the falling edge of the pulse waveform,
Figure 52179DEST_PATH_IMAGE006
which represents the pulse width of the pulse waveform,
Figure 590346DEST_PATH_IMAGE008
representing the maximum value of the sampled signal.
Further, collecting the effective reference pulse signal, calibrating the error of the gem hole, and calculating a calibration coefficient
Figure 35234DEST_PATH_IMAGE002
The method comprises the following specific steps:
arranging the effective reference pulse signals according to the acquisition time sequence and marking the effective reference pulse signals as
Figure 356362DEST_PATH_IMAGE009
，
Figure 875199DEST_PATH_IMAGE010
Representing the number of valid reference pulse signals;
calculating data from rising edge to falling edge of each effective reference pulse signal
Figure 138863DEST_PATH_IMAGE011
；
Calculating calibration coefficients
Figure 743151DEST_PATH_IMAGE002
The specific calculation is as follows,
Figure 618703DEST_PATH_IMAGE013
Figure 225003DEST_PATH_IMAGE015
…
Figure 403174DEST_PATH_IMAGE016
Figure 134239DEST_PATH_IMAGE017
Figure 79061DEST_PATH_IMAGE018
。
further, using the calibration coefficients
Figure 838070DEST_PATH_IMAGE002
The step of re-fitting the falling edge pulse waveform of the actual pulse signal is specifically as follows:
acquiring sampling data of actual pulse signal
Figure 826623DEST_PATH_IMAGE019
；
Acquiring sampling data of actual pulse signal falling edge from peak value to end point
Figure 61295DEST_PATH_IMAGE020
；
Using calibration coefficients
Figure 294962DEST_PATH_IMAGE002
Calibrating the sampling data of the actual pulse signal to obtain the corrected sampling data of the pulse signal
Figure 938170DEST_PATH_IMAGE021
The specific calibration process is as follows,
Figure 848358DEST_PATH_IMAGE022
Figure 321058DEST_PATH_IMAGE023
Figure 607683DEST_PATH_IMAGE024
…
Figure 715185DEST_PATH_IMAGE025
Figure 671640DEST_PATH_IMAGE026
Figure 880904DEST_PATH_IMAGE027
…
Figure 548384DEST_PATH_IMAGE028
Figure 277437DEST_PATH_IMAGE029
Figure 998268DEST_PATH_IMAGE030
。
a blood cell pulse counting error correction device comprises,
the acquisition module I is used for acquiring a reference pulse signal generated by standard particles passing through the gem hole and sending the reference pulse signal to the storage module I;
the acquisition module II is used for acquiring actual pulse signals generated by blood cells passing through the gem hole and sending the actual pulse signals to the storage module II;
the identification module is used for identifying a valid reference pulse signal in the first storage module;
the calculation module is used for calculating a calibration coefficient according to the ratio of the sampling data of the rising edge and the falling edge of the effective reference pulse signal;
and the calibration module is used for re-fitting the pulse waveform of the actual pulse signal according to the calibration coefficient.
The invention has the beneficial effects that:
compared with the prior art, the method for correcting the pulse counting error of the blood cells comprises the steps of firstly collecting the pulse waveform of the standard particles, calculating the correction coefficient of the gem hole by analyzing the pulse waveform of the standard particles, refitting the actually collected pulse waveform of the blood cells by using the correction coefficient, and accurately correcting pulse amplitude data, so that the accuracy and the reliability of the blood cell counting are greatly improved.
Drawings
Fig. 1 is a flowchart of a method for correcting a blood cell pulse count error according to a first embodiment of the present invention.
Fig. 2 is a pulse waveform diagram of a falling edge pulse signal abnormality.
Fig. 3 is a diagram of an uncalibrated pulse waveform.
Fig. 4 is a diagram of the pulse waveform after calibration.
Fig. 5 is a block diagram showing a connection relationship between blocks of a blood cell pulse count error correction apparatus according to a second embodiment of the present invention.
Fig. 6 is a schematic structural diagram of a network-side server according to a third embodiment of the present invention.
Detailed Description
In order to clarify the technical solutions and operating principles of the present invention, the present invention is further described in detail with reference to specific embodiments in the following drawings, and it should be noted that, without conflict, any combination between the embodiments described below or between the technical features may form a new embodiment.
The first embodiment:
the invention provides a blood cell pulse counting error correction method as shown in figures 1-4, comprising the following steps:
step S1: and acquiring a reference pulse signal generated by a plurality of standard particles continuously passing through the gem hole under a constant pressure state.
Specifically, the method comprises the following steps: the standard particles are latex spherical substances with known volume sizes and negligible errors, the characteristics of the standard particles are consistent with those of blood cells, each standard particle generates a primary pulse signal after passing through the gem hole, a constant flow rate is provided by a negative pressure pump, the amplitude of the pulse generated by the standard particle after passing through the gem hole is within a range of tiny deviation, and the generated pulse signal is used as a reference signal for calibrating the gem hole. The gem hole is assembled by adopting a calibration tool, and the adopted calibration tool is composed of a circulating liquid path with a sheath flow pool.
Step S2: and storing the reference pulse signal according to the acquisition time, extracting the pulse waveform of the reference pulse signal, and judging whether the reference pulse signal is effective.
Specifically, the method comprises the following steps: not less than 200 pulse signals (1 pulse is generated when 1 standard particle passes) are collected as effective counting data, otherwise, the data is not used as reference and needs to be counted again.
S21: acquiring the maximum value of a sampling signal corresponding to the rising edge of the pulse waveform;
s22: setting the starting point of the rising edge of the pulse waveform according to 5% of the maximum value of the sampling signal
Figure 68730DEST_PATH_IMAGE003
And the end point of the falling edge
Figure 182311DEST_PATH_IMAGE004
Selecting a starting point
Figure 546296DEST_PATH_IMAGE003
And a termination point
Figure 952875DEST_PATH_IMAGE004
The pulse waveform in between as the detection waveform;
s23: judging whether the reference pulse signal is an effective pulse signal according to the detected waveform, and determining whether the reference pulse signal is an effective pulse signal when the start point of the detected waveform
Figure 136732DEST_PATH_IMAGE003
End point of
Figure 178637DEST_PATH_IMAGE004
And pulse width
Figure 115238DEST_PATH_IMAGE006
All accord with the judgement condition, judge this pulse waveform as effective benchmark pulse signal, judge specifically as follows:
Figure 443451DEST_PATH_IMAGE031
wherein:
Figure 865336DEST_PATH_IMAGE003
indicating the starting point of the rising edge of the pulse waveform,
Figure 943889DEST_PATH_IMAGE004
indicating the end point of the falling edge of the pulse waveform,
Figure 751308DEST_PATH_IMAGE006
which represents the pulse width of the pulse waveform,
Figure 532313DEST_PATH_IMAGE008
representing the maximum value of the sampled signal.
Step S3: extracting effective reference pulse signal, calibrating error of gem hole, and calculating calibration coefficient
Figure 533505DEST_PATH_IMAGE002
；
S31: arranging the effective reference pulse signals according to the collection time sequence and marking
Figure 307426DEST_PATH_IMAGE032
；
Figure 720084DEST_PATH_IMAGE033
Representing the number of valid reference pulse signals;
s32: calculating data from rising edge to falling edge of each effective reference pulse signal
Figure 967263DEST_PATH_IMAGE034
；
S32: calculating calibration coefficients
Figure 613008DEST_PATH_IMAGE002
The specific calculation is as follows:
Figure 410194DEST_PATH_IMAGE035
Figure 566106DEST_PATH_IMAGE036
…
Figure 938182DEST_PATH_IMAGE038
Figure 556376DEST_PATH_IMAGE039
Figure 672100DEST_PATH_IMAGE040
step S4: collecting actual pulse signals of blood cells passing through the gem hole, and extracting pulse waveforms of the actual pulse signals;
as shown in fig. 3 and 4, the waveform signals generated by different paths of a single cell through the gemstone pore are shown, the paths include three paths a, b and c, specifically, (a) in fig. 3 shows the waveform signal generated by the path a of the single cell through the gemstone pore, (b) in fig. 3 shows the waveform signal generated by the path b of the single cell through the gemstone pore, and (c) in fig. 3 shows the waveform signal generated by the path c of the single cell through the gemstone pore, wherein the waveform signal represented by the path a is a normal waveform signal, and the path b and the path c are both abnormal waveform signals; waveform signals under three paths a, b and c corrected by the method are shown in fig. 4, wherein (a) in fig. 4 represents the waveform signal after a path a of a single cell passing through the gem hole is corrected, (b) in fig. 4 represents the waveform signal after b path b of the single cell passing through the gem hole is corrected, and (c) in fig. 4 represents the waveform signal after c path c of the single cell passing through the gem hole is corrected.
Step S5: using calibration coefficients
Figure 557885DEST_PATH_IMAGE002
The falling edge pulse waveform of the actual pulse signal is re-fitted.
Specifically, the method comprises the following steps: since the data of the rising edge of the pulse signal is not affected, the data of the actual pulse signal before the peak value of the rising edge is the same as the data of the corrected pulse signal, and the data of the pulse after the peak value is affected by the diamond hole, the calculated calibration coefficient is used
Figure 835283DEST_PATH_IMAGE002
Calibration is performed one by one.
S51: acquiring sampling data of actual pulse signal
Figure 940773DEST_PATH_IMAGE041
；
S52: acquiring sampling data of actual pulse signal falling edge from peak value to end point
Figure 860188DEST_PATH_IMAGE042
；
S53: using calibration coefficients
Figure 866059DEST_PATH_IMAGE002
Calibrating the sampling data of the actual pulse signal to obtain the corrected sampling data of the pulse signal
Figure 189724DEST_PATH_IMAGE043
Specifically calibratedThe process is as follows,
Figure 31778DEST_PATH_IMAGE044
Figure 496430DEST_PATH_IMAGE045
Figure 107540DEST_PATH_IMAGE046
…
Figure 743051DEST_PATH_IMAGE047
Figure 180724DEST_PATH_IMAGE048
Figure 441941DEST_PATH_IMAGE050
…
Figure 658290DEST_PATH_IMAGE051
Figure 713970DEST_PATH_IMAGE052
Figure 248726DEST_PATH_IMAGE053
。
the steps of the above methods are divided for clarity, and the implementation may be combined into one step or split some steps, and the steps are divided into multiple steps, so long as the same logical relationship is included, which are all within the protection scope of the present patent; it is within the scope of the patent to add insignificant modifications to the algorithms or processes or to introduce insignificant design changes to the core design without changing the algorithms or processes.
The second embodiment:
as shown in fig. 5, a second embodiment of the present invention provides a blood cell pulse count error correction device including:
the acquisition module I is used for acquiring a reference pulse signal generated by standard particles passing through the gem hole and sending the reference pulse signal to the storage module I;
the acquisition module II is used for acquiring actual pulse signals generated by blood cells passing through the gem hole and sending the actual pulse signals to the storage module II;
the identification module is used for identifying a valid reference pulse signal in the first storage module;
the calculation module is used for calculating a calibration coefficient according to the ratio of the sampling data of the rising edge and the falling edge of the effective reference pulse signal;
and the calibration module is used for re-fitting the pulse waveform of the actual pulse signal according to the calibration coefficient.
It should be understood that this embodiment is a system example corresponding to the first embodiment, and may be implemented in cooperation with the first embodiment. The related technical details mentioned in the first embodiment are still valid in this embodiment, and are not described herein again in order to reduce repetition. Accordingly, the related-art details mentioned in the present embodiment can also be applied to the first embodiment.
It should be noted that each module referred to in this embodiment is a logical module, and in practical applications, one logical unit may be one physical unit, may be a part of one physical unit, and may be implemented by a combination of multiple physical units. In addition, in order to highlight the innovative part of the present invention, elements that are not so closely related to solving the technical problems proposed by the present invention are not introduced in the present embodiment, but this does not indicate that other elements are not present in the present embodiment.
The third embodiment:
as shown in fig. 6, a third embodiment of the present invention provides a network side server, including: at least one processor 301; and a memory 302 communicatively coupled to the at least one processor; wherein the memory 302 stores instructions executable by the at least one processor 301 to enable the at least one processor 301 to perform one of the above-described blood cell pulse count error correction methods.
Where the memory 302 and the processor 301 are coupled in a bus, the bus may comprise any number of interconnected buses and bridges, the buses coupling one or more of the various circuits of the processor 301 and the memory 302. The bus may also connect various other circuits such as peripherals, voltage regulators, power management circuits, and the like, which are well known in the art, and therefore, will not be described any further herein. A bus interface provides an interface between the bus and the transceiver. The transceiver may be one element or a plurality of elements, such as a plurality of receivers and transmitters, providing a means for communicating with various other apparatus over a transmission medium. The data processed by the processor 301 is transmitted over a wireless medium through an antenna, which further receives the data and transmits the data to the processor 301.
The processor 301 is responsible for managing the bus and general processing and may also provide various functions including timing, peripheral interfaces, voltage regulation, power management, and other control functions. And memory 302 may be used to store data used by processor 301 in performing operations.
The foregoing is merely an example of the present invention, and common general knowledge in the field of known specific structures and characteristics is not described herein in any greater extent than that known in the art at the filing date or prior to the priority date of the application, so that those skilled in the art can now appreciate that all of the above-described techniques in this field and have the ability to apply routine experimentation before this date can be combined with one or more of the present teachings to complete and implement the present invention, and that certain typical known structures or known methods do not pose any impediments to the implementation of the present invention by those skilled in the art. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims (4)

1. A blood cell pulse counting error correction method is characterized by comprising the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
collecting reference pulse signals generated when a plurality of standard particles continuously pass through a gem hole under a constant pressure state;
storing the reference pulse signal according to the acquisition time, extracting the pulse waveform of the reference pulse signal, and judging whether the reference pulse signal is effective;
extracting effective reference pulse signal, calibrating error of gem hole, and calculating calibration coefficient
Figure 663616DEST_PATH_IMAGE001
；
Arranging the effective reference pulse signals according to the collection time sequence and marking
Figure 111915DEST_PATH_IMAGE002
，
Figure 94915DEST_PATH_IMAGE003
Representing the number of valid reference pulse signals;
calculating data from rising edge to falling edge of each effective reference pulse signal
Figure 145916DEST_PATH_IMAGE004
；
Calculating calibration coefficients
Figure 225868DEST_PATH_IMAGE001
The specific calculation is as follows,
Figure 454855DEST_PATH_IMAGE005
Figure 253047DEST_PATH_IMAGE006
…
Figure 901547DEST_PATH_IMAGE007
Figure 976950DEST_PATH_IMAGE008
Figure 501473DEST_PATH_IMAGE009
；
collecting actual pulse signals of blood cells passing through the gem hole, and extracting pulse waveforms of the actual pulse signals;
using calibration coefficients
Figure 646015DEST_PATH_IMAGE001
Refitting the falling edge pulse waveform of the actual pulse signal;
acquiring sampling data of actual pulse signal
Figure 914185DEST_PATH_IMAGE010
；
Acquiring sampling data of actual pulse signal falling edge from peak value to end point
Figure 640833DEST_PATH_IMAGE011
；
Using calibration coefficients
Figure 726469DEST_PATH_IMAGE001
Calibrating the sampling data of the actual pulse signal to obtain the corrected sampling data of the pulse signal
Figure 233674DEST_PATH_IMAGE012
And the specific calibration process is as follows,
Figure DEST_PATH_IMAGE013
Figure 367852DEST_PATH_IMAGE014
Figure 138887DEST_PATH_IMAGE015
…
Figure 739632DEST_PATH_IMAGE016
Figure 875078DEST_PATH_IMAGE018
Figure 485051DEST_PATH_IMAGE019
…
Figure 435559DEST_PATH_IMAGE020
Figure 348151DEST_PATH_IMAGE021
Figure 829948DEST_PATH_IMAGE022
。
2. the method according to claim 1, wherein the method further comprises: the standard particles are latex spherical substances.
3. The method according to claim 2, wherein the step of storing the reference pulse signal at the sampling time, the step of extracting the pulse waveform of the reference pulse signal, and the step of determining whether or not the reference pulse signal is valid specifically comprises:
acquiring the maximum value of a sampling signal corresponding to the rising edge of the pulse waveform;
setting the starting point of the rising edge of the pulse waveform according to 5% of the maximum value of the sampling signal
Figure 368246DEST_PATH_IMAGE023
And the end point of the falling edge
Figure 251888DEST_PATH_IMAGE024
Selecting a starting point
Figure 397699DEST_PATH_IMAGE023
And a termination point
Figure 225846DEST_PATH_IMAGE024
The pulse waveform in between as the detection waveform;
judging whether the reference pulse signal is an effective pulse signal according to the detected waveform, and determining whether the reference pulse signal is an effective pulse signal when the start point of the detected waveform
Figure 177622DEST_PATH_IMAGE023
End point of
Figure 56716DEST_PATH_IMAGE024
And pulse width
Figure 955703DEST_PATH_IMAGE025
All the pulse waveforms are in accordance with the judgment condition, and the pulse waveforms are judged to be effective reference pulse signals,
Figure 412092DEST_PATH_IMAGE026
wherein:
Figure 777346DEST_PATH_IMAGE023
indicating the starting point of the rising edge of the pulse waveform,
Figure 370001DEST_PATH_IMAGE024
indicating the end point of the falling edge of the pulse waveform,
Figure 106882DEST_PATH_IMAGE025
which represents the pulse width of the pulse waveform,
Figure 925933DEST_PATH_IMAGE027
representing the maximum value of the sampled signal.
4. A blood cell pulse count error correction apparatus according to the method of claim 1, wherein: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
the acquisition module I is used for acquiring a reference pulse signal generated by standard particles passing through a gem hole and sending the reference pulse signal to the storage module I;
the acquisition module II is used for acquiring actual pulse signals generated by blood cells passing through the gem hole and sending the actual pulse signals to the storage module II;
the identification module is used for identifying a valid reference pulse signal in the first storage module;
the calculation module is used for calculating a calibration coefficient according to the ratio of the sampling data of the rising edge and the falling edge of the effective reference pulse signal;
and the calibration module is used for re-fitting the pulse waveform of the actual pulse signal according to the calibration coefficient.
CN202210392441.5A 2022-04-15 2022-04-15 Blood cell pulse counting error correction method and correction device Active CN114486689B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210392441.5A CN114486689B (en) 2022-04-15 2022-04-15 Blood cell pulse counting error correction method and correction device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210392441.5A CN114486689B (en) 2022-04-15 2022-04-15 Blood cell pulse counting error correction method and correction device

Publications (2)

Publication Number Publication Date
CN114486689A CN114486689A (en) 2022-05-13
CN114486689B true CN114486689B (en) 2022-07-05

Family

ID=81487819

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210392441.5A Active CN114486689B (en) 2022-04-15 2022-04-15 Blood cell pulse counting error correction method and correction device

Country Status (1)

Country Link
CN (1) CN114486689B (en)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR204610A1 (en) * 1972-03-27 1976-02-20 Coulter W APPARATUS FOR THE AUTOMATIC CORRECTION OF INACCURACIES IN THE COUNTING OF COINCIDENT PARTICLES IN A PARTICLE ANALYZER OF THE TYPE THAT HAS A SENSOR REGION
US3949198A (en) * 1972-03-27 1976-04-06 Coulter Electronics, Inc. Methods and apparatuses for correcting coincidence count inaccuracies in a coulter type of particle analyzer
JPH11223541A (en) * 1998-02-05 1999-08-17 Hitachi Ltd Calibration testing device for pulse doppler type ultrasonic flowmeter
US20070211829A1 (en) * 2001-10-22 2007-09-13 Matsushita Electric Industrial Co., Ltd. Method and apparatus for pulse optimization for non-linear filtering
CN106769698B (en) * 2016-12-29 2019-08-06 迪瑞医疗科技股份有限公司 A kind of haemocyte abnormal pulsers signal identification processing method based on theory of electrical impedance
JP6883248B2 (en) * 2018-03-26 2021-06-09 株式会社Ｊｖｃケンウッド Particle measuring machine, analyzer, and analysis method
CN109323975B (en) * 2018-11-07 2021-05-25 中国科学院合肥物质科学研究院 OPC (optical proximity correction) counting correction method based on echo threshold comparison
US11703563B2 (en) * 2020-09-24 2023-07-18 Integrated Device Technology, Inc. Sliding window and DC offset correction technique for pulse doppler radar systems

Also Published As

Publication number Publication date
CN114486689A (en) 2022-05-13

Similar Documents

Publication Publication Date Title
EP3833983B1 (en) Automatic calibration of laboratory instruments
CN102177427B (en) Detecting and handling coincidence in particle analysis
AU2019250160B2 (en) System and method for producing statistically valid assay means and ranges for quality control materials
CN104365024B (en) Determining the signal quality of an electrical interconnect
CN110401499A (en) For Fast Frequency Offset calibration method, device, equipment and the medium of radio-frequency module
US10902593B2 (en) Methods and apparatus for extended dynamic range from single exposures in capillary electrophoresis
CN106769698B (en) A kind of haemocyte abnormal pulsers signal identification processing method based on theory of electrical impedance
CN110987769B (en) Calibration method of liquid particle counter
CN116072305B (en) Clinical trial data acquisition system
CN107181300A (en) Charge protector, the method and apparatus for calibrating charging equipment
CN114486689B (en) Blood cell pulse counting error correction method and correction device
CN108931463B (en) Blood cell pulse recognition method and device based on sheath flow impedance principle
US10338085B2 (en) Devices and methods to determine whether to calibrate a laboratory analyzer
CN103743878A (en) Water quality monitoring data processing method and device
CN109996205A (en) Data Fusion of Sensor method, apparatus, electronic equipment and storage medium
CN105424916A (en) Electrode codes of biosensor test paper, recognition device and recognition method
CN109633243B (en) Beam signal peak amplitude accurate extraction method based on multiphase sampling
CN112305487A (en) Laboratory error calibration system and method for digital direct current electric energy meter
CN104535814A (en) Device and method for correcting high-speed sampling oscilloscope trigger jitter
CN112566170A (en) Network quality evaluation method, device, server and storage medium
CN109525308B (en) Optical module detection method, device, equipment and storage medium
CN106680756A (en) Testing method, system and device for accuracy grade of direct-current voltage transformer
CN106856417B (en) Method and device for detecting antenna feeder installation problem
CN215263989U (en) Calibration system of battery module detection equipment
WO2017160857A1 (en) System and method for ensuring quality control of a plurality of laboratory instruments

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant