CN114479117B - Bioactive hydrogel supporting suspended 3D printing and application method thereof - Google Patents

Bioactive hydrogel supporting suspended 3D printing and application method thereof Download PDF

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CN114479117B
CN114479117B CN202011271726.0A CN202011271726A CN114479117B CN 114479117 B CN114479117 B CN 114479117B CN 202011271726 A CN202011271726 A CN 202011271726A CN 114479117 B CN114479117 B CN 114479117B
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cells
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CN114479117A (en
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刘纯
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First Affiliated Hospital of Sun Yat Sen University
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Abstract

The invention provides a hydrogel with bioactivity for supporting suspended 3D printing and an application method thereof, wherein the hydrogel contains the following components in concentration: type I collagen of more than 0.5 to less than or equal to 10mg/ml, hyaluronic acid of more than 0.1 to less than or equal to 10mg/ml, sodium alginate of more than 0.1 to less than or equal to 50mg/ml, polyethylene glycol cage polysilsesquioxane of more than 0.1 to less than or equal to 500 ul/ml. The hydrogel material can support suspension 3D printing and cell growth, can realize high-precision 3D cell printing, and ensures that cells in the biological ink can keep cell activity in suspension gel, thereby realizing technical innovation for reconstructing tissues and organs in 3D printing.

Description

Bioactive hydrogel supporting suspended 3D printing and application method thereof
Technical Field
The invention belongs to the technical field of 3D biological printing, and particularly relates to the technical field of hydrogel materials for 3D biological printing.
Background
In recent years, the development of the fields of regenerative medicine and tissue engineering brings about innovative technology and platform for constructing in-vitro bionic tissue organ models. However, most in-vitro 3D models are still limited to the traditional tissue engineering thought of a cell-scaffold, and the distance simulation of real tissues and organs still has a great gap, so that the design accuracy and the dimension are difficult to break through. The 3D biological printing technology provides a future development direction for solving the bottleneck of scientific research. The cell 3D printing is to take living cells as basic construction units, assist in taking biological materials as printing ink, place substances such as cells/biological materials/growth factors and the like at specific space positions through a 3D printing technology according to a pre-designed computer model under the guidance of a bionic principle and a developmental biological principle, and form a required three-dimensional structure body through layer-by-layer bonding. The structure can be cultured in vitro for a long time, and cells can be proliferated and differentiated in a three-dimensional environment under the proper culture solution and fluid conditions. With the gradual growth, development and maturation of artificial tissues, it is possible to form artificial tissues and organs having physiological functions. By designing the bionic printing ink and the organ tissue structure, the 3D biological printing can take cells blended into the matrix ink as basic units for reconstructing tissues, so that the double bionics on the structure and the components of the real tissue organ is achieved.
Nowadays, cell 3D printing technology related research presents a explosive growth trend. At present, the cell 3D printing technology is used for constructing tissues such as cartilage, skin, muscle, blood vessel, cardiac muscle, lung, nerve and the like of a human body, however, at present, the 3D living cell biological printing is still in a primary development stage, and precise regulation and control on single cell and microenvironment components are still difficult to realize. The specific technical difficulties are shown in the following aspects: 1) The printing ink material has great limitation, the mechanical property of the biological ink is poor, and good structural support is difficult to achieve; 2) The high molecular ink has good mechanical property, but poor biological activity, and can not support healthy growth of cells; 3) The printing precision is still limited to hundred micrometers, and higher and even single-cell precision is difficult to realize, so that precise control of fine structures inside tissues and organs is difficult; 4) On the subsequent culture after printing, the cells are difficult to break through the interface of the printing material and can only be limited in silk to influence the movement and development of the cells, so that ideal biological functions are difficult to achieve.
Therefore, the latest research innovates the technology of living cell 3D printing by a suspension 3D printing mode, and improves the precision of living cell printing to a new height. The suspension printing uses the hydrogel material which is thinned by shearing as a suspension adhesive supporting bath, and the living cell ink is directly printed into the suspension adhesive, so that the buoyancy supporting printing structure provided by the suspension adhesive is realized, the thinner extrusion wire diameter is realized, the printing precision is improved, and the guarantee is provided for printing complex and fine internal structures. Representative of the outcome is the beating heart of the suspension printing published in Science journal in 2019.
However, although suspension printing offers a completely new solution in concept for 3D live cell printing, it is still limited to materials in practical applications. At present, the materials which can be provided for suspension printing are mainly carbomer gel high molecular polymers, and the shear thinning and self-healing effects are realized through hydrogen bonds, so that the extruded biological ink can stay in place during printing. However, the biggest problem with such materials is that they do not support the growth of cells. The suspension must therefore be washed away after printing is completed to ensure the cell viability of the printing. The process of cleaning the suspension is a process of removing the supporting buoyancy, so that many fine structures still have difficulty achieving self-support and collapse. Therefore, the current suspension 3D printing technology is urgently needed to solve the problem of developing a suspension gel material which can support cell activity and does not need to be washed after printing is completed.
Disclosure of Invention
The invention aims to make up the defects of the prior art and provides a suspension gel material which supports cell activity and does not need to be washed after printing.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a bioactive hydrogel supporting suspended 3D printing, the formulation comprising the following components:
in particular, the type I collagen comprises a mixture of one or both of murine and human origin.
Preferably, the hyaluronic acid is selected from animal origin, or microbial origin or human origin, and has a molecular weight of 1000-300000.
Specifically, in the molecular structure of the polyethylene glycol cage polysilsesquioxane, polyethylene glycol groups may be present on one to eight silicon atom-linked side chains of the silicon cage structure.
A second object of the present invention is to provide a 3D suspension printing method suitable for living cells.
In order to achieve the above purpose, the present invention adopts the following technical scheme: a 3D suspension printing method using the hydrogel of any one of the above, comprising the steps of:
(1) Preparing suspension gel
Under the aseptic condition, mixing type I collagen, hyaluronic acid and sodium alginate until uniform, adding polyethylene glycol cage polysilsesquioxane, and uniformly mixing again to prepare suspension gel;
(2) Preparation of living cell biological ink
Mixing the cells to be printed with 1% -10% of methylacrylate gelatin to obtain living cell biological ink;
(3) 3D printing and curing suspension adhesive
Placing a needle head of an injector filled with biological ink into a printing spray head for precooling, heating to 18 ℃, inserting the printing needle head into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, dripping calcium chloride on the surface of the suspension adhesive after printing, placing the suspension adhesive and a culture dish into an incubator, and solidifying the suspension adhesive; or alternatively, the process may be performed,
adding 3.5% of methacrylate gelatin accounting for 30% of the total volume into the suspension adhesive obtained in the step (1) before printing, placing a syringe needle filled with biological ink into a printing nozzle for precooling, heating to 18 ℃, inserting the printing needle into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, and simultaneously curing the suspension adhesive and printing ink in a photocrosslinking mode after printing is finished.
Preferably, the substitution rate of methacrylic acid in the methacrylate-treated gelatin is 20% -70%.
Preferably, the concentration of calcium chloride instilled in step (4) is matched according to the requirements in the tissue organ system in which the cells are to be printed.
Specifically, the cells to be printed include stem cells, myocardial cells, liver cells, breast cells, pancreatic cells, osteoblasts, chondrocytes, tracheal epithelial progenitor cells, vascular endothelial cells, nerve cells, and tumor cells.
The third object of the invention is to provide a method for 3D suspension printing and culturing of living cells.
In order to achieve the above purpose, the present invention adopts the following technical scheme: a method for 3D suspension printing and culturing of living cells using a hydrogel as described in any one of the preceding claims, comprising the steps of:
(1) Preparing suspension gel
Under the aseptic condition, mixing type I collagen, hyaluronic acid and sodium alginate until uniform, adding polyethylene glycol cage polysilsesquioxane, and uniformly mixing again to prepare suspension gel;
(2) Preparation of living cell biological ink
Mixing stem cells with 10% of methacrylate gelatin to obtain living cell bio-ink;
(3) 3D printing and curing suspension adhesive
Placing a needle head of an injector filled with biological ink into a printing spray head for precooling, heating to 18 ℃, inserting the printing needle head into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, dripping calcium chloride on the surface of the suspension adhesive after printing, placing the suspension adhesive and a culture dish into an incubator, and solidifying the suspension adhesive; or alternatively, the process may be performed,
adding 3.5% of methacrylate gelatin accounting for 30% of the total volume into the suspension adhesive obtained in the step (1) before printing, placing a syringe needle filled with biological ink into a printing nozzle for precooling, heating to 18 ℃, inserting the printing needle into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, and simultaneously curing the suspension adhesive and printing ink in a photocrosslinking mode after printing is finished.
(4) Cell culture
And (3) dropwise adding a cell culture medium matched with the cells to be printed on the surface of the suspension gel after 1 hour, and continuously putting the cells back into the incubator for culture.
The hydrogel of the invention provides the biological activity of the material through the type I collagen and the hyaluronic acid in the formula thereof, and supports the growth of cells; sodium alginate can provide material viscosity and integrity; PEG POSS is a mixed material system to increase self-healing function, wherein PEG side chain groups provide abundant hydrogen bonds in the material, so that the effect of shear thinning is achieved. In addition, the hardness in the material system is regulated by calcium chloride to match the actual hardness of each tissue and organ, so that the function of promoting the in-vitro growth and differentiation of cells is achieved.
The invention can be applied to biological 3D printing of various tissues and organs, supports the growth and differentiation of cells in the suspension gel, realizes the reconstruction of tissues and organs in vitro, can be applied to the modeling and in vitro reconstruction of tissues and organs such as heart, liver, mammary gland, pancreas, bone, cartilage, muscle, trachea, blood vessels, cornea, nerve, tumor and the like, and can also be used for stem cell differentiation to manufacture in vitro organoid models.
The in vitro tissues and organs printed by the suspension method can be applied to various basic researches including organ responses, intracellular molecular biological signals, pathological researches serving as models of diseases and normal organs, in vitro training models serving as drug screening models and various clinical intervention means (interventional therapy, radiotherapy and the like).
The invention can provide good support performance for living cell biological 3D printing, so that the printing precision is improved, finer wire diameter (less than 100 microns) and more complicated and fine cavity structure printing can be realized, meanwhile, the suspension gel provides good in-vitro microenvironment for cells, the activity of the cells can be ensured, and the original functions of dryness, proliferation, metabolism and the like are maintained.
The hydrogel material can support suspension 3D printing and cell growth, can realize high-precision 3D cell printing, and ensures that cells in the biological ink can keep cell activity in suspension gel, thereby realizing technical innovation for reconstructing tissues and organs in 3D printing.
Drawings
FIG. 1 is a 3d structure image formed by 3d printing of a collagen-containing bio-ink in a suspension gel of the present invention, (a) is a schematic diagram of printing collagen in a suspension gel using a 32G needle; (b) Is a schematic of printing a bio-ink containing cells in a suspension gel.
FIG. 2 is a photograph of tumor cells (brain glioma cells) printed in 3D in the suspension gel of the invention, (a) the first day the cells were cultured after suspension printing, and (b) the second day the cells were cultured after suspension printing.
In the figure, 1-cell-containing bio-ink, 2-suspension gel
Detailed Description
Example 1
This example is a 3D printing and culture example of spinal mesenchymal stem cells.
1. Preparation of suspension gel
Under the aseptic condition, the reagent type I collagen (selected from the Germany Ibidi brand), hyaluronic acid (Sigma) and sodium alginate (Sigma) are proportionally measured and mixed until uniform, then PEG POSS (Hybrid plastic) is added and mixed uniformly again to prepare the suspension gel, so that the final concentration of the suspension gel is 3mg/ml of the type I collagen, 0.1mg/ml of the hyaluronic acid, 5mg/ml of the sodium alginate and 0.05% of the PEG POSS by mass.
The type I collagen in this example is selected from the group consisting of type I collagen of murine origin, ibidi, germany.
The hyaluronic acid is selected from Sigma human source, and has a molecular weight of 1000-300000. The hyaluronic acid may be optionally thiol-modified or unmodified.
The sodium alginate includes Sigma with low viscosity, medium and high viscosity, or a mixture of both.
In the molecular structure of the polyethylene glycol cage polysilsesquioxane (PEG POSS), polyethylene glycol (PEG) groups can exist on one to eight silicon atom connecting side chains of the POSS silicon cage structure.
2. Preparation of living cell bio-ink
5x 10-6/ml mouse spinal cord mesenchymal stem cells (other stem cells such as neural stem cells, or myocardial cells, liver cells, breast cells, pancreatic cells, osteoblasts, chondrocytes, tracheal epithelial progenitor cells, vascular endothelial cells, nerve cells and the like) are mixed with 5ml 10% methylacrylate gelatin to obtain the living cell bio-ink with the final cell concentration of 1x 10-6 cells/ml.
3.3D printing and curing suspension adhesive
The 3D printing is carried out by adopting a hydrogel extrusion mode, the spray head is precooled to 4 degrees, and the temperature of a printing platform is set to 23 degrees.
And (5) placing the 32G needle head of the injector filled with the biological ink into a printing spray head for precooling. And placing the prepared suspension adhesive into a cell culture dish, and placing the cell culture dish on a printing platform.
After five minutes, the temperature of the printing nozzle is raised to 18 ℃, and after the printing nozzle is extruded into a smooth thread shape, the printing needle head is inserted into the suspension adhesive to start printing. The printing speed is 5mm/s, and the extrusion speed is 0.15mm 3 /s。
Synchronous blue crosslinking is performed during printing.
Sterility in the printer is ensured during printing.
And after printing, the printing head is retracted, 7.5mg/ml of calcium chloride is dripped on the surface of the suspension adhesive until the printing structure is fully soaked, the suspension adhesive and the culture dish are put into an incubator, and the suspension adhesive is solidified. 4. Cell culture
After 1 hour, cell culture medium is dripped on the surface of the suspension gel and is continuously put back into the incubator for culture. The cell culture medium is a conventional medium of spinal mesenchymal stem cells.
The cells were cultured on the second day after suspension printing without apparent apoptosis, without a decrease in cell number and with a larger morphology than on the first day.
After 3-7 days of culture, the cells can be subjected to immunohistochemical staining, differentiation and proliferation conditions of the mesenchymal stem cells are checked, and the mesenchymal stem cells can be verified to proliferate and differentiate normally after printing.
At different time points in the culture, the suspension gel can be dissolved by adopting sodium citrate and type I collagenase, cellular RNA is extracted for qPCR or RNA sequencing, and the transcriptomic difference of the cells before and after printing is verified.
When the invention prepares the suspension adhesive, the following components can be added in the step 1: antibacterial polypeptides, inflammatory factors, fibroblasts and other mesenchymal cells (stroma), immune cells, various growth factors, cytokines, cell culture media, serum proteins, acellular matrix, etc.
Gelatin materials can be added in the formula to improve the mechanical strength.
Other groups such as amino acid, polypeptide, antibody and the like can be grafted on the silicon cage structure of the PEG POSS in the formula through chemical modification.
The formula is suitable for 3D living cell printing with a single spray head or multiple spray heads.
The 3D printed in-vitro tissue or organ manufactured by the formula can be used for in-vivo transplantation after in-vitro culture for a period of time.
The formulation material also supports cell-free bio-ink 3D printing.
Example 2
This example is a suspended 3D printing and culture example of brain glioma.
1. Preparation of suspension gel
Under the aseptic condition, 3.67mg/m l type I collagen, 10mg/ml hyaluronic acid (Sigma) and 10mg/ml sodium alginate (Sigma) are mixed to be uniform, 100ul/ml PEG POSS (Hybrid plastic) is added, and the mixture is uniformly mixed again to prepare the suspension adhesive, wherein the final concentration of each component in the prepared suspension adhesive is 1.5mg/ml of type I collagen, 0.5mg/ml hyaluronic acid, 3mg/ml sodium alginate and 0.1% of PEG POSS mass percentage concentration.
The type I glue is selected from BD murine type I collagen in the United states.
The hyaluronic acid is selected from Sigma human source, and has a molecular weight of 1000-300000. The hyaluronic acid may be optionally thiol-modified or unmodified.
The sodium alginate comprises Sigma with low viscosity, and one or both of low viscosity and medium and high viscosity can be used.
In the molecular structure of the polyethylene glycol cage polysilsesquioxane (PEG POSS), polyethylene glycol (PEG) groups can exist on one to eight silicon atom connecting side chains of the POSS silicon cage structure.
2. Preparation of living cell bio-ink
10x 10-6/ml human brain glioma cells (or tumor stem cells, or tumor cells such as breast cancer, prostatic cancer, liver cancer, cholangiocarcinoma, lung cancer, oral squamous carcinoma, esophageal cancer and the like) are mixed with 5ml gelatin sodium alginate (the ratio of 30% gelatin to 10% sodium alginate is 1:1), so that the living cell bio-ink with the final cell concentration of 2x 10-6 cells/ml is obtained.
3.3D printing and curing suspension adhesive
And 3D printing is performed by adopting a hydrogel extrusion mode, the spray head is precooled to 4 degrees, and the temperature of a printing platform is set to 18 degrees.
The syringe filled with the biological ink is provided with a 28G needle head and is placed into a printing nozzle for precooling. And placing the prepared suspension adhesive into a cell culture dish, and placing the cell culture dish on a printing platform.
After five minutes, the temperature of the printing nozzle is raised to 22 ℃, after the printing nozzle is extruded into smooth thread, the printing needle head is inserted into the suspension adhesive to start printing (the printing speed is 5mm/s, and the extrusion speed is 0.15 mm) 3 /s)。
Sterility in the printer is ensured during printing.
And after printing, the printing head is retracted, 30mg/ml of calcium chloride is dripped on the surface of the suspension adhesive until the printing structure is fully soaked, the suspension adhesive and the culture dish are put into an incubator, and the suspension adhesive is solidified.
4. Cell culture
After 1 hour, cell culture medium is dripped on the surface of the suspension gel and is continuously put back into the incubator for culture.
After 3-7 days of culture, the cells can be subjected to immunohistochemical staining, differentiation and proliferation conditions of the mesenchymal stem cells are checked, and the mesenchymal stem cells can be verified to proliferate and differentiate normally after printing.
At different time points in the culture, the suspension gel can be dissolved by adopting sodium citrate and type I collagenase, cellular RNA is extracted for qPCR or RNA sequencing, and the transcriptomic difference of the cells before and after printing is verified.
The formula can also be added with the following components: antibacterial polypeptides, inflammatory factors, fibroblasts and other mesenchymal cells (stroma), immune cells, various growth factors, cytokines, cell culture media, serum proteins, acellular matrix, etc.
Gelatin materials can be added in the formula to improve the mechanical strength.
Other groups such as amino acid, polypeptide, antibody and the like can be grafted on the silicon cage structure of the PEG POSS in the formula through chemical modification.
The formula is suitable for 3D living cell printing with a single spray head or multiple spray heads.
The 3D printed in-vitro tissue or organ manufactured by the formula can be used for in-vivo transplantation after in-vitro culture for a period of time.
The formulation material also supports cell-free bio-ink 3D printing.
Example 3
This example is a 3D printing and culture example of osteoblasts.
1. Preparation of suspension gel
Under the aseptic condition, the reagent type I collagen (selected from the Germany Ibidi brand), hyaluronic acid (Sigma) and sodium alginate (Sigma) are proportionally measured and mixed until uniform, then PEG POSS (Hybrid plastic) is added and mixed uniformly again to prepare the suspension gel, so that the final concentration of the suspension gel is 3mg/ml of the type I collagen, 0.1mg/ml of the hyaluronic acid, 5mg/ml of the sodium alginate and 0.05% of the PEG POSS by mass.
The type I collagen in this example is selected from the group consisting of type I collagen of murine origin, ibidi, germany.
The hyaluronic acid is selected from Sigma human source, and has a molecular weight of 1000-300000. The hyaluronic acid may be optionally thiol-modified or unmodified.
The sodium alginate includes Sigma with low viscosity, medium and high viscosity, or a mixture of both.
In the molecular structure of the polyethylene glycol cage polysilsesquioxane (PEG POSS), polyethylene glycol (PEG) groups can exist on one to eight silicon atom connecting side chains of the POSS silicon cage structure.
2. Preparation of living cell bio-ink
Uniformly mixing 8x 10-6/ml mouse osteoblasts MC4 and 5ml gelatin sodium alginate mixed gel containing hydroxyapatite nanoparticles, wherein the gelatin concentration is 10%, the sodium alginate concentration is 5%, and the concentration of the hydroxyapatite nanoparticles is 0.1%, so as to obtain the living cell biological ink with the final cell concentration of 1x 10-6 cells/ml.
The gelatin is selected from Sigma, is derived from pig skin, and is derived from bovine skin or human skin.
The hydroxyapatite nanoparticle is selected from Sigma, the particle size is less than 200 nanometers, and micron particles with the particle size of 5 microns can also be used.
3.3D printing and curing suspension adhesive
The suspension gel obtained in step (1) before printing was mixed with a concentration of 3.5% of methacrylated gelatin (GelMA) to make it a final GelMA of 30% of the total volume.
Placing the needle head of the injector filled with the biological ink into a printing spray head for precooling, heating to 20 ℃, inserting the printing needle head into the suspension adhesive, and starting printing at a printing speed of 5mm/s and an extrusion speed of 0.15mm 3 And/s. After printing, the suspension was cured by blue light irradiation for 20 seconds.
4. Cell culture
After 1 hour, cell culture medium is dripped on the surface of the suspension gel and is continuously put back into the incubator for culture. The cell culture medium is a conventional medium for osteoblasts.
The following day of cell culture after suspension printing showed no apparent apoptosis, no drop in cell number and no change in morphology from the first day.
After 3-7 days of culture, the cells can be induced into bone differentiation in the same way as the conventional 2D culture, and the bone differentiation can be characterized after 7-14 days of induced differentiation, including the conventional ways of immunohistochemical staining and the like.
At different time points in the culture, the suspension gel can be dissolved by adopting sodium citrate and type I collagenase, cellular RNA is extracted for qPCR or RNA sequencing, and the transcriptomic difference of the cells before and after printing is verified.
When the invention prepares the suspension adhesive, the following components can be added in the step 1: antibacterial polypeptides, inflammatory factors, fibroblasts and other mesenchymal cells (stroma), immune cells, various growth factors, cytokines, cell culture media, serum proteins, acellular matrix, etc.
Other groups such as amino acid, polypeptide, antibody and the like can be grafted on the silicon cage structure of the PEG POSS in the formula through chemical modification.
The formula is suitable for 3D living cell printing with a single spray head or multiple spray heads.
The 3D printed in-vitro tissue or organ manufactured by the formula can be used for in-vivo transplantation after in-vitro culture for a period of time.
The formulation material also supports cell-free bio-ink 3D printing.
The above embodiments are only for illustrating the present invention, not for limiting the present invention, and various changes and modifications may be made by one of ordinary skill in the relevant art without departing from the spirit and scope of the present invention, and therefore, all equivalent technical solutions are also within the scope of the present invention, and the scope of the present invention is defined by the claims.

Claims (9)

1. A bioactive hydrogel supporting suspended 3D printing, characterized in that the formulation comprises the following concentrations of components: type I collagen comprising one or a mixture of murine and human sources at a concentration of 3mg/ml, an
Hyaluronic acid, selected from animal origin, or microbial origin or human origin, having a molecular weight of 1000-300000 and a concentration of 0.1mg/ml, and
sodium alginate at a concentration of 5mg/ml, and
the mass percentage of polyethylene glycol cage type polysilsesquioxane is 0.05%.
2. A bioactive hydrogel supporting suspended 3D printing, characterized in that the formulation comprises the following concentrations of components: type I collagen comprising one or a mixture of murine and human sources at a concentration of 1.5mg/ml, and
hyaluronic acid, selected from animal origin, or microbial origin or human origin, having a molecular weight of 1000-300000 and a concentration of 0.5mg/ml, and
sodium alginate at a concentration of 3mg/ml, and
polyethylene glycol cage type polysilsesquioxane with the mass percentage concentration of 0.1 percent.
3. A bioactive hydrogel supporting suspended 3D printing, characterized in that the formulation comprises the following concentrations of components: type I collagen comprising one or a mixture of murine and human sources at a concentration of 3mg/ml, an
Hyaluronic acid, selected from animal origin, or microbial origin or human origin, having a molecular weight of 1000-300000 and a concentration of 0.1mg/ml, and
sodium alginate at a concentration of 5mg/ml, and
polyethylene glycol cage type polysilsesquioxane, the mass percentage of which is 0.05%.
4. The bioactive hydrogel supporting suspended 3D printing of claim 1, wherein: in the molecular structure of the polyethylene glycol cage type polysilsesquioxane, polyethylene glycol groups can exist on one to eight silicon atom connecting side chains of the silicon cage structure.
5. A 3D suspension printing method using the hydrogel according to any one of claims 1 to 4, characterized by comprising the steps of:
(1) Preparing suspension gel
Under the aseptic condition, mixing type I collagen, hyaluronic acid and sodium alginate until uniform, adding polyethylene glycol cage polysilsesquioxane, and uniformly mixing again to prepare suspension gel;
(2) Preparation of living cell biological ink
Mixing the cells to be printed with 1% -10% of methylacrylate gelatin to obtain living cell biological ink;
(3) 3D printing and curing suspension adhesive
Placing a needle head of an injector filled with biological ink into a printing spray head for precooling, heating to 18 ℃, inserting the printing needle head into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, dripping calcium chloride on the surface of the suspension adhesive after printing, placing the suspension adhesive and a culture dish into an incubator, and solidifying the suspension adhesive; or alternatively, the process may be performed,
adding 3.5% of methacrylate gelatin accounting for 30% of the total volume into the suspension adhesive obtained in the step (1) before printing, placing a syringe needle filled with biological ink into a printing nozzle for precooling, heating to 18 ℃, inserting the printing needle into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, and simultaneously curing the suspension adhesive and printing ink in a photocrosslinking mode after printing is finished.
6. The method according to claim 5, wherein: the substitution rate of the methacrylic acid in the methacrylate gelatin is 20-70%.
7. The method according to claim 5, wherein: the concentration of the calcium chloride dripped in the step (4) is matched according to the requirements in the tissue organ system where the cells are to be printed.
8. The method according to claim 5, wherein: the cells to be printed comprise stem cells, or myocardial cells, or liver cells, or breast cells, or pancreatic cells, or osteoblasts, or chondrocytes, or tracheal epithelial progenitor cells, or vascular endothelial cells, or nerve cells, or tumor cells.
9. A method for 3D suspension printing and culturing living cells using the hydrogel according to any one of claims 1 to 4, comprising the steps of:
(1) Preparing suspension gel
Under the aseptic condition, mixing type I collagen, hyaluronic acid and sodium alginate until uniform, adding polyethylene glycol cage polysilsesquioxane, and uniformly mixing again to prepare suspension gel;
(2) Preparation of living cell biological ink
Mixing stem cells with 10% of methacrylate gelatin to obtain living cell bio-ink;
(3) 3D printing and curing suspension adhesive
Placing a needle head of an injector filled with biological ink into a printing spray head for precooling, heating to 18 ℃, inserting the printing needle head into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, dripping calcium chloride on the surface of the suspension adhesive after printing, placing the suspension adhesive and a culture dish into an incubator, and solidifying the suspension adhesive; or alternatively, the process may be performed,
adding 3.5% of methacrylate gelatin accounting for 30% of the total volume into the suspension adhesive obtained in the step (1) before printing, placing a syringe needle filled with biological ink into a printing nozzle for precooling, heating to 18 ℃, inserting the printing needle into the suspension adhesive, starting printing, performing synchronous photocrosslinking in the printing process, and simultaneously curing the suspension adhesive and printing ink in a photocrosslinking mode after printing is finished;
(4) Cell culture
And (3) dropwise adding a cell culture medium matched with the cells to be printed on the surface of the suspension gel after 1 hour, and continuously putting the cells back into the incubator for culture.
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CN108567993A (en) * 2018-03-21 2018-09-25 暨南大学 A method of hypoglycemic artificial intelligence pancreas is used for based on 3D printing structure
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