CN114476330A - Soil storage and freeze-drying sample bottle for microbial detection and detection method thereof - Google Patents
Soil storage and freeze-drying sample bottle for microbial detection and detection method thereof Download PDFInfo
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- CN114476330A CN114476330A CN202210186032.XA CN202210186032A CN114476330A CN 114476330 A CN114476330 A CN 114476330A CN 202210186032 A CN202210186032 A CN 202210186032A CN 114476330 A CN114476330 A CN 114476330A
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- 238000004108 freeze drying Methods 0.000 title claims abstract description 33
- 239000002689 soil Substances 0.000 title claims abstract description 32
- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 238000012473 microbial detection method Methods 0.000 title description 2
- 238000005192 partition Methods 0.000 claims abstract description 16
- 244000005700 microbiome Species 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 13
- 230000000087 stabilizing effect Effects 0.000 claims description 10
- 230000002906 microbiologic effect Effects 0.000 claims description 7
- 230000008878 coupling Effects 0.000 claims description 6
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 abstract description 6
- 230000000813 microbial effect Effects 0.000 abstract description 3
- -1 Phospholipid fatty acid Chemical class 0.000 description 6
- 238000007789 sealing Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 5
- 239000004810 polytetrafluoroethylene Substances 0.000 description 5
- 239000005388 borosilicate glass Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 238000002955 isolation Methods 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/04—Partitions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D21/00—Nestable, stackable or joinable containers; Containers of variable capacity
- B65D21/02—Containers specially shaped, or provided with fittings or attachments, to facilitate nesting, stacking, or joining together
- B65D21/0209—Containers specially shaped, or provided with fittings or attachments, to facilitate nesting, stacking, or joining together stackable or joined together one-upon-the-other in the upright or upside-down position
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D23/00—Details of bottles or jars not otherwise provided for
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D51/00—Closures not otherwise provided for
- B65D51/16—Closures not otherwise provided for with means for venting air or gas
- B65D51/1672—Closures not otherwise provided for with means for venting air or gas whereby venting occurs by manual actuation of the closure or other element
- B65D51/1683—Closures not otherwise provided for with means for venting air or gas whereby venting occurs by manual actuation of the closure or other element by actuating a separate element in the container or closure
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A soil storage and freeze-drying sample bottle for microbial detection and a detection method thereof relate to the technical field of soil sample pretreatment. The invention aims to solve the problems that soil powder is easy to be pumped out by a vacuum pump in the conventional sample freeze-drying process, so that the sample is lost, and simultaneously, a cavity of a vacuum drier is polluted and even the vacuum pump is damaged. The sample storage bottle comprises a sample storage bottle, a partition plate, a bottle cap knob and two bottle cap inner embedded plates, wherein the partition plate is arranged in the sample storage bottle, the bottle cap is sleeved on the outer side of a bottle opening of the sample storage bottle, a group of bottle cap through holes are formed in the upper end face of the bottle cap, the middle of the upper end face of the bottle cap is rotatably connected with the bottle cap knob, a cover plate is fixedly connected onto the bottle cap knob and arranged on the outer side of the bottle cap through holes, the two bottle cap inner embedded plates are embedded into the inner side of the bottle cap in parallel, a group of inner embedded plate through holes are formed in one side of the inner embedded plate of the bottle cap, and the inner embedded plate through holes in the two bottle cap inner embedded plates are arranged in a staggered mode. The invention is used for soil microorganism detection.
Description
Technical Field
The invention relates to the technical field of soil sample pretreatment, in particular to a soil storage and freeze-drying sample bottle for microbial detection and a detection method thereof.
Background
Phospholipid fatty acid analysis (PLFA) is an important method for qualitative and quantitative determination of soil microorganisms, a soil sample for PLFA analysis needs to be stored at low temperature after being collected and is freeze-dried in a freeze dryer, the traditional soil sample is usually placed in a self-sealing bag after being collected, and the self-sealing bag is opened and is placed in the freeze dryer for freeze-drying after being returned to a laboratory. However, in the freeze-drying process, soil powder is easily pumped out by the vacuum freeze-drying machine, so that the cavity of the vacuum freeze-drying machine is polluted, samples are lost, and even the powder is pumped into the vacuum pump to damage the vacuum pump. In addition, in order to avoid introducing organic pollutants, the sample in the soil microorganism detection should be prevented from contacting plastic products except for polytetrafluoroethylene, so that a plastic self-sealing bag is avoided as much as possible.
Disclosure of Invention
The invention aims to quickly and efficiently finish the work of storing and freeze-drying soil samples so as to solve the problems that soil powder generated in the actual sample freeze-drying process is easy to be pumped out by a vacuum pump, so that the sample is lost, and simultaneously, a cavity of a vacuum drier is polluted, even the vacuum pump is damaged in the background art, and further provides a soil storage and freeze-drying sample bottle for microorganism detection and a detection method thereof.
The technical scheme adopted by the invention for solving the technical problems is as follows:
a sample bottle that is used for soil storage and freeze-drying of microbiological detection includes the sample storage bottle, the division board, the bottle lid, panel in bottle lid knob and two bottle lids, the uncovered setting of upper end bottleneck of sample storage bottle, the division board sets up and cuts apart into a plurality of storage area of equidimension in the sample storage bottle, the bottle lid suit is in the outside of sample storage bottle bottleneck, be equipped with a set of bottle lid through-hole on the up end of bottle lid, the middle part of bottle lid up end is rotated and is connected with the bottle lid knob, the rigid coupling has the apron on the bottle lid knob, the apron sets up the outside at the bottle lid through-hole, the inboard parallel of bottle lid is inlayed and is equipped with panel in two bottle lids, one side on the bottle lid inset panel is equipped with a set of inset panel through-hole, the crisscross setting of inset panel through-hole on two bottle lid inset panels.
Furthermore, the shape of sample storage bottle is cylindrical, and the bottleneck of sample storage bottle is the outward extension of arc curved surface.
Furthermore, the bottle cap is cylindrical, the lower end of the bottle cap is open, two annular grooves are formed in the inner circumferential side wall of the bottle cap in parallel, and the outer edge of an embedded plate in the bottle cap is arranged in the annular grooves.
Further, the bottle lid knob includes pivot and two plectrums, and the perpendicular rigid coupling of pivot is in the middle part of bottle lid up end, and two plectrums are the vertical setting of cross form, and the middle part suit of plectrum is in the pivot, and the apron rigid coupling is in the lower part between two plectrums.
Furthermore, the cover plate is in a quarter-sector shape, one side of the cover plate is fixedly connected with the lower end of one side of one shifting piece, and the other side of the cover plate is fixedly connected with the lower end of one side of the other shifting piece.
Furthermore, a set of bottle lid through-hole includes a plurality of bottle lid through-holes that are the equipartition of matrix form and set up, and a plurality of bottle lid through-holes use the pivot to set up along quarter fan-shaped region on the bottle lid.
Furthermore, the group of embedded plate through holes comprise a plurality of embedded plate through holes which are uniformly distributed in a matrix shape, and a plurality of embedded plate through holes are arranged in a square area on one side of the embedded plate.
Furthermore, the sample bottle for soil storage and freeze-drying for microorganism detection further comprises a stacking stabilizing frame which is fixedly connected to the outer side edge of the upper end face of the bottle cap.
Further, the stacking stabilizing frame comprises a plurality of limiting arc plates which are uniformly distributed along the circumferential direction of the bottle cap, and the lower ends of the limiting arc plates are fixedly connected to the outer side edge of the upper end face of the bottle cap.
The detection method of the soil storage and freeze-dried sample bottle for microorganism detection comprises the following steps:
the method comprises the following steps: firstly, placing a partition plate in a sample storage bottle, and then uniformly distributing collected soil samples into a plurality of areas partitioned by the partition plate in the sample storage bottle;
step two: installing two bottle cap inner embedded plates into a bottle cap, wherein the embedded plate through holes on the upper layer bottle cap embedded plate and the bottle cap through holes on the bottle cap are staggered at 180 degrees, and the embedded plate through holes on the lower layer bottle cap inner embedded plate and the embedded plate through holes on the upper layer bottle cap embedded plate are staggered at 180 degrees;
step three: rotating a bottle cap knob, rotating a cover plate to the outer side of a bottle cap through hole to enable the bottle cap through hole to be in a closed state, and then covering a bottle mouth of the sample storage bottle with a bottle cap;
step four: the method comprises the steps of putting sample storage bottles into a refrigerator with the temperature of-20 ℃ for pre-freezing, putting the sample storage bottles into a freeze dryer, rotating a bottle cap knob, keeping a cover plate away from a bottle cap through hole to enable the bottle cap through hole to be in an open state, stacking and placing a plurality of sample storage bottles in the height direction under the clamping of a stacking stabilizing frame, and starting the freeze dryer for freeze drying after the sample storage bottles are placed.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a sample bottle for soil storage and freeze-drying for microbial detection, which avoids sample pollution possibly caused by the contact of a sample with a plastic self-sealing bag, and simultaneously adopts air holes which are formed in a 180-degree crossed manner, so that the probability of pumping soil powder by a vacuum pump is reduced, the problem that the existing soil sample is easy to be pumped out by a vacuum freeze dryer to pollute the cavity of the vacuum freeze dryer in the freeze-drying process is solved, and the sample is prevented from polluting the cavity of the vacuum freeze dryer and damaging the vacuum pump.
The sample storage bottle is divided into a plurality of areas under the isolation of the partition plates so as to reduce the block volume of the soil sample and improve the freeze drying rate.
Meanwhile, the sample bottles can be stacked, the cavity space of the vacuum freeze dryer can be fully utilized, the number of freeze-dried samples in the same batch is increased, and the freeze-drying efficiency is improved.
Compared with the conventional valve bag freeze-drying, the soil sample storage and freeze-drying sample bottle adopted by the invention has the advantage that the amount of soil powder pumped by a vacuum pump is reduced by more than 80%.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the structure of a sample storage bottle 1 according to the present invention;
fig. 3 is a schematic view showing the structure of the bottle cap 3, the bottle cap knob 5 and the stacking stabilizer 6 according to the present invention;
FIG. 4 is a schematic view of the structure of the partition plate 2 in the present invention;
fig. 5 is a schematic view illustrating the structure of the cap plate 51 outside the through hole 31 of the bottle cap according to the present invention;
fig. 6 is a schematic structural view of the cap plate 51 away from the outside of the through hole 31 of the bottle cap in the present invention;
fig. 7 is a schematic view of the construction of panel 4 in the closure according to the present invention.
Detailed Description
The first embodiment is as follows: referring to fig. 1 to 7, the present embodiment is described, wherein the sample bottle for soil storage and freeze-drying of microorganism detection comprises a sample storage bottle 1, a partition plate 2, bottle lid 3, panel 4 in bottle lid knob 5 and two bottle lids, the uncovered setting of upper end bottleneck of sample storage bottle 1, division board 2 sets up in sample storage bottle 1 and cut apart into a plurality of storage area of equidimension with sample storage bottle 1, 3 suits of bottle lid are in the outside of 1 bottleneck of sample storage bottle, be equipped with a set of bottle lid through-hole 31 on the up end of bottle lid 3, the middle part of 3 up end of bottle lid rotates and is connected with bottle lid knob 5, the rigid coupling has apron 51 on bottle lid knob 5, apron 51 sets up the outside at bottle lid through-hole 31, panel 4 in two bottle lids are inlayed to the inboard parallel of bottle lid 3, one side on the panel 4 in the bottle lid is equipped with a set of interior panel through-hole 41, interior panel through-hole 41 on panel 4 in two bottle lids sets up in a crisscross way.
The inner panel through holes 41 of the upper bottle cap inner panel 4 and the bottle cap through holes 31 of the bottle cap 3 are arranged in a 180-degree staggered manner, and the inner panel through holes 41 of the lower bottle cap inner panel 4 and the inner panel through holes 41 of the upper bottle cap inner panel 4 are arranged in a 180-degree staggered manner.
And a handle 42 is fixedly connected to the middle part of the lower end face of the bottle cap inner panel 4 and used for installing and disassembling the bottle cap inner panel 4.
The bottle cap inner panel 4 is made of polytetrafluoroethylene materials, is circular, and is 8cm in diameter and 0.2cm in thickness.
The partition plate 2 is a movable part and can be directly placed in the sample storage bottle 1.
The shape of division board 2 is "rice" font, division board 2 includes three baffle, and the baffle is vertical to be set up, and the middle part of three baffle is coaxial crossing. The separation plate 2 is made of three pieces of high borosilicate glass which are coaxially intersected, and divides the sample storage bottle 1 into six areas with the same size. Each piece of high borosilicate glass of the partition plate 2 is 8cm high and 7cm long. Under the isolation of the partition board 2, the sample storage bottle 1 is divided into 6 areas to reduce the bulk volume of the soil sample and improve the freeze-drying rate.
The second embodiment is as follows: referring to fig. 1 to 7, the storage bottle 1 of the present embodiment is cylindrical, and the mouth of the storage bottle 1 extends outward in an arc-shaped curved surface. Technical features not disclosed in the present embodiment are the same as those of the first embodiment.
The sample storage bottle 1 is made of brown high borosilicate glass material, the size height is 10cm, the outer diameter is 8cm, the inner diameter is 7.5cm, and the top bottle mouth is in an arc shape and is curled outwards by 0.5 cm.
The body of the sample storage bottle 1 is provided with scale marks 11 along the height direction.
The body of the sample storage bottle 1 is provided with a sample information recording label 12.
The third concrete implementation mode: referring to fig. 1 to 7, the bottle cap 3 of the present embodiment is cylindrical, the lower end of the bottle cap 3 is open, two annular grooves 32 are formed in parallel on the inner circumferential sidewall of the bottle cap 3, and the outer edge of the inner panel 4 of the bottle cap is disposed in the annular grooves 32. The technical features not disclosed in the present embodiment are the same as those of the second embodiment.
The bottle cap 3 is made of polytetrafluoroethylene materials, and is 4cm in height, 8cm in inner diameter and 9cm in outer diameter.
And a sealing rubber strip is arranged at the bottom of the bottle cap 3 and at one side of the interface of the sample storage bottle 1.
The interval between the two ring grooves 32 is 1cm, and a sealing gasket is arranged in the ring groove 32 and used for fixing the embedded plate 4 in the bottle cap.
The fourth concrete implementation mode: the embodiment is described with reference to fig. 1 to 7, the bottle cap knob 5 of the embodiment includes a rotating shaft 52 and two shifting pieces 53, the rotating shaft 52 is vertically and fixedly connected to the middle of the upper end surface of the bottle cap 3, the two shifting pieces 53 are vertically arranged in a cross shape, the middle of the shifting piece 53 is sleeved on the rotating shaft 52, and the cover plate 51 is fixedly connected to the lower portion between the two shifting pieces 53. The technical features not disclosed in this embodiment are the same as those of the first, second, or third embodiment.
The bottle cap knob 5 is made of polytetrafluoroethylene materials, a rotating shaft 52 with the diameter of 0.5cm and the height of 1cm is arranged in the center of the knob, and the rotating shaft is poured in the center of the bottle cap 3.
The length of the poking piece 53 is 6cm, and the height is 1 cm.
The fifth concrete implementation mode: in the present embodiment, the cover plate 51 is shaped like a quarter fan, one side of the cover plate 51 is fixed to the lower end of one side of one of the levers 53, and the other side of the cover plate 51 is fixed to the lower end of the other lever 53, as described with reference to fig. 1 to 7. The technical features not disclosed in the present embodiment are the same as those of the fourth embodiment.
A fan-shaped cover plate 51 is arranged in a quarter area between the poking sheets 53, and the diameter of the cover plate 51 is 3cm, and the thickness of the cover plate is 0.2 cm.
The sixth specific implementation mode: the present embodiment is described with reference to fig. 1 to 7, in which the set of bottle cap through holes 31 in the present embodiment includes a plurality of bottle cap through holes 31 uniformly arranged in a matrix, and the plurality of bottle cap through holes 31 are arranged on the bottle cap 3 along a quarter sector area with a rotating shaft 52 as a center. The technical features not disclosed in the present embodiment are the same as those in the fifth embodiment.
The diameter of the sector area is 2cm, and the aperture of the bottle cap through hole 31 is 0.15 mm.
The seventh embodiment: the present embodiment is described with reference to fig. 1 to 7, and the set of inner panel through holes 41 of the present embodiment includes a plurality of inner panel through holes 41 uniformly arranged in a matrix, and the plurality of inner panel through holes 41 are arranged in a square area on one side of the inner panel 4. The technical features not disclosed in the present embodiment are the same as those of the sixth embodiment.
The size of the square area is 1.5cm multiplied by 1.5cm, and the aperture of the through hole 41 of the embedded plate is 0.15 mm.
The specific implementation mode is eight: referring to fig. 1 to 7, the present embodiment is described, and the soil storage and lyophilization sample bottle for microorganism detection according to the present embodiment further includes a stacking stabilizer 6, and the stacking stabilizer 6 is fixedly connected to an outer edge of an upper end surface of the bottle cap 3. The technical features not disclosed in this embodiment are the same as those of the first, second, third, fifth, sixth, or seventh embodiments.
The stacking stabilizing frame 6 is made of polytetrafluoroethylene materials, is poured around the bottle cap 3, is 3cm high and is arc-shaped to slightly clamp the sample storage bottle 1.
The specific implementation method nine: the embodiment is described with reference to fig. 1 to 7, and the stacking stabilizing frame 6 of the embodiment includes a plurality of limiting arc plates, the limiting arc plates are uniformly distributed along the circumferential direction of the bottle cap 3, and the lower ends of the limiting arc plates are fixedly connected to the outer edge of the upper end surface of the bottle cap 3. The technical features not disclosed in this embodiment are the same as those in the eighth embodiment.
The detailed implementation mode is ten: referring to fig. 1 to 7, the present embodiment is described, and the method for detecting a soil-stored and lyophilized sample bottle for microorganism detection according to the present embodiment includes the following steps:
the method comprises the following steps: firstly, placing a partition board 2 in a sample storage bottle 1, and then uniformly distributing collected soil samples into a plurality of areas partitioned by the partition board 2 in the sample storage bottle 1;
step two: installing two bottle cap inner panels 4 into a bottle cap 3, wherein inner panel through holes 41 on the upper bottle cap inner panel 4 and bottle cap through holes 31 on the bottle cap 3 are arranged in a 180-degree staggered mode, and inner panel through holes 41 on the lower bottle cap inner panel 4 and inner panel through holes 41 on the upper bottle cap inner panel 4 are arranged in a 180-degree staggered mode;
step three: rotating the bottle cap knob 5, rotating the cover plate 51 to the outer side of the bottle cap through hole 31 to enable the bottle cap through hole 31 to be in a closed state, and then covering the bottle cap 3 on the bottle mouth of the sample storage bottle 1;
step four: firstly, placing a sample storage bottle 1 into a refrigerator with the temperature of-20 ℃ for pre-freezing, then placing the sample storage bottle 1 into a freeze dryer, rotating a bottle cap knob 5, keeping a cover plate 51 away from a bottle cap through hole 31 to enable the bottle cap through hole 31 to be in an open state, stacking a plurality of sample storage bottles 1 along the height direction under the clamping of a stacking stabilizing frame 6, and starting the freeze dryer for freeze drying after the plurality of sample storage bottles 1 are placed.
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and applications of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims. It should be understood that features described in different dependent claims and herein may be combined in ways different from those described in the original claims. It is also to be understood that features described in connection with individual embodiments may be used in other described embodiments.
Claims (10)
1. A soil-stored and freeze-dried sample vial for use in microbiological testing, characterized by: the bottle cap comprises a sample storage bottle (1), a partition plate (2), a bottle cap (3), a bottle cap knob (5) and two bottle cap inner embedded plates (4), wherein the upper end bottleneck of the sample storage bottle (1) is open, the partition plate (2) is arranged in the sample storage bottle (1) and divides the sample storage bottle (1) into a plurality of storage areas with the same size, the bottle cap (3) is sleeved on the outer side of the bottleneck of the sample storage bottle (1), a group of bottle cap through holes (31) are formed in the upper end face of the bottle cap (3), the middle of the upper end face of the bottle cap (3) is rotatably connected with the bottle cap knob (5), a cover plate (51) is fixedly connected onto the bottle cap knob (5), the cover plate (51) is arranged on the outer side of the bottle cap through holes (31), the two bottle cap inner embedded plates (4) are parallelly embedded into the inner side of the bottle cap (3), a group of embedded plate through holes (41) are formed in one side of the bottle cap inner embedded plate (4), the through holes (41) of the embedded plates on the two bottle cap inner embedded plates (4) are arranged in a staggered mode.
2. The soil-storing and freeze-drying sample bottle for microorganism detection according to claim 1, characterized in that: the sample storage bottle (1) is cylindrical, and the bottle mouth of the sample storage bottle (1) is arc-shaped and extends outwards.
3. The soil-storing and freeze-drying sample bottle for microorganism detection according to claim 2, characterized in that: the bottle cap (3) is cylindrical, the lower end of the bottle cap (3) is arranged in an opening mode, two annular grooves (32) are formed in the inner circumferential side wall of the bottle cap (3) in parallel, and the outer side edge of the embedded plate (4) in the bottle cap is arranged in the annular grooves (32).
4. The soil-storing and freeze-drying sample bottle for microbiological detection according to claim 1, 2 or 3, characterized in that: bottle lid knob (5) are including pivot (52) and two plectrums (53), and the perpendicular rigid coupling in the middle part of bottle lid (3) up end of pivot (52), and two plectrums (53) are the vertical setting of cross form, and the middle part suit of plectrum (53) is on pivot (52), and apron (51) rigid coupling is in the lower part between two plectrums (53).
5. The soil-storing and freeze-drying sample bottle for microbiological detection according to claim 4, wherein: the cover plate (51) is in a quarter-sector shape, one side of the cover plate (51) is fixedly connected with the lower end of one side of one shifting piece (53), and the other side of the cover plate (51) is fixedly connected with the lower end of one side of the other shifting piece (53).
6. The soil-storing and freeze-drying sample bottle for microbiological detection as claimed in claim 5, wherein: the group of bottle cap through holes (31) comprise a plurality of bottle cap through holes (31) which are uniformly distributed in a matrix shape, and the bottle cap through holes (31) are formed in a quarter fan-shaped area on the bottle cap (3) by taking the rotating shaft (52) as the center of a circle.
7. The soil-storing and freeze-drying sample bottle for microbiological detection as claimed in claim 6, wherein: the group of the embedded plate through holes (41) comprise a plurality of embedded plate through holes (41) which are uniformly distributed in a matrix shape, and a plurality of the embedded plate through holes (41) are formed in one side of the internal embedded plate (4) along a square area.
8. The soil-storing and freeze-drying sample bottle for microorganism detection according to claim 1, 2, 3, 5, 6 or 7, characterized in that: the sample bottle for the soil storage and freeze-drying of the microorganism detection further comprises a stacking stabilizing frame (6), and the stacking stabilizing frame (6) is fixedly connected to the outer side edge of the upper end face of the bottle cap (3).
9. The soil-storing and freeze-drying sample bottle for microbiological detection as claimed in claim 8, wherein: the stacking stabilizing frame (6) comprises a plurality of limiting arc plates which are uniformly distributed along the circumferential direction of the bottle cap (3), and the lower ends of the limiting arc plates are fixedly connected to the outer side edge of the upper end face of the bottle cap (3).
10. The method for testing a soil-stored and lyophilized sample bottle for microorganism detection according to any one of claims 1 to 9, comprising the steps of:
the method comprises the following steps: firstly, placing a partition plate (2) in a sample storage bottle (1), and then uniformly distributing collected soil samples into a plurality of areas partitioned by the partition plate (2) in the sample storage bottle (1);
step two: installing two bottle cap inner panels (4) into a bottle cap (3), wherein an embedded plate through hole (41) on the upper layer bottle cap inner panel (4) and a bottle cap through hole (31) on the bottle cap (3) are arranged in a 180-degree staggered manner, and an embedded plate through hole (41) on the lower layer bottle cap inner panel (4) and an inner panel through hole (41) on the upper layer bottle cap inner panel (4) are arranged in a 180-degree staggered manner;
step three: rotating a bottle cap knob (5), rotating a cover plate (51) to the outer side of a bottle cap through hole (31) to enable the bottle cap through hole (31) to be in a closed state, and then covering a bottle cap (3) on the bottle mouth of the sample storage bottle (1);
step four: the method comprises the steps of firstly putting a sample storage bottle (1) into a refrigerator with the temperature of-20 ℃ for pre-freezing, then putting the sample storage bottle (1) into a freeze dryer, rotating a bottle cap knob (5), keeping a cover plate (51) away from a bottle cap through hole (31), enabling the bottle cap through hole (31) to be in an open state, stacking and placing a plurality of sample storage bottles (1) in a stacking stabilizing frame (6) along the height direction, and after the sample storage bottles are placed, starting the freeze dryer for freeze drying.
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| CN114476330B (en) | 2024-02-27 |
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