CN114468036B - Fresh-keeping preparation of okra, and preparation method and application thereof - Google Patents
Fresh-keeping preparation of okra, and preparation method and application thereof Download PDFInfo
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- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 claims abstract description 74
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- 238000004458 analytical method Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/157—Inorganic compounds
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides an okra fresh-keeping preparation, a preparation method and application thereof, wherein the okra fresh-keeping preparation is prepared by mixing hydrogen-rich water and melatonin in a specific proportion, so that the green color of okra can be better maintained, the degradation of okra cell walls can be effectively inhibited, the hardness, the weightlessness rate and the chromatic aberration a value of okra are maintained, black spots on the surface of okra are prevented, and the storage time of okra is remarkably prolonged. The okra preservation technology provided by the invention is simple and convenient to operate, low in cost, good in preservation effect, green and safe, beneficial to human health and environment protection, and very worth popularizing and applying.
Description
Technical Field
The invention belongs to the technical field of vegetable preservation, and relates to a fresh-keeping preparation of okra, a preparation method and application thereof.
Background
Okra is an annual herb, belonging to the family malvaceae and growing widely in tropical and subtropical countries. The fruit has tender meat quality, smooth taste, unique flavor, and has the effects of promoting digestion, reducing blood lipid, protecting liver, delaying aging, protecting vision and the like because the special high vitamin C, dietary fiber, calcium and low saturated fatty acid are considered as ideal health food. With the increasing attention of society to green and organic vegetables, okra is one of vegetables with nutrition and health care functions, and has received more and more attention.
Okra per se has high moisture content and large surface area, is extremely liable to lose water, age, brown stain and fibrosis, seriously affects commodity value, and has market price higher than most vegetables. Therefore, the study on the storage and preservation of okra has a certain practical application value. At present, the main focus is on the physical (air conditioning and refrigeration) aspects. The air conditioning can effectively improve the sensory quality of the harvested okra in the cold storage period and delay the aging of the okra. The vacuum refrigeration can obviously reduce the weight loss rate of okra, delay the occurrence of respiratory peaks and delay the reduction of the content of soluble sugar. However, the one-time investment of the air-conditioned cold store is large, and the storage cost is high; the logistics cost is high when the materials are stored at low temperature, and a large amount of manpower, material resources and financial resources are required to be input. The chemical method is limited because the chemical substance residues have toxic and side effects on human bodies. Therefore, it is important to find a simple, safe and effective biological fresh-keeping technology.
CN112021405a provides a green-to-red regulation method for tomatoes with high antioxidant power, but the method is not suitable for fresh-keeping of okra, and needs to provide reagents with various chemical components, which has adverse effects on human health and environmental protection.
Disclosure of Invention
In order to solve the problems, the invention provides an okra fresh-keeping preparation, a preparation method and application thereof, wherein the okra fresh-keeping preparation is prepared by mixing hydrogen-rich water and melatonin in a specific proportion, so that the green color of okra can be better maintained, the degradation of okra cell walls is inhibited, the hardness, the weightlessness rate and the chromatic aberration a value of okra are maintained, black spots on the surface of okra are prevented, and the preservation time of okra is remarkably prolonged. The okra fresh-keeping technology provided by the invention is simple, good in fresh-keeping effect and free of harmful chemical components, and is very suitable for popularization and application.
The hydrogen-rich water has the effects of treating various diseases in medical aspects, whitening in cosmetic aspects and promoting healing after skin injury caused by radioactivity, and has a certain protection effect on body lipid peroxidation caused by heavy load training in sports aspects. The method has wide application in agriculture, hydrogen can regulate the growth and development of plants, can improve the soil environment, and respectively uses hydrogen-rich water and tap water to irrigate angelica sinensis plants, and results show that the hydrogen-rich water irrigates the angelica sinensis, can obviously improve the plant height, leaf width and root system growth of the angelica sinensis and the yield of the angelica sinensis, and shows that the hydrogen-rich water irrigation has positive effects on the growth of the angelica sinensis. And for example, the hydrogen-rich water can regulate the growth and development of cucumber seedlings, balance oxidation reduction in cells and further reduce the degree of oxidative damage of plants under the stress of salt hypochondrium. These studies all show that hydrogen-rich water has good utilization and development space in agricultural planting.
Melatonin was first shown in 1958 to be extracted from bovine pine cone under the chemical name N-acetyl-5-methoxy tryptamine. In 1991, scientists detected melatonin in algae for the first time. As a healthy ingredient contained in the diet, many fruits and vegetables provide natural melatonin. At present, researches show that melatonin has a regulating effect on plant growth, not only can regulate plant seed germination, root growth, flowering and the like, but also can improve the resistance of plants to the external environment and reduce damage caused by pathogenic bacteria infection, and the melatonin can regulate and control the fruit aging process, improve the disease resistance and keep the quality of fruits and vegetables.
According to the invention, a great number of researches show that the mixed preparation prepared by mixing hydrogen-rich water and melatonin according to a specific proportion has a very good fresh-keeping effect on okra, can better keep the green color of okra, inhibit the degradation of okra cell walls, maintain the hardness, the weightlessness rate and the chromatic aberration a value of okra, prevent the surface of okra from black spots, and remarkably prolong the preservation time of okra.
The application of the hydrogen-rich water on the human body is very wide, which proves that the fresh-keeping treatment of the hydrogen-rich water has absolute safety. The melatonin is supplemented from outside the body, so that the melatonin level in the body can be maintained in a young state, the circadian rhythm is regulated and recovered, the sleep can be deepened, the sleep quality is improved, more importantly, the functional state of the whole body is improved, the life quality is improved, and the aging process is delayed. The safety of melatonin is also illustrated. Therefore, the fresh-keeping preparation provided by the invention has very good safety, is beneficial to human health and environmental protection, and is very suitable for popularization and application.
In one aspect, the invention provides a fresh-keeping preparation of okra, which consists of hydrogen-rich water and melatonin.
The okra fresh-keeping preparation provided by the invention is only composed of two active ingredients, namely hydrogen-rich water and melatonin, and does not contain other active ingredients, and the composition of the ingredients is very simple, safe and healthy.
Further, the concentration of the melatonin in the hydrogen-rich water is 50-300 mu mol/L.
Further, the hydrogen content in the hydrogen-rich water is 0.78Mm.
Further, the mixing ratio of the melatonin to the hydrogen-rich water is 0.2728g/8L of the hydrogen-rich water, and the melatonin composite hydrogen-rich aqueous solution with the melatonin concentration of 150 mu mol/L is prepared.
On the other hand, the invention provides a preparation method of the fresh-keeping preparation of okra, which comprises the following steps:
(1) Measuring distilled water, bubbling a hydrogen generator into the distilled water to reach a saturation level;
(2) Melatonin is weighed and dissolved in the hydrogen-rich water which reaches the saturation state.
Further, when the melatonin is dissolved in the hydrogen-rich water in the step (2), the concentration of the melatonin in the hydrogen-rich water is 50-300 mu mol/L.
Further, step (1) was to take 8L of distilled water, and bubbling into 8L of distilled water at 25℃for 2 hours and 40 minutes at a rate of 300 ml/min by a hydrogen generator to reach a saturation level.
Further, in the step (2), 0.2728g of melatonin is weighed and dissolved in the hydrogen-rich water which is saturated.
Further, in the prepared fresh-keeping preparation, the concentration of the hydrogen-rich water is about 0.78Mm at 25 ℃, and the concentration of the melatonin is 150 mu mol/L.
In yet another aspect, the present invention provides a use of a composition comprising hydrogen-enriched water and melatonin for preparing an okra preservative formulation.
Further, the composition maintains the hardness of okra by inhibiting the degradation of the cell wall of okra.
Further, the composition can inhibit degradation of cell walls of okra by inhibiting degradation of protopectin and cellulose in okra and inhibiting generation of soluble pectin in okra.
In still another aspect, the invention provides a method for preserving okra, which comprises the steps of soaking okra in the preservation preparation for 15min, and storing at 25 ℃ for more than 11 days.
The okra fresh-keeping preparation and the preparation method and application thereof have the following beneficial effects:
1. the green color of okra can be better maintained, the degradation of okra cell walls is inhibited, the hardness, the weightlessness rate and the chromatic aberration a value of okra are maintained, black spots on the surface of okra are prevented, and the preservation time of okra is obviously prolonged;
2. the preparation is simple, the operation is convenient, and the cost is low;
3. is green and safe, and is beneficial to human health and environmental protection.
Drawings
FIG. 1 is a graph showing comparison of okra states after 7 days of treatment with different preservatives;
fig. 2 is a graph showing comparison of okra states after 11 days of treatment with different preservatives.
Detailed Description
The following description of the preferred embodiments of the present invention in further detail with reference to the accompanying drawings, it should be noted that the following embodiments are intended to facilitate an understanding of the present invention, and are not intended to limit the invention in any way, and all of the features disclosed in the embodiments of the present invention, or all of the steps in the methods or processes disclosed, can be combined in any manner, except for mutually exclusive features and/or steps.
EXAMPLE 1 melatonin concentration in Hydrogen-rich water was 30. Mu. Mol/L
8L of distilled water was measured, and bubbling was carried out in 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes, after reaching the saturation level, 0.0546g of melatonin was weighed and dissolved in the saturated hydrogen-rich water to a concentration of 30. Mu. Mol/L, and analyzed by gas chromatography, and the freshly prepared hydrogen-rich water had a concentration of about 0.78Mm at 25℃as an okra preservative preparation.
Example 2 melatonin in hydrogen-rich water was mixed at a ratio of 50. Mu. Mol/L
8L of distilled water was measured, and bubbling was carried out in 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes, after reaching the saturation level, 0.0909g of melatonin was weighed and dissolved in the saturated hydrogen-rich water to a concentration of 50. Mu. Mol/L, and analyzed by gas chromatography, and the freshly prepared hydrogen-rich water had a concentration of about 0.78Mm at 25℃as an okra preservative preparation.
Example 3 melatonin in hydrogen-rich water was mixed at a ratio of 100. Mu. Mol/L
8L of distilled water was measured, and bubbling was carried out in 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes, after reaching the saturation level, 0.1819g of melatonin was weighed and dissolved in the saturated hydrogen-rich water to a concentration of 100. Mu. Mol/L, and analyzed by gas chromatography, and the freshly prepared hydrogen-rich water had a concentration of about 0.78Mm at 25℃as an okra preservative preparation.
Example 4 melatonin in hydrogen-rich water was mixed at a ratio of 150. Mu. Mol/L
8L of distilled water was measured, and bubbling was carried out in 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes, after reaching the saturation level, 0.2728g of melatonin was weighed and dissolved in the saturated hydrogen-rich water to a concentration of 150. Mu. Mol/L, and analyzed by gas chromatography, and the freshly prepared hydrogen-rich water had a concentration of about 0.78Mm at 25℃as an okra preservative preparation.
Example 5 melatonin in hydrogen-rich water was mixed at a ratio of 200. Mu. Mol/L
8L of distilled water was measured, and bubbling was carried out in 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes, after reaching the saturation level, 0.3638g of melatonin was weighed and dissolved in the saturated hydrogen-rich water to a concentration of 200. Mu. Mol/L, and analyzed by gas chromatography, and the freshly prepared hydrogen-rich water had a concentration of about 0.78Mm at 25℃as an okra preservative preparation.
Example 6 melatonin in hydrogen-rich water was mixed at a ratio of 300. Mu. Mol/L
8L of distilled water was measured, and bubbling was carried out in 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes, after reaching the saturation level, 0.5456g of melatonin was weighed and dissolved in the saturated hydrogen-rich water to a concentration of 300. Mu. Mol/L, and analyzed by gas chromatography, and the freshly prepared hydrogen-rich water had a concentration of about 0.78Mm at 25℃as an okra preservative preparation.
EXAMPLE 7 melatonin in the hydrogen-rich water was mixed at a ratio of 400. Mu. Mol/L
8L of distilled water was measured, and bubbling was carried out in 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes, after reaching the saturation level, 0.7276g of melatonin was weighed and dissolved in the saturated hydrogen-rich water to a melatonin concentration of 400. Mu. Mol/L, and analyzed by gas chromatography, and the freshly prepared hydrogen-rich water concentration was about 0.78Mm at 25℃as an okra preservative preparation.
Example 8 Hydrogen-enriched water alone, free of melatonin
8L of distilled water was measured, and bubbling was carried out into 8L of distilled water at a rate of 300 ml/min by a hydrogen generator (LH-300; shanghai Leu instruments Co., ltd.) at 25℃for 2 hours and 40 minutes to reach a saturation level, and the concentration of freshly prepared hydrogen-rich water was about 0.78Mm at 25℃by gas chromatography analysis, as an okra fresh-keeping preparation.
EXAMPLE 9 melatonin alone, free of Hydrogen-rich Water
0.2728g of melatonin is weighed in 8L of distilled water, and the concentration of the melatonin solution is 150 mu mol/L and is used as an okra fresh-keeping preparation.
EXAMPLE 10 blank control
Distilled water is used as okra fresh-keeping preparation.
Example 11 analytical detection
Fresh okra is purchased from Ningbo local, 2000 okra which are uniform in size, full in size and free of black spots and damages are selected and divided into 10 groups of 200 okra each. Okra fresh-keeping preparations prepared in examples 1 to 10 are respectively adopted for soaking for 15min in each group of okra. After the soaking is finished, 10 groups of okra are naturally dried in air at normal temperature, and finally are placed in a constant temperature and humidity incubator to be stored for 11 days at 25+/-1 ℃ and 80% -90% relative humidity. Samples were taken before (day 0) and after 5, 7, 9, 11 days of storage, respectively, and the cell wall composition, hardness, weight loss and color difference a values of okra of the control group and the treatment group were measured.
The detection method of okra cell wall components comprises the following steps: including detection of pectic substances and detection of cellulose content. Pectic substances are measured by a carbazole colorimetric method according to a Cao Jiankang method. And drawing and calculating a standard curve of pectin content by taking the mass of the treated galacturonic acid as an abscissa and the absorbance value at the wavelength of 530nm as an ordinate. And respectively adding the extracted protopectin and the soluble pectin 1.0mL of the extracting solution into a 25mL graduated test tube, measuring the protopectin and the soluble pectin content in the sample according to the operation steps of a standard curve, and repeating the measurement for three times. The cellulose content was determined by anthrone colorimetry with reference to method Wei Yingying. And drawing and calculating a standard curve of the cellulose content by taking the treated cellulose content as an abscissa and the absorbance value at the wavelength of 620nm as an ordinate. The diluted 1.0mL solution is added into a 25mL graduated test tube, the cellulose content in the sample is measured according to the operation steps of a standard curve, and the measurement is repeated three times.
The detection method of okra hardness comprises the following steps: the hardness of okra fruit was measured by using TMS-Touch physical property spectrometer (FTC Co., USA). 10 fruits were selected, and the measurement was performed at the maximum transverse diameter of the waist of the fruit, and the measurement was repeated three times, and the result was represented by N.
The detection method of the okra weight loss rate comprises the following steps: a mass loss method is adopted. 10 fruits are selected, taken out at the same time every day, weighed by a balance, and measured repeatedly three times. The percentage of the mass difference before and after storage to the mass of the sample before storage, i.e. the weight loss rate, was calculated.
The detection method of the okra chromatic aberration a value comprises the following steps: and (3) measuring the maximum transverse diameter of the waist of the okra fruit by using a color difference meter manufactured by Kagaku (Hangzhou) instruments, and recording data. (10 okra with the same hardness, color difference is measured and then hardness is measured.)
The test results obtained after 5, 7, 9 and 11 days of storage after the treatment are shown in tables 1 to 4.
TABLE 1 Abelmoschus esculentus cell wall composition, hardness, weight loss and color difference a value variation 5 days after different treatments
TABLE 2 Abelmoschus esculentus cell wall composition, hardness, weight loss and color difference a value variation 7 days after various treatments
TABLE 3 variation of okra cell wall composition, hardness, weight loss and color difference a values 9 days after various treatments
TABLE 4 Abelmoschus esculentus cell wall composition, hardness, weight loss and color difference a value variation 11 days after various treatments
As can be seen from tables 1 to 4, when hydrogen-rich water alone (example 8) or melatonin alone (example 9) was used, the effect of slightly keeping okra fresh was not remarkable. When the mixed preparation of melatonin and hydrogen-rich water is adopted, degradation of protopectin and cellulose in okra can be obviously inhibited, generation of soluble pectin in okra can be inhibited, and degradation of okra cell walls can be effectively inhibited by adopting the combination of melatonin and hydrogen-rich water, so that reduction of hardness can be effectively prevented, increase of weight loss rate can be prevented, increase of color difference a can be inhibited, and green color of okra can be effectively maintained. Especially when the concentration of melatonin in hydrogen-rich water is maintained within the range of 50-300 mu mol/L, the compounding synergy is particularly obvious. Of these, it is most preferable that the concentration of melatonin in hydrogen-rich water is 150. Mu. Mol/L (example 4), and this is particularly excellent in inhibiting degradation of protopectin and cellulose in okra, inhibiting formation of soluble pectin in okra, and maintaining hardness and weight loss rate, and also maintaining green color of okra.
Comparing examples 4, 8, 9, 10 alone, the okra of the fresh water control group (example 10) was stored at 25 ℃ for 11 days, the protopectin was reduced to 7.89mg/g, the soluble pectin was increased to 5.44mg/g, the cellulose was reduced to 3.01mg/g, the hardness was reduced by 13.3% relative to before storage, the weight loss rate was 12.61%, and the fruits had lost commercial properties completely; after okra (example 8) subjected to hydrogen-rich water treatment is stored at 25 ℃ for 11 days, the protopectin is reduced to 8.03mg/g, the soluble pectin is increased to 4.91mg/g, the cellulose is reduced to 3.54mg/g, the hardness is reduced by 7.0 percent relative to that before storage, and the weight loss rate is 10.34 percent; after storage of okra treated with melatonin (example 9) at 25 ℃ for 11 days, the protopectin is reduced to 8.07mg/g, the soluble pectin is increased to 5.02mg/g, the cellulose is reduced to 3.85mg/g, the hardness is reduced by 16.0% relative to that before storage, and the weight loss rate is 10.26%; in contrast, after okra treated with the hydrogen-rich water and melatonin (example 4) is stored at 25 ℃ for 11 days, the protopectin is reduced to 10.11mg/g, the soluble pectin is increased to 3.03mg/g, the cellulose is reduced to 5.75mg/g, the hardness is reduced by 3.0% relative to that before storage, and the weight loss rate is 8.95%. Thus, compared with the hydrogen-rich water alone and the melatonin alone, the hydrogen-rich water compound melatonin treatment can remarkably inhibit the reduction of hardness and the increase of weight loss rate of okra during the storage period at 25 ℃ and maintain the marketability of okra. The color difference a value of okra in the clear water control group (example 10) is obviously increased after the okra is stored for 9 days, and is 26.3 percent higher than that before the okra is stored, the color difference a value reflects the green degree of the appearance of the okra, the smaller the negative value is, the more green the okra is, the chlorophyll of the okra in the clear water control group is degraded during the storage period at 25 ℃, and the green is obviously reduced; the color difference a value of okra in the hydrogen-rich water treatment group (example 8) is increased by 1.3% after 9 days of storage relative to that of okra before storage, the color difference a value of okra in the melatonin treatment group after 9 days of storage is increased by 11.0% relative to that of okra before storage, and the color difference a value of okra in the hydrogen-rich water compound melatonin treatment group (example 4) after 9 days of storage is increased by only 1.06% relative to that of okra before storage. Compared with the independent hydrogen-rich water and independent melatonin treatment, the hydrogen-rich water compound melatonin treatment can keep the green color of okra.
In addition, the okra state pairs after 7 days of treatment with the formulations prepared in examples 4, 8, 9, and 10 are shown in fig. 1, and the okra state pairs after 11 days of treatment with the formulations prepared in examples 4, 8, 9, and 10 are shown in fig. 2. In both fig. 1 and fig. 2, a blank (formulation provided in example 10) was provided, and in fig. 1 and fig. 2, the effect of treating okra with the formulations provided in examples 4, 8, 9 was shown from left to right, respectively.
As can be seen from the results of fig. 1 and 2, the okra in the fresh water control group showed slight black spots on the surface after being stored at 25 ℃ for 7 days; the appearance of the okra in the compound treatment group and the hydrogen-rich water treatment group is not abnormal, and the okra in the melatonin treatment group still keeps a fresh state. After the okra in the clear water control group is stored at 25 ℃ for 11 days, obvious black spots appear on the surface of the okra, and the commodity is lost; and the surface of the okra in the compound treatment group, the hydrogen-rich water treatment group and the melatonin treatment group still has no black spots.
The application of the present invention is not limited thereto. Such as by its range of application in environmental protection. Various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention, and the scope of the invention should be assessed accordingly to that of the appended claims.
Claims (4)
1. The application of the composition in preparing a preservative preparation for inhibiting degradation of protopectin and cellulose in okra and inhibiting generation of soluble pectin is characterized in that the composition consists of hydrogen-rich water and melatonin, wherein the concentration of the melatonin in the hydrogen-rich water is 50-300 mu mol/L, the hydrogen content in the hydrogen-rich water is 0.78Mm, the mixing ratio of the melatonin to the hydrogen-rich water is 0.2728g/8L, and the melatonin composite hydrogen-rich aqueous solution with the melatonin concentration of 150 mu mol/L is prepared.
2. The use according to claim 1, wherein the preparation method of the preservative formulation comprises the steps of:
(1) 8L of distilled water is measured, and bubbling is carried out into 8L of distilled water for 2 hours and 40 minutes at 25 ℃ at the speed of 300 milliliters per minute by a hydrogen generator to reach the saturation level;
(2) 0.2728g of melatonin is weighed and dissolved in hydrogen-rich water which is in a saturated state, the concentration of the melatonin in the hydrogen-rich water is 50-300 mu mol/L, and in the prepared fresh-keeping preparation, the concentration of the hydrogen-rich water is 0.78Mm at 25 ℃, and the concentration of the melatonin is 150 mu mol/L.
3. The preservative preparation for use according to claim 1, which comprises hydrogen-rich water and melatonin, wherein the concentration of the melatonin in the hydrogen-rich water is 50-300 mu mol/L, the hydrogen content of the hydrogen-rich water is 0.78Mm, the mixing ratio of the melatonin to the hydrogen-rich water is 0.2728g/8L, and the melatonin composite hydrogen-rich aqueous solution with the melatonin concentration of 150 mu mol/L is prepared.
4. A method of preparing a preservative formulation according to claim 3, comprising the steps of:
(1) 8L of distilled water is measured, and bubbling is carried out into 8L of distilled water for 2 hours and 40 minutes at 25 ℃ at the speed of 300 milliliters per minute by a hydrogen generator to reach the saturation level;
(2) 0.2728g of melatonin is weighed and dissolved in hydrogen-rich water which is in a saturated state, the concentration of the melatonin in the hydrogen-rich water is 50-300 mu mol/L, and in the prepared fresh-keeping preparation, the concentration of the hydrogen-rich water is 0.78Mm at 25 ℃, and the concentration of the melatonin is 150 mu mol/L.
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