CN1144615A

CN1144615A - Producing animal feed additive using microbe and its manufacturing method

Info

Publication number: CN1144615A
Application number: CN94106402A
Authority: CN
Inventors: 张肇铭; 王梦亮; 刘继彪; 巩明; 邓孔融; 张肖洪
Original assignee: KANGQIANG BIOLOG TECHNOLOGY DE
Current assignee: Taiyuan Kangqiang Pharmaceutical Co., Ltd.
Priority date: 1994-06-21
Filing date: 1994-06-21
Publication date: 1997-03-12
Anticipated expiration: 2014-06-21
Also published as: CN1076595C

Abstract

A microbe method for producing feed additive for animals includes such steps as saccharifying aqueous solution of starch, natural souring and fermentation until its pH value is equal to 4.5, addition of solutions of trace elements and vitamines, regulating pH value to neutralization, inoculation of photosynthetic bacteria culture at 10-30 deg.C under light radiation by 1000-3000 Lux and control of oxygen dissolving at 0.1-1 ppm for 8-12 days to form culture containing about 10X10 to the power 8 units/ml of photosynthetic bacteria. Said additive can promote growing of animals and improve their immunity. If sodium selenite is added, a selenium-enriched additive is obtained.

Description

Utilize the animal feedstuff additive and the production method thereof of microorganisms producing

The present invention relates to a kind of animal feedstuff additive and production method thereof of utilizing the microorganism preparation.

At present existing multiple fodder additives, as yeast, hydrogen bacterium etc., the general productive rate of these additives is lower, and often component is also complete inadequately, can not satisfy present high protein content, the feed request that component is complete, CN87102074.2 disclose a kind of mixed fungus fermentation feed formulations and preparation thereof, but its protein content only is 30%.CN92108260.6 discloses a kind of by rumen bacteria, and zymocyte liquid and energy feed and protein fodder mix the all-nutritious active feeding stuff that forms.Its complex manufacturing process also relies on energy feed and protein fodder increases albumen and energy.

The inventor finds after deliberation: utilize photosynthetic bacterium the organic acid that the starch acidifying produces can be converted into the proteic mixed solution of the photosynthetic bacterial thallus that contains multiple amino acids and VITAMIN, contain 18 seed amino acids and multivitamin in this mixed solution, wherein vitamin B group, particularly B ₁₂Content far above yeast, also contain ubiquinone in addition, foretell compound and nucleic acid fragments such as quinoline pigment, be much better than yeast as fodder additives.

The object of the present invention is to provide a kind of culturing mixt and production method thereof that contains photosynthetic bacterial thallus.

Another object of the present invention is to provide with the described cultivation mixed solution of photosynthetic bacterial thallus that contains as animal feedstuff additive.

Among the present invention, being used to produce the microorganism that contains the photosynthetic bacterium culturing mixt is CGMCCNO.0192, in on February 22nd, 1993 in Chinese microbial bacteria preservation management committee common micro-organisms center (CGMCC) preservation, this culture is made up of 9 strain photosynthetic bacteriums, wherein Rhodospirillium1 strain, Rhodopscudomonas2 strain, Rodobactcr2 strain, Chromatium1 strain, the Chlorobium1 strain, red thiobacterium 2 strains.

The cultural method of photosynthetic bacterium mixed culture CGMCC NO.0192 is: add 1gKH in the 1000ml distilled water ₂PO ₄, 0.5MgCL ₂.6H ₂O, 0.1gCaCl ₂.2H ₂O, 1gNH ₂CL, 3gNaHCO ₃, 0.7Nag ₃SO ₄, 1gNaCL, 1ml do not have vitriol trace element solution and 1ml vitamin solution, and under conventional organic carbon source condition, regulating initial PH is 6.9, cultivates with the illumination anaerobic process, and light wave is the inclined to one side long wave of visible light, and intensity of illumination is 1000-3000lux, culture reddens gradually.It is to survey the absorption spectrum of its culture at the 630nm place that its growth detects, and along with the growth of bacterium liquid, the absorption of its 0.D630 increases gradually.

No vitriol trace element solution is: with 1.8gFeCl ₂.4H ₂O, 250mgCOCL ₂.6H ₂O, 10mgNICL ₂.6H ₂O, 10mgCaCL ₂.5H ₂O70mgMnCL ₂.4H ₂O, 100mgZnCL ₂, 500mgH ₃BO ₃, 30mgNa ₂MO ₄2H ₂O, 10mgNa ₂SeO ₃.5H ₂In the O dissolving 1000ml distilled water, the pH value of solution is adjusted to 2-3 with HCL.

Vitamin solution is to contain the 10mg vitamin H in the 100ml distilled water, 35mg niacinamide, 30mg dichloride thiamines element, 20mg calcium Para-Aminobenzoic, 10mg pyridoxal hydrochloride, 10mg calcium pantothenate, 5mg vitamins B ₁₂

The culturing mixt of photosynthetic bacterium described in the present invention contains 10 ⁹Individual/ml photosynthetic bacterium viable bacteria body.The production method of described culturing mixt is: the amidin of 10-40% (by weight), HCL accent PH with 1N is 2 to 3, boiled the back 0.5 to 2 hour, be preferably 1 hour, transfer PH to neutral with NaOH, self-souring is 8 to 48 hours under 10 to 30 degrees celsius, preferably 28 degrees centigrade of acidifyings are 8 hours, PH reduces to 4 to 5, adds to account for the trace element solution of liquid weight 1% and 0.1% vitamin solution, uses NaHCO ₃It is neutral transferring PH, and 20% the CGMCC NO.0192 photosynthetic bacterium that access accounts for weight is 1000 to 3000lux, and the best is 2000lux, under the illumination, dissolved oxygen is 0.1 to 1ppm, and the best is 0.5ppm condition bottom fermentation 8 to 12 days, obtains the culturing mixt of purple photosynthetic bacteria.The thalline content of photosynthetic bacterium is 10 * 10 ⁸Individual/ml is to about 60 * 10 ⁸Individual/ml, can be various foodstuff starch or COD replace at 2000 to 1000mg/L soybean wastewater starch wherein.

Trace element solution described in the invention and vitamin solution are specified among the embodiment 1.The using method of the photosynthetic bacterium culturing mixt described in the invention is specified among the embodiment 1.

Rich selenium described in the invention closes the bacterium fodder additives, be that the selenium in the trace element solution is increased to 400 to 1000ppm, use the same method and cultivate and get, organic selenium content is 200mg/L in its product, be used for feeding animals, selenium content in its animal muscle is 4.0 to 12.0mg/Kg, and the selenium content that is used for raising chicken, duck, egg can reach 2.0 to 6.0mg/Kg.

Technical process of the present invention is, with 10% to 30% amidin, transfer PH to 2 with INHCL, poach got saccharification liquid in 0.5 to 2 hour, and transferring PH with INNaOH is 7,10 to 30 degrees centigrade of self-sourings 8 to 48 hours, get acid fermentation liquid, add trace element solution 1%, vitamin solution 0.1%, and use NaHCO ₃Transfer PH to the neutral photosynthetic bacterium mixture substratum that gets, insert the CGMCC NO.0192 photosynthetic bacterium of liquid gross weight 20%, through dissolved oxygen 0.1-1ppm, intensity of illumination is 1000 to 2000Lux, temperature is 10 to 30 degrees centigrade and cultivated 8 to 10 days, promptly get the purple photosynthetic bacteria culturing mixt, and to contain the photosynthetic bacterium number be 10 * 10 ⁸To 60 * 10 ⁸Individual/ml.

The W-Gum aqueous solution of embodiments of the invention 1:30% is transferred PH to 2 with 1NHCL, and poach 1 hour is transferred PH to 7 with 1NNaOH, 28 degrees centigrade of self-sourings 8 hours, and PH reduces to 4.5, uses NaHCO ₃Transferring PH is 7, add and account for the trace element solution of liquid weight 1% and 0.1% vitamin solution, access accounts for the CGMCC NO.0192 photosynthetic bacterium of liquid weight 20%, 28 degrees centigrade of 2000Lux conditions, dissolved oxygen is controlled at 0.5ppm and cultivated 8 days, red photosynthetic bacterium cultivate mixed solution, wherein photosynthetic bacterium content is 4.6 * 10 ⁹Individual/ml.

Trace element solution used among the embodiment is: 1g KH ₂PO ₄, 0.5g Mgcl ₂.6H ₂O, 1.8g Fecl ₂.4H ₂O, 250mg Cocl ₂.6H ₂O, 10mg Nicl ₂.6H ₂O, 70mg Mncl ₂.4H ₂O, 100mg Zncl ₂, 500mg H ₃BO ₃, 30mg Na ₂Mo ₄.2H ₂O, 10mg Na ₂SeO ₃.5H ₂O, the 7.2g ethylenediamine tetraacetic acid (EDTA).Be dissolved in IL and steam in the water, transfer dissolving in PH to 2 hour from HCL.

Vitamin solution used among the embodiment is: contain the 10mg vitamin H in the 100ml distilled water, 35mg niacinamide, 30mg dichloride thiamines, 20mg para-amino benzoic acid, 10mg pyridoxal hydrochloride, 10mg calcium pantothenate, 5mg vitamins B ₁₂, the 50mg yeast extract powder.

The per-cent that photosynthetic bacterium culturing mixt among the embodiment is pressed feed 3% adds in the feed, can make fish improve output 3% to 30.23%, improves surviving rate 4.2% to 10%, and the bait subnumber descends 5.87% to 26.25%.Chick is one month earlier laid eggs, and surviving rate improves 2% to 5%, and unit material consumpting ratio contrast descends 2.78% to 8.73%.The whole process of feeding any medicine of need not feeding, day output increases by 7.2%, fryer hyperplasia 7.8%-18.73%.Above-mentioned photosynthetic bacterium culturing mixt cooperates by 3: 100 with water, allows chicken drink, and can improve laying hen output rate 4.2%.Improve surviving rate 4.7%.

Embodiments of the invention 2 are that sulphur concentration increases to 1g/L in the trace element that will implement in 1, and with the rich selenium animal feedstuff additive that same method is produced, selenium content is 207ug/ml.If be used to the chicken of feeding, using method is with embodiment 1, and the selenium content of fryer chicken is 4.8mg/Kg, and the selenium content of laying hen egg can reach 2.7mg/Kg.

Claims

1, a kind of method of producing the microorganism animal feedstuff additive, it is included under the growth conditions, in a kind of acid fermentation liquid, add the vitamin solution that accounts for its weight 1% trace element solution and 0.1%, it is neutral transferring ph, add the photosynthetic bacterium mixed culture that the people accounts for weight 20%, cultivated 8-12 days, and got animal feedstuff additive, its cell density can reach 10 * 10 ⁸To about 60 * 10 ⁸Individual/ml, described growth conditions comprises a kind of physiologically acceptable PH, temperature, dissolved oxygen and intensity of illumination.

2. pass through the method for claim 1, a kind of microorganism mixed culture of generation.

3, in the method for claim 1, wherein said bacterial cultures is a CGMCC No0192 photosynthetic bacterium mixed culture, wherein contains 9 strain photosynthetic bacteriums, and culture temperature is 10～35 degrees centigrade, dissolved oxygen 0.1～1ppm, and intensity of illumination is 1000～3000lux.

4, the method in the claim 1, acid fermentation liquid wherein is to adopt 10～40% amidin, through saccharification 0.5～1 hour, 10～30 degrees centigrade of self-sourings 8～48 hours and getting, its PH is 4～5.

5, the method in the claim 1, wherein trace element solution is meant the ethylenediamine tetraacetic acid (EDTA) inner complex that contains Fe, Mg, K, P, Zn, Mo, Mn, B, Se, Ni, Co, 11 kinds of trace elements.

6, the method in the claim 1, amidin wherein can also be with foodstuff starch such as corns, and the soybean wastewater that also available COD is 2000～10000mg/l replaces.

7, the method in the claim 1, wherein vitamin solution is meant and contains vitamin H, niacinamide, dichloride thiamines element, para-aminobenzoic acid, pyridoxal hydrochloride, calcium pantothenate, vitamins B ₁₂The solution of 7 kinds of VITAMIN and 1%～5% yeast extract powder.

8, the microorganism mixed culture in the claim 2, as the purposes of animal feedstuff additive, addition is 1.5～3.5% of a feed weight, also can directly be drunk by animal, with the proportioning of water be 3-5: 100.

9, a kind of animal feedstuff additive, Se content in the trace element solution in the claim 1 is increased to 400～1000ppm, method with claim 1, the photosynthetic bacterium fodder additives that contains organoselenium can be made, the meat products of 40-120mg/Kg and the eggs product of 20-60mg/Kg can be produced after using.