CN114425791A

CN114425791A - Quantitative cutter for agarose electrophoresis gel

Info

Publication number: CN114425791A
Application number: CN202210088399.8A
Authority: CN
Inventors: 陈芳龙; 张剑峰; 迟胜起; 曹欣然; 刘杰; 郭静静; 王子琦; 赵亮; 张兆良
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2022-01-25
Filing date: 2022-01-25
Publication date: 2022-05-03

Abstract

The invention relates to the technical field of electrophoresis gel processing, in particular to an agarose electrophoresis gel quantitative cutter which comprises a supporting plate and two fixed claws arranged on the bottom end face of the supporting plate, wherein the side face of the supporting plate is fixedly connected with a cross beam, the surface of the cross beam is sleeved with a batcher, the end, close to the supporting plate, of the outer surface of the batcher is fixedly connected with a cutter head, the side face of the cutter head is provided with a vertically arranged chute, and the cutter head is in sliding connection with a blade through the arranged chute.

Description

Quantitative cutter for agarose electrophoresis gel

Technical Field

The invention relates to the technical field of electrophoresis gel processing, in particular to an agarose electrophoresis gel quantitative cutter.

Background

The agarose gel electrophoresis technology is an indispensable DNA detection and recovery tool for modern biological laboratories. Agarose is a high molecular material, and the aqueous solution of the agarose is melted at high temperature and solidified at low temperature to form a porous structure after solidification, so that the agarose can be used for separating DNA molecules with different lengths. The teeth are inserted into the agarose gel before the agarose is solidified, and then the teeth are pulled out after the agarose is solidified, so that small holes with the same shape as the teeth can be formed on the agarose. Such teeth with a fixed arrangement are called combs. And these small holes appearing in the solidified agarose are called gel holes.

When agarose electrophoresis is used for detecting DNA samples, a solution containing DNA is transferred into a gel hole, and the DNA with negative charge enters a porous structure formed after agarose is solidified under the action of an electric field. In general, longer DNA molecules move more slowly in this structure. Therefore, DNA molecules of different lengths can be separated into bands at different positions in the gel.

At present, when the electrophoresis gel is manufactured, the prepared agarose gel needs to be poured into a concave gel-making mold frame for cooling, and then the electrophoresis gel block is taken out for use, but the specifications of the conventional mold frame are 11-hole (1/4), 25-hole (2/4) and 50-hole (4/4), so that when the electrophoresis gel which is lower than 11-hole, more than 11-hole and less than 25-hole or more than 25-hole and less than 50-hole is needed to be used, the electrophoresis gel needs to be manually cut for reducing waste, and in the cutting process, the phenomenon of inclination and saw-tooth shape of a gel cutting port can occur, the use efficiency of the electrophoresis gel is influenced, and even more waste can be caused.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides an agarose electrophoresis gel quantitative cutter.

In order to achieve the purpose of the invention, the invention adopts the technical scheme that:

the utility model provides an agarose electrophoresis gel ration cutterbar, includes the backup pad and installs two fixed paws in backup pad bottom end face, the side and the crossbeam fixed connection of backup pad, and the surface and the batcher of crossbeam cup joint, the end department and the tool bit fixed connection that the batcher surface is close to the backup pad, and the side of tool bit sets up the spout of vertical setting, makes tool bit and blade sliding connection through the spout that sets up.

Preferably, the outer surface of the cross beam is provided with scale marks for realizing a quantitative function.

Preferably, the end of the cross beam far away from the support plate is provided with a boss.

Preferably, the blade has a thickness of 0.5mm to 1 mm.

Preferably, the crossbeam is made by the telescopic movable rod, and outside sleeve and backup pad fixed connection, the inside slide bar of crossbeam keeps away from the end department of backup pad and runs through backup pad and tool bit fixed connection, and the lateral surface and the blade sliding connection of tool bit.

Preferably, the outer surface of the inner sliding rod is provided with scale marks.

The invention has the beneficial effects that:

the device for cutting gel provided by the invention can cut the electrophoresis gel with any number of gel holes, the cut electrophoresis gel has smooth cut and no sawtooth-shaped edge, a target strip is not easy to warp after electrophoresis, and the waste of the electrophoresis gel is avoided.

Drawings

FIG. 1 is a schematic structural view of embodiment 1 of the present invention;

FIG. 2 is a schematic structural view of example 2 of the present invention;

fig. 3 is a side view of the mounting structure of the blade and the support plate in the present invention.

Reference symbol comparison table: 10-support plate, 20-fixed claws, 30-beam, 40-batcher, 50-cutter head, 60-blade.

Detailed Description

The following further describes embodiments of the present invention with reference to the accompanying drawings. In which like parts are designated by like reference numerals.

It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.

In order to make the content of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention.

In a first embodiment of the invention shown in figures 1 and 3,

the invention discloses an agarose electrophoresis gel quantitative cutter, which comprises a support plate 10 and two fixed claws 20 arranged on the bottom end face of the support plate 10, wherein the side face of the support plate 10 is fixedly connected with a cross beam 30, the outer surface of the cross beam 30 is provided with scale marks for realizing a quantitative function, the end of the cross beam 30 far away from the support plate 10 is provided with a boss for preventing a batcher 40 from falling off and influencing the use efficiency in the pulling process, the surface of the cross beam 30 is sleeved with the batcher 40, the end of the outer surface of the batcher 40 close to the support plate 10 is fixedly connected with a cutter head 50 for ensuring the size of cut gel to the maximum extent, the side face of the cutter head 50 is provided with a vertically arranged chute, the cutter head 50 is in sliding connection with a blade 60 through the arranged chute, and the thickness of the blade 60 is 0.5mm-1 mm.

The skilled person will understand that: in the present case, as shown in fig. 3, the maximum height of the cutter head 50 and the cutter head 60 is necessarily greater than the height of the support plate 10, and the specific length is controlled by the opening length of the chute, so that the function of cutting the electrophoresis gel in the mold can be realized only, and will not be described more herein.

In a second embodiment of the invention shown in figure 2,

the difference from the first embodiment is that: crossbeam 30 is made by the telescopic movable rod, and outside sleeve and backup pad 10 fixed connection, and the end department that backup pad 10 was kept away from to inside slide bar is connected with tool bit 50 fixed connection, and the surface of inside slide bar is provided with the scale mark, and the lateral surface and the blade 60 sliding connection of tool bit 50, in this embodiment, utilize the flexible of movable rod, drive the removal of tool bit 50, and then realize the cutting of electrophoresis gel, can reduce the holistic area of cutterbar simultaneously.

In a third embodiment of the present invention, as a technical extension of this aspect:

two pulleys with self-locking structures can be additionally arranged in each fixed claw 20, so that the phenomenon of movement under stress can be avoided when the cutter is used, the cutter can be conveniently moved integrally at the edge of the mold shell, and the range of rubber cutting is enlarged.

The working principle is as follows: when the electrophoresis gel cutting device is used, firstly, the two fixed claws 20 are clamped at the edge of a gel making die (play a role in fixing), the beam 30 is placed right above electrophoresis gel to be cut, the size of the gel block to be cut is determined by adjusting the distance between the cutter head 50 and the fixed claws 20 in a sliding mode through the batcher 40, and after the size is determined, the blade 60 is pressed downwards to enable the blade 60 to cut into the gel block, so that the purpose of quantitatively cutting gel is achieved.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. The quantitative cutter for agarose electrophoresis gel is characterized in that: including backup pad (10) and install two fixed paws (20) at backup pad (10) bottom face, the side and crossbeam (30) fixed connection of backup pad (10), and the surface and the batcher (40) of crossbeam (30) cup joint, the department and tool bit (50) fixed connection of the end that batcher (40) surface is close to backup pad (10), and the spout of vertical setting is seted up to the side of tool bit (50), makes tool bit (50) and blade (60) sliding connection through the spout that sets up.

2. The agarose electrophoresis gel quantitative cutter of claim 1, wherein: the outer surface of the cross beam (30) is provided with scale marks for realizing the quantitative function.

3. The agarose electrophoresis gel quantitative cutter of claim 1, wherein: the end of the cross beam (30) far away from the supporting plate (10) is provided with a boss.

4. The agarose electrophoresis gel quantitative cutter of claim 1, wherein: the thickness of the blade (60) is 0.5mm-1 mm.

5. The quantitative cutter for electrophoresis gel according to any one of claims 1 to 3, wherein: crossbeam (30) are formed by the preparation of telescopic movable rod, and outside sleeve and backup pad (10) fixed connection, the inside slide bar of crossbeam (30) keeps away from the end department of backup pad (10) and runs through backup pad (10) and tool bit (50) fixed connection, and the lateral surface and blade (60) sliding connection of tool bit (50).

6. The agarose electrophoresis gel quantitative cutter of claim 5, wherein: the outer surface of the inner sliding rod is provided with scale marks.