CN114395589B

CN114395589B - Method and equipment for improving polymalic acid fermentation yield

Info

Publication number: CN114395589B
Application number: CN202210045936.0A
Authority: CN
Inventors: 李留兵; 夏军
Original assignee: Jiangsu Hongze Century Biotechnology Co ltd
Current assignee: Jiangsu Hongze Century Biotechnology Co ltd
Priority date: 2022-01-17
Filing date: 2022-01-17
Publication date: 2023-03-10
Anticipated expiration: 2042-01-17
Also published as: CN114395589A

Abstract

The invention discloses a method and equipment for improving polymalic acid fermentation yield, which comprises a first step of preparing seed liquid after activating and culturing strains, a second step of connecting the seed liquid into fermentation equipment for fermentation and culture, and a third step of facilitating mixed fermentation of the seed liquid by swinging the fermentation equipment.

Description

Method and equipment for improving polymalic acid fermentation yield

Technical Field

The invention relates to fermentation preparation of high polymalic acid, in particular to a method and equipment for improving fermentation yield of polymalic acid.

Background

The polymalic acid is a homopolymer high molecular polymer with malic acid as the only monomer, and belongs to polyester polymers. The polymalic acid structure has modified carboxyl suspended to produce specific action with natural ligand or synthetic ligand as one kind of molecular matrix to prepare polymalic acid ester and other derivatives, which provides material for new material research and may be used in preparing great amount of new polymer with special function. Researches find that the polymalic acid has the characteristics of good water solubility, chemical derivatization, degradability, renewability, biological safety and the like. In the field of biological pharmacy, polymalic acid can be used as a microcapsule material and a drug carrier. The polymalic acid has high water solubility and water absorption, can be used as a water absorption material, and can also be used as a cosmetic product. The polymalic acid has inhibitory effect on protease of some biological substances (such as pea, potato, egg white protein, etc.) and DNA polymerase of some microorganisms, and can prevent growth and reproduction of some organisms. Therefore, the product can also be used as a packaging material for food production and processing.

At present, two methods, namely a chemical synthesis method and a microbial fermentation method, are mainly used for producing the polymalic acid, the polymalic acid prepared by a biological way is mainly synthesized by microbial fermentation, the biosynthetic polymalic acid has the outstanding advantages, and through microbial fermentation, the products are all beta-type, the product molecular weight is high, the production conditions are mild, and the purity of the generated product is high. In fact, the growth of aureobasidium pullulans cells and the demand of the biosynthesis of polymalic acid for dissolved oxygen may differ, and therefore, the contradiction that different dissolved oxygen levels are required in different culture stages can be difficult to solve only by keeping constant rotation speed all the time in the fermentation process, resulting in long fermentation period, low yield and high production cost.

Disclosure of Invention

In order to solve the technical problems, the invention provides a method for improving the fermentation yield of polymalic acid, which comprises the following steps:

step one, activating and culturing strains to prepare seed liquid;

secondly, inoculating the seed liquid into fermentation equipment for fermentation culture;

and step three, the swing fermentation equipment is convenient to realize the mixed fermentation of the seed liquid.

The utility model provides an improve equipment of gathering malic acid fermentation output, fermentation equipment includes the base, two supports are installed to the symmetry on the base, and fixed mounting has a motor on one of them support, two be equipped with the fermenting case between the support, the axis body is installed to the tip of fermenting case, the axis body is installed between two supports, one side that the axis body is close to the motor is fixed with the extension rod, install on the motor and drive the wobbling rocking arm of extension rod.

Further, the cross section of box is fan-shaped structure, all be equipped with the opening on the inclined plane of box both sides, the cooperation is installed the end cover on the opening.

Further, the top of the inner side of the box body is provided with a protruding structure, the two sides of the protruding structure are provided with first guide plates, one side of each first guide plate, which is close to the protruding structure, is rotatably provided with a second guide plate, and one side of each second guide plate is in contact with the bottom surface of the inner side of the box body.

Furtherly, be equipped with the cavity in the protruding structure, wherein, the cavity mainly comprises the fan-shaped cavity of relative distribution's first fan-shaped cavity and second, is equipped with cylindrical cell body between first fan-shaped cavity and the fan-shaped cavity of second, through cylindrical cell body intercommunication between first fan-shaped cavity and the fan-shaped cavity of second, first fan-shaped cavity is located the fan-shaped cavity upside of second, the inside intercommunication of second inlet channel and second exhaust passage and fermentation box is passed through respectively to the both sides of first fan-shaped cavity, the outside intercommunication of first inlet channel and first exhaust passage and fermentation box is passed through respectively to the both sides of second fan-shaped cavity, wherein, first inlet channel, first exhaust passage, second inlet channel and second exhaust passage all do not communicate each other, first inlet channel and second exhaust passage are located same one side, first inlet channel and second inlet channel are located same one side, all install the check valve in first inlet channel, first exhaust passage, second inlet channel and the second exhaust passage.

Further, install the subassembly that admits air in the cavity, the subassembly that admits air divides into four cavitys with first fan-shaped cavity and second fan-shaped cavity.

Further, the subassembly of admitting air includes hammer block, sleeve pipe and pin rod, be equipped with the stopper on the lateral surface of pin rod, be equipped with the second arc wall on the sheathed tube inner wall, the sleeve pipe cup joints in the outside of pin rod, and stopper and second arc wall cooperation installation simultaneously, wherein, install reset spring between the side of stopper and second arc wall, the intraductal pile of cover is opened two first arc walls, the both ends of first arc wall all communicate with the sleeve pipe outside, install the check valve in the first arc wall, the hammer block includes metal block and body, be equipped with two metal blocks along the symmetry of body axis on the week side of body, open the logical groove that runs through the body in the both sides of metal block, the body cup joints in the sleeve pipe outside, the sleeve pipe and the pin rod cooperation of body and internally mounted are installed in cylindrical groove.

Furthermore, the two ends of the pin rod are fixed on the side wall of the fermentation box body, the outer side surface of the sleeve is provided with limiting convex edges distributed along the axis of the sleeve, and the inner side of the metal block is provided with limiting grooves matched with the limiting convex edges.

Furthermore, the cavity body positioned at one side of the two metal blocks passes through the first arc-shaped groove

The invention has the following beneficial effects:

1. according to the invention, the convex structure is arranged at the top of the inner side of the fermentation box body, so that the U-shaped storage cavity is formed in the fermentation box body, the culture solution is shaken left and right in the U-shaped storage cavity of the fermentation box body under the action of gravity in the swinging process of the fermentation box body, and the culture solution at the bottom layer moves upwards along the first guide plate and the second guide plate in the swinging process, so that the contact between the culture solution at the bottom layer and the air at the upper layer is conveniently realized, the oxygen content of the culture solution is improved, and the propagation of good-quality culture bacteria is facilitated.

2. The hammer body is kept in a vertical state, and the change of the space volumes at two sides of the hammer body in the installed cavity is changed in the swinging process of the arranged protruding structure, so that the air inlet and outlet effect is achieved, the exchange of air in the fermentation box body and air outside the fermentation box body is conveniently realized, and the oxygen demand in the microbial fermentation process is met.

3. The external air enters the interior of the fermentation box body through the long channel, so that the waste heat is realized, the temperature change range in the fermentation box body is reduced, and the growth of culture bacteria is facilitated.

Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the description below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic overall sectional structure of the present invention;

FIG. 3 is an enlarged schematic view of the structure at A in FIG. 2 according to the present invention;

FIG. 4 is a schematic diagram of the inclined cross-section of the fermenter according to the present invention;

FIG. 5 is an enlarged view of the structure at B of FIG. 4 according to the present invention;

FIG. 6 is a schematic diagram of the inclined cross-section of the fermentation tank of the present invention;

FIG. 7 is an enlarged view of the structure of FIG. 6 at C according to the present invention;

FIG. 8 is a schematic view of the base structure of the present invention;

in the drawings, the components represented by the respective reference numerals are listed below:

1. a base; 2. a support; 3. a motor; 31. a rocker arm; 4. a fermentation box body; 41. a raised structure; 411. a chamber; 43. a first baffle; 44. a second baffle; 45. a first air intake passage; 46. a first exhaust passage; 47. a second intake passage; 48. a second exhaust passage; 5. an end cap; 6. a shaft body; 61. an extension pole; 7. a hammer body; 71. a metal block; 72. a pipe body; 73. a through groove; 8. a sleeve; 81. a first arc-shaped slot; 82. a second arc-shaped slot; 9. a pin rod; 10. a one-way valve.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments. The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.

Example 1:

a method for improving the fermentation yield of polymalic acid,

step one, activating and culturing strains to prepare a seed solution;

inoculating the seed liquid to fermentation equipment for fermentation culture;

Example 2:

the utility model provides an improve equipment of gathering malic acid fermentation output, fermentation equipment includes base 1, and two supports 2 are installed to the symmetry on the base 1, and fixed mounting has motor 3 on one of them support 2, is equipped with fermenting case 4 between two supports 2, and axis body 6 is installed to the tip of fermenting case 4, and axis body 6 is installed between two supports 2, and one side that axis body 6 is close to motor 3 is fixed with extension rod 61, installs on the motor 3 and drives extension rod 61 wobbling rocking arm 31.

The cross section of the fermentation box body 4 is of a fan-shaped structure, openings are formed in the inclined planes of the two sides of the fermentation box body 4, and end covers 5 are installed on the openings in a matched mode.

The top of the inner side of the fermentation box body 4 is provided with a convex structure 41, the two sides of the convex structure 41 are provided with first guide plates 43, one side of each first guide plate 43, which is close to the convex structure 41, is rotatably provided with a second guide plate 44, and one side edge of each second guide plate 44 is in contact with the bottom surface of the inner side of the fermentation box body 4.

Be equipped with cavity 411 in protruding structure 41, wherein, cavity 411 mainly comprises the first fan-shaped cavity and the second fan-shaped cavity of relative distribution, be equipped with cylindrical cell body between first fan-shaped cavity and the second fan-shaped cavity, communicate through cylindrical cell body between first fan-shaped cavity and the second fan-shaped cavity, first fan-shaped cavity is located second fan-shaped cavity upside, the inside intercommunication of second inlet channel 47 and second exhaust passage 48 and fermentation box 4 is passed through respectively to the both sides of first fan-shaped cavity, the outside intercommunication of first inlet channel 45 and first exhaust passage 46 and fermentation box 4 is passed through respectively to the both sides of second fan-shaped cavity, wherein, first inlet channel 45, first exhaust passage 46, second inlet channel 47 and second exhaust passage 48 all do not communicate each other, first inlet channel 45 and second exhaust passage 48 are located same side, first inlet channel 46 and second inlet channel 47 are located same side, first inlet channel 45, first exhaust passage 46, second inlet channel 47 and second exhaust passage 48 are all installed check valve 10 in.

An air inlet assembly is installed in the chamber 411, and divides the first fan-shaped cavity and the second fan-shaped cavity into four cavities.

The subassembly that admits air includes hammer block 7, sleeve pipe 8 and pin rod 9, be equipped with the stopper on the lateral surface of pin rod 9, be equipped with second arc wall 82 on the inner wall of sleeve pipe 8, sleeve pipe 8 cup joints in the outside of pin rod 9, stopper and second arc wall 82 cooperation installation simultaneously, wherein, install reset spring between the stopper and a side of second arc wall 82, it has two first arc wall 81 to pile up to open in the sleeve pipe 8, the both ends of first arc wall 81 all communicate with the sleeve pipe 8 outside, install check valve 10 in the first arc wall 81, hammer block 7 includes metal block 71 and body 72, be equipped with two along the symmetrical metal block 71 of body 72 axis on the week side of body 72, logical groove 73 of running through body 72 is opened to the both sides of metal block 71, body 72 cup joints in the sleeve pipe 8 outside, body 72 and internally mounted's sleeve pipe 8 and pin rod 9 cooperation are installed in cylindrical groove.

The two ends of the pin rod 9 are fixed on the side wall of the fermentation box body 4, the outer side surface of the sleeve 8 is provided with a limiting convex edge distributed along the axis of the sleeve, and the inner side of the metal block 71 is provided with a limiting groove matched with the limiting convex edge.

The cavity on one side of the two metal blocks 71 passes through the first arc-shaped slot 81.

In the using process, the motor 3 is started, the rocker arm 31 and the extension rod 61 are driven to rotate by the motor 3, and the rotating extension rod 61 drives the shaft body 6 and the fermentation box body 4 arranged on the shaft body 6 to swing;

because the top of the inner side of the fermentation box body 4 is provided with the convex structure 41, a U-shaped storage cavity is formed inside the fermentation box body 4, in the swinging process of the fermentation box body 4, the culture solution swings left and right in the U-shaped storage cavity of the fermentation box body 4 under the action of gravity, and the culture solution at the bottom layer moves upwards along the first guide plate 43 and the second guide plate 44 in the swinging process, so that the contact between the culture solution at the bottom layer and the air at the upper layer is conveniently realized, the oxygen content of the culture solution is improved, and the breeding of sample culture bacteria is facilitated;

when the fermentation box body 4 is arranged as shown in fig. 4 and swings to the left side, the hammer body 7 is arranged to keep a vertical state, the sleeve 8 is arranged to be not rotated under the action of a limiting block and a return spring on the outer side surface of the pin rod 9, the hammer body 7 rotates relative to the sleeve 8, the rotating sleeve 8 seals one end opening of two first arc-shaped grooves 81, four formed cavities are not communicated with each other, the left cavity of the first sector-shaped cavity is compressed, air in the left cavity of the first sector-shaped cavity pushes a one-way valve 10 in a second exhaust channel 48 to be opened, air enters the fermentation box body 4, the right cavity of the first sector-shaped cavity is enlarged, meanwhile, the right cavity of the fermentation box body 4 is compressed, waste gas in the right cavity of the fermentation box body 4 pushes a one-way valve 10 in a second air inlet channel 47 to be opened, waste gas enters the right side of the first sector-shaped cavity, gas replacement in the fermentation box body 4 is facilitated, the left cavity of the second sector-shaped cavity is enlarged, the one-way valve 10 in the first air inlet channel 45 is opened, air outside the second air inlet channel 4 enters the right side, air in the left cavity, the left cavity is pushed by a negative pressure in the second sector-shaped cavity, gas outlet of the second sector-shaped cavity, and the second sector-shaped cavity is discharged outside the second sector-shaped cavity 46;

when the fermentation box body 4 is arranged and swings to the left as shown in fig. 6, the outer side surface of the sleeve 8 is provided with the limiting convex ribs distributed along the axis of the sleeve, the inner side of the metal block 71 is provided with the limiting grooves matched with the limiting convex ribs, the hammer body 7 in a vertical state pushes the sleeve 8 to compress the return spring so as to rotate around the pin rod 9, so that the cavity on one side of the metal block 71 is communicated through the first arc-shaped groove 81, the left space of the first sector-shaped cavity is increased, the left space of the second sector-shaped cavity is compressed, fresh air in the left space of the second sector-shaped cavity pushes the one-way valve 10 arranged in the first arc-shaped groove 81 to be opened and enter the left space of the first sector-shaped cavity, and the one-way valve 10 in the first air inlet channel 45 and the second air outlet channel 48 are simultaneously closed; meanwhile, the space on the right side of the first fan-shaped cavity is reduced, and the space on the right side of the second fan-shaped cavity is increased, so that the waste gas on the right side of the second fan-shaped cavity pushes the check valve 10 installed in the other first arc-shaped groove 81 to be opened to enter the space on the right side of the first fan-shaped cavity, and the waste gas is discharged next time.

In addition, the external air enters the interior of the fermentation box body 4 through the long channel, so that the waste heat is realized, the temperature change amplitude inside the fermentation box body 4 is reduced, and the fermentation box is convenient to be suitable for the growth of culture bacteria.

It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by one of ordinary skill in the art and related arts based on the embodiments of the present invention without any creative effort, shall fall within the protection scope of the present invention. Structures, devices, and methods of operation not specifically described or illustrated herein are not specifically illustrated or described, but are instead contemplated to be practiced in the art by those skilled in the art.

Claims

1. An equipment for improving the fermentation yield of polymalic acid, which comprises a base (1) and is characterized in that,

two brackets (2) are symmetrically arranged on the base (1), and a motor (3) is fixedly arranged on one bracket (2);

a fermentation box body (4) is arranged between the two brackets (2), a shaft body (6) is arranged at the end part of the fermentation box body (4), and the shaft body (6) is arranged between the two brackets (2);

an extension rod (61) is fixed on one side of the shaft body (6) close to the motor (3), and a rocker arm (31) for driving the extension rod (61) to swing is installed on the motor (3);

a raised structure (41) is arranged at the top of the inner side of the box body (4), first guide plates (43) are arranged on two sides of the raised structure (41), a second guide plate (44) is rotatably arranged on one side, close to the raised structure (41), of the first guide plate (43), and one side edge of the second guide plate (44) is in contact with the bottom surface of the inner side of the box body (4);

a cavity (411) is arranged in the protruding structure (41), wherein the cavity (411) is mainly formed by a first fan-shaped cavity and a second fan-shaped cavity which are distributed oppositely, a cylindrical groove body is arranged between the first fan-shaped cavity and the second fan-shaped cavity, and the first fan-shaped cavity and the second fan-shaped cavity are communicated through the cylindrical groove body;

the first fan-shaped cavity is positioned on the upper side of the second fan-shaped cavity, two sides of the first fan-shaped cavity are respectively communicated with the interior of the fermentation box body (4) through a second air inlet channel (47) and a second air outlet channel (48), and two sides of the second fan-shaped cavity are respectively communicated with the exterior of the fermentation box body (4) through a first air inlet channel (45) and a first air outlet channel (46);

wherein the first air inlet passage (45), the first exhaust passage (46), the second air inlet passage (47) and the second exhaust passage (48) are not communicated with each other, the first air inlet passage (45) and the second exhaust passage (48) are positioned on the same side, and the first exhaust passage (46) and the second air inlet passage (47) are positioned on the same side;

one-way valves (10) are arranged in the first air inlet channel (45), the first exhaust channel (46), the second air inlet channel (47) and the second exhaust channel (48);

an air inlet assembly is arranged in the cavity (411), and divides the first fan-shaped cavity and the second fan-shaped cavity into four cavities;

the air inlet assembly comprises a hammer body (7), a sleeve (8) and a pin rod (9), wherein a limiting block is arranged on the outer side surface of the pin rod (9), a second arc-shaped groove (82) is formed in the inner wall of the sleeve (8), the sleeve (8) is sleeved on the outer side of the pin rod (9), the limiting block is installed in cooperation with the second arc-shaped groove (82), a reset spring is installed between the limiting block and one side surface of the second arc-shaped groove (82), two first arc-shaped grooves (81) are stacked in the sleeve (8), two ends of each first arc-shaped groove (81) are communicated with the outer side of the sleeve (8), a one-way valve (10) is installed in each first arc-shaped groove (81), the hammer body (7) comprises a metal block (71) and a pipe body (72), two metal blocks (71) which are symmetrical along the axis of the pipe body (72) are arranged on the peripheral side of the pipe body (72), through grooves (73) which penetrate through the pipe body (72) are formed in two sides of the metal block (71), the pipe body (72) is sleeved on the outer side of the sleeve (8), and the inner side of the sleeve (8) and the sleeve (9) and the pin rod (8) is installed in cooperation with the sleeve;

two ends of the pin rod (9) are fixed on the side wall of the fermentation box body (4), the outer side surface of the sleeve (8) is provided with limiting convex edges distributed along the axis of the sleeve, and the inner side of the metal block (71) is provided with a limiting groove matched with the limiting convex edges;

the cavity body positioned at one side of the two metal blocks (71) passes through the first arc-shaped groove (81).

2. The apparatus for improving the fermentation yield of the polymalic acid according to claim 1, wherein the cross section of the box body (4) is of a fan-shaped structure, the inclined planes at the two sides of the box body (4) are provided with openings, and the openings are provided with end covers (5) in a matching manner.