CN114381376B - Culture medium for co-culture of algae and bacteria and co-culture method thereof - Google Patents

Culture medium for co-culture of algae and bacteria and co-culture method thereof Download PDF

Info

Publication number
CN114381376B
CN114381376B CN202111392134.9A CN202111392134A CN114381376B CN 114381376 B CN114381376 B CN 114381376B CN 202111392134 A CN202111392134 A CN 202111392134A CN 114381376 B CN114381376 B CN 114381376B
Authority
CN
China
Prior art keywords
culture medium
chlorella
extract
orange
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202111392134.9A
Other languages
Chinese (zh)
Other versions
CN114381376A (en
Inventor
张秀霞
冼健安
张泽龙
李军涛
王冬梅
鲁耀鹏
郑佩华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
Original Assignee
Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences filed Critical Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
Priority to CN202111392134.9A priority Critical patent/CN114381376B/en
Publication of CN114381376A publication Critical patent/CN114381376A/en
Application granted granted Critical
Publication of CN114381376B publication Critical patent/CN114381376B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to the technical field of microalgae culture, in particular to an algae and fungus co-culture medium and application thereof. The culture medium comprises water and the following components per liter: 40-50 ml of rice wine, 0.3-0.5g of trace element water-soluble fertilizer, 8.0-10.0g of crude salt, 1.0-1.5g of amino acid organic fertilizer, and water supplementing to 1L. The algae bacteria co-culture medium disclosed by the invention contains proper carbon sources, nitrogen sources, trace elements and growth factors, is comprehensive in nutrition, can ensure the normal growth requirements of chlorella and photosynthetic bacteria, effectively promotes the growth of chlorella and photosynthetic bacteria, and has better effect by adding orange extract and eleusine robusta leaf extract on the basis. The culture medium has the advantages of simple and easily obtained components, low price and simple preparation method, and is suitable for farmers to culture chlorella and photosynthetic bacteria in an autonomous and enlarged manner.

Description

Culture medium for co-culture of algae and bacteria and co-culture method thereof
Technical Field
The invention relates to the technical field of microalgae culture, in particular to an algae bacteria co-culture medium and a co-culture method thereof.
Background
Chlorella (Chlorella sp.) and photosynthetic bacteria (Photosynthetic Bacteria, PSB for short) are two types of safe and reliable microecologics commonly used in aquaculture processes. PSB can rapidly decompose organic matters in water to generate N, P and other small-molecule inorganic salts; the chlorella consumes CO in the water body 2 The method can quickly absorb and utilize the N, P and other nutrient substances in the water and release oxygen for the cultivated organisms to utilize, so that the PSB and the chlorella are matched for use together to more effectively accelerate the decomposition and utilization of water pollutants, and simultaneously can provide oxygen for the cultivated organisms. Based on the advantages of the chlorella and photosynthetic bacteria algae-bacteria symbiotic system, the advantages of the chlorella and photosynthetic bacteria algae-bacteria symbiotic system are complementary, so that the culture time is saved, the investment of culture equipment is reduced, the culture water environment can be effectively and safely improved, the occurrence of diseases is reduced, and the quality of cultured aquatic animals is improved.
Disclosure of Invention
In view of the above, the invention provides a culture medium for co-cultivation of algae and bacteria and application thereof. The culture medium is suitable for the growth of chlorella and photosynthetic bacteria, has comprehensive nutrition, can ensure the normal growth requirements of the chlorella and the photosynthetic bacteria, has simple and easily obtained components, low price and simple preparation method, and is suitable for farmers to independently enlarge and culture the chlorella and the photosynthetic bacteria.
In order to achieve the above object, the present invention provides the following technical solutions:
an algae bacteria co-culture medium comprises the following components per liter:
Figure BDA0003365215660000011
in some embodiments, each liter of medium comprises the following components:
Figure BDA0003365215660000012
Figure BDA0003365215660000021
in some embodiments, each liter of medium comprises the following components:
Figure BDA0003365215660000022
in some embodiments, the media of the present invention further comprise one of the following combinations:
orange powder and olive leaf powder;
or a combination of orange extract and olive leaf extract.
In the invention, the orange is preferably Hainan orange, the orange extract is an extract of Hainan orange, and further preferably an alcohol extract of Hainan orange; the extract of the leaf of the elemene lobata is preferably an alcohol extract of the leaf of the elemene lobata, and the alcohol extract can be extracted according to a conventional method in the field.
In some embodiments, the orange extract and the olive leaf extract are prepared by the following steps:
pulverizing orange and olive leaf, sieving with 80-100 mesh sieve, collecting powder, adding absolute ethanol according to a feed liquid mass ratio of 1:10, soaking and extracting for 48 hours, centrifuging for 5-8 min, collecting supernatant, and obtaining orange extract and olive leaf extract.
In some embodiments, the centrifugation is at a speed of 5000 to 6000rpm.
The rice wine can be bulk rice wine or bottled rice wine, and can be obtained commercially or prepared according to a method commonly used in the field. In the present invention, rice used for brewing rice wine is not particularly limited, and is preferably glutinous rice, and the degree of the rice wine is preferably 10 to 25vol%. In some embodiments, bulk rice wine is used, and the preparation method comprises the following steps: the glutinous rice wine is prepared by cooking glutinous rice, cooling, adding distiller's yeast, brewing in a jar for 7-12 days, and finally squeezing to obtain the turbid wine with alcohol content of 10-25 vol%.
In some embodiments, each liter of medium comprises the following ingredients:
Figure BDA0003365215660000023
Figure BDA0003365215660000031
or (b)
Figure BDA0003365215660000032
Wherein the orange powder is dried and crushed orange powder, the orange extract is preferably orange alcohol extract, the large leaf olive leaf powder is large leaf olive leaf, and the large leaf olive leaf extract is preferably alcohol extract.
In some embodiments, each liter of medium comprises the following components:
Figure BDA0003365215660000033
in some embodiments, each liter of medium comprises the following components:
Figure BDA0003365215660000034
Figure BDA0003365215660000041
the invention also provides application of the culture medium in promoting the growth of chlorella, light and bacteria.
Wherein the photosynthetic bacteria comprise at least one of common photosynthetic bacteria strains such as rhodopseudomonas palustris, rhodospirillum rubrum, rhodopseudomonas capsulata and the like.
The invention also provides a method for co-culturing chlorella and photosynthetic bacteria, which comprises the step of inoculating the chlorella and the photosynthetic bacteria to the algae bacteria co-culture medium.
The inoculation amount of the chlorella is 5%; the inoculation amount of the photosynthetic bacteria is 5%.
The culture medium suitable for the algae bacteria co-cultivation comprises proper carbon sources, nitrogen sources, trace elements and growth factors, has comprehensive nutrition, can ensure the normal growth requirements of the chlorella and the photosynthetic bacteria, has simple and easily obtained components, low price and simple preparation method, and is suitable for being used by farmers for the automatic expansion cultivation of the chlorella and the photosynthetic bacteria.
Drawings
FIG. 1 shows a FSC-SSC diagram of a chlorella and photosynthetic bacteria loss cell analyzer.
Detailed Description
The invention provides an algae bacteria co-culture medium and a co-culture method thereof. Those skilled in the art can, with the benefit of this disclosure, suitably modify the process parameters to achieve this. It is expressly noted that all such similar substitutions and modifications will be apparent to those skilled in the art, and are deemed to be included in the present invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those skilled in the relevant art that the invention can be practiced and practiced with modification and alteration and combination of the methods and applications herein without departing from the spirit and scope of the invention.
The test materials adopted by the invention are all common commercial products and can be purchased in the market.
The invention is further illustrated by the following examples:
example 1
A culture medium suitable for co-cultivation of algae bacteria, each liter of the culture medium comprises the following components:
Figure BDA0003365215660000042
Figure BDA0003365215660000051
the preparation method of the culture medium suitable for the co-culture of the algae bacteria comprises the following steps:
boiling water, and cooling to room temperature for standby;
weighing 500ml of water, sequentially weighing trace element water-soluble fertilizer, crude salt and amino acid organic fertilizer, adding into the water, stirring and dissolving all reagents, and filtering insoluble solids in the solution;
adding rice wine, supplementing residual water, and stirring uniformly to obtain the culture medium suitable for the algal fungus co-culture.
Example 2
A culture medium suitable for co-cultivation of algae bacteria, each liter of the culture medium comprises the following components:
Figure BDA0003365215660000052
the preparation method is the same as in example 1.
Example 3
A culture medium suitable for co-cultivation of algae bacteria, each liter of the culture medium comprises the following components:
Figure BDA0003365215660000053
the orange powder and the large leaf olive kernel leaf powder are powdery medicinal materials of small orange and large leaf olive leaves of Hainan through a 80-100-mesh sieve.
The preparation method of the culture medium suitable for the co-culture of the algae bacteria comprises the following steps:
boiling water, and cooling to room temperature for standby;
weighing 500ml of water, sequentially weighing trace element water-soluble fertilizer, crude salt, amino acid organic fertilizer, orange powder and elemene leaf powder, adding into water, stirring to dissolve all reagents, soaking for 2h, and filtering insoluble solids in the solution;
adding rice wine, supplementing residual water, and stirring uniformly to obtain the culture medium suitable for the algal fungus co-culture.
Example 4
A culture medium suitable for co-cultivation of algae bacteria, each liter of the culture medium comprises the following components:
Figure BDA0003365215660000061
the preparation method of the applicable algal fungus co-culture medium is the same as in example 3.
Example 5
The differences from example 4 are: the extract of Hainan orange and the extract of large leaf olive kernel are added, and the dosage of other components is the same, wherein the preparation method of the extract is as follows:
sun-drying Hainan orange and large leaf olive kernel leaves, taking the formula amount of Hainan orange and large leaf olive kernel leaves, crushing, sieving with a 80-100 mesh sieve, taking powder, adding an absolute ethanol solution according to a feed liquid mass ratio of 1:10, soaking for 48 hours, centrifuging at a rotating speed of 5000-6000 rpm for 5-8 min, and taking the supernatant to obtain Hainan orange and large leaf olive kernel leaf extracts.
The preparation method of the culture medium suitable for the co-culture of the algae bacteria comprises the following steps:
(1) Boiling water, and cooling to room temperature for standby;
(2) Weighing 500ml of water, sequentially weighing trace element water-soluble fertilizer, crude salt and amino acid organic fertilizer, adding into the water, and stirring to dissolve all reagents;
(3) Adding rice wine, hainan orange extract and large leaf olive leaf extract to supplement residual water, and stirring uniformly to obtain the culture medium suitable for algae bacteria co-cultivation.
Comparative example 1
A commonly used chlorella culture medium (aquatic No. 6) comprises the following components per liter of culture medium:
Figure BDA0003365215660000062
Figure BDA0003365215660000071
the preparation method of the culture medium comprises the following steps:
boiling water, and cooling to room temperature for standby;
weighing 500ml of water, sequentially weighing the culture medium components, adding the culture medium components into the water, and stirring for dissolution;
adding the rest water, and uniformly mixing to obtain the required culture medium;
comparative example 2
A commonly used photosynthetic bacteria culture medium comprises the following components per liter:
Figure BDA0003365215660000072
the preparation method of the medium was the same as in comparative example 1.
Comparative example 3
Each liter of culture medium comprises the following components:
Figure BDA0003365215660000073
the preparation method of the culture medium was the same as in example 3.
Comparative example 4
Each liter of culture medium comprises the following components:
Figure BDA0003365215660000081
the preparation method of the culture medium was the same as in example 3.
Test example 1:
the culture effects of the algal co-cultivation in the culture mediums of the above examples and comparative examples were compared.
100L of the culture medium is prepared according to the proportion and is filled in 120L of white plastic drums, and three parallel culture mediums are arranged. The Chlorella used in the test was the inventor to select purified Chlorella (Chlorella sp.) from a southwest water environment, and photosynthetic bacteria species were the inventor to select rhodopseudomonas palustris (Rhodopseudomonas palustris) from a penaeus vannamei culture pond, respectively, by inoculating 5% of activated Chlorella and photosynthetic bacteria species. The inoculated barrel is covered with a transparent plastic film, cultured under outdoor natural conditions, stirred for three times in the morning, in the middle and at night, sampled on the next day and the fourth day of the inoculation and culture respectively, and the quantity of algae and bacteria in the algae and bacteria co-culture system is measured by a flow cytometer. The results are shown in Table 1.
TABLE 1 Effect of different culture media on the growth of algal co-cultivation systems
Figure BDA0003365215660000082
Rhodospirillum rubrum (Rhodospirillum rubrum) was selected to replace Rhodopseudomonas palustris in an algae co-cultivation system, and the test method is the same as above, and the results are shown in Table 2.
TABLE 2 Effect of different media on the growth of algal co-cultivation systems
Figure BDA0003365215660000083
Figure BDA0003365215660000091
The results show that the culture medium of comparative examples 1-2 is unfavorable for the simultaneous growth of photosynthetic bacteria and chlorella, wherein the culture medium of comparative example 1 is unfavorable for the growth of photosynthetic bacteria, and the growth rate of chlorella is the slowest when the culture is performed using the culture medium of comparative example 2, which is unfavorable for the growth of chlorella. Comparative example 3 the absence of one rice wine as compared with example 4, as well as other ingredients, proves that comparative example 3, which lacks an important component rice wine, is detrimental to the growth of chlorella and photosynthetic bacteria; comparative example 4 the absence of an amino acid organic fertilizer as compared to example 4, as well as other ingredients, demonstrated that comparative example 4, which lacks an amino acid organic fertilizer of an important component, is equally detrimental to the growth of chlorella and photosynthetic bacteria.
The culture medium of examples 3-4 is added with Hainan orange and fructus Canarii albi, the cell numbers of chlorella and photosynthetic bacteria are obviously higher than those of comparative examples 1-4, and the culture medium of example 5 is added with Hainan orange extract and fructus Canarii albi extract, and the cell numbers of chlorella and photosynthetic bacteria are the highest, which is more beneficial to the growth of an algae bacteria co-culture system.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (6)

1. An algae co-culture medium is characterized in that each liter of the culture medium comprises the following components:
50-80 ml of rice wine;
0.5-1.0 g of trace element water-soluble fertilizer;
8.0-10.0g of crude salt;
1.0-1.5g of amino acid organic fertilizer;
0.02-0.05 g of orange powder;
0.01-0.02 g of elemene macrophylla leaf powder;
make up water to 1L;
or (b)
50-80 ml of rice wine;
0.5-1.0 g of trace element water-soluble fertilizer;
8.0-10.0g of crude salt;
1.0-1.5g of amino acid organic fertilizer;
0.02-0.05 ml of orange extract;
0.01-0.02 ml of olive leaf extract;
make up water to 1L;
the orange extract is an orange alcohol extract; the leaf extract of the elemene macrophylla is an alcohol extract of the leaf of the elemene macrophylla.
2. The algal co-culture medium according to claim 1, wherein the orange is citrus reticulata.
3. The algal co-culture medium according to claim 2, wherein the orange extract and the olive leaf extract are prepared by the following methods:
sun-drying orange and elemi megaphyllum leaves, crushing, sieving with a 80-100 mesh sieve, taking powder, adding absolute ethyl alcohol according to a feed liquid mass ratio of 1:10, soaking and extracting for 48 hours, centrifuging for 5-8 minutes, and taking the supernatant to obtain orange extract and elemi megaphyllum leaf extract.
4. The algal co-culture medium according to claim 3, wherein the rotational speed of the centrifugation is 5000-6000 rpm.
5. The use of the culture medium according to any one of claims 1 to 4 for promoting the growth of chlorella and photosynthetic bacteria;
the photosynthetic bacteria comprise at least one of rhodopseudomonas palustris, rhodospirillum rubrum and rhodopseudomonas capsulata.
6. A method for co-culturing chlorella and photosynthetic bacteria, which is characterized in that chlorella and photosynthetic bacteria are inoculated to the algal co-culture medium according to any one of claims 1 to 4;
the inoculation amount of the chlorella accounts for 5% of the volume of the culture medium; the inoculation amount of the photosynthetic bacteria accounts for 5% of the volume of the culture medium.
CN202111392134.9A 2021-11-19 2021-11-19 Culture medium for co-culture of algae and bacteria and co-culture method thereof Active CN114381376B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111392134.9A CN114381376B (en) 2021-11-19 2021-11-19 Culture medium for co-culture of algae and bacteria and co-culture method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111392134.9A CN114381376B (en) 2021-11-19 2021-11-19 Culture medium for co-culture of algae and bacteria and co-culture method thereof

Publications (2)

Publication Number Publication Date
CN114381376A CN114381376A (en) 2022-04-22
CN114381376B true CN114381376B (en) 2023-06-30

Family

ID=81196455

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111392134.9A Active CN114381376B (en) 2021-11-19 2021-11-19 Culture medium for co-culture of algae and bacteria and co-culture method thereof

Country Status (1)

Country Link
CN (1) CN114381376B (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120077250A1 (en) * 2010-09-23 2012-03-29 Ut-Battelle, Llc Method for culturing photosynthetic microorganisms on microbial cellulose
CN102628024A (en) * 2012-03-31 2012-08-08 广东绿百多生物科技有限公司 Co-culture method of phycomycetes for promoting plant growth
CN103098981B (en) * 2013-01-29 2015-04-08 重庆市畜牧科学院 Dewatered solid combined inoculant for increasing protein level of citrus pulp feed and preparation method of dewatered solid combined inoculant
TWI641316B (en) * 2017-08-11 2018-11-21 楊志彬 Organic Aquaculture Water Quality Improvement Control Package
CN111281898A (en) * 2020-02-14 2020-06-16 广东中科无抗养殖科技有限公司 Composite micro-ecological bacteria agent for epidemic prevention of live pigs and preparation method and application thereof
CN112094780B (en) * 2020-09-26 2022-02-08 中国水产科学研究院黄海水产研究所 Seawater spirulina-interstellar bacterium symbiotic system, construction method and application

Also Published As

Publication number Publication date
CN114381376A (en) 2022-04-22

Similar Documents

Publication Publication Date Title
US10293008B2 (en) Process for enhancing the viable counts of lactic acid bacteria and useful compositions thereof
CN102511847A (en) Preparation method of spirulina extract
CN111187731A (en) Biological bottom-improving algae-culturing water quality improving microbial inoculum and preparation method thereof
CN100563811C (en) The method of a large amount of preparations of a kind of microbe microcapsules
CN101829284B (en) Process for extracting and refining flavone in highland barley seedlings by enzymolysis-membrane ultrafiltration
CN107673481B (en) Aquaculture water body purifying agent and preparation method thereof
CN107410757B (en) Preparation method of composite liquid bait for artificial coral culture
CN101082038A (en) Preparation of holothurian-benefit bacterium and method for restoring sea cucumber cultivation pool environment
CN104605138A (en) Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof
CN114381376B (en) Culture medium for co-culture of algae and bacteria and co-culture method thereof
CN116355816A (en) Microorganism of fermented samara oil seed and blood lipid reducing composition thereof
CN110468087B (en) Simple and easy-to-prepare photosynthetic bacteria culture medium and preparation method thereof
CN103103127A (en) Culture method for microalgae
CN108384720A (en) A method of it is flocculated using combination flocculant and harvests chlorella pyrenoidosa
CN110663601B (en) Method for controlling vibrio quantity in prawn culture system
CN107413821A (en) A kind of method that the thermophilic lysis bacterium of immobilization is used for antibiotic bacterium dregs minimizing
CN110150505B (en) Lactic acid bacteria complexing agent for removing earthy smell of eriocheir sinensis and preparation method thereof
CN109609384B (en) Chlorella sorokiniana TX strain and high-density rapid culture method thereof
CN112794774A (en) Composite organic liquid biological bacterial fertilizer applied to grape planting and preparation method thereof
CN113249425A (en) Method for improving high yield of astaxanthin by rhodotorula benthica strain
CN105779253A (en) Hawthorn-flavored honey vinegar and preparation method thereof
CN111218416A (en) Complex bacterium fermentation inoculant, composition, fermentation method and application thereof
CN114656301B (en) Chlorella biofertilizer for promoting growth and improving disease resistance
CN108740556A (en) A kind of nutrition fertilizer for aquaculture
CN114287521B (en) Compound enzymolysis preparation method of laminarin for replacing fish meal for cottonseed protein compound feed and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant