CN114381335A - Preparation method of lemongrass essential oil - Google Patents
Preparation method of lemongrass essential oil Download PDFInfo
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- CN114381335A CN114381335A CN202210062056.4A CN202210062056A CN114381335A CN 114381335 A CN114381335 A CN 114381335A CN 202210062056 A CN202210062056 A CN 202210062056A CN 114381335 A CN114381335 A CN 114381335A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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Abstract
The invention provides a preparation method of lemongrass essential oil, belongs to the technical field of plant essential oil extraction, and solves the problems of low extraction rate of the lemongrass essential oil, serious loss of active ingredients, high cost and the like in the prior art. The preparation method of the lemongrass essential oil comprises the steps of freezing, freeze drying, crushing, alcohol extraction, ultrasonic treatment, centrifugation, filtration, distillation and the like, effectively avoids the loss of active substances of the lemongrass essential oil and the conditions of hydrolysis, emulsification and the like which possibly occur in the extraction method, can effectively improve the stability and purity of the essential oil, can realize the separation of the essential oil more quickly and efficiently, and plays a certain role in protecting the active substances. The preparation method provided by the invention improves the extraction efficiency of the lemongrass essential oil, the final extraction rate of the lemongrass essential oil can reach 6.7% under the condition of the preparation method, the content of the main active ingredient, namely citral, can reach 52%, and the extraction efficiency and the essential oil yield are effectively improved.
Description
Technical Field
The invention relates to the technical field of plant essential oil extraction, and particularly relates to a preparation method of lemongrass essential oil.
Background
Cymbopogon citratus (also called citronella and lemon grass) is originally produced in tropical and subtropical regions of Asia and is widely cultivated in multiple provinces of south China and southwest China at present. The lemongrass has wide application and is applied to the fields of food cooking, seasoning making, chemical component extraction and synthesis and the like. As a natural herbaceous plant, the lemongrass has certain application potential in the extraction and application of plant essential oil. The lemongrass essential oil has unique lemon fragrance, mainly comprises aldehydes, alcohols, lipids, alkenes and other active compounds, wherein the citral is used as a main component of the lemongrass essential oil, has good antioxidant and antibacterial effects, and is used as a natural plant essential oil, and the application of the lemongrass essential oil in the fields of current food processing, cosmetic production, medicine preparation and the like is gradually improved.
The current extraction method of natural plant essential oil mainly comprises the traditional squeezing method, steam distillation method, organic solvent extraction method and the like, and has the problems of high energy consumption, long extraction time, large loss of active ingredients, low oil yield and the like. At the same time, some high and new extraction techniques such as supercritical CO are applied2The extraction technology, the subcritical water extraction technology and the microwave-assisted extraction technology can effectively improve the extraction efficiency and the recovery rate of the essential oil, but the cost is too high, the operation is complex, and the method is not suitable for industrial production.
CN106190585A discloses a method for extracting lemongrass essential oil by microwave radiation, which effectively improves extraction efficiency and extraction rate of essential oil through the action of microwave radiation, but the reaction temperature is too high, the heating time is too long, degradation loss of active ingredients in essential oil may be caused, various natural active compounds exist in the lemongrass essential oil, unstable characteristics such as easy oxidation, easy decomposition and easy volatilization generally exist, and long-time heat treatment can cause loss of the active substances.
CN105861158A discloses a method for extracting lemongrass essential oil by combining steam distillation and molecular distillation, which prepares the lemongrass essential oil with higher extraction efficiency in a pure physical organic solvent-free mode, but has higher cost and is not suitable for industrial production.
CN106336950A discloses a method for extracting lemongrass essential oil by combining petroleum ether extraction and ultrasonic technology, however, the petroleum ether used in the scheme has toxicity and certain potential safety hazard.
Disclosure of Invention
The invention aims to provide a preparation method of lemongrass essential oil, which aims to solve the problems of low extraction rate, serious loss of active ingredients, high cost and the like of the lemongrass essential oil in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of lemongrass essential oil, which comprises the following steps:
s1, sequentially freezing, freeze-drying and crushing the lemongrass to obtain freeze-dried lemongrass powder;
s2, mixing the freeze-dried lemongrass powder with an alcohol solution, and extracting to obtain lemongrass pulp;
s3, sequentially carrying out ultrasonic treatment, centrifugation and filtration on the lemongrass pulp to obtain filtrate;
s4, distilling the obtained filtrate to obtain the lemongrass essential oil.
Preferably, the freezing temperature in the step S1 is-70 to-90 ℃ and the time is 0.5 to 1.5 d.
Preferably, the freeze drying in the step S1 is vacuum freeze drying, the temperature of the vacuum freeze drying is-30 to-50 ℃, and the time of the vacuum freeze drying is 1.5 to 2.5 days.
Preferably, the alcohol solution in step S2 is an ethanol solution, and the volume concentration of the ethanol solution is 90-99%;
the mass volume ratio of the freeze-dried citronella grass powder to the ethanol solution is 1 g: 20-30 ml.
Preferably, the extraction process in step S2 is accompanied by stirring, and the rotation speed of the stirring is 50-150 rpm.
Preferably, the ultrasonic treatment in the step S3 is intermittently performed, the power of the ultrasonic treatment is 240-280W, the total time of the ultrasonic treatment is 20-30 min, and the interval time of the intermittent operation is 1-3S.
Preferably, the temperature of the ultrasonic treatment in the step S3 is-5 to 5 ℃.
Preferably, the rotation speed of the centrifugation in the step S3 is 8000-10000 rpm, and the centrifugation time is 10-15 min.
Preferably, the mesh number of the filtration in the step S3 is 80-120 meshes.
Preferably, the distillation in the step S4 is reduced pressure rotary distillation, the temperature of the reduced pressure rotary distillation is 60-65 ℃, the rotation speed of the reduced pressure rotary distillation is 50-150 rpm, the time of the reduced pressure rotary distillation is 20-30 min, and the vacuum degree of the reduced pressure rotary distillation is 0.05-0.1 MPa.
The invention has the technical effects and advantages that:
the invention provides a preparation method of lemongrass essential oil, which avoids the loss of active substances of the lemongrass essential oil and the hydrolysis and emulsification phenomena possibly occurring in the extraction process, and can effectively improve the stability and purity of the essential oil; the invention takes ethanol as an extracting agent, can avoid the problem of residual toxicity of the solvent, and can continue to recycle, thereby improving the economic benefit of production; the preparation method can quickly and efficiently separate the essential oil, and the low-temperature condition in the ultrasonic process can relieve the heat generation phenomenon in the ultrasonic treatment process, thereby playing a certain protection role on active substances. The preparation method provided by the invention improves the extraction efficiency of the lemongrass essential oil, the final extraction rate of the lemongrass essential oil can reach 6.7% under the condition of the preparation method, and the extraction efficiency and the essential oil yield are effectively improved.
Detailed Description
The invention provides a preparation method of lemongrass essential oil, which comprises the following steps:
s1, sequentially freezing, freeze-drying and crushing the lemongrass to obtain freeze-dried lemongrass powder;
s2, mixing the freeze-dried lemongrass powder with an alcohol solution, and extracting to obtain lemongrass pulp;
s3, sequentially carrying out ultrasonic treatment, centrifugation and filtration on the lemongrass pulp to obtain filtrate;
s4, distilling the obtained filtrate to obtain the lemongrass essential oil.
In the present invention, the lemongrass is preferably fresh lemongrass.
In the present invention, before the freezing step in step S1, a step of cutting the lemongrass is preferably further included, and the specific operation of the cutting is not particularly limited in the present invention, and a cutting method conventional in the art may be adopted; the preferred fragment of the cymbopogon citratus obtained by cutting is 1-2 cm; in the invention, the cutting can facilitate the refrigeration of the lemongrass and improve the refrigeration efficiency. In the present invention, the freezing temperature is preferably-70 to-90 ℃, more preferably-75 to-85 ℃, and still more preferably-78 to-82 ℃; the freezing time is preferably 0.5 to 1.5 days, more preferably 0.8 to 1.2 days, and still more preferably 0.9 to 1.1 days. The freeze drying is carried out after the freezing, the freeze drying is preferably vacuum freeze drying, the temperature of the vacuum freeze drying is preferably-30 to-50 ℃, more preferably-35 to-45 ℃, and further preferably-40 to-42 ℃, and the time of the vacuum freeze drying is preferably 1.5 to 2.5d, more preferably 1.7 to 2.3d, and further preferably 1.9 to 2.1 d.
After the freeze drying, the freeze-dried citronella grass powder is obtained by crushing. In the invention, the crushing is preferably carried out in a powder grinding mode by using a multifunctional crusher.
After the freeze-dried citronella grass powder is obtained, the freeze-dried citronella grass powder is mixed with an alcoholic solution and extracted to obtain citronella grass slurry. In the invention, the alcohol solution is preferably an ethanol solution, and the volume concentration of the ethanol solution is preferably 90-99%, more preferably 92-98%, and still more preferably 94-96%; the mass-volume ratio of the freeze-dried citronella grass powder to the alcohol solution is preferably 1 g: 20-30 ml, more preferably 1: 22-28, and still more preferably 1: 24-26; the extraction process is preferably accompanied by stirring, the rotation speed of the stirring is preferably 50-150 rpm, more preferably 60-130 rpm, and still more preferably 80-120 rpm, the extraction time is preferably 2-8 h, more preferably 3-7 h, and still more preferably 4-6 h; the extraction is preferably carried out at room temperature.
After the lemongrass pulp is obtained, the lemongrass pulp is subjected to ultrasonic treatment, centrifugation and filtration in sequence to obtain filtrate. In the invention, the ultrasonic treatment is preferably carried out intermittently, the interval time of the intermittent operation is preferably 1-3 s, more preferably 1.5-2.5 s, the interval working time of the intermittent operation is preferably 1-3 s, more preferably 1.5-2.5 s, and the total time of the ultrasonic operation is preferably 25-30min, more preferably 28-29 min; the equipment adopted by the ultrasonic treatment is preferably an ultrasonic cell disruptor; the power of the ultrasonic treatment is preferably 240-280W, more preferably 250-270W, and the total time of the ultrasonic treatment is preferably 20-30 min, more preferably 22-28 min, and more preferably 24-26 min; the temperature of the ultrasonic treatment is preferably-5 ℃, further preferably-3 ℃, and further preferably-2-1 ℃, and the temperature control mode is preferably an ice bath; in the step S3, the rotating speed of the centrifugation is preferably 8000-10000 rpm, and the time of the centrifugation is preferably 10-15 min; in the step S3, the filtering is preferably performed by using a filter screen, and the filtering mesh number is preferably 80-120, and more preferably 90-110.
After the filtrate is obtained, the obtained filtrate is distilled to obtain the lemongrass essential oil. In the invention, the distillation is preferably reduced pressure rotary distillation, the reduced pressure rotary distillation is preferably carried out under the condition of constant temperature water bath, and the constant temperature is preferably controlled at 60-65 ℃, more preferably at 61-64 ℃, and still more preferably at 62-63 ℃; the rotation speed of the reduced pressure rotary distillation is preferably 50-150 rpm, more preferably 60-120 rpm, and still more preferably 80-120 rpm, the time of the reduced pressure rotary distillation is preferably 20-30 min, more preferably 22-28 min, and still more preferably 24-26 min, and the vacuum degree of the reduced pressure rotary distillation is preferably 0.05-0.1 MPa.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Cutting fresh lemongrass, freezing at-80 deg.C for 1d, vacuum freeze-drying at-40 deg.C for 2d to obtain freeze-dried lemongrass material, pulverizing with multifunctional pulverizer at 40000rpm for 5min to obtain lyophilized lemongrass powder;
weighing 10g of pulverized lyophilized Lemongrass powder, adding 250ml of 95% ethanol solution, and magnetically stirring at 100rpm at room temperature for 5h to obtain Lemongrass slurry.
And placing the obtained lemongrass serous fluid in an ice bath, carrying out ultrasonic treatment by an ultrasonic cell disruptor, wherein the ultrasonic power is 260W, the ultrasonic time is 30min, the working interval is 2s, and the ultrasonic working time is 15min, centrifuging the serous fluid at 8000rpm for 10min after the ultrasonic treatment is finished, and filtering by a 100-mesh filter screen to obtain a filtrate.
And carrying out reduced pressure rotary distillation on the filtrate under the condition of constant-temperature water bath, wherein the rotary evaporation temperature is 60 ℃, the rotary evaporation speed is 100rpm, the rotary evaporation time is 25min, and the vacuum degree is 0.08MPa, and obtaining the lemongrass essential oil after the rotary evaporation is finished.
Example 2
Cutting fresh lemongrass, freezing at-70 deg.C for 0.5d, vacuum freeze-drying at-30 deg.C for 1.5d to obtain freeze-dried lemongrass material, pulverizing with multifunctional pulverizer at 40000rpm for 5min to obtain lyophilized lemongrass powder;
weighing 10g of pulverized lyophilized Lemongrass powder, adding 250ml of 95% ethanol solution, and magnetically stirring at 150rpm at room temperature for 6h to obtain Lemongrass slurry.
And (2) placing the lemongrass slurry in an ice bath, carrying out ultrasonic treatment by an ultrasonic cell disruptor, wherein the ultrasonic power is 240W, the ultrasonic time is 25min, the working interval is 2s, and the ultrasonic working time is 12.5min, centrifuging the slurry at 10000rpm for 15min after the ultrasonic treatment is finished, and filtering by a 100-mesh filter screen to obtain a filtrate.
And carrying out reduced pressure rotary distillation on the filtrate under the condition of constant-temperature water bath, wherein the rotary evaporation temperature is 65 ℃, the rotary evaporation speed is 100rpm, the rotary evaporation time is 25min, and the vacuum degree is 0.07MPa, and obtaining the lemongrass essential oil after the rotary evaporation is finished.
Example 3
Cutting fresh lemongrass, freezing at-90 deg.C for 0.5d, and vacuum freeze-drying at-30 deg.C for 2.5d to obtain freeze-dried lemongrass material;
weighing 10g of pulverized lyophilized Lemongrass powder, adding 200ml of 95% ethanol solution, and magnetically stirring at room temperature and 50rpm for 5h to obtain Lemongrass slurry.
And placing the obtained lemongrass serous fluid in an ice bath, carrying out ultrasonic treatment by an ultrasonic cell disruptor, wherein the ultrasonic power is 260W, the ultrasonic time is 20min, the working interval is 1s, and the ultrasonic working time is 10min, centrifuging the serous fluid at 10000rpm for 15min after the ultrasonic treatment is finished, and filtering by a 100-mesh filter screen to obtain a filtrate.
And carrying out reduced pressure rotary distillation on the filtrate under the condition of constant-temperature water bath, wherein the rotary evaporation temperature is 65 ℃, the rotary evaporation speed is 150rpm, the rotary evaporation time is 27min, and the vacuum degree is 0.09MPa, and obtaining the lemongrass essential oil after the rotary evaporation is finished.
Comparative example 1
Cutting fresh lemongrass, freezing at-80 deg.C for 1d, and vacuum freeze-drying at-40 deg.C for 2d to obtain freeze-dried lemongrass material;
weighing 10g of pulverized lyophilized Lemongrass powder, adding 200ml of distilled water, and magnetically stirring at room temperature and 100rpm for 5h to obtain Lemongrass slurry.
And (4) putting the obtained lemongrass pulp into a steam distillation device for steam distillation, and collecting oil-water mixed fraction.
Mixing the obtained oil-water mixed fraction with petroleum ether with a boiling range of 30-60 ℃, separating an oil layer, performing reduced pressure rotary distillation on the oil layer under the condition of a constant-temperature water bath, wherein the rotary evaporation temperature is 60 ℃, the rotary evaporation speed is 100rpm, the rotary evaporation time is 25min, the vacuum degree is 0.08MPa, and obtaining the lemongrass essential oil after the rotary evaporation is finished.
Comparative example 2
Cutting fresh lemongrass, freezing at-80 deg.C for 1d, and vacuum freeze-drying at-40 deg.C for 2d to obtain freeze-dried lemongrass material;
weighing 10g of pulverized lyophilized Lemongrass powder, adding 200ml of distilled water, and magnetically stirring at room temperature and 100rpm for 5h to obtain Lemongrass slurry.
And (3) carrying out ultrasonic auxiliary crushing treatment on the obtained lemongrass pulp, wherein the ultrasonic power is 180W, the ultrasonic time is 20min, the working interval is 2s, and the ultrasonic working time is 10min, centrifuging the pulp for 10min at 9000rpm after the ultrasonic treatment is finished, and filtering by a 110-mesh filter screen to obtain filtrate.
And (4) putting the filtrate into a steam distillation device for steam distillation, and collecting oil-water mixed fraction.
Mixing the obtained oil-water mixed fraction with petroleum ether with a boiling range of 30-60 ℃, separating an oil layer, performing reduced pressure rotary distillation on the oil layer under the condition of a constant-temperature water bath, wherein the rotary evaporation temperature is 60 ℃, the rotary evaporation speed is 100rpm, the rotary evaporation time is 25min, the vacuum degree is 0.08MPa, and obtaining the lemongrass essential oil after the rotary evaporation is finished.
Experimental example 1 calculation of extraction ratio of essential oil
The method comprises the following steps: the lemongrass essential oil obtained by the methods of example 1, example 2, example 3, comparative example 1 and comparative example 2 is subjected to quality measurement, and the extraction rate of the essential oil is calculated by the following formula:
the extraction rate of essential oil is (mass of essential oil/mass of lemongrass raw material) × 100%.
The results are shown in table 1 below:
TABLE 1 extraction of essential oils
| Example 1 | Example 2 | Example 3 | Comparative example 1 | Comparative example 2 | |
| Extraction rate of essential oil | 6.77％ | 6.73％ | 6.47％ | 3.90％ | 5.22％ |
Experimental example 2 calculation of citral content in essential oil
The principle is as follows: the maximum absorption wavelength of the standard is measured, a standard curve is drawn according to the light absorption value under the maximum absorption wavelength, then the light absorption value under the corresponding wavelength is measured for the extracted essential oil, and the content ratio of the main component citral in the essential oil is calculated according to the standard curve.
The method comprises the following steps: by measuring the maximum absorption wavelength of the standard substance, 50, 100, 150, 200 and 250 mu L of citral standard substance are dissolved in 10mL of absolute ethanol, and a standard curve is drawn according to the absorbance value at the maximum absorption wavelength.
The maximum absorption wavelength of the citral standard is 390nm, and the standard equation is OD390 ═ 0.0342X-0.033(X ═ citral content μ L/mL). Then, the essential oil extracted in the above examples and comparative examples is dissolved in 10mL of absolute ethyl alcohol, the light absorption value is measured under 390nm, and the content ratio of the main component citral in the essential oil is calculated according to a standard curve, and the calculation formula is as follows:
the citral content (%) (% citral content X10 × 0.899)/essential oil taken in mass (ρ citral ═ 0.899mg/μ L).
The results are shown in table 2 below:
TABLE 2 citral content in essential oils
| Example 1 | Example 2 | Example 3 | Comparative example 1 | Comparative example 2 | |
| Quality of essential oil | 0.060g | 0.100g | 0.100g | 0.060g | 0.060g |
| OD390 | 0.086 | 0.171 | 0.167 | 0.065 | 0.076 |
| Citral content | 52.05％ | 53.65％ | 52.66％ | 43.13％ | 47.68％ |
According to the embodiments, the preparation method of the lemongrass essential oil provided by the invention can avoid the loss of active substances of the lemongrass essential oil and the hydrolysis and emulsification phenomena possibly occurring in the extraction method, and can effectively improve the stability and purity of the essential oil; the invention takes ethanol as an extracting agent, can avoid the problem of residual toxicity of the solvent, and can continue to recycle, thereby improving the economic benefit of production; the preparation method can quickly and efficiently separate the essential oil, and the low-temperature condition in the ultrasonic process can relieve the heat generation phenomenon in the ultrasonic treatment process, thereby playing a certain protection role on active substances. The preparation method provided by the invention improves the extraction efficiency of the lemongrass essential oil, the final extraction rate of the lemongrass essential oil can reach 6.7% under the condition of the preparation method, the content of the main active ingredient, namely citral, can reach 52%, and the extraction efficiency and the essential oil yield are effectively improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The preparation method of the lemongrass essential oil is characterized by comprising the following steps:
s1, sequentially freezing, freeze-drying and crushing the lemongrass to obtain freeze-dried lemongrass powder;
s2, mixing the freeze-dried lemongrass powder with an alcohol solution, and extracting to obtain lemongrass pulp;
s3, sequentially carrying out ultrasonic treatment, centrifugation and filtration on the lemongrass pulp to obtain filtrate;
s4, distilling the obtained filtrate to obtain the lemongrass essential oil.
2. The method for preparing lemongrass essential oil according to claim 1, wherein the freezing temperature in step S1 is-70 to-90 ℃ and the time is 0.5 to 1.5 days.
3. The method for preparing lemongrass essential oil according to claim 1 or 2, wherein the freeze drying in step S1 is vacuum freeze drying, the temperature of the vacuum freeze drying is-30 to-50 ℃, and the time of the vacuum freeze drying is 1.5 to 2.5 days.
4. The method for preparing lemongrass essential oil according to claim 3, wherein the alcohol solution in step S2 is an ethanol solution, and the volume concentration of the ethanol solution is 90-99%;
the mass volume ratio of the freeze-dried citronella grass powder to the ethanol solution is 1 g: 20-30 ml.
5. The method for preparing lemongrass essential oil according to claim 4, wherein the extraction process in the step S2 is accompanied by stirring, and the rotation speed of the stirring is 50-150 rpm.
6. The method for preparing lemongrass essential oil according to claim 5, wherein the ultrasonic treatment is intermittently performed in step S3, the power of the ultrasonic treatment is 240-280W, the total time of the ultrasonic treatment is 20-30 min, and the interval time between the intermittent ultrasonic treatment is 1-3S.
7. The method for preparing lemongrass essential oil according to claim 6, wherein the temperature of the ultrasonic treatment in the step S3 is-5 to 5 ℃.
8. The method for preparing lemongrass essential oil according to claim 1 or 7, wherein the centrifugation in step S3 is performed at 8000-10000 rpm for 10-15 min.
9. The method for preparing lemongrass essential oil according to claim 8, wherein the filtering mesh number in step S3 is 80-120 meshes.
10. The method for preparing lemongrass essential oil according to claim 9, wherein the distillation in step S4 is reduced pressure rotary distillation, the temperature of the reduced pressure rotary distillation is 60-65 ℃, the rotation speed of the reduced pressure rotary distillation is 50-150 rpm, the time of the reduced pressure rotary distillation is 20-30 min, and the vacuum degree of the reduced pressure rotary distillation is 0.05-0.1 MPa.
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| CN115141682A (en) * | 2022-06-21 | 2022-10-04 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method and application of essential oil in fructus alpiniae oxyphyllae |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104911031A (en) * | 2015-06-04 | 2015-09-16 | 海南科技职业学院 | Extraction method for citronella oil antibacterial substances |
| CN106221926A (en) * | 2016-08-26 | 2016-12-14 | 广西灏源盛世生物科技有限公司 | A kind of extracting method of Herba Cymbopogonis Citrari quintessence oil |
| CN106244326A (en) * | 2016-08-26 | 2016-12-21 | 广西灏源盛世生物科技有限公司 | A kind of Herba Cymbopogonis Citrari extraction of essential oil processing technique |
| CN106336950A (en) * | 2016-08-31 | 2017-01-18 | 防城港市绿华源农林科技有限公司 | Method for extracting essential oil from lemongrass |
| CN109601559A (en) * | 2019-01-07 | 2019-04-12 | 贵州省烟草公司遵义市公司 | It is a kind of prevent and treat phytophthora mother chrysanthemum essential oil and its application |
| CN111196949A (en) * | 2018-11-18 | 2020-05-26 | 南京艾蒙利特生物科技有限公司 | Preparation method of camphor essential oil |
-
2022
- 2022-01-19 CN CN202210062056.4A patent/CN114381335A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104911031A (en) * | 2015-06-04 | 2015-09-16 | 海南科技职业学院 | Extraction method for citronella oil antibacterial substances |
| CN106221926A (en) * | 2016-08-26 | 2016-12-14 | 广西灏源盛世生物科技有限公司 | A kind of extracting method of Herba Cymbopogonis Citrari quintessence oil |
| CN106244326A (en) * | 2016-08-26 | 2016-12-21 | 广西灏源盛世生物科技有限公司 | A kind of Herba Cymbopogonis Citrari extraction of essential oil processing technique |
| CN106336950A (en) * | 2016-08-31 | 2017-01-18 | 防城港市绿华源农林科技有限公司 | Method for extracting essential oil from lemongrass |
| CN111196949A (en) * | 2018-11-18 | 2020-05-26 | 南京艾蒙利特生物科技有限公司 | Preparation method of camphor essential oil |
| CN109601559A (en) * | 2019-01-07 | 2019-04-12 | 贵州省烟草公司遵义市公司 | It is a kind of prevent and treat phytophthora mother chrysanthemum essential oil and its application |
Non-Patent Citations (1)
| Title |
|---|
| 刁水华: "香茅提取物微胶囊的制备及锦纶针织物的驱蚊整理" * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115141682A (en) * | 2022-06-21 | 2022-10-04 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method and application of essential oil in fructus alpiniae oxyphyllae |
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