CN114377183A - Sterilization filtering method of air filter - Google Patents
Sterilization filtering method of air filter Download PDFInfo
- Publication number
- CN114377183A CN114377183A CN202111606572.0A CN202111606572A CN114377183A CN 114377183 A CN114377183 A CN 114377183A CN 202111606572 A CN202111606572 A CN 202111606572A CN 114377183 A CN114377183 A CN 114377183A
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- air
- filter
- sampling
- filter material
- particles
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- 238000001914 filtration Methods 0.000 title claims abstract description 29
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 29
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims description 11
- 239000002245 particle Substances 0.000 claims abstract description 29
- 239000000463 material Substances 0.000 claims abstract description 28
- 239000000428 dust Substances 0.000 claims abstract description 15
- 230000000694 effects Effects 0.000 claims abstract description 10
- 238000005070 sampling Methods 0.000 claims description 24
- 244000005700 microbiome Species 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 16
- 238000011144 upstream manufacturing Methods 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 241001052560 Thallis Species 0.000 claims description 9
- 230000005855 radiation Effects 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 241000191967 Staphylococcus aureus Species 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 102000016943 Muramidase Human genes 0.000 claims description 5
- 108010014251 Muramidase Proteins 0.000 claims description 5
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 5
- 229960000274 lysozyme Drugs 0.000 claims description 5
- 235000010335 lysozyme Nutrition 0.000 claims description 5
- 239000004325 lysozyme Substances 0.000 claims description 5
- 230000005653 Brownian motion process Effects 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000000443 aerosol Substances 0.000 claims description 3
- 238000005537 brownian motion Methods 0.000 claims description 3
- 238000009792 diffusion process Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 230000005484 gravity Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000007246 mechanism Effects 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- MNLRQHMNZILYPY-MDMHTWEWSA-N N-acetyl-alpha-D-muramic acid Chemical compound OC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@@H]1NC(C)=O MNLRQHMNZILYPY-MDMHTWEWSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000012569 microbial contaminant Substances 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D50/00—Combinations of methods or devices for separating particles from gases or vapours
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/10—Apparatus features
- A61L2209/14—Filtering means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2279/00—Filters adapted for separating dispersed particles from gases or vapours specially modified for specific uses
- B01D2279/65—Filters adapted for separating dispersed particles from gases or vapours specially modified for specific uses for the sterilisation of air
Abstract
The invention provides a sterilization and filtration method of an air filter, which comprises the following steps: s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter; s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle; and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state. The invention provides a sterilization and filtration method of an air filter, which can sterilize while filtering, has wide application range and is easy to manufacture.
Description
Technical Field
The invention relates to the technical field of air filtration, in particular to a sterilization and filtration method of an air filter.
Background
The sterilizing filter is a new product which is emerging in recent years, and is applied to biological clean rooms, microorganism laboratories, sterile rooms of hospitals, pharmaceutical factories (GMP engineering), food factories, wide central air conditioners, household air conditioners and the like. It combines a biocide or other sterilization method with a conventional filter to remove microbial contaminants.
The sterilization and filtration performance of the air filter in China at present is not perfect enough.
Disclosure of Invention
The present invention is directed to a sterilization and filtration method for an air filter to solve the above problems.
In order to achieve the purpose, the invention provides the following technical scheme: a sterilization and filtration method of an air filter comprises the following steps:
s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter;
s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle;
and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state.
As a modification of the present invention, in step S10, the sterilizing and filtering mechanism of the air filter includes: the interception effect of the filter material fibers on the particles, the mutual inertial collision of the particles in the air flow, the Brownian motion diffusion collision of small particles, the sedimentation of the particles under the self gravity and the friction and electrostatic adsorption among the particles.
As a modification of the present invention, step S20 specifically includes:
s21, selecting escherichia coli and staphylococcus aureus as detection thalli;
s22, establishing a far infrared radiation heating filter experiment platform, and keeping the temperature of the filter at 150 ℃;
and S23, placing a dust particle counter at the air outlet of the filter, and counting the dust particles in the air filtered by the filter.
As a modification of the present invention, step S30 specifically includes:
s31, selecting escherichia coli and staphylococcus aureus as detection thalli;
s32, adopting a lysozyme filter material as a test main body;
s33, establishing a test board for the sterilizing effect of the filter material, and detecting that the thalli are sprayed to the filter material in an aerosol mode by a sprayer;
s34, respectively arranging a microorganism sampler at the upstream and the downstream of the filter material, counting the sampling results of the two microorganism samplers by adopting a scanning electron microscope, and calculating the bacterial filtering effect according to the counting results.
As a modification of the present invention, the specific operation procedure of step S33 is:
s331, wiping the inside and outside of the microorganism sampler by using absorbent cotton dipped with 75% alcohol, igniting the residual alcohol on flame, and achieving the aim of disinfection after the residual alcohol is completely combusted;
s332, mounting the filter material to be tested on a clamp, placing a sealing gasket, and starting an air compressor to compress the filter material;
s333, connecting a vacuum pump to a glass bottle, and continuously introducing clean air into the bottle for 1 minute;
s334, starting a pressurizing fan, and adjusting the frequency to enable the air volume to reach 30L/min;
s335, putting the bacterial suspension into a liquid spray bottle, connecting a sprayer, starting an air pump, continuously fermenting bacteria in a glass bottle, and simultaneously introducing clean air into an upstream buffer bottle, wherein the fermentation flow is 0.15 ml/min;
s336, connecting the microorganism sampler with the culture dish to an upstream microorganism sampling port, switching on a power supply, sampling for 5 minutes in the upstream with a sampling flow of 25L/min, taking out the culture dish after sampling is finished, and covering a culture dish cover;
s337, fermenting bacteria in the center, filtering the bacteria by filter paper, continuously performing for 20min, stopping fermenting bacteria by the fan, and continuously introducing clean air into the bottle for 10 min;
s338, sampling 5min at the downstream by using a microorganism sampler when bacteria are grown in the clamp, wherein the sampling flow is 25L/min;
and S339, after sampling, closing the air compressor, stopping pressurizing the fan, and putting the filter material in the clamp into a sealing bag.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
A sterilization and filtration method of an air filter comprises the following steps:
s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter;
s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle;
and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state.
As an embodiment of the present invention, in step S10, the sterilizing and filtering mechanism of the air filter includes: the interception effect of the filter material fibers on the particles, the mutual inertial collision of the particles in the air flow, the Brownian motion diffusion collision of small particles, the sedimentation of the particles under the self gravity and the friction and electrostatic adsorption among the particles.
As an embodiment of the present invention, step S20 specifically includes:
s21, selecting escherichia coli and staphylococcus aureus as detection thalli;
s22, establishing a far infrared radiation heating filter experiment platform, and keeping the temperature of the filter at 150 ℃;
and S23, placing a dust particle counter at the air outlet of the filter, and counting the dust particles in the air filtered by the filter.
As an embodiment of the present invention, step S30 specifically includes:
s31, selecting escherichia coli and staphylococcus aureus as detection thalli;
s32, adopting a lysozyme filter material as a test main body;
s33, establishing a test board for the sterilizing effect of the filter material, and detecting that the thalli are sprayed to the filter material in an aerosol mode by a sprayer;
s34, respectively arranging a microorganism sampler at the upstream and the downstream of the filter material, counting the sampling results of the two microorganism samplers by adopting a scanning electron microscope, and calculating the bacterial filtering effect according to the counting results.
As an embodiment of the present invention, the specific operation procedure of step S33 is as follows:
s331, wiping the inside and outside of the microorganism sampler by using absorbent cotton dipped with 75% alcohol, igniting the residual alcohol on flame, and achieving the aim of disinfection after the residual alcohol is completely combusted;
s332, mounting the filter material to be tested on a clamp, placing a sealing gasket, and starting an air compressor to compress the filter material;
s333, connecting a vacuum pump to a glass bottle, and continuously introducing clean air into the bottle for 1 minute;
s334, starting a pressurizing fan, and adjusting the frequency to enable the air volume to reach 30L/min;
s335, putting the bacterial suspension into a liquid spray bottle, connecting a sprayer, starting an air pump, continuously fermenting bacteria in a glass bottle, and simultaneously introducing clean air into an upstream buffer bottle, wherein the fermentation flow is 0.15 ml/min;
s336, connecting the microorganism sampler with the culture dish to an upstream microorganism sampling port, switching on a power supply, sampling for 5 minutes in the upstream with a sampling flow of 25L/min, taking out the culture dish after sampling is finished, and covering a culture dish cover;
s337, fermenting bacteria in the center, filtering the bacteria by filter paper, continuously performing for 20min, stopping fermenting bacteria by the fan, and continuously introducing clean air into the bottle for 10 min;
s338, sampling 5min at the downstream by using a microorganism sampler when bacteria are grown in the clamp, wherein the sampling flow is 25L/min;
and S339, after sampling, closing the air compressor, stopping pressurizing the fan, and putting the filter material in the clamp into a sealing bag.
The working principle and the beneficial effects of the technical scheme are as follows:
the experimental system of the far infrared radiation heating filter utilizes a far infrared radiation plate as a heat source to heat the high-efficiency filter in the air. The far infrared radiation plate is positioned at the upwind side of the high-efficiency filter, and when the temperature of the high-efficiency filter rises to a certain value and is kept for a corresponding time, microorganisms caught by the high-efficiency filter can be killed. The distance between the radiation plate and the filter is 150 mm. The far infrared radiation heating temperature can reach 250-350 ℃, the heating and sterilizing process is repeated, and the high temperature resistant filter can be repeatedly used as long as the final resistance and dust holding capacity during replacement are not reached. And through a plurality of times of experimental detection, the filtering efficiency of the high-temperature resistant filter before and after heating does not change obviously, so that the service life of the filter is not influenced.
The lysozyme has the function of catalyzing and hydrolyzing the connection between-l, 4 glycosidic bonds between the N-acetylmuramic acid and the N-acetylglucosamine, destroying the peptidoglycan scaffold, and leading cells to swell and crack under the action of internal osmotic pressure to cause bacterial lysis. Coli is lysed by lysozyme to produce cell wall rupture until death. The method for observing by using a scanning electron microscope has obvious advantages, and the growth and death conditions of bacteria can be intuitively observed. Under a scanning electron microscope, the organelles of the cells, viruses, can be seen.
The invention provides a sterilization and filtration method of an air filter, which can sterilize while filtering, has wide application range and is easy to manufacture.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (5)
1. A sterilization and filtration method of an air filter is characterized by comprising the following steps:
s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter;
s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle;
and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state.
2. The method as claimed in claim 1, wherein in step S10, the air filter sterilization and filtration mechanism comprises: the interception effect of the filter material fibers on the particles, the mutual inertial collision of the particles in the air flow, the Brownian motion diffusion collision of small particles, the sedimentation of the particles under the self gravity and the friction and electrostatic adsorption among the particles.
3. The method as claimed in claim 1, wherein the step S20 specifically includes:
s21, selecting escherichia coli and staphylococcus aureus as detection thalli;
s22, establishing a far infrared radiation heating filter experiment platform, and keeping the temperature of the filter at 150 ℃;
and S23, placing a dust particle counter at the air outlet of the filter, and counting the dust particles in the air filtered by the filter.
4. The method as claimed in claim 1, wherein the step S30 specifically includes:
s31, selecting escherichia coli and staphylococcus aureus as detection thalli;
s32, adopting a lysozyme filter material as a test main body;
s33, establishing a test board for the sterilizing effect of the filter material, and detecting that the thalli are sprayed to the filter material in an aerosol mode by a sprayer;
s34, respectively arranging a microorganism sampler at the upstream and the downstream of the filter material, counting the sampling results of the two microorganism samplers by adopting a scanning electron microscope, and calculating the bacterial filtering effect according to the counting results.
5. The sterilization and filtration method of an air filter according to claim 4, wherein the specific operation process of step S33 is as follows:
s331, wiping the inside and outside of the microorganism sampler by using absorbent cotton dipped with 75% alcohol, igniting the residual alcohol on flame, and achieving the aim of disinfection after the residual alcohol is completely combusted;
s332, mounting the filter material to be tested on a clamp, placing a sealing gasket, and starting an air compressor to compress the filter material;
s333, connecting a vacuum pump to a glass bottle, and continuously introducing clean air into the bottle for 1 minute;
s334, starting a pressurizing fan, and adjusting the frequency to enable the air volume to reach 30L/min;
s335, putting the bacterial suspension into a liquid spray bottle, connecting a sprayer, starting an air pump, continuously fermenting bacteria in a glass bottle, and simultaneously introducing clean air into an upstream buffer bottle, wherein the fermentation flow is 0.15 ml/min;
s336, connecting the microorganism sampler with the culture dish to an upstream microorganism sampling port, switching on a power supply, sampling for 5 minutes in the upstream with a sampling flow of 25L/min, taking out the culture dish after sampling is finished, and covering a culture dish cover;
s337, fermenting bacteria in the center, filtering the bacteria by filter paper, continuously performing for 20min, stopping fermenting bacteria by the fan, and continuously introducing clean air into the bottle for 10 min;
s338, sampling 5min at the downstream by using a microorganism sampler when bacteria are grown in the clamp, wherein the sampling flow is 25L/min;
and S339, after sampling, closing the air compressor, stopping pressurizing the fan, and putting the filter material in the clamp into a sealing bag.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202111606572.0A CN114377183A (en) | 2021-12-26 | 2021-12-26 | Sterilization filtering method of air filter |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202111606572.0A CN114377183A (en) | 2021-12-26 | 2021-12-26 | Sterilization filtering method of air filter |
Publications (1)
Publication Number | Publication Date |
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CN114377183A true CN114377183A (en) | 2022-04-22 |
Family
ID=81197269
Family Applications (1)
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CN202111606572.0A Pending CN114377183A (en) | 2021-12-26 | 2021-12-26 | Sterilization filtering method of air filter |
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08144199A (en) * | 1994-11-17 | 1996-06-04 | Nippon Glass Fiber Co Ltd | Filter paper for antimicrobial air filter and its production |
CN101995372A (en) * | 2010-10-19 | 2011-03-30 | 北京市劳动保护科学研究所 | Air filtering material tester |
CN201793572U (en) * | 2010-09-03 | 2011-04-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Safety cabinet detection system for biologically detecting high efficiency particulate air filter |
CN106999953A (en) * | 2014-12-12 | 2017-08-01 | 里兰斯坦福初级大学理事会 | The air cleaner captured for high efficiency PM2.5 |
CN107389526A (en) * | 2017-07-24 | 2017-11-24 | 上海市建筑科学研究院(集团)有限公司 | A kind of air filter efficiency evaluation method |
CN107616063A (en) * | 2017-10-21 | 2018-01-23 | 郴州市芝草农业科技开发有限公司 | Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation |
CN207478175U (en) * | 2017-10-26 | 2018-06-12 | 佛山市顺德区阿波罗环保器材有限公司 | The air purifier of detectable filter element life |
KR20210091004A (en) * | 2020-01-13 | 2021-07-21 | 주식회사 볼트크리에이션 | Negative voltage air filter and fine dust filtering device using the filter |
CN214408583U (en) * | 2020-12-09 | 2021-10-15 | 天津城建大学 | Ventilation is with air cleaner microbiological filtration performance evaluation laboratory bench |
-
2021
- 2021-12-26 CN CN202111606572.0A patent/CN114377183A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08144199A (en) * | 1994-11-17 | 1996-06-04 | Nippon Glass Fiber Co Ltd | Filter paper for antimicrobial air filter and its production |
CN201793572U (en) * | 2010-09-03 | 2011-04-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Safety cabinet detection system for biologically detecting high efficiency particulate air filter |
CN101995372A (en) * | 2010-10-19 | 2011-03-30 | 北京市劳动保护科学研究所 | Air filtering material tester |
CN106999953A (en) * | 2014-12-12 | 2017-08-01 | 里兰斯坦福初级大学理事会 | The air cleaner captured for high efficiency PM2.5 |
CN107389526A (en) * | 2017-07-24 | 2017-11-24 | 上海市建筑科学研究院(集团)有限公司 | A kind of air filter efficiency evaluation method |
CN107616063A (en) * | 2017-10-21 | 2018-01-23 | 郴州市芝草农业科技开发有限公司 | Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation |
CN207478175U (en) * | 2017-10-26 | 2018-06-12 | 佛山市顺德区阿波罗环保器材有限公司 | The air purifier of detectable filter element life |
KR20210091004A (en) * | 2020-01-13 | 2021-07-21 | 주식회사 볼트크리에이션 | Negative voltage air filter and fine dust filtering device using the filter |
CN214408583U (en) * | 2020-12-09 | 2021-10-15 | 天津城建大学 | Ventilation is with air cleaner microbiological filtration performance evaluation laboratory bench |
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