CN114377183A - Sterilization filtering method of air filter - Google Patents

Sterilization filtering method of air filter Download PDF

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Publication number
CN114377183A
CN114377183A CN202111606572.0A CN202111606572A CN114377183A CN 114377183 A CN114377183 A CN 114377183A CN 202111606572 A CN202111606572 A CN 202111606572A CN 114377183 A CN114377183 A CN 114377183A
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CN
China
Prior art keywords
air
filter
sampling
filter material
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111606572.0A
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Chinese (zh)
Inventor
李成忠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yancheng Jude Machinery Parts Co ltd
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Yancheng Jude Machinery Parts Co ltd
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Publication date
Application filed by Yancheng Jude Machinery Parts Co ltd filed Critical Yancheng Jude Machinery Parts Co ltd
Priority to CN202111606572.0A priority Critical patent/CN114377183A/en
Publication of CN114377183A publication Critical patent/CN114377183A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00Disinfection, sterilisation or deodorisation of air
    • A61L9/16Disinfection, sterilisation or deodorisation of air using physical phenomena
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D50/00Combinations of methods or devices for separating particles from gases or vapours
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2209/00Aspects relating to disinfection, sterilisation or deodorisation of air
    • A61L2209/10Apparatus features
    • A61L2209/14Filtering means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2279/00Filters adapted for separating dispersed particles from gases or vapours specially modified for specific uses
    • B01D2279/65Filters adapted for separating dispersed particles from gases or vapours specially modified for specific uses for the sterilisation of air

Abstract

The invention provides a sterilization and filtration method of an air filter, which comprises the following steps: s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter; s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle; and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state. The invention provides a sterilization and filtration method of an air filter, which can sterilize while filtering, has wide application range and is easy to manufacture.

Description

Sterilization filtering method of air filter
Technical Field
The invention relates to the technical field of air filtration, in particular to a sterilization and filtration method of an air filter.
Background
The sterilizing filter is a new product which is emerging in recent years, and is applied to biological clean rooms, microorganism laboratories, sterile rooms of hospitals, pharmaceutical factories (GMP engineering), food factories, wide central air conditioners, household air conditioners and the like. It combines a biocide or other sterilization method with a conventional filter to remove microbial contaminants.
The sterilization and filtration performance of the air filter in China at present is not perfect enough.
Disclosure of Invention
The present invention is directed to a sterilization and filtration method for an air filter to solve the above problems.
In order to achieve the purpose, the invention provides the following technical scheme: a sterilization and filtration method of an air filter comprises the following steps:
s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter;
s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle;
and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state.
As a modification of the present invention, in step S10, the sterilizing and filtering mechanism of the air filter includes: the interception effect of the filter material fibers on the particles, the mutual inertial collision of the particles in the air flow, the Brownian motion diffusion collision of small particles, the sedimentation of the particles under the self gravity and the friction and electrostatic adsorption among the particles.
As a modification of the present invention, step S20 specifically includes:
s21, selecting escherichia coli and staphylococcus aureus as detection thalli;
s22, establishing a far infrared radiation heating filter experiment platform, and keeping the temperature of the filter at 150 ℃;
and S23, placing a dust particle counter at the air outlet of the filter, and counting the dust particles in the air filtered by the filter.
As a modification of the present invention, step S30 specifically includes:
s31, selecting escherichia coli and staphylococcus aureus as detection thalli;
s32, adopting a lysozyme filter material as a test main body;
s33, establishing a test board for the sterilizing effect of the filter material, and detecting that the thalli are sprayed to the filter material in an aerosol mode by a sprayer;
s34, respectively arranging a microorganism sampler at the upstream and the downstream of the filter material, counting the sampling results of the two microorganism samplers by adopting a scanning electron microscope, and calculating the bacterial filtering effect according to the counting results.
As a modification of the present invention, the specific operation procedure of step S33 is:
s331, wiping the inside and outside of the microorganism sampler by using absorbent cotton dipped with 75% alcohol, igniting the residual alcohol on flame, and achieving the aim of disinfection after the residual alcohol is completely combusted;
s332, mounting the filter material to be tested on a clamp, placing a sealing gasket, and starting an air compressor to compress the filter material;
s333, connecting a vacuum pump to a glass bottle, and continuously introducing clean air into the bottle for 1 minute;
s334, starting a pressurizing fan, and adjusting the frequency to enable the air volume to reach 30L/min;
s335, putting the bacterial suspension into a liquid spray bottle, connecting a sprayer, starting an air pump, continuously fermenting bacteria in a glass bottle, and simultaneously introducing clean air into an upstream buffer bottle, wherein the fermentation flow is 0.15 ml/min;
s336, connecting the microorganism sampler with the culture dish to an upstream microorganism sampling port, switching on a power supply, sampling for 5 minutes in the upstream with a sampling flow of 25L/min, taking out the culture dish after sampling is finished, and covering a culture dish cover;
s337, fermenting bacteria in the center, filtering the bacteria by filter paper, continuously performing for 20min, stopping fermenting bacteria by the fan, and continuously introducing clean air into the bottle for 10 min;
s338, sampling 5min at the downstream by using a microorganism sampler when bacteria are grown in the clamp, wherein the sampling flow is 25L/min;
and S339, after sampling, closing the air compressor, stopping pressurizing the fan, and putting the filter material in the clamp into a sealing bag.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
A sterilization and filtration method of an air filter comprises the following steps:
s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter;
s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle;
and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state.
As an embodiment of the present invention, in step S10, the sterilizing and filtering mechanism of the air filter includes: the interception effect of the filter material fibers on the particles, the mutual inertial collision of the particles in the air flow, the Brownian motion diffusion collision of small particles, the sedimentation of the particles under the self gravity and the friction and electrostatic adsorption among the particles.
As an embodiment of the present invention, step S20 specifically includes:
s21, selecting escherichia coli and staphylococcus aureus as detection thalli;
s22, establishing a far infrared radiation heating filter experiment platform, and keeping the temperature of the filter at 150 ℃;
and S23, placing a dust particle counter at the air outlet of the filter, and counting the dust particles in the air filtered by the filter.
As an embodiment of the present invention, step S30 specifically includes:
s31, selecting escherichia coli and staphylococcus aureus as detection thalli;
s32, adopting a lysozyme filter material as a test main body;
s33, establishing a test board for the sterilizing effect of the filter material, and detecting that the thalli are sprayed to the filter material in an aerosol mode by a sprayer;
s34, respectively arranging a microorganism sampler at the upstream and the downstream of the filter material, counting the sampling results of the two microorganism samplers by adopting a scanning electron microscope, and calculating the bacterial filtering effect according to the counting results.
As an embodiment of the present invention, the specific operation procedure of step S33 is as follows:
s331, wiping the inside and outside of the microorganism sampler by using absorbent cotton dipped with 75% alcohol, igniting the residual alcohol on flame, and achieving the aim of disinfection after the residual alcohol is completely combusted;
s332, mounting the filter material to be tested on a clamp, placing a sealing gasket, and starting an air compressor to compress the filter material;
s333, connecting a vacuum pump to a glass bottle, and continuously introducing clean air into the bottle for 1 minute;
s334, starting a pressurizing fan, and adjusting the frequency to enable the air volume to reach 30L/min;
s335, putting the bacterial suspension into a liquid spray bottle, connecting a sprayer, starting an air pump, continuously fermenting bacteria in a glass bottle, and simultaneously introducing clean air into an upstream buffer bottle, wherein the fermentation flow is 0.15 ml/min;
s336, connecting the microorganism sampler with the culture dish to an upstream microorganism sampling port, switching on a power supply, sampling for 5 minutes in the upstream with a sampling flow of 25L/min, taking out the culture dish after sampling is finished, and covering a culture dish cover;
s337, fermenting bacteria in the center, filtering the bacteria by filter paper, continuously performing for 20min, stopping fermenting bacteria by the fan, and continuously introducing clean air into the bottle for 10 min;
s338, sampling 5min at the downstream by using a microorganism sampler when bacteria are grown in the clamp, wherein the sampling flow is 25L/min;
and S339, after sampling, closing the air compressor, stopping pressurizing the fan, and putting the filter material in the clamp into a sealing bag.
The working principle and the beneficial effects of the technical scheme are as follows:
the experimental system of the far infrared radiation heating filter utilizes a far infrared radiation plate as a heat source to heat the high-efficiency filter in the air. The far infrared radiation plate is positioned at the upwind side of the high-efficiency filter, and when the temperature of the high-efficiency filter rises to a certain value and is kept for a corresponding time, microorganisms caught by the high-efficiency filter can be killed. The distance between the radiation plate and the filter is 150 mm. The far infrared radiation heating temperature can reach 250-350 ℃, the heating and sterilizing process is repeated, and the high temperature resistant filter can be repeatedly used as long as the final resistance and dust holding capacity during replacement are not reached. And through a plurality of times of experimental detection, the filtering efficiency of the high-temperature resistant filter before and after heating does not change obviously, so that the service life of the filter is not influenced.
The lysozyme has the function of catalyzing and hydrolyzing the connection between-l, 4 glycosidic bonds between the N-acetylmuramic acid and the N-acetylglucosamine, destroying the peptidoglycan scaffold, and leading cells to swell and crack under the action of internal osmotic pressure to cause bacterial lysis. Coli is lysed by lysozyme to produce cell wall rupture until death. The method for observing by using a scanning electron microscope has obvious advantages, and the growth and death conditions of bacteria can be intuitively observed. Under a scanning electron microscope, the organelles of the cells, viruses, can be seen.
The invention provides a sterilization and filtration method of an air filter, which can sterilize while filtering, has wide application range and is easy to manufacture.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims (5)

1. A sterilization and filtration method of an air filter is characterized by comprising the following steps:
s10, analyzing the filtering and sterilizing principle of the air filter based on the air filter;
s20, in a gas phase state, counting the content of dust particles in the filtered air by using a dust particle counter and adopting a statistical principle;
and S30, visually observing the sterilization effect of the filter material by using a scanning electron microscope and adopting a statistical principle in a gas phase state.
2. The method as claimed in claim 1, wherein in step S10, the air filter sterilization and filtration mechanism comprises: the interception effect of the filter material fibers on the particles, the mutual inertial collision of the particles in the air flow, the Brownian motion diffusion collision of small particles, the sedimentation of the particles under the self gravity and the friction and electrostatic adsorption among the particles.
3. The method as claimed in claim 1, wherein the step S20 specifically includes:
s21, selecting escherichia coli and staphylococcus aureus as detection thalli;
s22, establishing a far infrared radiation heating filter experiment platform, and keeping the temperature of the filter at 150 ℃;
and S23, placing a dust particle counter at the air outlet of the filter, and counting the dust particles in the air filtered by the filter.
4. The method as claimed in claim 1, wherein the step S30 specifically includes:
s31, selecting escherichia coli and staphylococcus aureus as detection thalli;
s32, adopting a lysozyme filter material as a test main body;
s33, establishing a test board for the sterilizing effect of the filter material, and detecting that the thalli are sprayed to the filter material in an aerosol mode by a sprayer;
s34, respectively arranging a microorganism sampler at the upstream and the downstream of the filter material, counting the sampling results of the two microorganism samplers by adopting a scanning electron microscope, and calculating the bacterial filtering effect according to the counting results.
5. The sterilization and filtration method of an air filter according to claim 4, wherein the specific operation process of step S33 is as follows:
s331, wiping the inside and outside of the microorganism sampler by using absorbent cotton dipped with 75% alcohol, igniting the residual alcohol on flame, and achieving the aim of disinfection after the residual alcohol is completely combusted;
s332, mounting the filter material to be tested on a clamp, placing a sealing gasket, and starting an air compressor to compress the filter material;
s333, connecting a vacuum pump to a glass bottle, and continuously introducing clean air into the bottle for 1 minute;
s334, starting a pressurizing fan, and adjusting the frequency to enable the air volume to reach 30L/min;
s335, putting the bacterial suspension into a liquid spray bottle, connecting a sprayer, starting an air pump, continuously fermenting bacteria in a glass bottle, and simultaneously introducing clean air into an upstream buffer bottle, wherein the fermentation flow is 0.15 ml/min;
s336, connecting the microorganism sampler with the culture dish to an upstream microorganism sampling port, switching on a power supply, sampling for 5 minutes in the upstream with a sampling flow of 25L/min, taking out the culture dish after sampling is finished, and covering a culture dish cover;
s337, fermenting bacteria in the center, filtering the bacteria by filter paper, continuously performing for 20min, stopping fermenting bacteria by the fan, and continuously introducing clean air into the bottle for 10 min;
s338, sampling 5min at the downstream by using a microorganism sampler when bacteria are grown in the clamp, wherein the sampling flow is 25L/min;
and S339, after sampling, closing the air compressor, stopping pressurizing the fan, and putting the filter material in the clamp into a sealing bag.
CN202111606572.0A 2021-12-26 2021-12-26 Sterilization filtering method of air filter Pending CN114377183A (en)

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Application Number Priority Date Filing Date Title
CN202111606572.0A CN114377183A (en) 2021-12-26 2021-12-26 Sterilization filtering method of air filter

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111606572.0A CN114377183A (en) 2021-12-26 2021-12-26 Sterilization filtering method of air filter

Publications (1)

Publication Number Publication Date
CN114377183A true CN114377183A (en) 2022-04-22

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08144199A (en) * 1994-11-17 1996-06-04 Nippon Glass Fiber Co Ltd Filter paper for antimicrobial air filter and its production
CN101995372A (en) * 2010-10-19 2011-03-30 北京市劳动保护科学研究所 Air filtering material tester
CN201793572U (en) * 2010-09-03 2011-04-13 中国人民解放军军事医学科学院微生物流行病研究所 Safety cabinet detection system for biologically detecting high efficiency particulate air filter
CN106999953A (en) * 2014-12-12 2017-08-01 里兰斯坦福初级大学理事会 The air cleaner captured for high efficiency PM2.5
CN107389526A (en) * 2017-07-24 2017-11-24 上海市建筑科学研究院(集团)有限公司 A kind of air filter efficiency evaluation method
CN107616063A (en) * 2017-10-21 2018-01-23 郴州市芝草农业科技开发有限公司 Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation
CN207478175U (en) * 2017-10-26 2018-06-12 佛山市顺德区阿波罗环保器材有限公司 The air purifier of detectable filter element life
KR20210091004A (en) * 2020-01-13 2021-07-21 주식회사 볼트크리에이션 Negative voltage air filter and fine dust filtering device using the filter
CN214408583U (en) * 2020-12-09 2021-10-15 天津城建大学 Ventilation is with air cleaner microbiological filtration performance evaluation laboratory bench

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08144199A (en) * 1994-11-17 1996-06-04 Nippon Glass Fiber Co Ltd Filter paper for antimicrobial air filter and its production
CN201793572U (en) * 2010-09-03 2011-04-13 中国人民解放军军事医学科学院微生物流行病研究所 Safety cabinet detection system for biologically detecting high efficiency particulate air filter
CN101995372A (en) * 2010-10-19 2011-03-30 北京市劳动保护科学研究所 Air filtering material tester
CN106999953A (en) * 2014-12-12 2017-08-01 里兰斯坦福初级大学理事会 The air cleaner captured for high efficiency PM2.5
CN107389526A (en) * 2017-07-24 2017-11-24 上海市建筑科学研究院(集团)有限公司 A kind of air filter efficiency evaluation method
CN107616063A (en) * 2017-10-21 2018-01-23 郴州市芝草农业科技开发有限公司 Pleurotus eryngii bottle plants the comprehensive sterilization of factorial praluction inoculation
CN207478175U (en) * 2017-10-26 2018-06-12 佛山市顺德区阿波罗环保器材有限公司 The air purifier of detectable filter element life
KR20210091004A (en) * 2020-01-13 2021-07-21 주식회사 볼트크리에이션 Negative voltage air filter and fine dust filtering device using the filter
CN214408583U (en) * 2020-12-09 2021-10-15 天津城建大学 Ventilation is with air cleaner microbiological filtration performance evaluation laboratory bench

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Application publication date: 20220422

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