CN114371164A - Pb based on gold aggregation induced by tetramer DNA dye2+And Tl+Visual detection method - Google Patents
Pb based on gold aggregation induced by tetramer DNA dye2+And Tl+Visual detection method Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 230000002776 aggregation Effects 0.000 title claims abstract description 14
- 238000004220 aggregation Methods 0.000 title claims abstract description 14
- 230000000007 visual effect Effects 0.000 title claims abstract description 14
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 7
- 239000010931 gold Substances 0.000 title claims abstract description 7
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 17
- 230000008859 change Effects 0.000 claims abstract description 7
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 7
- 238000004458 analytical method Methods 0.000 claims abstract description 3
- 229910001385 heavy metal Inorganic materials 0.000 claims description 3
- 230000001939 inductive effect Effects 0.000 claims description 3
- 239000000975 dye Substances 0.000 claims 10
- 239000002105 nanoparticle Substances 0.000 claims 2
- RLQYRXCUPVKSAW-UHFFFAOYSA-M 2,3,9,10-tetramethoxy-5,6-dihydroisoquinolino[2,1-b]isoquinolin-7-ium;chloride Chemical compound [Cl-].COC1=C(OC)C=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=C1 RLQYRXCUPVKSAW-UHFFFAOYSA-M 0.000 claims 1
- 239000000980 acid dye Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- RIDQRIPSFYHEGL-UHFFFAOYSA-N fibrauretin Natural products CC12CC=C3C(=O)OC(CC3(C)C1C(=O)C=CC2=O)c4cocc4 RIDQRIPSFYHEGL-UHFFFAOYSA-N 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 238000011002 quantification Methods 0.000 claims 1
- ACOJCCLIDPZYJC-UHFFFAOYSA-M thiazole orange Chemical compound CC1=CC=C(S([O-])(=O)=O)C=C1.C1=CC=C2C(C=C3N(C4=CC=CC=C4S3)C)=CC=[N+](C)C2=C1 ACOJCCLIDPZYJC-UHFFFAOYSA-M 0.000 claims 1
- JADVWWSKYZXRGX-UHFFFAOYSA-M thioflavine T Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C1=[N+](C)C2=CC=C(C)C=C2S1 JADVWWSKYZXRGX-UHFFFAOYSA-M 0.000 claims 1
- 108020004414 DNA Proteins 0.000 abstract description 15
- 239000003245 coal Substances 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 108091081406 G-quadruplex Proteins 0.000 abstract description 2
- 230000005591 charge neutralization Effects 0.000 abstract description 2
- 238000011156 evaluation Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 238000002791 soaking Methods 0.000 abstract description 2
- 238000000673 graphite furnace atomic absorption spectrometry Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 10
- 239000000243 solution Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000012764 semi-quantitative analysis Methods 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 108091023037 Aptamer Proteins 0.000 description 2
- 229910052745 lead Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 206010043275 Teratogenicity Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- 238000001636 atomic emission spectroscopy Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 231100000211 teratogenicity Toxicity 0.000 description 1
- 229910052716 thallium Inorganic materials 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000003403 water pollutant Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
Pb based on gold aggregation induced by tetramer DNA dye2+And Tl+‑The visual detection method mainly utilizes the charge neutralization effect between the tetramer DNA dye and the AuNPs with negative charges to rapidly induce the aggregation of the AuNPs. Because the binding capacity of the tetrameric DNA to certain metal ions is higher than that of the tetrameric DNA dye, the dye can be replaced by the metal ions and released from the cavity of the G-quadruplex, the AuNPs are induced to aggregate, and the solution is changed from red to deep blue. By observing the change of the solution color, the method realizes the adjustment of Pb2+And Tl+Semi-quantitative detection of (3); analyzing RGB value of color by using smart phone to realize Pb2+And Tl+The detection limit of the quantitative detection of (3) can reach 3.3 nM. Meanwhile, the analysis result of the method on the sample is well matched with the GF-AAS measured value or the reference value. In addition, the sensing system has also been successfully used for children's playHas Pb in2+Tl in safety evaluation and coal mine rainwater soaking process+And (4) evaluating leakage. The method has the advantages of simple operation, high sensitivity, strong anti-interference capability and the like, and can be used for treating Pb in the environment2+And Tl+The detection has practical application value.
Description
Technical Field
The invention relates to Pb based on gold aggregation induced by tetramer DNA dye2+And Tl+A visual detection method, establishes the unmarked colorimetric sensing platform of AuNPs for Pb2+And Tl+Visual detection is carried out, and the method belongs to the technical field of environmental analysis.
Background
Pb and Tl are two adjacent heavy metal elements in the periodic table, Pb2+As a toxic and harmful water pollutant, the accumulation in the body can damage the intelligence development of children; tl+The compound has mutagenicity, carcinogenicity and teratogenicity, and can enter human bodies when being taken acutely or contacted chronically, so that the health of human bodies is greatly threatened. Traditional detection of Pb2+And Tl+The methods include atomic absorption spectroscopy, Inductively Coupled Plasma (ICP) -atomic emission spectroscopy or ICP-mass spectroscopy, and the like, and the analysis methods are expensive large instruments and difficult to realize field detection. The visual detection technology can realize semi-quantitative analysis of the object to be detected only by observing color change through naked eyes, and development of a data acquisition and processing system of the smart phone provides a new development direction for visual field detection. Therefore, the visual sensing technology is promising in the field detection of heavy metal ions in the environmental water sample.
In recent years, research has shown that Pb2+And Tl+Plasma is capable of specifically inducing G-rich sequence DNA to form tetrameric structures (Trend anal. chem., 2020,132, 116060). Based on this property, many tetramer DNA-based methods for detecting Pb have been developed2+And Tl+The visual detection technology (J. Phys. chem. C, 2010, 114, 16329-16334; ACS Sens., 2015, 1, 137-143), but most of the methods realize the aggregation of AuNPs through the induction of charge shielding salts, the dynamic process is complex, and the color of the sample is changed continuously during measurement, so that the quantitative determination is difficult (Macromolecules, 2017, 50, 7333-7343). Therefore, the invention adopts G-quadruplex dye to induce the aggregation of AuNPs through charge neutralization, and combines the data acquisition and processing system of the smart phone to establish Pb with high sensitivity and good selectivity2+And Tl+Unmarked visual sensing technology.
Disclosure of Invention
The invention aims to neutralize the negative charge on the surfaces of AuNPs by using the tetrameric DNA dye with positive charge, thereby rapidly inducing the aggregation of the AuNPs. Due to Pb2+And Tl+Can compete with the tetramer DNA dye for the cavity site of the tetramer DNA, so the dye can be released, the aggregation of AuNPs can be regulated and controlled, and Pb can be realized2+And Tl+High sensitivity detection of. The technical scheme of the invention is as follows:
(1) adding a tetramer DNA solution (prepared by using Tris-HCl buffer solution with the pH value of 7.4) into a 96 micro-porous plate, immediately adding tetramer DNA dye and AuNPs, uniformly stirring, wherein the color of the solution is red, and a blank signal is measured;
(2) adding target metal ions or sample solutions with different concentrations and a probe chain tetramer DNA solution (prepared by a Tris-HCl buffer solution with the pH value of 7.4) into a 96 micro-porous plate, immediately adding a tetramer DNA dye and AuNPs, uniformly stirring, converting the color of the solution into blue, and determining a sample signal;
(3) the visual semi-quantitative analysis of the target metal ions is realized by comparing the color change of AuNPs when the target exists or not; the color of the solution is analyzed through an application program of the smart phone, RGB values of Red (Red), Green (Green) and Blue (Blue) are obtained, and rapid and accurate quantitative detection of metal ions is achieved.
Effects of the invention
Compared with the prior art, the invention has the following advantages:
(1) the color development effect is good, and compared with the traditional salt-induced aggregation, the solution aggregated by the method is dark and stable in color.
(2) High sensitivity, Pb2+And Tl+The detection limit can reach 3.3 nM.
(3) The selectivity and the anti-interference capability are strong, and the anti-interference multiple to 16 metal ions is within the range of 10-2500.
(4) The practicality is strong, need not large-scale precision instrument, only needs the smart mobile phone just can realize the real-time supervision of sample, has easy and simple to handle, low cost's advantage.
Detailed Description
Example 1
In 96-well plates, Pb was added at various concentrations2+And 20 nM aptamer chain T30695 (5'-GGGTGGGTGGGTGGGT-3') in Tris-HCl buffer solution with pH 7.4, incubating for 5 min in dark place, sequentially adding ThT (1.30 μ M) and AuNPs (4.0 nM) into the solution, and stirring. Standing for 10 min, and administering fructus Alpinae OxyphyllaeThe colorimetric result can be recorded by photographing with a mobile phone (HUAWEI, Honor V10), and then the RGB value of the color is analyzed by using a color sampling application program; the color change of AuNPs is compared with the color change of the AuNPs in the presence of the target object to realize the adjustment of Pb2+Visual semi-quantitative analysis; the method realizes the Pb control by analyzing the RGB value of the color of the sample2+Accurate quantitative detection.
Example 2
Tl was added at different concentrations to 96-well plates+And 20 nM aptamer strand PS2.M (5'-GTG GGT AGG GCG GGT TGG-3') in Tris-HCl buffer pH 7.4, incubated for 5 min in the dark, and then ThT (1.30. mu.M) and AuNPs (4.0 nM) were added to the solution sequentially and stirred well. Standing for 10 min, photographing with a smart phone (HUAWEI, Honor V10) to record colorimetric result, and analyzing RGB value of color with color sampling application program; by comparing the color change of AuNPs when the target exists, Tl is realized+Visual semi-quantitative analysis; tl is realized by analyzing the RGB value of the color of the sample+Accurate quantitative detection.
The Pb in the standard water sample, the toy and the paint is detected by adopting the embodiments 1 and 22+The concentration and the Tl in a standard water sample and various waste water are detected simultaneously+The concentration and the result are well matched with the measured value of the graphite furnace atomic absorption method or the reference value of the standard water sample, and the result is reliable. Meanwhile, the method is applied to Pb in the children toy2+Safety evaluation and Tl in coal+And (4) evaluating leakage. After the children toy is soaked in simulated saliva for 36 h, Pb in the saliva2+The content is not detected yet, which indicates that the batch of toys contains Pb2+The content meets the national standard; tl increases with the soaking time after adding rain water into the coal+The leakage amount of the coal is gradually increased and reaches a platform after 12 hours, and two kinds of coal Tl are inspected+The leakage amounts of (A) were 5.85 ng/g and 9.41 ng/g, respectively.
Claims (5)
1. Pb based on gold aggregation induced by tetramer DNA dye2+And Tl+VisualizationThe detection method is characterized by comprising the following aspects:
(1) the positively charged tetramer DNA dye can neutralize the negative charge on the surface of gold nanoparticles (AuNPs), thereby causing the rapid aggregation of gold nanoparticles to present a deep blue color;
(2) when the tetramer is added, the tetramer dye can induce the tetramer dye to form a tetramer structure, the dye is embedded into a quadruplex cavity, the interaction between the dye and AuNPs is avoided, and the AuNPs are in a dispersed state and show red;
(3) when there is Pb2+And Tl+When present, Pb2+/Tl+The fluorescent dye competes with a tetramer DNA dye for a cavity of a quadruplex, so that the dye directly acts with AuNPs, and the AuNPs rapidly aggregate to be blue;
(4) realizes the heavy metal Pb by comparing the color change of AuNPs when the target exists or not2+And Tl+Visual detection and analysis; the color of the solution is analyzed through an application program of the smart phone, RGB values of Red (Red), Green (Green) and Blue (Blue) are obtained, and rapid quantification of metal ions is achieved.
2. The method of claim 1, wherein the agent for inducing aggregation of AuNPs is G4-DNA dye, which is a kind including thioflavin T, thiazole orange, fibrauretin, etc., and the concentration thereof is 0.8-1.4 μ M.
3. The method according to claim 1, wherein the tetrameric DNA is used in a concentration of 5 to 30 nM.
4. The method of claim 1, wherein the G4-DNA dye is incubated with G4-DNA to form a quadruplex for a period of 0-10 min.
5. The method of claim 1, wherein the nucleic acid dye induces aggregation of the AuNPs for a period of 0 to 10 minutes.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008008709A2 (en) * | 2006-07-11 | 2008-01-17 | Leap Bioscience Corporation | Method of selective protein enrichment and associated applications |
| CN103792229A (en) * | 2014-01-16 | 2014-05-14 | 中国科学院化学研究所 | Detection method for lead ion concentration and kit |
| CN110568175A (en) * | 2019-09-06 | 2019-12-13 | 成都理工大学 | Method for detecting DNA (deoxyribonucleic acid) based on rapid aggregation of nanogold induced by nucleic acid dye |
| CN113237856A (en) * | 2021-04-29 | 2021-08-10 | 中国人民解放军军事科学院军事医学研究院 | Aptamer-based detection Tl+Fluorescent probe of |
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2021
- 2021-12-08 CN CN202111489202.3A patent/CN114371164A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| WO2008008709A2 (en) * | 2006-07-11 | 2008-01-17 | Leap Bioscience Corporation | Method of selective protein enrichment and associated applications |
| CN103792229A (en) * | 2014-01-16 | 2014-05-14 | 中国科学院化学研究所 | Detection method for lead ion concentration and kit |
| CN110568175A (en) * | 2019-09-06 | 2019-12-13 | 成都理工大学 | Method for detecting DNA (deoxyribonucleic acid) based on rapid aggregation of nanogold induced by nucleic acid dye |
| CN113237856A (en) * | 2021-04-29 | 2021-08-10 | 中国人民解放军军事科学院军事医学研究院 | Aptamer-based detection Tl+Fluorescent probe of |
Non-Patent Citations (7)
| Title |
|---|
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| DAWEI YANG 等: "A Lead (II) Ion Sensor Based on Selective Recognition of G-quadruplex for Ethyl-substitutive Thioflavin T" * |
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