CN114350626A - Luciferase freeze-dried powder dilution reaction liquid - Google Patents
Luciferase freeze-dried powder dilution reaction liquid Download PDFInfo
- Publication number
- CN114350626A CN114350626A CN202111575835.6A CN202111575835A CN114350626A CN 114350626 A CN114350626 A CN 114350626A CN 202111575835 A CN202111575835 A CN 202111575835A CN 114350626 A CN114350626 A CN 114350626A
- Authority
- CN
- China
- Prior art keywords
- luciferase
- freeze
- reaction solution
- dried powder
- dilution reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention provides luciferase freeze-dried powder dilution reaction liquid which comprises the following components: 0.1-3mM fluorescein, 0.1-1mM polyethylene glycol, 0.1-1mM tris (hydroxymethyl) aminomethane, 0.5-2mM 4-hydroxyethyl piperazine propanesulfonic acid, 0.1-0.5mM magnesium chloride, 0.01-0.3mM potassium chloride, 0.05-0.15mM sodium chloride, 0-3mM glycine, 0-20mM glutathione. The luciferase freeze-dried powder dilution reaction solution provided by the invention can effectively embody the activity of luciferase while maintaining the stability of luciferase dry powder.
Description
Technical Field
The invention relates to the technical field of luciferase dry powder, and in particular relates to luciferase freeze-dried powder dilution reaction liquid.
Background
The firefly luciferase catalyzes luminescence to ATP, luciferin and Mg2+In the presence of a yellow-green fluorescence. Most scientists generally consider that the luciferase is catalyzed by D-luciferin as a catalyst, the D-luciferin emits light by oxidation, and the emission intensity of the fluorescence is directly and positively correlated with the concentration of ATP under certain conditions.
Although the principle of luciferase-catalyzed luminescence is basically known to researchers, the instability of luciferase limits its application prospects, and therefore most of the researchers' major research efforts have been focused on stability studies, such as the study of protective agents or the expression of more stable luciferases by site-directed mutagenesis. The luciferase dry powder has good stability, and can be preserved for a long time without losing activity. However, the buffer solution for diluting the dry powder needs a good proportion to better embody the activity of the luciferase. In order to solve the above situation, the application provides a luciferase freeze-dried powder dilution reaction solution.
Disclosure of Invention
The invention aims to provide a luciferase freeze-dried powder dilution reaction solution, which maintains the stability of luciferase dry powder and can effectively embody the activity of luciferase.
The invention adopts the following technical scheme to solve the technical problems:
a luciferase freeze-dried powder dilution reaction solution comprises the following components: comprises the following components: 0.1-3mM fluorescein, 0.1-1mM polyethylene glycol, 0.1-1mM tris (hydroxymethyl) aminomethane, 0.5-2mM 4-hydroxyethyl piperazine propanesulfonic acid, 0.1-0.5mM magnesium chloride, 0.01-0.3mM potassium chloride, 0.05-0.15mM sodium chloride, 0-3mM glycine, 0-20mM glutathione.
Further, the water content in the diluted reaction liquid is not more than 90%.
Further, the water in the diluted reaction solution is sterile water.
Further, the polyethylene glycol is polyethylene glycol 2000 or polyethylene glycol 4000.
The invention has the advantages that:
(1) the luciferase freeze-dried powder dilution reaction solution can well embody the activity of luciferase in a dry powder dilution state.
(2) The luciferase freeze-dried powder dilution reaction solution provided by the invention is simple in formula and can be suitable for large-scale production.
Drawings
FIG. 1 is a summary diagram of the results of diluted reaction solutions in various embodiments of the present invention.
Detailed Description
The invention is further illustrated by the following examples, which are intended to illustrate, but not to limit the invention further.
In the invention, the water content in the diluted reaction solution is not more than 90 percent, and the water is sterile water;
in the following examples, PEG was polyethylene glycol, Tris was Tris (hydroxymethyl) aminomethane, and HEPPS was 4-hydroxyethylpiperazine propanesulfonic acid. In the following embodiments, the polyethylene glycol is polyethylene glycol 2000, the control group distribution ratios are different, and the optimal ratio is measured by an ATP bioluminescence detector.
The invention is further illustrated by the following specific examples:
example 1
The detection method comprises the following steps: dissolving luciferase lyophilized powder 100MG in a centrifuge tube with prepared dilution buffer solution to reach volume of 10ML, and collecting 10ul 1 × 10-9Adding 500ul of dissolved reaction solution into the M ATP standard solution in a reaction tube, placing the reaction tube into a handheld ATP bioluminescence detector for detection, and recording detection data. The above detection steps were repeated 10 times to obtain 10 sets of data. The results are summarized in table 1 below:
table 1: test results obtained in example 1
Example 2
table 2: test results obtained in example 2
Example 3
table 3: test results obtained in example 3
Example 4
table 4: test results obtained in example 4
Example 5
table 5: test results obtained in example 5
The results of the above examples show that, after comprehensive comparison, the luciferase lyophilized powder provided in example 3 is diluted to an optimal ratio.
However, the average of the 10 data in example 1 was 4541.6RLU, the average of the 10 data in example 2 was 6439.7RLU, the average of the 10 data in example 3 was 7582.7RLU, the average of the 10 data in example 4 was 7637.2RLU, and the average of the 10 data in example 5 was 6391.7RLU, in terms of the average ATP content; the average value of the detected ATP content is the highest in the embodiment 4, and the ratio of the diluted reaction solution of the luciferase freeze-dried powder is the most excellent; therefore, the luciferase activity can be well embodied by the luciferase freeze-dried powder dilution reaction liquid formula provided by the application.
Finally, it should be noted that: the above embodiments are only used to illustrate the present invention and do not limit the technical solutions described in the present invention; it will be understood by those skilled in the art that the present invention may be modified and equivalents may be substituted; all such modifications and variations are intended to be included herein within the scope of this disclosure and the present invention and protected by the following claims.
Claims (4)
1. A luciferase freeze-dried powder dilution reaction solution is characterized by comprising the following components: 0.1-3mM fluorescein, 0.1-1mM polyethylene glycol, 0.1-1mM tris (hydroxymethyl) aminomethane, 0.5-2mM 4-hydroxyethyl piperazine propanesulfonic acid, 0.1-0.5mM magnesium chloride, 0.01-0.3mM potassium chloride, 0.05-0.15mM sodium chloride, 0-3mM glycine, 0-20mM glutathione.
2. The luciferase lyophilized powder dilution reaction solution as claimed in claim 1, wherein the water content in the dilution reaction solution is not more than 90%.
3. The luciferase freeze-dried powder dilution reaction solution as claimed in claim 2, wherein water in the dilution reaction solution is sterile water.
4. The luciferase lyophilized powder dilution reaction solution as claimed in claim 1, wherein the polyethylene glycol is polyethylene glycol 2000 or polyethylene glycol 4000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111575835.6A CN114350626A (en) | 2021-12-21 | 2021-12-21 | Luciferase freeze-dried powder dilution reaction liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111575835.6A CN114350626A (en) | 2021-12-21 | 2021-12-21 | Luciferase freeze-dried powder dilution reaction liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114350626A true CN114350626A (en) | 2022-04-15 |
Family
ID=81101190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111575835.6A Pending CN114350626A (en) | 2021-12-21 | 2021-12-21 | Luciferase freeze-dried powder dilution reaction liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114350626A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5283179A (en) * | 1990-09-10 | 1994-02-01 | Promega Corporation | Luciferase assay method |
| WO1994011528A1 (en) * | 1992-11-17 | 1994-05-26 | Celsis Limited | Bioluminescence reagent formulation |
| CN101126718A (en) * | 2006-08-16 | 2008-02-20 | 中国科学院电子学研究所 | Surface cleaning detection reagent for sanitation monitoring |
| CN101218354A (en) * | 2005-05-13 | 2008-07-09 | 珀金埃尔默生命与分析科学有限公司 | Use of ammonium and phosphate ions for improving the detection of luciferase |
| CN103757089A (en) * | 2014-01-10 | 2014-04-30 | 广东省微生物研究所 | Adenosine triphosphate (ATP) bioluminescent reagent for detecting hygienic quality of drinking water and surface sanitation of GMP factory, method and kit |
| CN105018457A (en) * | 2015-07-20 | 2015-11-04 | 宁波美成生物科技有限公司 | Luciferase stabilizer |
-
2021
- 2021-12-21 CN CN202111575835.6A patent/CN114350626A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5283179A (en) * | 1990-09-10 | 1994-02-01 | Promega Corporation | Luciferase assay method |
| WO1994011528A1 (en) * | 1992-11-17 | 1994-05-26 | Celsis Limited | Bioluminescence reagent formulation |
| CN101218354A (en) * | 2005-05-13 | 2008-07-09 | 珀金埃尔默生命与分析科学有限公司 | Use of ammonium and phosphate ions for improving the detection of luciferase |
| CN101126718A (en) * | 2006-08-16 | 2008-02-20 | 中国科学院电子学研究所 | Surface cleaning detection reagent for sanitation monitoring |
| CN103757089A (en) * | 2014-01-10 | 2014-04-30 | 广东省微生物研究所 | Adenosine triphosphate (ATP) bioluminescent reagent for detecting hygienic quality of drinking water and surface sanitation of GMP factory, method and kit |
| CN105018457A (en) * | 2015-07-20 | 2015-11-04 | 宁波美成生物科技有限公司 | Luciferase stabilizer |
Non-Patent Citations (1)
| Title |
|---|
| 薛大权 等: "《聚乙二醇在医药学领域的应用与技术》", 华中科技大学出版社, pages: 43 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Stanley | Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer | |
| JP3409962B2 (en) | Bioluminescent reagent, method for quantifying adenosine phosphate using the reagent, and method for quantifying substances involved in ATP conversion reaction system using the reagent | |
| Naradasu et al. | Isolation and characterization of human gut bacteria capable of extracellular electron transport by electrochemical techniques | |
| CN102382773B (en) | Aspergillus niger strain capable of producing glucose oxidase and application thereof | |
| CN1986785A (en) | Enzyme combining stabilizer | |
| Voulgaris et al. | Inactivating alternative NADH dehydrogenases: enhancing fungal bioprocesses by improving growth and biomass yield? | |
| CN105331589A (en) | Water type NADH oxidase of reproducible coenzyme NAD+ and encoding gene and application thereof | |
| CN108330161A (en) | A kind of significant ATP luciferase assay reagents of thermal stability | |
| Blum et al. | Bioluminescence-and chemiluminescence-based fiberoptic sensors | |
| CN109765220A (en) | Utilize the method for the luminous quantitative detection hydroquinone of cobalt-based metal organogel catalysis luminol-hydrogen peroxide | |
| RU2013112863A (en) | ENZYMATIC REACTIVE INK FOR USE IN TEST STRIPS WITH ADJUSTED CALIBRATION CODE | |
| CN114350626A (en) | Luciferase freeze-dried powder dilution reaction liquid | |
| Wang et al. | Flow injection analysis of glucose based on its inhibition of electrochemiluminescence in a Ru (bpy) 32+–tripropylamine system | |
| CN113758983B (en) | Construction method of glucose biosensor based on glutathione assembly | |
| Takemura et al. | Enhancing acetone production from H2 and CO2 using supplemental electron acceptors in an engineered Moorella thermoacetica | |
| Jathoul et al. | Development of a pH-tolerant thermostable Photinus pyralis luciferase for brighter in vivo imaging | |
| Liu et al. | Tris (2, 2′-bipyridyl) ruthenium (II) electrochemiluminescent detection of coreactants containing aromatic diol group by the interaction between diol and borate anion | |
| CN1800852B (en) | Improved clinical diagnosis reagent for detection by rate method | |
| Gafni | Chiral discrimination in the quenching of an enzyme-bound fluorescent NAD+ analog by an optically active quencher | |
| CN109957603A (en) | A kind of lactate acid detection kit | |
| Moroz et al. | Stabilization of ATP reagents containing firefly L. mingrelica luciferase by polyols | |
| JP2013042728A (en) | Microbial metabolism control device | |
| Wang et al. | A highly selective and light-up red emissive fluorescent probe for imaging of penicillin G amidase in Bacillus cereus | |
| JP2006280365A (en) | Method for detecting adenosine triphosphate and reagent for detecting the same | |
| CN111239111A (en) | g-C3N4VB (vitamin B) detection by nanosheet12In (1) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |