CN114305792A - Ligament regeneration scaffold with layer-by-layer induction performance and preparation method thereof - Google Patents
Ligament regeneration scaffold with layer-by-layer induction performance and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a ligament regeneration scaffold with layer-by-layer induction performance and a preparation method thereof. The ligament regeneration bracket has a multilayer composite structure and is formed by overlapping and compounding a gradient degraded micro-fiber reinforcing layer and an oriented nanofiber inducing layer; the nanofiber induction layer is prepared from a high polymer material and bioactive components through electrostatic spinning. The preparation method comprises the following steps: preparing a micron fiber reinforced layer, preparing a nanofiber inducing layer, and then compounding layer by layer to prepare the ligament regeneration scaffold with the layer-by-layer inducing performance. Based on the regulation and control of the gradient degradation structure, the gradient degradation of the micron fiber reinforced layer is realized while the initial mechanical strength is ensured, and the nanofiber inducing layer is exposed layer by layer to accurately regulate and control the infiltration and remodeling of the internal tissues of the stent, so that the scaffold can be used for ligament/tendon regeneration reconstruction treatment.
Description
Technical Field
The invention relates to a ligament regeneration scaffold with layer-by-layer induction performance and a preparation method thereof, belonging to the technical field of medical instruments.
Background
The anterior cruciate ligament is the important ligament in the knee joint that connects the femur and tibia, starting at the back of the femur, and running obliquely in the joint cavity, ending at the anterior side of the tibia. The anterior cruciate and other ligaments act together to ensure normal movement of the body and maintain the stability of the knee. In recent years, the incidence of athletic injuries has increased year by year as the number of people participating in sports activities has increased, and among all injuries, injury to the anterior cruciate ligament is the most common. However, because of the lack of blood vessels and cells, the injured anterior cruciate ligament is difficult to self-heal, and the anterior cruciate ligament is usually reconstructed clinically by adopting a surgical operation.
The reconstruction of the anterior cruciate ligament mainly comprises three modes of autograft, allograft and artificial ligament. The advantages of autograft include good local vascular reconstruction, low immune response, easy integration with the tissue surrounding the implant, etc. However, autografts do not guarantee complete restoration of the affected area function to the pre-injury level, and various complications and pain may occur in the donor area. Compared with autografting, the allografting has no problem of donor site complication, and can also shorten the operation time without size limitation. However, allograft transplantation presents immunological rejection and may also risk the spread of disease. In view of the many drawbacks of autografting and allograft transplantation, artificial ligaments are increasingly being moved into the field of vision of people. An artificial ligament refers to a ligament substitute made of natural or synthetic polymer materials through some molding processes, and can be used for replacing or assisting in repairing damaged ligaments. Compared with autograft and allograft, the artificial ligament has the advantages of no donor complications, no immune rejection, no risk of disease transmission and the like, and becomes a research hotspot in recent years.
The artificial ligament which is used in clinic is mostly prepared by taking non-degradable material as raw material. While these grafts have sufficient initial tensile strength and may have good recovery for short term use, problems can arise with long term use. The tissue is difficult to grow into the artificial ligament, and even if the time reaches several months, the collagen fiber can only infiltrate the first two layers of the multilayered structure graft; some collagen fibers can penetrate into the artificial ligament, but cannot form an ordered tissue and an ordered arrangement to form an unordered scar tissue, so that the nascent ligament is dysplastic.
Therefore, degradable artificial ligaments have become a research hotspot in recent years, for example, the invention patent with the authorization number of CN104043151B provides a composite artificial ligament and a preparation method thereof, and the artificial ligament with a rod-shaped structure is formed by winding a plurality of tows with different degradation performances into a wire, then weaving the wire into a net-shaped structure, and finally rolling the wire into the artificial ligament. Although the patent utilizes a gradient degradation space to guide tissue ingrowth, collagen arrangement cannot be induced by contact guidance after the tissue ingrowth, and the oriented growth of collagen and tissues after implantation cannot be ensured. The invention patent with the authorization number of CN108653811B provides an artificial ligament and a preparation method thereof, wherein the surface of a semi-finished product of the artificial ligament is spun through an electrostatic spinning technology to form a collagen nanofiber layer, so that the artificial ligament is obtained. The collagen nanofiber layer on the outer surface of the patent can simulate extracellular matrix, so that the biocompatibility of the artificial ligament is increased, but the collagen nanofiber layer is not formed in the ligament stent, and the continuous induction effect cannot be provided after the tissue grows in. In the prior invention patent aiming at the artificial ligament, the problem of tissue infiltration to the inside of the stent is focused on, but the tissue and collagen can not be orderly arranged after the tissue infiltration, or the problem of tissue and collagen orderly arranged on the surface of the stent can be focused on, but the tissue and collagen infiltration can not be ensured, and even if the tissue and collagen can be infiltrated, the tissue and collagen can not be ensured to be orderly arranged in the stent.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the existing artificial ligament can not simultaneously ensure that tissues and collagen infiltrate the inside of the stent and keep ordered arrangement in the stent.
In order to solve the technical problem, the invention provides a ligament regeneration scaffold with layer-by-layer induction performance, which has a multilayer composite structure and is formed by overlapping and compounding a microfiber reinforcing layer with a degradation period gradually increasing layer by layer from outside to inside and a nanofiber induction layer arranged in an oriented manner; the nanofiber inducing layer is prepared by taking the microfiber reinforcing layer as a receiving substrate and carrying out electrostatic spinning on a composite spinning solution, wherein the composite spinning solution contains a high polymer material and bioactive components.
Preferably, the ligament regeneration scaffold has a 3-30-layer composite structure.
Preferably, the yarns with different degradation periods are selected from at least two of silk, PGA, PLGA, PPDO, PCL, PLA and P4 HB; the yarn is monofilament, multifilament, twisted or braided.
Preferably, the polymer material is at least one of P4HB, PHBV, PHA, P (LLA-CL), PPDO, PLGA, PGA, PEG, PGCL, PCL and PLA; the bioactive component is at least one of tilapia collagen, bovine achilles tendon collagen, porcine collagen, gelatin, fibroin, fibrin, elastin, chitosan, alginate and hyaluronic acid.
The invention also provides a preparation method of the ligament regeneration scaffold with layer-by-layer induction performance, which comprises the following steps:
step 1: selecting yarns with different degradation periods, determining a bracket structure, and weaving, knitting or braiding on a machine to prepare a plurality of groups of micron fiber reinforced layers, wherein the degradation periods of the plurality of groups of micron fiber reinforced layers are distributed in a gradient manner;
step 2: cleaning and drying the micron fiber reinforced layer prepared in the step 1;
and step 3: preparing a composite spinning stock solution from a high polymer material and bioactive components, taking the micro fiber reinforced layers obtained by the treatment in the step 2 as a receiving substrate, performing electrostatic spinning, and preparing a nanofiber inducing layer on each group of micro fiber reinforced layers to obtain a plurality of groups of composite layers consisting of the micro fiber reinforced layers and the nanofiber inducing layers;
and 4, step 4: and (3) overlapping and compounding the micro fiber reinforced layer and the nanofiber inducing layer to form a multi-layer composite structure according to the sequence that the degradation period of the micro fiber reinforced layer is gradually increased from outside to inside, and cleaning, drying and sterilizing to obtain the ligament regeneration scaffold with the layer-by-layer inducing performance. Wherein, the overlapping compounding mode can be layer-by-layer superposition.
Preferably, the washing in step 2 is: and cleaning by adopting 70-80 wt% of ethanol water solution, wherein the drying temperature is 35-40 ℃.
Preferably, the specific preparation method of the composite spinning solution in the step 3 is as follows: mixing a high polymer material and a bioactive component according to a mass ratio of 9: 1-1: 9, and uniformly dissolving in a solvent.
Preferably, the solvent is at least one of hexafluoroisopropanol, N-dimethylformamide, N-dimethylacetamide, dimethylsulfoxide, acetone, dichloromethane, chloroform, tetrahydrofuran, 1, 4-dioxane, methane, trifluoroacetic acid, trifluoroethanol, ultrapure water and an aqueous acetic acid solution.
Preferably, the cleaning method in step 4 is as follows: cleaning with ethanol, wherein the sterilization method comprises the following steps: sterilizing with ethylene oxide.
Preferably, when the woven material is woven on the machine in the step 1, a micron fiber reinforced layer with the longest degradation period is prepared first, the composite layer composed of a single group of micron fiber reinforced layers and a nanofiber inducing layer is prepared in the step 3, and the step 4 is to weave the next group of micron fiber reinforced layers on the composite layer prepared in the step 3, that is, the operations in the steps 1 to 3 are repeated until the ligament regeneration scaffold is prepared.
Wherein the diameters of the selected yarns with different degradation periods are 0.05-0.5 mm; the aperture size of the micron fiber reinforced layer is 10-30 mu m, and the thickness is 0.1-1.5 mm; the pore size of the nanofiber inducing layer is 0.5-15 mu m, and the thickness of the nanofiber inducing layer is 0.005-0.1 mm; the prepared ligament regeneration scaffold is 5-70 mm in length, 1-10 mm in thickness and 1-15 mm in width.
The ligament regeneration scaffold with the layer-by-layer induction performance is characterized in that an oriented nanofiber induction layer is prepared by utilizing an electrostatic spinning technology, and cells and collagen are induced to be arranged in order; the multi-dimensional multi-spinning forming weaving is utilized to prepare the micron fiber reinforced layer with gradient degradation, the mechanical strength at the initial stage is ensured, the gradient degradation is realized, and the nanofiber inducing layer is exposed layer by layer. The gradient degradation of the micron fiber reinforced layer gives up space for the growth of tissues and collagen, and induces the infiltration of the tissues and the collagen; the orientation arrangement of the nanofiber inducing layer provides a certain topographic clue for the growth of cells and tissues, and induces the oriented growth of the tissues and collagen in the scaffold; the biological active components contained in the nanofiber inducing layer provide certain biochemical clues for the growth of cells and tissues, so that the growth activity of the cells and the tissues is improved, and the effect of synergistically inducing the regeneration of the tissues is further realized.
The ligament regeneration scaffold with the layer-by-layer induction performance and the common PET scaffold and fibroblast in-vitro culture show that more cell proliferation can be observed on the ligament regeneration scaffold with the layer-by-layer induction performance, the cell spreadability is good, the cells are in a slender spindle-shaped form, the cell activity is improved by 30-70%, the cell penetration depth in the scaffold is improved by 20-50%, meanwhile, the ordered arrangement of tissues and collagen fibers can be realized, and the remodeling of the tissues and the collagen fibers is promoted.
Compared with the prior art, the invention has the beneficial effects that:
1. the ligament regeneration scaffold with the layer-by-layer induction performance, which is prepared by the invention, realizes the initial high mechanical performance by a multilayer structure formed by overlapping and compounding a gradient degraded micro fiber reinforcing layer and an oriented nanofiber induction layer;
2. according to the ligament regeneration scaffold with the layer-by-layer induction performance, the oriented arrangement of the nanofibers in the nanofiber induction layer can be realized by adjusting the spinning parameters, so that contact guidance is provided for cells, and the growth of the cells along the fiber orientation direction and the oriented arrangement of collagen fibers are promoted;
3. the ligament regeneration scaffold with the layer-by-layer induction performance can form a gradient degradable microfiber reinforcing layer by adjusting the types and the proportion of yarns with different degradation periods, degrade layer by layer after being implanted, expose the surface of the oriented nanofiber induction layer by layer, continuously induce the cells to grow in, and orient and arrange the cells in the scaffold.
Drawings
FIG. 1 is a schematic structural diagram of a ligament regeneration scaffold with layer-by-layer induction capability according to the present invention; I. II respectively represents an oriented nanofiber inducing layer and a gradient degraded microfiber reinforcing layer, wherein 1-a and 1-b represent yarns with different degradation periods which form the microfiber reinforcing layer;
FIG. 2 is a schematic structural diagram of a ligament regeneration scaffold with layer-by-layer induction performance prepared in example 1 and a microfiber reinforcing layer prepared by weaving in step 2 of example 1; 2-a represents a yarn with a long degradation time, and 2-b represents a yarn with a short degradation time;
FIG. 3 is a schematic structural diagram of a ligament regeneration scaffold with layer-by-layer induction performance prepared in example 2 and a microfiber reinforcement layer prepared by knitting in step 2 of example 2; 3-a represents a yarn with a long degradation time, and 3-b represents a yarn with a short degradation time;
FIG. 4 is a schematic structural diagram of a ligament regeneration scaffold with layer-by-layer induction performance prepared in example 3 and a microfiber reinforcement layer prepared by weaving in step 2 of example 3; 4-a represents a yarn with a long degradation time, and 4-b represents a yarn with a short degradation time.
Detailed Description
In order to make the invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.
Example 1
A preparation method of a ligament regeneration scaffold with layer-by-layer induction performance comprises the following steps:
step 1: the silk braided wire (2-a) and the PGA (2-b) monofilament are selected as yarns with different degradation periods.
Step 2: and preparing a micron fiber reinforced layer. Determining a bracket structure, selecting plain weave, using silk as warp, and using silk and PGA (poly-propylene glycol) as weft at intervals by a weaving method (figure 2), and weaving on a machine to obtain the weft with the ratio of silk: PGA 1: 3 and silk: PGA 1: 1 two micron fiber reinforced layers (II-1, II-2).
And step 3: and (5) cleaning and drying. And (3) cleaning the micron fiber reinforced layer prepared in the step (2) by using 75% ethanol, and drying at 37 ℃ for later use.
And 4, step 4: preparing a nanofiber inducing layer (I). Mixing bovine achilles tendon collagen and PCL according to a mass ratio of 15: 85, and then uniformly dissolving the mixture in hexafluoroisopropanol to obtain a composite spinning solution; and (4) taking the two micron fiber reinforced layers obtained in the step (3) as receiving substrates, and preparing the nanofiber inducing layer from the composite spinning solution by adopting a solution electrostatic spinning process. Wherein the spinning voltage is 15KV, the receiving distance is 16cm, the roller rotating speed is 1600rpm, the injection speed is 0.8ml/h, and the spinning time is 1 h.
And 5: will carry two kinds of micron fiber enhancement layers successive layer overlap of nanofiber induced layer, form upper strata and lower floor and be silk: PGA 1: 3, the middle layer is silk: PGA 1: 1, sewing and reinforcing the bracket by silk braided wires, cleaning the bracket by using ethanol, drying the cleaned bracket, and sterilizing the cleaned bracket by using ethylene oxide to obtain the bracket (shown in figure 2).
The prepared ligament regeneration scaffold and the common PET scaffold and fibroblast in-vitro culture show that more cell proliferation can be observed on the ligament regeneration scaffold with layer-by-layer induction performance, the cell spreadability is good, the cells are in a slender spindle-shaped form, the cell activity is improved by 30%, the cell penetration depth in the scaffold is improved by 40%, and meanwhile, the ordered arrangement of tissues and collagen fibers can be realized, and the remodeling of the tissues and the collagen fibers is promoted.
Example 2
A preparation method of a ligament regeneration scaffold with layer-by-layer induction performance comprises the following steps:
step 1: PCL (3-a) monofilament and PLGA (3-b) monofilament are selected as yarns with different degradation periods.
Step 2: and preparing a micron fiber reinforced layer. Determining a support structure, selecting a weft plain stitch tissue and PCL and PLGA interval configuration by adopting a knitting method (figure 3), and carrying out weaving on a machine to obtain a yarn with the ratio of PCL: PLGA ═ 1: 3. PCL: PLGA ═ 1: 2. PCL: PLGA ═ 1: 1 (II-1, II-2, II-3).
And step 3: and (5) cleaning and drying. And (3) cleaning the micron fiber reinforced layer prepared in the step (2) by using 75% ethanol, and drying at 37 ℃ for later use.
And 4, step 4: preparing a nanofiber inducing layer (I). Mixing gelatin and PGCL in a mass ratio of 30: 70, uniformly dissolving in trifluoroethanol to obtain a composite spinning solution; and 3, taking the three micron fiber reinforced layers obtained in the step 3 as receiving substrates, and preparing the nanofiber inducing layer from the composite spinning solution by adopting a solution electrostatic spinning process. Wherein the spinning voltage is 16KV, the receiving distance is 14cm, the roller rotation speed is 1800rpm, the injection speed is 0.6ml/h, and the spinning time is 2 h.
And 5: overlapping three kinds of micro fiber reinforcement bodies loaded with the nanofiber inducing layer by layer to form two layers of PCL on the outermost layer: PLGA ═ 1: 3. the secondary outer layer is PCL: PLGA ═ 1: 2. the innermost layer is PCL: PLGA ═ 1: 1, sewing and reinforcing by PCL thread, cleaning by ethanol, drying, and sterilizing by ethylene oxide to obtain the stent (figure 3).
The prepared ligament regeneration scaffold and the common PET scaffold and fibroblast in-vitro culture show that more cell proliferation can be observed on the ligament regeneration scaffold with layer-by-layer induction performance, the cell spreadability is good, the cells are in a slender spindle-shaped form, the cell activity is improved by 50%, the cell penetration depth in the scaffold is improved by 30%, meanwhile, the ordered arrangement of tissues and collagen fibers can be realized, and the remodeling of the tissues and the collagen fibers is promoted.
Example 3
A preparation method of a ligament regeneration scaffold with layer-by-layer induction performance comprises the following steps:
step 1: PPDO (4-a) monofilament and PGA (4-b) monofilament were selected as yarns having different degradation periods.
Step 2: and preparing a micron fiber reinforced layer. Determining a support structure, adopting a weaving method (figure 4), carrying out 12-spindle weaving, PPDO and PGA are arranged at intervals, carrying out machine weaving, and firstly obtaining PPDO: PGA 1: 1 micron fiber reinforced layer (II-3).
And step 3: and (5) cleaning and drying. And (3) cleaning the micron fiber reinforced layer prepared in the step (2) by using 75% ethanol, and drying at 37 ℃ for later use.
And 4, step 4: preparing a nanofiber inducing layer (I). Mixing hyaluronic acid and PPDO according to a mass ratio of 20: 80, and uniformly dissolving the mixture in hexafluoroisopropanol to obtain a composite spinning solution; and 3, taking the micron fiber reinforced layer obtained in the step 3 as a receiving substrate, and preparing the nano fiber inducing layer from the composite spinning solution by adopting a solution electrostatic spinning process. Wherein the spinning voltage is 14KV, the receiving distance is 16cm, the roller rotating speed is 1700rpm, the injection speed is 0.8ml/h, and the spinning time is 1.5 h.
And 5: and (3) taking the micron fiber reinforced layer loaded with the nanofiber inducing layer obtained in the step (4) as core yarn, and weaving 12 spindles in the proportion of PPDO: PGA 1: 2, obtaining the micron fiber reinforced layer (II-2).
Step 6: and (5) cleaning and drying. And (4) cleaning the micron fiber reinforced layer prepared in the step (5) by using 75% ethanol, and drying at 37 ℃ for later use.
And 7: preparing a nanofiber inducing layer (I). Mixing hyaluronic acid and PPDO according to a mass ratio of 20: 80, and uniformly dissolving the mixture in hexafluoroisopropanol to obtain a composite spinning solution; and (4) taking the micron fiber reinforced layer obtained in the step (6) as a receiving substrate, and preparing the nano fiber inducing layer from the composite spinning solution by adopting a solution electrostatic spinning process. The spinning parameters were as described in step 4.
And 8: and (3) taking the micron fiber reinforced layer loaded with the nanofiber inducing layer obtained in the step (7) as core yarn, and weaving 12 spindles in the proportion of PPDO: PGA 1: 3, obtaining the micron fiber reinforced layer (II-1).
And step 9: and (5) cleaning and drying. And (4) cleaning the micron fiber reinforced layer prepared in the step (8) by using 75% ethanol, and drying at 37 ℃ for later use.
Step 10: preparing a nanofiber inducing layer (I). Mixing hyaluronic acid and PPDO according to a mass ratio of 20: 80, and uniformly dissolving the mixture in hexafluoroisopropanol to obtain a composite spinning solution; and (4) taking the micro-fiber reinforced layer obtained in the step (9) as a receiving substrate, and preparing the outermost nano-fiber inducing layer from the composite spinning solution by adopting a solution electrostatic spinning process, wherein the spinning parameters are as described in the step (4). Washing with ethanol, oven drying, and sterilizing with ethylene oxide to obtain the stent (fig. 4).
The prepared regenerated ligament stent and the common PET stent and fibroblast in-vitro culture show that more cell proliferation can be observed on the ligament regeneration stent with layer-by-layer induction performance, the cell spreadability is good, the cells are in a slender spindle-shaped form, the cell activity is improved by 40%, the cell penetration depth in the stent is improved by 40%, meanwhile, the ordered arrangement of tissues and collagen fibers can be realized, and the remodeling of the tissues and the collagen fibers is promoted.
The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way and substantially, it should be noted that those skilled in the art may make several modifications and additions without departing from the scope of the present invention, which should also be construed as a protection scope of the present invention.
Claims (10)
1. A ligament regeneration scaffold with layer-by-layer induction performance is characterized in that the ligament regeneration scaffold has a multilayer composite structure and is formed by overlapping and compounding a micron fiber reinforcement layer with a degradation period increasing layer by layer from outside to inside and a nanofiber induction layer arranged in an oriented manner; the nanofiber inducing layer is prepared by taking the microfiber reinforcing layer as a receiving substrate and carrying out electrostatic spinning on a composite spinning solution, wherein the composite spinning solution contains a high polymer material and bioactive components.
2. The ligament regeneration scaffold with layer-by-layer induction performance according to claim 1, wherein the ligament regeneration scaffold has a composite structure of 3-30 layers.
3. The ligament regeneration scaffold with layer-by-layer induction performance according to claim 1, wherein the yarns with different degradation periods are selected from at least two of silk, PGA, PLGA, PPDO, PCL, PLA and P4 HB; the yarn is monofilament, multifilament, twisted or braided.
4. The ligament regeneration scaffold with layer-by-layer induction performance according to claim 1, wherein the polymer material is at least one of P4HB, PHBV, PHA, P (LLA-CL), PPDO, PLGA, PGA, PEG, PGCL, PCL and PLA; the bioactive component is at least one of tilapia collagen, bovine achilles tendon collagen, porcine collagen, gelatin, fibroin, fibrin, elastin, chitosan, alginate and hyaluronic acid.
5. The method for preparing a ligament regeneration scaffold with layer-by-layer induction performance according to any one of claims 1 to 4, comprising the following steps:
step 1: selecting yarns with different degradation periods, determining a bracket structure, and weaving, knitting or braiding on a machine to prepare a plurality of groups of micron fiber reinforced layers, wherein the degradation periods of the micron fiber reinforced layers are distributed in a gradient manner;
step 2: cleaning and drying the micron fiber reinforced layer prepared in the step 1;
and step 3: preparing a composite spinning stock solution from a high polymer material and bioactive components, taking the micro fiber reinforced layers obtained by the treatment in the step 2 as a receiving substrate, performing electrostatic spinning, and preparing a nanofiber inducing layer on each group of micro fiber reinforced layers to obtain a plurality of groups of composite layers consisting of the micro fiber reinforced layers and the nanofiber inducing layers;
and 4, step 4: and (3) overlapping and compounding the micro fiber reinforced layer and the nanofiber inducing layer to form a multi-layer composite structure according to the sequence that the degradation period of the micro fiber reinforced layer is gradually increased from outside to inside, and cleaning, drying and sterilizing to obtain the ligament regeneration scaffold with the layer-by-layer inducing performance.
6. The method for preparing a ligament regeneration scaffold with layer-by-layer induction performance according to claim 5, wherein the cleaning in the step 2 is as follows: and cleaning by adopting 70-80 wt% of ethanol water solution, wherein the drying temperature is 35-40 ℃.
7. The method for preparing a ligament regeneration scaffold with layer-by-layer induction performance according to claim 5, wherein the specific preparation method of the composite spinning solution in the step 3 is as follows: mixing a high polymer material and a bioactive component according to a mass ratio of 9: 1-1: 9, and uniformly dissolving in a solvent.
8. The method for preparing a ligament regeneration scaffold having layer-by-layer induction properties according to claim 7, wherein the solvent is at least one of hexafluoroisopropanol, N-dimethylformamide, N-dimethylacetamide, dimethylsulfoxide, acetone, dichloromethane, chloroform, tetrahydrofuran, 1, 4-dioxane, methane, trifluoroacetic acid, trifluoroethanol, ultrapure water and an aqueous solution of acetic acid.
9. The method for preparing a ligament regeneration scaffold with layer-by-layer induction performance according to claim 5, wherein the cleaning method in the step 4 is as follows: cleaning with ethanol, wherein the sterilization method comprises the following steps: sterilizing with ethylene oxide.
10. The method for preparing a ligament regeneration scaffold having layer-by-layer induction performance according to claim 5, wherein when the step 1 is woven on a machine by using weaving, a microfiber reinforcing layer having the longest degradation period is prepared, the step 3 is to prepare a composite layer consisting of a single group of the microfiber reinforcing layer and the nanofiber induction layer, and the step 4 is to weave the next group of the microfiber reinforcing layer on the composite layer prepared in the step 3, that is, the operations of the steps 1 to 3 are repeated until the preparation of the ligament regeneration scaffold is completed.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115068687A (en) * | 2022-07-08 | 2022-09-20 | 重庆科技学院 | Gradient nano/microfiber scaffold and preparation method and application thereof |
| CN115845136A (en) * | 2022-12-15 | 2023-03-28 | 南京市第一医院 | Near-field direct-writing electrostatic spinning 3D bionic tendon bone repair support and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115068687A (en) * | 2022-07-08 | 2022-09-20 | 重庆科技学院 | Gradient nano/microfiber scaffold and preparation method and application thereof |
| CN115068687B (en) * | 2022-07-08 | 2023-12-12 | 重庆科技学院 | Gradient nano/micro fiber support and preparation method and application thereof |
| CN115845136A (en) * | 2022-12-15 | 2023-03-28 | 南京市第一医院 | Near-field direct-writing electrostatic spinning 3D bionic tendon bone repair support and preparation method thereof |
| CN115845136B (en) * | 2022-12-15 | 2024-01-26 | 南京市第一医院 | Near-field direct-writing electrostatic spinning 3D bionic tendon-bone repair stent and preparation method thereof |
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