CN114304053A - Construction method of rabbit aneurysm model - Google Patents
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Abstract
The invention discloses a construction method of a rabbit aneurysm model, which comprises the following steps: s1: n different rabbit aneurysm model building methods are selected to build N groups of rabbit aneurysm models, wherein N is an integer greater than or equal to 2, and each model building method corresponds to one group of rabbit aneurysm models; s2: measuring the N groups of rabbit aneurysm models obtained in the step S1 by adopting DSA equipment to obtain the short diameter of the rabbit aneurysm bodies of each group; s3: and D, performing comparative analysis on the N groups of rabbit aneurysm body short diameter data obtained in the step S2, and selecting a construction method corresponding to the rabbit aneurysm model with the largest tumor body short diameter to obtain the rabbit aneurysm. The method for constructing the aneurysm model has the advantages of high success rate, convenience in research and the like.
Description
Technical Field
The invention relates to the field of animal tumor models, in particular to a construction method of a rabbit aneurysm model.
Background
Aneurysm is a manifestation of localized or diffuse expansion or bulging of an artery wall due to lesion or injury of the artery wall, mainly manifested by a swelling or pulsating mass, and serious patients may suffer from arterial embolism, organ ischemia or necrosis, etc., thereby causing serious damage to human bodies. Since human aneurysms are found in a substantially advanced stage, it is difficult to deeply study the mechanism of aneurysm formation and treatment. Therefore, establishing a feasible aneurysm model with good repeatability is a precondition for research on aneurysm pathology, treatment method and the like. For this reason, a series of studies have been conducted, but how to obtain an effective and easy-to-study aneurysm model remains to be studied.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a construction method for effectively obtaining a rabbit aneurysm model with high success rate and convenient research.
In order to achieve the purpose, the invention adopts the following technical scheme:
a construction method of a rabbit aneurysm model comprises the following steps:
s1: n different rabbit aneurysm model building methods are selected to build N groups of rabbit aneurysm models, wherein N is an integer greater than or equal to 2, and each model building method corresponds to one group of rabbit aneurysm models;
s2: measuring the N groups of rabbit aneurysm models obtained in the step S1 by adopting DSA equipment to obtain short diameter data of rabbit aneurysm bodies of each group (as the whole aneurysm body is in an ellipsoid shape, the long diameter and the short diameter exist, and the short diameter data are obtained in the invention);
s3: and D, performing comparative analysis on the N groups of rabbit aneurysm body short diameter data obtained in the step S2, and selecting a construction method corresponding to the rabbit aneurysm model with the largest tumor body short diameter to obtain the rabbit aneurysm.
In the present invention, a further preferred embodiment is that the rabbit aneurysm model establishing method includes the following steps: constructing a closed sac at the root of the right common carotid artery of the rabbit, injecting an aneurysm inducer into the sac, cleaning the aneurysm inducer in the sac of the rabbit arterial artery after 20-30min, and then recovering the smoothness of the rabbit artery to obtain the rabbit aneurysm model.
In a more preferred embodiment of the present invention, the aneurysm-inducing agent is one or a combination of two or more of elastase, papain, calcium chloride, and collagenase.
In the present invention, a further preferred scheme is that the specific steps of constructing the closed capsule cavity are as follows: clamping or tightening two ends of one section of the middle part of the rabbit artery to form a rabbit artery closed cavity, pumping blood in the rabbit artery closed cavity, and cleaning the blood in the rabbit artery closed cavity by using normal saline containing heparin sodium to obtain the closed sac cavity.
In the present invention, it is further preferable that the rabbit aneurysm model is a rabbit carotid aneurysm model.
In the present invention, it is further preferable that the number of effective rabbit aneurysm models in each group in step S1 is at least 2, and the short diameter data of rabbit aneurysm body of each group in step S2 is an average of the short diameter data of effective rabbit aneurysm models of each group; the effective rabbit aneurysm model is a model of aneurysm.
In the present invention, a further preferred scheme is that, in step S1, 3 different rabbit aneurysm model establishment methods are selected to construct 3 groups of rabbit aneurysm models, wherein:
the method for establishing the first group of rabbit aneurysm models specifically comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of the rabbit, cleaning the closed cavity by using normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 70U into the rabbit artery closed cavity, keeping the peripheral tissues of the rabbit carotid artery moist by using the normal saline, further ligating the far end of the carotid artery after 20-30min, recovering the artery to be smooth, and feeding for more than 28 days (the invention contains the original number), thus obtaining the rabbit arterial closed cavity;
the method for establishing the second group of rabbit aneurysm models comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the artery unobstructed, and feeding for more than 28 days to obtain the rabbit arterial closed cavity;
the method for establishing the third group of rabbit aneurysm models comprises the following specific steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood remains, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the smoothness of the artery, feeding for more than 28 days, and feeding the animal with double antibodies every day during feeding to obtain the rabbit.
In the present invention, it is further preferable that the rabbit aneurysm model building method in step S1 is implemented by using one or a combination of two or more of an elastase induction method, a papain induction method, a calcium chloride induction method, an elastase + collagenase induction method, and an adenovirus induction method.
In the present invention, it is further preferable that the time for measuring the tumor body minor diameter of the rabbit aneurysm model using the DSA apparatus in step S2 is 28 days or more after the rabbit aneurysm model is established in step S1.
Compared with the prior art, the invention has the following beneficial effects:
1) different rabbit aneurysm models are established by selecting different aneurysm model establishing methods, then DSA image measurement is carried out, comparison is carried out, and the establishing method corresponding to the rabbit aneurysm model with the largest tumor body minor diameter is screened out, so that the rabbit aneurysm model with high success rate, good aneurysm forming effect and convenience in research can be obtained, and the subsequent research on the aneurysm can be better guided;
2) through analysis, a model construction method for optimizing the concentration of elastase is determined, the intima of the blood vessel is fully destroyed, and the success rate of forming aneurysm is improved;
3) through analysis, a model construction method of the continuous feeding double antibody is screened out, thrombosis at the distal end of the aneurysm is reduced, the possibility of self-occlusion of the aneurysm is reduced, and therefore the aneurysm model is obtained.
Drawings
FIG. 1 is an angiogram of a rabbit aneurysm model obtained by DSA device testing according to the first rabbit aneurysm model building method in example 1;
FIG. 2 is a angiogram obtained by DSA equipment testing of a rabbit aneurysm model obtained by the first rabbit aneurysm model establishing method in example 1;
FIG. 3 is an angiogram obtained by DSA equipment testing of a rabbit aneurysm model obtained by the establishing method of the second group of rabbit aneurysm models in embodiment 1;
FIG. 4 is a angiogram obtained by DSA equipment testing of a rabbit aneurysm model obtained by the establishing method of the second group of rabbit aneurysm models in embodiment 1;
FIG. 5 is a angiogram obtained by DSA equipment testing of a rabbit aneurysm model obtained by the method for establishing a third group of rabbit aneurysm models in example 1;
fig. 6 is an angiogram obtained by DSA device test of a rabbit aneurysm model obtained by the method for establishing a rabbit aneurysm model of the third group in example 1.
Detailed Description
The present invention is further described below with reference to the following detailed description and the accompanying drawings, and it should be noted that, in the present invention, various embodiments or technical features described below may be arbitrarily combined to form a new embodiment without conflict. Except as specifically noted, the materials and equipment used in this example are commercially available. The specific embodiments are merely illustrative and are not to be construed as limiting the scope of the invention.
A construction method of a rabbit aneurysm model comprises the following steps:
s1: n different rabbit aneurysm model building methods are selected to build N groups of rabbit aneurysm models, wherein N is an integer greater than or equal to 2, and each model building method corresponds to one group of rabbit aneurysm models;
s2: measuring the N groups of rabbit aneurysm models obtained in the step S1 by adopting DSA equipment to obtain short diameter data of rabbit aneurysm bodies of each group (as the whole aneurysm body is in an ellipsoid shape, the long diameter and the short diameter exist, and the short diameter data are obtained in the invention);
s3: and D, performing comparative analysis on the N groups of rabbit aneurysm body short diameter data obtained in the step S2, and selecting a construction method corresponding to the rabbit aneurysm model with the largest tumor body short diameter to obtain the rabbit aneurysm.
Different rabbit aneurysm models are established by selecting different aneurysm model establishing methods, then DSA image measurement is carried out, comparison is carried out, and the establishing method corresponding to the rabbit aneurysm model with the largest tumor body minor diameter is screened out, so that the rabbit aneurysm model with high success rate, good aneurysm forming effect and convenience in research can be obtained, and follow-up research on aneurysms can be better guided.
The method for establishing the rabbit aneurysm model can be obtained by the following method, and specifically comprises the following steps: constructing a closed sac at the root of the right common carotid artery of the rabbit, injecting an aneurysm inducer into the sac, cleaning the aneurysm inducer in the sac of the rabbit arterial artery after 20-30min, recovering the smoothness of the rabbit artery, and obtaining the rabbit aneurysm model after at least 28 days.
The aneurysm inducer may be one or a combination of two or more of elastase, papain, calcium chloride, and collagenase; by means of the aneurysm-inducing agent, damage can be caused to the arterial wall, thereby inducing aneurysm.
For the method for constructing the closed sac cavity, a section of artery is generally clamped or fastened at two ends to form a sac cavity; the specific steps for constructing the rabbit artery sac cavity can be exemplified by: clamping or tightening two ends of one section of the middle part of the rabbit artery to form a rabbit artery closed cavity, pumping blood in the rabbit artery closed cavity, and cleaning the blood in the rabbit artery closed cavity by using normal saline containing heparin sodium to obtain the closed sac cavity.
The types of rabbit aneurysm models can be selected according to positions or research requirements, such as celiac arteries, lower limbs and the like; the rabbit common carotid artery is selected, so that the operation and the research are more convenient.
In order to reduce errors or accidental factors of a single experiment, the number of effective rabbit aneurysm models in each group in the step S1 is at least 2, and the short diameter data of the rabbit aneurysm body of each group in the step 2 is the average of the short diameter data of the effective rabbit aneurysm models of each group; the effective rabbit aneurysm model is a model in which an aneurysm is formed; the reliability of the related data can be further improved by averaging a plurality of data, and the model construction method can be better determined.
As for the method for establishing the rabbit aneurysm model, various existing rabbit aneurysm model establishing methods can be selected, and for example, 3 different rabbit aneurysm model establishing methods are selected in step S1 to establish 3 groups of rabbit aneurysm models, where:
the method for establishing the first group of rabbit aneurysm models specifically comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of the rabbit, cleaning the closed cavity by using normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 70U into the rabbit artery closed cavity, keeping the peripheral tissues of the rabbit carotid artery moist by using the normal saline, further ligating the far end of the carotid artery after 20-30min, recovering the artery to be smooth, and feeding for more than 28 days (the invention contains the original number), thus obtaining the rabbit arterial closed cavity;
the method for establishing the second group of rabbit aneurysm models comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the artery unobstructed, and feeding for more than 28 days to obtain the rabbit arterial closed cavity;
the method for establishing the third group of rabbit aneurysm models comprises the following specific steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood remains, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the smoothness of the artery, feeding for more than 28 days, and feeding the animal with double antibodies every day during feeding to obtain the rabbit.
For the constructed rabbit aneurysm model, after DSA tumor body short diameter is measured, relevant tumor body short diameter data can be stored, then a relevant database is established, different rabbit aneurysm models can be continuously added subsequently, and more model data corresponding to different construction methods can be obtained; furthermore, the training can be carried out through a convolutional neural network, and a better method can be obtained through model data of multiple samples.
As for the method for establishing the rabbit aneurysm model in step S1, one or a combination of two or more of an elastase induction method, a papain induction method, a calcium chloride induction method, an elastase + collagenase induction method, and an adenovirus induction method may be selected.
For better measurement of DSA data of the rabbit tumor model, the time for measuring the tumor body short diameter of the rabbit aneurysm model by using a DSA device in the step S2 is more than 28 days after the rabbit aneurysm model is established in the step S1.
Example 1
A construction method of a rabbit aneurysm model is characterized by comprising the following steps:
s1: n different rabbit aneurysm model building methods are selected to build N groups of rabbit aneurysm models, wherein N is an integer greater than or equal to 2, and each model building method corresponds to one group of rabbit aneurysm models;
s2: measuring the N groups of rabbit aneurysm models obtained in the step S1 by adopting DSA equipment to obtain short diameter data of rabbit aneurysm bodies of all groups;
s3: performing comparative analysis on the N groups of rabbit aneurysm body short diameter data obtained in the step S2, and selecting a construction method corresponding to the rabbit aneurysm model with the largest tumor body short diameter to obtain the rabbit aneurysm model;
in the step S1, 3 different rabbit aneurysm model building methods are selected to build 3 groups of rabbit aneurysm models, wherein:
the method for establishing the first group of rabbit aneurysm models specifically comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of the rabbit, cleaning the closed cavity by using normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 70U into the rabbit artery closed cavity, keeping the peripheral tissues of the rabbit carotid artery moist by using normal saline, further ligating the far end of the carotid artery after 20-30min, recovering the artery to be smooth, and feeding for 28 days to obtain the rabbit arterial closed cavity;
the method for establishing the second group of rabbit aneurysm models comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the artery unobstructed, and feeding for 28 days to obtain the rabbit arterial closed cavity;
the method for establishing the third group of rabbit aneurysm models comprises the following specific steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood remains, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the smoothness of the artery, feeding for 28 days, and feeding animals with double antibodies every day during feeding to obtain the rabbit arterial occlusive cavity;
then, DSA image measurement is carried out on rabbit aneurysm models (carotid arteries) of different groups, and specific test results are shown in figures 1-6; through analysis and comparison, the data of the third group of models is more optimal, and the corresponding model building method of the group is determined to be a rabbit aneurysm model building method; subsequent experiments show that the method has high tumor formation rate and good reproducibility, and is convenient for researching pathological mechanism, treatment and the like of the aneurysm.
Finally, it should be noted that: the above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention should not be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (9)
1. A construction method of a rabbit aneurysm model is characterized by comprising the following steps:
s1: n different rabbit aneurysm model building methods are selected to build N groups of rabbit aneurysm models, wherein N is an integer greater than or equal to 2, and each model building method corresponds to one group of rabbit aneurysm models;
s2: measuring the N groups of rabbit aneurysm models obtained in the step S1 by adopting DSA equipment to obtain short diameter data of rabbit aneurysm bodies of all groups;
s3: and D, performing comparative analysis on the N groups of rabbit aneurysm body short diameter data obtained in the step S2, and selecting a construction method corresponding to the rabbit aneurysm model with the largest tumor body short diameter to obtain the rabbit aneurysm.
2. The construction method according to claim 1, wherein the rabbit aneurysm model building method comprises the following steps: constructing a closed sac at the root of the right common carotid artery of the rabbit, injecting an aneurysm inducer into the sac, cleaning the aneurysm inducer in the sac of the rabbit arterial artery after 20-30min, and then recovering the smoothness of the rabbit artery to obtain the rabbit aneurysm model.
3. The method according to claim 2, wherein the aneurysm induction agent is one or a combination of two or more of elastase, papain, calcium chloride, and collagenase.
4. The construction method according to claim 2, wherein the specific steps of constructing the closed capsule cavity are as follows: clamping or tightening two ends of one section of the middle part of the rabbit artery to form a rabbit artery closed cavity, pumping blood in the rabbit artery closed cavity, and cleaning the blood in the rabbit artery closed cavity by using normal saline to obtain the closed sac cavity.
5. The method of constructing according to claim 1, wherein the rabbit aneurysm model is a rabbit carotid aneurysm model.
6. The method according to claim 1, wherein the number of effective rabbit aneurysm models in each group in step S1 is at least 2, and the short diameter data of rabbit aneurysm body of each group in step S2 is an average of the short diameter data of the effective rabbit aneurysm models of each group; the effective rabbit aneurysm model is a model of aneurysm.
7. The method according to claim 1, wherein 3 different rabbit aneurysm model building methods are selected in step S1 to build 3 rabbit aneurysm models, wherein:
the method for establishing the first group of rabbit aneurysm models specifically comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of the rabbit, cleaning the closed cavity by using normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 70U into the rabbit artery closed cavity, keeping the peripheral tissues of the rabbit carotid artery moist by using normal saline, further ligating the far end of the carotid artery after 20-30min, recovering the artery to be smooth, and feeding for more than 28 days to obtain the rabbit arterial closed cavity;
the method for establishing the second group of rabbit aneurysm models comprises the following steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood residue exists, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the artery unobstructed, and feeding for more than 28 days to obtain the rabbit arterial closed cavity;
the method for establishing the third group of rabbit aneurysm models comprises the following specific steps: constructing a rabbit artery closed cavity of 1.9-2.1cm at the root of the right carotid artery of a rabbit, cleaning the closed cavity by normal saline containing heparin sodium until no blood remains, injecting 0.1ml of elastase solution containing 200U into the rabbit artery closed cavity, keeping the closed cavity in an inflated state all the time, keeping the peripheral tissues of the rabbit carotid artery moist by normal saline, further ligating the distal end of the carotid artery after 20-30min, recovering the smoothness of the artery, feeding for more than 28 days, and feeding the animal with double antibody every day during feeding to obtain the rabbit.
8. The constructing method according to claim 1, wherein the rabbit aneurysm model establishing method in step S1 is performed by using one or a combination of two or more of elastase induction, papain induction, calcium chloride induction, elastase + collagenase induction, and adenovirus induction.
9. The constructing method according to claim 1, wherein the time for measuring the tumor body minor diameter of the rabbit aneurysm model using a DSA apparatus in step S2 is 28 days or more after the rabbit aneurysm model is established in step S1.
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