CN114303664B - Crape myrtle hardwood cutting propagation method - Google Patents
Crape myrtle hardwood cutting propagation method Download PDFInfo
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Abstract
The invention discloses a crape myrtle hardwood cutting propagation method. The cutting propagation method comprises the following steps: preparing cutting slips, removing cutting slip inhibitors, preparing a matrix, cutting, supplementing an accelerant, hardening off seedlings and the like. And removing the cuttings inhibitor by adopting a method combining solution soaking and sand storage. Adding resin with rooting agent and chlorogenic acid adsorbed in the matrix. And (3) adding a step of slowly releasing zeatin for promoting the growth of lateral buds in the cutting process. The method is suitable for the lagerstroemia indica named 'Pingning Xiang', and can improve the rooting rate of the lagerstroemia indica cutting propagation and shorten the time of the lagerstroemia indica cutting propagation by adding the step of effectively removing the cutting shoot inhibitor of the lagerstroemia indica and slowly applying the rooting agent in the cutting propagation process to continuously promote the rooting of the cutting shoot and adding the process of promoting the cell division and the release of the lateral bud growth substance in the cutting propagation process.
Description
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a crape myrtle hardwood cutting propagation method.
Background
Lagerstroemia indica L, also known as Mantang Red, whooping Red, pruritic Tree, etc., is a plant of Lagerstroemia genus of Lythraceae family. In hot summer, in the correct period of lacking flowers, the flowers bloom like fire, and the flowers are durable and not decayed from summer to autumn, so that the flower is a famous small summer flower-watching tree in China and even in the world, is praised as 4 famous flowers in summer with lily, pomegranate and lotus, can be named as 'flower top in summer', and has high ornamental value. Crape myrtle is a native product of China, has been used for appreciation since the Tang dynasty, and has been cultivated for more than one thousand, five and hundred years. About 40 varieties of lagerstroemia indica mainly comprise lagerstroemia indica with purple flower color, lagerstroemia indica with pure white flower color, red osmunda japonica with pink flower color and green osmunda japonica with blue-purple flower color.
The 'Pingningxiang' lagerstroemia indica is a new lagerstroemia indica aromatic variety cultivated by the plant research institute of Chinese academy of sciences in Jiangsu province, and is characterized by pink purple color, fragrance and high ornamental value. Hardwood cutting is an important method for breeding lagerstroemia indica varieties, but the rooting rate also has great difference due to the genotype difference among the varieties. The existing crape myrtle cutting propagation method is only suitable for conventional crape myrtle varieties, and the problems of low rooting rate, poor seedling quality, high production cost and the like can occur when the existing cutting propagation method is adopted to carry out cutting propagation on the 'Pinngxiang' crape myrtle. Therefore, a need exists for developing a hardwood cutting propagation method suitable for lagerstroemia indica named 'Pinning Xiang'.
Disclosure of Invention
In view of the above, the invention provides a crape myrtle hardwood cutting propagation method, which is suitable for crape myrtle with 'Pingningxiang', and the method is characterized in that the step of effectively removing the crape myrtle cutting shoot inhibitor is added, the rooting agent is slowly applied in the cutting propagation process, the cutting shoot rooting is continuously promoted, the process of promoting cell division and releasing the lateral bud growth substance is also added in the cutting propagation process, the rooting rate of the crape myrtle cutting propagation can be improved, and the cutting propagation time of the crape myrtle is shortened.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a crape myrtle hardwood cutting propagation method, which comprises the following steps:
step 1) preparing cutting slips: cutting branches without diseases and insect pests from crape myrtle mother trees, cutting the branches into cutting slips with the length of 5-10 cm, and bundling the slips into a bundle;
step 2) removing cuttings inhibitors: soaking the cutting slips in a mixed aqueous solution of acetone and ethanol for 30-60 minutes, taking out and standing for 60-120 minutes, repeating soaking-standing for at least 2 times, and storing the cutting slips in fine sand for 20-40 days, wherein the fine sand comprises fine sand containing first weak-base anion exchange resin and first polar adsorption resin to obtain the treated cutting slips;
step 3) preparing a matrix: vermiculite, quartz sand, perlite and slag ash are mixed according to the following weight parts (1-3): (2-5): (2-4): (1-2), sterilizing by using a disinfectant, and adding 0.1-0.6 part by weight of a second polar adsorption resin soaked in an indolebutyric acid solution and 0.01-0.1 part by weight of a second weak base anion exchange resin soaked in a chlorogenic acid solution to obtain a matrix;
step 4), cuttage: dipping a 1.5-3.0 cm part of the base part of the cutting obtained in the step 2) in 75% alcohol for 10-20 seconds, then dipping in 100-300 mg/L rooting agent solution for 30-50 seconds, and then inserting the cutting into the substrate in the step 3) to an insertion depth of 50-60% of the length of the cutting;
step 5) supplement of an accelerator: uniformly spreading third polar adsorption resin on the surface of the substrate on the 30 th to 40 th days of cuttage, wherein the third polar adsorption resin comprises polar adsorption resin with zeatin adsorbed thereon, and the spreading amount of the third polar adsorption resin is 0.05-0.1 part by weight;
step 6), hardening seedlings: and (5) when the rooting rate of the cutting slips reaches 70% -80%, training seedlings.
Preferably, the shoots of step 1) comprise annual and biennial shoots.
Preferably, the acetone and ethanol mixed aqueous solution in the step 2) has the acetone mass concentration of 3-5% and the ethanol mass concentration of 3.6-7.3%, and the water comprises high-purity water subjected to high-temperature sterilization.
Preferably, the first weak base anion exchange resin of step 2) comprises an hydroxide radical type weak base anion exchange resin, and the first polar adsorption resin comprises a polar adsorption resin having phenolic hydroxyl groups.
Preferably, the step 2) further comprises periodically introducing sterile gas into the fine sand to displace the gas therein.
Preferably, the concentration of the indolebutyric acid solution used for soaking the second polar adsorption resin in the step 3) is 450 mg/L-890 mg/L, and the concentration of the chlorogenic acid solution used for soaking the second weak base anion exchange resin is 230 mg/L-470 mg/L.
Preferably, the rooting agent solution in the step 4) comprises a mixed solution of indoleacetic acid and indolebutyric acid, wherein the mass ratio of the indoleacetic acid to the indolebutyric acid is (1-2): (1.5 to 3).
Preferably, the polar adsorption resin adsorbed with the zeatin in the step 5) comprises a polar resin soaked in a solution with the zeatin concentration of 80 mg/L-120 mg/L, and the zeatin solution is prepared by dissolving the zeatin in an ethanol solution with the mass concentration of 26% -41%.
Preferably, the seedling exercising time in the step 6) is 10-15 days.
Compared with the prior art, the invention has the following beneficial technical effects: firstly, before cutting, the process of soaking the cutting slips in solution and sand storage of the cutting slips added with an adsorbent is adopted to leach out inhibitors in the cutting slips, so that the rooting rate of the cutting slips is improved and the rooting speed is accelerated; secondly, the rooting agent is slowly applied in the cutting propagation process, so that the rooting of the cutting slips is continuously promoted, and the rooting speed of the cutting slips is accelerated; and thirdly, in the process of cutting propagation, the process of promoting cell division and releasing lateral bud growth substances is added, the rooting rate of cutting propagation of the lagerstroemia indica is improved, and the time of cutting propagation of the lagerstroemia indica is shortened.
Detailed Description
The technical solution of the present invention will be described in detail with reference to the following examples.
It should be noted that, in the case of no conflict, the features in the following embodiments and examples may be combined with each other; and all other embodiments obtained by those of ordinary skill in the art without making any inventive step are intended to be within the scope of the present disclosure.
It is noted that various aspects of the embodiments are described below within the scope of the appended claims. It should be apparent that the aspects described herein may be embodied in a wide variety of forms and that any specific structure and/or function described herein is merely illustrative. Based on the disclosure, one skilled in the art should appreciate that one aspect described herein may be implemented independently of any other aspects and that two or more of these aspects may be combined in various ways. For example, an apparatus may be implemented and/or a method practiced using any number of the aspects set forth herein.
It should be noted that the ordinal numbers such as "first", "second", "third", etc., used herein merely distinguish between the items being described and do not have any sequential or technical meaning.
The method is suitable for the lagerstroemia indica 'Pinning fragrance', can continuously promote the cutting shoot to root by adding the step of effectively removing the cutting shoot inhibitor of the lagerstroemia indica and slowly applying the rooting agent in the cutting propagation process, can also increase the process of promoting cell division and the release of lateral bud growth substances in the cutting propagation process, can improve the rooting rate of the lagerstroemia indica cutting propagation and shorten the cutting propagation time of the lagerstroemia indica.
The invention provides a crape myrtle hardwood cutting propagation method, which comprises the following steps:
step 1) preparing cutting slips: cutting branches without diseases and insect pests from the lagerstroemia indica mother tree, cutting the branches into cuttings with the length of 5-10 cm, and bundling the cuttings into a bundle;
step 2) removing cuttings inhibitors: soaking the cutting slips in a mixed aqueous solution of acetone and ethanol for 30-60 minutes, taking out and standing for 60-120 minutes, repeating soaking-standing for at least 2 times, and storing the cutting slips in fine sand for 20-40 days, wherein the fine sand comprises fine sand containing first weak base anion exchange resin and first polarity adsorption resin to obtain the treated cutting slips;
step 3) preparation of the matrix: vermiculite, quartz sand, perlite and slag ash are mixed according to the following weight parts (1-3): (2-5): (2-4): (1-2), sterilizing by using a disinfectant, and adding 0.1-0.6 part by weight of a second polar adsorption resin soaked in an indolebutyric acid solution and 0.01-0.1 part by weight of a second weak base anion exchange resin soaked in a chlorogenic acid solution to obtain a matrix;
step 4), cuttage: dipping 1.5-3.0 cm of the base part of the cutting obtained in the step 2) in 75% alcohol for 10-20 seconds, then dipping in 100-300 mg/L rooting agent solution for 30-50 seconds, and then inserting the cutting into the substrate in the step 3) to an insertion depth of 50-60% of the length of the cutting;
step 5) supplement of an accelerator: uniformly spreading third polar adsorption resin on the surface of the substrate on the 30 th to 40 th days of cuttage, wherein the third polar adsorption resin comprises polar adsorption resin absorbed with zeatin, and the spreading amount of the third polar adsorption resin is 0.05-0.1 part by weight;
step 6), hardening seedlings: and (5) when the rooting rate of the cutting slips reaches 70% -80%, training seedlings.
Preferably, the shoots of step 1) comprise annual and biennial shoots.
Further preferably, the shoots of step 1) comprise two year old shoots.
Preferably, the acetone mass concentration of the acetone and ethanol mixed water solution in the step 2) is 3-5%, the ethanol mass concentration is 3.6-7.3%, and the water comprises high-purity water subjected to high-temperature sterilization.
In some embodiments, the acetone mass concentration of the acetone and ethanol mixed aqueous solution may be 3.5%, 4.0%, 4.5%, 5.0%; the mass concentration of ethanol may be 3.6%, 4.0%, 4.3%, 4.6%, 5.1%, 5.5%, 5.8%, 6.2%, 6.7%, 7.0%, 7.3%.
In some embodiments, the time for soaking the cutting in the mixed aqueous solution of acetone and ethanol may be 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, 60 minutes; the time for taking out the mixed water solution of acetone and ethanol and standing can be 60 minutes, 70 minutes, 80 minutes, 90 minutes, 100 minutes, 110 minutes and 120 minutes; the number of repeated soaking-standing may be 2 times, 3 times, 4 times, 5 times, etc.
Further preferably, the temperature of the cutting soaked in the mixed aqueous solution of acetone and ethanol is 28-34 ℃, and in the temperature range, the biological activity of the cutting can be maintained, and inhibitors in the cutting can be diffused out as soon as possible.
Further preferably, the step of taking out the cutting slips from the acetone and ethanol mixed water solution and standing is to stand the cutting slips in a sterile environment, the temperature is kept between 20 ℃ and 28 ℃, the relative humidity is kept between 76 percent and 88 percent, and the oxygen content in the environment gas is between 28 percent and 36 percent. The oxygen content of the environmental gas is higher than that of the conventional air, so that the oxygen deficiency and activity reduction degree of the cutting slips caused by soaking can be relieved, and the biological activity of the cutting slips can be ensured on the premise of effectively ensuring the removal rate of inhibitors.
In some embodiments, the cuttings are stored in fine sand, i.e., the sand may be stored for 20, 25, 30, 35, 40 days.
Further preferably, the sand storage temperature is 12-18 ℃, and the humidity of the sand layer is 65-75%, so that the cuttings can not germinate and lose water to cause inactivation.
Further preferably, the diameter of the fine sand grains is 0.125-0.25 mm.
Preferably, the first weak base anion exchange resin of step 2) comprises an hydroxide radical type weak base anion exchange resin and the first polar adsorption resin comprises a polar adsorption resin having phenolic hydroxyl groups.
Further preferably, the first weakly basic anion exchange resin comprises at least one of the designations D301, D306, D308. The method has the function of adsorbing or exchanging the inhibitors discharged from the cutting in the sand storage process, such as phthalic acid, catechin, abscisic acid and the like, into the first weak-base anion exchange resin, so that the concentration of the inhibitors in the sand storage environment is reduced, and the inhibitors can be discharged from the cutting more quickly.
D301, D306, and D308 are available from Jiangsu Suqing group.
Further preferably, the polar adsorption resin having phenolic hydroxyl groups includes an NKA-2 adsorption resin. The effect of the method is to adsorb inhibitors discharged from the cutting slips in the sand storage process, such as phthalic acid, catechin, abscisic acid, ethylene and other organic matters onto polar resin, reduce the concentration of the inhibitors in the sand storage environment and facilitate the inhibitors to be discharged from the cutting slips more quickly.
The ethanol and acetone in the mixed solution can increase the permeability of cell walls and cell membranes of cells at the positions of cuttings, barks and the like, so that the discharge speed of inhibitors is increased. Most of inhibitors in the cutting can be removed in the process of multiple soaking, the concentration of the inhibitors in the cutting is relatively low, if the method of continuous soaking is adopted, not only the leaching rate of the inhibitors is reduced, but also the risk of activity reduction of the cutting caused by soaking is increased, and therefore, the inhibitors in the cutting are further reduced by adopting a sand storage mode. The weakly alkaline ion exchange resin and the polar adsorption resin are added into the sand storage, so that the inhibitor discharged from the cutting can be adsorbed in time, the concentration of the free inhibitor in the sand storage environment is reduced, the concentration difference of the inhibitor between the cutting and the environment is increased, and the inhibitor discharge can be accelerated.
Preferably, step 2) further comprises periodically introducing sterile gas into the fine sand to displace the gas therein.
Further preferably, the sterile gas comprises air subjected to microfiltration sterilization, and the flow rate of the sterile gas introduced into the fine sand from the bottom is 1-2 cubic meters per square meter per hour.
Preferably, the concentration of the indolebutyric acid solution used for soaking the second polar adsorption resin in the step 3) is 450 mg/L-890 mg/L, and the concentration of the chlorogenic acid solution used for soaking the second weak base anion exchange resin is 230 mg/L-470 mg/L.
In some embodiments, the concentration of the solution of indolebutyric acid used to soak the second polar adsorbent resin may be 450mg/L, 500mg/L, 550mg/L, 600mg/L, 650mg/L, 700mg/L, 750mg/L, 800mg/L, 850mg/L, 890 mg/L.
In some embodiments, the concentration of the chlorogenic acid solution used to soak the second weakly basic anion exchange resin may be 230mg/L, 260mg/L, 300mg/L, 340mg/L, 390mg/L, 410mg/L, 440mg/L, 470 mg/L.
Further preferably, the second polar adsorption resin comprises at least one of NKA-9, NKA-2 and AB-8. NKA-9, NKA-2 and AB-8 resins were pretreated by a method known in the art. NKA-9, NKA-2, AB-8 were purchased from the university of Nankai chemical plant.
The pretreated wet second polarity adsorption resin without free water is soaked in the indolebutyric acid solution, and the mass ratio of the wet mass of the second polarity adsorption resin to the indolebutyric acid solution is 1: (5-15), the soaking temperature is 10-20 ℃, the soaking time is 80-130 minutes, the second polar adsorption resin adsorbs the indolebutyric acid to achieve adsorption balance, and at the moment, the adsorption amount of the second polar adsorption resin on the indolebutyric acid reaches 40-50 mg/g wet resin. And the second polar adsorption resin achieving the adsorption balance of the indolebutyric acid is subjected to low-speed centrifugation, and the free indolebutyric acid solution is removed and then is sealed and stored for later use, so that complete drying is prevented, and the release amount and release speed of the subsequent indolebutyric acid are prevented from being influenced.
The preparation of the indolebutyric acid solution adopts the method in the prior art, wherein the mass content of ethanol for dissolving indolebutyric acid in the solution is less than 1 percent so as to increase the adsorption quantity of indolebutyric acid on polar resin.
Further preferably, the second weakly basic anion exchange resin comprises at least one of 313, 316, D318. 313. The 316 and D318 resins are pretreated by the method of the prior art. 313. 316, D318 resins are available from the shochu group of Jiangsu.
The pretreated wet second weak base anion exchange resin without free water is soaked in the chlorogenic acid solution, and the mass ratio of the wet mass of the second weak base anion exchange resin to the chlorogenic acid solution is 1: (7-18), the soaking temperature is 16-26 ℃, the soaking time is 30-90 minutes, the chlorogenic acid adsorbed by the second weak base anion exchange resin reaches adsorption balance, and the adsorption capacity of the second weak base anion exchange resin to the chlorogenic acid reaches 80-100 mg/g wet resin. The second polarity adsorption resin which achieves the adsorption balance of the chlorogenic acid is subjected to low-speed centrifugation, free chlorogenic acid solution is removed, and then the second polarity adsorption resin is sealed and stored for later use, so that complete drying is prevented, and the release amount and release speed of the subsequent chlorogenic acid are prevented from being influenced.
Further preferably, mixing vermiculite, quartz sand, perlite and slag ash in the matrix in the step 3), and then soaking and disinfecting by using 0.3-0.6% potassium permanganate solution, wherein the soaking time is 5-10 minutes, and the temperature is normal temperature; after the soaking is finished, the potassium permanganate solution is drained, and is washed for 2-4 times by using sterilized deionized water, potassium permanganate remained on the matrix is washed clean, and the residual potassium permanganate is prevented from influencing the rooting rate and rooting speed of the cutting slips.
Preferably, the rooting agent solution in the step 4) comprises a mixed solution of indoleacetic acid and indolebutyric acid, wherein the mass ratio of the indoleacetic acid to the indolebutyric acid is (1-2): (1.5 to 3). In some embodiments, the mass ratio of indoleacetic acid to indolebutyric acid can be any value between 1:3 and 4:3, for example, 1:3, 2:3, 1:1, 4: 3.
In some embodiments, the concentration of the rooting agent solution of step 4) can be 100mg/L, 150mg/L, 200mg/L, 250mg/L, 300mg/L, and the time for dipping the cutting in the rooting agent solution can be 30 seconds, 35 seconds, 40 seconds, 45 seconds, 50 seconds.
Further preferably, the cuttage is carried out in a light-permeable room, such as a greenhouse, and the relative humidity of the air in the inside of the greenhouse is 80% -90%. After cuttage, the substrate is thoroughly poured by water, then water is automatically sprayed for supplying water, the water is sprayed for 30 seconds every 60 minutes in a sunny day, the water is sprayed for 20 seconds every 90 minutes in a cloudy day, and the water spraying is stopped at night. The water supply amount of the automatic spray water supply is 0.1-1L/square meter.h, wherein the area is the surface area of the substrate.
Further preferably, the light transmittance in the light-permeable chamber varies throughout the cutting process, with the overall tendency to decrease in shade. In a specific embodiment, the light shading rate is 70-80% in the early stage of cutting, such as from day 1 to day 20 of cutting; in the middle stage of cutting, such as 21 st to 30 th days of cutting, the shading rate is 65-75 percent; in the later stage of cutting, such as 31 days to 40 days of cutting, the shading rate is 55 percent to 65 percent; and after the later stage of cuttage, gradually removing the shading materials.
It should be noted that the early stage of cutting is a rooting stage, and the bud rising of the cutting shoot is avoided as much as possible; in the middle stage of cuttage, the root growth and thickening stage are performed, and in the lateral bud induction growth stage, the illumination needs to be increased to a certain degree; the later stage of the cuttage is a lateral bud and cutting shoot leaf growth stage, and the illumination should be further increased.
Watering and spraying water for 5 days before cuttage, wherein the water is purified well water, river water, municipal water and other natural water bodies; the water sprayed to the water from the 6 th to the 12 th days of cuttage is a dilute solution of lactic acid, wherein the concentration of the lactic acid is 10 mg/L-50 mg/L; the water sprayed to the water after 13 days of cuttage is dilute solution containing lactic acid and uric acid, wherein the concentration of the lactic acid is still 10 mg/L-50 mg/L, and the concentration of the urea is 100 mg/L-300 mg/L.
It should be noted that the lactic acid in the spray feed water acts as follows: on one hand, the indolebutyric acid adsorbed on the second polar adsorption resin, the chlorogenic acid on the second weak base anion exchange resin and the zeatin on the third polar adsorption resin are gradually desorbed, so that the rooting of the cutting shoot can be continuously promoted, or the germination of the cutting shoot can be promoted; on the other hand, the lactic acid also has a certain bacteriostatic action, can inhibit the growth of mixed bacteria and improve the survival rate of the cutting.
The effect of urea in the spray feed water is: after partial roots of the cutting slips are generated, nutrition is provided for further rooting and germination of the cutting slips.
Preferably, the polar adsorption resin adsorbed with the zeatin in the step 5) comprises a polar resin soaked in a solution with the zeatin concentration of 80 mg/L-120 mg/L, and the zeatin solution is prepared by dissolving the zeatin in an ethanol solution with the mass concentration of 26% -41%.
In some embodiments, the concentration of zeatin in the solution that is soaked with the third polar adsorbent resin may be 80mg/L, 90mg/L, 100mg/L, 110mg/L, 120 mg/L. The concentration of ethanol in the ethanol solution in which zeatin is dissolved may be 26%, 30%, 33%, 38%, 41%.
Further preferably, the third polar absorbent resin comprises at least one of DA201-M8, DA201-M, DA 201-H. The DA201-M8 and DA201-M, DA201-H resins are pretreated by the method in the prior art. DA201-M8, DA201-M, DA201-H resins are available from Jiangsu Suqing group.
The third polar adsorption resin in a wet state, which is pretreated and free water is removed, is soaked in the ethanol solution of the zeatin, and the mass ratio of the wet mass of the third polar adsorption resin to the mass of the zeatin ethanol is 1: (8-23), the soaking temperature is 5-10 ℃, the soaking time is 120-160 minutes, the third polar adsorption resin adsorbs the zeatin to achieve adsorption balance, and at the moment, the adsorption amount of the third polar adsorption resin on the zeatin reaches 24-43 mg/g wet resin. The third polar adsorption resin achieving the zeatin adsorption balance is subjected to low-speed centrifugation, and is sealed and stored for later use after a free zeatin ethanol solution is removed, so that complete drying is prevented, and the release amount and release speed of subsequent zeatin are prevented from being influenced.
It should be noted that zeatin acts to promote cell division and lateral bud growth of the cutting after the cutting begins to sprout.
Preferably, the seedling exercising time in the step 6) is 10-15 days.
For a further understanding of the present invention, reference will now be made to the following descriptions taken in conjunction with the accompanying examples, and it is to be understood that the descriptions are intended to illustrate further features and advantages of the invention, rather than to limit the scope of the appended claims.
Examples 1 to 6
According to the following method, the lagerstroemia indica of the Pinning-fragrance variety is subjected to hardwood cutting propagation:
step 1) preparing cutting slips: cutting branches without diseases and insect pests from the lagerstroemia indica trees with the length and the growth period of the branches as shown in table 1;
step 2) removing cuttings inhibitors: soaking the cutting slips in a mixed aqueous solution of acetone and ethanol, taking out and standing, repeatedly soaking and standing for multiple times, and then storing fine sand containing fine sand of a first weak-base anion exchange resin and a first polar adsorption resin in the fine sand to obtain the treated cutting slips, wherein the water comprises high-temperature sterilized high-purity water, the standing is carried out in an aseptic environment, the diameter of fine sand grains is 0.125-0.25 mm, the first polar adsorption resin is NKA-2 resin, the aeration is carried out in the sand storage process to sterile air, the soaking time, the soaking temperature, the standing time, the standing temperature, the standing environment humidity, the oxygen content of the standing environment, the soaking-standing times, the acetone mass concentration, the ethanol mass concentration, the sand storage time, the sand storage temperature, the sand layer humidity and the first weak-base anion exchange resin brand in the mixed aqueous solution of acetone and ethanol, The ventilation amount and other parameters in sand storage are shown in table 2;
step 3) preparing a matrix: mixing vermiculite, quartz sand, perlite and furnace slag ash according to a certain weight part, sterilizing by using a disinfectant, adding a second polar adsorption resin soaked by an indolebutyric acid solution and a second weak base anion exchange resin soaked by a chlorogenic acid solution to obtain a matrix, pretreating the second polar adsorption resin and the second weak base anion exchange resin by adopting the method in the prior art, soaking and sterilizing the matrix by using a potassium permanganate solution at normal temperature, draining the potassium permanganate solution after soaking, washing by using sterilized deionized water, and mixing the vermiculite, the quartz sand, the perlite and the furnace slag ash according to the proportion, the addition amount of the second polar adsorption resin, the addition amount of the second weak base anion exchange resin, the concentration of the indolebutyric acid solution for soaking the second polar adsorption resin, the concentration of the chlorogenic acid solution for soaking the second weak base anion exchange resin, and the reaction of the second weak base anion exchange resin, Parameters such as the grade of the second polar adsorption resin, the grade of the second weak-base anion exchange resin, the concentration of the potassium permanganate solution, the soaking time of the potassium permanganate solution, the washing times of the sterilized deionized water and the like are shown in table 3;
step 4), cuttage: dipping the 1.5-3.0 cm part of the base part of the cutting shoot obtained in the step 2) in 75% alcohol for a certain time, then dipping in a rooting agent solution for a certain time, inserting the cutting slips into the substrate in the step 3), wherein the rooting agent solution is a mixed solution of indoleacetic acid and indolebutyric acid, the cutting is carried out in a light-permeable room, water is automatically sprayed in the cutting process, 30 seconds are sprayed every 60 minutes in a sunny day, 20 seconds are sprayed every 90 minutes in a cloudy day, the water spraying is stopped at night, the shading rate is gradually reduced in the cutting process, the insertion depth is 50% -60% of the cutting slip length, and the parameters of alcohol dipping time, rooting agent solution concentration, rooting agent solution dipping time, the mass ratio of indoleacetic acid and indolebutyric acid in the rooting agent, indoor air relative humidity of the cutting, spraying water supply quantity, the shading rate in the early stage of the cutting, the shading rate in the middle stage of the cutting, the shading rate in the later stage of the cutting and the like are shown in a table 4;
step 5) supplement of an accelerator: in the cutting process, third polar adsorption resin is uniformly spread on the surface of the substrate, the third polar adsorption resin comprises polar adsorption resin absorbed with zeatin and is soaked by an ethanol solution of the zeatin, the third polar adsorption resin is pretreated by adopting a method in the prior art before being used, and parameters such as the time for supplementing the accelerant, the spreading amount of the third polar adsorption resin, the concentration of the zeatin soaked in the third polar adsorption resin, the ethanol content in the zeatin solution, the brand number of the third polar adsorption resin and the like are shown in table 5;
step 6), hardening seedlings: and when the rooting rate of the cutting shoots reaches 70-80%, training seedlings. Preferably, the seedling exercising time in the step 6) is 10-15 days.
TABLE 1 Lagerstroemia indica hard wood cutting propagation method of the present invention
TABLE 2 Lagerstroemia indica hard wood cutting propagation method part of the parameters
TABLE 3 Lagerstroemia indica hard wood cutting propagation method part of the parameters
TABLE 4 Lagerstroemia indica hard wood cutting propagation method part of the parameters
TABLE 5 Lagerstroemia indica hard wood cutting propagation method part of the parameters
The parameters of the sample collection starting rooting time, the average root number, the average root length, the rooting rate, the lateral bud starting growth time and the like of the examples 1 to 6 are collected, and the collection method of each parameter is the method in the prior art. And the statistical node of the average root number, the average root length and the rooting rate is the 50 th day of the beginning of cuttage. The crape myrtle hardwood cutting effect parameters are shown in table 6.
TABLE 6 Effect parameters of the hard branch cutting propagation method of crape myrtle of the present invention
Comparative example 1
According to the parameters of the crape myrtle hardwood cutting propagation method in the embodiment 3, but the step 2) and the step 5) are omitted, the second polar adsorption resin and the second weak-base anion exchange resin are not added into the substrate in the step 3), and the rooting agent in the step 4) is indolebutyric acid solution. The detection and the measurement result show that the crape myrtle cuttage rooting starting time of the comparative example is 41 days, the average number of roots is 7, the average root length is 8.5 cm, the rooting rate is 82%, and the lateral bud growth starting time is 61 days.
In conclusion, compared with the cutting method in the prior art, the crape myrtle hardwood cutting propagation method has the advantages that the rooting starting time is advanced by more than 9 days, the average root number is improved by more than 50%, the average root length is improved by more than 24%, the rooting rate is improved by more than 15%, and the side bud growth starting time is advanced by more than 10 days.
Claims (9)
1. The crape myrtle hardwood cutting propagation method is characterized by comprising the following steps:
step 1) preparing cutting slips: cutting branches without diseases and insect pests from a crape myrtle mother tree, cutting the branches into cuttings with the length of 5-10 cm, and bundling the cuttings into a bundle;
step 2) removing cuttings inhibitors: soaking the cutting slips in a mixed water solution of acetone and ethanol for 30-60 minutes, taking out and standing for 60-120 minutes, repeating soaking-standing for at least 2 times, and then performing fine sand storage treatment on the cutting slips for 20-40 days, wherein the fine sand comprises fine sand containing a first weak base anion exchange resin and a first polar adsorption resin, the taking out and standing of the cutting slips from the mixed water solution of acetone and ethanol is performed in a sterile environment, the temperature is kept between 20 ℃ and 28 ℃, the relative humidity is kept between 76% and 88%, and the oxygen content in the environment gas is between 28% and 36%, so that the treated cutting slips are obtained;
step 3) preparation of the matrix: vermiculite, quartz sand, perlite and slag ash are mixed according to the following weight parts (1-3): (2-5): (2-4): (1-2), sterilizing by using a disinfectant, and adding 0.1-0.6 part by weight of a second polar adsorption resin soaked in an indolebutyric acid solution and 0.01-0.1 part by weight of a second weak base anion exchange resin soaked in a chlorogenic acid solution to obtain a matrix;
step 4), cuttage: dipping 1.5-3.0 cm of the base part of the treated cutting shoot obtained in the step 2) in 75% alcohol for 10-20 seconds, then dipping in 100-300 mg/L rooting agent solution for 30-50 seconds, then inserting the cutting shoot into the substrate in the step 3), wherein the insertion depth is 50-60% of the length of the cutting shoot, and the water for watering and spraying water in the first 5 days of cutting is purified well water, river water or municipal water; spraying water from 6 th to 12 th days of cuttage to obtain dilute solution of lactic acid, wherein the concentration of the lactic acid is 10-50 mg/L; the water sprayed to the water after 13 days of cuttage is a dilute solution containing lactic acid and uric acid, wherein the concentration of the lactic acid is still 10-50 mg/L, and the concentration of urea is 100-300 mg/L;
step 5) supplement of an accelerator: uniformly spreading third polar adsorption resin on the surface of the substrate in 30 th to 40 th days of cuttage, wherein the third polar adsorption resin comprises polar adsorption resin with zeatin adsorbed thereon, and the spreading amount of the third polar adsorption resin is 0.05-0.1 part by weight;
step 6), hardening seedlings: and training seedlings when the rooting rate of the cuttings reaches 70% -80%.
2. The method for cutting propagation of crape myrtle branches according to claim 1, wherein the branches in step 1) comprise annual branches and biennial branches.
3. The method for cutting propagation of crape myrtle branches according to claim 1, wherein the acetone and ethanol mixed aqueous solution in the step 2) has an acetone mass concentration of 3% -5% and an ethanol mass concentration of 3.6% -7.3%, and the water comprises high-purity water subjected to high-temperature sterilization.
4. The method for cutting propagation of crape myrtle branches according to claim 1, wherein the first weak base anion exchange resin in step 2) comprises an oxyhydrogen base weak base anion exchange resin, and the first polar adsorption resin comprises a polar adsorption resin with phenolic hydroxyl groups.
5. The method for cutting propagation of crape myrtle branches according to claim 1, wherein the step 2) further comprises periodically introducing sterile gas into the fine sand to replace the gas therein.
6. The method for cutting propagation of crape myrtle branches according to claim 1, wherein the concentration of the indolebutyric acid solution used for soaking the second polar adsorption resin in the step 3) is 450 mg/L-890 mg/L, and the concentration of the chlorogenic acid solution used for soaking the second weak-base anion exchange resin is 230 mg/L-470 mg/L.
7. The crape myrtle hardwood cutting propagation method according to claim 1, wherein the rooting agent solution in the step 4) comprises a mixed solution of indoleacetic acid and indolebutyric acid, and the mass ratio of the indoleacetic acid to the indolebutyric acid is (1-2): (1.5 to 3).
8. The method for cutting propagation of crape myrtle branches according to claim 1, wherein the polar adsorption resin adsorbed with zeatin in step 5) comprises a polar resin soaked in a solution with zeatin concentration of 80-120 mg/L, and the zeatin solution is prepared by dissolving zeatin in an ethanol solution with mass concentration of 26-41%.
9. The crape myrtle hardwood cutting propagation method according to claim 1, wherein the seedling hardening time in step 6) is 10-15 days.
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