CN114277086A - Helicobacter pylori rapid detection reagent - Google Patents
Helicobacter pylori rapid detection reagent Download PDFInfo
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- CN114277086A CN114277086A CN202111623987.9A CN202111623987A CN114277086A CN 114277086 A CN114277086 A CN 114277086A CN 202111623987 A CN202111623987 A CN 202111623987A CN 114277086 A CN114277086 A CN 114277086A
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Abstract
The invention belongs to the field of detection, in particular to a reagent for rapidly detecting helicobacter pylori, which aims at the problems of low test speed and poor test accuracy of the prior art, and provides the following scheme that the reagent comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and table salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, the helicobacter pylori is rapidly cultured by adopting an improved urease method and an international common ultra-rapid culture medium culture test, so that the helicobacter pylori rapidly secretes high-activity urease to decompose substrate urea to generate ammonia gas, the pH value of the ammonia gas is increased when the ammonia is dissolved in water, the color of the indicator is changed, and the helicobacter pylori in saliva, tartar or gastric mucosa tissues is indirectly stained to judge whether the helicobacter pylori exists or not, the test speed is high, and the accuracy is high.
Description
Technical Field
The invention relates to the technical field of detection, in particular to a reagent for rapidly detecting helicobacter pylori.
Background
The invention discloses a helicobacter pylori detection kit and a preparation method thereof, and discloses the helicobacter pylori detection kit and the preparation method thereof, the kit can be used for detecting the infection condition of helicobacter pylori in saliva, the reagent is simple, the cost is low, no harm, pain, toxic and side reaction and contraindication are caused to a human body, the operation is simple, convenient and rapid, the sensitivity is high, and the kit is suitable for popularization and application.
However, the helicobacter pylori detection kit and the preparation method thereof have some problems, such as slow detection speed, often large time spent on observation to find color change, complex operation steps, often need of operators to have extremely high test level, easy error and poor test accuracy.
Disclosure of Invention
Based on the technical problems of low testing speed and poor testing accuracy in the background technology, the invention provides a reagent for rapidly detecting helicobacter pylori.
The invention provides a reagent for rapidly detecting helicobacter pylori, which comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and table salt, and the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide.
Preferably, the internal components of the detection reagent comprise 15 parts of sodium chloride, 10-14 parts of urea, 5-10 parts of indicator, 35-60 parts of ethanol solution, 5-10 parts of absorbent, 5-9 parts of additive and 35-60 parts of culture medium.
Preferably, the proportion of the niclosite, the plant ash and the salt in the absorbent is 4.5:2.5:3, and the proportion of the vinegar, the disodium hydrogen phosphate and the sodium hydroxide in the additive is 6:2.5: 1.5.
Preferably, the ethanol solution is used to dissolve sodium chloride, urea, indicators, absorbents and additives.
Preferably, the preparation method of the absorbent comprises the following steps: firstly, respectively grinding the bischofite and the salt, grinding the bischofite and the salt into powder of 50 meshes to obtain bischofite powder and salt powder, adding the bischofite powder into a reaction kettle, adding hard water with high salinity, controlling the rotating speed of the reaction kettle at 420 r/min-550 r/min, raising the temperature to 55-70 ℃, then stirring and reacting for 15-25 minutes, then adding the salt powder into the reaction kettle, raising the temperature to 75-80 ℃, putting the plant ash into a refrigerating chamber, controlling the refrigerating temperature to-45-15 ℃, controlling the refrigerating time to 1-3 days, and adding the cooled plant ash into a reaction kettle, increasing the rotating speed of the reaction kettle to 720 r/min-920 r/min, and stirring for reacting for 4-12 hours to obtain the absorbent.
Preferably, the preparation method of the additive comprises the following steps: firstly, cooling vinegar to-25 to-15 ℃, then adding disodium hydrogen phosphate and sodium hydroxide into a reaction kettle, controlling the rotating speed of the reaction kettle to be 220r/min to 350r/min, raising the temperature to 55 to 65 ℃, stirring for reaction for 15 to 25 minutes, then filling inert gas into the reaction kettle, then pouring the vinegar into the reaction kettle, continuously stirring for reaction for 15 to 25 minutes to obtain a mixed solution, pouring the mixed solution into drying and dehydrating equipment for evaporation until two thirds of the mixed solution is left, and thus obtaining the additive.
Preferably, the culture medium is one or more of BME culture medium, RPMI-1640 culture medium, DMEM culture medium, F10 culture medium, Fischer's culture medium and MEM culture medium.
Preferably, the indicator is a PH indicator, and the sodium chloride and the urea are both ground into powder.
Preferably, both the absorbent and the additive are decolorized by a decolorizing agent to be colorless.
Preferably, the test method of the detection reagent comprises: adding sodium chloride into an ethanol solution, stirring uniformly, adding urea and an indicator, continuing stirring, adding an absorbent and an additive, and stirring uniformly to obtain a test solution; dividing the culture medium into a bottom culture medium and a negative culture medium, respectively pouring the culture medium and the negative culture medium into two penicillin bottles, then equally dividing the prepared test solution and the test sample into two parts, respectively adding the two parts into the two penicillin bottles, observing the color change after stirring, and indicating that the test solution is positive if the color is yellow or purple, thereby indicating that the test solution contains the helicobacter pylori.
The invention has the beneficial effects that: the method adopts an improved urease method and adopts an international common ultra-fast culture medium culture test to rapidly culture the helicobacter pylori, so that the helicobacter pylori can rapidly secrete high-activity urease to decompose substrate urea to generate ammonia gas, the pH value of the indicator is increased by dissolving the ammonia in water, the color of the indicator is changed, and the helicobacter pylori in saliva, tartar or gastric mucosa tissues is indirectly dyed to judge whether the helicobacter pylori exists.
Drawings
FIG. 1 is a schematic diagram of the detection process of a reagent for rapid detection of helicobacter pylori according to the present invention.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Referring to FIG. 1, the first embodiment
The embodiment provides a helicobacter pylori rapid detection reagent, which comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, the internal components of the detection reagent comprise 15 parts of sodium chloride, 14 parts of urea, 10 parts of the indicator, 60 parts of the ethanol solution, 10 parts of the absorbent, 9 parts of the additive and 60 parts of the culture medium, the ratio of the niclosite, the plant ash and the salt in the absorbent is 4.5:2.5:3, the ratio of the vinegar, the disodium hydrogen phosphate and the sodium hydroxide in the additive is 6:2.5:1.5, the ethanol solution is used for dissolving the sodium chloride, the urea, the indicator, the absorbent and the additive, and the preparation method of the absorbent comprises the following steps: grinding the niclosite and the salt respectively to obtain powder with 50 meshes, adding the niclosite powder into a reaction kettle, adding high-salinity hard water, controlling the rotating speed of the reaction kettle to be 420-550 r/min, raising the temperature to 55-70 ℃, stirring for reaction for 15-25 minutes, adding the salt powder into the reaction kettle, raising the temperature to be 75-80 ℃, placing the plant ash into a cold storage chamber, controlling the cold storage temperature to be-45-15 ℃ and the cold storage time to be 1-3 days, adding the cooled plant ash into the reaction kettle, raising the rotating speed of the reaction kettle to be 720-920 r/min, stirring for reaction for 4-12 hours to obtain the absorbent, the preparation method of the additive comprises the following steps: firstly, cooling vinegar to-25 to-15 ℃, then adding disodium hydrogen phosphate and sodium hydroxide into a reaction kettle, controlling the rotation speed of the reaction kettle to be 220 to 350r/min, raising the temperature to 55 to 65 ℃, stirring for reaction for 15 to 25 minutes, then filling inert gas into the reaction kettle, then pouring the vinegar into the reaction kettle, continuing to stir for reaction for 15 to 25 minutes to obtain a mixed solution, pouring the mixed solution into drying and dehydrating equipment for evaporation until two thirds of the mixed solution is left to obtain an additive, wherein the culture medium is one or more of a BME culture medium, an RPMI-1640 culture medium, a DMEM culture medium, an F10 culture medium, a Fischer's culture medium and a MEM culture medium, the indicator is a PH value indicator, sodium chloride and urea are ground to be powdery, and the absorbent and the additive are decolored by a decolorant, the preparation method of the detection reagent is as follows: adding sodium chloride into an ethanol solution, stirring uniformly, adding urea and an indicator, continuing stirring, adding an absorbent and an additive, and stirring uniformly to obtain a test solution; dividing the culture medium into a bottom culture medium and a negative culture medium, respectively pouring the culture medium and the negative culture medium into two penicillin bottles, then equally dividing the prepared test solution and the test sample into two parts, respectively adding the two parts into the two penicillin bottles, observing the color change after stirring, and indicating that the test solution is positive if the color is yellow or purple, thereby indicating that the test solution contains the helicobacter pylori.
Referring to FIG. 1, example II
The embodiment provides a helicobacter pylori rapid detection reagent, which comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, the internal components of the detection reagent comprise 15 parts of sodium chloride, 13 parts of urea, 7 parts of the indicator, 52 parts of the ethanol solution, 8 parts of the absorbent, 7 parts of the additive and 49 parts of the culture medium, the ratio of the niclosite, the plant ash and the salt in the absorbent is 4.5:2.5:3, the ratio of the vinegar, the disodium hydrogen phosphate and the sodium hydroxide in the additive is 6:2.5:1.5, the ethanol solution is used for dissolving the sodium chloride, the urea, the indicator, the absorbent and the additive, and the preparation method of the absorbent comprises the following steps: grinding the niclosite and the salt respectively to obtain powder with 50 meshes, adding the niclosite powder into a reaction kettle, adding high-salinity hard water, controlling the rotating speed of the reaction kettle to be 420-550 r/min, raising the temperature to 55-70 ℃, stirring for reaction for 15-25 minutes, adding the salt powder into the reaction kettle, raising the temperature to be 75-80 ℃, placing the plant ash into a cold storage chamber, controlling the cold storage temperature to be-45-15 ℃ and the cold storage time to be 1-3 days, adding the cooled plant ash into the reaction kettle, raising the rotating speed of the reaction kettle to be 720-920 r/min, stirring for reaction for 4-12 hours to obtain the absorbent, the preparation method of the additive comprises the following steps: firstly, cooling vinegar to-25 to-15 ℃, then adding disodium hydrogen phosphate and sodium hydroxide into a reaction kettle, controlling the rotation speed of the reaction kettle to be 220 to 350r/min, raising the temperature to 55 to 65 ℃, stirring for reaction for 15 to 25 minutes, then filling inert gas into the reaction kettle, then pouring the vinegar into the reaction kettle, continuing to stir for reaction for 15 to 25 minutes to obtain a mixed solution, pouring the mixed solution into drying and dehydrating equipment for evaporation until two thirds of the mixed solution is left to obtain an additive, wherein the culture medium is one or more of a BME culture medium, an RPMI-1640 culture medium, a DMEM culture medium, an F10 culture medium, a Fischer's culture medium and a MEM culture medium, the indicator is a PH value indicator, sodium chloride and urea are ground to be powdery, and the absorbent and the additive are decolored by a decolorant, the preparation method of the detection reagent is as follows: adding sodium chloride into an ethanol solution, stirring uniformly, adding urea and an indicator, continuing stirring, adding an absorbent and an additive, and stirring uniformly to obtain a test solution; dividing the culture medium into a bottom culture medium and a negative culture medium, respectively pouring the culture medium and the negative culture medium into two penicillin bottles, then equally dividing the prepared test solution and the test sample into two parts, respectively adding the two parts into the two penicillin bottles, observing the color change after stirring, and indicating that the test solution is positive if the color is yellow or purple, thereby indicating that the test solution contains the helicobacter pylori.
Referring to FIG. 1, example III
The embodiment provides a helicobacter pylori rapid detection reagent, which comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, the internal components of the detection reagent comprise 15 parts of sodium chloride, 12 parts of urea, 8 parts of the indicator, 51 parts of the ethanol solution, 9 parts of the absorbent, 8 parts of the additive and 41 parts of the culture medium, the ratio of the niclosite, the plant ash and the salt in the absorbent is 4.5:2.5:3, the ratio of the vinegar, the disodium hydrogen phosphate and the sodium hydroxide in the additive is 6:2.5:1.5, the ethanol solution is used for dissolving the sodium chloride, the urea, the indicator, the absorbent and the additive, and the preparation method of the absorbent comprises the following steps: grinding the niclosite and the salt respectively to obtain powder with 50 meshes, adding the niclosite powder into a reaction kettle, adding high-salinity hard water, controlling the rotating speed of the reaction kettle to be 420-550 r/min, raising the temperature to 55-70 ℃, stirring for reaction for 15-25 minutes, adding the salt powder into the reaction kettle, raising the temperature to be 75-80 ℃, placing the plant ash into a cold storage chamber, controlling the cold storage temperature to be-45-15 ℃ and the cold storage time to be 1-3 days, adding the cooled plant ash into the reaction kettle, raising the rotating speed of the reaction kettle to be 720-920 r/min, stirring for reaction for 4-12 hours to obtain the absorbent, the preparation method of the additive comprises the following steps: firstly, cooling vinegar to-25 to-15 ℃, then adding disodium hydrogen phosphate and sodium hydroxide into a reaction kettle, controlling the rotation speed of the reaction kettle to be 220 to 350r/min, raising the temperature to 55 to 65 ℃, stirring for reaction for 15 to 25 minutes, then filling inert gas into the reaction kettle, then pouring the vinegar into the reaction kettle, continuing to stir for reaction for 15 to 25 minutes to obtain a mixed solution, pouring the mixed solution into drying and dehydrating equipment for evaporation until two thirds of the mixed solution is left to obtain an additive, wherein the culture medium is one or more of a BME culture medium, an RPMI-1640 culture medium, a DMEM culture medium, an F10 culture medium, a Fischer's culture medium and a MEM culture medium, the indicator is a PH value indicator, sodium chloride and urea are ground to be powdery, and the absorbent and the additive are decolored by a decolorant, the preparation method of the detection reagent is as follows: adding sodium chloride into an ethanol solution, stirring uniformly, adding urea and an indicator, continuing stirring, adding an absorbent and an additive, and stirring uniformly to obtain a test solution; dividing the culture medium into a bottom culture medium and a negative culture medium, respectively pouring the culture medium and the negative culture medium into two penicillin bottles, then equally dividing the prepared test solution and the test sample into two parts, respectively adding the two parts into the two penicillin bottles, observing the color change after stirring, and indicating that the test solution is positive if the color is yellow or purple, thereby indicating that the test solution contains the helicobacter pylori.
Referring to FIG. 1, example No. four
The embodiment provides a helicobacter pylori rapid detection reagent, which comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, the internal components of the detection reagent comprise 15 parts of sodium chloride, 12 parts of urea, 6 parts of the indicator, 39 parts of the ethanol solution, 7 parts of the absorbent, 6 parts of the additive and 51 parts of the culture medium, the ratio of the niclosite, the plant ash and the salt in the absorbent is 4.5:2.5:3, the ratio of the vinegar, the disodium hydrogen phosphate and the sodium hydroxide in the additive is 6:2.5:1.5, the ethanol solution is used for dissolving the sodium chloride, the urea, the indicator, the absorbent and the additive, and the preparation method of the absorbent comprises the following steps: grinding the niclosite and the salt respectively to obtain powder with 50 meshes, adding the niclosite powder into a reaction kettle, adding high-salinity hard water, controlling the rotating speed of the reaction kettle to be 420-550 r/min, raising the temperature to 55-70 ℃, stirring for reaction for 15-25 minutes, adding the salt powder into the reaction kettle, raising the temperature to be 75-80 ℃, placing the plant ash into a cold storage chamber, controlling the cold storage temperature to be-45-15 ℃ and the cold storage time to be 1-3 days, adding the cooled plant ash into the reaction kettle, raising the rotating speed of the reaction kettle to be 720-920 r/min, stirring for reaction for 4-12 hours to obtain the absorbent, the preparation method of the additive comprises the following steps: firstly, cooling vinegar to-25 to-15 ℃, then adding disodium hydrogen phosphate and sodium hydroxide into a reaction kettle, controlling the rotation speed of the reaction kettle to be 220 to 350r/min, raising the temperature to 55 to 65 ℃, stirring for reaction for 15 to 25 minutes, then filling inert gas into the reaction kettle, then pouring the vinegar into the reaction kettle, continuing to stir for reaction for 15 to 25 minutes to obtain a mixed solution, pouring the mixed solution into drying and dehydrating equipment for evaporation until two thirds of the mixed solution is left to obtain an additive, wherein the culture medium is one or more of a BME culture medium, an RPMI-1640 culture medium, a DMEM culture medium, an F10 culture medium, a Fischer's culture medium and a MEM culture medium, the indicator is a PH value indicator, sodium chloride and urea are ground to be powdery, and the absorbent and the additive are decolored by a decolorant, the preparation method of the detection reagent is as follows: adding sodium chloride into an ethanol solution, stirring uniformly, adding urea and an indicator, continuing stirring, adding an absorbent and an additive, and stirring uniformly to obtain a test solution; dividing the culture medium into a bottom culture medium and a negative culture medium, respectively pouring the culture medium and the negative culture medium into two penicillin bottles, then equally dividing the prepared test solution and the test sample into two parts, respectively adding the two parts into the two penicillin bottles, observing the color change after stirring, and indicating that the test solution is positive if the color is yellow or purple, thereby indicating that the test solution contains the helicobacter pylori.
Referring to FIG. 1, example V
The embodiment provides a helicobacter pylori rapid detection reagent, which comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, the internal components of the detection reagent comprise 15 parts of sodium chloride, 10 parts of urea, 5 parts of the indicator, 35 parts of the ethanol solution, 5 parts of the absorbent, 5 parts of the additive and 35 parts of the culture medium, the ratio of the niclosite, the plant ash and the salt in the absorbent is 4.5:2.5:3, the ratio of the vinegar, the disodium hydrogen phosphate and the sodium hydroxide in the additive is 6:2.5:1.5, the ethanol solution is used for dissolving the sodium chloride, the urea, the indicator, the absorbent and the additive, and the preparation method of the absorbent comprises the following steps: grinding the niclosite and the salt respectively to obtain powder with 50 meshes, adding the niclosite powder into a reaction kettle, adding high-salinity hard water, controlling the rotating speed of the reaction kettle to be 420-550 r/min, raising the temperature to 55-70 ℃, stirring for reaction for 15-25 minutes, adding the salt powder into the reaction kettle, raising the temperature to be 75-80 ℃, placing the plant ash into a cold storage chamber, controlling the cold storage temperature to be-45-15 ℃ and the cold storage time to be 1-3 days, adding the cooled plant ash into the reaction kettle, raising the rotating speed of the reaction kettle to be 720-920 r/min, stirring for reaction for 4-12 hours to obtain the absorbent, the preparation method of the additive comprises the following steps: firstly, cooling vinegar to-25 to-15 ℃, then adding disodium hydrogen phosphate and sodium hydroxide into a reaction kettle, controlling the rotation speed of the reaction kettle to be 220 to 350r/min, raising the temperature to 55 to 65 ℃, stirring for reaction for 15 to 25 minutes, then filling inert gas into the reaction kettle, then pouring the vinegar into the reaction kettle, continuing to stir for reaction for 15 to 25 minutes to obtain a mixed solution, pouring the mixed solution into drying and dehydrating equipment for evaporation until two thirds of the mixed solution is left to obtain an additive, wherein the culture medium is one or more of a BME culture medium, an RPMI-1640 culture medium, a DMEM culture medium, an F10 culture medium, a Fischer's culture medium and a MEM culture medium, the indicator is a PH value indicator, sodium chloride and urea are ground to be powdery, and the absorbent and the additive are decolored by a decolorant, the preparation method of the detection reagent is as follows: adding sodium chloride into an ethanol solution, stirring uniformly, adding urea and an indicator, continuing stirring, adding an absorbent and an additive, and stirring uniformly to obtain a test solution; dividing the culture medium into a bottom culture medium and a negative culture medium, respectively pouring the culture medium and the negative culture medium into two penicillin bottles, then equally dividing the prepared test solution and the test sample into two parts, respectively adding the two parts into the two penicillin bottles, observing the color change after stirring, and indicating that the test solution is positive if the color is yellow or purple, thereby indicating that the test solution contains the helicobacter pylori.
Comparing the conventional detection reagent with the detection reagents prepared in examples one to five, the detection reagents prepared in examples one to five are as follows:
as can be seen from the above table, the speed and accuracy of the detection reagent prepared by the present invention are significantly improved, and the second embodiment is the best embodiment.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. The reagent for rapidly detecting the helicobacter pylori is characterized by comprising sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, wherein the absorbent comprises niclosite, plant ash and table salt, and the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide.
2. The reagent for rapidly detecting helicobacter pylori according to claim 1, wherein the internal components of the reagent are 15 parts of sodium chloride, 10-14 parts of urea, 5-10 parts of an indicator, 35-60 parts of an ethanol solution, 5-10 parts of an absorbent, 5-9 parts of an additive and 35-60 parts of a culture medium.
3. The reagent for rapidly detecting helicobacter pylori according to claim 1, wherein the ratio of the niclosite, the plant ash and the salt in the absorbent is 4.5:2.5:3, and the ratio of the vinegar, the disodium hydrogen phosphate and the sodium hydroxide in the additive is 6:2.5: 1.5.
4. The reagent for rapid detection of helicobacter pylori according to claim 1, wherein the ethanol solution is used for dissolving sodium chloride, urea, an indicator, an absorbent and an additive.
5. The reagent for rapid detection of helicobacter pylori according to claim 1, wherein the absorbent is prepared by a method comprising: firstly, respectively grinding the bischofite and the salt, grinding the bischofite and the salt into powder of 50 meshes to obtain bischofite powder and salt powder, adding the bischofite powder into a reaction kettle, adding hard water with high salinity, controlling the rotating speed of the reaction kettle at 420 r/min-550 r/min, raising the temperature to 55-70 ℃, then stirring and reacting for 15-25 minutes, then adding the salt powder into the reaction kettle, raising the temperature to 75-80 ℃, putting the plant ash into a refrigerating chamber, controlling the refrigerating temperature to-45-15 ℃, controlling the refrigerating time to 1-3 days, and adding the cooled plant ash into a reaction kettle, increasing the rotating speed of the reaction kettle to 720 r/min-920 r/min, and stirring for reacting for 4-12 hours to obtain the absorbent.
6. The reagent for rapidly detecting helicobacter pylori according to claim 1, wherein the preparation method of the additive comprises the following steps: firstly, cooling vinegar to-25 to-15 ℃, then adding disodium hydrogen phosphate and sodium hydroxide into a reaction kettle, controlling the rotating speed of the reaction kettle to be 220r/min to 350r/min, raising the temperature to 55 to 65 ℃, stirring for reaction for 15 to 25 minutes, then filling inert gas into the reaction kettle, then pouring the vinegar into the reaction kettle, continuously stirring for reaction for 15 to 25 minutes to obtain a mixed solution, pouring the mixed solution into drying and dehydrating equipment for evaporation until two thirds of the mixed solution is left, and thus obtaining the additive.
7. The reagent for rapidly detecting helicobacter pylori according to claim 1, wherein the culture medium is one or more of BME culture medium, RPMI-1640 culture medium, DMEM culture medium, F10 culture medium, Fischer's culture medium and MEM culture medium.
8. The reagent for rapid detection of helicobacter pylori according to claim 1, wherein the indicator is a pH indicator, and the sodium chloride and the urea are ground into powder.
9. The reagent for rapid detection of helicobacter pylori according to claim 1, wherein the absorbent and the additive are both decolorized by a decolorizing agent to be colorless.
10. The rapid detection reagent for helicobacter pylori according to claim 1, wherein the detection method of the detection reagent comprises: adding sodium chloride into an ethanol solution, stirring uniformly, adding urea and an indicator, continuing stirring, adding an absorbent and an additive, and stirring uniformly to obtain a test solution; dividing the culture medium into a bottom culture medium and a negative culture medium, respectively pouring the culture medium and the negative culture medium into two penicillin bottles, then equally dividing the prepared test solution and the test sample into two parts, respectively adding the two parts into the two penicillin bottles, observing the color change after stirring, and indicating that the test solution is positive if the color is yellow or purple, thereby indicating that the test solution contains the helicobacter pylori.
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| CN103265633A (en) * | 2013-05-06 | 2013-08-28 | 上海交通大学医学院附属第九人民医院 | Saliva streptococcus urase antibody, and preparation method and applications thereof |
| CN104458725A (en) * | 2014-12-08 | 2015-03-25 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN105755105A (en) * | 2016-04-19 | 2016-07-13 | 夏少时 | Reagent and kit for detecting helicobacter pylori and preparation method of kit |
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| CN111289505A (en) * | 2020-03-16 | 2020-06-16 | 南京康容健康科技有限公司 | Helicobacter pylori detect reagent box |
| CN112683893A (en) * | 2020-12-21 | 2021-04-20 | 南京康容健康科技有限公司 | High-sensitivity helicobacter pylori detection kit |
| CN113092459A (en) * | 2021-04-09 | 2021-07-09 | 许奕鹏 | Gel reagent and kit for fast screening of urease activity and fast screening method |
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2021
- 2021-12-28 CN CN202111623987.9A patent/CN114277086A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103265633A (en) * | 2013-05-06 | 2013-08-28 | 上海交通大学医学院附属第九人民医院 | Saliva streptococcus urase antibody, and preparation method and applications thereof |
| CN104458725A (en) * | 2014-12-08 | 2015-03-25 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN105755105A (en) * | 2016-04-19 | 2016-07-13 | 夏少时 | Reagent and kit for detecting helicobacter pylori and preparation method of kit |
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| CN113092459A (en) * | 2021-04-09 | 2021-07-09 | 许奕鹏 | Gel reagent and kit for fast screening of urease activity and fast screening method |
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