CN114270167A - 血液检测方法及血液分析*** - Google Patents

血液检测方法及血液分析*** Download PDF

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CN114270167A
CN114270167A CN201980099668.7A CN201980099668A CN114270167A CN 114270167 A CN114270167 A CN 114270167A CN 201980099668 A CN201980099668 A CN 201980099668A CN 114270167 A CN114270167 A CN 114270167A
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sample
blood
detected
optical information
intensity information
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易秋实
吴舒晨
汪东生
陈庚文
朱轲
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/1031Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/018Platelets
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1019Associating Coulter-counter and optical flow cytometer [OFC]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

一种血液检测方法和相应的血液分析***,该方法包括:用含有溶血剂的第一试剂处理血液样本以获得待测试样(S11),其中溶血剂将血液样本中的红细胞裂解为碎片;通过光学检测装置(50,200)获得待测试样的光学信息(S12),其中使待测试样中的粒子逐个通过光学检测装置(50,200)的检测区并且利用光学检测装置(50,200)的光源(101)对待测试样中的粒子进行照射,以获取待测试样的光学信息,其中光源(101)配置为发射波长小于488nm的光;和根据光学信息中的至少两种获得血小板的光学信息(S13)和/或网织红细胞的光学信息,其中在裂解血液样本中的红细胞的检测通道中获取血小板和/或网织红细胞的光学信息。

Description

PCT国内申请,说明书已公开。

Claims (36)

  1. PCT国内申请,权利要求书已公开。
CN201980099668.7A 2019-09-04 2019-09-04 血液检测方法及血液分析*** Pending CN114270167A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/104451 WO2021042307A1 (zh) 2019-09-04 2019-09-04 血液检测方法及血液分析***

Publications (1)

Publication Number Publication Date
CN114270167A true CN114270167A (zh) 2022-04-01

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Application Number Title Priority Date Filing Date
CN201980099668.7A Pending CN114270167A (zh) 2019-09-04 2019-09-04 血液检测方法及血液分析***

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CN (1) CN114270167A (zh)
WO (1) WO2021042307A1 (zh)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998022777A1 (en) * 1996-11-20 1998-05-28 Biochem Immunosystems Inc. Hemoglobin measurement device
SE531041C2 (sv) * 2006-07-17 2008-11-25 Hemocue Ab Räkning av trombocyter
CN102331411A (zh) * 2011-07-08 2012-01-25 无锡荣兴科技有限公司 一种具有蓝色半导体激光器的血液细胞分析仪
CN104458541A (zh) * 2013-09-12 2015-03-25 深圳迈瑞生物医疗电子股份有限公司 红细胞血红蛋白含量的分析方法、装置及血液细胞分析仪
JP6710512B2 (ja) * 2015-09-14 2020-06-17 シスメックス株式会社 血液分析装置、血液分析方法及びコンピュータプログラム
US10794814B2 (en) * 2017-05-25 2020-10-06 Abbott Laboratories Methods and systems for sample analysis

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