CN114260074B

CN114260074B - Frozen animal tissue homogenate equipment

Info

Publication number: CN114260074B
Application number: CN202111582903.1A
Authority: CN
Inventors: 牛宝泉
Original assignee: Shenzhen Huarui Model Organism Technology Co ltd
Current assignee: Shenzhen Huarui Model Organism Technology Co ltd
Priority date: 2021-12-22
Filing date: 2021-12-22
Publication date: 2023-06-13
Anticipated expiration: 2041-12-22
Also published as: CN114260074A

Abstract

The invention relates to the field of homogenizing equipment, in particular to frozen animal tissue homogenizing equipment, which comprises a base, wherein a nitrogen refrigerator is fixedly connected in the base, a cold chamber is also arranged in the base, the nitrogen refrigerator is communicated with the cold chamber through a cold air channel, a detachable stirring tank is arranged on the base, one side of the stirring tank is fixedly connected with a temperature controller for controlling the temperature in the cold chamber, a pressure seat is fixedly connected on a top plate above the base, a pressure rod groove is arranged in the pressure seat, a pressure rod electrically slides in the pressure rod groove, the lower end of the pressure rod is fixedly connected with a working plate, a motor protection shell is fixedly connected in the motor protection shell, the output end of the motor is fixedly connected with a driving shaft, frozen tissues can be efficiently crushed, and simultaneously, the homogenizing degree of three modes of scraping, cutting and grinding the frozen animal tissues is improved, and meanwhile, bubbles generated by homogenizing are removed, and the homogenizing quality is improved.

Description

Frozen animal tissue homogenate equipment

Technical Field

The invention relates to the field of homogenizing equipment, in particular to frozen animal tissue homogenizing equipment.

Background

The homogenizer is a machine for scattering and grinding animal and plant tissues into uniform paste, is widely used for homogenizing animal tissues, biological samples, foods, medicines, cosmetics, agricultural products, solid, semisolid and water-insoluble samples, and can be homogenized by 20ml to 250ml. The method is particularly suitable for preparing microorganism detection samples, the process of extracting bacteria from the samples is very simple and rapid, the samples and diluent (or emulsifying agent) are only required to be added into sterilized cups, then the cups are pressed onto a homogenizer, the time is started, the animal tissues to be processed are required to be subjected to freeze thawing for a plurality of times before being homogenized, and the following three problems exist in the current treatment of frozen animal tissue homogenization:

1. the efficiency of freezing and thawing animal tissues for many times is low, and the current homogenizing equipment cannot homogenate the animal tissues in a frozen state;

2. the common homogenizer has single action in the homogenizing process, the frozen tissues cannot be crushed in multiple stages, and the efficiency and quality of the homogenizing are low;

3. bubbles are easy to generate in the homogenizing process and are difficult to remove, so that the follow-up experiment is affected.

Disclosure of Invention

The invention aims at solving the problems in the prior art and provides a frozen animal tissue homogenizing device which can efficiently crush frozen tissues, simultaneously homogenize the frozen animal tissues in three modes of scraping, cutting and grinding, improve the degree of homogenization and remove bubbles generated by homogenization.

In order to achieve the above purpose, the present invention adopts the following technical scheme: the utility model provides a frozen animal tissue homogenate equipment, includes the base, be equipped with fixed connection's nitrogen gas refrigerator in the base, still seted up cold room in the base, connect through the air conditioning passageway between nitrogen gas refrigerator and the cold room, the base is in be equipped with detachable agitator tank on the cold room, the cold room is in temperature controller of agitator tank one side fixed connection control temperature, the base top is equipped with the pressure seat of fixed connection on the roof, set up the pressure pole groove in the pressure seat, the pressure pole is in the electric slip of pressure pole inslot, pressure pole lower extreme fixed connection working plate, be equipped with fixed connection's motor protective housing on the working plate, be equipped with fixed connection's motor in the motor protective housing, motor output fixed connection driving shaft.

Preferably, the rotatable stopper of fixed connection is equipped with on the driving shaft, be equipped with on the driving shaft rotatable connection's between the rotatable stopper mount, sliding connection slidable stopper about the mount both sides, set up the restriction on the agitator tank slidable stopper pivoted, the mount lower extreme is equipped with the tooth face board, the driving shaft is in the first gear of tooth face board section fixed connection, the driving shaft is in first gear below sliding connection rotates the exhaust seat, the symmetry is equipped with the branch of rotating connection on the rotation exhaust seat, branch upper end fixed connection with first gear engagement's second gear, branch still with tooth face board meshing, two branch are in the slant setting of second gear below fixed connection symmetry can produce the suction fan leaf of suction to bottom air when rotating together.

Preferably, a fixed spring block fixedly connected to the driving shaft is arranged above the rotating exhaust seat, a first spring is arranged between the fixed spring block and the rotating exhaust seat, a plurality of scrapers arranged in a circular array are fixedly connected to the lower end of the driving shaft, scraping grooves for scraping ice are formed in a staggered mode in the downward direction of the scrapers, and a rotating cutter for stirring ice scraps scraped by the scrapers is fixedly connected to the lower end of the supporting rod.

Preferably, the lower end of the driving shaft is provided with a grinding cavity, a fixed rod fixedly connected with the grinding cavity is arranged in the grinding cavity, and the upper end of the fixed rod is provided with a grinding blade fixedly connected with the grinding blade capable of further grinding the crushed ice dust below the rotary exhaust seat.

Preferably, two foam collecting ports for collecting air bubbles are symmetrically formed at the bottom edge of the rotary exhaust seat, a curved foam inlet channel taking the foam collecting ports as inlets is formed in the rotary exhaust seat, the rear section of the foam inlet channel is a gradually reduced extrusion channel for crushing air bubbles, the rear section of the extrusion channel is a fine separation channel, the separation channel is divided into a separation channel with the upper end for exhausting upwards and a extrusion channel with the lower end for exhausting slurry, the tail end of the extrusion channel is communicated with the outside of the lower end face of the rotary exhaust seat, and the tail end of the separation channel is communicated with the outside of the upper end face of the rotary exhaust seat.

Preferably, the rotary exhaust seat is symmetrically provided with bubble pressing grooves at the extrusion channel, bubble pressing blocks for extruding bubbles are slidably connected in the bubble pressing grooves, a plurality of groups of second springs are arranged between the bubble pressing blocks and the bubble pressing grooves, and bubble breaking needles which are fixedly connected and are arranged on opposite surfaces of the two bubble pressing blocks in a staggered manner and used for breaking bubbles are arranged;

the beneficial effects are that: the invention scrapes the frozen surface by the scraper and cuts the frozen animal tissue;

scraping frozen tissues, and homogenizing the frozen animal tissues in a cutting mode and a grinding mode, so that the degree of homogenization is improved;

meanwhile, the arrangement of the rotary exhaust seat enables bubbles generated by homogenization to be concentrated by centrifugal force, and the bubbles are removed through the extrusion channel, so that the quality of the homogenization is improved.

Drawings

FIG. 1 is a schematic view of the present invention;

FIG. 2 is a schematic diagram of a structural implementation of the present invention;

FIG. 3 is an enlarged schematic view of FIG. 2 at A;

FIG. 4 is an enlarged schematic view of FIG. 2B;

FIG. 5 is a schematic view of the direction C-C in FIG. 3;

FIG. 6 is a schematic view of the direction D-D in FIG. 4;

FIG. 7 is a schematic view of the E-E direction of FIG. 6;

fig. 8 is an enlarged schematic view of fig. 6 at F.

In the drawing, a base 10, a nitrogen refrigerator 11, a cold room 12, a cold air channel 13, a stirring tank 14, a temperature controller 15, a pressure seat 16, a pressure rod 17, a pressure rod groove 18, a working plate 19, a motor protection shell 20, a motor 21, a driving shaft 22, a limit groove 23, a fixing frame 24, a slidable limit block 25, a rotatable limit block 26, a supporting rod 27, a second gear 28, a first gear 29, a suction fan blade 30, a tooth surface plate 31, a fixed spring block 32, a first spring 33, a rotary exhaust seat 34, a foam collecting port 35, a foam inlet channel 36, a rotary cutter 37, a scraper 38, a scraping groove 39, a grinding cavity 40, a fixed rod 41, a crushing blade 42, a separation channel 43, a separation channel 44, a separation liquid channel 45, a squeezing channel 46, a foam pressing groove 47, a foam pressing block 48, a second spring 49 and a foam breaking needle 50 are shown.

Detailed Description

The following are specific embodiments of the present invention and the technical solutions of the present invention will be further described with reference to the accompanying drawings, but the present invention is not limited to these embodiments.

In the description of the present invention, it should be noted that, the azimuth or positional relationship indicated by the terms "inner", "lower", etc. are based on the azimuth or positional relationship shown in the drawings, or the azimuth or positional relationship that the inventive product is conventionally put in use, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific azimuth, be configured and operated in a specific azimuth, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

Referring to fig. 1-8, a frozen animal tissue homogenizing apparatus comprises a base 10, wherein a nitrogen refrigerator 11 is fixedly connected in the base 10, a cold chamber 12 is further arranged in the base 10, the nitrogen refrigerator 11 is communicated with the cold chamber 12 through a cold air channel 13, a detachable stirring tank 14 is arranged on the cold chamber 12, the cold chamber 12 is fixedly connected with a temperature controller 15 for controlling temperature on one side of the stirring tank 14, a pressure seat 16 is arranged above the base 10 and fixedly connected with a top plate, a pressure rod groove 18 is arranged in the pressure seat 16, a pressure rod 17 electrically slides in the pressure rod groove 18, the lower end of the pressure rod 17 is fixedly connected with a working plate 19, a motor protection shell 20 is arranged on the working plate 19, a motor 21 is fixedly connected in the motor protection shell 20, and the output end of the motor 21 is fixedly connected with a driving shaft 22.

Further, a rotatable limiting block 26 fixedly connected is arranged on the driving shaft 22, a fixed frame 24 rotationally connected between the rotatable limiting blocks 26 is arranged on the driving shaft 22, the two sides of the fixed frame 24 are vertically and slidably connected with the slidable limiting blocks 25, a 51 for limiting the rotation of the slidable limiting blocks 25 is arranged on the stirring tank 14, a tooth panel 31 is arranged at the lower end of the fixed frame 24, the driving shaft 22 is fixedly connected with a first gear 29 at the tooth surface 31 section, the driving shaft 22 is slidingly connected with a rotary exhaust seat 34 below the first gear 29, rotationally connected supporting rods 27 are symmetrically arranged on the rotary exhaust seat 34, the upper ends of the supporting rods 27 are fixedly connected with a second gear 28 meshed with the first gear 29, the supporting rods 27 are meshed with the tooth surface 31, and two supporting rods 27 are fixedly connected with symmetrically arranged oblique air suction fan blades 30 capable of generating suction force to bottom air when rotating together below the second gear 28.

Further, a fixed spring block 32 fixedly connected to the driving shaft 22 is arranged above the rotating exhaust seat 34, a first spring 33 is arranged between the fixed spring block 32 and the rotating exhaust seat 34, the lower end of the driving shaft 22 is fixedly connected with a plurality of scrapers 38 arranged in a circular array, the scrapers 38 are provided with scraping grooves 39 which are staggered and used for scraping ice, and the lower end of the supporting rod 27 is fixedly connected with a rotating cutter 37 for stirring ice scraps scraped by the scrapers 38.

Further, a grinding cavity 40 is formed at the lower end of the driving shaft 22, a fixed rod 41 is fixedly connected in the grinding cavity 40, and a grinding blade 42 which is fixedly connected and can grind the crushed ice dust below the rotary exhaust seat 34 is arranged at the upper end of the fixed rod 41.

Further, two foam collecting ports 35 for collecting air bubbles are symmetrically arranged at the bottom edge of the rotary exhaust seat 34, a curved foam inlet channel 36 taking the foam collecting ports 35 as inlets is arranged in the rotary exhaust seat 34, the rear section of the foam inlet channel 36 is a gradually reduced extrusion channel 46 for crushing air bubbles, the rear section of the extrusion channel 46 is a fine separation channel 43, the separation channel 43 is divided into a separation channel 44 with the upper end for exhausting air upwards and an extrusion channel 46 with the lower end for exhausting slurry, the tail end of the extrusion channel 46 is communicated with the outside of the lower end face of the rotary exhaust seat 34, and the tail end of the separation channel 44 is communicated with the outside of the upper end face of the rotary exhaust seat 34.

Further, the rotary exhaust seat 34 is symmetrically provided with bubble pressing grooves 47 at the extrusion channel 46, bubble pressing blocks 48 which are slidably connected with extrusion bubbles are arranged between the bubble pressing blocks 48 and the bubble pressing grooves 47, a plurality of groups of second springs 49 are arranged between the bubble pressing blocks 48 and the bubble pressing grooves 47, and bubble breaking needles 50 which are fixedly connected and are arranged in a staggered manner and used for breaking bubbles are arranged on opposite surfaces of the two bubble pressing blocks 48.

Working principle: in the initial state, the pressure rod 17 is contracted in the pressure rod groove 18, the driving shaft 22 does not enter the stirring tank 14, the nitrogen refrigerator 11 is started, and nitrogen enters the cold chamber 12 through the cold air channel 13 for cooling;

the method comprises the steps that an animal tissue is put into a stirring tank 14 by a worker to freeze, a nitrogen refrigerator 11 is closed after freezing, meanwhile, the temperature in a refrigerating chamber 12 is controlled to be always between 0 ℃ and 4 ℃ through a temperature controller 15, homogenate buffer is added into the stirring tank 14, a pressure seat 16 is controlled to extend out of a pressure rod groove 18, equipment at the lower end is driven to enter the stirring tank 14 until a scraper 38 touches the ice surface, a motor 21 is turned on, the motor 21 drives a fixedly connected driving shaft 22 to rotate, the driving shaft 22 drives a scraper 38 at the bottom to rotate, meanwhile, a pressure rod 17 keeps downward pressure, the scraper 38 has enough pressure on the ice surface to scrape the ice surface, more ice fragments scraped each time are pushed to a rotary cutter 37, the first gear 29 drives a meshed second gear 28 to rotate due to the rotation of the driving shaft 22, a slidable limiting block 25 which is in sliding connection on a fixing frame 24 is limited on the stirring tank 14 and cannot rotate, the fixing frame 24 cannot rotate, a tooth surface plate 31 below the 2 is meshed with the second gear 28, the second gear 28 is meshed with the second gear 28, and the second gear 28 is meshed with the second gear 28 and rotates around the second gear 28 in a rotating manner, and is fixedly connected with the scraper bar 37, and the second gear is meshed with the second gear 28 and rotates around the second gear 28, so that the scraper is fixedly connected with the scraper bar and rotates, and is connected with the scraper bar carrier bar and the scraper bar is rotated, and is fixed, so that is connected with the scraper bar is rotated, and is fixed, and has a supporting rod is fixed, and a supporting rod is formed; because more and more substances are scraped by the scraper 38, the rotating exhaust seat 34 which is slidably connected to the driving shaft 22 is pushed to move upwards against the elasticity of the first spring 33, the existence of the rotating exhaust seat 34 can reduce the air entering and reduce the generation of bubbles until frozen tissues are completely ground, substances in the stirring tank 14 are easy to be in a state that frozen substances and liquid exist simultaneously due to the existence of buffer solution and the control of low temperature, at the moment, the rotating exhaust seat 34 is pressed on the liquid surface, the fixing rod 41 is fixedly connected in the driving shaft 22, the driving shaft 22 drives the crushing blade 42 to rotate when rotating, the crushing blade 42 sucks tissues in the upper end homogenate into the grinding cavity 40, and the tissues are further refined through the combined action of further crushing of the crushing blade 42 and the grinding cavity 40 of the fixing rod 41, and simultaneously come out from the bottom of the driving shaft 22 to perform continuous circulation;

due to the arrangement of the foam collecting opening 35 at the bottom of the rotary exhaust seat 34, air bubbles generated during stirring are accumulated at the foam collecting opening 35 under the action of centrifugal force and buoyancy, enter the foam inlet channel 36 along with the rotation of the rotary exhaust seat 34, the air bubbles enter the foam inlet channel 36 and then are smaller through the channel at the extruding channel 46, larger air bubbles are crushed due to the fact that the channel at the extruding channel 46 is smaller, meanwhile, if more stubborn air bubbles and tiny air bubbles are formed, the bubbles can be broken through the breaking needle 50, further air passes through the separation air channel 44 to the upper side of the rotary exhaust seat 34, liquid forming the air bubbles reenters the lower side of the rotary exhaust seat 34 through the separation liquid channel 45, the suction fan blade 30 fixedly connected with the support rod 27 rotates around the driving shaft 22, suction force is generated for air below, air pressure below is reduced, and the air bubbles are further discharged through the separation air channel 44, so that the existence of air bubbles in homogenate is avoided.

The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent structures or equivalent processes using the descriptions and the drawings of the present invention or directly or indirectly applied to other related technical fields are included in the scope of the invention.

Claims

1. The utility model provides a frozen animal tissue homogenate equipment, includes base (10), its characterized in that, be equipped with fixed connection's nitrogen gas refrigerator (11) in base (10), cold room (12) have still been seted up in base (10), put through cold air flue (13) between nitrogen gas refrigerator (11) and cold room (12), base (10) are in be equipped with detachable agitator tank (14) on cold room (12), cold room (12) are in temperature controller (15) of agitator tank (14) one side fixed connection control temperature, base (10) top is equipped with fixed connection pressure seat (16) on the roof, pressure bar groove (18) have been seted up in pressure seat (16), pressure bar (17) are in electric slip in pressure bar groove (18), pressure bar (17) lower extreme fixed connection working plate (19), be equipped with fixed connection's motor protective housing (20) on working plate (19), be equipped with fixed connection's motor (21) in protective housing, motor (21) output end fixed connection (22). The device comprises a driving shaft (22), a rotatable limiting block (26) fixedly connected with the driving shaft (22), a fixed frame (24) rotationally connected between the rotatable limiting blocks (26) is arranged on the driving shaft (22), two sides of the fixed frame (24) are vertically and slidably connected with a slidable limiting block (25), a stirring tank (14) is provided with a gear face plate (31) for limiting the rotation (51) of the slidable limiting block (25), a first gear (29) is fixedly connected with the driving shaft (22) at a gear face plate (31) section, a rotary exhaust seat (34) is slidingly connected below the first gear (29) by the driving shaft (22), rotationally connected supporting rods (27) are symmetrically arranged on the rotary exhaust seat (34), a second gear (28) meshed with the first gear (29) is fixedly connected with the upper ends of the supporting rods (27), the supporting rods (27) are also meshed with the gear face plate (31), and two supporting rods (27) are fixedly connected with the bottom of a pair of obliquely-arranged fan blades (30) which can suck air when the blades are rotationally symmetrically arranged below the second gear (28); a fixed spring block (32) fixedly connected to the driving shaft (22) is arranged above the rotating exhaust seat (34), a first spring (33) is arranged between the fixed spring block (32) and the rotating exhaust seat (34), the lower end of the driving shaft (22) is fixedly connected with a plurality of scrapers (38) arranged in a circular array, the scrapers (38) are downwards provided with scraping grooves (39) which are staggered and used for scraping ice, and the lower end of the supporting rod (27) is fixedly connected with a rotating cutter (37) for stirring ice scraps scraped by the scrapers (38); the lower end of the driving shaft (22) is provided with a grinding cavity (40), a fixed rod (41) which is fixedly connected is arranged in the grinding cavity (40), and a grinding blade (42) which is fixedly connected and can further grind the crushed ice dust below the rotary exhaust seat (34) is arranged at the upper end of the fixed rod (41); two foam collecting ports (35) for collecting air bubbles are symmetrically formed in the bottom edge of the rotary exhaust seat (34), a curved foam inlet channel (36) taking the foam collecting ports (35) as inlets is formed in the rotary exhaust seat (34), the rear section of the foam inlet channel (36) is a gradually reduced extrusion channel (46) for crushing the air bubbles, the rear section of the extrusion channel (46) is a fine separation channel (43), the separation channel (43) is divided into a separation channel (44) with the upper end for exhausting upwards and an extrusion channel (46) with the lower end for exhausting slurry, the tail end of the extrusion channel (46) is communicated with the outside of the lower end face of the rotary exhaust seat (34), and the tail end of the separation channel (44) is communicated with the outside of the upper end face of the rotary exhaust seat (34); the rotary exhaust seat (34) is characterized in that foam pressing grooves (47) are symmetrically formed in the extrusion channel (46), foam pressing blocks (48) for pressing air bubbles are connected in a sliding mode in the foam pressing grooves (47), a plurality of groups of second springs (49) are arranged between the foam pressing blocks (48) and the foam pressing grooves (47), and foam breaking needles (50) which are fixedly connected and are arranged in a staggered mode and used for breaking the air bubbles are arranged on opposite surfaces of the foam pressing blocks (48).