CN114246822A - Micro-ecological composition for repairing and anti-aging skin and preparation method and application thereof - Google Patents
Micro-ecological composition for repairing and anti-aging skin and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of cosmetics, and discloses a skin repairing and anti-aging microecological composition, and a preparation method and application thereof. The skin-repairing and anti-aging microecological composition comprises lactobacillus fermentation lysate, beta-glucan fermentation broth and polyquaternium-51. The composition utilizes the self structure characteristic of polyquaternium-51 to promote deep penetration of active ingredients, and can improve skin color and reduce wrinkle formation from skin; meanwhile, the lactobacillus fermentation lysate in the composition is subjected to wall breaking treatment by adopting a high-pressure homogenization method, so that the active ingredients are retained, and simultaneously, the sensitization ingredients in cytoplasm are removed.
Description
Technical Field
The invention relates to the field of cosmetics, in particular to a skin repairing and anti-aging microecological composition, and a preparation method and application thereof.
Background
The skin barrier is an important organ to protect against external environmental stimuli, and in most consumer cognizances, the skin barrier is generally referred to as a physical barrier. In practice, however, the skin barrier is largely divided into four layers, from the outside to the inside, a microbial barrier, a chemical barrier, a physical barrier and an immunological barrier. The microbial barrier is the outermost layer of the skin barrier, is composed of microbial communities on the surface layer of the skin, maintains the microbial ecological balance of the skin, and participates in various physiological functions of the skin and the like. The chemical barrier comprises factors that maintain the acidic pH of the skin surface and Natural Moisturizing Factors (NMF). The physical barrier is a 'brick wall' structure formed by a sebum membrane, keratinocytes and intercellular lipids, and mainly plays a role in keeping skin moisture, preventing water in vivo from evaporating and resisting external foreign matters from invading the body. The immune barrier is the last part of the skin barrier, with various immune cells in the epidermis and dermis that further promote barrier repair, highly correlated with other skin barriers.
The repair of damaged skin barriers in cosmetics is often spread around brick wall structures, often ignoring the importance, relevance of other barriers. In recent years, as Chinese consumers know probiotics more and more deeply, the concept of skin care is more and more mature, and the micro-ecosystem imbalance is realized, so that the quantity of the probiotics is reduced and even disappears, and the flora imbalance is further caused; the skin resistance is reduced, and the skin is easy to be inflamed and infected; skin is abnormally sensitive and repeatedly grows pox; skin barrier function is reduced, and skin problems such as dryness, tightness, itching and the like easily occur. There are two main types of skin care products aimed at micro ecology at present: one is to directly utilize the metabolite of the probiotics, and the other is to add prebiotics (ingredients which have the effect of promoting the growth of the probiotics) as components into the product, thereby promoting the growth of the probiotics on the skin.
Lactic acid bacteria are indispensable flora for human body, are widely applied to food industry, and are also concerned by people with the understanding of importance and relevance of other skin barriers besides brick wall structures in cosmetics. Lactic acid bacteria can undergo slow fermentation in an anaerobic environment for several weeks, converting carbohydrates and sugars into enzymes and amino acids preferred by the skin, and decomposing and micronizing the active ingredients. However, the skin is used as a human body barrier, and the effect of information transmission of probiotics through the skin barrier is very little, so that the probiotics is directly coated with lactic acid bacteria or metabolins, and the antagonism of the skin is easily induced, thereby causing the skin irritation problem.
Disclosure of Invention
The invention aims to overcome the defects of the background technology and provides a skin repairing and anti-aging microecological composition, a preparation method and application thereof. The composition of the invention utilizes the self structural characteristics of polyquaternium-51 to promote the deep penetration of active ingredients, and can improve the complexion from the interior of the skin and reduce the generation of wrinkles; meanwhile, the lactobacillus fermentation lysate in the composition is subjected to wall breaking treatment by adopting a high-pressure homogenization method, so that the active ingredients are retained, and simultaneously, the sensitization ingredients in cytoplasm are removed.
To achieve the object of the present invention, the skin-repairing, anti-aging microecological composition of the present invention comprises a lactobacillus fermentation lysate, a β -glucan fermentation broth, and polyquaternium-51.
Further, in some embodiments of the invention, the lactobacillus fermentation lysate is a lactobacillus fermentation lysate that has been depleted of cell wall particles and cytoplasmic protein components.
Further, in some embodiments of the invention, the method of producing a lactobacillus fermentation lysate comprises: inoculating lactobacillus in MRS liquid culture medium in an inoculum size of 0.5-5%, culturing in a shaking table at 35-45 deg.C and 200rpm for 1-3 days, pasteurizing, centrifuging, collecting lactobacillus thallus, washing with sterilized distilled water, and suspending the thallus in sterilized distilled water; taking suspended bacteria liquid, performing wall breaking treatment by using a high-pressure homogenizer at the temperature of 70-90 ℃ and the pressure of 400-600bar, breaking the wall, centrifuging, collecting supernatant, standing for 12-24h at the temperature of 4-6 ℃, filtering, and collecting filtrate to obtain the lactobacillus fermentation lysate.
Further, in some embodiments of the invention, the beta-glucan fermentation broth is prepared by enzymatic hydrolysis of polysaccharide molecular weight using a beta-1, 3-glucanase endogenous to the fermentation system at a later stage of fermentation.
Further, in some embodiments of the present invention, the method for preparing the beta-glucan fermentation broth comprises:
a. culturing Schizophyllum commune strains for 6-8 days at 25-30 ℃, inoculating mycelia from an inclined test tube, transferring into a seed bottle with a liquid loading capacity of 100-;
b. transferring the second-stage seed culture solution into a fermentation tank with an inoculum size of 2-5%, adjusting the internal pressure of the tank to be 0.02-0.04MPa, and controlling the ventilation quantity according to the volume of the material liquid: the volume of air is 1: 0.5-1.2, stirring speed of 100-200r/min, temperature of 25-30 ℃, culturing for 3.5-4.5 days, centrifuging to remove thalli, performing polysaccharide molecular weight enzymolysis by using endogenous beta-1, 3-glucanase of a fermentation system, temperature of 37-43 ℃, pH of 5.3-5.8, enzymolysis time of 18-30h, filtering, and collecting filtrate to obtain beta-glucan fermentation liquor.
Further, in some embodiments of the invention, the polysaccharide content of the beta-glucan fermentation broth is 0.4-1.2%.
Further, in some embodiments of the present invention, the polyquaternium-51 is a 2-6% by mass aqueous polyquaternium-51 solution.
Further, in some embodiments of the invention, the lactobacillus fermentation lysate: beta-glucan fermentation broth: the mass ratio of polyquaternium-51 is 1-5: 0.5-2: 1-3.
Preferably, in some embodiments of the invention, the lactobacillus fermentation lysate: beta-glucan fermentation broth: the mass ratio of polyquaternium-51 is 2.5-3.5: 0.8-2: 1-3.
In another aspect, the present invention also provides a method for preparing the aforementioned skin-repairing anti-aging microecological composition, comprising the steps of:
(1) culturing Schizophyllum commune strains for 6-8 days at 25-30 ℃, inoculating mycelia from an inclined test tube, transferring into a seed bottle with a liquid loading capacity of 100-;
(2) transferring the second-stage seed culture solution into a fermentation tank with an inoculum size of 2-5%, adjusting the internal pressure of the tank to be 0.02-0.04MPa, and controlling the ventilation quantity according to the volume of the material liquid: the volume of air is 1: 0.5-1.2, stirring at a speed of 100-200r/min, culturing at 25-30 ℃ for 3.5-4.5 days, centrifuging to remove thallus, performing polysaccharide molecular weight enzymolysis by using endogenous beta-1, 3-glucanase of a fermentation system at a temperature of 37-43 ℃, a pH value of 5.3-5.8 and an enzymolysis time of 18-30h, filtering, and collecting filtrate to obtain beta-glucan fermentation liquor for later use;
(3) inoculating lactobacillus in MRS liquid culture medium in an inoculum size of 0.5-5%, culturing in a shaking table at 35-45 deg.C and 200rpm for 1-3 days, pasteurizing, centrifuging, collecting lactobacillus thallus, washing with sterilized distilled water, and suspending the thallus in sterilized distilled water;
(4) taking suspended bacteria liquid, performing wall breaking treatment by using a high-pressure homogenizer at the temperature of 70-90 ℃ and the pressure of 400-600bar, breaking the wall, centrifuging, collecting supernatant, standing for 12-24h at the temperature of 4-6 ℃, filtering, and collecting filtrate to obtain a lactobacillus fermentation lysate for later use;
(5) uniformly mixing the lactobacillus fermentation lysate, the beta-glucan fermentation liquor and the polyquaternium-51 aqueous solution according to the proportion to obtain the skin repairing and anti-aging microecological composition.
Further, in some embodiments of the present invention, the culture medium for culturing the strain in step (1) is composed of glucose25-35g/L, 2-4g/L yeast extract, KH2PO4 0.8-1.2g/L，MgSO4·7H2O0.4-0.6 g/L, pH 4-5, adding 1.5-2.5% agar.
Further, in some embodiments of the present invention, the composition of the fermentation tank medium in step (2) is 25-35g/L glucose, 2-4g/L yeast extract and KH2PO4 0.8-1.2g/L，MgSO4·7H20.4-0.6g/L of O, 0.04-0.06% of defoaming agent and 4-5 of pH.
Further, in some embodiments of the invention, the wall breaking is performed by wall breaking for 4-6min, and repeating for 3-5 times with a gap of 4-6 min.
In still another aspect, the invention also provides an application of the skin repairing and anti-aging microecological composition, wherein the composition is prepared into cosmetics or used as a cosmetic auxiliary agent and additive.
Further, in some embodiments of the present invention, the cosmetic includes, but is not limited to, lotions, essences, creams, and the like.
Compared with the prior art, the invention has the following advantages:
(1) the lactobacillus fermentation lysate in the composition is subjected to wall breaking treatment of lactobacillus by using a high-pressure homogenization method, releases intracellular active substances, and removes macromolecular protein components in cytoplasm through cooling and filtering, so that the influence of allergic components when lactobacillus acts on skin is minimized;
(2) in the later fermentation stage of the beta-glucan fermentation liquor in the composition, the product polysaccharide is degraded by endogenous beta-1, 3-glucan endonuclease, so that fermentation and degradation are integrated, the preparation process is simplified, and the production cost is reduced;
(3) the polyquaternium-51 in the composition disclosed by the invention encapsulates the active substances obtained after the wall breaking of the lactobacillus and the micromolecular polysaccharide obtained after the degradation of the beta-glucan by utilizing the cell membrane-imitated structure characteristic of the nano micelle, so that the permeability of the active substances is effectively improved, and the active substances act on the dermis.
Drawings
FIG. 1 is a schematic diagram showing the results of the test for keratinocyte gene expression according to the present invention;
FIG. 2 is the result of the skin gloss improvement test according to the present invention;
fig. 3 is a result of an experiment of the reduction rate of the total size of the canthus wrinkles according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention. It is to be understood that the following description is only illustrative of the present invention and is not to be construed as limiting the present invention.
The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
Furthermore, the description below of the terms "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like, means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily for the same embodiment or example. Further, the technical features of the embodiments of the present invention may be combined with each other as long as they do not conflict with each other.
Examples 1 to 7
A skin-repairing and anti-aging microecological composition is prepared by adopting the following processes:
s1, taking a schizophyllum commune strain for culture, culturing an inclined plane seed at 25 ℃ for 7 days, taking two-ring hyphae from an inclined plane test tube by using an inoculating loop, transferring the two-ring hyphae into a 250ml primary seed bottle with the liquid loading capacity of 120ml, culturing at 25 ℃ at the rotating speed of 180r/min for 3 days, transferring the primary seed culture solution into a 250ml secondary seed culture bottle with the inoculation amount of 5%, and continuously culturing for 7 days to obtain a secondary seed culture solution;
s2, transferring the secondary seed culture solution into a fermentation tank with 3% inoculation amount, adjusting the pressure in the fermentation tank to be 0.04MPa, keeping the ventilation rate at 1:1 (volume of material liquid: volume of air), stirring at a rotation speed of 180r/min and a temperature of 25 ℃, culturing for 4 days, centrifuging at a high speed in a tubular manner to remove thalli, performing polysaccharide molecular weight enzymolysis by utilizing endogenous beta-1, 3-glucanase of a fermentation system at a temperature of 40 ℃, a pH value of 5.5 and an enzymolysis time of 24 hours, filtering, and collecting filtrate to obtain beta-glucan fermentation liquor for later use;
s3, inoculating lactobacillus to MRS liquid culture medium according to the inoculation amount of 2%, culturing for 3 days at 37 ℃ and 180rpm in a shaking table, pasteurizing for 30min, centrifuging (5000rpm, 15min), collecting lactobacillus thallus, washing with sterilized distilled water for 3 times, and suspending the thallus in 5 times of sterilized distilled water for later use;
s4, taking the suspended bacteria liquid, breaking the walls by using a high-pressure homogenizer at 80 ℃, 500bar, 20min (5 min for wall breaking, 5min for gap, repeated 4 times), centrifuging, collecting the supernatant, standing for 24h at 4 ℃, filtering, and collecting the filtrate to obtain the lactobacillus fermentation lysate for later use;
s5, uniformly mixing the lactobacillus fermentation lysate, the beta-glucan fermentation liquor and the polyquaternium-51 aqueous solution according to the proportion to obtain the skin repairing and anti-aging microecological composition.
Comparative example 1
A skin-repairing and anti-aging microecological composition is prepared by adopting the following processes:
s1, culturing Schizophyllum commune strains, culturing the slant seeds at 25 ℃ for 7 days, taking two-ring hyphae from a slant test tube by using an inoculating loop, transferring the two-ring hyphae into a 250ml first-stage seed bottle with the liquid loading capacity of 120ml, culturing at 25 ℃ and at the rotating speed of 180r/min, transferring the first-stage seed culture solution into a 250ml second-stage seed culture bottle with the inoculation amount of 5 percent after culturing for 3 days, continuously culturing for 7 days, centrifuging at a high speed in a tubular mode to remove thalli, and collecting filtrate to obtain beta-glucan fermentation liquor for later use;
s2, inoculating lactobacillus to MRS liquid culture medium according to the inoculation amount of 2%, culturing for 3 days at 37 ℃ and 180rpm in a shaking table, pasteurizing for 30min, centrifuging (5000rpm, 15min), collecting lactobacillus thallus, washing with sterilized distilled water for 3 times, and suspending the thallus in 5 times of sterilized distilled water for later use;
s3, taking the suspended bacteria liquid, breaking the walls by using a high-pressure homogenizer at 80 ℃, 500bar, 20min (5 min for wall breaking, 5min for gap, repeated 4 times), centrifuging, collecting the supernatant, standing for 24h at 4 ℃, filtering, and collecting the filtrate to obtain the lactobacillus fermentation lysate for later use;
s4, uniformly mixing the lactobacillus fermentation lysate, the beta-glucan fermentation liquor and the polyquaternium-51 aqueous solution according to the proportion to obtain the skin repairing and anti-aging microecological composition.
Comparative example 2
A skin-repairing and anti-aging microecological composition is prepared by adopting the following processes:
s1, taking a schizophyllum commune strain for culture, culturing an inclined plane seed at 25 ℃ for 7 days, taking two-ring hyphae from an inclined plane test tube by using an inoculating loop, transferring the two-ring hyphae into a 250ml primary seed bottle with the liquid loading capacity of 120ml, culturing at 25 ℃ at the rotating speed of 180r/min for 3 days, transferring the primary seed culture solution into a 250ml secondary seed culture bottle with the inoculation amount of 5%, and continuously culturing for 7 days to obtain a secondary seed culture solution;
s2, transferring the secondary seed culture solution into a fermentation tank with 3% inoculation amount, adjusting the pressure in the fermentation tank to be 0.04MPa, keeping the ventilation rate at 1:1 (volume of material liquid: volume of air), stirring at a rotation speed of 180r/min and a temperature of 25 ℃, culturing for 4 days, centrifuging at a high speed in a tubular manner to remove thalli, performing polysaccharide molecular weight enzymolysis by utilizing endogenous beta-1, 3-glucanase of a fermentation system at a temperature of 40 ℃, a pH value of 5.5 and an enzymolysis time of 24 hours, filtering, and collecting filtrate to obtain beta-glucan fermentation liquor for later use;
s3, inoculating lactobacillus to MRS liquid culture medium according to the inoculation amount of 2%, culturing for 3 days at 37 ℃ and 180rpm in a shaking table, pasteurizing for 30min, centrifuging (5000rpm, 15min), collecting lactobacillus thallus, washing with sterilized distilled water for 3 times, and suspending the thallus in 5 times of sterilized distilled water for later use;
s4, taking suspended bacterium liquid, performing wall breaking treatment by ultrasonic waves at 80 ℃, at normal pressure for 20min (wall breaking for 5min, and repeating for 4 times at intervals for 5min), centrifuging, collecting supernatant, standing for 24h at 4 ℃, filtering, and collecting filtrate to obtain lactobacillus fermentation lysate for later use;
s5, uniformly mixing the lactobacillus fermentation lysate, the beta-glucan fermentation liquor and the polyquaternium-51 aqueous solution according to the proportion to obtain the skin repairing and anti-aging microecological composition.
Comparative example 3
A skin-repairing and anti-aging microecological composition is prepared by adopting the following processes:
s1, taking a schizophyllum commune strain for culture, culturing an inclined plane seed at 25 ℃ for 7 days, taking two-ring hyphae from an inclined plane test tube by using an inoculating loop, transferring the two-ring hyphae into a 250ml primary seed bottle with the liquid loading capacity of 120ml, culturing at 25 ℃ at the rotating speed of 180r/min for 3 days, transferring the primary seed culture solution into a 250ml secondary seed culture bottle with the inoculation amount of 5%, and continuously culturing for 7 days to obtain a secondary seed culture solution;
s2, transferring the secondary seed culture solution into a fermentation tank with 3% inoculation amount, adjusting the pressure in the fermentation tank to be 0.04MPa, keeping the ventilation rate at 1:1 (volume of material liquid: volume of air), stirring at a rotation speed of 180r/min and a temperature of 25 ℃, culturing for 4 days, centrifuging at a high speed in a tubular manner to remove thalli, performing polysaccharide molecular weight enzymolysis by utilizing endogenous beta-1, 3-glucanase of a fermentation system at a temperature of 40 ℃, a pH value of 5.5 and an enzymolysis time of 24 hours, filtering, and collecting filtrate to obtain beta-glucan fermentation liquor for later use;
s3, inoculating lactobacillus to MRS liquid culture medium according to the inoculation amount of 2%, culturing for 3 days at 37 ℃ and 180rpm in a shaking table, pasteurizing for 30min, centrifuging (5000rpm, 15min), collecting lactobacillus thallus, washing with sterilized distilled water for 3 times, and suspending the thallus in 5 times of sterilized distilled water for later use;
s4, taking the suspended bacteria liquid, breaking the walls by using a high-pressure homogenizer at 80 ℃, 500bar, 20min (5 min for wall breaking, 5min for gap, repeated 4 times), centrifuging, collecting the supernatant, standing for 24h at 4 ℃, filtering, and collecting the filtrate to obtain the lactobacillus fermentation lysate for later use;
s5, mixing the lactobacillus fermentation lysate and the beta-glucan fermentation liquid uniformly according to the proportion to obtain the skin repairing and anti-aging microecological composition.
The compositions of examples 1 to 7 and comparative examples 1 to 3 were prepared by the above-described method, wherein the mass ratios of the respective components are shown in table 1, and the mass concentration of the aqueous solution of polyquaternium-51 was 4%.
TABLE 1 quality ratio of each component in each example and each component in comparative example
Performance test 1: keratinocyte gene expression assay
Caspase14(CASP14, aspartase proteolytic enzyme) is a key enzyme involved in the terminal differentiation of epidermal cells and the formation of skin barrier, and can be used as an index for evaluating the state of skin barrier function.
And detecting the regulation of the composition on the target gene expression level by adopting a real-time fluorescent quantitative PCR method.
Negative control (no composition NT); positive control (containing 0.01% mass concentration of hyaluronic HA); in the culture media of examples 1 to 7 and comparative examples 1 to 3, the mass concentration of each group was 0.1%.
HaCaT cells 3 x 105And inoculating the cells to a 6-well plate, culturing for 24h, and carrying out adherent growth on the cells. Samples were prepared as 1X gradient dilution spots of 2 total concentrations, 2ml per well added to the cell plate, 5% CO at 37 ℃2After the constant-temperature incubator is continuously incubated for 24h, cells are collected, total RNA of the cells is extracted by using a PureLink mini RNA extraction kit, the concentration of nucleic acid is quantitative, 200 ng of RNA in each tube is used as a template, and the relative expression quantity (20 mu l system) of each gene is detected by performing Real-Time quantitative fluorescence PCR (reverse transcription + Real Time-PCR) through a Taqman one-step reaction.
Calculating the formula:
Fold change＝2-ΔΔCT；
- Δ Δ CT ═ group to be tested (CT target gene-CT reference gene) control group.
In this experiment, the target gene was CASP14 and the reference gene was 1, GAPDH.
The test results are shown in the attached figure 1:
1) the CASP14 gene expression activity of the compositions of examples 1-3 is increased with the increase of lactobacillus fermentation lysate, and has a certain dose-effect relationship;
2) the CASP14 gene expression of the compositions of examples 2, 4 and 5 is increased along with the increase of the beta-glucan fermentation liquor, and has a certain dose-effect relationship;
3) the CASP14 gene expression of the compositions of examples 4, 6 and 7 increased with increasing aqueous polyquaternium-51 and then decreased, presumably due to the penetration promoting effect of polyquaternium-51, and when the content exceeds a certain amount, the active ingredient was accelerated to pass through the epidermis layer and reach the dermis layer;
4) the CASP14 gene expression of the embodiment 6 is obviously superior to that of the comparative example 1, which shows that the CASP14 gene expression is positively promoted by utilizing the endogenous beta-1, 3-endoglucanase for enzymolysis at the later stage of fermentation;
5) the CASP14 gene expression of example 6 is superior to that of comparative example 2, which shows that the high pressure homogenization method can effectively promote the release of active substances in the lactobacillus lysate, and is beneficial to the CASP14 gene expression;
6) the CASP14 gene expression of example 6 is superior to that of comparative example 3, which shows that polyquaternium-51 has a certain promotion effect on CASP14 gene expression.
In summary, the skin rejuvenating, anti-aging micro-ecological composition of example 6 is preferred.
Performance test 2: skin gloss and skin wrinkle improvement test
Screening of 30 healthy volunteers aged 25-40 years. The skin gloss was measured using the skin rejuvenating, anti-aging emulsion containing the microecological composition of example 6 (1% and 3% of the composition, respectively), applied to the whole face, 1 time in the morning and evening, each time about 3-4 soybeans in size, for 4 consecutive weeks, using the skinnglossmeter, and the skin wrinkles using the Antera 3D, and the results are shown in FIGS. 2-3.
It will be understood by those skilled in the art that the foregoing is only exemplary of the present invention, and is not intended to limit the invention, which is intended to cover any variations, equivalents, or improvements therein, which fall within the spirit and scope of the invention.
Claims (10)
1. A skin rejuvenation and anti-aging micro-ecological composition which comprises a lactobacillus fermentation lysate, a β -glucan fermentation broth, and polyquaternium-51.
2. The skin rejuvenating and anti-aging micro-ecological composition as claimed in claim 1 wherein said lactic acid bacteria fermentation lysate is a lactic acid bacteria fermentation lysate from which cell wall particles and cytoplasmic protein components are removed.
3. The skin rejuvenating and antiaging microecological composition as claimed in claim 1 wherein said lactobacillus fermentation lysate is prepared by the steps of: inoculating lactobacillus in MRS liquid culture medium in an inoculum size of 0.5-5%, culturing in a shaking table at 35-45 deg.C and 200rpm for 1-3 days, pasteurizing, centrifuging, collecting lactobacillus thallus, washing with sterilized distilled water, and suspending the thallus in sterilized distilled water; taking suspended bacteria liquid, performing wall breaking treatment by using a high-pressure homogenizer at the temperature of 70-90 ℃ and the pressure of 400-600bar, breaking the wall, centrifuging, collecting supernatant, standing for 12-24h at the temperature of 4-6 ℃, filtering, and collecting filtrate to obtain the lactobacillus fermentation lysate.
4. The skin rejuvenation and anti-aging micro-ecological composition as defined in claim 1 wherein the β -glucan fermentation broth is prepared by enzymatic hydrolysis of polysaccharide molecular weight using a fermentation system endogenous β -1, 3-glucanase enzyme at the late stage of fermentation.
5. The skin rejuvenating and anti-aging micro-ecological composition as set forth in claim 1, wherein the β -glucan fermentation broth is prepared by:
a. culturing Schizophyllum commune strains for 6-8 days at 25-30 ℃, inoculating mycelia from an inclined test tube, transferring into a seed bottle with a liquid loading capacity of 100-;
b. transferring the second-stage seed culture solution into a fermentation tank with an inoculum size of 2-5%, adjusting the internal pressure of the tank to be 0.02-0.04MPa, and controlling the ventilation quantity according to the volume of the material liquid: the volume of air is 1: 0.5-1.2, stirring speed of 100-200r/min, temperature of 25-30 ℃, culturing for 3.5-4.5 days, centrifuging to remove thalli, performing polysaccharide molecular weight enzymolysis by using endogenous beta-1, 3-glucanase of a fermentation system, temperature of 37-43 ℃, pH of 5.3-5.8, enzymolysis time of 18-30h, filtering, and collecting filtrate to obtain beta-glucan fermentation liquor.
6. The skin rejuvenating and anti-aging micro-ecological composition as set forth in claim 1 wherein the polysaccharide content of said β -glucan broth is from 0.4 to 1.2%; preferably, the polyquaternium-51 is a polyquaternium-51 aqueous solution with the mass concentration of 2-6%; preferably, the lactobacillus fermentation lysate: beta-glucan fermentation broth: the mass ratio of polyquaternium-51 is 1-5: 0.5-2: 1-3; preferably, the lactobacillus fermentation lysate: beta-glucan fermentation broth: the mass ratio of polyquaternium-51 is 2.5-3.5: 0.8-2: 1-3.
7. A method of preparing a skin rejuvenating and anti-aging micro ecological composition as claimed in any one of claims 1 to 6 which comprises the steps of:
(1) culturing Schizophyllum commune strains for 6-8 days at 25-30 ℃, inoculating mycelia from an inclined test tube, transferring into a seed bottle with a liquid loading capacity of 100-;
(2) transferring the second-stage seed culture solution into a fermentation tank with an inoculum size of 2-5%, adjusting the internal pressure of the tank to be 0.02-0.04MPa, and controlling the ventilation quantity according to the volume of the material liquid: the volume of air is 1: 0.5-1.2, stirring at a speed of 100-200r/min, culturing at 25-30 ℃ for 3.5-4.5 days, centrifuging to remove thallus, performing polysaccharide molecular weight enzymolysis by using endogenous beta-1, 3-glucanase of a fermentation system at a temperature of 37-43 ℃, a pH value of 5.3-5.8 and an enzymolysis time of 18-30h, filtering, and collecting filtrate to obtain beta-glucan fermentation liquor for later use;
(3) inoculating lactobacillus in MRS liquid culture medium in an inoculum size of 0.5-5%, culturing in a shaking table at 35-45 deg.C and 200rpm for 1-3 days, pasteurizing, centrifuging, collecting lactobacillus thallus, washing with sterilized distilled water, and suspending the thallus in sterilized distilled water;
(4) taking suspended bacteria liquid, performing wall breaking treatment by using a high-pressure homogenizer at the temperature of 70-90 ℃ and the pressure of 400-600bar, breaking the wall, centrifuging, collecting supernatant, standing for 12-24h at the temperature of 4-6 ℃, filtering, and collecting filtrate to obtain a lactobacillus fermentation lysate for later use;
(5) uniformly mixing the lactobacillus fermentation lysate, the beta-glucan fermentation liquor and the polyquaternium-51 aqueous solution according to the proportion to obtain the skin repairing and anti-aging microecological composition.
8. The method for preparing the skin-rejuvenating and antiaging microecological composition as claimed in claim 7, wherein the culture medium for the strain culture in step (1) comprises glucose 25-35g/L, yeast extract 2-4g/L, KH2PO4 0.8-1.2g/L，MgSO4·7H2O0.4-0.6 g/L, pH 4-5, adding 1.5-2.5% agar; preferably, the components of the culture medium of the fermentation tank in the step (2) are 25-35g/L of glucose, 2-4g/L of yeast extract and KH2PO4 0.8-1.2g/L，MgSO4·7H20.4-0.6g/L of O, 0.04-0.06% of defoaming agent and 4-5 of pH; preferably, the wall breaking is performed by wall breaking for 4-6min, and repeating for 3-5 times at intervals of 4-6 min.
9. Use of the skin rejuvenating, anti-aging eco-composition as claimed in any one of claims 1 to 6 for the preparation of a cosmetic or as a cosmetic adjuvant, additive.
10. The use of the skin rejuvenating and antiaging composition as claimed in claim 9 wherein the cosmetic is a lotion, essence, emulsion or cream.
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