CN114246655A - Oocyte collector - Google Patents
Oocyte collector Download PDFInfo
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- CN114246655A CN114246655A CN202111257960.2A CN202111257960A CN114246655A CN 114246655 A CN114246655 A CN 114246655A CN 202111257960 A CN202111257960 A CN 202111257960A CN 114246655 A CN114246655 A CN 114246655A
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- 210000000287 oocyte Anatomy 0.000 title claims abstract description 108
- 210000004681 ovum Anatomy 0.000 claims abstract description 71
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 58
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 58
- 238000007789 sealing Methods 0.000 claims abstract description 48
- 238000012360 testing method Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 230000001681 protective effect Effects 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 3
- 210000003101 oviduct Anatomy 0.000 claims description 3
- 238000012858 packaging process Methods 0.000 claims description 3
- 241000973497 Siphonognathus argyrophanes Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 9
- 238000000338 in vitro Methods 0.000 description 6
- 230000036512 infertility Effects 0.000 description 4
- 208000000509 infertility Diseases 0.000 description 4
- 231100000535 infertility Toxicity 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000003139 buffering effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 238000002604 ultrasonography Methods 0.000 description 3
- 238000005452 bending Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 210000001733 follicular fluid Anatomy 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 210000001215 vagina Anatomy 0.000 description 2
- 206010013654 Drug abuse Diseases 0.000 description 1
- 206010018852 Haematoma Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 101000928179 Homo sapiens Agouti-related protein Proteins 0.000 description 1
- 206010033266 Ovarian Hyperstimulation Syndrome Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 102000055839 human AGRP Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 208000011117 substance-related disease Diseases 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
- A61B17/3403—Needle locating or guiding means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
- A61B17/3403—Needle locating or guiding means
- A61B2017/3413—Needle locating or guiding means guided by ultrasound
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- Surgery (AREA)
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- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Heart & Thoracic Surgery (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Clinical Laboratory Science (AREA)
- Medical Informatics (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pathology (AREA)
- Transplantation (AREA)
- Gynecology & Obstetrics (AREA)
- Pregnancy & Childbirth (AREA)
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Abstract
The invention discloses an oocyte collector, which comprises a negative pressure suction tube, a puncture needle handle and an oocyte collecting tube, wherein the puncture needle, the puncture needle handle and the oocyte collecting tube are sequentially connected; the end parts of the oocyte collecting pipe and the negative pressure suction pipe are fixed inside the sealing plug, one end of the oocyte collecting pipe is embedded inside the sealing plug, and the sterile collecting test tube is clamped on the sealing plug and used for collecting sucked oocytes; an oocyte release cavity is arranged at the tail end of the oocyte collecting pipe and is communicated with the cavity of the oocyte collecting pipe. The ovum enters the oocyte collecting tube through the cavity of the convex oocyte collecting tube, so that the ovum can fall downwards along the wall of the test tube, the impact force of the ovum when entering liquid is reduced, and the deformation and damage of the ovum are reduced. The oocyte release cavity in the sealing plug can release and buffer the ovum, so that the ovum forms a larger dropping ball, the ovum is protected in the falling process, and meanwhile, the ovum is prevented from staying in the pipeline.
Description
Technical Field
The invention relates to the technical field of ovum taking surgical instruments, in particular to an oocyte collector.
Background
Modern people defer pregnancy plans for various reasons, so that the incidence rate of infertility tends to increase year by year, the advanced age is one of the reasons for the occurrence of the infertility, in addition, the infertility is also influenced by factors such as environment, self pressure, drug abuse and the like, the incidence rate also tends to increase year by year, and more infertility patients are treated by assisted reproduction technology, particularly in vitro fertilization. Currently, human ART mainly includes intrauterine fertilization, in vitro fertilization-embryo transfer, artificial insemination, in vitro maturation of ova, injection of single sperms of oocyte plasma, freezing and thawing of ova, sperm and embryo, genetic diagnosis before transfer, and the like. The treatment aim of a patient is to obtain clinical pregnancy and healthy offspring, and the aim is to obtain high-quality ova, improve the fertility rate and finally obtain more high-quality embryos on the premise of achieving the aim, but the quality of the ova and the embryos can be influenced in a plurality of links such as clinical medication, an ovum taking operation, embryo culture-transplantation and the like. In vitro fertilization-embryo transfer (IVF-ET) cycle usually performs artificial ovarian hyperstimulation, which aims to stimulate a plurality of primordial follicles to develop synchronously to obtain more high-quality ova so as to obtain high-quality embryos for selection and transfer, and finally improve the clinical pregnancy rate.
The ovum taking operation is the first step of in vitro operation in the in vitro fertilization-embryo transplantation technology treatment, and the used equipment for taking the ovum consists of an ovum taking needle, a negative pressure suction apparatus, a negative pressure pump connecting pipe between the ovum taking needle and the negative pressure suction apparatus and a disposable sterile test tube. The ovum picking operation is to suck mature oocytes from an ovarian tissue through a vagina under the guidance of B ultrasonic, is an intermediate link from the oocytes leaving a human body to a laboratory incubator for culture, and because the operation time is long, the oocytes are easily influenced by various factors, the physiological function of the oocytes can be changed, and the ovum picking process is influenced by the proficiency of an operator and the stability of the whole ovum picking channel.
As shown in fig. 1, the conventional ovum fetching device generally comprises a puncture needle, a collection tube 5 ', a negative pressure absorption tube 6' and a sealing plug 7 ', wherein the ovum directly enters the test tube through the collection tube 5', which causes inevitable damage in the ovum collection process, and therefore, how to reduce the impact force of the ovum in the collection process becomes a problem which needs to be solved at present.
Disclosure of Invention
The invention aims to provide an oocyte collector, which can enable ova to fall downwards along the wall of a test tube when the ova enter the test tube through a collecting tube cavity in an ova taking operation, reduce the impact force of the ova when the ova fall into liquid and reduce the deformation damage of the ova.
In order to realize the aim, the invention provides an oocyte collector, which comprises a negative pressure suction tube, a puncture needle handle and an oocyte collecting tube, wherein the puncture needle, the puncture needle handle and the oocyte collecting tube are sequentially connected; the end parts of the oocyte collecting pipe and the negative pressure suction pipe are embedded and fixed in the sealing plug, and the sterile collecting test tube is clamped on the sealing plug and is used for collecting the sucked oocytes; inside the sealing plug, the end of oocyte collecting pipe is provided with oocyte release chamber, oocyte release chamber oocyte collecting pipe cavity intercommunication.
According to the invention, the joint of the oocyte release cavity and the oocyte collecting pipe cavity is also provided with an injector connecting port, the injector connecting port is connected with an injection pipe, and the oocyte collecting pipe and the puncture needle are injected with liquid to clean the oviduct duct before taking the ovum.
According to the invention, a concave negative pressure tube cavity is formed at the end part of the negative pressure suction tube positioned in the sealing plug, and the tail end of the negative pressure suction tube is also connected with a negative pressure suction tube connecting seat which is used for being communicated with a negative pressure device.
Preferably, relative to the small-diameter bottom plane of the sealing plug, the negative pressure tube cavity extends to the tube orifice of the sterile collection tube and is of a concave structure.
According to the invention, the negative pressure suction tube and the collecting tube are similar in shape and are arranged in parallel, and the tail end of the negative pressure suction tube is connected with the negative pressure suction connecting seat for being communicated with the negative pressure device.
According to the invention, relative to the small-diameter bottom plane of the sealing plug, the oocyte collection tube cavity extends towards the interior of the sterile collection test tube and is in a convex structure; an inclined opening structure is formed at the part of the oocyte collecting pipe cavity facing outwards and close to the pipe wall of the sterile collecting test pipe, so that the ovum is protected from falling downwards along the pipe wall of the sterile collecting test pipe connected with the oocyte collecting pipe cavity.
According to the invention, the inclination of the inclined port structure is 20-45 degrees by taking the axial direction of the sealing plug as a reference. Preferably, the inclination of the inclined port structure is 30-40 °, such as 15 °, 25 °, 35 °, 45 °, 55 ° or any point in a range of values consisting of any two points.
According to the invention, the joint of the oocyte collecting tube cavity and the oocyte release cavity is in a concave structure.
According to the invention, the tube orifice of the sterile collection tube is clamped at the upward one third position of the bottom of the sealing plug.
According to the invention, the puncture needle collecting device also comprises a puncture needle protecting sleeve and a collecting pipe protecting sleeve; the puncture needle protective sleeve is used for protecting the puncture needle in the egg taking and packaging process; the collecting tube protective sleeve is arranged at the joint of the oocyte collecting tube and the puncture needle holding handle, so that the oocyte collecting tube is prevented from being blocked due to bending, and the ovum is blocked or lost. Preferably, the puncture needle holder is in a cylindrical shape with a thin middle part and thick two ends.
According to the invention, the collector tube consists of a multi-layer sleeve, which is formed from a material with a heat exchange medium.
The invention has the beneficial effects that:
1) in the prior art, the ovum directly enters the test tube through the collecting tube, so that the damage to the ovum is large, but the collecting tube cavity with a convex structure is arranged on the sealing plug of the invention, and the ovum enters the test tube through the collecting tube cavity, so that the ovum can fall downwards along the wall of the test tube, the impact force of the ovum when falling into liquid is reduced, the deformation damage of the ovum is reduced, and the ovum is protected and collected. In addition, the ovum taking device sealing plug is also provided with a negative pressure tube cavity with a concave structure, so that the backflow of liquid in the negative pressure suction process can be prevented, and the device is protected; while allowing each tube to be filled with a greater volume of liquid.
2) The sealing plug is also provided with an ovum release cavity structure for releasing and buffering the ovum, so that the ovum forms a larger droplet ball, the ovum is protected in the falling process, and the ovum is prevented from staying in a pipeline. And still design the syringe connector in the sealing plug for connect syringe and push main device and push the flush fluid in order to push the ovary, make things convenient for doctor's operation.
Drawings
FIG. 1 is a schematic structural diagram of a partial sealing plug of an oocyte collector in the prior art;
FIG. 2 is a schematic diagram of the overall structure of the oocyte collector of the present invention;
FIG. 3 is a schematic structural diagram of the partial sealing plug of the oocyte collector of the present invention.
In the figure: 1-puncture needle protective sleeve; 2, puncturing needle; 3-puncture needle handle holding; 4-connecting a protective sleeve; 5-oocyte collection tube; 6-negative pressure suction tube; 7-a sealing plug; 8-negative pressure suction connecting seat; 9-oocyte release chamber; 10-negative pressure tube cavity; 11-oocyte collection tube cavity; 12-syringe connection port; 110-inclined port configuration.
Detailed Description
The following detailed description of embodiments of the invention, but the invention can be practiced in many different ways, as defined and covered by the claims.
As shown in figures 2 and 3, the invention provides an oocyte collector, which comprises a negative pressure suction tube 6, and a puncture needle 2, a puncture needle handle 3 and an oocyte collecting tube 5 which are connected in sequence. The end parts of the oocyte collecting pipe 5 and the negative pressure suction pipe 6 are fixed inside the sealing plug 7, one end of the oocyte collecting pipe 5 is embedded into the sealing plug 7, and the sterile collection test tube is clamped on the sealing plug 7 and used for collecting the sucked oocytes. An oocyte release cavity 9 is arranged inside the sealing plug 7 and at the tail end of the oocyte collecting pipe 5, and the oocyte release cavity 9 is communicated with the oocyte collecting pipe cavity 11.
The sealing plug 7 is used for connecting the oocyte collecting tube 5, the negative pressure suction tube 6 and the sterile collecting test tube, namely, one end of the oocyte collecting tube 5 is connected with the puncture needle 2, and the other end is connected with the sealing plug 7, so that a channel is provided for taking out the ovum. Preferably, the oocyte collecting tube 5 is composed of a plurality of layers of sleeves, and the sleeves are made of materials with heat exchange media and have a heat preservation function.
In the prior art, oocytes directly enter a sterile collection test tube through an oocyte collection pipeline, so that the damage to the ova is large, and in order to avoid the problem, the oocyte collection tube cavity 11 with a protruding structure is arranged in the sealing plug 7. The bulge structure has the bevel opening design towards the outer part (namely the aseptic collection test tube direction), so that the oocyte can descend downwards along the aseptic collection test tube wall, the impact force when the oocyte descends into liquid is reduced, the deformation damage of the oocyte is reduced, and the oocyte is protected. The sealing plug is also provided with an oocyte release cavity 9 structure which is used for releasing and buffering the ovum, so that the ovum forms a larger droplet ball, the ovum is protected in the falling process, and the ovum is prevented from staying in a pipeline.
According to the invention, a negative pressure tube cavity 10 with a larger space is formed at the end part of the negative pressure suction tube 6 positioned in the sealing plug 7, the tail end of the negative pressure suction tube 6 is also connected with a negative pressure suction tube connecting seat 8 for communicating with a negative pressure absorption device, the other end of the negative pressure suction tube connecting seat is connected with the sealing plug 7, and the negative pressure suction tube connecting seat 8 is connected with the negative pressure suction tube 6 and the negative pressure absorption device. Relative to the small diameter bottom plane of the sealing plug 7, the negative pressure tube cavity 10 extends towards the tube orifice of the sterile collection tube and is concave. That is, the bottom end of the oocyte collection tube cavity 11 is below the level of the bottom surface of the sealing plug 7, while the negative pressure tube cavity 10 is above the level of the bottom surface of the sealing plug 7.
The invention arranges a negative pressure tube cavity 10 at the tail end of a negative pressure suction tube 6, which aims to prevent liquid from flowing backwards in the negative pressure suction process and protect equipment; the negative pressure tube cavity 10 is designed in a concave manner relative to the sealing plug 7, and the negative pressure tube cavity with the concave structure reduces the occupied volume in the sterile collection test tube, so that each sterile collection test tube is filled with more liquid.
Preferably, the negative pressure aspiration tube 6 is shaped similarly to the oocyte collection tube 5 and arranged in parallel, both in a "concave" configuration.
In a preferred embodiment of the invention, as shown in FIG. 3, the oocyte collection tube cavity 11 extends towards the interior of the sterile collection tube in a convex configuration relative to the bottom plane of the sealing plug 7 (the end of the sealing plug with the smaller diameter); the part of the oocyte collecting pipe cavity 11, which faces the outer side and is close to the tube wall of the sterile collecting tube, is provided with an inclined opening structure 110 so as to protect the ovum from falling downwards along the tube wall of the sterile collecting tube connected with the oocyte collecting pipe cavity, reduce the impact force of the ovum when the ovum falls into liquid and avoid the ovum deformation damage. Meanwhile, the collecting pipe cavity 11 can be used as a collecting pipe for collecting the ovum.
Preferably, the inclination of the inclined port structure 110 is 30-40 °, for example, 15 °, 25 °, 35 °, 45 °, 55 ° or any point value in a range of values of two points, with the axial direction of the sealing plug 7 as a reference.
The sealing plug is provided with an oocyte release cavity 9 structure for releasing and buffering the ovum, so that the ovum forms a larger droplet ball, the ovum is protected in the falling process, and the ovum is prevented from staying in a pipeline. Preferably, the junction of the oocyte collection tube cavity 11 and the oocyte release chamber 9 is in a recessed U-shaped structure.
As shown in figure 3, a syringe connecting port 12 is also arranged at the joint of the oocyte release cavity 9 and the oocyte collecting pipe cavity 11, the syringe connecting port 12 is connected with a syringe, and cleaning fluid is injected into the oocyte collecting pipeline 5 and the puncture needle 2 before egg taking for cleaning the oviduct.
Preferably, the mouth of the sterile collection tube is snapped onto the bottom third of the sealing stopper 7.
In a preferred embodiment of the invention, as shown in figure 2, the oocyte collector further comprises a puncture needle protective sheath 1 and a collection tube protective sheath 4. The puncture needle protective sleeve 1 is used for protecting the puncture needle 2 in the egg taking and packaging process, and avoids packaging damage and product failure caused by exposure of the puncture needle 2. The collecting tube protective sleeve is arranged at the joint of the oocyte collecting tube 5 and the puncture needle handle 3, so that the oocyte collecting tube 5 is prevented from being blocked due to bending, and the ovum is blocked or lost.
Preferably, the puncture needle holder 3 is in a cylindrical shape with a thin middle part and thick two ends, and the shape is helpful for a doctor to hold the ovum taking needle, so that the doctor can conveniently operate in the operation.
The oocyte collector of the invention is mainly used for collecting ova by puncturing follicles under the guidance of abdominal or vaginal ultrasound, and the specific use method is as follows:
1) the puncture needle part is fixed on the puncture frame (when necessary), the negative pressure suction device is connected with the negative pressure suction connecting seat at the tail end of the negative pressure connecting pipe, and the sterile collection test tube is connected with the sealing plug.
2) Under the guidance of vaginal ultrasound, the puncture needle punctures the follicle through the vagina. When the puncture needle reaches the surface of the follicle, a proper force is applied after the puncture needle is stopped slightly, so that the puncture needle rapidly penetrates into the follicle cavity to obtain the ovum in the follicle, and the ovum enters the oocyte collecting tube through the inner cavity of the puncture needle; the front end of the puncture needle is provided with an ultrasonic developing device, so that the position of the puncture needle can be conveniently observed under the guidance of vaginal ultrasound.
3) Confirming that the needle tip is positioned in the follicular cavity, starting a negative pressure aspirator, and sucking follicular fluid until the follicle is collapsed. After the follicular fluid of one follicle is exhausted, the puncture needle is inserted into the adjacent larger follicle.
4) After the ovum is taken out, the puncture needle is withdrawn to the outside of the body. The pelvic cavity is scanned conventionally to check whether internal bleeding or hematoma is formed.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. An oocyte collector is characterized by comprising a negative pressure suction tube (6), and a puncture needle (2), a puncture needle handle (3) and an oocyte collecting tube (5) which are connected in sequence; the end parts of the oocyte collecting pipe (5) and the negative pressure suction pipe (6) are embedded and fixed in the sealing plug (7), and the opening end of the sterile collecting test tube is clamped on the sealing plug (7) and used for collecting the sucked oocytes;
inside sealing plug (7), the end of oocyte collecting pipe (5) is provided with oocyte release chamber (9), oocyte release chamber (9) and oocyte collecting pipe cavity (11) intercommunication.
2. The oocyte collector of claim 1, wherein a syringe connecting port (12) is further formed at the joint of the oocyte release cavity (9) and the oocyte collecting pipe cavity (11), the syringe connecting port (12) is connected with a syringe, and liquid is injected into the oocyte collecting pipe (5) and the puncture needle (2) to clean the oviduct duct before egg taking.
3. The oocyte collector according to claim 1, wherein a concave negative pressure tube cavity (10) is formed at the end part of the negative pressure suction tube (6) positioned inside the sealing plug (7), and a negative pressure suction tube connecting seat (8) is further connected to the tail end of the negative pressure suction tube (6) and is used for being communicated with a negative pressure device.
Preferably, relative to the small diameter bottom plane of the sealing plug (7), the negative pressure tube cavity (10) extends towards the tube orifice of the sterile collection test tube and is of a concave structure.
4. The oocyte collector of claim 1, wherein the negative pressure suction tube (6) and the oocyte collection tube (5) are similar in shape and are arranged in parallel, and the tail end of the negative pressure suction tube (6) is connected with a negative pressure suction connecting seat (8) for being communicated with a negative pressure device.
5. The oocyte collector of claim 1, wherein the oocyte collection tube cavity (11) extends towards the interior of the sterile collection tube and is in a convex structure relative to the small diameter bottom plane of the sealing plug (7); an inclined opening structure (110) is formed at the part of the oocyte collecting pipe cavity (11) facing to the outer side and close to the pipe wall of the sterile collecting test pipe so as to protect the ovum from falling downwards along the pipe wall of the sterile collecting test pipe connected with the oocyte collecting pipe cavity.
6. Oocyte collector according to claim 6, wherein the inclination of the inclined port structure (110) is 20-45 ° with the axial direction of the sealing plug (7) as a reference.
Preferably, the inclination of the inclined port structure (110) is 30-40 °, such as 15 °, 25 °, 35 °, 45 °, 55 ° or any point in a range of values consisting of any two points.
7. The oocyte collector of claim 6, wherein the joint of the oocyte collecting tube cavity (11) and the oocyte release cavity (9) is in a concave structure.
8. The oocyte collector of claim 1, wherein the tube mouth of the sterile collection tube is clamped at the upward third position of the bottom of the sealing plug (7).
9. The oocyte collector of claim 1, further comprising a puncture needle protective sleeve (1) and a collection tube protective sleeve (4); the puncture needle protective sleeve (1) is used for protecting the puncture needle (2) in the egg taking and packaging process; collecting pipe protective sheath (4) set up oocyte collecting pipe (5) with the junction of handle (3) is held to the pjncture needle, in order to avoid buckling and cause oocyte collecting pipe (5) are blockked up, cause the ovum to block or lose.
Preferably, the puncture needle holder (3) is in a cylindrical shape with a thin middle part and thick two ends.
10. The oocyte collector of claim 1, wherein the oocyte collection tube (5) is comprised of a multi-layer sleeve formed of a material having a heat exchange medium.
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CN202111257960.2A CN114246655B (en) | 2021-10-27 | 2021-10-27 | oocyte collector |
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CN202111257960.2A CN114246655B (en) | 2021-10-27 | 2021-10-27 | oocyte collector |
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CN114246655A true CN114246655A (en) | 2022-03-29 |
CN114246655B CN114246655B (en) | 2023-11-10 |
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Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
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