CN114224550A

CN114224550A - Experimental method for murine animals with orthodontic far-middle moving upper jaw central incisor

Info

Publication number: CN114224550A
Application number: CN202111508315.3A
Authority: CN
Inventors: 艾虹; 康晓宁; 阙洪瑞; 魏佳明; 王语旋
Original assignee: Third Affiliated Hospital Sun Yat Sen University
Current assignee: Third Affiliated Hospital Sun Yat Sen University
Priority date: 2021-12-10
Filing date: 2021-12-10
Publication date: 2022-03-25

Abstract

The invention discloses a mouse experimental method for orthodontic far-middle-moving upper anterior teeth, which comprises the following steps: s1, preparing an orthodontic distal movement upper anterior tooth retention groove: preparing a retaining sulcus of about 0.08mm within a physiological range of upper anterior labial enamel thickness; s2, establishing a mouse upper anterior tooth far and middle movement model by utilizing the shape memory characteristic of the orthodontic nickel-titanium wire and the orthodontic ligation technology. The invention successfully constructs a stable, tissue-free and simple mouse anterior tooth orthodontic movement model based on the nickel-titanium wire and ligation technology which are commonly used in clinic. The invention effectively fills the blank that no mouse orthodontic tooth moving model capable of meeting the experimental requirements exists at the present stage, solves the dilemma that animal experimental mechanism exploration is difficult to develop due to the absence of effective animal model guarantee to a great extent, reduces the difficulty and complexity of related experiments to a certain extent, continuously distributes the orthodontic force on teeth, effectively simulates clinical orthodontic behaviors, and can be popularized and used in a large scale.

Description

Experimental method for murine animals with orthodontic far-middle moving upper jaw central incisor

Technical Field

The invention relates to the technical field of animal experiments, in particular to a murine animal experiment method for orthodontic far-middle moving of maxillary central incisors.

Background

Currently, with the improvement of living and aesthetic levels, more and more people choose orthodontic treatment to correct the facial deformities such as the dentition irregularity, the protrusion and the retraction. The core of dentition correction is the controlled movement of teeth within the collar bone, for which reason many scholars have conducted extensive research on tooth movement. The factors influencing tooth movement are very many, the influence mechanism is different, and some implementation measures cannot be directly researched on a human body, so that an animal experiment becomes one of basic experiment methods for orthodontic tooth movement research.

The results of animal experiments are based on a good establishment of experimental models of animals, which are the first choice of experimental animals. From the related literature, it is known that rats are used as experimental animals in animal experiments for orthodontic tooth movement, and other selected animals include small pigs, rabbits, dogs, primates, and monkeys. Primates have been biochemically closer to humans than other animals, and except for the difference in dentition size, the number of teeth, the primate spaces present, and the anatomy of teeth and periodontal pockets are very similar to those of humans, and are ideal experimental animals. However, the price is expensive, and the product is not easy to purchase, so that large-scale experimental study is difficult to carry out. The same rat, miniature pig. Rabbits, dogs and the like are inconvenient to use as orthodontic models, have large body sizes, relatively difficult material taking and high cost, and corresponding transgenic mice are difficult to prepare. Whereas the mice were rodents, teeth 11/1, M3/3, and typically all of the maxillary central incisors were fully erupted 5 weeks after birth, and were single teeth. Except for the continuous growth of incisor tooth roots, the anatomical structures and the mutual relations of periodontal tissues such as tooth paste, alveolar bone and periodontal ligament are similar to those of a human body, and the mouse has the advantages of short growth cycle, easy feeding and low price, is suitable for large sample observation, can be used for preparing a transgenic mouse on the basis of the mouse, and is applied to the research of different mechanisms, so the mouse is the best experimental animal selected as an orthodontic tooth movement experiment. However, to date, there is no stable and reasonable model of orthodontic tooth movement in mice that meets experimental needs, thereby affecting the progress and depth of the relevant experiments. The moving model of the mouse anterior teeth is rather deficient, and the innovation of the invention breaks through the technical bottleneck.

The basic principle of the orthodontic movement of the teeth is that when a continuous external force is applied to the teeth, the continuous external force is generated when the pre-bent nickel-titanium wire recovers deformation, the continuous external force is transmitted to the teeth through the ligature wire tied and fixed on the teeth, and finally the teeth move along the direction of force, namely move towards far and middle. The key to this process is the stable retention of the ligature wire on the tooth.

The mouse orthodontics anterior teeth move by mutually resisting the middle incisors and drawing the maxillary middle incisors in a far-middle way. The current method is to advance the posterior teeth forward by means of a tension spring fixed to the anchorage anterior teeth. The posterior teeth are forwards moved by adhering the steel wire at the tail end of the spring to the posterior occlusal surface in China, but the mouse has the problems of small posterior tooth surface area, smooth steel wire, small contact area, incapability of being completely suitable for mice and the like, so that the existing adhesive force cannot meet the requirement of the forwards movement of the posterior teeth, the success evidence report of the model is insufficient, a plurality of questions exist in the design, and the model experiment cannot be repeated. Foreign reports have reported that the ligature wire is used to wrap the back tooth neck part through the back gum to connect the spring to move the back tooth forwards, and from the anatomical structure of the back tooth of the mouse, the design is also unreasonable, the tissue damage is great, and the experiment is difficult to repeat; and because the mouse oral cavity is narrow and small, it is comparatively hard to wear stainless steel ligature silk between first molar and second molar to influence going on smoothly of experiment. In addition, in the periodontal context, the appliance is also prone to inflammation of the first molar gingiva and alveolar bone resorption. Although the model of moving the posterior teeth of the mouse by using the tooth separating device can move the posterior teeth without tissue damage, the experimental requirement is difficult to meet due to the problems of fast mechanical attenuation of the tooth separating device and poor stability of distance rebound after moving. The anterior tooth movement model is only reported by a rat model, the injury is large, and the mouse anterior tooth orthodontic tooth movement model is almost the same at present.

Disclosure of Invention

Aiming at the prior art, the invention provides a murine animal experimental method for orthodontically and remotely moving incisors in the upper jaw, which has the advantages of simple and easy establishment process, small damage to teeth and good effect, and also provides a novel cast type mouse orthodontics device for remotely moving incisors in the upper jaw, which has the advantages of simple structure, difficult falling, strong controllability and small damage to teeth, and solves the problems that the appliance in the prior art is easy to fall off and damage to teeth.

The invention is realized by the following technical scheme:

an experimental method for murine animals orthodontically and distally moving maxillary central incisors comprises the following steps:

s1 preparation of orthodontic far-middle-moving maxillary central incisor correcting device

And carrying out anesthesia fixation on the mouse before carrying out anesthesia fixation and cleaning the tooth surface.

The specific method for anesthesia fixation is to use sodium pentobarbital for intraperitoneal injection for anesthesia, and fix the limbs and the head of a mouse in a supine position.

The labial retention groove of the fixed movable upper jaw central incisor is convenient for the fixation of the ligature wire.

S2 orthodontic distal movement maxillary middle incisor ring

The individualized belt rings are arranged on corresponding teeth, after the adhesion is finished, the U-shaped nickel-titanium wire is ligated and fixed on the retention groove by the ligation wire I, the target teeth are moved by applying force, and the animal model is successfully established.

The orthodontic far-middle-moving upper jaw central incisor correcting device prepared by the method comprises a retention groove, a ligation wire ring and a ligation wire I which are fixedly clamped with the upper jaw central incisor of a mouse in a matched mode, and a U-shaped nickel-titanium wire for stress application, wherein one of the two ligation wire rings surrounds the retention groove and penetrates through annular ligation, two ends of the U-shaped nickel-titanium wire are respectively connected with one ends of two ligation wires II, and the other ends of the ligation wires II are fixedly connected onto individual ring rings through traction holes.

The cast individual belt ring is cast from nichrome.

The personalized retention groove matched and fixedly sleeved with the incisors of the mice is arranged on the labial side of the incisors.

The traction ditch of the casting type individualized belt ring fixedly clamped with the upper incisor in the upper jaw of the mouse is arranged at the far middle side of the casting type individualized belt ring.

The ligature wire is made of chromium-nickel-titanium alloy, and the diameter of the ligature wire is 0.8 mm.

The invention has the beneficial effects that:

1. the experimental method for the murine animal for orthodontics far-middle moving incisors in the upper jaw and the orthodontic device reduce the falling rate of the orthodontic device and the damage to tissues in the orthodontic tooth moving process, so that the orthodontic force is more scientifically distributed on teeth to be beneficial to the directional traction of the teeth, and the smooth animal experiment is guaranteed.

2. Incisors adopt the individualized retention groove, are more attached to the appearance of incisors of mice, are bonded to the neck of the teeth by adopting the light-cured composite resin, are better in retention and not easy to fall off, and the problem that an incisor anchorage device in the prior art is easy to fall off is solved.

3. The moving tooth root ligates the individualized retaining groove around the lip according to the shape of the upper incisor, and the device can be well fixed in the oral cavity, thereby reducing the falling rate of the device.

4. The individual ligature wires I are respectively ligatured and bound with the U-shaped nickel-titanium wires, so that the stress application operation is simpler and more convenient.

5. In the existing method, the treatment of incisors needs to grind off a part of tooth bodies to prepare the retention groove, so that the injury to the tooth bodies of mice is large, and even the incisors are easy to break. The cast individual belt ring is adhered to the surface of the tooth body, so that the teeth are not damaged.

6. The personalized ligature wire ring of the mobile tooth is adhered to the surface of the tooth body by using an adhesive material, so that the damage to periodontal tissues during the first ligature wire threading and the stress application is avoided, and the performance of an experiment and the accuracy of an experiment result are facilitated.

7. The individualized ligation wire ring has large and uniform contact area with the tooth body, so that the correction force can be uniformly distributed on the tooth, and the stability of anchorage teeth and the directional traction of moving teeth are more facilitated.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

1. Fixing the ditch; 2. binding wires I; 3. a second ligature wire; 4. a nickel titanium wire.

Detailed Description

The invention is further illustrated by the following examples and figures.

Examples experimental methods for murine animals orthodontically and distally moving maxillary central incisors.

An animal model for orthodontic far-middle moving maxillary central incisor correction of a mouse is established, and the steps are as follows:

preparing an orthodontic far-middle-moving maxillary central incisor correcting device:

(1) performing anesthesia fixation on the mouse, performing intraperitoneal injection anesthesia by using sodium pentobarbital, and fixing the limbs and the head of the mouse in a supine position;

(2) preparation of anchorage teeth and individualized retention groove 1 for moving teeth a wax model was prepared on the model according to the labial side of the incisor in the upper jaw of the mouse.

And (II) establishing a full-anesthesia mouse for orthodontics far-middle moving maxillary middle incisor animal model, fixing limbs and head of the mouse in a supine position, cleaning tooth surfaces of the mouse, insulating moisture, bonding maxillary middle incisors, the retention groove 1 and the ligation wire ring anchorage on corresponding teeth by using light-cured composite resin, ligating and fixing the U-shaped nickel-titanium wire 4 in the retention groove 1 of the ligation wire I2 by using the ligation wire I2 after bonding is finished, applying force to move target teeth, and successfully establishing the animal model.

The orthodontic far-middle moving upper jaw central incisor correcting device prepared by the method comprises an individualized retention groove 1 matched and fixed with an upper jaw central incisor of a mouse and a U-shaped nickel-titanium wire 4 for applying force, wherein the retention groove 1 on the individualized front tooth lip side of a ligation wire ring and the mouse upper jaw central incisor are matched and fixed at the position close to the gingival margin on the lip side, a traction groove of an individualized wire ring matched and fixed with the mouse is arranged at the two ends of the U-shaped nickel-titanium wire 4 on the far-middle side of a cast individualized wire ring and connected with one end of two ligation wires II 3, and the other end of each ligation wire II 3 is fixedly connected to the individualized retention groove 1 through a traction hole.

The ligature wire I2 is made of chromium-nickel-titanium alloy, the diameter of the ligature wire I is 0.15mm, and an individualized ligature wire ring, a ligature wire I2, a U-shaped nickel-titanium wire 4 and a fixing groove 1 which are matched with incisors in the upper jaw of the mouse and fixedly clamped are fixedly sleeved with the incisors in the upper jaw of the mouse.

Claims

1. An experimental method for orthodontically and distally moving a mouse with upper anterior teeth is characterized by comprising the following steps:

s1 preparation of orthodontic distal movement upper anterior tooth retention groove (1)

An electric carving knife with a sharp lathe needle is utilized to grind a retention groove (1) with a transverse depth of about 0.08mm at the near gum end in the physiological range of the upper anterior labial enamel;

s2 establishment of orthodontics far-middle-moving maxillary central incisor animal model

Cleaning the tooth surface of a mouse, insulating moisture, adhering an individualized ligature wire ring of an incisor to a corresponding upper retaining groove (1) of the tooth by using light-cured composite resin, fixing a nickel-titanium wire (4) in the retaining groove (1) in a U shape with equal length by using a ligature wire I (1) after adhesion is finished, applying force, and successfully establishing an animal model.

2. The experimental method for murine animals with orthodontic distal upper anterior teeth movement as claimed in claim 1, wherein the anesthetic fixation is performed before the mice are anesthetized to clean the tooth surface.

3. The experimental method for the murine orthodontics capable of moving upper anterior teeth far away is characterized in that the specific method for anesthesia fixing is to use pentobarbital sodium 50mg/kg for intraperitoneal injection anesthesia, and to fix the limbs and the head of the mouse in a supine position.

4. The orthodontic appliance for the far and middle moving upper anterior teeth is characterized by comprising an individualized retention groove (1) matched and fixedly sleeved with incisors of mice, individualized ligation wire rings matched and fixedly clamped with the individualized retention groove and two ends of a nickel-titanium wire (4), wherein the two individualized ligation wire rings are respectively penetrated by another ligation wire II (3), and two ends of a U-shaped nickel-titanium wire (4) are respectively connected and retained with the two ligation wire II (3).

5. The retention groove (1) of claim 4 having a diameter of 0.08 mm.

6. The personalized mouse anterior tooth distal movement orthodontic appliance according to the claim, wherein the mouse incisor fit fixing set is arranged on the labial proximal teeth neck of the personalized ligation wire ring.

7. A section of nickel titanium wire (4) with the diameter of 0.15mm is pre-bent according to claim 5, two ends of the nickel titanium wire are respectively fixed in the front tooth far parts on two sides through a ligature wire I (2) to form a U shape, and the nickel titanium wire (4) is required to be attached to the palate part as much as possible. If necessary, the nickel-titanium wire (4) can be coated with fluid resin at the fixing ligation part and then is photo-cured to assist the fixation.