CN114212945A - Preparation method of low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats - Google Patents
Preparation method of low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats Download PDFInfo
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- CN114212945A CN114212945A CN202210159555.5A CN202210159555A CN114212945A CN 114212945 A CN114212945 A CN 114212945A CN 202210159555 A CN202210159555 A CN 202210159555A CN 114212945 A CN114212945 A CN 114212945A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/34—Treatment of water, waste water, or sewage with mechanical oscillations
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/48—Treatment of water, waste water, or sewage with magnetic or electric fields
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/68—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water
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Abstract
The invention relates to the technical field of hydrogen-rich water, in particular to a preparation method of low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats. Which comprises the following steps: adding purified water into the preparation container, sealing, and pumping out air in water by a vacuum pump; carrying out low-frequency resonance on the purified water by a resonance instrument to prepare low-frequency water; then introducing hydrogen into the low-frequency water, adding a mineral additive, pressurizing by a booster pump, and instantly relieving pressure to obtain low-frequency hydrogen-enriched water; in the preparation method of the low-frequency hydrogen-rich water capable of reducing the blood sugar of the diabetic rat, the hydrogen-rich water can neutralize acid metabolic wastes and lighten acid constitution, so that the condition of diabetes can be alleviated, and complications can be reduced.
Description
Technical Field
The invention relates to the technical field of hydrogen-rich water, in particular to a preparation method of low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats.
Background
Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by a defect in insulin secretion or an impaired biological action, or both. The chronic hyperglycemia results in chronic damage and dysfunction of various tissues, particularly eyes, kidneys, heart, blood vessels and nerves. In recent years, the incidence of diabetes has been increasing worldwide, and its complications seriously affect the physical and mental health of patients.
Diabetes is of great concern for damage to the reproductive system of patients, including: reproductive organ structure, reproductive hormone synthesis and secretion, germ cell generation and function, sexual function and progeny health, etc., especially the pathological mechanisms of their injury, in which dysregulation of hormone, cellular and molecular levels is involved. Therefore, the research on the method for reducing blood sugar of the diabetic has important clinical significance for the prevention, diagnosis and treatment of diabetes.
The main complications of diabetes are cardiovascular diseases, foot ulceration, retinopathy and renal failure, and meanwhile, the risks of stroke and amputation are increased, and the quality of life of a patient is seriously affected by the rise of blood sugar.
Disclosure of Invention
The invention aims to provide a preparation method of low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats, so as to solve the problems in the background technology.
In order to achieve the aim, the invention provides a preparation method of low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats, which comprises the following steps:
s1, adding purified water into the preparation container, sealing, and pumping out air in the water through a vacuum pump;
s2, carrying out low-frequency resonance on the purified water through a resonance instrument to obtain low-frequency water;
s3, introducing hydrogen into the low-frequency water, pressurizing by a booster pump, instantly relieving pressure to obtain low-frequency hydrogen-enriched water, and finally filling and sealing;
the mineral additive in the resonance instrument comprises the following raw materials: the mineral additives obtain energy in the magnetic field of the electromagnet, and the purified water is activated under the combined action of the vibration frequency of the oscillator and the photoelectric radiation of the light-emitting diode, so that the structure of water molecular groups is changed, and the quality of liquid water is improved.
As a further improvement of the technical scheme, the raw materials of the mineral additive comprise 2-10 parts of calcium chloride, 2-10 parts of calcium sulfate, 1-10 parts of magnesium chloride, 2-10 parts of magnesium sulfate, 6-10 parts of sodium bicarbonate and 6-10 parts of potassium carbonate.
As a further improvement of the technical scheme, in S1, the vacuum pumping time of the vacuum pump is 40-60min, which is long and can affect the concentration of hydrogen, so that the concentration of hydrogen in the purified water is significantly increased.
As a further improvement of the technical scheme, in the S2, the resonance frequency of the resonance instrument is 10-15hz, and low-frequency resonance water, namely activated water, has the function of reducing the risk of cancer. The blood viscosity is improved, and the incidence of cardiovascular and cerebrovascular diseases is reduced; regulating the pH value of the human body; inhibiting harmful bacteria and viruses; increasing cellular oxygen content; the sterilization effect is strong, and the immunity of the human body is enhanced; the frequency of human body cells is close to that of the human body cells, and the communication of the human body cells is enhanced; the antioxidant effect is strong; the higher the water resonance frequency, the poorer the water quality, and the lower the frequency, the better the water quality, so the low-frequency resonance water has better water quality than the common water.
As a further improvement of the technical scheme, in the S2, the low-frequency resonance time is 20-30min, so that the mixing efficiency of the mineral additive, the hydrogen and the water is improved.
As a further improvement of the technical scheme, in the step S3, the time for introducing the hydrogen is 20-40min, and the time for introducing the hydrogen is longer, so that the concentration of the hydrogen can be influenced, and the concentration of the hydrogen in the purified water is obviously improved.
As a further improvement of the technical scheme, in the S3, the pressure of the booster pump is 0.1-0.5 MPa.
As a further improvement of this embodiment, in S3, the filling amount of the low-frequency hydrogen-rich water is full, and when the low-frequency hydrogen-rich water is not full, the hydrogen concentration in the water decreases rapidly due to air contained in the air.
Compared with the prior art, the invention has the beneficial effects that:
1. in the preparation method of the low-frequency hydrogen-rich water capable of reducing the blood sugar of the diabetic rat, the hydrogen-rich water can neutralize acid metabolic wastes and lighten acid physique, so that the condition of diabetes can be alleviated, and the occurrence of complications can be reduced.
2. In the preparation method of the low-frequency hydrogen-rich water capable of reducing the blood sugar of the diabetic rat, the mineral additive can obtain energy in the magnetic field of the electromagnet by a low-frequency resonance method, and the purified water is activated under the combined action of the vibration frequency of the oscillator and the photoelectric radiation of the light-emitting diode, so that the structure of water molecular groups is changed, and the prepared hydrogen-rich water has better quality.
Drawings
FIG. 1 is an overall flow diagram of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Embodiment 1 a method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats comprises the following steps:
1. adding purified water into the preparation container, sealing, and pumping out air from water via vacuum pump for 40 min;
2. carrying out 10hz low-frequency resonance on the purified water by a resonance instrument to obtain low-frequency water, wherein the low-frequency resonance time is 20 min;
3. then introducing hydrogen into the low-frequency water, pressurizing by a booster pump for 0.1MPa, introducing high-pressure hydrogen for 40min, instantly relieving pressure to obtain low-frequency hydrogen-enriched water, and filling and sealing the low-frequency water in a bottle;
the raw materials of the mineral additive in the resonance instrument comprise 2 parts of calcium chloride, 2 parts of calcium sulfate, 1 part of magnesium chloride, 2 parts of magnesium sulfate, 6 parts of sodium bicarbonate and 6 parts of potassium carbonate.
Embodiment 2 a method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats comprises the following steps:
1. adding purified water into the preparation container, sealing, and pumping out air from water by vacuum pump for 45 min;
2. carrying out 12hz low-frequency resonance on the purified water by a resonance instrument to obtain low-frequency water, wherein the low-frequency resonance time is 23 min;
3. then introducing hydrogen into the low-frequency water, pressurizing by a booster pump for 0.2MPa, introducing high-pressure hydrogen for 35min, instantly relieving pressure to obtain low-frequency hydrogen-enriched water, and filling and sealing the low-frequency water in a bottle;
the raw materials of the mineral additive in the resonance instrument comprise 4 parts of calcium chloride, 4 parts of calcium sulfate, 4 parts of magnesium chloride, 4 parts of magnesium sulfate, 7 parts of sodium bicarbonate and 7 parts of potassium carbonate.
Embodiment 3 a method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats comprises the following steps:
1. adding purified water into the preparation container, sealing, and pumping out air from water via vacuum pump for 50 min;
2. carrying out 13hz low-frequency resonance on the purified water by using a resonance instrument to obtain low-frequency water, wherein the low-frequency resonance time is 25 min;
3. then introducing hydrogen into the low-frequency water, pressurizing by a booster pump for 0.3MPa, introducing high-pressure hydrogen for 30min, instantly relieving pressure to obtain low-frequency hydrogen-enriched water, and filling and sealing the low-frequency water in a bottle;
the raw materials of the mineral additive in the resonance instrument comprise 6 parts of calcium chloride, 6 parts of calcium sulfate, 6 parts of magnesium chloride, 6 parts of magnesium sulfate, 8 parts of sodium bicarbonate and 8 parts of potassium carbonate.
Embodiment 4 a method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats comprises:
1. adding purified water into the preparation container, sealing, and pumping out air from water by vacuum pump for 55 min;
2. carrying out 14hz low-frequency resonance on the purified water by using a resonance instrument to obtain low-frequency water, wherein the low-frequency resonance time is 27 min;
3. then introducing hydrogen into the low-frequency water, pressurizing by a booster pump for 0.4MPa, introducing high-pressure hydrogen for 25min, instantly relieving pressure to obtain low-frequency hydrogen-enriched water, and filling and sealing the low-frequency water in a bottle;
wherein the raw materials of the mineral additive in the resonance instrument comprise 8 parts of calcium chloride, 8 parts of calcium sulfate, 8 parts of magnesium chloride, 8 parts of magnesium sulfate, 9 parts of sodium bicarbonate and 9 parts of potassium carbonate.
Embodiment 5 a method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats comprises the following steps:
1. adding purified water into the preparation container, sealing, and pumping out air from water via vacuum pump for 60 min;
2. carrying out 15hz low-frequency resonance on the purified water by a resonance instrument to obtain low-frequency water, wherein the low-frequency resonance time is 30 min;
3. then introducing hydrogen into the low-frequency water, pressurizing by a booster pump for 0.5MPa, introducing high-pressure hydrogen for 20min, instantly relieving pressure to obtain low-frequency hydrogen-enriched water, and filling and sealing the low-frequency water in a bottle;
wherein the raw materials of the mineral additive in the resonance instrument comprise 10 parts of calcium chloride, 10g of calcium sulfate, 10 parts of magnesium chloride, 10 parts of magnesium sulfate, 10 parts of sodium bicarbonate and 10 parts of potassium carbonate.
Test example 1
The preparation method of the low-frequency hydrogen-rich water capable of reducing the blood sugar of the diabetic rat has the effect of reducing the blood sugar of the diabetic patient, and in order to verify the related technical scheme, the applicant performs the following tests:
selecting rats for test, selecting conventional drinking purified water and the low-frequency hydrogen-rich water in the examples 1-5 for comparison, and detecting the blood sugar content of each group of rats after use, wherein the specific steps are as follows:
1. selecting animals: strain: SD rat, sex: male, body weight: 180 g.
2. Preparing a model:
60 SD male rats with the weight of 180g are selected, and each 5 SD male rats are provided with one cage; feeding with high-fat high-sugar feed (10% of lard, 20% of sucrose, 3% of egg yolk and 67% of basal feed) at room temperature of 20-24 deg.C, feeding freely, drinking water, and increasing body weight for 30 days;
after fasting for 12 hours, rats were weighed and recorded, 2.1g of citric acid was added to 100mL of double distilled water to prepare solution A, 2.94g of sodium citrate was added to 100mL of double distilled water to prepare solution B, and A, B solutions were mixed at a ratio of 1:1.32 to determine the ph value, thereby preparing a citric acid buffer solution with ph = 4.2-4.5. Under ice bath condition, urea zotocin is dissolved in citric acid buffer solution at a concentration of 1%, and injected into the left lower abdomen of a rat at a dosage of 40.0mg/kg according to the weight of the rat on an empty stomach; continuously monitoring blood sugar after injection, wherein patients without obvious change in blood sugar value are subjected to supplementary injection once after being injected for 3 days for the first time, and patients with fasting blood sugar of more than 16.7mmol/L are selected to continue molding after being continuously monitored for 3 days after being injected for 5 days; after the molding is successful, the high-sugar and high-fat feed is continuously fed, and the high-sugar and high-fat feed is changed into a common feed after one month.
3. Grouping experiments:
after one month of molding, 52 rats had fasting blood glucose of more than 16.7mmol/L, and 48 rats were randomly selected from the rats and divided into six groups, 8 rats in the control group and 8 rats in each of examples 1 to 5.
4. Hydrogen concentration test:
the control group hydrogen concentration test adopts a dissolved water biotin concentration judgment reagent test, and the 1 st drop is not dissolved; the hydrogen concentration of the purified water is 0 ppm;
examples 1-5 Hydrogen concentration test Using a test for determining the concentration of water-soluble biotin, the test was conducted and the test was not dissolved by dropping the 9 th drop; the hydrogen concentration in example 1 was 0.6ppm, the hydrogen concentration in example 2 was 0.7ppm, the hydrogen concentration in example 3 was 0.8ppm, the hydrogen concentration in example 4 was 0.8ppm, and the hydrogen concentration in example 5 was 0.8 ppm.
5. Raising condition:
time: breeding for half a year after successful modeling;
food consumption: 50 g/piece/day;
water intake: free drinking, the control group had purified water, and examples 1 to 5 had low-frequency hydrogen-rich water.
6. General observation:
the living conditions and activities of the rats were observed daily.
7. And (4) index observation:
the blood sugar and body weight change of the rats are measured every week, and insulin tolerance and glucose tolerance experiments are carried out.
8. And (3) observing blood sugar:
diabetic rats were tested weekly for blood glucose changes.
9. Insulin, glucose tolerance test
Carrying out insulin injection to carry out an insulin resistance test, firstly measuring the blood sugar of a rat, then injecting insulin, and observing the blood sugar change conditions 50 minutes, 100 minutes, 150 minutes and 200 minutes after the insulin is injected;
glucose injection is performed to perform an anti-glucose tolerance experiment, the blood sugar of a rat is measured firstly, then glucose is injected, and the blood sugar change conditions of 50 minutes, 100 minutes, 150 minutes and 200 minutes after the glucose injection are observed.
The experimental results are as follows:
1. general observation:
experiments show that a plurality of rats in the groups of examples 1-5 move more frequently, and are obviously more active and better than the control group.
2. And (3) observing blood sugar:
the weekly timing test results show that the blood sugar of the rats in the control group is always maintained at a high level, about 30mmol/L, the average blood sugar of the rats in the examples 1-5 groups is also maintained at a high level, about 30mmol/L, 120 days before the intervention, no significant difference is seen compared with the control group, and the blood sugar of the rats is reduced, about 20mmol/L on average, and is always maintained at the level on about 120 days after the low-frequency hydrogen-rich water is drunk, and the specific results are shown in a table 1:
TABLE 1
Meanwhile, when the blood glucose of peripheral blood was measured 200 days after sacrifice of rats, the blood glucose values of the groups of examples 1 to 5 were also lower than those of the control group, as shown in table 2:
TABLE 2
| Example 1 | Example 2 | Example 3 | Example 4 | Example 5 | Control group | |
| Blood sugar value (mmol/L) | 28 | 28 | 27 | 27 | 28 | 33 |
3. Insulin testing:
the test results show that the groups of examples 1-5 are more sensitive to treatment with insulin, with significant effects and longer lasting effect than the control group, as shown in table 3:
TABLE 3
4. Glucose tolerance test:
the test results show that the blood glucose response after glucose injection was approximately the same in the examples 1-5 compared to the control group, but the fall back rate was faster and more stable, as shown in table 4:
TABLE 4
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A preparation method of low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats is characterized by comprising the following steps:
s1, adding purified water into the preparation container, sealing, and pumping out air in the water through a vacuum pump;
s2, carrying out low-frequency resonance on the purified water through a resonance instrument to obtain low-frequency water;
s3, introducing hydrogen into the low-frequency water, pressurizing by a booster pump, instantly relieving pressure to obtain low-frequency hydrogen-enriched water, and finally filling and sealing;
the mineral additive in the resonance instrument comprises the following raw materials: calcium chloride, calcium sulfate, magnesium chloride, magnesium sulfate, sodium bicarbonate, and potassium carbonate.
2. The method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats according to claim 1, wherein the method comprises the following steps: the mineral additive comprises, by weight, 2-10 parts of calcium chloride, 2-10 parts of calcium sulfate, 1-10 parts of magnesium chloride, 2-10 parts of magnesium sulfate, 6-10 parts of sodium bicarbonate and 6-10 parts of potassium carbonate.
3. The method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats according to claim 1, wherein the method comprises the following steps: and in the step S1, the vacuumizing time of the vacuum pump is 40-60 min.
4. The method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats according to claim 1, wherein the method comprises the following steps: in S2, the resonance frequency of the resonance instrument is 10-15 hz.
5. The method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats according to claim 1, wherein the method comprises the following steps: in the step S2, the low-frequency resonance time is 20-30 min.
6. The method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats according to claim 1, wherein the method comprises the following steps: and in the S3, the time for introducing the hydrogen is 20-40 min.
7. The method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats according to claim 1, wherein the method comprises the following steps: in the S3, the pressure of the booster pump is 0.1-0.5 MPa.
8. The method for preparing low-frequency hydrogen-rich water capable of reducing blood sugar of diabetic rats according to claim 1, wherein the method comprises the following steps: in S3, the filling amount of the low-frequency hydrogen-rich water is full bottle filling.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004041949A (en) * | 2002-07-12 | 2004-02-12 | Hidemitsu Hayashi | Hydrogen enriched water-forming method and hydrogen enriched water maker |
| CN104876376A (en) * | 2015-05-06 | 2015-09-02 | 成都拜尔麦迪克医疗科技有限公司 | Preparation device and preparation method of high-concentration and high-stability hydrogen-rich water |
| CN206659605U (en) * | 2016-12-17 | 2017-11-24 | *** | A kind of low-frequency resonance hydrogen-rich kettle |
| CN108408847A (en) * | 2018-03-29 | 2018-08-17 | 深圳市霍沃科技有限公司 | Misty small molecule hydrogen-rich water preparation apparatus and misty small molecule hydrogen-rich water preparation method |
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- 2022-02-22 CN CN202210159555.5A patent/CN114212945A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004041949A (en) * | 2002-07-12 | 2004-02-12 | Hidemitsu Hayashi | Hydrogen enriched water-forming method and hydrogen enriched water maker |
| CN104876376A (en) * | 2015-05-06 | 2015-09-02 | 成都拜尔麦迪克医疗科技有限公司 | Preparation device and preparation method of high-concentration and high-stability hydrogen-rich water |
| CN206659605U (en) * | 2016-12-17 | 2017-11-24 | *** | A kind of low-frequency resonance hydrogen-rich kettle |
| CN108408847A (en) * | 2018-03-29 | 2018-08-17 | 深圳市霍沃科技有限公司 | Misty small molecule hydrogen-rich water preparation apparatus and misty small molecule hydrogen-rich water preparation method |
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Application publication date: 20220322 |