CN1141976C - Deactivated clostridium perfringens disease vaccine for ox and its preparation - Google Patents
Deactivated clostridium perfringens disease vaccine for ox and its preparation Download PDFInfo
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- CN1141976C CN1141976C CNB991019431A CN99101943A CN1141976C CN 1141976 C CN1141976 C CN 1141976C CN B991019431 A CNB991019431 A CN B991019431A CN 99101943 A CN99101943 A CN 99101943A CN 1141976 C CN1141976 C CN 1141976C
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Abstract
The present invention relates to an inactivated vaccine for cattle clostridium perfringens diseases. Clostridium perfringens solutions of the types A, C and D are mixed or are respectively salted by ammonium sulfate, the salted suspended substances, namely toxoid of the clostridium perfringens solutions of the types A, C and D, are dissolved by aluminum hydroxide gel salt water, and thus, the vaccine is prepared. The inactivated vaccine has the preparation technique that the clostridium perfringens of the type A is cultured by trypsinization soup; the clostridium perfringens of the types C and D is cultured by the general technique; the toxoid which is salted by the ammonium sulfate in the clostridium perfringens solutions is dissolved by the aluminum hydroxide gel, and carries out sterilization and fungus resistance treatment. The three types of clostridium perfringens causing the cattle to be ill are prepared into the vaccine for preventing various types of bacteria which cause the cattle to suddenly die. The vaccine has good prophylactic immunization effect, and can effectively prevent the occurrence of sudden death symptoms of cattle.
Description
The present invention relates to a kind of inactivated vaccine of preventing and treating the bacillus perfringens disease, especially a kind of deactivated clostridium perfringens disease vaccine for ox and preparation technology thereof.
Bacillus perfringens＜clostridieum welchii〉be one of humans and animals important pathogenic bacteria.According to producing ectotoxic difference, this bacterium is divided into 6 bacterial types.Except that the people, bacillus perfringens mainly causes following animal epidemic: A type bacterium easily causes cattle, sheep, rabbit enterotoxemia; The Type B bacterium easily causes lamb dysentery; C type bacterium easily causes cattle, Intestinum Sus domestica toxemia, sheep struck; D type bacterium easily causes sheep, goat, calf enterotoxemia; E type bacterium easily causes sheep lamb, calf enterotoxemia.Be prevention animal bacillus perfringens disease, China have the inactivated vaccine production and supply, wherein has sheep braxy, struck, lamb dysentery, enterotoxemia to unite bacterium; The piglet red dysentery inactivated vaccine; Rabbit bacillus perfringens (A type) inactivated vaccine; The multi-joint dry powder Seedling of clostridial disease etc.Each joins bacterium and lacks the data that are used for cattle, lacks A type bacterial strain in constituting jointly.
In recent years, bacillus perfringens causes that the cattle obituary of only falling ill day by day increases, show according to data such as her Tong County of the bright city in Daxian county, Sichuan Province, Anhui Province, Tanghe County, Henan Province, Jilin Province and Lanzhou veterinary institute of the Chinese Academy of Agricultural Sciences, stable counties, Hainan Province, China recent years cattle " sudden death disease " is to be caused separately or caused with the pasteurellosis bacillus co-infection by A, C, D type bacillus perfringens.Be control cattle sudden death disease, generally join Seedling with A type bacillus perfringens or with the pasteurellosis bacillus manufacturing, do not concentrate, every cattle consumption reaches 8ml, and its shortcoming is to use inconvenience, and the disease that only single A type is caused is effective, and the disease that C, D type are caused is then invalid.
The objective of the invention is to make the various bacterium that the connection Seedling prevents to cause the cattle sudden death preparation technology of the deactivated clostridium perfringens disease vaccine for ox of the generation that its epidemic prevention is effective, can effectively prevent cattle sudden death disease for three bacillus perfringens that provide a kind of utilization to cause cattle morbidity.
Thereby another object of the present invention for provide a kind of utilize unique culture medium culturing A type clostridium toxin tire height and adopt concentration technology after join the preparation technology of Seedling, deactivated clostridium perfringens disease vaccine for ox that few, the easy to use, safe property of consumption is good.
Purpose of the present invention can realize by following measure:
A kind of deactivated clostridium perfringens disease vaccine for ox is that bacillus perfringens A type, C type, D type bacterium liquid that weight percentage is respectively 30-50%, 20-40%, 20-40% mix/are used ammonium sulfate precipitation respectively, and the weight ratio of its mixing/minute other bacterium liquid and ammonium sulfate is 100: (45-75); The 0.1-0.3 aluminium hydroxide gel saline doubly of bacillus perfringens A, the C of the toxoid of getting float after saltouing and be bacillus perfringens A, C, D type when not extracting, the long-pending total amount of D type bacteria liquid dissolves and obtains vaccine; The brinish concentration of described aluminium hydroxide gel is 18-25%.Wherein sick A, C of bacillus perfringens, D type strain are from China Veterinary Drugs Supervisory Inst., and its title code name is respectively C57-1, C59-2, C60-2.
Another object of the present invention also can realize by following measure:
A kind of preparation technology of deactivated clostridium perfringens disease vaccine for ox comprises the steps: 1. bacillus perfringens A type to be cultivated 5-12 hour results bacterium liquid with beef trypsinization soup; Bacillus perfringens C, D type are cultivated results bacterium liquid with meat liver gastric enzyme digestion soup; 2. Shou Huo bacillus perfringens A, C, D type mix/are used ammonium sulfate precipitation respectively by weight percentage 30-50%, 20-40%, 20-40% separately, and the weight ratio of its mixing/minute other bacterium liquid and ammonium sulfate is 100: (45-75); 3. the float after will saltouing is that the 0.1-0.3 aluminium hydroxide gel saline doubly of bacillus perfringens A, C when not extracting of the toxoid of bacillus perfringens A, C, D type, the long-pending total amount of D type bacteria liquid dissolves and obtains vaccine; The brinish concentration of described aluminium hydroxide gel is 18-25%; 4. add formalin by the 0.1-0.3% that dissolves the back total amount again; 5. and then vaccine is carried out aseptic process get final product.
The present invention has following advantage compared to existing technology:
1, the present invention will cause that bacillus perfringens A, C, the D type of cattle sudden death are united and join Seedling, thereby the defective that the effect that has overcome single Seedling is single, brought into play various bacterium to causing the synergy of the various disease that cattle dies suddenly, epidemic prevention of the present invention is effective, can effectively prevent the generation of cattle sudden death disease through evidence.
2, adopt improved trypsinization soup to cultivate the clostridial virulence of A type that this culture medium culturing goes out to A type bacillus perfringens among the preparation technology of the present invention and compare the high 5-10 of virulence that meat liver gastric enzyme digestion soup cultivates doubly, thereby the vaccine quality of turning out is good.
3, the present invention joins bacterium, its consumption few (every cattle is only used 2 milliliters) thereby easy to use, safe after vaccine is concentrated.
The present invention also will be described in further detail in conjunction with the embodiments:
Embodiment one:
A kind of deactivated clostridium perfringens disease vaccine for ox is weight percentage to be respectively 36%, 32%, 32% bacillus perfringens A type, C type, D type bacterium liquid mix the back ammonium sulfate precipitation, the weight ratio of bacillus perfringens A, C, D type mixed bacteria liquid and ammonium sulfate is 100: 50, and bacillus perfringens A, the C of the toxoid of getting float after saltouing and be bacillus perfringens A, C, D type when not extracting, the long-pending total amount of D type bacteria liquid 0.2 times 18% aluminium hydroxide gel saline dissolves and obtains vaccine; And then carry out aseptic process and get final product.
A kind of preparation technology of deactivated clostridium perfringens disease vaccine for ox comprises the steps: 1. bacillus perfringens A type to be cultivated 10 hours by the multi-joint dry powder seedling-growing method results of clostridial disease bacterium liquid with beef trypsinization soup; To press the multi-joint dry powder seedling-growing method preparation of clostridial disease results bacterium liquid behind bacillus perfringens C, the D type usefulness meat liver gastric enzyme digestion soup culture medium culturing; 2. Shou Huo bacillus perfringens A, C, D type are used ammonium sulfate precipitation by weight percentage 36%, 32%, 32% mixing separately, and the weight ratio of its mixed bacteria liquid and ammonium sulfate is 100: 50; 3. the float after will saltouing is that bacillus perfringens A, C when not extracting of the toxoid of bacillus perfringens A, C, D type, the long-pending total amount of D type bacteria liquid 0.2 times 18% aluminium hydroxide gel saline dissolves and obtains vaccine; 4. again by the 0.2% adding formalin of dissolving the back total amount; 5. handle and then with 37 ℃ of 1,/20,000 thimerosal that vaccine adds total amount and to get final product in 24-48 hour.Described trypsinization soup is beef to be boiled into meat soup and peptone water be made into by weight 1: 1, wherein peptone water be the beef slag that will boil with the pancreas Digestive system routinely technology digest and form.
Embodiment two:
A kind of deactivated clostridium perfringens disease vaccine for ox is weight percentage to be respectively 42%, 29%, 29% bacillus perfringens A type, C type, D type bacterium liquid use ammonium sulfate precipitation respectively, bacillus perfringens A, C, D type are 100: 60 with the weight ratio of ammonium sulfate respectively, and the toxoid of getting float after saltouing and be bacillus perfringens A, C, D type mixes bacillus perfringens A, C when not extracting, the long-pending total amount of D type bacteria liquid 0.3 times 20% aluminium hydroxide gel saline and dissolves and obtain vaccine; And then carry out aseptic process and get final product.
Its preparation technology and example together, wherein the weight percentage of the U.S. film clostridium of aerogenesis A, C, D type and with the ratio of the ratio of ammonium sulfate and aluminium hydroxide gel by requirement in the present embodiment.
Embodiment three:
A kind of deactivated clostridium perfringens disease vaccine for ox is weight percentage to be respectively 48%, 26%, 26% bacillus perfringens A type, C type, D type bacterium liquid mix the back ammonium sulfate precipitation, the weight ratio of bacillus perfringens A, C, D type mixed bacteria liquid and ammonium sulfate is 100: 72, and bacillus perfringens A, the C of the toxoid of getting float after saltouing and be bacillus perfringens A, C, D type when not extracting, the long-pending total amount of D type bacteria liquid 0.1 times 25% aluminium hydroxide gel saline dissolves and obtains vaccine; And then carry out aseptic process and get final product.
Its preparation technology and example together, wherein the weight percentage of bacillus perfringens A, C, D type and with the ratio of the ratio of ammonium sulfate and aluminium hydroxide gel by requirement in the present embodiment.
Claims (3)
1, a kind of deactivated clostridium perfringens disease vaccine for ox, it is characterized in that this vaccine is that bacillus perfringens A type, C type, D type bacterium liquid that weight percentage is respectively 30-50%, 20-40%, 20-40% mix/are used ammonium sulfate precipitation respectively, the weight ratio of its mixing/minute other bacterium liquid and ammonium sulfate is 100: (45-75); The 0.1-0.3 aluminium hydroxide gel saline doubly of bacillus perfringens A, the C of the toxoid of getting float after saltouing and be bacillus perfringens A, C, D type when not extracting, the long-pending total amount of D type bacteria liquid dissolves and obtains vaccine; The brinish concentration 18%-25% of described aluminium hydroxide gel.
2, the preparation technology of the described deactivated clostridium perfringens disease vaccine for ox of a kind of claim 1 is characterized in that comprising the steps: 1. bacillus perfringens A type being cultivated 5-12 hour results bacterium liquid with beef trypsinization soup; Bacillus perfringens C, D type are cultivated results bacterium liquid with meat liver gastric enzyme digestion soup; 2. Shou Huo bacillus perfringens A, C, D type mix/are used ammonium sulfate precipitation respectively by weight percentage 30-50%, 20-40%, 20-40% separately, and the weight ratio of its mixing/minute other bacterium liquid and ammonium sulfate is 100:(45-75); 3. be that the 0.1-0.3 aluminium hydroxide gel doubly of bacillus perfringens A, C when not extracting of the toxoid of aerogenesis pod mould clostridium A, C, D type, the long-pending total amount of D type bacteria liquid dissolves and obtains vaccine with the float behind the salt folding; The brinish concentration 18%-25% of described aluminium hydroxide gel; 4. add formalin by the 0.1-0.3% that dissolves the back total amount again; 5. and then vaccine is done to carry out aseptic process get final product.
3, the preparation technology of deactivated clostridium perfringens disease vaccine for ox as claimed in claim 2, it is characterized in that described beef trypsinization soup is beef to be boiled into meat soup and peptone water be made into by weight 1: 1, wherein peptone water be the beef slag that will boil with the pancreas Digestive system routinely technology digest and form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991019431A CN1141976C (en) | 1999-04-01 | 1999-04-01 | Deactivated clostridium perfringens disease vaccine for ox and its preparation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991019431A CN1141976C (en) | 1999-04-01 | 1999-04-01 | Deactivated clostridium perfringens disease vaccine for ox and its preparation |
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| Publication Number | Publication Date |
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| CN1269243A CN1269243A (en) | 2000-10-11 |
| CN1141976C true CN1141976C (en) | 2004-03-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB991019431A Expired - Fee Related CN1141976C (en) | 1999-04-01 | 1999-04-01 | Deactivated clostridium perfringens disease vaccine for ox and its preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102688484A (en) * | 2012-06-13 | 2012-09-26 | 北京中海生物科技有限公司 | Production method for an inactivated vaccine for type-C Clostridial enteritis |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293918C (en) * | 2003-03-13 | 2007-01-10 | 山东农业大学 | Prepn of clostridium welchil toxoid vaccine and antitoxin serum |
| CN102698259B (en) * | 2012-06-12 | 2017-02-08 | 北京中海生物科技有限公司 | Preparation method for necrotic enteritis (Type A) inactivated vaccine |
| CN103463632B (en) * | 2013-09-05 | 2016-01-06 | 山东农业大学 | The preparation method of the D type bacillus perfringens toxoid vaccine of cattle and sheep enterotoxemia |
| CN103721254A (en) * | 2013-12-31 | 2014-04-16 | 鄂尔多斯市旭和畜牧有限责任公司 | Method for preparing swine A-type clostridium perfringens aluminum hydroxide inactivated vaccine |
| CN106177935A (en) * | 2016-08-19 | 2016-12-07 | 齐鲁动物保健品有限公司 | A kind of ruminant clostridial disease tetrad inactivated vaccine and preparation method thereof |
| US20200099046A1 (en) | 2018-09-25 | 2020-03-26 | Toyota Motor Engineering & Manufacturing North America, Inc. | Nano-alloy interphase for lithium metal solid state batteries |
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1999
- 1999-04-01 CN CNB991019431A patent/CN1141976C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102688484A (en) * | 2012-06-13 | 2012-09-26 | 北京中海生物科技有限公司 | Production method for an inactivated vaccine for type-C Clostridial enteritis |
| CN102688484B (en) * | 2012-06-13 | 2016-03-30 | 北京中海生物科技有限公司 | A kind of production method of chicken necrotizing enterocolitis (C type) inactivated vaccine |
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| Publication number | Publication date |
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| CN1269243A (en) | 2000-10-11 |
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