CN114180202B - Golgi apparatus protein GP73 determination kit and chemiluminescence determination method - Google Patents
Golgi apparatus protein GP73 determination kit and chemiluminescence determination method Download PDFInfo
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- CN114180202B CN114180202B CN202111481149.2A CN202111481149A CN114180202B CN 114180202 B CN114180202 B CN 114180202B CN 202111481149 A CN202111481149 A CN 202111481149A CN 114180202 B CN114180202 B CN 114180202B
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- 102100021184 Golgi membrane protein 1 Human genes 0.000 title claims abstract description 21
- 101001040742 Homo sapiens Golgi membrane protein 1 Proteins 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title abstract description 9
- 210000002288 golgi apparatus Anatomy 0.000 title description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 22
- 238000007789 sealing Methods 0.000 claims abstract description 3
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims description 13
- 101001040734 Homo sapiens Golgi phosphoprotein 3 Proteins 0.000 claims description 13
- 238000003149 assay kit Methods 0.000 claims description 9
- 238000013016 damping Methods 0.000 claims description 5
- 238000003556 assay Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 6
- 108060003393 Granulin Proteins 0.000 abstract description 2
- 230000008093 supporting effect Effects 0.000 description 27
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 5
- 235000017491 Bambusa tulda Nutrition 0.000 description 5
- 241001330002 Bambuseae Species 0.000 description 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 5
- 239000011425 bamboo Substances 0.000 description 5
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 3
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 3
- 201000007270 liver cancer Diseases 0.000 description 3
- 208000014018 liver neoplasm Diseases 0.000 description 3
- 238000013399 early diagnosis Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/38—Devices for discharging contents
- B65D25/52—Devices for discharging successive articles or portions of contents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/28—Handles
- B65D25/2802—Handles fixed, i.e. non-swingable, handles
- B65D25/2826—Handles fixed, i.e. non-swingable, handles provided on a local area of the upper (top) wall, e.g. U-shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D43/00—Lids or covers for rigid or semi-rigid containers
- B65D43/14—Non-removable lids or covers
- B65D43/16—Non-removable lids or covers hinged for upward or downward movement
- B65D43/163—Non-removable lids or covers hinged for upward or downward movement the container and the lid being made separately
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D43/00—Lids or covers for rigid or semi-rigid containers
- B65D43/26—Mechanisms for opening or closing, e.g. pedal-operated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plasma & Fusion (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kit for measuring high-grade matrix protein GP73 and a chemiluminescent measuring method, comprising a box body and a storage barrel, wherein the box body is connected with a box cover for sealing the box body, the storage barrel is connected with a driving mechanism for driving the storage barrel to rotate, the outer side of the storage barrel is connected with a friction ring, the box cover is connected with a stop mechanism for positioning the storage barrel, the stop mechanism comprises a push-pull rod and a stop block which are oppositely arranged, the push-pull rod is used for driving the stop block to lift, the driving mechanism comprises a motor and a click button for controlling a motor switch, and the box cover is used for controlling the click button to open and close. The invention can realize the rotation of the storage cylinder, synchronously shake the reagent bottles in the storage environment, provide a good basis for the subsequent detection operation, shorten the operation time, realize the positioning of the storage cylinder, improve the stability of the storage cylinder, facilitate the medical staff to take the reagent bottles, and have flexible and convenient operation.
Description
Technical Field
The invention relates to the technical field of kits, in particular to a kit for measuring a Golgi apparatus protein GP73 and a chemiluminescent measuring method.
Background
The Golgi protein GP73 is an ideal serum marker for early diagnosis of liver cancer and postoperative recurrence condition assessment, the sensitivity and specificity are far higher than those of Alpha Fetoprotein (AFP) which is known before 40 years, and if the two are detected in a combined way, diagnosis and treatment level of the liver cancer can be greatly improved. The advent of the golgi protein GP73 was attributed to recent advances in proteomics and molecular biology. The combined detection of GP73 and AFP can improve the early diagnosis and early treatment level of liver cancer, and the kit can be used for storing the Golgi protein GP73 reagent.
When the existing kit is used, medical staff needs to take out the reagent bottle to shake and sample, and because the storage amount of the reagent bottle is more, more time is consumed to shake evenly, and the practicability is poor.
Disclosure of Invention
The invention aims to provide a kit for measuring the Golgi apparatus protein GP73 and a chemiluminescent measuring method, wherein a reagent bottle is in a storage environment and simultaneously shakes uniformly, so that a good foundation is provided for subsequent detection operation, the operation time is shortened, and the problem that more time is consumed for shaking uniformly due to more storage capacity of the reagent bottle is solved.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a high-grade matrix protein GP73 survey kit, includes box body, a storage section of thick bamboo, the box body is connected with the lid that is used for sealed box body, a storage section of thick bamboo is connected with the actuating mechanism that is used for driving a storage section of thick bamboo rotation, and is connected with the friction ring outside the storage section of thick bamboo, the lid is connected with the stop mechanism that is used for a storage section of thick bamboo location, stop mechanism includes push-pull rod, the stop block of relative setting, the push-pull rod is used for driving the lift of stop block, actuating mechanism includes motor and the click button of control motor switch, the lid is used for controlling the click button and opens and close;
when the box cover is in an open state, the click button is closed, and the stop block is connected with the friction ring;
when the box cover is in a closed state, the click button is opened, and the stop block is separated from the friction ring.
Preferably, the driving mechanism further comprises a supporting disc and a rotating shaft, the output end of the motor is connected with the rotating shaft, one end of the rotating shaft is connected to the supporting disc, the supporting disc is fixed to the bottom of the storage barrel, and the motor is fixed to the bottom end inside the box body.
Preferably, the stop mechanism further comprises a movable block, one end of the push-pull rod is connected with the box cover through a connecting shaft, the other end of the push-pull rod is connected with the movable block through a connecting shaft II, the stop block is fixed to the movable block, the movable block is slidably connected with rectangular holes formed in two sides of the box body, and the stop block penetrates through the rectangular holes.
Preferably, the height of the rectangular hole is smaller than the height of the box body.
Preferably, the bottom of the storage cylinder is connected with a supporting mechanism for supporting the storage cylinder.
Preferably, the supporting mechanism comprises at least two upright posts and at least two supporting blocks, one end of each upright post is fixed at the bottom of the storage cylinder, the other end of each upright post is connected with the supporting block, and the supporting blocks are slidably connected to the bottom end of the box body.
Preferably, the box cover is connected with the box body through a damping hinge.
Preferably, the storage barrel is provided with a storage groove for storing reagent bottles.
Preferably, the click button is connected to a switch seat fixed in the box body, the click button is electrically connected with the motor, and when the box cover is in a closed state, the box cover is pressed on the click button.
A chemiluminescent assay method of a Golgi apparatus protein GP assay kit is operated according to the following steps:
step one: the box cover is opened, the box cover is separated from the click button, the motor stops working, the storage barrel continues to rotate, and the push-pull rod pulls the movable block to rise along with the increase of the opening angle of the box cover until the stop block is connected to the friction ring, and the storage barrel stops rotating.
Step two: the reagent bottle is inserted into the containing groove of the storage cylinder.
Step three: the box cover is closed, the box cover is pressed on the click button, the stop block is separated from the friction ring at the same time, the click button starts the motor, and the motor drives the storage cylinder to rotate.
Compared with the prior art, the invention has the following beneficial effects: the invention is provided with the driving mechanism and the stopping mechanism, the driving mechanism comprises the motor, the click button, the supporting disc and the rotating shaft, the stopping mechanism comprises the push-pull rod, the stopping block and the movable block, when the box cover is in a closed state, the box cover is pressed on the click button, namely the motor drives the rotating shaft to rotate at a uniform speed, the rotation of the storage cylinder is realized, the reagent bottle is in a storage environment and simultaneously and uniformly shakes, a good foundation is provided for subsequent detection operation, and the operation time is shortened; when the box cover is in an open state, the box cover is separated from the click button, namely, the motor stops working, the push-pull rod pulls the stop block to move towards the direction close to the friction ring, along with the increase of the opening angle of the box cover, the stop block is pressed on the friction ring until the stop block is pressed, the positioning of the storage cylinder is realized, the stability of the storage cylinder is improved, the reagent bottle is convenient for medical staff to take, the operation is flexible and convenient, and the practicability is strong.
The bottom of the storage cylinder is connected with the supporting mechanism for supporting the storage cylinder, the supporting mechanism comprises at least two upright posts and at least two supporting blocks, the rotating shaft can be shared to cause pressure on the motor, meanwhile, the stability of the storage cylinder in the rotating process is guaranteed, the inclined deviation is prevented, and the use is safe and reliable.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is a left side view of the present invention;
FIG. 3 is a schematic view showing the structure of the opened state of the box cover according to the present invention;
FIG. 4 is a schematic view of a cartridge stop state configuration of the present invention;
fig. 5 is a schematic view of the structure of the cartridge of the present invention.
In the figure: 1. a box body; 2. a box cover; 3. a handle; 4. clicking a button; 5. a switch base; 6. a first connecting shaft; 7. a push-pull rod; 8. a storage cylinder; 9. a friction ring; 10. a column; 11. a support block; 12. a stopper; 13. a movable block; 14. a second connecting shaft; 15. a support plate; 16. a rotation shaft; 17. a motor; 18. a holding groove; 19. damping hinges; 20. rectangular holes.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 to 5, the invention provides a kit for determining a golgi protein GP73, which comprises a box body 1 and a storage barrel 8, wherein the box body 1 is connected with a box cover 2 for sealing the box body 1, the storage barrel 8 is connected with a driving mechanism for driving the storage barrel 8 to rotate, the outside of the storage barrel 8 is connected with a friction ring 9, the bottom of the friction ring 9 is provided with a friction surface, namely, when a stop block 12 is pressed on the friction ring 9, the storage barrel 8 can be rapidly stopped and positioned. The box cover 2 is connected with a stop mechanism for positioning the storage barrel 8, the stop mechanism comprises a push-pull rod 7 and a stop block 12 which are oppositely arranged, and the push-pull rod 7 is used for driving the stop block 12 to lift. The driving mechanism comprises a motor 17 and a click button 4 for controlling the motor 17 to switch on and off, and the box cover 2 is used for controlling the click button 4 to switch on and off.
When lid 2 is in the open mode, lid 2 and click button 4 looks separation, click button 4 closes, click button 4 top protrusion in the top of box body 1, motor 17 stops the operation, push-and-pull rod 7 pulls stop block 12 to being close to the direction of friction ring 9, along with lid 2 opening angle increase, until stop block 12 compresses tightly in friction ring 9, realize the location to storage tube 8, improve storage tube 8's stability, the medical personnel of being convenient for take the reagent bottle, the flexible operation is convenient.
When the box cover 2 is in a closed state, the click button 4 is opened, the stop block 12 is separated from the friction ring 9, the box cover 2 is pressed on the click button 4, namely, the motor 17 drives the rotary shaft 16 to rotate at a constant speed, the storage barrel 8 is rotated, the reagent bottles are synchronously shaken to be uniform while being in a storage environment, a good foundation is provided for subsequent detection operation, and the operation time is shortened.
The driving mechanism further comprises a supporting disc 15 and a rotating shaft 16, the output end of the motor 17 is connected with the rotating shaft 16, one end of the rotating shaft 16 is connected with the supporting disc 15, the supporting disc 15 is fixed at the bottom of the storage barrel 8, and the motor 17 is fixed at the bottom end inside the box body 1.
The stop mechanism further comprises a movable block 13, one end of the push-pull rod 7 is connected with the box cover 2 through a first connecting shaft 6, the other end of the push-pull rod is connected with the movable block 13 through a second connecting shaft 14, the stop block 12 is fixed on the movable block 13, the movable block 13 is connected with rectangular holes 20 arranged on two sides of the box body 1 in a sliding mode, and the stop block 12 penetrates through the rectangular holes 20. The rectangular hole 20 can be used for limiting the movable block 13, so that the movable block 13 is stably lifted, the movable stability of the stop block 12 is improved, and the height of the rectangular hole 20 is smaller than that of the box body 1.
The bottom of the storage cylinder 8 is connected with a supporting mechanism for supporting the storage cylinder 8.
The supporting mechanism comprises at least two upright posts 10 and at least two supporting blocks 11, three upright posts 10 are usually arranged, the included angle between every two adjacent upright posts 10 is 120 degrees, one end of each upright post 10 is fixed at the bottom of the corresponding storage cylinder 8, the other end of each upright post 10 is connected with each supporting block 11, and the supporting blocks 11 are connected with the bottom end of the box body 1 in a sliding mode. The supporting block 11 can share the rotation shaft 16 to cause pressure on the motor 17, meanwhile, the stability of the storage cylinder 8 in the rotation process is guaranteed, the deflection is prevented, and the use is safe and reliable.
The box cover 2 is connected with the box body 1 through the damping hinge 19, and the damping hinge 19 is convenient for positioning the box cover 2 at an angle, so that the box cover 2 is not easy to loosen at the position. The top of the box cover 2 is connected with a handle 3, and the handle 3 can facilitate the opening of the box cover 2 and the carrying of the kit.
The storage barrel 8 is provided with a storage groove 18 for storing reagent bottles, and the storage groove 18 is used for fixing the stored reagent bottles.
The click button 4 is connected to a switch seat 5 fixed in the box body 1, the click button 4 is electrically connected with a motor 17, and when the box cover 2 is in a closed state, the box cover 2 is pressed on the click button 4.
A chemiluminescent assay method of a kit for determining the Golgi apparatus protein GP73 comprises the following steps:
step one: when the box cover 2 is opened, the box cover 2 is separated from the click button 4, the motor 17 stops working, the storage barrel 8 continues to rotate, and the push-pull rod 7 pulls the movable block 13 to rise along with the increase of the opening angle of the box cover 2 until the stop block 12 is connected with the friction ring 9, and the storage barrel 8 stops rotating.
Step two: the reagent bottles are inserted into the storage grooves 18 of the storage cylinder 8.
Step three: the box cover 2 is closed, the box cover 2 is pressed on the click button 4, the stop block 12 is separated from the friction ring 9, the click button 4 starts the motor 17, and the motor 17 drives the storage cylinder 8 to rotate.
Working principle: when the kit is used, the box cover 2 is in a closed state, the box cover 2 is pressed on the click button 4, the motor 17 is in a working state, the motor 17 drives the rotary shaft 16 to rotate at a constant speed, and then the storage barrel 8 is driven to rotate, so that the reagent bottles are in a storage environment and synchronously shake uniformly, a good foundation is provided for subsequent detection operation, and the operation time is shortened. In the process of rotating the storage barrel 8, the bottom of the storage barrel 8 is connected with a stand column 10, the stand column 10 is connected with a supporting block 11 which is slidably connected to the bottom end inside the box body 1, the supporting block 11 is driven to do circular motion, a good supporting effect is provided for the storage barrel 8, the stability of the storage barrel 8 in the rotating process is guaranteed, and skew offset is prevented. The handle 3 can be held to open the box cover 2, the box cover 2 is separated from the inching button 4, namely the motor 17 stops working, the box cover 2 pulls the push-pull rod 7 to enable the movable block 13 to ascend, and then the stop block 12 is driven to move towards the direction close to the friction ring 9, along with the increase of the opening angle of the box cover 2, until the stop block 12 is pressed on the friction ring 9, the stop positioning of the storage barrel 8 is realized, the stability of the storage barrel 8 is improved, the reagent bottle in the storage groove 18 is convenient for medical staff to take, and the operation is flexible and convenient.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A kit for determining the GP73 of a golgi protein, which is characterized in that: the novel multifunctional box comprises a box body (1) and a storage barrel (8), wherein the box body (1) is connected with a box cover (2) for sealing the box body (1), the storage barrel (8) is connected with a driving mechanism for driving the storage barrel (8) to rotate, the outer side of the storage barrel (8) is connected with a friction ring (9), the bottom of the friction ring (9) is provided with a friction surface, the box cover (2) is connected with a stop mechanism for positioning the storage barrel (8), the stop mechanism comprises a push-pull rod (7) and a stop block (12) which are oppositely arranged, the push-pull rod (7) is used for driving the stop block (12) to lift, the driving mechanism comprises a motor (17) and a click button (4) for controlling the motor (17) to switch, and the box cover (2) is used for controlling the click button (4) to be closed;
the box cover (2) is connected with the box body (1) through a damping hinge (19), so that the box cover (2) is positioned at an angle, and the box cover (2) is not easy to loosen at the position;
when the box cover (2) is in an open state, the box cover (2) is separated from the click button (4), the click button (4) is closed, the push-pull rod (7) pulls the stop block (12) to move towards a direction close to the friction ring (9), along with the increase of the opening angle of the box cover (2), until the stop block (12) is pressed on the friction ring (9), the stop block (12) is connected with the friction ring (9), so that the quick stop positioning of the storage cylinder (8) is realized, and reagent bottles are conveniently taken;
when the box cover (2) is in a closed state, the click button (4) is opened, the stop block (12) is separated from the friction ring (9), the box cover (2) is tightly pressed on the click button (4), the storage barrel (8) is rotated, and the reagent bottle is synchronously and uniformly shaken while being in a storage environment.
2. The golgi protein GP73 assay kit according to claim 1, wherein the driving mechanism further comprises a support plate (15) and a rotating shaft (16), the output end of the motor (17) is connected to the rotating shaft (16), one end of the rotating shaft (16) is connected to the support plate (15), the support plate (15) is fixed to the bottom of the storage barrel (8), and the motor (17) is fixed to the bottom inside the case body (1).
3. The golgi protein GP73 assay kit according to claim 1, wherein the stopper mechanism further comprises a movable block (13), one end of the push-pull rod (7) is connected to the lid (2) through the first connecting shaft (6), the other end is connected to the movable block (13) through the second connecting shaft (14), the stopper block (12) is fixed to the movable block (13), the movable block (13) is slidably connected to rectangular holes (20) provided on both sides of the case body (1), and the stopper block (12) penetrates through the rectangular holes (20).
4. A golgi protein GP73 assay kit according to claim 3, wherein the rectangular aperture (20) has a height which is less than the height of the body (1).
5. The golgi protein GP73 assay kit according to claim 1, wherein the bottom of the cartridge (8) is connected to a support mechanism for the support of the cartridge (8).
6. The golgi protein GP73 assay kit according to claim 5, wherein the support means comprises at least two uprights (10) and at least two support blocks (11), one end of the uprights (10) being fixed to the bottom of the cartridge (8) and the other end being connected to the support blocks (11), the support blocks (11) being slidably connected to the inner bottom end of the case body (1).
7. The golgi protein GP73 assay kit according to claim 1, wherein said cartridge (8) is provided with a holding tank (18) for holding the reagent bottles.
8. The golgi protein GP73 assay kit according to claim 1, wherein the click button (4) is connected to a switch base (5) fixed in the case body (1), the click button (4) is electrically connected to the motor (17), and the case cover (2) is pressed against the click button (4) when the case cover (2) is in a closed state.
9. A chemiluminescent assay method of the golgi protein GP73 assay kit of claim 3 wherein: comprises the following steps:
step one: opening the box cover (2), separating the box cover (2) from the click button (4), stopping the motor (17) to operate, continuously rotating the storage cylinder (8), and along with the increase of the opening angle of the box cover (2), pulling the movable block (13) by the push-pull rod (7) to rise until the stop block (12) is connected with the friction ring (9), and stopping the rotation of the storage cylinder (8);
step two: inserting the reagent bottle into a containing groove (18) of a storage cylinder (8);
step three: the box cover (2) is closed, the box cover (2) is pressed on the click button (4), the stop block (12) is separated from the friction ring (9) at the same time, the click button (4) opens the motor (17), and the motor (17) drives the storage cylinder (8) to rotate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111481149.2A CN114180202B (en) | 2021-12-06 | 2021-12-06 | Golgi apparatus protein GP73 determination kit and chemiluminescence determination method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111481149.2A CN114180202B (en) | 2021-12-06 | 2021-12-06 | Golgi apparatus protein GP73 determination kit and chemiluminescence determination method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114180202A CN114180202A (en) | 2022-03-15 |
| CN114180202B true CN114180202B (en) | 2023-11-17 |
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|---|---|---|---|
| CN202111481149.2A Active CN114180202B (en) | 2021-12-06 | 2021-12-06 | Golgi apparatus protein GP73 determination kit and chemiluminescence determination method |
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| CN (1) | CN114180202B (en) |
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2021
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