CN114163268A - Protease polypeptide compound fertilizer and preparation method thereof - Google Patents
Protease polypeptide compound fertilizer and preparation method thereof Download PDFInfo
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- CN114163268A CN114163268A CN202010950240.3A CN202010950240A CN114163268A CN 114163268 A CN114163268 A CN 114163268A CN 202010950240 A CN202010950240 A CN 202010950240A CN 114163268 A CN114163268 A CN 114163268A
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- compound fertilizer
- protease polypeptide
- fertilizer
- polypeptide
- plant
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 298
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 233
- 150000001875 compounds Chemical class 0.000 title claims abstract description 225
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 223
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 223
- 108091005804 Peptidases Proteins 0.000 title claims abstract description 202
- 239000004365 Protease Substances 0.000 title claims abstract description 202
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title claims abstract description 202
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000007787 solid Substances 0.000 claims abstract description 65
- 239000012530 fluid Substances 0.000 claims abstract description 42
- 238000000034 method Methods 0.000 claims abstract description 24
- 241000196324 Embryophyta Species 0.000 claims description 80
- 239000002994 raw material Substances 0.000 claims description 67
- 238000011282 treatment Methods 0.000 claims description 36
- 108010073771 Soybean Proteins Proteins 0.000 claims description 33
- 235000010469 Glycine max Nutrition 0.000 claims description 32
- 235000019710 soybean protein Nutrition 0.000 claims description 31
- 244000068988 Glycine max Species 0.000 claims description 30
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 28
- 239000011573 trace mineral Substances 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 21
- 235000013619 trace mineral Nutrition 0.000 claims description 20
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 16
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 15
- 239000004202 carbamide Substances 0.000 claims description 15
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 14
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 14
- 229910021538 borax Inorganic materials 0.000 claims description 14
- 238000011049 filling Methods 0.000 claims description 14
- 229940099596 manganese sulfate Drugs 0.000 claims description 14
- 239000011702 manganese sulphate Substances 0.000 claims description 14
- 235000007079 manganese sulphate Nutrition 0.000 claims description 14
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 14
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 14
- 239000006012 monoammonium phosphate Substances 0.000 claims description 14
- 239000001103 potassium chloride Substances 0.000 claims description 14
- 235000011164 potassium chloride Nutrition 0.000 claims description 14
- 239000004328 sodium tetraborate Substances 0.000 claims description 14
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 14
- 239000011790 ferrous sulphate Substances 0.000 claims description 12
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 12
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 12
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 12
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 12
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 12
- 235000011151 potassium sulphates Nutrition 0.000 claims description 12
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 12
- 229960001763 zinc sulfate Drugs 0.000 claims description 12
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- 238000000465 moulding Methods 0.000 claims description 10
- 238000002844 melting Methods 0.000 claims description 9
- 230000008018 melting Effects 0.000 claims description 9
- 239000000945 filler Substances 0.000 claims description 8
- 238000005469 granulation Methods 0.000 claims description 8
- 230000003179 granulation Effects 0.000 claims description 8
- 230000002378 acidificating effect Effects 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 6
- 239000002686 phosphate fertilizer Substances 0.000 claims description 6
- 108091005658 Basic proteases Proteins 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000007888 film coating Substances 0.000 claims description 4
- 238000009501 film coating Methods 0.000 claims description 4
- 229940072033 potash Drugs 0.000 claims description 4
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 4
- 235000015320 potassium carbonate Nutrition 0.000 claims description 4
- 239000002002 slurry Substances 0.000 claims description 4
- 239000008187 granular material Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 108010064851 Plant Proteins Proteins 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 235000021118 plant-derived protein Nutrition 0.000 claims description 2
- 239000008213 purified water Substances 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 235000013877 carbamide Nutrition 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 17
- 238000003860 storage Methods 0.000 abstract description 13
- 238000003306 harvesting Methods 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 7
- 230000007774 longterm Effects 0.000 abstract description 5
- 240000008436 Ipomoea aquatica Species 0.000 description 30
- 235000001601 Sabal palmetto Nutrition 0.000 description 22
- 238000002474 experimental method Methods 0.000 description 12
- 235000015097 nutrients Nutrition 0.000 description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 235000019004 Ipomoea aquatica Nutrition 0.000 description 8
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 229910052700 potassium Inorganic materials 0.000 description 8
- 239000011591 potassium Substances 0.000 description 8
- 230000002195 synergetic effect Effects 0.000 description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 7
- 229910052698 phosphorus Inorganic materials 0.000 description 7
- 239000011574 phosphorus Substances 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 240000007124 Brassica oleracea Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 239000003797 essential amino acid Substances 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- NDLPOXTZKUMGOV-UHFFFAOYSA-N oxo(oxoferriooxy)iron hydrate Chemical compound O.O=[Fe]O[Fe]=O NDLPOXTZKUMGOV-UHFFFAOYSA-N 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229940001941 soy protein Drugs 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 239000012856 weighed raw material Substances 0.000 description 2
- 239000011787 zinc oxide Substances 0.000 description 2
- 235000011303 Brassica alboglabra Nutrition 0.000 description 1
- 235000011302 Brassica oleracea Nutrition 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000010309 melting process Methods 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 150000003112 potassium compounds Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/30—Anti-agglomerating additives; Anti-solidifying additives
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a protease polypeptide compound fertilizer and a preparation method thereof. The protease polypeptide compound fertilizer comprises a fluid compound fertilizer or a solid compound fertilizer and also comprises plant protease polypeptide, wherein the plant protease polypeptide is dispersed in the fluid compound fertilizer or the solid compound fertilizer, and the weight content of the plant protease polypeptide in the protease polypeptide compound fertilizer is 0.05-1%. The protease polypeptide compound fertilizer can effectively promote the growth of crops, improve the stress resistance of the crops, improve the quality of the crops, prolong the harvest period and the postharvest storage period of the crops and simultaneously improve the yield of the crops. And the plant protease polypeptide can also improve soil hardening and salinization caused by long-term use of the fluid compound fertilizer or the solid compound fertilizer, and improve the acid-base balance of the soil. The preparation method of the protease polypeptide compound fertilizer has simple and easily controlled process flow, and the prepared protease polypeptide compound fertilizer has stable fertilizer efficiency and is easy to realize industrialization.
Description
Technical Field
The invention belongs to the technical field of crop fertilizers, and particularly relates to a protease polypeptide compound fertilizer and a preparation method thereof.
Background
The compound fertilizer is a chemical fertilizer containing two or more nutrient elements, has the advantages of high nutrient content, few side components, good physical properties and the like, and plays an important role in balancing fertilization, improving the utilization rate of the fertilizer and promoting the high and stable yield of crops. And the compound fertilizer can be fluid compound fertilizer (liquid compound fertilizer) and solid compound fertilizer according to the form.
At present, the commonly used compound fertilizer is inorganic fertilizer, and the inorganic fertilizer is mineral fertilizer, also called chemical fertilizer, which refers to fertilizer synthesized by chemical method, including nitrogen, phosphorus and potassium compound fertilizer, and is called chemical fertilizer for short. It is characterized by simple components, high content of effective components, easy water dissolution, fast decomposition and easy absorption by root system, so it is called quick-acting fertilizer. In modern agricultural production, fertilizers are often used in large quantities to promote crop growth and yield.
However, the use of a large amount of inorganic fertilizer allows high-purity inorganic salts to be buried in the soil, and these substances are dissolved in the soil and then absorbed by plants, but have certain disadvantages:
(1) the functional requirements on the compound fertilizer at the present stage are already expanded from the stage of increasing the yield to the stage of increasing both the yield and the quality; most of fertilizers do not relate to the quality of crops and do not contribute to improving the stress resistance of the crops;
(2) although part of fertilizers solve the problem of crop quality by adding medium trace elements, the problems of low utilization rate of the trace elements and unbalanced supply are not solved, and the absorption of nutrients and the acceptance of farmers are influenced; moreover, the long-term use causes the destruction of the acid-base balance of the soil, the soil is hardened and salinized, the environment is further damaged, and the yield is reduced;
(3) at present, with the increasing marketability of vegetables, fruits and the like, long-distance transportation and long-time storage become normal states, so that the requirements on the storage period of the vegetables and the fruits are higher and higher, and most of fertilizers are not related.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a protease polypeptide compound fertilizer and a preparation method thereof, so as to overcome the technical problems that the existing compound fertilizer is not ideal for improving the quality and the stress resistance of crops, promoting the growth of the crops and prolonging the storage period of the crops.
In order to achieve the above object, according to one aspect of the present invention, there is provided a protease polypeptide complex fertilizer. The protease polypeptide compound fertilizer comprises a fluid compound fertilizer or a solid compound fertilizer and also comprises plant protease polypeptide, wherein the plant protease polypeptide is dispersed in the fluid compound fertilizer or the solid compound fertilizer or coated on the surface of the solid compound fertilizer, and the weight content of the plant protease polypeptide in the protease polypeptide compound fertilizer is 0.05-1%.
In another aspect of the invention, a preparation method of the protease polypeptide compound fertilizer is provided. The preparation method of the protease polypeptide compound fertilizer comprises the following steps:
respectively measuring the raw materials according to the components contained in the fluid compound fertilizer or the solid compound fertilizer;
mixing the measured raw materials for preparing the fluid compound fertilizer with the plant protease polypeptide; wherein the plant protease polypeptide is subjected to the mixing treatment with the raw materials according to the proportion that the plant protease polypeptide accounts for 0.05-1% of the weight of the protease polypeptide compound fertilizer;
or molding the raw materials for preparing the solid compound fertilizer and the plant protease polypeptide according to the molding method of the solid compound fertilizer; wherein the plant protease polypeptide is added according to the proportion that the plant protease polypeptide accounts for 0.05-1% of the weight of the protease polypeptide compound fertilizer.
Compared with the prior art, the protease polypeptide compound fertilizer disclosed by the invention has the advantages that the plant protease polypeptide is added into the fluid compound fertilizer or the solid compound fertilizer, and the addition amount of the plant protease polypeptide is controlled, so that the plant protease polypeptide can play a synergistic effect with crop nutrients contained in the fluid compound fertilizer or the solid compound fertilizer, the growth of crops can be effectively promoted, the stress resistance of the crops is improved, the quality of the crops is improved, the harvesting period and the storage period after harvesting can be prolonged, and meanwhile, the yield of the crops can be improved. When the protease polypeptide compound fertilizer contains the solid compound fertilizer, the protease polypeptide compound fertilizer also has the characteristic of fast disintegration, and can provide nutrients for crops relatively fast. And the plant protease polypeptide can also improve soil hardening and salinization caused by long-term use of the fluid compound fertilizer or the solid compound fertilizer, and improve the acid-base balance of the soil.
The preparation method of the protease polypeptide compound fertilizer disperses the plant protease polypeptide in the fluid compound fertilizer or the solid compound fertilizer or wraps the plant protease polypeptide on the surface of the solid compound fertilizer, so that the plant protease polypeptide can be uniformly dispersed in the fluid compound fertilizer or the solid compound fertilizer or uniformly wraps the surface of the solid compound fertilizer, and the plant protease polypeptide and the crop nutrient components contained in the fluid compound fertilizer or the solid compound fertilizer have a synergistic effect. In addition, the preparation method of the protease polypeptide compound fertilizer has simple and easily controlled process flow, and the prepared protease polypeptide compound fertilizer has stable fertilizer efficiency and is easy to realize industrialization.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In this application, the term "and/or" describes an association relationship of associated objects, meaning that there may be three relationships, e.g., a and/or B, which may mean: a is present alone, A and B are present simultaneously, and B is present alone. Wherein A and B can be singular or plural. The character "/" generally indicates that the former and latter associated objects are in an "or" relationship.
In the present application, "at least one" means one or more, "a plurality" means two or more. "at least one of the following" or similar expressions refer to any combination of these items, including any combination of the singular or plural items. For example, "at least one (a), b, or c", or "at least one (a), b, and c", may each represent: a, b, c, a-b (i.e., a and b), a-c, b-c, or a-b-c, wherein a, b, and c may be single or plural, respectively.
It should be understood that, in various embodiments of the present application, the sequence numbers of the above-mentioned processes do not mean the execution sequence, some or all of the steps may be executed in parallel or executed sequentially, and the execution sequence of each process should be determined by its function and inherent logic, and should not constitute any limitation to the implementation process of the embodiments of the present application.
The terminology used in the embodiments of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in the examples of this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.
The weight of the related components mentioned in the description of the embodiments of the present application may not only refer to the specific content of each component, but also represent the proportional relationship of the weight among the components, and therefore, the content of the related components is scaled up or down within the scope disclosed in the description of the embodiments of the present application as long as it is scaled up or down according to the description of the embodiments of the present application. Specifically, the mass in the description of the embodiments of the present application may be in units of mass known in the chemical industry, such as μ g, mg, g, and kg.
In one aspect, the embodiment of the invention provides a protease polypeptide compound fertilizer. The protease polypeptide compound fertilizer comprises a fluid compound fertilizer or a solid compound fertilizer and also comprises plant protease polypeptide, wherein the plant protease polypeptide is dispersed in the fluid compound fertilizer or the solid compound fertilizer or is coated on the surface of the solid compound fertilizer, and the weight content of the plant protease polypeptide in the protease polypeptide compound fertilizer is 0.05-1%.
The protease polypeptide compound fertilizer disclosed by the embodiment of the invention contains plant protease polypeptide, is dispersed in the fluid compound fertilizer or the solid compound fertilizer or is coated on the surface of the solid compound fertilizer, and controls the addition amount of the plant protease polypeptide, so that the plant protease polypeptide can play a role in synergism with crop nutrients contained in the fluid compound fertilizer or the solid compound fertilizer, and thus the protease polypeptide compound fertilizer disclosed by the embodiment of the invention can effectively promote the growth of crops, improve the stress resistance of the crops, improve the quality of the crops, prolong the harvesting period and the post-harvesting storage period of the crops, and simultaneously improve the yield of the crops. And due to the existence of the plant protease polypeptide, soil hardening and salinization caused by long-term use of the traditional fluid compound fertilizer or solid compound fertilizer can be improved, and the acid-base balance of soil is improved.
The fluid compound fertilizer contained in the protease polypeptide compound fertilizer of the embodiment of the invention can be a liquid compound fertilizer which is applied correspondingly according to the crop species and the soil structure characteristics, such as a liquid fertilizer containing major elements such as nitrogen, phosphorus, potassium and the like, and crop nutrient elements such as medium trace elements and the like, and can also be a liquid binary compound fertilizer. In the embodiment of the invention, the liquid ternary compound fertilizer is preferably selected, and can provide essential macro-elements and medium-trace elements for crops, so that the synergistic effect between the plant protease polypeptide and the fluid compound fertilizer is improved.
Similarly, the solid compound fertilizer contained in the protease polypeptide compound fertilizer of the embodiment of the invention can also be a liquid compound fertilizer applied correspondingly according to the crop species and the soil structure characteristics, such as a solid compound fertilizer containing major elements such as nitrogen, phosphorus, potassium and the like, and crop nutrient elements such as medium trace elements and the like, and can also be a solid binary compound fertilizer. In the embodiment of the invention, the solid ternary compound fertilizer is preferred. It can provide necessary macro-elements and medium-trace elements for crops, thereby improving the synergistic effect between the plant protease polypeptide and the fluid compound fertilizer. In an embodiment, the solid compound fertilizer is a high tower granular fertilizer, and then, the protease polypeptide compound fertilizer of the embodiment of the present invention is a protease polypeptide high tower granular compound fertilizer. At this time, in a preferred embodiment, the solid compound fertilizer is a high tower granular compound fertilizer, the plant protease polypeptide is a soy protein enzymolysis polypeptide, and the soy protein enzymolysis polypeptide coats the high tower granular compound fertilizer. Preferably, the particle size of the high tower granular compound fertilizer is 2.00-4.75 mm. At the moment, the plant protease polypeptide contained in the protease polypeptide compound fertilizer, in particular soybean protein enzymolysis polypeptide, can effectively have a synergistic effect with the solid compound fertilizer, has the characteristic of quick disintegration while endowing the synergistic effect, and quickly provides required nutrient components for crops.
The plant protease polypeptide contained in the protease polypeptide compound fertilizer of the embodiment of the invention is preferably a plant protein short peptide with 2-10 peptide chains; or preferably the plant protease polypeptide with the peptide segment with the molecular weight of less than 1000Da accounts for more than 80 percent of the plant protease polypeptide. In a more preferred embodiment, the plant protease polypeptide is a soy protease polypeptide.
By selecting and optimizing the plant protease polypeptide, the plant protease polypeptide can be improved to play a role in synergism with crop nutritional ingredients contained in the fluid compound fertilizer or the solid compound fertilizer, the growth of crops is further promoted, the stress resistance of the crops is further improved, the quality of the crops is improved, the harvesting period and the post-harvest storage period of the crops can be prolonged, and meanwhile, the yield of the crops can be improved. And the plant protease polypeptide can also improve soil hardening and salinization caused by long-term use of the fluid compound fertilizer or the solid compound fertilizer, and improve the acid-base balance of the soil. And when the polypeptide compound fertilizer is a solid compound fertilizer, the optimized plant protease polypeptide also improves the disintegration rate of the polypeptide.
Specifically, when the plant protease polypeptide is preferably soybean protein enzymolysis polypeptide, most of the plant protease polypeptide exists in the form of small molecular peptide, such as the high active peptide composed of 2-4 amino acids, can directly permeate through cell walls, directly enter cells in an original shape through the permeation of cell membranes, and is directly absorbed, utilized and converted by crops without energy consumption, so that the rapid growth of the crops is promoted. The plant protease polypeptide is preferably soybean protein enzymolysis polypeptide, is mostly in the form of small molecular peptide, can participate in the synthesis of enzyme in crops, stimulates the activity of the enzyme, strengthens the function of the enzyme, maintains the stability of the enzyme, and thus improves the stress resistance and the immunity of the crops. The plant protease polypeptide is preferably soybean protein enzymolysis polypeptide, the nutrition is rich, the soybean protease polypeptide contains a small amount of free amino acids, saccharides and inorganic salt components, the protein content of the soybean protease polypeptide is about 85%, the amino acid composition of the soybean protease polypeptide is almost completely the same as that of soybean protein, the soybean protease polypeptide usually consists of 3-6 amino acids, and the balance of essential amino acids is good and the content is rich. The plant protease polypeptide is preferably soybean protein enzymolysis polypeptide, is used as an organic matter, can organically complex trace elements to improve the quality of crops, can complex medium trace elements, enriches the nutrition of the crops, effectively conditions the growth of the crops, reduces the growth vigor and poor selling phase of the crops caused by element deficiency, supplements the trace elements, can improve the quality of the crops and enriches the mouthfeel of the crops. In addition, the soybean protein enzymolysis polypeptide can also prolong the crop harvesting period and the storage period after harvesting, the soybean protein enzymolysis polypeptide can serve as a carrier in the crops, and peptide bonds of the soybean protein enzymolysis polypeptide can strongly complex medium and trace elements such as zinc, selenium, iron and the like, so that the medium and trace elements can be directly absorbed and utilized by the crops, the utilization rate of the medium and trace elements is improved, and the quality of the crops is improved.
In one embodiment, when the plant protease polypeptide is a soybean protein enzymolysis polypeptide, the soybean protein enzymolysis polypeptide is obtained according to the following enzymolysis method:
(1): according to the following steps: 6(w/v) -1: 10(w/v) of the feed-liquid ratio, mixing the soybean protein with purified water, and preparing a soybean protein homogenate;
(2) adjusting the pH of the soybean protein homogenate to be alkaline, heating the soybean protein homogenate to 50-60 ℃, adding alkaline protease according to the proportion of 4-6% of the weight of the soybean protein for first enzymolysis treatment, then adjusting the pH to be acidic, cooling, and adding acidic protease according to the proportion for second enzymolysis treatment;
(3) and after the second enzymolysis treatment is finished, adjusting the pH value to be neutral, adding 1-2% of activated carbon for mixing treatment, sequentially centrifuging, filtering, concentrating, and then performing spray drying treatment to obtain the soybean protease polypeptide.
Wherein, the alkaline protease in the step (2) can be selected from conventional alkaline protease for protein enzymolysis, and the pH adjustment to be alkaline can be flexibly adjusted according to the optimum pH of the specifically selected alkaline protease.
Similarly, the acidic protease may be a conventional acidic protease for digesting proteins, and the adjustment of the pH to acidity may be flexibly adjusted depending on the optimum pH of the acidic protease.
The soybean protein enzymolysis polypeptide obtained by the enzymolysis method has high yield and high enzymolysis efficiency, and the obtained soybean protein enzymolysis polypeptide has low bitter taste, wherein the peptide segment ratio of 300-1000Da is the highest. Correspondingly, the soybean protease polypeptide is water-soluble soybean protease polypeptide powder, the protein content is 90-98% by mass percent, the ratio of the molecular weight below 1000Da is more than 80%, and the content of the polypeptide containing essential amino acid is rich by measurement. The soybean protease polypeptide obtained by the method has better synergistic effect with crop nutrient components contained in the fluid compound fertilizer or the solid compound fertilizer, improves soil hardening and salinization, and has better effect of improving the acid-base balance of soil.
Correspondingly, the embodiment of the invention also provides a preparation method of the protease polypeptide compound fertilizer. The preparation method of the protease polypeptide compound fertilizer comprises the following steps:
s01, respectively weighing the raw materials according to the components contained in the fluid compound fertilizer or the solid compound fertilizer;
s02, mixing the measured raw materials for preparing the fluid compound fertilizer with the plant protease polypeptide; wherein the plant protease polypeptide is subjected to the mixing treatment with the raw materials according to the proportion that the plant protease polypeptide accounts for 0.05-1% of the weight of the protease polypeptide compound fertilizer;
or molding the raw materials for preparing the solid compound fertilizer and the plant protease polypeptide according to the molding method of the solid compound fertilizer; wherein the plant protease polypeptide is added according to the proportion that the plant protease polypeptide accounts for 0.05-1% of the weight of the protease polypeptide compound fertilizer.
The fluid compound fertilizer and the solid compound fertilizer in step S01 are both the fluid compound fertilizer or the solid compound fertilizer contained in the protease polypeptide compound fertilizer, and therefore, the components contained in the fluid compound fertilizer or the solid compound fertilizer are also distributed as the components contained in the fluid compound fertilizer or the solid compound fertilizer, and then the measured amount of each raw material is also a raw material capable of preparing the fluid compound fertilizer or the solid compound fertilizer containing the components.
In the step S02, when the measured raw materials are measured according to the components contained in the fluid compound fertilizer, the measured raw materials and the plant protease polypeptide are mixed in proportion to prepare the corresponding fluid compound fertilizer according to the preparation method of the corresponding fluid compound fertilizer.
When the weighed raw materials are weighed according to the components contained in the solid compound fertilizer, the weighed raw materials and the plant protease polypeptide are mixed according to a ratio, and then the corresponding solid compound fertilizer is prepared according to the preparation method of the corresponding solid compound fertilizer. For example, when the solid compound fertilizer is a high-tower granulated compound fertilizer, the high-tower granulated compound fertilizer is prepared according to a high-tower granulated compound fertilizer preparation process, and then the high-tower granulated compound fertilizer is wrapped by the plant protease polypeptide, so that the protease polypeptide compound fertilizer is prepared. For example, in an embodiment, the solid compound fertilizer in step S01 is a high-tower granular compound fertilizer, and then the molding treatment of the raw materials and the plant protease polypeptide for preparing the solid compound fertilizer in step S02 according to the molding method of the solid compound fertilizer includes the following steps:
s021: carrying out melting treatment on a nitrogen fertilizer raw material to prepare a nitrogen fertilizer raw material melt;
s022: carrying out first mixing treatment and reaction on a phosphate fertilizer raw material, a potassium fertilizer raw material, a filling material, a medium trace element raw material and the nitrogen fertilizer raw material molten liquid to prepare molten slurry;
s023: subjecting the molten slurry to tower granulation;
s024: and (3) carrying out secondary mixing treatment on the plant protease polypeptide and the anti-hardening oil when the temperature of the fertilizer granules formed by the high-tower granulation treatment is reduced to 50-60 ℃, and then carrying out film coating treatment on a film coating machine.
Wherein, the nitrogen fertilizer raw material in step S021 may be a nitrogen fertilizer raw material commonly used in high tower granulation compound fertilizers, for example, in an embodiment, the nitrogen fertilizer raw material includes nitrogen fertilizers such as urea. The temperature of the melting process in the step S021 may be set according to the melting point temperature of the kind of the nitrogen fertilizer raw material, and if the nitrogen fertilizer raw material includes urea, the melting temperature is set to 135 ℃. The melting treatment may be performed in a high tower melting tank.
The phosphate fertilizer raw material, the potash fertilizer raw material, the filler and the medium trace element raw material in the step S022 can be selected and adjusted according to the types of crops, the soil structure and the like. In one embodiment, the phosphate fertilizer raw material comprises monoammonium phosphate, the potash fertilizer raw material comprises potassium sulfate and potassium chloride, the medium trace element raw material comprises zinc sulfate, borax, ferrous sulfate and manganese sulfate, and the filling material can be a filling material commonly used for high tower granular compound fertilizers.
The temperature for adding the plant protease polypeptide to the molten mixture in step S023 should be enough to ensure that the molten mixture has a good molten state and at the same time, the temperature of the molten mixture is 100 ℃ and 125 ℃ in one embodiment.
The ratio of the plant protease polypeptide, which in a specific embodiment is a soy protease polypeptide, to be coated onto the compound fertilizer granules in step S024 is, for example, the ratio described above. The addition amount of the anti-hardening oil can be the conventional addition amount of the high-tower granulated compound fertilizer.
Therefore, based on the types of the raw materials in steps S022, S023 and S024, in an embodiment, the urea, the monoammonium phosphate, the potassium sulfate, the potassium chloride, the zinc sulfate, the borax, the ferrous sulfate, the manganese sulfate, the soybean protease polypeptide, the anti-hardening oil and the filling material are added according to the following weight parts:
400 parts of urea 200-charge, 500 parts of monoammonium phosphate 100-charge, 600 parts of potassium sulfate 200-charge, 0-500 parts of potassium chloride, 0.5-2 parts of zinc sulfate, 1-5 parts of borax, 0.5-2 parts of ferrous sulfate, 0.1-1 part of manganese sulfate, 0.5-10 parts of soybean protease polypeptide, 1-5 parts of anti-hardening oil and 0-300 parts of filler.
Based on the material adding proportion, the urea, the monoammonium phosphate, the potassium sulfate, the potassium chloride, the zinc sulfate, the borax, the ferrous sulfate, the manganese sulfate, the soybean protease polypeptide, the anti-hardening oil and the filler can be added according to the following specific examples in parts by weight:
350 parts of urea, 170 parts of monoammonium phosphate, 320 parts of potassium sulfate and 30 parts of potassium chloride; 1 part of zinc sulfate, 2 parts of borax, 1 part of ferrous sulfate and 1 part of manganese sulfate; 10 parts of soybean protease polypeptide; 2 parts of anti-hardening oil; 120 parts of filler. Or
250 parts of urea, 310 parts of monoammonium phosphate, 270 parts of potassium sulfate and 30 parts of potassium chloride; 1 part of zinc sulfate, 2 parts of borax, 1 part of ferrous sulfate and 1 part of manganese sulfate; 5 parts of soybean protease polypeptide; 2 parts of anti-hardening oil; 130 parts of a filling material. Or
250 parts of urea, 310 parts of monoammonium phosphate and 300 parts of potassium chloride; 1 part of zinc oxide, 2 parts of borax, 1 part of ferrous sulfate and 1 part of manganese sulfate; 0.5 part of soybean protease polypeptide; 2 parts of anti-hardening oil; 130 parts of a filling material.
The granulation process in step S023 may be performed by using a granulator, for example, in an embodiment, the mixed molten mixed solution is automatically flowed into the granulator arranged at the top of the tower for granulation, the granulator discharges the material to freely fall and spray to the bottom of the tower, the material is discharged after oil cooling, and the finished product with the particle diameter of 2.00-4.75mm is obtained by sieving.
Therefore, in the preparation method of the protease polypeptide compound fertilizer in each embodiment, the plant protease polypeptide is dispersed on the surface of the fluid compound fertilizer or the solid compound fertilizer, so that the plant protease polypeptide can be uniformly dispersed on the surface of the fluid compound fertilizer or the solid compound fertilizer, and the plant protease polypeptide and the crop nutrient components contained in the fluid compound fertilizer or the solid compound fertilizer have a synergistic effect. In addition, the preparation method of the protease polypeptide compound fertilizer has simple and easily controlled process flow, and the prepared protease polypeptide compound fertilizer has stable fertilizer efficiency and is easy to realize industrialization.
The present invention will now be described in further detail by taking specific protease polypeptide compound fertilizers and methods for preparing the same as examples.
Example 1
The embodiment provides a protease polypeptide compound fertilizer and a preparation method thereof. The protease polypeptide compound fertilizer comprises a high tower granular compound fertilizer, soybean protein enzymolysis polypeptide is also dispersed in the high tower granular compound fertilizer, and the weight content of the soybean protease polypeptide in the protease polypeptide compound fertilizer is 1%.
The protease polypeptide compound fertilizer is prepared by the following methods respectively:
s1: adding a nitrogenous fertilizer raw material into a high tower melting tank for melting treatment, wherein the melting treatment temperature is 135 ℃;
s2: pumping the melted nitrogenous fertilizer raw materials into a reaction tank at the top of the tower by a pump, mixing and stirring the melted nitrogenous fertilizer raw materials with the metered phosphate fertilizer raw materials, the potassium fertilizer raw materials, the fillers and the medium trace elements lifted into the reaction tank for reaction, wherein the reaction temperature is 100-125 ℃;
s3: after the reaction is finished, automatically flowing into a granulator arranged at the top of the tower for granulation: the granulator discharges materials, freely falls and sprays the materials to the bottom of the tower, discharges the materials after oil cooling, and screens the materials to obtain a finished product with the particle diameter of 2.00-4.75 mm;
s4: and (3) carrying out secondary mixing treatment on the plant protease polypeptide and the anti-hardening oil when the temperature of the fertilizer particles is reduced to 50-60 ℃, and then coating on a coating machine.
Wherein the nitrogenous fertilizer raw material of the step S1, the phosphorus fertilizer raw material and the potassium fertilizer raw material of the step S2, the filling material, the medium trace element raw material, the soybean protein enzymolysis polypeptide and the anti-hardening oil are added according to the following proportion:
350 parts of urea, 170 parts of monoammonium phosphate, 320 parts of potassium sulfate and 30 parts of potassium chloride; 1 part of zinc sulfate, 2 parts of borax, 1 part of ferrous sulfate and 1 part of manganese sulfate; 10 parts of soybean protease polypeptide; 2 parts of anti-hardening oil; 120 parts of filler.
Example 2
The embodiment provides a protease polypeptide compound fertilizer and a preparation method thereof. The protease polypeptide compound fertilizer comprises a high tower granular compound fertilizer, soybean protease polypeptide is also dispersed in the high tower granular compound fertilizer, and the weight content of the soybean protease polypeptide in the protease polypeptide compound fertilizer is 0.5%.
The protease polypeptide compound fertilizer is prepared by the following methods respectively:
s1: refer to step S1 in example 1;
s2: refer to step S2 in example 1;
s3: refer to step S3 in example 1;
s4: refer to step S4 in embodiment 1.
Wherein the nitrogenous fertilizer raw material of the step S1, the phosphorus fertilizer raw material and the potassium fertilizer raw material of the step S2, the filling material, the medium trace element raw material, the soybean protease polypeptide and the anti-hardening oil are added according to the following proportion:
250 parts of urea, 310 parts of monoammonium phosphate, 270 parts of potassium sulfate and 30 parts of potassium chloride; 1 part of zinc sulfate, 2 parts of borax, 1 part of ferric oxide and 1 part of manganese sulfate; 5 parts of soybean protease polypeptide; 2 parts of anti-hardening oil; 130 parts of a filling material.
Example 3
The embodiment provides a protease polypeptide compound fertilizer and a preparation method thereof. The protease polypeptide compound fertilizer comprises a high tower granular compound fertilizer, soybean protease polypeptide is also dispersed in the high tower granular compound fertilizer, and the weight content of the soybean protease polypeptide in the protease polypeptide compound fertilizer is 0.05%.
The protease polypeptide compound fertilizer is prepared by the following methods respectively:
s1: refer to step S1 in example 1;
s2: refer to step S2 in example 1;
s3: refer to step S3 in example 1;
s4: reference is made to step S4 in example 1
Wherein the nitrogenous fertilizer raw material of the step S1, the phosphorus fertilizer raw material and the potassium fertilizer raw material of the step S2, the filling material, the medium trace element raw material, the soybean protease polypeptide and the anti-hardening oil are added according to the following proportion:
250 parts of urea, 310 parts of monoammonium phosphate and 300 parts of potassium chloride; 1 part of zinc oxide, 2 parts of borax, 1 part of ferrous sulfate and 1 part of manganese sulfate; 0.55 part of soybean protease polypeptide; 2 parts of anti-hardening oil; 30 parts of a filling material.
Example 4
The embodiment provides a protease polypeptide compound fertilizer and a preparation method thereof. The protease polypeptide compound fertilizer comprises a high tower granular compound fertilizer, soybean protease polypeptide is also dispersed in the high tower granular compound fertilizer, and the weight content of the soybean protease polypeptide in the protease polypeptide compound fertilizer is 0.1%.
The protease polypeptide compound fertilizer is prepared by the following methods respectively:
s1: refer to step S1 in example 1;
s2: refer to step S2 in example 1;
s3: refer to step S3 in example 1;
s4: refer to step S4 in embodiment 1.
Wherein the nitrogenous fertilizer raw material of the step S1, the phosphorus fertilizer raw material and the potassium fertilizer raw material of the step S2, the filling material, the medium trace element raw material, the soybean protease polypeptide and the anti-hardening oil are added according to the following proportion:
250 parts of urea, 310 parts of monoammonium phosphate, 270 parts of potassium sulfate and 30 parts of potassium chloride; 1 part of zinc sulfate, 2 parts of borax, 1 part of ferric oxide and 1 part of manganese sulfate; 1 part of soybean protease polypeptide; 2 parts of anti-hardening oil; 130 parts of a filling material.
Polypeptide compound fertilizer related performance test and fertilization experiment
1 protease polypeptide compound fertilizer disintegration experiment
The protease polypeptide compound fertilizer provided in the above embodiments 1 to 4 is subjected to a disintegration test according to a granular fertilizer disintegration experiment in the industry, wherein the protease polypeptide compound fertilizer provided in the embodiments 1 to 4 is respectively set as a first experiment group, a second experiment group, a third experiment group, and a fourth experiment group. The corresponding compound fertilizer without protease polypeptide is used as a control group. The results of the disintegration experiments are shown in table 1.
TABLE 1 comparison of fertilizer disintegration times
As can be seen from Table 1, the product effect of the experimental group is best, the on-duty disintegration time of the fertilizer is shortened by 13.01 percent compared with that of the control group on average, and the strength is improved by 19.20 percent.
2. Experiment for influence of protease polypeptide compound fertilizer application on crop growth vigor
The protease polypeptide compound fertilizers provided in the above examples 1 to 3 were respectively set as one experimental group, two experimental groups, and three experimental groups, and the compound fertilizer produced by the component without protease polypeptide added in the formulation of example 2 was used as a control group, and no fertilizer was applied as a blank group.
The fertilizer of each experimental group was applied to swamp cabbage under the same addition, and swamp cabbage of each experimental group was tested after the same number of days as indicated in table 2, and the test results are shown in table 2 below:
TABLE 2 growth vigor of water spinach treated differently
As can be seen from Table 2, the swamp cabbage treated with the fertilizer of the example of the present invention showed the best growth, and the stem thickness was increased by 43.04% and 15.67% respectively compared to the swamp cabbage treated with no fertilizer and the swamp cabbage treated with the fertilizer of the comparative example; the weight of each plant is increased by 34.03 percent and 16.94 percent respectively compared with the weight of the water spinach subjected to the fertilization treatment and the fertilizer application of the comparative example; the root weight of the water spinach is increased by 57.29 percent and 31.33 percent respectively compared with the water spinach subjected to no fertilization treatment and the water spinach subjected to the control fertilizer application; the root length of the swamp cabbage is increased by 32.66 percent and 17.32 percent respectively compared with the swamp cabbage which is not fertilized and is fertilized with the fertilizer of a comparative example.
3. Experiment for influence of protease polypeptide compound fertilizer application on crop stress resistance
The protease polypeptide compound fertilizers provided in the above examples 1 to 3 were respectively set as one experimental group, two experimental groups, and three experimental groups, and the compound fertilizer produced by the component without protease polypeptide added in the formulation of example 2 was used as a control group, and no fertilizer was applied as a blank group.
The fertilizer of each experimental group is applied to the water spinach under the same addition, and the water spinach of each group is cultivated under the following stress resistance:
and selecting plants with uniform and robust growth vigor in each treatment in a vigorous period, putting the plants into an incubator with the temperature of 4 ℃ for low-temperature treatment for 48 hours, then recovering the normal temperature, sampling the leaves of each treated plant and measuring the relative conductivity of the leaves.
The test results are shown in table 3 below:
TABLE 3 relative conductivity of different treatments to leaf of Brassica Oleracea
| Experimental group | Relative electrical conductivity of the blades |
| Does not need to be fertilized | 0.18±0.02 |
| Comparative example | 0.17±0.03 |
| Example 1 | 0.12±0.01 |
| Example 2 | 0.14±0.05 |
| Example 3 | 0.12±0.02 |
As can be seen from Table 3, the leaf relative conductivity of the swamp cabbage treated with the fertilizer of the example was the lowest, and the leaf relative conductivity was reduced by 29.63% and 25.49% respectively compared to the swamp cabbage treated with no fertilizer and the swamp cabbage treated with the fertilizer of the comparative example. The fertilizer of the embodiment has the obvious effect of improving the stress resistance of crops.
4. Experiment for influence of protease polypeptide compound fertilizer application on crop quality
The protease polypeptide compound fertilizers provided in the above examples 1 to 3 were respectively set as one experimental group, two experimental groups, and three experimental groups, and the compound fertilizer produced by the component without protease polypeptide added in the formulation of example 2 was used as a control group, and no fertilizer was applied as a blank group.
The fertilizer of each experimental group was applied to swamp cabbage under the same addition, and the quality index in table 4 was tested after the same number of days for swamp cabbage of each experimental group, and the test results are shown in table 4 below:
TABLE 4 Effect of different treatments on the quality of the cabbage
As can be seen from Table 4, the swamp cabbage treated with the fertilizer of the example had the best quality, and the protein content thereof was increased by 19.08% and 13.60% respectively as compared with the swamp cabbage treated with no fertilizer and the swamp cabbage treated with the fertilizer of the comparative example; the soluble sugar content was increased by 18.34% and 15.31% respectively compared to the swamp cabbage treated with no fertilizer and with the control fertilizer.
5. Experiment for influence of protease polypeptide compound fertilizer application on storage of picked crops
The protease polypeptide compound fertilizers provided in the above examples 1 to 3 were respectively set as one experimental group, two experimental groups, and three experimental groups, and the compound fertilizer produced by the component without protease polypeptide added in the formulation of example 2 was used as a control group, and no fertilizer was applied as a blank group.
Swamp cabbage from each experimental group was fertilized with the same addition, picked after the same number of days and stored as in table 5, and swamp cabbage from each group was measured as indicated in table 4, with the test results shown in table 5 below:
TABLE 5 Effect of different treatments on the post-harvest storage time of swamp cabbage
As can be seen from Table 5, the postharvest storage time of the swamp cabbage treated with the fertilizer of the example is longest, and the normal-temperature storage time of the swamp cabbage treated with the fertilizer of the example is increased by 33.99% and 28.93% respectively compared with that of the swamp cabbage not treated with the fertilizer and treated with the fertilizer of the comparative example; the cold storage increases 22.87% and 19.89% respectively compared with the water spinach without fertilization treatment and with the fertilizer of the comparative example.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (9)
1. The protease polypeptide compound fertilizer comprises a fluid compound fertilizer or a solid compound fertilizer and is characterized by also comprising plant protease polypeptide, wherein the plant protease polypeptide is dispersed in the fluid compound fertilizer or the solid compound fertilizer or is coated on the surface of the solid compound fertilizer, and the weight content of the plant protease polypeptide in the protease polypeptide compound fertilizer is 0.05-1%.
2. The protease polypeptide compound fertilizer as claimed in claim 1, characterized in that: the plant protease polypeptide is a plant protein short peptide with 2-10 peptide chains; or
The peptide segment with the molecular weight of less than 1000Da in the plant protease polypeptide accounts for more than 80%.
3. The protease polypeptide complex fertilizer according to claim 1 or 2, characterized in that: the plant protease polypeptide is soybean protein enzymolysis polypeptide.
4. The protease polypeptide complex fertilizer of claim 3, characterized in that: the soybean protein enzymolysis polypeptide is obtained according to the following enzymolysis method:
according to the following steps: 6(w/v) -1: 10(w/v) of the feed-liquid ratio, mixing the soybean protein with purified water, and preparing a soybean protein homogenate;
adjusting the pH of the soybean protein homogenate to be alkaline, heating the soybean protein homogenate to 50-60 ℃, adding alkaline protease according to the proportion of 4-6% of the weight of the soybean protein for first enzymolysis treatment, then adjusting the pH to be acidic, cooling, and adding acidic protease according to the proportion for second enzymolysis treatment;
and after the second enzymolysis treatment is finished, adjusting the pH value to be neutral, adding 1% -2% of activated carbon for mixing treatment, sequentially centrifuging, filtering, concentrating, and then performing spray drying treatment to obtain the soybean protein enzymolysis polypeptide.
5. The protease polypeptide complex fertilizer according to any one of claims 1-2, 4, characterized in that: the solid compound fertilizer is a high tower granular compound fertilizer, the plant protease polypeptide is soybean protein enzymolysis polypeptide, and the soybean protein enzymolysis polypeptide coats the high tower granular compound fertilizer.
6. The protease polypeptide compound fertilizer as claimed in claim 5, characterized in that: the particle size of the high tower granular compound fertilizer is 2.00-4.75 mm.
7. A preparation method of a protease polypeptide compound fertilizer comprises the following steps:
respectively measuring the raw materials according to the components contained in the fluid compound fertilizer or the solid compound fertilizer;
mixing the measured raw materials for preparing the fluid compound fertilizer with the plant protease polypeptide; wherein the plant protease polypeptide is subjected to the mixing treatment with the raw materials according to the proportion that the plant protease polypeptide accounts for 0.05-1% of the weight of the protease polypeptide compound fertilizer;
or molding the raw materials for preparing the solid compound fertilizer and the plant protease polypeptide according to the molding method of the solid compound fertilizer; wherein the plant protease polypeptide is added according to the proportion that the plant protease polypeptide accounts for 0.05-1% of the weight of the protease polypeptide compound fertilizer.
8. The method of claim 7, wherein: the solid compound fertilizer is a high-tower particle compound fertilizer, and the molding treatment of the measured raw materials for preparing the solid compound fertilizer and the plant protease polypeptide according to the molding method of the solid compound fertilizer comprises the following steps:
carrying out melting treatment on a nitrogen fertilizer raw material to prepare a nitrogen fertilizer raw material melt;
mixing and reacting a phosphate fertilizer raw material, a potash fertilizer raw material, a filler, a medium trace element raw material and the nitrogen fertilizer raw material molten liquid to prepare molten slurry, and performing high tower granulation on the molten slurry;
and (3) carrying out secondary mixing treatment on the plant protease polypeptide and the anti-hardening oil when the temperature of the fertilizer granules formed by the high-tower granulation treatment is reduced to 50-60 ℃, and then carrying out film coating treatment on a film coating machine.
9. The method of claim 8, wherein: the nitrogenous fertilizer raw material comprises urea, the phosphate fertilizer raw material comprises monoammonium phosphate, the potash fertilizer raw material comprises potassium sulfate and potassium chloride, the medium trace element raw material comprises zinc sulfate, borax, ferrous sulfate and manganese sulfate, the plant protease polypeptide is soybean protein enzymolysis polypeptide, and the urea, monoammonium phosphate, potassium sulfate, potassium chloride, zinc sulfate, borax, ferrous sulfate, manganese sulfate and soybean protease polypeptide are added with the anti-hardening oil and the filling material according to the following proportion by weight:
400 parts of urea 200-charge, 500 parts of monoammonium phosphate 100-charge, 600 parts of potassium sulfate 200-charge, 0-500 parts of potassium chloride, 0.5-2 parts of zinc sulfate, 1-5 parts of borax, 0.5-2 parts of ferrous sulfate, 0.1-1 part of manganese sulfate, 0.5-10 parts of soybean protease polypeptide, 1-5 parts of anti-hardening oil and 0-300 parts of filler.
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