CN114134126A

CN114134126A - Application of transaminase and mutant thereof in preparation of (S) -1-methoxy-2-propylamine

Info

Publication number: CN114134126A
Application number: CN202111259352.5A
Authority: CN
Inventors: 杨立荣; 张涛; 周海胜; 张红玉; 吴坚平
Original assignee: ZJU Hangzhou Global Scientific and Technological Innovation Center
Current assignee: ZJU Hangzhou Global Scientific and Technological Innovation Center
Priority date: 2021-10-28
Filing date: 2021-10-28
Publication date: 2022-03-04
Anticipated expiration: 2041-10-28
Also published as: CN114134126B

Abstract

The invention discloses application of transaminase and mutants thereof in preparation of (S) -1-methoxy-2-propylamine. The transaminase with extremely high activity for preparing (S) -1-methoxy-2-propylamine from the catalytic substrate 1-methoxy-2-acetone is obtained by screening, and the transaminase is applied to the (S) -1-methoxy-2-propylamine, so that the ee value of a product obtained by the reaction reaches over 99 percent. The invention also mutates the wild transaminase by site-directed mutagenesis technology, and the enzyme activity of the screened mutant is further improved, and the conversion rate is obviously improved. The method takes 1-methoxy-2-acetone as a substrate, and the chiral pure (S) -1-methoxy-2-propylamine is directly prepared by the amido transfer reaction of the substrate catalyzed by omega-transaminase in the presence of isopropylamine or isopropylamine salt and cofactors.

Description

Application of transaminase and mutant thereof in preparation of (S) -1-methoxy-2-propylamine

Technical Field

The invention relates to the technical field of biochemical engineering, and particularly relates to application of wild transaminase in preparation of (S) -1-methoxy-2-propylamine, a transaminase mutant and application of the transaminase mutant in preparation of (S) -1-methoxy-2-propylamine.

Background

(S) -1-methoxy-2-propylamine is a key chiral intermediate in the synthesis of the chloroacetamide herbicides, Metolachlor (Metholachlor) and Dimethenamid (Dimethenamide) (FIG. 1). Metolachlor was discovered in 1970 by Ciba-Geigy, Inc. (now Daidan), and was marketed in 1975. S-isomer isolated from S-metolachlor has higher activity, lower toxicity and better safety, and is first marketed in the united states in 1997. Metolachlor is the largest variety in amide herbicides, the global sale amount in 2018 is 6.45 billion dollars, the metolachlor occupies the sixth place of the global herbicide market, and the compound annual growth rate in 2013 and 2018 is 0.9 percent. Metolachlor is widely applied to crops such as corn, sunflower, soybean, cotton, beet, sugarcane and the like. In recent years, the high-efficiency isomer and the compound product thereof are continuously developed and brought into the market, and the market sale of the high-efficiency isomer and the compound product thereof is further promoted. In 2018, the Chengda company applies for registering a mesotrione and s-metolachlor compound agent for preventing and removing annual gramineous weeds in a corn field to portuga and Spain; the same year company, dicamba + s-metolachlor combination, Tavium Plus Vapor Grip Technology, obtained us registration for use at the early stage of emergence before planting glyphosate resistant soybeans. In 2019, a compound preparation of pionorflurtamone, mesotrione and s-metolachlor (Acuron Flexi) is marketed in Canada and is used for preventing and removing some important weeds in corn fields, such as Chenopodium album (Chenopodium album), Amaranthus retroflexus (Amaranthus retroflexus) and the like, and annual gramineous weeds. Dimethenamid was marketed by basf in 1993, with a global sales of $ 2.19 billion in 2018 and a compound annual growth rate of 6.0% in 2013-2018. The dimethenamid can be used for preventing and killing annual gramineous weeds and part of broadleaf weeds in corn, rape, soybean, corn, sunflower and the like, and is an important herbicide for corn. The market benefits from a plurality of compound products and high-efficiency isomers. In 2019, the dimethenamid obtained the registration of European union renewal in 15 years, and the validity period was 9 months from 2034.

The synthesis of (S) -1-methoxy-2-propylamine with high optical purity is crucial to the production and preparation of the two herbicides. Chinese patent application publication No. CN110066223A discloses a chemical method for preparing 1-methoxy-2-propylamine: the 1-methoxy-2-acetone is reduced and aminated into 1-methoxy-2-propylamine by adopting a metal catalyst under the conditions of high temperature and high pressure. The method has the advantages of expensive catalyst, harsh reaction conditions, low product yield and lack of optical purity data, and does not accord with the trend of green development of pesticide chemical industry, so the method is not suitable for large-scale industrialization.

Chinese patent application publication No. CN1292828 discloses a method for preparing (S) -1-methoxy-2-propylamine by using transaminase to catalyze 1-methoxy-2-acetone, and the method has the advantages of mild reaction conditions and high optical purity of the product. However, the lack of a gene source for the transaminase, a key catalyst, and a process for preparing the transaminase, limit further large-scale application of the process.

Disclosure of Invention

The invention provides application of wild transaminase in preparation of (S) -1-methoxy-2-propylamine, transaminase mutants and application of the transaminase mutants in preparation of (S) -1-methoxy-2-propylamine, aiming at the defects of the existing (S) -1-methoxy-2-propylamine synthesis process.

The specific technical scheme is as follows:

the invention provides application of transaminase in preparation of (S) -1-methoxy-2-propylamine, wherein the amino acid sequence of the transaminase is shown as SEQ ID No. 2.

Further, the nucleotide sequence of the gene coding the transaminase is shown as SEQ ID NO. 1.

In order to obtain the transaminase, the invention constructs a omega-transaminase enzyme library, and the enzyme library is constructed by the following steps: (1) collecting the source of omega-transaminase and related information of gene sequences through literature research and multi-sequence comparison; (2) querying omega-transaminase gene sequences or amino acid sequences from different sources through a gene database (https:// www.ncbi.nlm.nih.gov/genome /) to carry out whole gene synthesis; (3) integrating the synthesized gene fragment into an expression plasmid; (4) transforming an expression plasmid comprising a further ω -transaminase gene into an expression host bacterial cell; (5) sequencing to verify whether the recombinant bacteria are successfully constructed; (6) successfully constructing recombinant bacteria, numbering, and storing in a refrigerator at the temperature of minus 80 ℃ for later use.

The transaminase is a wild-type transaminase derived from Bacillus (Bacillus sp.) Soil768D1, and has NCBI accession number KRF 52528.1.

The invention provides application of genetically engineered bacteria in preparation of (S) -1-methoxy-2-propylamine, wherein the genetically engineered bacteria comprise transaminase genes with coding nucleotide sequences shown as SEQ ID No. 1.

The invention also provides a transaminase mutant, which is one of the following:

(1) mutating 303 th serine of amino acid shown in SEQ ID NO.2 into glycine;

(2) mutating phenylalanine at the 381 th position of the amino acid shown in SEQ ID NO.2 into valine;

(3) the 382 nd aspartic acid of the amino acid shown in SEQ ID NO.2 is mutated into arginine;

(4) the 391 th aspartic acid of the amino acid shown in SEQ ID NO.2 is mutated into glutamic acid;

(5) the 392 nd isoleucine of the amino acid shown in SEQ ID NO.2 is mutated into leucine.

The invention also provides a coding gene of the transaminase mutant.

The invention also provides a recombinant vector containing the coding gene; the recombinant vector comprises a pET series vector, a shuttle vector, a bacteriophage or a virus vector; more preferably, the expression vector is pET-28a (+).

The invention also provides a genetic engineering bacterium containing the coding gene; the host cell can be prokaryotic cell, yeast or eukaryotic cell; more preferably, the host cell is E.coli BL21(DE 3).

The invention also provides application of the transaminase mutant or the genetically engineered bacterium in preparation of (S) -1-methoxy-2-propylamine.

The present invention also provides a process for the preparation of (S) -1-methoxy-2-propylamine which comprises: taking 1-methoxy-2-acetone as a substrate, and carrying out non-para-amidogen transfer reaction by using a catalyst in the presence of isopropylamine or isopropylamine salt and a cofactor to obtain (S) -1-methoxy-2-propylamine, wherein the reaction formula is shown in figure 2.

In the reaction system, the omega-transaminase used as the catalyst is crude enzyme liquid after cell disruption, engineering bacteria resting cells for expressing recombinant omega-transaminase, purified pure enzyme or immobilized enzyme. Therefore, the catalyst is wild transaminase with an amino acid sequence shown as SEQ ID NO.2 or immobilized enzyme thereof, or transaminase mutant or immobilized enzyme thereof as described above, or genetically engineered bacteria as described above.

Further, the cofactor is pyridoxal phosphate, or pyridoxamine phosphate, or pyridoxine phosphate; preferably, the amount of the cofactor added is 0.1 to 100 mM.

Furthermore, in the reaction system, the adding amount of the catalyst is calculated by the wet weight of the cells after being centrifuged at 4000rpm for 30min, and the adding amount of the cells is 0.5-15% of the weight of the reaction solution. The concentration of the substrate 1-methoxy-2-acetone is 0.1-2.0M. The addition amount of the amino donor isopropylamine or isopropylamine salt is 0.15-10.0M.

Further, the temperature of the non-paragenic amido transfer reaction is 10-70 ℃, the time is 6-72 hours, and the pH value of the reaction liquid is 6-10. More preferably, the temperature is 20-60 ℃ and the time is 12-36 h.

Compared with the prior art, the invention has the following beneficial effects:

(1) the transaminase with extremely high activity for preparing (S) -1-methoxy-2-propylamine from the catalytic substrate 1-methoxy-2-acetone is obtained by screening, and the transaminase is applied to the (S) -1-methoxy-2-propylamine, so that the ee value of a product obtained by the reaction reaches over 99 percent.

(2) The invention also mutates the wild transaminase by site-directed mutagenesis technology, and the enzyme activity of the screened mutant is further improved, and the conversion rate is obviously improved.

(3) The method takes 1-methoxy-2-acetone as a substrate, and the chiral pure (S) -1-methoxy-2-propylamine is directly prepared by the amido transfer reaction of the substrate catalyzed by omega-transaminase in the presence of isopropylamine or isopropylamine salt and cofactors.

Drawings

FIG. 1 shows the molecular structures of (S) -1-methoxy-2-propylamine, metolachlor and dimethenamid.

FIG. 2 shows the reaction scheme of ω -transaminase catalyzed 1-methoxy-2-propanone to prepare (S) -1-methoxy-2-propylamine.

FIG. 3 is HPLC detection spectrum of 1-methoxy-2-propylamine: wherein the retention time is 25.432min is (S) -1-methoxy-2-propylamine, and 27.212min is (R) -1-methoxy-2-propylamine.

FIG. 4 is a QC detection spectrum of the reaction process for preparing (S) -1-methoxy-2-propylamine from 1-methoxy-2-acetone catalyzed by ω -transaminase, in which: the retention time is 2.232min for isopropylamine, 2.340min for acetone, 2.740min for ethyl acetate solvent, 3.982min for 1-methoxy-2-propylamine, 4.648min for internal standard n-nonane, and 5.482min for 1-methoxy-2-acetone.

Detailed Description

The experimental methods in the present invention are conventional methods unless otherwise specified, and the gene cloning procedures can be specifically described in molecular cloning protocols, compiled by J. Sambruka et al. The invention relates to a recombinant Escherichia coli BL21(DE3) with omega-transaminase gene, and pET-28a (+) is purchased from TAKARA. Reagents used in the downstream catalytic process: 1-methoxy-2-propanone, (S) -1-methoxy-2-propylamine, and (R) -1-methoxy-2-propylamine. From Aladdin Chemicals, Inc.; other commonly used reagents are available from the national pharmaceutical group chemical agents, ltd. The three-letter or one-letter expression of amino acids used in the present application uses the amino acid code specified by IUPAC (Eur. J. biochem.,138:9-37,1984).

The optical purity of the product is detected by adopting High Performance Liquid Chromatography (HPLC), and the specific method comprises the following steps: 1) chromatographic conditions are as follows: the type of the chromatographic column:

QS-C18,5 μm,4.6 mm. times.250 mm. Mobile phase: 50mM sodium acetate solution acetonitrile 8: 2. Detection wavelength: 338 nm. Flow rate: 1.0 mL/min. Column temperature: at 30 ℃. 2) Derivatization reagent: 0.03g of o-phthalaldehyde and 0.1N-acetyl-L-cysteine are respectively weighed, dissolved with 400 mu L of ethanol, and then 4mL of 0.1M boric acid-sodium hydroxide buffer solution (Na is weighed)₂B₄O₇·10H₂O15.25 g and NaOH 0.66g, to a volume of 200mL, dissolved by sonication at pH 10.0, shaken to dissolve them thoroughly, and kept in a refrigerator at 4 ℃ for further use (no more than 4 days). 3) Derivatization reaction and determination: 100 mul of sample is taken and added with 100 mul of derivatization reagent, the mixture is evenly mixed and then is kept at 25 ℃ for 5min, and 20 mul of sample injection is carried out for analysis.

Detecting the conversion of each substance in the reaction process by adopting gas chromatography (QC), wherein the specific method comprises the following steps: the type of the chromatographic column: GT-a capillary column, detector temperature: 240 ℃, injector temperature: 240 ℃, column furnace temperature: at 70 ℃.

Example 1 construction and Activity measurement of Gene engineering bacteria expressing wild type omega-transaminase

Acquisition of the Mono, omega-transaminase Gene

Collecting the source of omega-transaminase and related information of gene sequences through literature research and multi-sequence comparison; querying omega-transaminase gene sequences or amino acid sequences from different sources through a gene database (https:// www.ncbi.nlm.nih.gov/genome /) to carry out whole gene synthesis; specific information on the obtained nitrile hydratase is shown in Table 1.

TABLE 1 information on the ω -transaminase enzyme library

II, construction of a Strain expressing a wild-type omega-transaminase

Submitting the omega-aminotransferase gene sequence to a gene synthesis company for total gene synthesis, and constructing on a plasmid vector pET-28a (+), wherein the restriction enzyme sites are EcoRI/BamHI and HindIII; and then introducing the constructed plasmid into an expression host E.coli BL21(DE3) strain, namely the genetic engineering strain E.coli BL21(DE3)/pET-28a (+) -TAx.

Recombinant expression of wild-type omega-aminotransferase

Inoculating the successfully constructed engineering bacteria into an LB liquid culture medium, shaking the engineering bacteria in a shaking table at 37 ℃, carrying out shake culture at 200rpm for 2-3 h, cooling the engineering bacteria to 18 ℃ when the density OD600 value of the bacteria reaches 0.8, and adding IPTG until the final concentration is 0.5 mM. The flask was then transferred to a shaker at 18 ℃ and incubated for a further 16h at 200 rpm. After the culture is finished, the culture solution is centrifuged for 30min at 4000rpm, the supernatant is discarded, the somatic cells are collected, then the somatic cells are resuspended by 100mM phosphate buffer solution with the pH of 8.0, the bacterial suspension is ultrasonically crushed, and the precipitate is removed by centrifugation, so that the obtained supernatant is crude enzyme solution.

Enzyme activity determination of four, recombinant omega-aminotransferase

The enzyme activity of the recombinant omega-transaminase is detected by using a 1-methoxy-2-acetone substrate, and the determination system is as follows: the total reaction system is 1mL, and comprises 200 μ L of 500mM isopropylamine hydrochloride solution, 200 μ L of 100mM substrate solution, 100 μ L of 10mM pyridoxal phosphate (PLP) solution, and 500 μ L of crude enzyme solution for cell disruption of engineering bacteria, which are all prepared by using 100mM phosphate buffer solution with pH 8.0. The reaction was shaken at 40 ℃ for 15min and quenched by the addition of 100. mu.L of 1M NaOH. The reaction mixture was centrifuged at 12000rpm for 5min to remove cells and enzyme proteins. The (S) -1-methoxy-2-propylamine produced in the reaction system was measured by high performance liquid chromatography, and the enzyme activity was defined as follows: at 40 ℃, the enzyme amount which can convert the substrate into 1 mu mol of product in 1min is 1U. All recombinant ω -transaminases were subjected to the activity assay, and the results are shown in table 4. As can be seen from Table 4, the activity of the omega-transaminase derived from Bacillus sp.Soil768D1 is the highest and is nearly two orders of magnitude higher than that of other enzymes.

TABLE 2 determination of enzyme activity and optical selectivity of omega-transaminases

Example 2 construction and Activity measurement of genetically engineered bacteria expressing mutant omega-transaminase

Acquisition of mono, omega-transaminase template genes

A glycerol tube storing the recombinant bacterium E.coli BL21(DE3)/pET-28a (+) -TA15 was streaked onto a dish containing LB solid medium (50. mu.g/mL kanamycin), and was cultured at 37 ℃ for 12 hours. A single colony was picked from the plate, inoculated into 5mL of LB medium containing 50. mu.g/mL of kanamycin, and cultured at 37 ℃ and 200rpm for 12 hours. After obtaining the culture solution, extraction of plasmids was performed according to the instructions of the plasmid extraction kit.

Site-directed mutagenesis of di-, omega-aminotransferase genes

And (3) introducing site-directed mutation into the TA15 gene by using the pET-28a (+) -TA15 plasmid extracted in the step one as a template through whole-plasmid PCR, wherein the used primers and a PCR reaction system are respectively shown in tables 1 and 2.

TABLE 1 primers used for PCR

TABLE 2 PCR amplification System

Components	Volume (μ L)
		PrimeSTAR	20
Upstream primer	0.8
		Downstream primer	0.8
Plasmid template	0.4
		ddH₂O	18

The pET-28a (+) -TA15 plasmid was used as a template, point mutation PCR was performed using the primers shown in Table 1, the PCR amplification system is shown in Table 2, and the PCR amplification conditions were as follows:

1) pre-denaturation: 5min at 98 ℃;

2) denaturation: 10s at 98 ℃; annealing: 15s at 60 ℃; extension: 1min at 72 ℃ for 30 s; circulating for 35 times;

3) extension: 10min at 72 ℃;

4) storing at 4 ℃ for 2.0 h.

After PCR amplification, the amplified product was detected by 1.0% agarose gel electrophoresis, and the target band was recovered by purification using a DNA recovery and purification kit. The recovered PCR product was digested with DPN I and the template was removed. The digestion system is shown in table 3.

TABLE 3 digestion System

Reagent	Volume (μ L)
		PCR amplification product/plasmid	8.5
DPNI	0.5
		10×Buffer	1

Digestion conditions are as follows:

1)37℃：1h；

2)75℃：15min；

3) storing at 4 ℃ for 2.0 h.

Construction of mutant omega-transaminase-expressing strains

The digested product is transferred into competent cells of Escherichia coli BL21(DE3), then sequenced by Beijing Optimalaceae New Biotechnology Limited company, and plasmids with correct sequencing result are expressed to obtain recombinant strains.

Performing inducible expression (the operation is the same as the third step in the example 1), and finally obtaining nine single-point mutated transaminase mutants, namely TA15-S244G, TA15-T259C, TA15-S303G, TA15-A307L, TA15-V341A, TA15-F381V, TA15-D382R, TA15-D391E and TA 15-I392L; the wild type was designated TA 15-WT.

Enzyme activity and thermal stability determination of mutant omega-transaminase

The method and the procedure for measuring the enzyme activity were as described in the fourth step of example 1.

Thermostability characterization of the enzymes: and (3) respectively placing the enzyme solutions in hot water bath at 40 ℃ for treatment for 60min, and then determining residual enzyme activity by using an enzyme activity detection system, wherein the residual relative enzyme activity defines the untreated initial enzyme activity as 100%. The data of the enzyme activity measurement are shown in Table 3 below.

TABLE 4 determination of the enzyme activities of wild type and mutant TA15 transaminase

Example 3

The engineered Escherichia coli expressing the ω -transaminase of TA15 obtained in example 1 was cultured by the method described in example 1 to obtain a crude enzyme solution.

The reaction system was 10mL, containing 100mM substrate 1-methoxy-2-propanone, 200mM isopropylamine, 1mM PP, and TA15 crude enzyme solution (0.05 g as wet bacteria) was added, the reaction temperature was controlled to 35 ℃ by a metal bath, and the pH during the reaction was controlled to 7.5 by 100mM phosphate buffer. Reaction for 12h, and gas chromatography detection of the generation amount of 89.8mM of (S) -1-methoxy-2-propylamine; the concentration of the raw material 1-methoxy-2-acetone is 10.1mM, and the conversion rate is 89.9%; the detection product of the high performance liquid chromatography is mainly (S) -1-methoxy-2-propylamine, and the ee value is 99.5 percent.

Example 4

The engineered Escherichia coli expressing the ω -transaminase of TA15-D382R obtained in example 1 was cultured by the method described in example 1 to obtain a crude enzyme solution.

The reaction system was 10mL, containing 100mM substrate 1-methoxy-2-propanone, 200mM isopropylamine, 1mM PP, and TA15 crude enzyme solution (0.05 g as wet bacteria) was added, the reaction temperature was controlled to 35 ℃ by a metal bath, and the pH during the reaction was controlled to 7.5 by 100mM phosphate buffer. Reacting for 12h, and detecting the generation amount of 90.3mM of (S) -1-methoxy-2-propylamine by gas chromatography; the concentration of the raw material 1-methoxy-2-acetone is 8.7mM, and the conversion rate is 91.3%; the detection product of the high performance liquid chromatography is mainly (S) -1-methoxy-2-propylamine, and the ee value is 99.7 percent.

Example 5

The genetically engineered Escherichia coli expressing the ω -transaminase of TA15-S303G obtained in example 1 was cultured by the method described in example 1 to obtain resting cells.

The reaction system was 100mL, containing 500mM substrate 1-methoxy-2-propanone, 500mM isopropylamine, 0.1mMPLP, and TA15-S303G resting cells (wet weight of cells after centrifugation at 4000rpm for 30 min: 1.0g) were added, the reaction temperature was controlled by water bath at 40 ℃ and the pH during the reaction was controlled at 8.5 by 20% aqueous isopropylamine solution. After 24h of reaction, the yield of 373.3mM of (S) -1-methoxy-2-propylamine is detected by gas chromatography; the concentration of the raw material 1-methoxy-2-acetone is 124.1mM, and the conversion rate is 75.2%; the detection product of the high performance liquid chromatography is mainly (S) -1-methoxy-2-propylamine, and the ee value is 99.6 percent.

Example 6

The engineered Escherichia coli expressing the ω -transaminase of TA15-F381V obtained in example 1 was cultured by the method described in example 1 to obtain about 2.0g of wet cells, which were disrupted to obtain a crude enzyme solution, which was then subjected to nickel column affinity chromatography (Biotech: 2016,32(7):912-926) to obtain TA15-F381V pure enzyme.

The reaction system is 100mL, contains 500mM substrate 1-methoxy-2-acetone, 1000mM isopropylamine, 0.2mM PLP, and is added with TA15-F381V pure enzyme, the reaction temperature is controlled to 30 ℃ by water bath, and the pH during the reaction is controlled to 8.0 by 20% isopropylamine aqueous solution. Reacting for 48h, and detecting the generation amount of 449.1mM of (S) -1-methoxy-2-propylamine by gas chromatography; the concentration of the raw material 1-methoxy-2-acetone is 44.3mM, and the conversion rate is 91.1%; the detection product of the high performance liquid chromatography is mainly (S) -1-methoxy-2-propylamine, and the ee value is 99.9 percent.

Example 7

The engineered Escherichia coli expressing the ω -transaminase of TA15-I392L obtained in example 1 was cultured by the method described in example 1 to obtain about 2.0g of wet cells, and the wet cells were disrupted to obtain a crude enzyme solution.

The reaction system is 100mL, contains 700mM substrate 1-methoxy-2-acetone, 750mM isopropylamine and 0.1mMPLP, and is added with TA15-I392L crude enzyme solution, the reaction temperature is controlled to be 30 ℃ by water bath, and the pH during the reaction is controlled to be 8.5 by 20% isopropylamine aqueous solution. Reacting for 96h, and detecting the generation amount of 589.3mM of (S) -1-methoxy-2-propylamine by using gas chromatography; the concentration of the raw material 1-methoxy-2-acetone is 104.0mM, and the conversion rate is 85.1%; the detection product of the high performance liquid chromatography is mainly (S) -1-methoxy-2-propylamine, and the ee value is 99.8 percent.

Example 8

The Escherichia coli genetic engineering bacteria expressing the omega-transaminase of TA15-D391E obtained in example 1 are cultured according to the method of example 1 to obtain wet cells of about 15.0g, the wet cells are broken to obtain crude enzyme liquid, then nickel column affinity chromatography (biological engineering report, 2016,32(7):912-926) is carried out to obtain TA15-D391E pure enzyme, and TA15-D391E pure enzyme is immobilized (the immobilization method is referred to as ChemCATChem chem,2012,4,1071-1074), so as to obtain immobilized TA 15-D391E.

The reaction system was 1000mL, 1.0mM PLP was added, TA15-D391E immobilized enzyme was added, 1500mM substrate 1-methoxy-2-propanone and 3000mM isopropylamine were fed continuously, the reaction temperature was controlled to 30 ℃ by a water bath, and the pH during the reaction was controlled to 8.0 by 20% aqueous isopropylamine solution. After the reaction for 36 hours, the generation amount of the (S) -1-methoxy-2-propylamine is 1029.3mM by gas chromatography detection; the concentration of the raw material 1-methoxy-2-acetone is 404.0mM, and the conversion rate is 73.0 percent; the detection product of the high performance liquid chromatography is mainly (S) -1-methoxy-2-propylamine, and the ee value is 99.9 percent.

Sequence listing

<110> Hangzhou international scientific center of Zhejiang university

<120> application of recombinant transaminase and mutant thereof in preparation of (S) -1-methoxy-2-propylamine

<160> 20

<170> SIPOSequenceListing 1.0

<210> 1

<211> 1431

<212> DNA

<213> Bacillus (Bacillus sp.)

<400> 1

atgagcctga cggtgcagaa aattaattgg gaacaggtga aagaatggga tcgcaaatat 60

ctgatgcgca cgtttagcac gcagaatgaa tatcagccgg ttccgattga aagcacggaa 120

ggtgattatc tgattatgcc ggatggtacc cgtctgctgg atttttttaa tcagctgtat 180

tgtgttaacc tgggtcagaa aaatccgaaa gttaatgcag caattaaaga agcactggat 240

cgttatggtt ttgtgtggga tacatatagc accgattata aagcaaaagc agcaaaaatc 300

atcatcgaag atattctggg cgatgaagat tggccgggta aagtgcgttt tgttagcacc 360

ggtagcgaag cagttgaaac agcactgaat attgcccgcc tgtatacaaa tcgccctctg 420

gttgtgacgc gtgaacatga ttatcatggt tggacaggtg gtgcagccac cgtaacccgt 480

ctgcgtagtt atcgtagcgg tctggttggt gaaaattctg aaagctttag cgctcagatt 540

ccgggcagca gctataatag cgccgttctg atggcaccga gcccgaatat gtttcaggat 600

agtaatggta attgcctgaa agatgaaaat ggcgaactgc tgagcgttaa atatacccgt 660

cgtatgattg aaaattatgg tccggaacag gttgcagcag taatcacgga agtaagtcag 720

ggtgcaggta gcgcaatgcc gccatatgaa tatattccgc agattcgcaa aatgaccaaa 780

gaactgggtg ttctgtggat caccgatgaa gtactgaccg gctttggtcg tacaggcaaa 840

tggtttggtt atcagcatta tggtgttcag ccggatatta ttacaatggg taaaggtctg 900

agcagcagta gcctgccggc aggtgcagtt ctggttagta aagaaattgc agaatttatg 960

gatcgtcatc gctgggaaag tgtgagcaca tatgcaggtc atccggtggc aatggcagca 1020

gtttgtgcaa atctggaagt gatgatggaa gaaaattttg ttgaacaggc aaaaaatagc 1080

ggtgaatata ttcgtagcaa actggaactg ctgcaggaaa aacataaaag cattggtaat 1140

tttgatggtt atggtctgct gtggattgtt gatattgtta atgcaaaaac caaaaccccg 1200

tatgttaaac tggatcgtaa ttttacccac ggtatgaatc cgaatcagat tccgacccag 1260

attattatga aaaaagcact ggaaaaaggt gttctgattg gtggtgttat gccgaatacc 1320

atgcgtattg gtgcaagcct gaatgttagc cgtgaagata ttgataaagc aatggatgca 1380

ctggattatg cactggatta tctggaaagc ggtgaatggc agcagagcta a 1431

<210> 2

<211> 476

<212> PRT

<213> Bacillus (Bacillus sp.)

<400> 2

Met Ser Leu Thr Val Gln Lys Ile Asn Trp Glu Gln Val Lys Glu Trp

1 5 10 15

Asp Arg Lys Tyr Leu Met Arg Thr Phe Ser Thr Gln Asn Glu Tyr Gln

20 25 30

Pro Val Pro Ile Glu Ser Thr Glu Gly Asp Tyr Leu Ile Met Pro Asp

35 40 45

Gly Thr Arg Leu Leu Asp Phe Phe Asn Gln Leu Tyr Cys Val Asn Leu

50 55 60

Gly Gln Lys Asn Pro Lys Val Asn Ala Ala Ile Lys Glu Ala Leu Asp

65 70 75 80

Arg Tyr Gly Phe Val Trp Asp Thr Tyr Ser Thr Asp Tyr Lys Ala Lys

85 90 95

Ala Ala Lys Ile Ile Ile Glu Asp Ile Leu Gly Asp Glu Asp Trp Pro

100 105 110

Gly Lys Val Arg Phe Val Ser Thr Gly Ser Glu Ala Val Glu Thr Ala

115 120 125

Leu Asn Ile Ala Arg Leu Tyr Thr Asn Arg Pro Leu Val Val Thr Arg

130 135 140

Glu His Asp Tyr His Gly Trp Thr Gly Gly Ala Ala Thr Val Thr Arg

145 150 155 160

Leu Arg Ser Tyr Arg Ser Gly Leu Val Gly Glu Asn Ser Glu Ser Phe

165 170 175

Ser Ala Gln Ile Pro Gly Ser Ser Tyr Asn Ser Ala Val Leu Met Ala

180 185 190

Pro Ser Pro Asn Met Phe Gln Asp Ser Asn Gly Asn Cys Leu Lys Asp

195 200 205

Glu Asn Gly Glu Leu Leu Ser Val Lys Tyr Thr Arg Arg Met Ile Glu

210 215 220

Asn Tyr Gly Pro Glu Gln Val Ala Ala Val Ile Thr Glu Val Ser Gln

225 230 235 240

Gly Ala Gly Ser Ala Met Pro Pro Tyr Glu Tyr Ile Pro Gln Ile Arg

245 250 255

Lys Met Thr Lys Glu Leu Gly Val Leu Trp Ile Thr Asp Glu Val Leu

260 265 270

Thr Gly Phe Gly Arg Thr Gly Lys Trp Phe Gly Tyr Gln His Tyr Gly

275 280 285

Val Gln Pro Asp Ile Ile Thr Met Gly Lys Gly Leu Ser Ser Ser Ser

290 295 300

Leu Pro Ala Gly Ala Val Leu Val Ser Lys Glu Ile Ala Glu Phe Met

305 310 315 320

Asp Arg His Arg Trp Glu Ser Val Ser Thr Tyr Ala Gly His Pro Val

325 330 335

Ala Met Ala Ala Val Cys Ala Asn Leu Glu Val Met Met Glu Glu Asn

340 345 350

Phe Val Glu Gln Ala Lys Asn Ser Gly Glu Tyr Ile Arg Ser Lys Leu

355 360 365

Glu Leu Leu Gln Glu Lys His Lys Ser Ile Gly Asn Phe Asp Gly Tyr

370 375 380

Gly Leu Leu Trp Ile Val Asp Ile Val Asn Ala Lys Thr Lys Thr Pro

385 390 395 400

Tyr Val Lys Leu Asp Arg Asn Phe Thr His Gly Met Asn Pro Asn Gln

405 410 415

Ile Pro Thr Gln Ile Ile Met Lys Lys Ala Leu Glu Lys Gly Val Leu

420 425 430

Ile Gly Gly Val Met Pro Asn Thr Met Arg Ile Gly Ala Ser Leu Asn

435 440 445

Val Ser Arg Glu Asp Ile Asp Lys Ala Met Asp Ala Leu Asp Tyr Ala

450 455 460

Leu Asp Tyr Leu Glu Ser Gly Glu Trp Gln Gln Ser

465 470 475

<210> 3

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

agcaggcggt gcaatgccgc cgtacgaata ta 32

<210> 4

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

gcattgcacc gcctgctccc tgacttacct cc 32

<210> 5

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 5

aatgtgtaag gagctgggtg tgctgtggat ta 32

<210> 6

<211> 41

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 6

ccagctcctt acacatttta cgaatctgag gaatatattc g 41

<210> 7

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

tgagcagtgg tagcctgccg gccggtgcag tt 32

<210> 8

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

caggctacca ctgctcagtc ccttacccat tg 32

<210> 9

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 9

ctgggtgcag ttctggtttc taaagaaatt gc 32

<210> 10

<211> 38

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 10

accagaactg cacccagcgg caggctacta ctgctcag 38

<210> 11

<211> 34

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 11

ccgcagcatg tgcaaattta gaagttatga tgga 34

<210> 12

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 12

atttgcacat gctgcggcca ttgcaaccgg at 32

<210> 13

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 13

aggtaacgtt gacggttatg gactgctgtg ga 32

<210> 14

<211> 37

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 14

aaccgtcaac gttacctatg cttttatgtt tttcctg 37

<210> 15

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 15

ctttcgtggt tatggactgc tgtggattgt gg 32

<210> 16

<211> 41

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 16

gtccataacc acgaaagtta cctatgcttt tatgtttttc c 41

<210> 17

<211> 42

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 17

gctgtggatt gtggaaattg ttaatgcaaa gacaaaaaca cc 42

<210> 18

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 18

tttccacaat ccacagcagt ccataaccgt ca 32

<210> 19

<211> 34

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 19

tgtggacctg gttaatgcaa agacaaaaac accg 34

<210> 20

<211> 32

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 20

cattaaccag gtccacaatc cacagcagtc ca 32

Claims

1. The application of transaminase in preparing (S) -1-methoxy-2-propylamine is characterized in that the amino acid sequence of the transaminase is shown as SEQ ID NO. 2.

2. The use of claim 1, wherein the gene encoding said transaminase has the nucleotide sequence shown in SEQ ID No. 1.

3. Application of genetically engineered bacteria in preparation of (S) -1-methoxy-2-propylamine is characterized in that the genetically engineered bacteria comprise transaminase genes with coding nucleotide sequences shown as SEQ ID No. 1.

4. A transaminase mutant, characterized in that the transaminase mutant is one of:

(1) mutating 303 th serine of amino acid shown in SEQ ID NO.2 into glycine;

5. A gene encoding the transaminase mutant of claim 3.

6. A recombinant vector comprising the coding gene of claim 4.

7. A genetically engineered bacterium comprising the gene encoding according to claim 4.

8. Use of the transaminase mutant as claimed in claim 4 or the genetically engineered bacterium as claimed in claim 7 for the preparation of (S) -1-methoxy-2-propylamine.

9. A process for the preparation of (S) -1-methoxy-2-propylamine which comprises: taking 1-methoxy-2-acetone as a substrate, and carrying out non-para-amidogen transfer reaction by using a catalyst in the presence of isopropylamine or isopropylamine salt and a cofactor to obtain (S) -1-methoxy-2-propylamine;

the catalyst is wild transaminase with an amino acid sequence shown as SEQ ID NO.2 or immobilized enzyme thereof, or a transaminase mutant or immobilized enzyme thereof as claimed in claim 4, or a genetically engineered bacterium as claimed in claim 7.

10. The process for the preparation of (S) -1-methoxy-2-propylamine according to claim 9, wherein the cofactor is pyridoxal phosphate, or pyridoxamine phosphate, or pyridoxine phosphate.