CN114133926A - Preparation method and application of uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence - Google Patents

Preparation method and application of uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence Download PDF

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CN114133926A
CN114133926A CN202111494145.8A CN202111494145A CN114133926A CN 114133926 A CN114133926 A CN 114133926A CN 202111494145 A CN202111494145 A CN 202111494145A CN 114133926 A CN114133926 A CN 114133926A
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魏燕霞
周建
庄在非
何桂强
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Southwest University of Science and Technology
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Abstract

The invention discloses a preparation method and application of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence, which comprises the following steps: preparing a corn protein ethanol solution, adding NaOH into the corn protein ethanol solution, stirring for reaction, adjusting the pH of the solution by using an HCl solution, then adding the solution into a dialysis bag for dialysis, adding the dialysis bag into a polyethylene glycol solution for concentration, and then drying the solution in the dialysis bag to obtain corn polypeptide; preparing a corn polypeptide aqueous solution; and adding the curcumin ethanol solution into the corn polypeptide aqueous solution under the condition of keeping out of the sun, and stirring and mixing to obtain the curcumin/corn polypeptide fluorescent probe. The method can rapidly detect the concentration of the uranyl ions in the water sample; the preparation method is simple in preparation process, low in cost and environment-friendly; the obtained fluorescent probe has high-efficiency and specific recognition capability on uranyl ions in the solution, and can be widely used for water quality detection of radioactive nuclear industrial wastewater, oceans, lakes, domestic water and the like.

Description

Preparation method and application of uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence
Technical Field
The invention relates to a preparation method of a fluorescent probe for detecting uranyl ions in a water body, belongs to the field of radionuclide detection, and particularly relates to a preparation method and application of a uranyl ion in-situ monitoring probe based on corn polypeptide sensitized curcumin fluorescence.
Background
Uranium is the most common radionuclide in nuclear waste, and has the characteristics of strong radioactivity, long half-life and high biological and chemical toxicity. In addition, uranium is chemically active and has a variety of forms in nature. Among them, uranyl ion (u (vi)) is considered to be the most stable existing form, and this form of u (vi) migrates and spreads to biosphere with surface water and ground water, causing its enrichment in living body, and finally enters human body through food chain to cause physiological lesion, thereby causing serious harm and injury to life activities of animals and human beings.
The detection of uranium plays an important role in national defense safety, nuclear energy development and environmental protection, and how to effectively treat and rapidly monitor uranium (u (vi)) in radioactive wastewater is one of the most concerned leading problems in radiochemical research. The currently available uranium detection technologies include Atomic Absorption Spectroscopy (AAS), inductively coupled plasma mass spectrometry (ICP-MS), inductively coupled plasma atomic emission spectroscopy (ICP-AES), and the like. However, the instruments used in the methods are expensive, complex to operate and large in size, and are not suitable for field detection. Among the numerous monitoring methods, the fluorescence method has the advantages of high cost performance, simple operation, no need of complex and expensive large-scale instruments and sample pretreatment processes, and the like, and is more suitable for U (VI) monitoring. In selecting the fluorescent material, organic materials, inorganic materials, biomolecules, small molecule materials, and the like are generally used. In contrast, a fluorescent adsorption material based on some small molecules contains specific functional groups, has good selectivity and sensitivity, and is considered to be a good fluorescent probe material, but the stability and the anti-interference capability of the small molecule probe are poor, and the fluorescent emission needs to be enhanced by other methods.
Curcumin is a hydrophobic polyphenol compound, has fluorescence emission in the wavelength range of 450-550nm, and can be quenched in the process of combining with uranyl ions. Therefore, the fluorescent probe material can be used for constructing a fluorescent probe material for detecting uranyl ions. However, curcumin has poor water solubility and is easily degraded by environmental factors such as ultraviolet rays, temperature, oxygen content and the like, and therefore, a proper carrier needs to be constructed to stabilize and enhance the fluorescence emission of curcumin. In the aspect of curcumin carrier construction, common systems include liposome, micelle, emulsion, protein and the like, but no report of fluorescent probe using zein as a curcumin carrier on uranyl ion detection exists.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for preparing a uranyl ion in-situ monitoring probe based on curcumin fluorescence sensitized with corn polypeptide, comprising the steps of:
step one, preparing corn polypeptide: preparing a corn protein ethanol solution, adding NaOH into the corn protein ethanol solution, stirring and reacting for 8-72 h, adjusting the pH of the solution to 5-7 by using an HCl solution, then adding the solution into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a polyethylene glycol solution for concentrating for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: 0.1-10 g.L of the composition-1The aqueous solution of corn polypeptide of (a); under the condition of keeping out of the sun, the concentration is 0.1-3 g.L-1The curcumin ethanol solution is added into the corn polypeptide aqueous solution, stirred and mixed to obtain the curcumin/corn polypeptide fluorescent probe.
Preferably, the concentration of the zein ethanol solution is 30-80 g.L-1(ii) a The volume fraction of the ethanol solution adopted in the preparation of the zein ethanol solution is 60-90%.
Preferably, the mass ratio of the NaOH to the zein in the zein ethanol solution is 1: 2-3; the concentration of the HCl solution is 4-6 mol/L.
Preferably, the concentration of the polyethylene glycol solution is 10-20 wt%.
Preferably, in the first step, the drying temperature is 35-45 ℃.
Preferably, in the second step, the volume ratio of the corn polypeptide aqueous solution to the curcumin ethanol solution is 3mL to 10 μ L.
Preferably, the process of the second step is replaced by: adding corn polypeptide into a stainless steel high-pressure reaction kettle provided with a stirrer and a pressure sensor, removing air in the kettle by using carbon dioxide, introducing the carbon dioxide, stirring for 30-45 min at 35-40 ℃ and 14-17 MPa, relieving pressure, and adding deionized water to prepare 0.1-10 g.L-1The corn polypeptide aqueous solution is added with the concentration of 0.1-3 g.L-1Adding the curcumin/corn polypeptide fluorescent probe, removing air in the kettle by using carbon dioxide under the condition of keeping out of the sun, introducing the carbon dioxide, and stirring for 45-60 min at the temperature of 35-40 ℃ and under the pressure of 14-17 MPa to obtain the curcumin/corn polypeptide fluorescent probe.
The invention also provides application of the curcumin/corn polypeptide fluorescent probe prepared by the preparation method in detecting uranyl ions in water, and the curcumin/corn polypeptide fluorescent probe is added into radioactive wastewater containing the uranyl ions and then subjected to fluorescence detection.
Preferably, the method further comprises the following steps: drawing a fluorescence detection standard curve: fluorescence intensity I for measuring curcumin/corn polypeptide fluorescent probe system under dark condition0And the fluorescence intensity I of the system after adding uranyl ion solutions with different concentrations is shown as I0And (4) drawing a standard curve by taking I/I as a vertical coordinate and the uranyl ion concentration as a horizontal coordinate, and calculating the uranyl ion concentration by measuring the change of the fluorescence intensity of a system.
Preferably, in the curcumin/corn polypeptide fluorescent probe system, the volume ratio of the corn polypeptide aqueous solution to the curcumin ethanol solution is 2.9mL to 10 muL; the concentration of the corn polypeptide aqueous solution is 0.1-10 g.L-1(ii) a The concentration of the curcumin ethanol solution is 0.1-3 g.L-1The added uranyl ion solution has a concentration of 0-500 g.L-1(ii) a Method for preparing corn polypeptide aqueous solution and uranyl ion solutionThe concentration is 2.9mL and 100 mu L; the water body containing the uranyl ions is any one of radioactive nuclear industrial wastewater, oceans, lakes and domestic water.
The invention at least comprises the following beneficial effects: (1) the method is simple to operate, avoids complex experimental steps, can quickly detect the concentration of the uranyl ions in the water sample, has response time of only a few seconds for detection, and has high detection speed; (2) the method has high accuracy and high stability, can accurately detect the concentration of the uranyl ions in the water sample, and has practical application prospect in the environmental water sample; (3) the preparation method is simple in preparation process, low in cost, green and environment-friendly, and avoids secondary pollution to the environment in the preparation process; the obtained fluorescent probe has high-efficiency and specific identification capability on uranyl ions in the solution, and can be widely used for water quality detection of radioactive nuclear industrial wastewater, oceans, lakes, domestic water and the like.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Description of the drawings:
FIG. 1 is a fluorescence spectrum of curcumin/maize polypeptide fluorescent probe prepared in embodiments 1-6 of the invention;
FIG. 2 is a fluorescence spectrum of curcumin/maize polypeptide fluorescent probe prepared in examples 7 to 13 of the present invention;
FIG. 3 is a fluorescence spectrum of curcumin/maize polypeptide fluorescent probe prepared in embodiments 14-20 of the present invention;
FIG. 4 is a fluorescence spectrum of curcumin/maize polypeptide fluorescent probes prepared in embodiments 21-31 of the present invention in uranyl ion solutions of different concentrations;
FIG. 5 is a standard curve of fluorescence spectra of curcumin/maize polypeptide fluorescent probes prepared in examples 21-31 of the present invention in uranyl ion solutions of different concentrations;
FIG. 6 is a fluorescence quenching ratio curve of curcumin/maize polypeptide fluorescent probes prepared in examples 21 to 31 of the present invention in uranyl ion solutions of different concentrations;
FIG. 7 is a photograph of fluorescence intensity of curcumin/maize polypeptide fluorescent probes prepared in examples 32 to 46 of the present invention in different interfering ion solutions;
FIG. 8 shows fluorescence intensity values of curcumin/maize polypeptide fluorescent probes prepared in embodiments 32 to 46 of the present invention in different interfering ion solutions;
FIG. 9 shows fluorescence intensity values of curcumin/maize polypeptide fluorescent probes prepared in embodiments 46 to 47 of the present invention in uranyl ion solutions;
FIG. 10 shows the fluorescence quenching ratios of curcumin/maize polypeptide fluorescent probes prepared in examples 32 to 46 of the present invention in different interfering ion solutions;
FIG. 11 shows the fluorescence quenching ratio of curcumin/maize polypeptide fluorescent probes prepared in examples 46 to 47 of the present invention in uranyl ion solution.
FIG. 12 is a sample of maize polypeptides prepared according to example 2 of the invention.
The specific implementation mode is as follows:
the present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 8 hours, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72 hours, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24 hours, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 1mg/L corn polypeptide aqueous solution; and under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 1mg/L into 3mL of corn polypeptide aqueous solution, and stirring and mixing for 60min to obtain the curcumin/corn polypeptide fluorescent probe.
Example 2:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 12h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 1mg/L corn polypeptide aqueous solution; and under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 1mg/L into 3mL of corn polypeptide aqueous solution, and stirring and mixing for 60min to obtain the curcumin/corn polypeptide fluorescent probe.
Example 3:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 24 hours, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72 hours, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24 hours, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 1mg/L corn polypeptide aqueous solution; and under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 1mg/L into 3mL of corn polypeptide aqueous solution, and stirring and mixing for 60min to obtain the curcumin/corn polypeptide fluorescent probe.
Example 4:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reacting for 36h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentrating for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 1mg/L corn polypeptide aqueous solution; and under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 1mg/L into 3mL of corn polypeptide aqueous solution, and stirring and mixing for 60min to obtain the curcumin/corn polypeptide fluorescent probe.
Example 5:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 48h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 1mg/L corn polypeptide aqueous solution; and under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 1mg/L into 3mL of corn polypeptide aqueous solution, and stirring and mixing for 60min to obtain the curcumin/corn polypeptide fluorescent probe.
Example 6:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 72h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 1mg/L corn polypeptide aqueous solution; and under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 1mg/L into 3mL of corn polypeptide aqueous solution, and stirring and mixing for 60min to obtain the curcumin/corn polypeptide fluorescent probe.
Performing fluorescence detection on the curcumin/corn polypeptide fluorescent probe prepared in the embodiment 1-6, (fluorescence spectrophotometer, excitation wavelength 425nm, measurement of fluorescence emission intensity of 450-700nm, as shown in figure 1);
examples 7 to 13:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 12h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 1mg/L into 3mL of corn polypeptide aqueous solution, stirring and mixing for 60min to obtain a curcumin/corn polypeptide fluorescent probe;
wherein the concentration of the aqueous solution of corn polypeptide is shown in table 1;
TABLE 1
Figure BDA0003399519050000071
Performing fluorescence detection on the curcumin/corn polypeptide fluorescent probe prepared in the embodiment 7-13, (fluorescence spectrophotometer, excitation wavelength 425nm, measurement of fluorescence emission intensity of 450-700nm, as shown in FIG. 2);
examples 14 to 20:
a preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 12h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 2mg/L corn polypeptide aqueous solution; adding 10 μ L of curcumin ethanol solution into 3mL of corn polypeptide aqueous solution under the condition of keeping out of the sun, stirring and mixing for 60min to obtain a curcumin/corn polypeptide fluorescent probe;
wherein the concentration of the aqueous solution of corn polypeptide is shown in table 2;
TABLE 2
Figure BDA0003399519050000081
Performing fluorescence detection on the curcumin/corn polypeptide fluorescent probe prepared in the example 14-20, (fluorescence spectrophotometer, excitation wavelength 425nm, measurement of fluorescence emission intensity of 450-700nm, as shown in FIG. 3);
examples 21 to 31:
an application of a uranyl ion in-situ monitoring probe based on curcumin fluorescence of corn polypeptide sensitization in detecting uranyl ions in radioactive wastewater comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 12h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 2mg/L corn polypeptide aqueous solution; under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 2mg/L into 2.9mL of corn polypeptide aqueous solution, stirring and mixing for 60min to obtain a curcumin/corn polypeptide fluorescent probe;
step three, adding 0.1mL of uranyl ion solutions with different concentrations into the curcumin/corn polypeptide fluorescent probe in the step two; performing fluorescence detection (fluorescence spectrophotometer, excitation wavelength 425nm, measuring fluorescence emission intensity of 450-700 nm); the results are shown in fig. 4 (the uranyl ion solution concentrations in fig. 4 are converted after adding uranyl ion solutions with different concentrations into curcumin/corn polypeptide fluorescent probes);
the concentrations of uranyl ions at different concentrations are shown in table 3, wherein no uranyl ion solution was added in example 21, and 0.1mL of ultrapure water was added for consistency;
TABLE 3
Figure BDA0003399519050000091
Drawing a fluorescence detection standard curve: fluorescence intensity I for measuring curcumin/corn polypeptide fluorescent probe system under dark condition0And the fluorescence intensity I of the system after adding uranyl ion solutions with different concentrations is shown as I0Drawing a standard curve by taking I/I as ordinate and uranyl ion concentration as abscissa, and measuring the fluorescence intensity of the systemCalculating the concentration of uranyl ions through degree change; namely, the concentration of uranyl ions in fig. 4 of 0-3.3mg/L and the corresponding fluorescence intensity are selected to draw a standard curve, as shown in fig. 5.
Examples 32 to 46:
an application of a uranyl ion in-situ monitoring probe based on curcumin fluorescence of corn polypeptide sensitization in detecting uranyl ions in radioactive wastewater comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 12h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 2mg/L corn polypeptide aqueous solution; under the condition of keeping out of the sun, adding 10 mu L of curcumin ethanol solution with the concentration of 2mg/L into 2.9mL of corn polypeptide aqueous solution, stirring and mixing for 60min to obtain a curcumin/corn polypeptide fluorescent probe (for fluorescence detection, a fluorescence spectrophotometer is used for exciting the wavelength of 425nm, the fluorescence emission intensity of 450-700nm is measured, the fluorescence intensity of 515nm is taken, and 0.1mL of ultrapure water is added into a blank corresponding to the blank);
step three, respectively adding 0.1mL of 1000 mu mol/L uranyl ion solution or 0.1mL of 2000 mu mol/L other interference ion solution or 0.1mL of mixed ion solution of the uranyl ion solution and the other interference ion solution into the curcumin/corn polypeptide fluorescent probe in the step two (wherein the concentration of the other interference ion solution is 2000 mu mol/L, and the concentration of the uranyl ion solution is 1000 mu mol/L); fluorescence detection was performed (fluorescence spectrophotometer, excitation wavelength 425nm, measurement of fluorescence emission intensity at 450-700nm, taking the fluorescence intensity at 515nm, as shown in FIGS. 7 and 8, in FIG. 7, CUR refers to the fluorescence intensity of ethanol solution of curcumin; FIG. 10 is the corresponding fluorescence quenching ratio, with higher fluorescence quenching ratio, better quenching effect).
Wherein the other interfering ions are shown in table 4;
TABLE 4
Figure BDA0003399519050000101
Example 47:
an application of a uranyl ion in-situ monitoring probe based on curcumin fluorescence of corn polypeptide sensitization in detecting uranyl ions in radioactive wastewater comprises the following steps:
step one, preparing corn polypeptide: preparing a zein ethanol solution (12.5g of zein is added into 250mL of 70% (v/v) ethanol solution), adding 5g of NaOH into the zein ethanol solution, stirring for reaction for 12h, adjusting the pH of the solution to 7 by using an HCl solution, then adding into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a 15 wt% polyethylene glycol solution for concentration for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: adding corn polypeptide into a stainless steel high-pressure reaction kettle provided with a stirrer and a pressure sensor, removing air in the kettle by using carbon dioxide, introducing the carbon dioxide, stirring for 30min at 35 ℃ and 16MPa, decompressing, adding deionized water to prepare 2.9mL of 2mg/L corn polypeptide aqueous solution, then adding 10 muL of 2mg/L curcumin ethanol solution, removing air in the kettle by using carbon dioxide, introducing the carbon dioxide, stirring for 45min at 35 ℃ and 16MPa under the condition of keeping out of the sun, and obtaining a curcumin/corn polypeptide fluorescent probe;
step three, adding 0.1mL of 1000 mu mol/L uranyl ion solution into the curcumin/corn polypeptide fluorescent probe in the step two; performing fluorescence detection (fluorescence spectrophotometer, excitation wavelength 425nm, measuring fluorescence emission intensity at 450-700nm, taking fluorescence intensity at 515nm, as shown in FIG. 9, and fluorescence quenching ratio as shown in FIG. 11);
while embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.

Claims (10)

1. A preparation method of a uranyl ion in-situ monitoring probe based on maize polypeptide sensitized curcumin fluorescence is characterized by comprising the following steps:
step one, preparing corn polypeptide: preparing a corn protein ethanol solution, adding NaOH into the corn protein ethanol solution, stirring and reacting for 8-72 h, adjusting the pH of the solution to 5-7 by using an HCl solution, then adding the solution into a dialysis bag and dialyzing in deionized water for 72h, then adding the dialysis bag into a polyethylene glycol solution for concentrating for 24h, and then drying the solution in the dialysis bag to obtain corn polypeptide;
step two, preparing a curcumin/corn polypeptide fluorescent probe: preparing 0.1-10 mg/L corn polypeptide aqueous solution; and under the condition of keeping out of the sun, adding the curcumin ethanol solution with the concentration of 0.1-3 mg/L into the corn polypeptide aqueous solution, and stirring and mixing to obtain the curcumin/corn polypeptide fluorescent probe.
2. The method for preparing the uranyl ion in-situ monitoring probe based on the maize polypeptide sensitized curcumin fluorescence according to claim 1, wherein the concentration of the zein ethanol solution is 30-80 g-L-1(ii) a The volume fraction of the ethanol solution adopted in the preparation of the zein ethanol solution is 60-90%.
3. The preparation method of the uranyl ion in-situ monitoring probe based on the maize polypeptide sensitized curcumin fluorescence, according to claim 1, wherein the mass ratio of NaOH to zein in a zein ethanol solution is 1: 2-3; the concentration of the HCl solution is 4-6 mol/L.
4. The preparation method of the uranyl ion in-situ monitoring probe based on the maize polypeptide sensitized curcumin fluorescence, according to claim 1, wherein the concentration of the polyethylene glycol solution is 10-20 wt%.
5. The preparation method of the uranyl ion in-situ monitoring probe based on the maize polypeptide sensitized curcumin fluorescence, according to claim 1, wherein in the first step, the drying temperature is 35-45 ℃.
6. The method for preparing the uranyl ion in-situ monitoring probe based on the sensitization of the corn polypeptide and the curcumin fluorescence according to claim 1, wherein in the second step, the volume ratio of the corn polypeptide aqueous solution to the curcumin ethanol solution is 3mL to 10 μ L.
7. The method for preparing the uranyl ion in-situ monitoring probe based on the maize polypeptide sensitized curcumin fluorescence, according to claim 1, is characterized in that the process of the second step is replaced by the following steps: adding corn polypeptide into a stainless steel high-pressure reaction kettle provided with a stirrer and a pressure sensor, removing air in the kettle by using carbon dioxide, introducing the carbon dioxide, stirring for 30-45 min at 35-40 ℃ and 14-17 MPa, relieving pressure, and adding deionized water to prepare 0.1-10 g.L-1The corn polypeptide aqueous solution is added with the concentration of 0.1-3 g.L-1Adding the curcumin/corn polypeptide fluorescent probe, removing air in the kettle by using carbon dioxide under the condition of keeping out of the sun, introducing the carbon dioxide, and stirring for 45-60 min at the temperature of 35-40 ℃ and under the pressure of 14-17 MPa to obtain the curcumin/corn polypeptide fluorescent probe.
8. The application of the curcumin/corn polypeptide fluorescent probe prepared by the preparation method of any one of claims 1 to 7 in detection of uranyl ions in radioactive wastewater is characterized in that the curcumin/corn polypeptide fluorescent probe is added into a water body containing the uranyl ions, and then fluorescence detection is carried out.
9. The curcumin/corn polypeptide fluorescent probe prepared by the preparation method according to claim 8 is used for detecting radioactive wastesThe application of uranyl ions in water is characterized by further comprising the following steps: drawing a fluorescence detection standard curve: fluorescence intensity I for measuring curcumin/corn polypeptide fluorescent probe system under dark condition0And the fluorescence intensity I of the system after adding uranyl ion solutions with different concentrations is shown as I0And (4) drawing a standard curve by taking I/I as a vertical coordinate and the uranyl ion concentration as a horizontal coordinate, and calculating the uranyl ion concentration by measuring the change of the fluorescence intensity of a system.
10. The application of the curcumin/corn polypeptide fluorescent probe prepared by the preparation method according to claim 8 in detecting uranyl ions in radioactive wastewater, wherein in the curcumin/corn polypeptide fluorescent probe system, the volume ratio of a corn polypeptide aqueous solution to a curcumin ethanol solution is 2.9mL:10 μ L; the concentration of the corn polypeptide aqueous solution is 0.1-10 g.L-1(ii) a The concentration of the curcumin ethanol solution is 0.1-3 g.L-1The added uranyl ion solution has a concentration of 0-500 mg.L-1(ii) a The concentration of the corn polypeptide aqueous solution and the uranyl ion solution is 2.9mL and 100 mu L; the water body containing the uranyl ions is any one of radioactive nuclear industrial wastewater, oceans, lakes and domestic water.
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