CN114130449B - Kit for detecting resistance allele of abortion disease of milk goats - Google Patents
Kit for detecting resistance allele of abortion disease of milk goats Download PDFInfo
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- CN114130449B CN114130449B CN202111398696.4A CN202111398696A CN114130449B CN 114130449 B CN114130449 B CN 114130449B CN 202111398696 A CN202111398696 A CN 202111398696A CN 114130449 B CN114130449 B CN 114130449B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/02—Water baths; Sand baths; Air baths
Abstract
The invention relates to the technical field of biology, in particular to a kit for detecting resistance allele of abortion disease of milk goats, which comprises a plurality of test tubes and also comprises: a reagent cartridge body; the partition plate is fixed on the inner side of the reagent box body; a shaking frame which is arranged at the inner side of the reagent box body, connecting shafts are fixedly arranged at two sides of the shaking frame far away from each other, connecting rings are fixedly arranged on one side in the kit body and one side of the partition plate, the connecting shafts can rotate on the inner sides of the connecting rings, and test tube bearing devices are arranged on the inner sides of the rocking frames; the base box is fixed on one side outside the kit body, and a buffering supporting device is arranged on one side of the base box. According to the invention, through arranging the reagent box body, the partition plate, the shaking frame, the connecting shaft, the connecting ring, the base box, the lifting rod, the control panel, the processing controller, the storage battery, the box cover, the U-shaped frame and the bearing plate, the reagent box has the functions of solidification prevention, automatic shaking, intelligent constant-temperature culture, uniform heating, buffer protection and the like.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a kit for detecting abortion disease resistance allele of a milk goat.
Background
The milk goat is a goat of a dairy variety, a good variety bred and bred by high breeding, and the milk yield is very high. The goat milk has comprehensive nutrition, is an important raw material suitable for drinking and modern dairy industry, and has become another bright point for enriching breeding with the coming of domestic goat milk consumption age. The dairy goat has the advantages of less concentrate demand, wide feed source, high reproduction rate, strong adaptability and easy feeding, and the allele is a gene which is positioned on the same position of a pair of homologous chromosomes and controls the same character and different forms. During the breeding period of the milk goats, the abortion disease resistance needs to be known, so the alleles of the milk goats need to be detected, and when the alleles of the milk goats are detected, the samples need to be sampled, then the samples are put into test tubes, and then a plurality of test tubes are put into a kit and brought back to a laboratory.
The kit in the prior art has no constant temperature culture function, and only can store test tubes with samples, so the samples can only be taken back to a laboratory and cultured, and the samples are detected after the culture is finished, so that the optimal culture time is possibly missed, the detection error is larger, the current kit has no functions of solidification prevention and automatic shaking, when the samples are stored in the reagent kit, the samples are easy to solidify in the test tubes, the subsequent use of the samples is inconvenient, and the samples need to be manually shaken by detection personnel, so the kit is inconvenient to use, and therefore, the kit for detecting the resistance allele of the abortion disease of the milk goats is provided and used for solving the problems.
Disclosure of Invention
The invention aims to solve the problems that a kit in the prior art has no constant temperature culture function, only can be used for storing a test tube with a sample, so that the sample can only be taken back to a laboratory, cultured and detected after the culture is finished, so that the optimal culture time is possibly missed, the detection error is larger, the conventional kit has no functions of solidification prevention and automatic shaking, when the sample is stored in the kit, the sample is easy to solidify in the test tube, so that the subsequent use of the sample is inconvenient, the sample is required to be manually shaken by a detection person, and the kit is inconvenient to use.
In order to achieve the purpose, the invention adopts the following technical scheme:
designing a milk goat abortion disease resistance allele detect reagent box, including a plurality of test tube, still include:
a reagent cartridge body;
the partition plate is fixed on the inner side of the reagent box body;
the reagent box comprises a reagent box body, a shaking frame, a connecting ring, a test tube bearing device and an intelligent constant-temperature culture device, wherein the shaking frame is arranged at the inner side of the reagent box body, connecting shafts are fixedly arranged at two sides of the shaking frame, which are far away from each other, respectively, a connecting ring is fixedly arranged at one side in the reagent box body and one side of a partition plate, the connecting shafts can rotate at the inner side of the connecting rings, the inner side of the shaking frame is provided with the test tube bearing device, and one side of the partition plate, which is far away from the shaking frame, is provided with the intelligent constant-temperature culture device;
the base box is fixed on one side outside the kit body, and a buffering supporting device is arranged on one side of the base box;
the lifting rod can slide on one side in the reagent box body, a guide hole is formed in one side, close to the base box, of the reagent box body, the lifting rod can slide on the inner side of the guide hole, a telescopic rotating connecting device is arranged between the lifting rod and the shaking frame, and an automatic reciprocating device is arranged between the lifting rod and the base box;
the control panel is fixed on one side outside the kit body;
the storage battery and the control panel are electrically connected with the processing controller through leads;
the lid, lid rotatable coupling is on reagent box body one side.
Preferably, the test tube bearing device comprises a U-shaped frame, the U-shaped frame is fixed on the inner side of the shaking frame, a bearing plate is fixedly arranged on the inner side of the U-shaped frame, a plurality of test tube holes are formed in one side of the bearing plate, a protection ring is fixedly arranged on the inner side of each test tube hole, a plurality of test tube grooves are formed in one side of the U-shaped frame, a protection sleeve is fixedly arranged on the inner side of each test tube groove, and the test tubes can be detached from the inner sides of the protection rings and the protection sleeves.
Preferably, a T-shaped sliding block is fixedly arranged on one side in the kit body, a T-shaped sliding groove is formed in one side of the lifting rod, and the T-shaped sliding block can slide on the inner side of the T-shaped sliding groove.
Preferably, intelligence constant temperature culture apparatus includes the heating box, the fixed carriage that is equipped with in heating box one side, the carriage is fixed on one side in the reagent box body, the fixed electric heating piece that is equipped with in one side that the heating box is close to the carriage, heating box one side is equipped with the through hole, the inboard fixed temperature sensor that is equipped with of through hole, temperature sensor, electric heating piece all pass through wire and processing controller electric connection, the fixed plate that is equipped with in one side that the carriage was kept away from to the heating box, fixed plate one side is equipped with a plurality of through-holes, the inboard fixed test tube seat that is equipped with of through-hole, the inboard packing of heating box has water bath heating liquid, the test tube seat inboard is all fixed and is equipped with the cotton wadding, a plurality of the test tube all can be dismantled inboard at the cotton wadding.
Preferably, buffering strutting arrangement includes the buffer board, the buffer board is established at base box inboard, fixed a plurality of springs, the attenuator of being equipped with between buffer board one side and the external one side of kit, the fixed two connecting plates that are equipped with in buffer board one side, connecting plate one side is all fixed and is equipped with two supporting legs, base box one side is equipped with four connecting holes, the supporting leg all can slide in the connecting hole inboard.
Preferably, scalable rotation connecting device includes the telescope tube, telescope tube one side is fixed on rocking frame one side, the inboard slidable of telescope tube is equipped with the telescopic link, the both sides that the telescope tube was kept away from mutually all are equipped with spacing hole, the both sides that the telescopic link was kept away from mutually all are fixed and are equipped with the stopper, the stopper all can slide in spacing downthehole side, the fixed U type seat that is equipped with in lifter one side, the telescopic link is inboard at U type seat through the rotatable formula connection of pivot.
Preferably, the automatic reciprocating device comprises a rotating shaft, a supporting seat is rotatably arranged on the outer side of the rotating shaft, one side of the supporting seat is fixed on one side in the base box, an electric motor is fixedly arranged on one side of the supporting seat and is electrically connected with a processing controller through a wire, the electric motor is connected with the rotating shaft through bevel gear transmission, a rotating disk is fixedly arranged on the outer side of the rotating shaft, a connecting rod is arranged on one side of the rotating disk through rotating shaft rotatable connection, and the connecting rod is rotatably connected with one side of a lifting rod through a rotating shaft.
Preferably, a dustproof sealing gasket is fixedly arranged on one side of the box cover, a connecting buckle is fixedly arranged on one side of the box cover, and the connecting buckle is detachably connected with the reagent box body.
Preferably, the test tube bearing device comprises a U-shaped frame, the U-shaped frame is fixed on the inner side of the shaking frame, a bearing plate is fixedly arranged on the inner side of the U-shaped frame, a plurality of test tube holes are formed in one side of the bearing plate, four inner rods are symmetrically and fixedly arranged in each test tube hole, an outer rod is arranged at one end, away from the side wall of each test tube hole, of each inner rod, a sliding groove is formed in one end, close to the inner rods, of each outer rod, one end, close to the outer rods, of each inner rod is connected with the inner side wall of the corresponding sliding groove in a sliding mode, and a first reset spring is arranged between the end portion of each inner rod and the end face of the corresponding sliding groove; an arc-shaped adjusting plate is fixedly arranged at one end, far away from the inner rod, of each outer rod, and a first protection pad is fixedly arranged on the surface of each adjusting plate;
and a plurality of test tube grooves are arranged on one side in the U-shaped frame, and the test tube grooves correspond to the test tube holes.
Preferably, a U-shaped air bag is fixedly arranged in each test tube groove, and a second protective pad is fixedly arranged on the surface of a groove at the bottom of the air bag; two first grooves are symmetrically formed in the left side and the right side of the air bag, sleeves are arranged at the first grooves, two ends of each sleeve are of an opening structure, one end, close to the inner side wall of the air bag, of each sleeve is communicated with the air bag, a piston plate is arranged in each sleeve in a sliding and sealing mode, a push rod is fixedly arranged on one end face, away from the inner side wall of the air bag, of each piston plate, a third protection pad is fixedly arranged at one end, away from the piston plate, of each push rod, and a second reset spring is further arranged between each piston plate and the air bag;
every the bottom of gasbag all communicates and is equipped with aerifys the branch pipe, and is a plurality of aerify the branch pipe all with aerify house steward and be connected, aerify the branch pipe with aerify house steward and all be located in the U type frame, just aerify house steward's one end and run through U type frame, aerify house steward runs through the one end of U type frame is connected with the air supply.
Has the advantages that:
1. the kit for detecting the resistance allele of the abortion disease of the milk goats, provided by the invention, has the beneficial effects that: through setting up reagent box body, baffle, rocking frame, connecting axle, go-between, base box, lifter, flexible cover, telescopic link, spacing hole, stopper, U type seat, rotation axis, supporting seat, electric motor, rotary disk, connecting rod, make it have prevent solidifying, shake even function automatically, can prevent that the sample in the test tube from taking place to solidify during depositing to be convenient for follow-up to the use of sample, and can shake even with the sample in the test tube automatically, need not to detect artifical manual shake even, convenient to use. Through setting up U type frame, loading board, guard ring, lag, make it be convenient for access test tube, and can protect the test tube. Through setting up control panel, process control ware, battery, heating box, carriage, electrical heating piece, temperature sensor, fixed plate, test tube seat, cotton-padded covering, make it have intelligent constant temperature and cultivate, the even heating function, can carry out the constant temperature to the sample in the test tube in the cotton-padded covering and cultivate to can prevent to miss the best sample and cultivate the time, improve the detection accuracy greatly, and can also adjust the cultivation temperature through control panel, can satisfy the operation requirement of different samples. Through setting up buffer board, spring, attenuator, connecting plate, supporting leg, make it have buffering protect function, can prevent that the test tube from removing, mentioning, putting down etc. in-process at reagent box body and taking place to damage, improve its security greatly. Through setting up lid, dustproof sealing pad, connector link, handle, make it have dustproof leakproofness, can prevent that external dust from getting into that the kit is internal to cause the pollution to the sample, and convenient to use.
2. According to the test tube bearing device, each test tube hole is provided with the adjustable structure in which the inner rod and the outer rod are mutually sleeved, so that the position of the arc-shaped adjusting plate can be adjusted, test tubes with different sizes can be conveniently fixed, and meanwhile, the test tube bearing device can play a role in buffering in the oscillation process; correspondingly, set up the gasbag in every test tube groove, aerify in the sleeve through to the gasbag and with the gasbag intercommunication, can promote about push rod and third protection pad to fix the not unidimensional test tube bottom. This test tube bears device can be used for fixing not unidimensional test tube to vibrate the in-process at the test tube and play the cushioning effect, avoid vibrating the in-process and damage the test tube.
Drawings
FIG. 1 is a schematic front perspective view of a kit for detecting abortion disease resistance alleles of milk goats, according to the present invention;
FIG. 2 is a schematic diagram of a front cut-away three-dimensional structure of the kit for detecting the abortion disease resistance allele of the milk goat;
FIG. 3 is a schematic view of a portion of the enlarged structure of the area A in FIG. 2;
FIG. 4 is a schematic side sectional perspective view of a kit for detecting an allele of abortion disease resistance in a milk goat, provided by the invention;
FIG. 5 is a schematic view of a partially enlarged structure of the area B in FIG. 4 according to the present invention;
FIG. 6 is a schematic view of a portion of the enlarged structure of the region C in FIG. 4;
FIG. 7 is a schematic diagram of a back-cut three-dimensional structure of the kit for detecting the abortion disease resistance allele of the milk goat.
Fig. 8 is a front view of the internal structure of the test tube carrier according to the second embodiment of the present invention.
FIG. 9 is a partially enlarged structural view of the region D in FIG. 8 according to the present invention.
Fig. 10 is a front view of the internal structure of the test tube slot of the test tube carrier according to the second embodiment of the present invention.
Fig. 11 is a top view of an internal structure of a test tube hole of a test tube carrier according to a second embodiment of the present invention.
In the figure: 1 test tube, 2 reagent box body, 3 baffle, 4 rock frame, 5 connecting shaft, 6 connecting ring, 7 base box, 8 lifting rod, 9 control panel, 10 processing controller, 11 storage battery, 12 box cover, 13U-shaped frame, 14 bearing plate, 15 protection ring, 16 protection cover, 17T-shaped slide block, 18 heating box, 19 support frame, 20 electric heating sheet, 21 water temperature sensor, 22 fixing plate, 23 test tube base, 24 cotton sleeve, 25 buffer plate, 26 spring, 27 damper, 28 connecting plate, 29 support leg, 30 expansion sleeve, 31 expansion link, 32 limit hole, 33 limit block, 34U-shaped seat, 35 rotating shaft, 36 support base, 37 electric motor, 38 rotating disk, 39 connecting rod, 40 dustproof sealing gasket, 41 connecting inner rod buckle, 42 handle, 43, 44 outer rod, 45 sliding groove, 46 first reset spring, 47 adjusting plate, 48 first protection pad, 49 air bag, 50 second protection pad, 51 first groove, 52 sleeve, 53 piston plate, 54 push rod, 55-third protection pad, 56, 57 inflation manifold, 57, 58 second protection pad.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The first embodiment is as follows:
referring to fig. 1-7, a kit for detecting abortion disease resistance allele of milk goat, includes a plurality of test tubes 1, and further includes:
a reagent cartridge body 2;
the partition plate 3 is fixed on the inner side of the reagent box body 2;
the reagent box comprises a shaking frame 4, the shaking frame 4 is arranged on the inner side of a reagent box body 2, connecting shafts 5 are fixedly arranged on two sides of the shaking frame 4 away from each other, connecting rings 6 are fixedly arranged on one side in the reagent box body 2 and one side of a partition plate 3, the connecting shafts 5 can rotate on the inner sides of the connecting rings 6, a test tube bearing device is arranged on the inner side of the shaking frame 4, and an intelligent constant-temperature culture device is arranged on one side of the partition plate 3 away from the shaking frame 4;
the base box 7 is fixed on one side outside the reagent box body 2, and a buffer supporting device is arranged on one side of the base box 7;
the lifting rod 8 can slide on one side in the reagent box body 2, a guide hole is formed in one side, close to the base box 7, of the reagent box body 2, the lifting rod 8 can slide on the inner side of the guide hole, a telescopic rotating connecting device is arranged between the lifting rod 8 and the shaking frame 4, and an automatic reciprocating device is arranged between the lifting rod 8 and the base box 7;
a control panel 9, wherein the control panel 9 is fixed on one side of the outer side of the reagent box body 2 (the control panel 9 in the present application is the prior art, and the detailed functions and the using mode thereof are not described herein any more);
the treatment controller 10, the storage battery 11, the treatment controller 10, and the storage battery 11 are all fixed on the inner side of the base box 7, and the storage battery 11 and the control panel 9 are both electrically connected with the treatment controller 10 through wires (the treatment controller 10 and the storage battery 11 in the present application are both in the prior art, and the detailed functions and use modes thereof are not described herein again);
a box cover 12, wherein the box cover 12 is rotatably connected on one side of the reagent box body 2.
The test tube bearing device comprises a U-shaped frame 13, the U-shaped frame 13 is fixed on the inner side of a shaking frame 4, a bearing plate 14 is fixedly arranged on the inner side of the U-shaped frame 13, a plurality of test tube holes are formed in one side of the bearing plate 14, a protection ring 15 is fixedly arranged on the inner side of each test tube hole, a plurality of test tube grooves are formed in one side of the U-shaped frame 13, a protection sleeve 16 is fixedly arranged on the inner side of each test tube groove, and the test tubes 1 can be detached from the inner sides of the protection ring 15 and the protection sleeve 16.
A T-shaped sliding block 17 is fixedly arranged on one side in the reagent box body 2, a T-shaped sliding groove is formed in one side of the lifting rod 8, and the T-shaped sliding block 17 can slide on the inner side of the T-shaped sliding groove.
Intelligence constant temperature culture apparatus includes heating box 18, the fixed carriage 19 that is equipped with in heating box 18 one side, carriage 19 is fixed on one side in reagent box body 2, heating box 18 is close to the fixed electric heating plate 20 that is equipped with in one side of carriage 19, heating box 18 one side is equipped with the through hole, the inboard fixed temperature sensor 21 that is equipped with of through hole, temperature sensor 21, electric heating plate 20 all passes through wire and processing controller 10 electric connection, the fixed plate 22 that is equipped with in one side that supporting frame 19 was kept away from to heating box 18, fixed plate 22 one side is equipped with a plurality of through-holes, the inboard all fixed test tube seat 23 that is equipped with of through-hole, 18 inboard packing of heating box has the water bath heating fluid, the inboard all fixed cotton-padded jacket 24 that is equipped with of test tube seat 23, a plurality of test tube 1 all can be dismantled in cotton-padded jacket 24 inboardly. (the electric heating sheet 20 and the water temperature sensor 21 in this application are both the prior art, and the specific functions and the using manner thereof are not described herein again).
Buffering strutting arrangement includes buffer board 25, and buffer board 25 is established at base box 7 inboardly, and fixed a plurality of springs 26, the attenuator 27 of being equipped with between buffer board 25 one side and the outer one side of reagent box body 2, and buffer board 25 one side is fixed to be equipped with two connecting plates 28, and connecting plate 28 one side is all fixed to be equipped with two supporting legs 29, and base box 7 one side is equipped with four connecting holes, and supporting leg 29 all can slide in the connecting hole inboard.
Scalable rotation connecting device includes flexible cover 30, and flexible cover 30 one side is fixed on rocking frame 4 one side, and the inboard slidable of flexible cover 30 is equipped with telescopic link 31, and the both sides that flexible cover 30 kept away from mutually all are equipped with spacing hole 32, and the both sides that telescopic link 31 kept away from mutually all are fixed and are equipped with stopper 33, and stopper 33 all can slide in spacing hole 32 inboardly, and 8 one sides of lifter are fixed and are equipped with U type seat 34, and telescopic link 31 is inboard at U type seat 34 through the rotatable formula connection of pivot.
The automatic reciprocating device comprises a rotating shaft 35, a supporting seat 36 is rotatably arranged on the outer side of the rotating shaft 35, one side of the supporting seat 36 is fixed on one side in a base box 7, an electric motor 37 is fixedly arranged on one side of the supporting seat 36, the electric motor 37 is electrically connected with the processing controller 10 through a conducting wire, the electric motor 37 is in transmission connection with the rotating shaft 35 through a bevel gear, a rotating disk 38 is fixedly arranged on the outer side of the rotating shaft 35, a connecting rod 39 is rotatably connected on one side of the rotating disk 38 through a rotating shaft, and the connecting rod 39 is rotatably connected with one side of the lifting rod 8 through a rotating shaft.
A dustproof sealing gasket 40 is fixedly arranged on one side of the box cover 12, a connecting buckle 41 is fixedly arranged on one side of the box cover 12, and the connecting buckle 41 is detachably connected with the reagent box body 2.
A handle 42 is rotatably connected to one side of the box cover 12.
When in use, the invention:
through setting up reagent box body 2, baffle 3, rock frame 4, connecting axle 5, go-between 6, base box 7, lifter 8, telescopic sleeve 30, telescopic link 31, spacing hole 32, stopper 33, U type seat 34, rotation axis 35, supporting seat 36, electric motor 37, rotary disk 38, connecting rod 39, make it have and prevent solidifying, shake even function automatically, can prevent that the sample in test tube 1 from taking place to solidify during depositing, thereby be convenient for follow-up use to the sample, and can shake even with the sample in test tube 1 automatically, need not to detect artifical manual shake even, and convenient to use, during concrete implementation: when the electric motor 37 rotates, the rotating shaft 35 and the rotating disc 38 are driven to rotate, the rotating disc 38 can make the lifting rod 8 do automatic reciprocating lifting motion through the connecting rod 39 when rotating, and the lifting rod 8 can be driven to rock the frame 4 through the telescopic sleeve 30, the telescopic rod 31 and the U-shaped seat 3 when doing automatic reciprocating lifting motion to do automatic reciprocating swinging motion, so that solidification prevention and automatic uniform shaking can be realized.
Through setting up U type frame 13, loading board 14, guard ring 15, lag 16, make it be convenient for access test tube 1, and can protect test tube 1, during the concrete implementation: when test tube 1 is required to be stored on U-shaped frame 13, test tube 1 only needs to be inserted into protective ring 15 and protective sleeve 16, and test tube 1 only needs to be directly pulled out when being taken away, so that the test tube is convenient to use.
Through setting up control panel 9, process control ware 10, battery 11, heating box 18, carriage 19, electric heating plate 20, water temperature sensor 21, fixed plate 22, test tube seat 23, cotton cover 24, make it have intelligent constant temperature cultivation, the even heating function, can carry out constant temperature cultivation to the sample in the test tube 1 in the cotton cover 24, thereby can prevent to miss the best sample and cultivate the time, and can also adjust the cultivation temperature through control panel 9, can satisfy the operation requirement of different samples, during the concrete implementation: the inboard packing of heating box 18 has the water bath heating fluid, when the sample is cultivateed to needs, processing controller 10 can control electric heating plate 20 according to the settlement temperature, thereby can carry out the self-heating to the water bath heating fluid in the heating box 18, and water temperature sensor 21 can carry out real-time supervision to the temperature of the interior water bath heating fluid of heating box 18, and water temperature sensor 21 can transmit temperature information to processing controller 10, processing controller 10 can carry out the self-control to electric heating plate 20 according to temperature information, thereby realize intelligent constant temperature heating and cultivate, and adopt the mode of water bath heating, can carry out the even heating to the sample in the test tube 1.
Through setting up buffer board 25, spring 26, attenuator 27, connecting plate 28, supporting leg 29, make it have buffering protect function, can prevent that test tube 1 from removing, mentioning, putting down etc. in-process at reagent box body 2 and taking place to damage, make it have higher security.
Through setting up lid 12, dustproof sealed pad 40, connector link 41, handle 42, make it have dustproof leakproofness, can prevent that external dust from getting into in the reagent box body 2 and causing the pollution to the sample, and convenient to use.
Example two:
on the basis of the first embodiment, the structure of the test tube carrier is further modified, as shown in fig. 8 to 11, so that the test tube carrier can be applied to different test tubes, and other technical features and the working principle of the test kit are the same as those of the first embodiment.
Specifically, the test tube bearing device comprises a U-shaped frame 13, the U-shaped frame 13 is fixed on the inner side of the shaking frame 4, a bearing plate 14 is fixedly arranged on the inner side of the U-shaped frame 13, a plurality of test tube holes are formed in one side of the bearing plate 14, four inner rods 43 are symmetrically and fixedly arranged in each test tube hole, an outer rod 44 is arranged at one end, away from the side wall of each test tube hole, of each inner rod 43, a sliding groove 45 is formed in one end, close to the inner rod 43, of each outer rod 44, one end, close to the outer rod 44, of each inner rod 43 is connected with the inner side wall of the sliding groove 45 in a sliding mode, and a first return spring 46 is arranged between the end part of each inner rod 43 and the end face of the sliding groove 45; an arc-shaped adjusting plate 47 is fixedly arranged at one end, far away from the inner rod 43, of each outer rod 44, the top of each adjusting plate 47 adopts a horn-shaped structure with a large top and a small bottom, so that a test tube can be conveniently inserted into the adjusting plate 47, and a first protective pad 48 is fixedly arranged on the surface of each adjusting plate 47; when the test tube is inserted, the test tube can extrude the adjusting plate 47 to the periphery, so that the four outer rods 44 are driven to move to the periphery, the first return spring 46 is extruded at the same time, after the test tube is inserted, the first return spring 46 pushes the outer rods 44 and the adjusting plate 47 under the deformation effect to extrude and fix the test tube, and the first protection pad 48 arranged on the surface of the adjusting plate 47 plays a role in protecting the test tube, so that the test tube is prevented from being damaged in the extruding process; simultaneously, first reset spring 46 can also play the cushioning effect at the in-process that whole test tube bears the device to vibrate, avoids damaging the test tube.
Furthermore, a plurality of test tube grooves are arranged on one side in the U-shaped frame 13, and the test tube grooves correspond to the test tube holes; a U-shaped air bag 49 is fixedly arranged in each test tube groove, and a second protective pad 50 is fixedly arranged on the surface of a groove at the bottom of the air bag 49; two first grooves 51 are symmetrically arranged on the left side and the right side of the air bag 49, a sleeve 52 is arranged at the position of each first groove 51, both ends of each sleeve 52 are of an open structure, one end, close to the inner side wall of the air bag 49, of each sleeve 52 is communicated with the air bag 49, a piston plate 53 is arranged in each sleeve 52 in a sliding and sealing mode, a push rod 54 is fixedly arranged on one end face, far away from the inner side wall of the air bag 49, of each piston plate 53, and a third protection pad 55 is fixedly arranged on one end, far away from the piston plate 53, of each push rod 54; a second return spring 59 is also arranged between the piston plate 53 and the air bag 49; after being full of gas in to gasbag 49, continue to inflate, gas gets into in the sleeve 52 with gasbag 49 intercommunication, promote piston plate 53 and remove, thereby promote push rod 54 and third protection pad 55 and remove to the center of test tube groove, fix the test tube bottom, meanwhile, second reset spring 59 is stretched, when the test tube is taken out to needs, emit the gas in the gasbag 49, second reset spring 59 resumes deformation, thereby pull piston plate 53 to the normal position, third protection pad 55 is pulled open thereupon, can take out the test tube, when placing the test tube, with the least significant of test tube and the upper surface contact of second protection pad 50 can. It should be noted that the elastic strength of the second return spring 59 can be selected according to actual conditions, so that the second piston plate 53 can be pushed away when the air bag 49 is inflated.
Each bottom of gasbag 49 all communicates and is equipped with inflation branch pipe 56, and is a plurality of inflation branch pipe 56 all is connected with inflation house steward 57, inflation branch pipe 56 with inflation house steward 57 all is located in U type frame 13, just inflation house steward 57's one end is run through U type frame 13, inflation house steward 57 runs through the one end of U type frame 13 is connected with air supply 58.
The concrete working principle of the test tube bearing device is as follows: when inserting the test tube, the test tube can be to extrusion regulating plate 47 all around, thereby drive four outer poles 44 and remove all around, extrude first reset spring 46 simultaneously, treat the test tube and insert the back, first reset spring 46 pushes outer pole 44 and regulating plate 47 under the deformation effect, it is fixed to extrude the test tube, treat the bottommost end of test tube and the upper surface contact back of second protection pad 50, start air supply 58, aerify in to each gasbag 49 through aerifing house steward 57 and aerifing branch pipe 56, after being full of gas in to gasbag 49, continue to aerify, gas gets into in the sleeve 52 with gasbag 49 intercommunication, promote piston plate 53 and remove, thereby promote push rod 54 and third protection pad 55 and remove to the center in test tube groove, fix the test tube bottom.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (4)
1. The utility model provides a milk goat abortion disease resistance allele detect reagent box, includes a plurality of test tube (1), its characterized in that still includes:
a reagent cartridge body (2);
the partition plate (3), the said partition plate (3) is fixed inside the reagent box body (2);
the reagent box comprises a shaking frame (4), wherein the shaking frame (4) is arranged on the inner side of a reagent box body (2), connecting shafts (5) are fixedly arranged on two sides, far away from the shaking frame (4), of the reagent box body (2), connecting rings (6) are fixedly arranged on one side in the reagent box body (2) and one side of a partition plate (3), the connecting shafts (5) can rotate on the inner sides of the connecting rings (6), a test tube bearing device is arranged on the inner side of the shaking frame (4), and an intelligent constant-temperature culture device is arranged on one side, far away from the shaking frame (4), of the partition plate (3);
the base box (7) is fixed on one side outside the reagent box body (2), and a buffering supporting device is arranged on one side of the base box (7);
the lifting rod (8) can slide on one side in the reagent box body (2), a guide hole is formed in one side, close to the base box (7), of the reagent box body (2), the lifting rod (8) can slide on the inner side of the guide hole, a telescopic rotating connecting device is arranged between the lifting rod (8) and the shaking frame (4), and an automatic reciprocating device is arranged between the lifting rod (8) and the base box (7);
the control panel (9), the said control panel (9) is fixed on one side outside the box body of reagent (2);
the device comprises a processing controller (10) and a storage battery (11), wherein the processing controller (10) and the storage battery (11) are fixed on the inner side of a base box (7), and the storage battery (11) and a control panel (9) are electrically connected with the processing controller (10) through leads;
the box cover (12), the box cover (12) can be rotatably connected to one side of the reagent box body (2);
the test tube bearing device comprises a U-shaped frame (13), the U-shaped frame (13) is fixed on the inner side of the shaking frame (4), a bearing plate (14) is fixedly arranged on the inner side of the U-shaped frame (13), a plurality of test tube holes are formed in one side of the bearing plate (14), protective rings (15) are fixedly arranged on the inner sides of the test tube holes, a plurality of test tube grooves are formed in one side of the U-shaped frame (13), protective sleeves (16) are fixedly arranged on the inner sides of the test tube grooves, and the test tubes (1) can be detached from the inner sides of the protective rings (15) and the protective sleeves (16);
a T-shaped sliding block (17) is fixedly arranged on one side in the reagent box body (2), a T-shaped sliding groove is formed in one side of the lifting rod (8), and the T-shaped sliding block (17) can slide on the inner side of the T-shaped sliding groove;
the intelligent constant-temperature culture device comprises a heating box (18), wherein a supporting frame (19) is fixedly arranged on one side of the heating box (18), the supporting frame (19) is fixed on one side in a reagent box body (2), an electric heating sheet (20) is fixedly arranged on one side, close to the supporting frame (19), of the heating box (18), a through hole is formed in one side of the heating box (18), a water temperature sensor (21) is fixedly arranged on the inner side of the through hole, the water temperature sensor (21) and the electric heating sheet (20) are electrically connected with a processing controller (10) through wires, a fixing plate (22) is fixedly arranged on one side, far away from the supporting frame (19), of the heating box (18), a plurality of through holes are formed in one side of the fixing plate (22), test tube seats (23) are fixedly arranged on the inner sides of the through holes, a water bath heating liquid is filled in the inner side of the heating box (18), cotton sleeves (24) are fixedly arranged on the inner sides of the test tube seats (23), and a plurality of test tubes (1) can be detached from the inner sides of the cotton sleeves (24);
the buffer supporting device comprises a buffer plate (25), the buffer plate (25) is arranged on the inner side of the base box (7), a plurality of springs (26) and dampers (27) are fixedly arranged between one side of the buffer plate (25) and the outer side of the reagent box body (2), two connecting plates (28) are fixedly arranged on one side of the buffer plate (25), two supporting legs (29) are fixedly arranged on one side of each connecting plate (28), four connecting holes are formed in one side of the base box (7), and the supporting legs (29) can slide on the inner sides of the connecting holes;
the telescopic rotary connecting device comprises a telescopic sleeve (30), one side of the telescopic sleeve (30) is fixed on one side of the shaking frame (4), a telescopic rod (31) is slidably arranged on the inner side of the telescopic sleeve (30), both sides of the telescopic sleeve (30) far away from each other are respectively provided with a limiting hole (32), both sides of the telescopic rod (31) far away from each other are respectively fixedly provided with a limiting block (33), the limiting blocks (33) can be slidably arranged on the inner sides of the limiting holes (32), one side of the lifting rod (8) is fixedly provided with a U-shaped seat (34), and the telescopic rod (31) is rotatably connected to the inner side of the U-shaped seat (34) through a rotating shaft;
automatic round trip gear includes rotation axis (35), the rotatable supporting seat (36) that is equipped with in rotation axis (35) outside, one side is fixed in base box (7) on one side supporting seat (36), fixed electric motor (37) that is equipped with in supporting seat (36) one side, electric motor (37) pass through wire and process controller (10) electric connection, be connected through bevel gear transmission between electric motor (37) and rotation axis (35), rotation axis (35) outside is fixed and is equipped with rotary disk (38), rotary disk (38) one side is equipped with connecting rod (39) through pivot rotatable coupling, connecting rod (39) are connected through the rotatable formula in pivot and lifter (8) one side.
2. The kit for detecting the alleles resistant to the abortion disease of the milk goat as claimed in claim 1, wherein a dustproof sealing gasket is fixedly arranged at one side of the box cover (12)
(40) The reagent box is characterized in that a connecting buckle (41) is fixedly arranged on one side of the box cover (12), and the connecting buckle (41) is detachably connected with the reagent box body (2).
3. The milk goat abortion disease resistance allele detection kit as claimed in claim 1, wherein the test tube carrying device comprises a U-shaped frame (13), the U-shaped frame (13) is fixed inside the shaking frame (4), a carrying plate (14) is fixed inside the U-shaped frame (13), one side of the carrying plate (14) is provided with a plurality of test tube holes, four inner rods (43) are symmetrically and fixedly arranged in each test tube hole, one end of each inner rod (43) far away from the side wall of each test tube hole is provided with an outer rod (44), one end of each outer rod (44) close to the inner rod (43) is provided with a sliding groove (45), one end of each inner rod (43) close to the outer rod (44) is slidably connected with the inner side wall of the sliding groove (45), and a first return spring (46) is arranged between the end of the inner rod (43) and the end face of the sliding groove (45); an arc-shaped adjusting plate (47) is fixedly arranged at one end, far away from the inner rod (43), of each outer rod (44), and a first protection pad (48) is fixedly arranged on the surface of each adjusting plate (47);
a plurality of test tube grooves are formed in one side in the U-shaped frame (13), and the test tube grooves correspond to the test tube holes.
4. The kit for detecting the abortion disease resistance allele of the milk goat as claimed in claim 3, wherein a U-shaped air bag (49) is fixedly arranged in each test tube groove, and a second protective pad (50) is fixedly arranged on the surface of a bottom groove of the air bag (49); the left side and the right side of the air bag (49) are symmetrically provided with two first grooves (51), a sleeve (52) is arranged at the position of each first groove (51), two ends of each sleeve (52) are of an open structure, one end, close to the inner side wall of the air bag (49), of each sleeve (52) is communicated with the air bag (49), a piston plate (53) is arranged in each sleeve (52) in a sliding and sealing mode, a push rod (54) is fixedly arranged on one end face, far away from the inner side wall of the air bag (49), of each piston plate (53), and a third protection pad (55) is fixedly arranged at one end, far away from the piston plate (53), of each push rod (54); a second return spring (59) is arranged between the piston plate (53) and the air bag (49);
every the bottom of gasbag (49) all communicates and is equipped with inflation branch pipe (56), and is a plurality of inflation branch pipe (56) all is connected with inflation house steward (57), inflation branch pipe (56) with inflation house steward (57) all is located in U type frame (13), just the one end of inflation house steward (57) is run through U type frame (13), inflation house steward (57) run through the one end of U type frame (13) is connected with air supply (58).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111398696.4A CN114130449B (en) | 2021-11-19 | 2021-11-19 | Kit for detecting resistance allele of abortion disease of milk goats |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111398696.4A CN114130449B (en) | 2021-11-19 | 2021-11-19 | Kit for detecting resistance allele of abortion disease of milk goats |
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| CN114130449A CN114130449A (en) | 2022-03-04 |
| CN114130449B true CN114130449B (en) | 2023-02-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111398696.4A Active CN114130449B (en) | 2021-11-19 | 2021-11-19 | Kit for detecting resistance allele of abortion disease of milk goats |
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| CN116040107B (en) * | 2023-03-03 | 2023-11-28 | 南京帝基生物科技有限公司 | Kit for genetic colorectal cancer gene detection and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5376313A (en) * | 1992-03-27 | 1994-12-27 | Abbott Laboratories | Injection molding a plastic assay cuvette having low birefringence |
| US5971599A (en) * | 1998-03-05 | 1999-10-26 | Bothers; Ron Scott | Reciprocating shaker apparatus |
| CN105457542B (en) * | 2016-01-12 | 2018-07-10 | 冯丹 | Lift oscillator |
| CN206177956U (en) * | 2016-11-11 | 2017-05-17 | 上海碧云天生物技术有限公司 | Lactate dehydrogenase toxicity detection kit |
| CN106563380B (en) * | 2016-11-11 | 2019-05-07 | 浙江探针生物科技有限公司 | A kind of kit with agitating function |
| CN107377040B (en) * | 2017-03-08 | 2019-09-13 | 黄思俊 | A kind of adjustable overturning-preventing thermal insulation test tube frame for intelligent medical detection |
| KR101977628B1 (en) * | 2017-09-29 | 2019-05-13 | (주)로봇앤드디자인 | Holder structure for flask and vortex apparatus |
| CN107930493A (en) * | 2017-12-09 | 2018-04-20 | 北京中飞华正检测技术服务有限公司 | A kind of water-bath constant temperature oscillator |
| CN210646524U (en) * | 2019-09-02 | 2020-06-02 | 中国人民解放军第四军医大学第一附属医院 | Clinical laboratory is with device of submitting to examine of drawing blood |
| CN113171710B (en) * | 2020-03-19 | 2022-07-05 | 韩立志 | Clinical laboratory detects with shaking even test-tube rack automatically |
| CN111774119A (en) * | 2020-06-28 | 2020-10-16 | 王丽娟 | Pathology department uses human sampling device |
| CN112093216A (en) * | 2020-08-27 | 2020-12-18 | 新疆卡纳思生物技术有限公司 | 5' -nucleotidase determination kit with shaking-up function |
| CN213611098U (en) * | 2020-10-23 | 2021-07-06 | 攀枝花市中西医结合医院 | Even device is shaken with shaking to clinical laboratory blood of hospital |
| CN214288340U (en) * | 2020-11-13 | 2021-09-28 | 吴佳莺 | Clinical laboratory uses test tube transport case |
| CN112871039B (en) * | 2021-03-01 | 2022-07-15 | 四川大学华西医院 | Heparin tube mixing device |
| CN113101998A (en) * | 2021-04-21 | 2021-07-13 | 武汉优思创贸易有限公司 | Instrument of taking in batches of clinical laboratory's test tube of hospital |
| CN113522117B (en) * | 2021-07-13 | 2022-07-26 | 知弗(上海)科技股份有限公司 | Be used for macromolecular chemical material composition analytical equipment |
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