CN114113266A - Sensing device for analyzing sweat marker - Google Patents
Sensing device for analyzing sweat marker Download PDFInfo
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- CN114113266A CN114113266A CN202111562737.9A CN202111562737A CN114113266A CN 114113266 A CN114113266 A CN 114113266A CN 202111562737 A CN202111562737 A CN 202111562737A CN 114113266 A CN114113266 A CN 114113266A
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Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
Abstract
The invention belongs to the technical field of biosensing, and particularly relates to a sensing device for analyzing a sweat marker, which comprises sweat collecting patches with different hydrophilicity and hydrophobicity, a sweat discharging micro-channel, an electrochemical electrode, a data acquisition and processing module and a terminal display screen, wherein the electrochemical electrode is connected with the data acquisition and processing module through an electrode connecting wire, and a Bluetooth device in the data acquisition and processing module transmits data to a terminal display; the sweat collecting patch is composed of a plurality of fabrics with different hydrophilic and hydrophobic properties, the number of the sweat micro-channels is a plurality, and the electrochemical electrode comprises a working electrode and a reference electrode; the beneficial effects are as follows: the fabric layer of the sweat collecting patch is formed by combining hydrophilic layer materials with different gradients, the peripheral hydrophobic layer can effectively prevent sweat outside the area from entering the detection area, and the fidelity of the sensor in sweat detection of a specific part can be improved.
Description
Technical Field
The invention belongs to the technical field of biosensing, and particularly relates to a sensing device for analyzing sweat markers.
Background
In recent years, the development of transcutaneous biosensors has received a great deal of attention due to the miniaturization of conventional sensing mechanisms. Microneedle biosensors have achieved satisfactory results in detecting glucose and selecting biomarkers in peripheral blood and interstitial fluid, but these devices acquire analytes in a minimally invasive manner because they pierce the skin on the order of microns, which in some cases can cause discomfort or infection to the user, with potential risk. Therefore, wearable portable biosensors for detecting and monitoring sweat analytes, which instantly detect the physical health status of an individual in an inexpensive and non-invasive manner, are a better choice.
The sweat of the human body contains rich physiological information, and noninvasive detection can be realized for obtaining samples. In the process of exercise, sweat gland of various parts of a human body secretes sweat due to heat, the loss of sodium ions and potassium ions in the sweat can cause hyponatremia, hypokalemia, muscle spasm, dehydration and the like, chloride ions can be used as markers of cystic fibrosis, hydration state and electrolyte storage, glucose can reflect blood sugar level, lactic acid can be used as a sensitive marker of pressure ischemia, and cortisol can be used as an index for evaluating an immune system and can also be used for diagnosing cushing's syndrome and addison's disease. Therefore, the detection of specific markers in sweat has a very important role for the assessment of the health status of the body. However, most current sweat sensors have errors in analyzing sweat markers at specific positions, particularly errors from outside sweat collection points, such as peripheral sweat entering a working electrode by a water absorption pad and influence on new sweat detection caused by old sweat not to be eliminated.
Disclosure of Invention
The invention aims to provide a sensing device for analyzing a sweat marker, aiming at overcoming the defects in the prior art, and solving the problem that peripheral sweat has influence on sweat at a specific part in the sweat analyte detection process.
In order to achieve the purpose, the invention adopts the technical scheme that:
a sensing device for sweat marker analysis, characterized by: the sweat collection patch with different hydrophilicity and hydrophobicity, the sweat collection microchannel 5, the electrochemical electrode 6, the data acquisition and processing module and the terminal display screen are included, the electrochemical electrode 6 is connected with the data acquisition and processing module through an electrode connecting wire 7, and a Bluetooth device in the data acquisition and processing module transmits data to a terminal display; the sweat collecting patch is composed of a plurality of fabrics with different hydrophilic and hydrophobic properties, the number of the sweat micro-channels 5 is a plurality, and the electrochemical electrode 6 comprises a working electrode and a reference electrode.
The sweat collection patch is circular or elliptical in shape, the hydrophilicity of the sweat collection patch is gradually enhanced from the edge to the center, and the number of hydrophilic and hydrophobic areas is more than or equal to 3.
The sweat collecting patch is sequentially provided with a hydrophobic area, a mild hydrophilic area, a moderate hydrophilic area and a super hydrophilic area from the edge to the center, the width from the hydrophobic area to the super hydrophilic area is sequentially increased, and one or more of the mild hydrophilic area and the moderate hydrophilic area can be used.
The sweat collecting patch is characterized in that the width of a hydrophobic area of the sweat collecting patch is 0.5-1mm, the width of a mild hydrophilic area of the sweat collecting patch is 1-2mm, the width of a moderate hydrophilic area of the sweat collecting patch is 2-3mm, the radius of a super hydrophilic area of the sweat collecting patch is 3-4mm, and finally a circular patch with the radius of 6.5-10mm is formed for sweat collection.
The sweat collecting patch is of a multilayer structure and sequentially comprises a sweat collecting fabric layer 8, a waterproof layer 9, a first PET substrate 10, a microfluidic layer 11, a second PET substrate 12 and an insulating layer 13 from bottom to top; the collection fabric layer 8 can be woven by natural or artificial fibers with different hydrophilicity and hydrophobicity, and the surface of the hydrophilic and hydrophobic part can be modified to enhance the hydrophilicity or hydrophobicity of each part, so that sweat can be collected; the waterproof layer 9 is covered on the surface of the patch outside the super-hydrophilic area in advance; and a plurality of perspiration micro-channels 5 are distributed on the circumference of the microfluidic layer 11.
The number of the perspiration micro-channels 5 is 4-16, and the width of each perspiration micro-channel 5 is 2-0.5 mm. A number of said perspiration micro channels 5 enable the detected sweat to be removed in time through the micro channels in order to allow the replacement of old and new sweat in time.
The electrochemical electrode 6 is attached to the super-hydrophilic region of the sweat collection patch, and the electrochemical electrode 6 can be a working electrode for detecting sodium ions, potassium ions or the like, and can also be a counter/reference electrode.
The hydrophilic and hydrophobic regions, the perspiration micro-channels 5 and the electrochemical electrodes 6 on the sweat collection patch may be combined in any number.
The electrochemical electrode 6 is capable of detecting electrolytes, which may be sodium, potassium, calcium and chloride ions, and metabolites, which may be glucose, lactate and cortisol, in sweat.
Because the sweat collecting patch has hydrophilicity with different gradients, when the sweat is collected, the sweat can be conveyed to the super-hydrophilic area in a one-way mode to be detected by the sensor, and the hydrophobic area effectively prevents the sweat around the patch from entering the area to be detected, so that the interference of the sweat outside the detection area on the sweat of the target area can be effectively reduced.
Because sweat constantly gathers in super hydrophilic district to the pressure that so that this region is constantly increased to in the middle of squeezing the sweat that has detected into the microchannel, then separate it from the sensor through the pipeline, be convenient for the timely detection of new sweat.
Compared with the defects and shortcomings of the prior art, the invention has the following beneficial effects:
1. the fabric layer of the sweat collecting patch is formed by combining hydrophilic layer materials with different gradients, the peripheral hydrophobic layer can effectively prevent sweat outside the area from entering the detection area, and the fidelity of the sensor in sweat detection of a specific part can be improved.
2. According to the sweat collection patch, the microfluid channel is additionally arranged on the patch outside the super-hydrophilic region, so that detected sweat can be quickly discharged out of the device, the sensor has good selectivity on detection of new sweat, and interference of old sweat on detection of a new sweat marker is reduced.
3. The number of hydrophilic and hydrophobic areas and the number of micro-channel pipelines can realize various combinations, and a more flexible mode is realized for the preparation of the patch.
Drawings
For a more clear understanding of the present invention, the present disclosure will be further described by reference to the drawings and illustrative embodiments which are provided for illustration and are not to be construed as limiting the disclosure.
FIG. 1 is a schematic diagram of the construction of a sweat sensor patch of the present invention;
FIG. 2 is an exploded view of the sweat collection patch of the present invention;
fig. 3 is a schematic diagram of a sweat detection device of the present invention.
Shown in the figure: the water repellent area comprises a hydrophobic area 1, a mild hydrophilic area 2, a moderate hydrophilic area 3, a super-hydrophilic area 4, a perspiration micro-channel 5, an electrochemical electrode 6, an electrode connecting wire 7, a hydrophilic-hydrophobic fabric layer 8, a waterproof layer 9, a first PET substrate 10, a microfluidic layer 11, a second PET substrate 12 and an insulating layer 13.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
A sensing device for analyzing sweat markers is shown in figure 1 and comprises sweat collecting patches with different hydrophilicity and hydrophobicity, a sweat discharging micro-channel 5, an electrochemical electrode 6, a data acquisition and processing module and a terminal display screen, wherein the electrochemical electrode 6 is connected with the data acquisition and processing module through an electrode connecting wire 7, and a Bluetooth device in the data acquisition and processing module transmits data to a terminal display; the sweat collecting patch is composed of a plurality of fabrics with different hydrophilic and hydrophobic properties, the number of the sweat micro-channels 5 is a plurality, and the electrochemical electrode 6 comprises a working electrode and a reference electrode.
The sweat collection patch is circular or elliptical in shape, the hydrophilicity of the sweat collection patch is gradually enhanced from the edge to the center, and the number of hydrophilic and hydrophobic areas is more than or equal to 3. The sweat collecting patch is sequentially provided with a hydrophobic area, a mild hydrophilic area, a moderate hydrophilic area and a super hydrophilic area from the edge to the center, the width from the hydrophobic area to the super hydrophilic area is sequentially increased, and one or more of the mild hydrophilic area and the moderate hydrophilic area can be used. The sweat collecting patch is characterized in that the width of a hydrophobic area of the sweat collecting patch is 0.5-1mm, the width of a mild hydrophilic area of the sweat collecting patch is 1-2mm, the width of a moderate hydrophilic area of the sweat collecting patch is 2-3mm, the radius of a super hydrophilic area of the sweat collecting patch is 3-4mm, and finally a circular patch with the radius of 6.5-10mm is formed for sweat collection.
The paster is collected to sweat is multilayer structure, and from the bottom up comprises sweat collection fabric layer 8, waterproof layer 9, first PET substrate 10, micro-fluidic layer 11, second PET substrate 12 and insulating layer 13 in proper order, collect fabric layer 8 and can weave through the natural or artificial fiber who has different hydrophilicity and hydrophobicity, also can be through modifying the position surface of hydrophilicity and hydrophobicity, strengthen the hydrophilicity or the hydrophobic ability of each position to the realization is to the collection of accomodating of sweat. The waterproof layer 9 is covered on the surface of the patch outside the super-hydrophilic area in advance; and a plurality of perspiration micro-channels 5 are distributed on the circumference of the microfluidic layer 11. The number of the perspiration micro-channels 5 is 4-16, and the width of each perspiration micro-channel 5 is 2-0.5 mm. A number of said perspiration micro channels 5 enable the detected sweat to be removed in time through the micro channels in order to allow the replacement of old and new sweat in time. The electrochemical electrode 6 is attached to the super-hydrophilic region of the sweat collection patch, and the electrochemical electrode 6 can be a working electrode for detecting sodium ions, potassium ions or the like, and can also be a counter/reference electrode. The hydrophilic and hydrophobic regions, the perspiration micro-channels 5 and the electrochemical electrodes 6 on the sweat collection patch may be combined in any number. The electrochemical electrode 6 is capable of detecting electrolytes, which may be sodium, potassium, calcium and chloride ions, and metabolites, which may be glucose, lactate and cortisol, in sweat.
In some embodiments of the invention, the hydrophilic and hydrophobic regions of the sweat collection patch are not limited to 4, and may be greater or less than 4.
In some embodiments of the invention, the number of said perspiration micro-channels 5 is 4-16, and may be 4, 8, 12, 16.
In some embodiments of the invention, the layout of the perspiration micro-channels 5 is not limited to the rectilinear type, but also other shapes exist, such as the Y-shape.
In some embodiments of the present invention, the number of the electrochemical electrodes 6 is not limited to 5, and can be adjusted accordingly according to actual requirements, and the electrodes include a working electrode and a counter/reference electrode.
The principle of the invention is as follows:
the patch is collected to complete sweat includes hydrophilic-hydrophobic fabric layer from bottom to top, the waterproof layer, first PET substrate, the micro-fluidic layer, second PET substrate and insulating layer, a sweat that is used for specific site collects the patch, including hydrophobic district 1, slight hydrophilic district 2, moderate hydrophilic district 3 and super hydrophilic district 4, link together the fabric of different hydrophilicities through plasma etching, form a circular fabric layer, outmost hydrophobic district, be used for preventing the outer sweat of patch to get into and treat the district, by outer to interior, hydrophilicity strengthens gradually, and the width in each district increases in proper order, such gradient distribution has the sweat that does benefit to in the patch to gather from hydrophobic district spontaneous super hydrophilic district, thereby realize electrochemical electrode's short-term test.
The sweat that super hydrophilic district gathering is used for the real-time supervision of sensor, but the human body is at the continuous motion in-process, can constantly produce new sweat, in order to guarantee that the sensor electrode can be to the sweat work of new production, consequently need install several, the perspire microchannel on the paster, the old sweat of being convenient for in time discharges the detection zone. The perspiration micro-channel may be prepared according to the prior art, such as soft lithography and laser ablation techniques. And finally, assembling the prepared sweat discharging micro-channel and the sweat collecting patch into a complete new patch through plasma etching. Note that the combination of the number of hydrophilic and hydrophobic regions of the patch and the number of microchannel tubes may be arbitrarily combined without being bound by specific conditions.
The prepared electrochemical electrode is placed in the super-hydrophilic area of the patch, so that sweat can be fully contacted with the detection electrode, and an electrode connecting wire connected with the electrochemical electrode is not overlapped with the microchannel, so that the microchannel is better protected from being damaged. In the detection process, the electrode connecting wire is connected to the data processing module, converts the acquired electric signals into digital signals, and then transmits the digital signals to the terminal display through the Bluetooth module. The method provides a better choice for the accurate analysis of the sweat at a specific part, improves the accuracy of the analysis of the sweat marker, and has better application prospect.
Example 1
As shown in fig. 1, the sweat collection patch consists of 4 fabrics with different hydrophilic and hydrophobic areas, 8 micro-channels for sweat removal and 5 electrochemical electrodes, wherein the fabrics comprise a hydrophobic area 1, a slightly hydrophilic area 2, a medium hydrophilic area 3 and a super hydrophilic area 4, and the hydrophobic area 1, the slightly hydrophilic area 2, the medium hydrophilic area 3 and the super hydrophilic area 4 are sequentially integrated through plasma etching, the width of the hydrophobic area is 0.5mm, the width of the slightly hydrophilic area is 1mm, the width of the medium hydrophilic area is 2mm, the width of the super hydrophilic area is 3mm, and finally, a circular patch with the radius of 6.5mm is formed. 8 micro-channels were prepared by soft lithography, each channel having a length of 2.5mm and a width of 1mm, and an exploded view of the entire patch is shown in FIG. 2. The electrochemical electrode is composed of a sodium ion electrode, a potassium ion electrode and a glucose electrode which are used as working electrodes, a reference electrode shared by the sodium ion and the potassium ion electrode and a glucose reference electrode, the preparation of each electrode can be carried out according to the prior technical scheme, the surface of the electrode is modified by using a specific reagent before measurement, for example, the sodium ion electrode is soaked in a sodium chloride solution with a certain concentration one hour before the measurement so as to reduce the potential drift generated in the test process. As shown in the schematic diagram of the sweat detection device in fig. 3, the electrode connection line connects the electrochemical electrode and the data collecting and processing module together for data collection and analysis, and transmits the analyzed data to the terminal display through the bluetooth device in the module.
Example 2
The sweat is collected the paster and is become by 4 fabric pasters in different hydrophilic and hydrophobic district, 4 microchannels and 5 electrochemical electrode that are used for perspiring, the fabric paster includes hydrophobic district 1, mild hydrophilic district 2, moderate hydrophilic district 3 and super hydrophilic district 4, integrates it according to the order through plasma etching, the hydrophobic district width of this embodiment is 0.75mm, mild hydrophilic district width is 1.5mm, moderate hydrophilic district width is 2.5mm, super hydrophilic district radius is 3.5mm, finally constitute a radius and be 8.25 mm's circular paster. 4 microchannels were prepared by soft lithography, each channel 4.75mm long and 2mm wide. The connection mode of the electrochemical electrode and the subsequent lead and the acquisition of detection data are the same as those in example 1, and are not described again.
Example 3
The sweat is collected the paster and is become by 4 fabrics in different hydrophilic and hydrophobic district, 4 microchannels and 5 electrochemical electrode that are used for perspiring, the fabric includes hydrophobic district 1, mild hydrophilic district 2, moderate hydrophilic district 3 and super hydrophilic district 4, integrates it in order through plasma etching, the hydrophobic district width of this embodiment is 1mm, mild hydrophilic district width is 2mm, moderate hydrophilic district width is 3mm, super hydrophilic district radius is 4mm, finally constitute a radius and be 10 mm's circular paster. 12 microchannels, each 6mm long and 1mm wide, were prepared by soft lithography. The connection mode of the electrochemical electrode and the subsequent lead and the acquisition of detection data are the same as those in example 1, and are not described again.
Example 4
The sweat is collected the paster and is become by 4 fabrics in different hydrophilic and hydrophobic district, 4 microchannels and five electrochemical electrode that are used for perspiring, and the fabric includes hydrophobic district 1, mild hydrophilic district 2, moderate hydrophilic district 3 and super hydrophilic district 4, integrates it in order through plasma etching, and the hydrophobic district width of this embodiment is 1mm, and mild hydrophilic district width is 2mm, and moderate hydrophilic district width is 3mm, and super hydrophilic district radius is 4mm, finally constitutes a radius and is 10 mm's circular paster. 16 microchannels were prepared by soft lithography, each channel 6mm long and 0.75mm wide. The connection mode of the electrochemical electrode and the subsequent lead and the acquisition of detection data are the same as those in example 1, and are not described again.
Example 5
The sweat collecting patch consists of 3 fabrics in different hydrophilic and hydrophobic areas, 8 micro-channels for sweat discharging and five electrochemical electrodes, wherein the fabrics comprise a hydrophobic area, a middle hydrophilic area and a super hydrophilic area, the hydrophobic area, the middle hydrophilic area and the super hydrophilic area are sequentially integrated through plasma etching, the width of the hydrophobic area is 1mm, the width of the middle hydrophilic area is 3mm, the radius of the super hydrophilic area is 5mm, and finally, a circular patch with the radius of 9mm is formed. 12 microchannels, each 6mm long and 1mm wide, were prepared by soft lithography. The connection mode of the electrochemical electrode and the subsequent lead and the acquisition of detection data are the same as those in example 1, and are not described again.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A sensing device for sweat marker analysis, characterized by: the sweat collection patch with different hydrophilicity and hydrophobicity, the sweat collection micro-channel (5), the electrochemical electrode (6), the data collection processing module and the terminal display screen are included, the electrochemical electrode (6) is connected with the data collection processing module through an electrode connecting wire (7), and a Bluetooth device in the data collection processing module transmits data to the terminal display; the sweat collection patch is composed of a plurality of fabrics with different hydrophilic and hydrophobic properties, the number of the sweat micro-channels (5) is several, and the electrochemical electrode (6) comprises a working electrode and a reference electrode.
2. A sensing device for sweat marker analysis according to claim 1, wherein: the sweat collection patch is circular or elliptical in shape, the hydrophilicity of the sweat collection patch is gradually enhanced from the edge to the center, and the number of hydrophilic and hydrophobic areas is more than or equal to 3.
3. A sensing device for sweat marker analysis according to claim 2, wherein: the sweat collecting patch is sequentially provided with a hydrophobic area, a mild hydrophilic area, a moderate hydrophilic area and a super hydrophilic area from the edge to the center, the width of the hydrophobic area to the super hydrophilic area is sequentially increased, and one or more of the mild hydrophilic area and the moderate hydrophilic area can be used.
4. A sensing device for sweat marker analysis according to claim 3, wherein: the sweat collecting patch is characterized in that the width of a hydrophobic area of the sweat collecting patch is 0.5-1mm, the width of a mild hydrophilic area of the sweat collecting patch is 1-2mm, the width of a moderate hydrophilic area of the sweat collecting patch is 2-3mm, the radius of a super hydrophilic area of the sweat collecting patch is 3-4mm, and finally a circular patch with the radius of 6.5-10mm is formed for sweat collection.
5. A sensing device for sweat marker analysis according to claim 1, wherein: the sweat collecting patch is of a multilayer structure and sequentially comprises a sweat collecting fabric layer (8), a waterproof layer (9), a first PET (polyethylene terephthalate) substrate (10), a microfluidic layer (11), a second PET substrate (12) and an insulating layer (13) from bottom to top; the collecting fabric layer (8) can be woven by natural or artificial fibers with different hydrophilicity and hydrophobicity, and the surface of the hydrophilic and hydrophobic part can be modified to enhance the hydrophilicity or hydrophobicity of each part, so that sweat can be collected; the waterproof layer (9) covers the surface of the patch outside the super-hydrophilic area in advance; a plurality of perspiration micro-channels (5) are distributed on the circumference of the micro-fluidic layer (11).
6. A sensing device for sweat marker analysis according to claim 5, wherein: the number of the perspiration micro-channels (5) is 4-16, and the width of each perspiration micro-channel (5) is 2-0.5 mm.
7. A sensing device for sweat marker analysis according to claim 3 or 4, wherein: the electrochemical electrode (6) is attached to the super-hydrophilic region of the sweat collection patch, and the electrochemical electrode (6) can be a working electrode for detecting sodium ions, potassium ions and the like and can also be a counter/reference electrode.
8. A sensing device for sweat marker analysis according to claims 1-7 wherein: the hydrophilic and hydrophobic regions, the perspiration micro-channels (5) and the electrochemical electrodes (6) on the sweat collection patch can be combined in any number.
9. A sensing device for sweat marker analysis according to claims 1-7 wherein: the electrochemical electrode (6) is capable of detecting electrolytes, which may be sodium, potassium, calcium and chloride ions, and metabolites, which may be glucose, lactate and cortisol, in sweat.
Priority Applications (1)
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