CN114101318A - Treatment method of petroleum-polluted soil - Google Patents
Treatment method of petroleum-polluted soil Download PDFInfo
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- CN114101318A CN114101318A CN202010872919.5A CN202010872919A CN114101318A CN 114101318 A CN114101318 A CN 114101318A CN 202010872919 A CN202010872919 A CN 202010872919A CN 114101318 A CN114101318 A CN 114101318A
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention provides a method for treating petroleum-polluted soil. The treatment method of the petroleum-polluted soil comprises the following steps: step S1, adding a microbial agent into the petroleum-polluted soil; step S2, planting plants on the petroleum polluted soil; and step S3, spraying a surfactant solution to the petroleum-polluted soil. Under the condition of using the treatment method of the petroleum-polluted soil, the degradation efficiency of petroleum dirt in the soil is effectively improved, and the technical effect of greatly shortening the operation time for treating the petroleum-polluted soil is achieved.
Description
Technical Field
The invention relates to a treatment technology of petroleum-polluted soil, in particular to a treatment method of petroleum-polluted soil.
Background
The problem of petroleum pollution to nearby soil generally exists in the oil field exploitation process. The disposal of petroleum contaminated soils has become a concern in recent years due to petroleum contamination from leakage or drainage problems that can occur during the exploration, transportation, handling, processing and use of petroleum. The petroleum polluted soil treatment methods are various, and the bioremediation technology becomes a current research hotspot due to the characteristics of low cost, small environmental influence, no secondary pollution, no damage to the soil structure and the like. Bioremediation techniques include microbial, phytoremediation, animal remediation and microbial joint remediation techniques, common microbial joint remediation being plant-microbial, electrokinetic-microbial and oxidative-microbial. A large number of researches show that the microorganism combined repair technology has better repair effect and efficiency than the single biological repair technology. The existing bioremediation technology, especially the microbial remediation technology, has high requirements on temperature, humidity, oxygen, nutrition and the like, so that the management difficulty in the remediation process is high. The bioremediation technology used in the prior art still has the problems of long service cycle, poor efficiency and the like.
Disclosure of Invention
The invention mainly aims to provide a method for treating petroleum-polluted soil, which aims to solve the problems of long service cycle and poor efficiency of a bioremediation technology in the prior art.
In order to achieve the above object, according to one aspect of the present invention, there is provided a method for treating oil-contaminated soil, comprising: step S1, adding a microbial agent into the petroleum-polluted soil; step S2, planting plants on the petroleum polluted soil; and step S3, spraying a surfactant solution to the petroleum-polluted soil.
Further, in the above method, the microbial agent includes a microbial liquid and a carrier.
Further, in the above method, the microbial liquid comprises Pseudomonas aeruginosa (Pseudomonas aeruginosa), Bacillus subtilis (Bacillus subtilis)Any one or more of Bacillus subtilis and Aspergillus versicolor; preferably, the microbial liquid comprises a liquid of pseudomonas aeruginosa, a liquid of bacillus subtilis and a liquid of aspergillus versicolor; more preferably, the volume ratio of the pseudomonas aeruginosa bacterial liquid, the bacillus subtilis bacterial liquid and the aspergillus versicolor bacterial liquid is 1-2: 1-1.5: 0.5-1, and the strain concentrations in the pseudomonas aeruginosa bacterial liquid, the bacillus subtilis bacterial liquid and the aspergillus versicolor bacterial liquid are respectively and independently more than or equal to 1 x 109cfu/ml。
Further, in the above method, the amount of the microbial cell broth is 10mL to 20mL per 1kg of the vector, and it is preferable that the strain concentrations in the bacterial broth of Pseudomonas aeruginosa, the bacterial broth of Bacillus subtilis and the bacterial broth of Aspergillus versicolor are each independently 1X 109cfu/ml to 6X 109In the cfu/ml range.
Further, in the above method, the carrier comprises any one or a combination of more of cotton seed hulls, rice hulls, bran, corn cobs, and activated carbon; preferably, the carrier comprises cottonseed hulls and rice hulls; further preferably, the weight ratio of the cottonseed hulls to the rice hulls is in the range of 1: 1-2.
Further, in the above method, the amount of the microbial agent is 2 to 4 wt% based on the total weight of the petroleum-contaminated soil.
Further, in the above method, step S1 further includes adding a soil conditioner to the petroleum-contaminated soil, preferably in an amount of 0.5 wt% to 1 wt% based on the total weight of the petroleum-contaminated soil, preferably in the soil conditioner, nitrogen: phosphorus: the weight ratio of potassium is 17-22: 0.8-1.2: 1.3-1.6; preferably, the soil amendment comprises a combination of any of urea, ammonium bicarbonate, diammonium phosphate, calcium superphosphate, dipotassium phosphate trihydrate, potassium chloride, and potassium humate; further preferably, the soil conditioner comprises 78-83 wt% of urea, 8-13 wt% of diammonium hydrogen phosphate and 9-14 wt% of potassium humate.
Further, in the above method, the plant comprises any one or more of alfalfa, cotton, haloxylon ammodendron and reed in combination, preferably, the plant is reed.
Further, in the above method, the plant is planted at a density of 30 plants/m2To 50 strains/m2。
Further, in the above method, the surfactant solution includes rhamnolipid, sophorolipid, lipopeptide, natural soap powder, etc.; preferably, the surfactant is a rhamnolipid; further preferably, the addition amount of rhamnolipid per kilogram of petroleum-contaminated soil is 60-120 mg; further preferably, the rhamnolipid is prepared into a solution with the mass concentration of 0.5 percent and then sprayed to the petroleum-polluted soil.
Further, in the above method, before step S1, the processing method further includes crushing the oil-contaminated soil so that the particle size of the oil-contaminated soil is 10mm or less.
Further, in the above method, between the step S1 and the step S2, the treating method further comprises stacking the petroleum-contaminated soil at a thickness of 50cm to 70cm, preferably on the site of the impermeable treatment.
Further, in the above method, the humidity of the petroleum-contaminated soil is in the range of 15% to 20%.
Further, in the above method, the treatment method is performed at a temperature ranging from 10 ℃ to 40 ℃, and preferably the treatment method further comprises repeating step S3 every 30 days to 40 days.
Under the condition of using the treatment method of the petroleum-polluted soil, the problems in the prior art are overcome, the degradation efficiency of petroleum pollutants in the soil is effectively improved, and the technical effect of greatly shortening the operation time for treating the petroleum-polluted soil is achieved.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 shows the results of example 1, comparative examples 1 to 3 of the treatment method of petroleum-contaminated soil according to the present invention;
FIG. 2 shows the results of example 2, comparative examples 4 to 6 of the treatment method of petroleum-contaminated soil according to the present invention; and
FIG. 3 shows the results of example 3 and comparative examples 7 to 9 of the method for treating petroleum-contaminated soil according to the present invention.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
As can be understood from the foregoing background, the methods in the prior art still have the problems of long repair cycle and poor efficiency. Therefore, in order to solve the problem, the present application provides a method for treating petroleum-contaminated soil, comprising the steps of S1, adding a microbial agent to the petroleum-contaminated soil; step S2, planting plants on the petroleum polluted soil; and step S3, spraying a surfactant solution to the petroleum-polluted soil.
Unlike prior art methods of treating petroleum contaminated soil that use only plants, microorganisms, and combinations of plants and microorganisms. In the present application, a combined treatment method of microorganisms, plants and surfactants is used. In step S1, a microbial inoculum is added to the contaminated soil and mixed thoroughly with the soil. In step S2, plants are planted on the soil mixed with the microbial inoculum, wherein the plants that can be used include any plants that are effective in remediating petroleum-contaminated soil. In step S3, the petroleum-contaminated soil is sprayed with a surfactant solution so that the surfactant is uniformly distributed in the soil. When the treatment method is used, the petroleum-polluted soil is sprayed by the surfactant, so that the petroleum pollutants are separated from the combined state of the petroleum pollutants and the soil, the contact between the petroleum pollutants and the microorganisms and the root systems of plants is increased, and the petroleum pollutants can be degraded quickly and effectively. In addition, plants are planted on the contaminated soil, and while the plants absorb and purify petroleum pollutants in the soil, since the roots and stems thereof are deep into the soil, oxygen can be continuously supplied to aerobic microorganisms in the soil to further increase the degradation efficiency of the petroleum pollutants. Further, the applicant of the present application found that the number of the microorganism-degrading bacteria in the soil is effectively increased and the activity of the microorganism-degrading bacteria is enhanced after the contaminated soil is sprayed with the surfactant.
In conclusion, by using the method for treating the petroleum-polluted soil, the degradation efficiency of the petroleum dirt in the soil can be effectively improved, and the operation time for treating the petroleum-polluted soil is greatly shortened.
In some embodiments of the present application, the microbial agent consists of a microbial fluid and a carrier. In the present application, the microbial agent used includes a microbial solution and a carrier for carrying the microbial solution. In some embodiments, the microbial solution used comprises a combination of any one or more of Pseudomonas aeruginosa (Pseudomonas aeruginosa), Bacillus subtilis (Bacillus subtilis), Aspergillus versicolor (Aspergillus versicolor). In the case of using any one of the above microorganisms alone, the petroleum pollutants can be rapidly degraded into harmless substances. In the case where two or more microorganisms are used simultaneously, for example, a combination of Pseudomonas aeruginosa and Bacillus subtilis, a combination of Bacillus subtilis and Aspergillus versicolor, a combination of Pseudomonas aeruginosa and Aspergillus versicolor, and the use of all microorganisms at the same time, the microorganisms will mutually promote and form a combined community of microorganisms. In the process of degrading petroleum pollutants by the microbial union community, the intermediate products of one or more microorganisms usually act as catalytic enzymes of another one or more microorganisms, so that the microorganisms mutually promote growth to accelerate aerobic decomposition of the petroleum pollutants. In a preferred embodiment, the microbial liquid comprises a liquid of pseudomonas aeruginosa, a liquid of bacillus subtilis and a liquid of aspergillus versicolor, and the volume ratio of the liquid of pseudomonas aeruginosa, the liquid of bacillus subtilis and the liquid of aspergillus versicolor is within the range of 1-2: 1-1.5: 0.5-1.
In a further embodiment, the amount of the microbial broth is 10mL to 20mL per 1kg of the vector. When the amount of the microbial cell culture solution used in the carrier is in the range of 10mL/1kg of carrier to 20mL/1kg of carrier, a more desirable amount of microbial population can be produced in the soil. When the amount of the microbial inoculum is less than 10mL/1kg of the carrier, the amount of the microbes is insufficient, so that the efficient effect of degrading the petroleum pollutants cannot be realized, and when the amount of the microbial inoculum is more than 20mL/1kg of the carrier, the amount of the microbial flora in the soil is excessive, so that the flora cannot obtain sufficient oxygen and nutrition, and the degradation effect of the petroleum pollutants is disadvantageously reduced. In addition, the microorganisms in the carrier are too few, the oil removal effect is not obvious, and when the microorganisms in the biochar are excessive, the competition of the microorganisms on oxygen and nutrient substances is increased, and the growth of the strain is hindered.
In a further embodiment, the concentration of the strain in the bacterial solution of Pseudomonas aeruginosa, the bacterial solution of Bacillus subtilis and the bacterial solution of Aspergillus versicolor is 1X 10 or more independently9cfu/ml. In a preferred embodiment, the strain concentration in the bacterial solution of Pseudomonas aeruginosa, the bacterial solution of Bacillus subtilis and the bacterial solution of Aspergillus versicolor is 1X 10 independently9cfu/ml to 6X 109In the cfu/ml range.
In some embodiments of the present application, the carrier comprises any one or a combination of more of cottonseed hulls, rice hulls, bran, corn cobs, and activated carbon. In the microbial agent of the present application, the microbial liquid is usually supported on a carrier, wherein the carrier may comprise any substance which can support the microbial liquid and does not cause secondary pollution to soil, and preferably, the carrier comprises cotton seed hulls and rice hulls. After the microbial agent enters the soil, the carrier will rapidly decompose to release the nutrients necessary for microbial growth, thereby rapidly establishing microbial colonies in the soil. In a preferred embodiment, the carrier comprises cottonseed hulls and rice hulls in a weight ratio of cottonseed hulls to rice hulls in the range of 1:1 to 2.
In some embodiments of the present application, the amount of microbial agent is 2 to 4 wt% based on the total weight of the petroleum-contaminated soil. The amount of microbial agent should be maintained in the range of 2 wt% to 4 wt% relative to the total weight of the petroleum-contaminated soil. When the amount of the microbial agent is less than 2 wt%, petroleum pollutants cannot be effectively degraded; and when the amount of the microbial agent is more than 4 wt%, unnecessary waste may be caused.
In a further embodiment of the present application, step S1 further comprises adding a soil conditioner to the petroleum-contaminated soil. In the process of adding the microbial agent into the petroleum-polluted soil, a soil conditioner can be further added, so that the quality of the soil is improved while the petroleum pollutants in the soil are degraded.
In a further embodiment of the present application, the amount of the soil amendment is 0.5 to 1 wt% based on the total weight of the petroleum-contaminated soil. Within the scope of the soil conditioner of the present application, the soil conditioner can improve the physical properties of soil and increase the nutrients of soil. When the amount of the soil conditioner is less than 0.5 wt%, it is insufficient to provide effective nutritional supplement to the soil; and when the amount of the soil conditioner is more than 1 wt%, the excessive soil conditioner adversely affects the growth of microorganisms in the microbial agent, thereby reducing the efficiency of treating the petroleum pollutants.
In a further embodiment, in the soil amendment, the ratio of nitrogen: phosphorus: the weight-to-weight ratio of potassium is 17-22: 0.8-1.2: 1.3 to 1.6. Within the above range, the soil conditioner can effectively supply nitrogen, phosphorus and potassium elements necessary for the growth of plants to the soil, so that the plants planted in step S2 can grow to achieve the effect of improving the soil quality. In a preferred embodiment, the soil amendment comprises any one or combination of urea, ammonium bicarbonate, diammonium phosphate, calcium superphosphate, dipotassium phosphate trihydrate, potassium chloride, and potassium humate. Further preferably, the soil conditioner comprises 78 wt% to 83 wt% urea, 8 wt% to 13 wt% diammonium phosphate and 9 wt% to 14 wt% potassium humate.
In a further embodiment, the plant comprises any one or a combination of alfalfa, cotton, haloxylon and reed, provided that it is capable of growing normally in saline and alkaline soils. The plants planted in step S2 of the present application may include any plants capable of remediating petroleum-contaminated soil. In a preferred embodiment, the plant used herein is reed. Because the plant that uses in this application is the herbaceous plant, consequently growth that can be quick to can realize the restoration to oil contaminated soil fast. Furthermore, as the plant used in this application grows faster, the roots of the plant will also grow faster, thus providing favourable conditions for growth without microorganisms.
In a further embodiment, the plants are planted at a density of 30 plants/m2To 50 strains/m2. In the present application, the plants should be planted at a density of 30 plants/m2To 50 strains/m2Within the range of (1). When the planting density is lower than 30 plants/m2In the case where the amount of the plant is too low, the soil purification ability of the plant is insufficient, and the oxygen supply ability of the plant rhizome to microorganisms in the soil is insufficient. When the planting density is higher than 50 plants/m2When the amount of plants is too high, the plants and microorganisms compete for nutrients in the soil, disadvantageously resulting in the death of microorganisms in the soil, thereby reducing the soil restoration ability.
In some embodiments of the present application, the surfactant solution includes rhamnolipids, sophorolipids, lipopeptides, natural soap powder, and the like, provided that the surfactant used is a biodegradable surfactant or an environmentally friendly surfactant. In a preferred embodiment, the surfactant used herein is a rhamnolipid. In a further preferred embodiment, the addition amount of the rhamnolipid per kilogram of the petroleum-contaminated soil is 60-120 mg. In step S3, rhamnolipids are first mixed with water to prepare a surfactant solution having a mass concentration of 0.5%, and then sprayed to petroleum-contaminated soil.
In a further embodiment, the rhamnolipid is added in an amount of 60-120mg per 1kg of petroleum contaminated soil. In the course of applying the surfactant solution, when the added amount of the surfactant solution is in the range of 60mg/1kg of soil to 120mg/1kg of soil, the petroleum pollutants in the soil can be effectively eluted.
In some embodiments, prior to step S1, the treatment method of the present invention further comprises crushing the petroleum-contaminated soil to have a particle size of 10mm or less. Before adding the microbial agent and/or the soil conditioner into the petroleum-polluted soil, firstly, the petroleum-polluted soil can be excavated and crushed into soil particles with the particle size of less than or equal to 10mm by using a soil crushing device. Soil crushing devices that may be used in the present application include, but are not limited to, soil crushers, single shaft crushers, twin shaft crushers, and stone crushers. After the crushing, the soil particles with the diameter less than or equal to 10mm can be uniformly mixed with the microbial agent and/or the soil improvement agent, so that the excellent degradation effect of the petroleum pollutants is realized. In addition, because the broken soil is looser, the water retention of the broken soil is improved, the growth of the plants planted in the step S2 is facilitated, and the speed of restoring the soil by the plants is accelerated.
In a further embodiment, between step S1 and step S2, the processing method further comprises stacking the petroleum-contaminated soil at a thickness of 50cm to 70 cm. After adding the microbial agent and/or the soil conditioner to the petroleum-contaminated soil, the method of the present application further comprises backfilling the uniformly mixed soil into the excavation area (petroleum-contaminated area) at a thickness of 50cm to 70 cm. An excessively thick soil layer thickness (for example, more than 70cm) is disadvantageous for oxygen permeation, since microorganisms mixed in the soil need to grow in an aerobic environment. When the thickness is less than 50cm, the humidity in the soil is not kept favorably, the soil is stacked too thin, and the repairing amount is small each time.
In a further embodiment, the moisture content of the petroleum-contaminated soil is in the range of 15% to 20%. Since the present invention employs a combination of a surfactant, microorganisms and plants, the humidity should be controlled in the range of 15% to 20%. Humidity below 15% and humidity above 20% are not conducive to the growth and survival of plants and microorganisms. In particular embodiments, periodic watering, drip irrigation, application of mulching, etc. may be used to maintain soil moisture in the range of 15% to 20%.
In some embodiments of the present application, the treatment process of the present application is carried out for 30 days to 6 months to achieve removal of petroleum contaminants, and preferably the treatment process is carried out at a temperature in the range of 10 ℃ to 40 ℃. Since the growth of the microorganisms needs to be carried out at a suitable temperature, it is preferable in the present application to carry out the treatment process at a temperature of 10 to 40 c, thereby achieving the optimal petroleum pollutant removal effect.
In a further embodiment, the processing method of the present application further comprises repeating step S3 every 30 to 40 days. Since the surfactant used in step S3 is environmentally friendly, it will be completely degraded in natural environment within 1 to 2 months. To further effect the treatment method of the present application, the surfactant solution is repeatedly applied every 30 to 40 days so that the concentration of the surfactant can be maintained at a high level in the soil to be treated, thereby accelerating the degradation rate of the petroleum pollutants.
The present application is described in further detail below with reference to specific examples, which should not be construed as limiting the scope of the invention as claimed.
Example 1
4000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is smaller than 10 mm. Detecting the content of the petroleum pollutants in the soil to be 59425mg/kg, and taking 1000kg of petroleum polluted soil to carry out a restoration test.
Uniformly mixing 457ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 800mL of the complex microbial inoculum is mixed with 39.2kg of the carrier to prepare the microbial agent (the adding ratio of the complex microbial inoculum to the carrier is 20mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1:1.
The soil conditioner was prepared by uniformly mixing 8.3kg of urea, 0.8kg of diammonium phosphate, and 0.9kg of potassium humate.
Uniformly adding 40kg of microbial agent and 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 70 cm.
The reed seedlings are 50 plants/m2The planting density of (2) was planted on the soil to be treated (total 1 square meter, 50 plants were planted).
480g of rhamnolipid purified product with the mass concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution every 30 days. (addition ratio 120mg/kg soil)
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 15%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 2816 mg/kg.
See figure 1 and table 1 for results.
Comparative example 1
1000kg of soil with the petroleum pollutant content of 59425mg/kg in example 1 was taken for remediation.
Uniformly mixing 457ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 800mL of the complex microbial inoculum is mixed with 39.2kg of the carrier to prepare the microbial agent (the adding ratio of the complex microbial inoculum to the carrier is 20mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1:1.
The soil conditioner was prepared by uniformly mixing 8.3kg of urea, 0.8kg of diammonium phosphate, and 0.9kg of potassium humate.
Uniformly adding 40kg of microbial agent and 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 70 cm.
The reed seedlings are 50 plants/m2The planting density of (2) was planted on the soil to be treated (total 1 square meter, 50 plants were planted).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 15%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 29147 mg/kg.
See figure 1 and table 1 for results.
Comparative example 2
1000kg of soil with the petroleum pollutant content of 59425mg/kg in example 1 was taken for remediation.
The soil conditioner was prepared by uniformly mixing 8.3kg of urea, 0.8kg of diammonium phosphate, and 0.9kg of potassium humate.
Uniformly adding 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 70 cm.
The reed seedlings are 50 plants/m2The planting density of (2) was planted on the soil to be treated (total 1 square meter, 50 plants were planted).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 15%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 38199 mg/kg.
See figure 1 and table 1 for results.
Comparative example 3
1000kg of soil with the petroleum pollutant content of 59425mg/kg in example 1 was taken for remediation.
Uniformly mixing 457ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 800mL of the complex microbial inoculum is mixed with 39.2kg of the carrier to prepare the microbial agent (the adding ratio of the complex microbial inoculum to the carrier is 20mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1:1.
The soil conditioner was prepared by uniformly mixing 8.3kg of urea, 0.8kg of diammonium phosphate, and 0.9kg of potassium humate.
Uniformly adding 40kg of microbial agent and 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 70 cm.
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 15%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 42522 mg/kg.
See figure 1 and table 1 for results.
Example 2
4000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is smaller than 10 mm. And (3) detecting that the content of the petroleum pollutants in the soil is 48059mg/kg, and taking 1000kg of petroleum-polluted soil to perform a remediation test.
Evenly mixing 343ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 443mL of the complex microorganism solution is mixed with 29.57g of the carrier to prepare the microorganism agent (the adding ratio of the complex microorganism solution to the carrier is 15mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.5.
6.48kg of urea, 0.72kg of diammonium hydrogen phosphate and 0.8kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 30kg of microbial agent and 8kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 60 cm.
The reed seedlings are planted at a rate of 40 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.2 square meters, 48 plants were planted).
360g of rhamnolipid purification product with the mass concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution (added at a ratio of 90mg/kg soil) once every 35 days.
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 17 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 3549 mg/kg.
See figure 2 and table 1 for results.
Comparative example 4
1000kg of the soil with the petroleum pollutant content of 48059mg/kg in example 2 was taken for remediation.
Evenly mixing 343ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 443mL of the complex microorganism solution is mixed with 29.57g of the carrier to prepare the microorganism agent (the adding ratio of the complex microorganism solution to the carrier is 15mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.5.
6.48kg of urea, 0.72kg of diammonium hydrogen phosphate and 0.8kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 30kg of microbial agent and 8kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 60 cm.
The reed seedlings are planted at a rate of 40 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.2 square meters, 48 plants were planted).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 17 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 26452 mg/kg.
See figure 2 and table 1 for results.
Comparative example 5
1000kg of the soil with the petroleum pollutant content of 48059mg/kg in example 2 was taken for remediation.
6.48kg of urea, 0.72kg of diammonium hydrogen phosphate and 0.8kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 8kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 60 cm.
Reed is made of reedSeedlings were grown at 40 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.2 square meters, 48 plants were planted).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 17 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 31987 mg/kg.
See figure 2 and table 1 for results.
Comparative example 6
1000kg of the soil with the petroleum pollutant content of 48059mg/kg in example 2 was taken for remediation.
Evenly mixing 343ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 443mL of the complex microorganism solution is mixed with 29.57g of the carrier to prepare the microorganism agent (the adding ratio of the complex microorganism solution to the carrier is 15mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.5.
6.48kg of urea, 0.72kg of diammonium hydrogen phosphate and 0.8kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 30kg of microbial agent and 8kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 60 cm.
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 17 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 32950 mg/kg.
See figure 2 and table 1 for results.
Example 3
4000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is smaller than 10 mm. Detecting the content of the petroleum pollutants in the soil to be 39572mg/kg, and taking 1000kg of petroleum polluted soil to carry out a restoration test.
Evenly mixing 343ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 200mL of the complex microbial inoculum is mixed with 19.8kg of the carrier to prepare the microbial agent (the adding ratio of the complex microbial inoculum to the carrier is 10mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.2.
3.9kg of urea, 0.5kg of diammonium hydrogen phosphate and 0.6kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 20kg of microbial agent and 5kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 50 cm.
The reed seedlings are planted at the rate of 30 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.4 square meters, 42 plants were planted).
240g of rhamnolipid purification product with the mass concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution every 40 days. (addition ratio 60mg/kg soil).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 20%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 2392 mg/kg.
See figure 3 and table 1 for results.
Comparative example 7
1000kg of soil with the petroleum pollutant content of 39572mg/kg in example 3 was taken for remediation.
Evenly mixing 343ml of pseudomonas aeruginosa bacterial liquid, 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 200mL of the complex microbial inoculum is mixed with 19.8kg of the carrier to prepare the microbial agent (the adding ratio of the complex microbial inoculum to the carrier is 10mL/kg of the carrier). CarrierThe body is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.2.
3.9kg of urea, 0.5kg of diammonium hydrogen phosphate and 0.6kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 20kg of microbial agent and 5kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 50 cm.
The reed seedlings are planted at the rate of 30 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.4 square meters, 42 plants were planted).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 20%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 9760 mg/kg.
See figure 3 and table 1 for results.
Comparative example 8
1000kg of soil with the petroleum pollutant content of 39572mg/kg in example 3 was taken for remediation.
3.9kg of urea, 0.5kg of diammonium hydrogen phosphate and 0.6kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 5kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 50 cm.
The reed seedlings are planted at the rate of 30 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.4 square meters, 42 plants were planted).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 20%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 20841 mg/kg.
See figure 3 and table 1 for results.
Comparative example 9
1000kg of soil with the petroleum pollutant content of 39572mg/kg in example 3 was taken for remediation.
343ml of pseudomonas aeruginosaThe bacterial liquid of (1), 229ml of bacillus subtilis bacterial liquid and 114ml of aspergillus versicolor bacterial liquid are uniformly mixed to prepare the composite microbial liquid, wherein the concentration of the bacterial strain in each bacterial liquid is 109cfu/ml. 200mL of the complex microbial inoculum is mixed with 19.8kg of the carrier to prepare the microbial agent (the adding ratio of the complex microbial inoculum to the carrier is 10mL/kg of the carrier). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.2.
3.9kg of urea, 0.5kg of diammonium hydrogen phosphate and 0.6kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 20kg of microbial agent and 5kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 50 cm.
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 20%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 24974 mg/kg.
See figure 3 and table 1 for results.
Example 4
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 45278 mg/kg.
The strain concentration is 6X 109cfu/mL of 200mL of pseudomonas aeruginosa bacterial solution and 19.8kg of cottonseed hull carrier are mixed to prepare the microbial agent (the adding ratio of the compound microbial solution to the carrier is 10mL/kg of carrier).
The soil conditioner is prepared by uniformly mixing 3.9kg of urea, 0.5kg of diammonium hydrogen phosphate and 0.6kg of potassium humate.
Uniformly adding 20kg of microbial agent and 5kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 70 cm.
The reed seedlings are planted at a rate of 45 plants/m2The planting density of (2) is planted on the soil to be treated (total 1 square meter, 45 plants are planted)。
240g of rhamnolipid purification product with the mass concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution every 40 days. (addition ratio 60mg/kg soil).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 20%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 30 days of remediation, and the oil content after treatment is 39608 mg/kg.
See table 1 for results.
Example 5
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 47616 mg/kg.
The strain concentration is 5X 109cfu/mL 780mL bacillus subtilis liquid and 39.2g rice hull carrier are mixed to prepare microbial agent (the adding ratio of the compound microbial liquid to the carrier is 20mL/kg)
The soil conditioner was prepared by uniformly mixing 8kg of urea, 0.8kg of diammonium phosphate, and 1.2kg of potassium humate.
Uniformly adding 40kg of microbial agent and 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 60 cm.
The reed seedlings are 35 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.2 square meters, 42 plants were planted).
320g of rhamnolipid purified product with the concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution (added at a ratio of 80mg/kg soil) once every 30 days.
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 18 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil 120 days after the petroleum polluted soil is repaired, and the oil content after treatment is 23525 mg/kg.
See table 1 for results.
Example 6
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 43899 mg/kg.
The strain concentration is 6X 109780mL of the bacterial solution of the aspergillus versicolor of cfu/mL is mixed with 39.2kg of bran carriers to prepare the microbial agent (the adding ratio of the compound microbial bacterial solution to the carriers is 20 mL/kg).
A soil conditioner was prepared by mixing 7.9kg of urea, 1.2kg of diammonium phosphate, and 0.9kg of potassium humate.
Uniformly adding 40kg of microbial agent and 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 65 cm.
The reed seedlings are planted at the rate of 32 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.1 square meter, 35 plants were planted).
320g of rhamnolipid purification product with the mass concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution every 30 days. (addition ratio 80mg/kg soil).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 15%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 19359 mg/kg.
See table 1 for results.
Example 7
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 44628 mg/kg.
The 265ml of pseudomonas aeruginosa bacterial liquid and the 265ml of bacillus subtilis bacterial liquid are evenly mixed to prepareThe concentration of the strain in each bacterial liquid is 3 multiplied by 109cfu/ml. 530mL of the complex microbial inoculum was mixed with 29.47kg of an activated carbon carrier to prepare a microbial inoculum (18 mL/kg of the complex microbial inoculum added to the carrier).
7.8kg of urea, 1.3kg of diammonium hydrogen phosphate and 0.9kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 30kg of microbial agent and 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 70 cm.
The reed seedlings are planted at a rate of 44 plants/m2The planting density of (2) was planted on the soil to be treated (total 1 square meter, 44 plants were planted).
280g of rhamnolipid purified product with the mass concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution (added at a ratio of 70mg/kg soil) once every 40 days.
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 19 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 90 days of remediation, and the oil content after treatment is 20952 mg/kg.
See table 1 for results.
Example 8
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 45547 mg/kg.
Evenly mixing 335ml of aspergillus versicolor bacterial liquid and 168ml of bacillus subtilis bacterial liquid to prepare composite microbial liquid, wherein the concentration of the bacterial strain in each bacterial liquid is 4 multiplied by 109cfu/ml. 503mL of complex microbial solution was mixed with 31.5kg of a carrier to prepare a microbial agent (the ratio of complex microbial solution to carrier added was 16 mL/kg). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.3.
4.98kg of urea, 0.48kg of diammonium hydrogen phosphate and 0.54kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 32kg of microbial agent and 6kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 63 cm.
The reed seedlings are cultured at 37 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.1 square meter, 42 plants were planted).
292g of rhamnolipid purification product with the mass concentration of 25 percent is prepared into a solution with the mass concentration of 0.5 percent and sprayed on the petroleum polluted soil. And spraying rhamnolipid solution every 40 days. (addition ratio 73mg/kg soil)
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 20%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 75 days of remediation, and the oil content after treatment is 26240 mg/kg.
See table 1 for results.
Example 9
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 42713 mg/kg.
Uniformly mixing 201ml of pseudomonas aeruginosa bacterial liquid and 301ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 2 multiplied by 109cfu/ml. 502mL of the complex microbial solution was mixed with 33.5kg of the carrier to prepare a microbial agent (the ratio of the complex microbial solution to the carrier was 15 mL/kg). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.7.
7.29kg of urea, 0.81kg of diammonium hydrogen phosphate and 0.9kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 34kg of microbial agent and 9kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 52 cm.
Seedling of reedAt 50 strains/m2The planting density of (2) was planted on the soil to be treated (total 1.37 square meters, 68 plants were planted).
420g of rhamnolipid purified product with the mass concentration of 25% is prepared into a solution with the mass concentration of 0.5%, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution every 30 days. (addition ratio 105mg/kg soil)
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 17 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 60 days of remediation, and the oil content after treatment is 29054 mg/kg.
See table 1 for results.
Example 10
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 46521 mg/kg.
Uniformly mixing 126ml of pseudomonas aeruginosa bacterial liquid, 188ml of bacillus subtilis bacterial liquid and 126ml of aspergillus versicolor bacterial liquid in proportion to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 440mL of the complex microbial inoculum was mixed with 29.56kg of the carrier to prepare the microbial inoculum (15 mL/kg of the complex microbial inoculum added to the carrier ratio). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 2.
5.46kg of urea, 0.91kg of diammonium hydrogen phosphate and 0.63kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 30kg of microbial agent and 7kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 50 cm.
The reed seedlings are planted at the rate of 30 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.4 square meters, 42 plants were planted).
480g of 25% sophorolipid purified product is prepared into 0.5% solution by mass concentration and sprayed on the petroleum polluted soil. And spraying rhamnolipid solution every 30 days. (addition ratio 120mg/kg soil).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 18 percent.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 100 days of remediation, and the oil content after treatment is 18668 mg/kg.
See table 1 for results.
Example 11
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of petroleum pollutants in the soil is detected to be 47954 mg/kg.
Uniformly mixing 196ml of pseudomonas aeruginosa bacterial liquid, 146ml of bacillus subtilis bacterial liquid and 98ml of aspergillus versicolor bacterial liquid in proportion to prepare the composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 440mL of the complex microbial inoculum was mixed with 29.56kg of the carrier to prepare the microbial inoculum (15 mL/kg of the complex microbial inoculum added to the carrier ratio). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1: 1.5.
6.4kg of urea, 0.64kg of diammonium phosphate and 0.96kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 30kg of microbial agent and 8kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 65 cm.
The reed seedlings are planted at a rate of 45 plants/m2The planting density of (2) was planted on the soil to be treated (total 1.1 square meter, 49 plants were planted).
380g of lipopeptide purified product with the mass concentration of 25% is prepared into 0.5% solution with the mass concentration, and the solution is sprayed on the petroleum-polluted soil. And spraying rhamnolipid solution every 30 days. (addition ratio 95mg/kg soil).
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 20%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil after 180 days of remediation, and the oil content after treatment is 4615 mg/kg.
See table 1 for results.
Example 12
1000kg of petroleum-polluted soil is mined and dug from a petroleum-polluted area to be treated, and a soil crusher is used for crushing, so that the particle size of crushed soil particles is less than 10 mm. The content of the petroleum pollutants in the soil is detected to be 45878 mg/kg.
Uniformly mixing 138ml of pseudomonas aeruginosa bacterial liquid, 138ml of bacillus subtilis bacterial liquid and 70ml of aspergillus versicolor bacterial liquid to prepare composite microbial bacterial liquid, wherein the concentration of bacterial strains in each bacterial liquid is 109cfu/ml. 346mL of complex microbial solution was mixed with 34.65kg of carrier to prepare a microbial preparation (the ratio of complex microbial solution to carrier was 10 mL/kg). The carrier is prepared from cottonseed hulls and rice hulls according to the mass ratio of 1:1.
7.9kg of urea, 1.2kg of diammonium hydrogen phosphate and 0.9kg of potassium humate are uniformly mixed to prepare the soil conditioner.
Uniformly adding 35kg of microbial agent and 10kg of soil conditioner into the petroleum-polluted soil; and after uniformly mixing, stacking the mixture in a degradation field for anti-seepage treatment, wherein the stacking thickness is 70 cm.
The reed seedlings are planted at a rate of 40 plants/m2The planting density of (2) was planted on the soil to be treated (total 1 square meter, 40 plants were planted).
300g of purified natural soap powder with the mass concentration of 25 percent is prepared into a solution with the mass concentration of 0.5 percent and is sprayed on the petroleum polluted soil. And spraying rhamnolipid solution (added at a ratio of 75mg/kg soil) once every 30 days.
Periodically spraying water to the petroleum-polluted soil to keep the water content of the petroleum-polluted soil at 15%.
Under outdoor conditions, oil content detection is carried out on the petroleum polluted soil 120 days after the petroleum polluted soil is repaired, and the oil content after treatment is 14522 mg/kg.
See table 1 for results.
The results of the experiment are shown in table 1 below.
TABLE 1
| Oil content of soil before treatment | Oil content of treated soil | Percent removal | Time of treatment | |
| Example 1 | 59425mg/kg | 2816mg/kg | 95.26％ | 180 days |
| Comparative example 1 | 59425mg/kg | 29147mg/kg | 50.95％ | 180 days |
| Comparative example 2 | 59425mg/kg | 38199mg/kg | 35.72％ | 180 days |
| Comparative example 3 | 59425mg/kg | 42522mg/kg | 28.44％ | 180 days |
| Example 2 | 48059mg/kg | 3549mg/kg | 92.62％ | 180 days |
| Comparative example 4 | 48059mg/kg | 26452mg/kg | 44.96％ | 180 days |
| Comparative example 5 | 48059mg/kg | 31987mg/kg | 33.44％ | 180 days |
| Comparative example 6 | 48059mg/kg | 32950mg/kg | 31.44％ | 180 days |
| Example 3 | 39572mg/kg | 2392mg/kg | 93.96％ | 180 days |
| Comparative example 7 | 39572mg/kg | 16760mg/kg | 57.65％ | 180 days |
| Comparative example 8 | 39572mg/kg | 20841mg/kg | 47.33％ | 180 days |
| Comparative example 9 | 39572mg/kg | 24974mg/kg | 36.89％ | 180 days |
| Example 4 | 45278mg/kg | 38608mg/kg | 12.52％ | 30 days |
| Example 5 | 47616mg/kg | 23525mg/kg | 50.59％ | 120 days |
| Example 6 | 43899mg/kg | 19359mg/kg | 55.9％ | 150 days |
| Example 7 | 44628mg/kg | 20952mg/kg | 46.33％ | 90 days |
| Example 8 | 45547mg/kg | 26240mg/kg | 38％ | 75 days |
| Example 9 | 42713mg/kg | 29054mg/kg | 28％ | 60 days |
| Example 10 | 46521mg/kg | 18668mg/kg | 59.87％ | 100 days |
| Example 11 | 47954mg/kg | 4615mg/kg | 90.38％ | 150 days |
| Example 12 | 45878mg/kg | 14522mg/kg | 68.35％ | 120 days |
It can be seen from the above experimental results that the method of the present application can effectively solve the problems of long repair cycle, poor efficiency, etc. of the method in the prior art. It can be seen from a comparison of examples 1-3 with comparative examples 1-9 and from fig. 1-3 that the removal rate of petroleum pollutants in soil can reach 95% or more using the method of the present application, whereas the prior art method comprising only plant growing and/or microbial agents can achieve removal rates of 50% or less at the same time. As can be seen from fig. 1-3, the concentration of petroleum pollutants in the soil rapidly decreased with the days of remediation and decreased to less than 5% of the initial concentration at 180 days using the method of the present application, thereby achieving rapid remediation of the soil. Furthermore, it can be seen from examples 4 to 12 that, in the case of using the method of the present application, the removal effect can be started to be exerted in a short period of time (examples 4 and 9) and the removal effect that could be achieved in the prior art for 180 days can be achieved in the case of only 90 days (example 7). Therefore, the repair time of the soil polluted by the stones is obviously shortened and the repair efficiency is improved under the condition of using the method.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (14)
1. A method for treating petroleum-contaminated soil, comprising:
step S1, adding a microbial agent into the petroleum-polluted soil;
step S2, planting plants on the petroleum-polluted soil;
and step S3, spraying a surfactant solution to the petroleum-polluted soil.
2. The process of claim 1, wherein the microbial agent comprises a microbial fluid and a carrier.
3. The process according to claim 2, wherein the microbial liquid comprises a combination of any one or more of Pseudomonas aeruginosa (Pseudomonas aeruginosa), Bacillus subtilis (Bacillus subtilis), Aspergillus versicolor (Aspergillus versicolor); preferably, the microbial liquid comprises a liquid of pseudomonas aeruginosa, a liquid of bacillus subtilis and a liquid of aspergillus versicolor; more preferably, the volume ratio of the pseudomonas aeruginosa bacterial liquid to the bacillus subtilis bacterial liquid to the aspergillus versicolor bacterial liquid is 1-2: 1-1.5: 0.5-1, and the strain concentrations in the pseudomonas aeruginosa bacterial liquid, the bacillus subtilis bacterial liquid and the aspergillus versicolor bacterial liquid are respectively and independently more than or equal to 1 × 109cfu/ml。
4. The method according to claim 2, wherein the amount of the microbial cell broth is 10 to 20mL per 1kg of the vector, and preferably the strain concentrations in the Pseudomonas aeruginosa broth, the Bacillus subtilis broth, and the Aspergillus versicolor broth are each independently 1X 109cfu/ml to 6X 109In the cfu/ml range.
5. The treatment method of claim 2, wherein the carrier comprises any one or a combination of more of cottonseed hulls, rice hulls, bran, corn cobs, and activated carbon; preferably, said carrier comprises said cottonseed hulls and said rice hulls; further preferably, the weight ratio of the cottonseed hulls to the rice hulls is in the range of 1: 1-2.
6. The process of claim 1, wherein the amount of the microbial agent is 2 to 4 wt% based on the total weight of the petroleum-contaminated soil.
7. The process of claim 1, wherein the step S1 further comprises adding a soil amendment to the petroleum-contaminated soil, preferably in an amount of 0.5 to 1 wt% based on the total weight of the petroleum-contaminated soil, preferably in a soil amendment in which the ratio of nitrogen: phosphorus: the weight ratio of potassium is 17-22: 0.8-1.2: 1.3-1.6; preferably, the soil amendment comprises a combination of any of urea, ammonium bicarbonate, diammonium phosphate, calcium superphosphate, dipotassium phosphate trihydrate, potassium chloride, and potassium humate; further preferably, the soil conditioner comprises 78-83 wt% of urea, 8-13 wt% of diammonium hydrogen phosphate and 9-14 wt% of potassium humate.
8. The treatment method according to claim 1, wherein the plant comprises any one or more of alfalfa, cotton, haloxylon and reed in combination, preferably the plant is reed.
9. The treatment method according to claim 1, wherein the plants are planted at a density of 30 plants/m2To 50 strains/m2。
10. The treatment method as claimed in claim 1, wherein the surfactant solution comprises rhamnolipid, sophorolipid, lipopeptide, natural soap powder, etc.; preferably, the surfactant is a rhamnolipid; further preferably, the addition amount of the rhamnolipid per kilogram of petroleum-polluted soil is 60-120 mg; further preferably, the rhamnolipid is prepared into a solution with the mass concentration of 0.5 percent and then sprayed to the petroleum-polluted soil.
11. The treatment method as claimed in claim 1, wherein before the step S1, the treatment method further comprises crushing the petroleum-contaminated soil so that the particle size of the petroleum-contaminated soil is 10mm or less.
12. The process according to claim 1 or 7, wherein between the step S1 and the step S2, the process further comprises stacking the oil contaminated soil at a thickness of 50cm to 70cm, preferably on an impermeable treated site.
13. The process of claim 1, wherein the moisture content of the petroleum-contaminated soil is in the range of 15% to 20%.
14. The treatment process according to claim 1, wherein the treatment process is carried out at a temperature in the range of 10 ℃ to 40 ℃, preferably the treatment process further comprises repeating the step S3 every 30 days to 40 days.
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