CN114088501A - Chip device for in-situ tissue staining and decoloring and use method - Google Patents
Chip device for in-situ tissue staining and decoloring and use method Download PDFInfo
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- CN114088501A CN114088501A CN202111336698.0A CN202111336698A CN114088501A CN 114088501 A CN114088501 A CN 114088501A CN 202111336698 A CN202111336698 A CN 202111336698A CN 114088501 A CN114088501 A CN 114088501A
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- 238000010186 staining Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 95
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 239000003792 electrolyte Substances 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 9
- 238000004043 dyeing Methods 0.000 claims description 9
- 229920000089 Cyclic olefin copolymer Polymers 0.000 claims description 8
- 239000012192 staining solution Substances 0.000 claims description 6
- 239000004713 Cyclic olefin copolymer Substances 0.000 claims description 4
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 4
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 3
- 230000001464 adherent effect Effects 0.000 claims description 3
- 238000000799 fluorescence microscopy Methods 0.000 claims description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- 239000010931 gold Substances 0.000 claims description 3
- 229910052737 gold Inorganic materials 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 abstract description 5
- 230000005779 cell damage Effects 0.000 abstract description 2
- 208000037887 cell injury Diseases 0.000 abstract description 2
- 238000003384 imaging method Methods 0.000 abstract description 2
- 239000012535 impurity Substances 0.000 abstract description 2
- 230000000007 visual effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 10
- 206010028980 Neoplasm Diseases 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 3
- 238000009169 immunotherapy Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 230000002055 immunohistochemical effect Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011982 device technology Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
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- Immunology (AREA)
- Pathology (AREA)
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Abstract
The invention discloses a chip device for in-situ tissue staining and decoloring, which comprises three layers of structures which are stacked together and sealed with each other, wherein a top cover, a base plate and a bottom plate are respectively arranged from top to bottom; the top cover is provided with a liquid inlet and a liquid outlet, one side of the bottom surface of the top cover is concavely provided with a groove, and two ends of the groove are communicated with the liquid inlet and the liquid outlet; the substrate is provided with a groove corresponding to the bottom surface of the top cover to serve as a micro-flow channel; the microfluidic channel is divided into a liquid inlet area, a central area, a tissue fixing area and a liquid outlet area from left to right; the bottom plate is provided with positive and negative electrodes, and the electrodes consist of electrode bodies and electrode pins; the electrode body of the positive electrode is exposed in the microfluidic channel and corresponds to the liquid inlet; the electrode body of the negative electrode is located in the central region. The chip device provided by the invention is operated internally, so that impurity pollution is avoided, the accuracy of analysis is improved, and the chip device is decolorized by an electrochemical means, so that the time consumption is short and no cell damage is caused; the method has the visual characteristic and is convenient for imaging analysis.
Description
Technical Field
The invention relates to a chip device for in-situ tissue staining and decoloring and a using method thereof, belonging to the technical field of medical inspection and analysis.
Background
The multicolor Immunohistochemistry (miHC) is a multi-label staining technology which simultaneously or sequentially adopts fluorescein or enzymatic products with different colors on the same slice by utilizing the principles of immunology and cytochemistry to trace different antigen macromolecular substances in tissues or cells in situ.
The multicolor immunohistochemical technology is carried along with the precise immunotherapy of the tumor, and the phenotypes of various cells in the tumor microenvironment and the spatial relationship of the cells can be directly evaluated to reflect the immune state of the tumor microenvironment. The technology can save more time and utilize rare tissue samples to a greater extent, and can help researchers comprehensively, deeply and accurately know the conditions of local tissues and microenvironment of the tumor. A set of personalized immunotherapy scheme aiming at local tumor is formulated according to the tumor antigen expression and growth characteristics in a tumor microenvironment, the number, phenotype and functional state of infiltrating immune cells, the concentration and type of cytokines and other information, so that the goal is achieved. Because the technology can carry out high-efficiency, fine and accurate determination, the technology becomes an important means of accurate immunotherapy of tumors, and has huge clinical application prospect.
The key points of the multicolor immunohistochemical technique include: tissue staining and tissue destaining. The tissue staining is a process in which a substance having a fluorescent signal and a substance capable of binding to a protein on the surface of a tissue section are bound and labeled on the surface of a tissue cell, so that the labeled site can be confirmed based on the fluorescent signal. Tissue destaining, i.e., the removal of the signal of a labeled staining substance on the surface of a tissue cell using a technique.
The existing tissue decolorization technologies, including photobleaching, acid-base solution elution and oxidation, have the defects of low efficiency, long time consumption, cell surface damage and the like in the tissue decolorization process.
Disclosure of Invention
Aiming at the problems, the invention provides a chip device for in-situ tissue staining and decoloring and a using method thereof. The device technology can conveniently carry out fluorescence staining on the tissue cells and quickly and nondestructively remove staining fluorescence signals on the surfaces of the tissue cells.
The invention adopts the following technical scheme:
a chip device for in-situ tissue staining and decoloring comprises three layers of structures which are stacked together and sealed with each other, wherein the three layers of structures are respectively a top cover, a base plate and a bottom plate from top to bottom;
the top cover is provided with a liquid inlet and a liquid outlet, and the liquid inlet is provided with a liquid inlet pipe; a liquid outlet pipe is arranged on the liquid outlet; a groove is concavely formed on one side of the bottom surface of the top cover, and two ends of the groove are communicated with the liquid inlet and the liquid outlet;
the substrate is provided with a groove corresponding to the bottom surface of the top cover and used as a micro-flow channel;
the microfluidic channel is divided into a liquid inlet area, a central area, a tissue fixing area and a liquid outlet area from left to right; the liquid inlet area and the liquid outlet area correspond to the liquid inlet and the liquid outlet respectively, the central area is positioned in the middle of the microfluidic channel, and the tissue fixing area is positioned between the central area and the liquid outlet area;
the bottom plate is provided with positive and negative electrodes, and the electrodes consist of electrode bodies and electrode pins; the electrode body of the positive electrode is positioned in the liquid inlet area and corresponds to the liquid inlet; the electrode body of the negative electrode is positioned in the central area; the electrode pins are exposed in the air and used for being externally connected with a direct current power supply.
Further, the top cover, the substrate and the bottom plate are made of polymethyl methacrylate (PMMA), Polycarbonate (PC), Cyclic Olefin Copolymer (COC) or Cyclic Olefin Polymer (COP).
Further, the thickness of the top cover is 0.5-2.5 mm.
Furthermore, the thickness of the substrate is 0.5-2 mm, and the thickness of the bottom plate is 0.5-1.5 mm.
Further, the tissue fixation region has a thickness of 0.1 mm.
Furthermore, the positive electrode and the negative electrode are hollow gold electrodes, and the thickness of the electrodes is uniform and is 1-100 micrometers.
The use method of the chip device comprises the following steps:
(1) firstly, fixing a tissue slice in a tissue fixing area of a chip;
(2) the liquid inlet pipe and the liquid outlet pipe are respectively connected with liquid inlet and outlet equipment;
(3) injecting tissue staining solution into the liquid inlet through the liquid inlet pipe, stopping injecting after the whole microflow channel is filled with the tissue staining solution, and staining the fixed tissue slice;
(4) after dyeing is finished, injecting cleaning fluid into the liquid inlet, and stopping injecting the dyeing fluid after the cleaning fluid washes out the dyeing fluid from the liquid outlet;
(5) performing fluorescence imaging on the tissue slices;
(6) injecting electrolyte into the liquid inlet, and stopping injecting after the cleaning liquid is flushed out of the liquid outlet, so as to ensure that the whole microflow channel is filled with the electrolyte;
(7) connecting the positive electrode and the negative electrode to an external power output device, electrifying and keeping the voltage at 1.2-10V;
(8) and the power supply is turned off, the electrolyte is continuously injected into the liquid inlet, and the fresh electrolyte pushes the electrolyzed electrolyte to flow through the tissue section area to decolor the tissue.
(9) Finishing the process of single tissue staining and decoloring, and repeating the processes of the steps (1) to (8) for a plurality of times according to requirements.
Further, the tissue slice in the step (1) is animal, human tissue patch or adherent cell.
Advantageous effects
(1) The chip is operated inside, so that impurity pollution is avoided, and the accuracy of analysis is improved.
(2) The electrochemical method is used for decoloring, the time consumption is short, and no cell damage is caused.
(3) Can be integrated and automated, and has clinical and commercial popularization values.
(4) The chip device has small volume and is convenient to store and carry. The chip has the visual characteristic and is convenient for imaging analysis.
(5) Simple operation and no need of complex professional background.
Drawings
Fig. 1 is a schematic diagram of a chip structure according to the present invention.
FIG. 2 is a diagram of a chip according to the present invention. Wherein (1) is a perspective view, (2) is a bottom view, and (3) is a front view. A is liquid inlet area, B is central area, C is tissue fixing area, D is liquid outlet area, E is liquid inlet pipe, F is liquid outlet pipe, G is electrode.
FIG. 3 shows the staining and destaining of tissue sections in-chip.
Fig. 4 is a metal electrode diagram (the positive and negative electrodes have the same structure). Wherein 1-the electrode body; 2-electrode pin.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
The invention adopts the following technical scheme for solving the technical problems:
a chip device for in-situ tissue staining and decolorizing is shown in fig. 1 and 2, and comprises a three-layer structure which is stacked together and sealed with each other, and a top cover, a base plate and a bottom plate are respectively arranged from top to bottom; the top cover, the substrate and the bottom plate are made of plastic materials with good optical permeability and no biotoxicity, and the plastic materials comprise polymethyl methacrylate, polycarbonate, cyclic olefin copolymer or cyclic olefin polymer. The thickness of the top cover is 0.5-2.5 mm. The thickness of the substrate is 0.5-2 mm, and the thickness of the bottom plate is 0.5-1.5 mm. The thickness of the tissue fixation area is 0.1 mm.
The top cover is provided with a liquid inlet and a liquid outlet, and the liquid inlet is provided with a liquid inlet pipe E; a liquid outlet pipe F is arranged on the liquid outlet; a groove is concavely formed on one side of the bottom surface of the top cover, and two ends of the groove are communicated with the liquid inlet and the liquid outlet; the substrate is provided with a groove corresponding to the bottom surface of the top cover and used as a micro-flow channel; the microfluidic channel is divided into a liquid inlet area A, a central area B, a tissue fixing area C and a liquid outlet area D from left to right; the liquid inlet area A and the liquid outlet area D correspond to the liquid inlet and the liquid outlet respectively, the central area B is positioned in the middle of the microfluidic channel, and the tissue fixing area C is positioned between the central area B and the liquid outlet area D;
as shown in fig. 4, the bottom plate is provided with a positive electrode G and a negative electrode G, and the electrodes G are composed of an electrode body 1 and electrode pins 2; the electrode body 1 of the positive electrode is exposed in the microfluidic channel, and the electrode body 1 of the positive electrode is positioned in the liquid inlet area A and corresponds to the liquid inlet; the electrode body 1 of the negative electrode is located in the central region B; the electrode pins 2 are exposed in the air and used for being externally connected with a direct current power supply. The positive and negative electrodes G are hollow gold electrodes with uniform thickness of 1-100 microns.
The use method of the chip device, as shown in fig. 3, includes the following steps:
(1) firstly, fixing a tissue slice in a tissue fixing area C of a chip; the tissue slice is an animal tissue patch, a human tissue patch or adherent cells.
(2) The liquid inlet pipe E and the liquid outlet pipe F are respectively connected with liquid inlet and outlet equipment;
(3) injecting tissue staining solution into the liquid inlet through the liquid inlet pipe E, stopping injecting after the whole microfluidic channel is filled with the tissue staining solution, and staining the fixed tissue slice;
(4) after dyeing is finished, injecting cleaning fluid into the liquid inlet, and stopping injecting the dyeing fluid after the cleaning fluid washes out the dyeing fluid from the liquid outlet;
(5) performing fluorescence imaging on the tissue slices;
(6) injecting electrolyte into the liquid inlet, and stopping injecting after the cleaning liquid is flushed out of the liquid outlet, so as to ensure that the whole microflow channel is filled with the electrolyte;
(7) connecting the positive electrode and the negative electrode to an external power output device, electrifying and keeping the voltage at 1.2-10V;
(8) and (4) closing the power supply, continuously injecting the electrolyte into the liquid inlet, and pushing the electrolyzed electrolyte to flow through the tissue section area C by using the fresh electrolyte to decolorize the tissue.
(9) Finishing the process of single tissue staining and decoloring, and repeating the processes of the steps (1) to (8) for a plurality of times according to requirements.
Claims (8)
1. A chip device for in-situ tissue staining and decoloring is characterized by comprising three layers of structures which are stacked together and sealed with each other, wherein the three layers of structures are respectively a top cover, a base plate and a bottom plate from top to bottom;
the top cover is provided with a liquid inlet and a liquid outlet, and the liquid inlet is provided with a liquid inlet pipe (E); and the liquid outlet is provided with a liquid outlet pipe (F); a groove is concavely formed on one side of the bottom surface of the top cover, and two ends of the groove are communicated with the liquid inlet and the liquid outlet;
the substrate is provided with a groove corresponding to the bottom surface of the top cover and used as a micro-flow channel;
the microfluidic channel is divided into a liquid inlet area (A), a central area (B), a tissue fixing area (C) and a liquid outlet area (D) from left to right; the liquid inlet area (A) and the liquid outlet area (D) correspond to the liquid inlet and the liquid outlet respectively, the central area (B) is positioned in the middle of the microfluidic channel, and the tissue fixing area (C) is positioned between the central area (B) and the liquid outlet area (D);
the bottom plate is provided with a positive electrode (G) and a negative electrode (G), and the electrodes (G) consist of electrode bodies (1) and electrode pins (2); the electrode body (1) of the positive electrode is exposed in the microfluidic channel, and the electrode body (1) of the positive electrode is positioned in the liquid inlet area (A) and corresponds to the liquid inlet; the electrode body (1) of the negative electrode is positioned in the central area (B); the electrode pins (2) are exposed in the air and used for being externally connected with a direct current power supply.
2. The chip device for in situ tissue staining and destaining of claim 1, wherein the thickness of the top cover is 0.5-2.5 mm.
3. The chip device for in-situ tissue staining and decoloring of claim 1, wherein the substrate has a thickness of 0.5 to 2 mm and the bottom plate has a thickness of 0.5 to 1.5 mm.
4. The chip device for in situ tissue staining and destaining of claim 1, wherein the thickness of the tissue fixing region (C) is 0.1 mm.
5. The chip device for in situ tissue staining and destaining of claim 1, wherein the material of the top cover, the substrate and the bottom plate comprises polymethyl methacrylate, polycarbonate, cyclic olefin copolymer or cyclic olefin polymer.
6. The chip device for in-situ tissue staining and decoloring according to claim 1, wherein the positive and negative electrodes (G) are hollow gold electrodes with uniform thickness of 1-100 microns.
7. Use of a chip arrangement according to any of claims 1-6, characterized in that it comprises the following steps:
(1) firstly, fixing a tissue slice in a tissue fixing area (C) of a chip;
(2) the liquid inlet pipe (E) and the liquid outlet pipe (F) are respectively connected with liquid inlet and outlet equipment;
(3) injecting tissue staining solution into the liquid inlet through the liquid inlet pipe (E), stopping injecting after the whole microflow channel is filled with the tissue staining solution, and staining the fixed tissue section;
(4) after dyeing is finished, injecting cleaning fluid into the liquid inlet, and stopping injecting the dyeing fluid after the cleaning fluid washes out the dyeing fluid from the liquid outlet;
(5) performing fluorescence imaging on the tissue slices;
(6) injecting electrolyte into the liquid inlet, and stopping injecting after the cleaning liquid is flushed out of the liquid outlet, so as to ensure that the whole microflow channel is filled with the electrolyte;
(7) connecting the positive electrode and the negative electrode to an external power output device, electrifying and keeping the voltage at 1.2-10V;
(8) turning off the power supply, continuously injecting electrolyte into the liquid inlet, and pushing the electrolyzed electrolyte to flow through the tissue section area (C) by using fresh electrolyte to decolorize the tissue;
(9) finishing the process of single tissue staining and decoloring, and repeating the processes of the steps (1) to (8) for a plurality of times according to requirements.
8. The use of claim 7, wherein the tissue section of step (1) is an animal, a human tissue patch, or adherent cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111336698.0A CN114088501A (en) | 2021-11-12 | 2021-11-12 | Chip device for in-situ tissue staining and decoloring and use method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111336698.0A CN114088501A (en) | 2021-11-12 | 2021-11-12 | Chip device for in-situ tissue staining and decoloring and use method |
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| CN114088501A true CN114088501A (en) | 2022-02-25 |
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| CN202111336698.0A Pending CN114088501A (en) | 2021-11-12 | 2021-11-12 | Chip device for in-situ tissue staining and decoloring and use method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115178311A (en) * | 2022-06-08 | 2022-10-14 | 北京机械设备研究所 | Electrowetting drive chip for controlling various liquid drops and control method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050074890A1 (en) * | 2003-05-19 | 2005-04-07 | Ventana Medical Systems, Inc. | Electrophoretic in situ tissue staining |
| CN1846856A (en) * | 2005-03-30 | 2006-10-18 | 霍夫曼-拉罗奇有限公司 | Device having a self sealing fluid port |
| CN201917487U (en) * | 2010-12-31 | 2011-08-03 | 南京金斯瑞生物科技有限公司 | Instrument used for quick protein dying and transfer printing |
| CN104797920A (en) * | 2012-11-29 | 2015-07-22 | 南京金斯瑞生物科技有限公司 | Automatic staining method and staining device for biopolymer |
| US20150362411A1 (en) * | 2014-06-12 | 2015-12-17 | University Of Notre Dame | Microfluidic devices, systems, and methods for imaging tissue samples |
| CN110296969A (en) * | 2019-07-31 | 2019-10-01 | 昆山迪安医学检验实验室有限公司 | A kind of preparation method of water-soluble biochemical tetrazolium luciferase assay reagent and its detection method of tumor cell activity |
| CN111289335A (en) * | 2020-02-27 | 2020-06-16 | 舒斯云 | HDN staining solution and kit for horseradish peroxidase specific color development |
| CN111413184A (en) * | 2020-05-02 | 2020-07-14 | 上海诒福科技有限公司 | Staining device and biological tissue marking apparatus |
| CN113607796A (en) * | 2021-07-01 | 2021-11-05 | 天津大学 | Microfluid flow/flow rate and component cooperative detection device and application thereof |
-
2021
- 2021-11-12 CN CN202111336698.0A patent/CN114088501A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050074890A1 (en) * | 2003-05-19 | 2005-04-07 | Ventana Medical Systems, Inc. | Electrophoretic in situ tissue staining |
| CN1846856A (en) * | 2005-03-30 | 2006-10-18 | 霍夫曼-拉罗奇有限公司 | Device having a self sealing fluid port |
| CN201917487U (en) * | 2010-12-31 | 2011-08-03 | 南京金斯瑞生物科技有限公司 | Instrument used for quick protein dying and transfer printing |
| CN104797920A (en) * | 2012-11-29 | 2015-07-22 | 南京金斯瑞生物科技有限公司 | Automatic staining method and staining device for biopolymer |
| US20150362411A1 (en) * | 2014-06-12 | 2015-12-17 | University Of Notre Dame | Microfluidic devices, systems, and methods for imaging tissue samples |
| CN110296969A (en) * | 2019-07-31 | 2019-10-01 | 昆山迪安医学检验实验室有限公司 | A kind of preparation method of water-soluble biochemical tetrazolium luciferase assay reagent and its detection method of tumor cell activity |
| CN111289335A (en) * | 2020-02-27 | 2020-06-16 | 舒斯云 | HDN staining solution and kit for horseradish peroxidase specific color development |
| CN111413184A (en) * | 2020-05-02 | 2020-07-14 | 上海诒福科技有限公司 | Staining device and biological tissue marking apparatus |
| CN113607796A (en) * | 2021-07-01 | 2021-11-05 | 天津大学 | Microfluid flow/flow rate and component cooperative detection device and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115178311A (en) * | 2022-06-08 | 2022-10-14 | 北京机械设备研究所 | Electrowetting drive chip for controlling various liquid drops and control method thereof |
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