CN114052143A - Compound preparation for maintaining egg laying peak of laying hens in final egg laying period and application method - Google Patents
Compound preparation for maintaining egg laying peak of laying hens in final egg laying period and application method Download PDFInfo
- Publication number
- CN114052143A CN114052143A CN202111456999.7A CN202111456999A CN114052143A CN 114052143 A CN114052143 A CN 114052143A CN 202111456999 A CN202111456999 A CN 202111456999A CN 114052143 A CN114052143 A CN 114052143A
- Authority
- CN
- China
- Prior art keywords
- laying
- melatonin
- egg
- hens
- compound preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 150000001875 compounds Chemical class 0.000 title claims abstract description 41
- 230000017448 oviposition Effects 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 24
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 claims abstract description 78
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 claims abstract description 77
- 229960003987 melatonin Drugs 0.000 claims abstract description 77
- 230000007613 environmental effect Effects 0.000 claims abstract description 13
- 229920002261 Corn starch Polymers 0.000 claims abstract description 10
- 239000008120 corn starch Substances 0.000 claims abstract description 10
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 6
- 238000009472 formulation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 74
- 235000013601 eggs Nutrition 0.000 description 34
- 241001465754 Metazoa Species 0.000 description 9
- 230000003325 follicular Effects 0.000 description 9
- 102000002322 Egg Proteins Human genes 0.000 description 7
- 108010000912 Egg Proteins Proteins 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 210000003278 egg shell Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 210000002503 granulosa cell Anatomy 0.000 description 5
- 210000001161 mammalian embryo Anatomy 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000000287 oocyte Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000003917 TEM image Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000013020 embryo development Effects 0.000 description 3
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 210000002459 blastocyst Anatomy 0.000 description 2
- -1 brain Chemical compound 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 230000035622 drinking Effects 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 230000000955 neuroendocrine Effects 0.000 description 2
- 230000008182 oocyte development Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 241001260012 Bursa Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 description 1
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 description 1
- 102000006771 Gonadotropins Human genes 0.000 description 1
- 108010086677 Gonadotropins Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000008217 follicular development Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000002622 gonadotropin Substances 0.000 description 1
- 229940094892 gonadotropins Drugs 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/184—Hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a compound preparation for maintaining an egg laying peak of a laying hen at the final stage of egg laying and an application method, and belongs to the technical field of laying hen feeding. The compound preparation and the application method are that the melatonin and the corn starch are uniformly added into the feed for feeding, and the feed is used for laying hens at the final stage of egg laying. The method can effectively reduce the degradation of the melatonin, avoid the characteristic of poor water solubility of the melatonin, improve the utilization rate of the melatonin of the laying hens, is simple to operate, and reduces the labor cost. The final concentration of the melatonin is 1mg/kg when the environmental temperature is lower than 35 ℃, and is 2mg/kg when the environmental temperature is higher than 35 ℃.
Description
Technical Field
The invention relates to the technical field of laying hen feeding, in particular to a compound preparation for maintaining an egg laying peak of a laying hen at the final stage of egg laying and an application method.
Background
Melatonin (MT) was originally an indole hormone extracted from the fruit body of bovine pineal, and was named N-acetyl-5-methoxytryptamine. It has been found that many organs other than the pine cone have a function of secreting melatonin, such as brain, spinal cord, gastrointestinal tract, retina, ovary, and testis. Melatonin has molecular weight of 232kDa, is a small molecular substance, has hydrophilicity and lipophilicity, can pass through blood brain barrier and placenta barrier, and plays a role in organs such as animal brain, kidney, liver, ovary and uterus. Melatonin, a neuroendocrine hormone of Chinese medicine, has the effect of regulating various physiological functions of animals, such as sleep, neuroendocrine, immune system and female reproduction. Recent researches show that melatonin plays an important role in the processes of secretion of steroid hormones of animals, follicular development, oocyte maturation, ovulation, embryonic development and the like, and the addition of a proper amount of melatonin can promote the development of follicles and is beneficial to improving the production capacity of animals.
In the field of animal reproduction, melatonin plays a significant role in oocyte development and mature embryo development. In vitro melatonin supplementation can effectively improve oocyte development and maturation capability and improve bursa odds ratio. Enhance the embryo development ability and the embryo quality. In the research of pigs, the external addition of melatonin can obviously improve the quality and the development degree of porcine oocytes, improve the mature follicle, embryo cleavage rate and blastocyst rate of pigs, improve the embryo quality and increase the implantation rate[1,2]. It has been shown that during the IVM process of goats, 1X 10 was added-7The mol/L melatonin can effectively reduce the ROS level of the oocyte, and improve the developmental capacity and the embryo quality of the oocyte[3](ii) a In the study of mouse embryo in vitro transplantation, 1X 10 was implanted-7The blastocyst rate of mol/L melatonin mice is increased, the pregnancy rate is increased from 67.8 percent to 95.0 percent, and the farrowing rate is increased from 2.7 mice per litterThe survival rate of the offspring is remarkably improved from 81.24 percent to 96.84 percent when the survival rate is increased to 4.1 per litter[4]。
In China, which is the first country in the world of laying hen breeding and total egg production, the Chinese laying hen breeding development mode has been changed into high-quality and high-yield breeding through the initial blind expansibility breeding. The '500 eggs produced in 100 weeks' is a new challenge facing the laying hen breeding industry in China. In the research of laying hens, the laying rate of laying hens in the later period of laying can be increased by 7.93 +/-0.91 percent at most, and meanwhile, the concentrated protein height, the eggshell thickness and the eggshell strength of eggs can be improved[5,6]. Related researches on laying hens in the later period of laying have proved that melatonin can improve the secretion of gonadotropins (FSH and LH) and the expression of receptor mRNA through a G protein-coupled receptor pathway mediated by MTR, and improve the yield and the egg quality of the laying hens. However, specific use methods and dosage of melatonin at the final stage of egg laying of laying hens are not clearly researched at present, so that a melatonin compound preparation capable of maintaining the peak of the laying hens at the final stage of egg laying for a long time and an application method thereof are necessary.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a compound preparation for maintaining the egg-laying peak of a laying hen at the final stage of egg laying and an application method thereof, and the compound preparation fed to the laying hen at the final stage of egg laying can effectively prolong and maintain the egg-laying peak of the laying hen for at least 12 weeks, reduce the breakage rate of eggs and ensure that the egg quality of the eggs is not reduced.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
in one aspect, the invention provides a compound preparation for maintaining the egg laying peak of laying hens at the final stage of egg laying, the preparation is prepared by mixing commercially available 99.9% pure melatonin and corn starch according to the mass ratio of 1:100, and the storage time of the preparation is not more than 7 days.
On the other hand, the invention also provides an application method of the compound preparation for maintaining the egg laying peak of the laying hens at the final stage of egg laying, which is realized according to the following steps:
(1) uniformly mixing commercially available melatonin with the purity of 99.9% and corn starch according to the mass ratio of 1:100 to prepare a compound preparation with the concentration of 10 mg/g; the storage time of the compound preparation prepared each time is not more than 7 days; the standard of the corn starch is above the first grade of the national standard (GB/T8885-2017);
(2) when the environmental temperature is lower than 35 ℃, adding the compound preparation prepared in the step (1) into the daily feed of the laying hens in a gradient adding mode according to one ten thousandth of the adding amount to prepare the melatonin-containing feed with the final melatonin concentration of 1 mg/kg; when the environmental temperature is higher than 35 ℃, adding the compound preparation prepared in the step (1) into the daily feed of laying hens in a gradient adding mode according to two ten-thousandth of adding amount to prepare the melatonin-containing feed with the final concentration of melatonin of 2 mg/kg; the storage time of the prepared feed containing the melatonin is not more than 3 days;
(3) feeding the melatonin-containing feed prepared in the step (2) when the laying rate of the laying hens at the final stage of laying is reduced to 85-92%; after the feed containing the melatonin is fed, the laying rate is not reduced, and the feed formula is not changed;
(4) after feeding the melatonin-containing feed for two weeks, stopping feeding the melatonin-containing feed for 1 week, and feeding the daily feed for the laying hens during the stopping period;
(5) stopping feeding the melatonin-containing feed for 1 week, and then feeding the melatonin-containing feed for 2 weeks;
(6) and (5) circularly feeding according to the feeding modes in the step (4) and the step (5) until the laying hens are rejected or the laying rate is reduced to be below 75%.
Wherein, the variety of the laying hens in the step (3) comprises but is not limited to Beijing white hens, Hailan brown laying hens, white Lai aviation laying hens, Roman brown shell laying hens and Jingbaifen shell laying hens.
(III) advantageous effects
The invention provides a compound preparation for maintaining the egg laying peak of laying hens in the final egg laying period and an application method. The compound preparation is prepared by uniformly mixing melatonin and corn starch, is added into the daily feed of laying hens in an exogenous manner, can effectively reduce the degradation of the melatonin, avoids the characteristic of poor water solubility of the melatonin, improves the utilization rate of the laying hens on the melatonin, is simple in operation method, and reduces the labor cost. When the environmental temperature is lower than 35 ℃, the final concentration of the melatonin addition is 1mg/kg, and when the environmental temperature is higher than 35 ℃, the final concentration of the melatonin addition is 2mg/kg, so that the egg laying peak period of the laying hens can be effectively prolonged and maintained for at least 12 weeks by the method, the egg breakage rate is reduced, the egg quality of eggs can be ensured not to be reduced, the waste caused by premature elimination of the laying hens is reduced, and the comprehensive breeding cost of the laying hens is reduced; the method can keep the primordial follicles and follicular granular cells of the laying hens in the final period of egg laying in regular shapes and has positive influence on the proliferation of the follicular cells.
Drawings
Fig. 1 is a transmission electron micrograph of follicular granulosa cells at week 12 of the experiment in the control and test groups, wherein fig. A, B, C, D is a transmission electron micrograph of follicular granulosa cells in the control group at different magnifications; FIG. E, F, G, H is a transmission electron micrograph of follicular granulosa cells of the experimental group at different magnifications.
FIG. 2 is a scan of tissue sections of primordial follicles at week 12 of the experiment for the control group and the experimental group, wherein A, B, C is a scan of tissue sections of primordial follicles at different magnifications for the control group; FIG. D, E, F is a scan of tissue sections from primordial follicles at different magnifications in the experimental group.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
A compound preparation for maintaining the egg laying peak of laying hens in the final egg laying period and an application method thereof are realized according to the following steps:
(1) uniformly mixing commercially available melatonin with the purity of 99.9% and corn starch according to the mass ratio of 1:100 to prepare a compound preparation with the concentration of 10 mg/g; the storage time of the compound preparation prepared each time is not more than 7 days; the standard of the corn starch is above the first grade of the national standard (GB/T8885-2017);
(2) when the environmental temperature is lower than 35 ℃, adding the compound preparation prepared in the step (1) into the daily feed of the laying hens in a gradient adding mode according to one ten thousandth of the adding amount to prepare the melatonin-containing feed with the final melatonin concentration of 1 mg/kg; when the environmental temperature is higher than 35 ℃, adding the compound preparation prepared in the step (1) into the daily feed of laying hens in a gradient adding mode according to two ten-thousandth of adding amount to prepare the melatonin-containing feed with the final concentration of melatonin of 2 mg/kg; the storage time of the prepared feed containing the melatonin is not more than 3 days;
(3) feeding the feed containing the melatonin prepared in the step (2) when the laying rate of the Hailan brown laying hens at the final stage of laying is reduced to 90.4%; after the feed containing melatonin is fed, the laying rate is not reduced, and the feed formula in the original laying peak period is still used;
(4) after feeding the melatonin-containing feed for two weeks, stopping feeding the melatonin-containing feed for 1 week, and feeding the daily feed for the laying hens during the stopping period;
(5) stopping feeding the melatonin-containing feed for 1 week, and then feeding the melatonin-containing feed for 2 weeks;
(6) and (5) circularly feeding according to the feeding modes in the step (4) and the step (5) until the laying hens are rejected or the laying rate is reduced to be below 75%.
Test example 1
To further illustrate the effect of different amounts of the complex formulation of the present invention on the maintenance of the peak production of the laying hens at the end of the laying period at different ambient temperatures, the following animal experiments are provided for illustration.
Test animals: 300 Hailan brown laying hens which have good physical condition and the laying rate of which is reduced to 90 percent are selected and averagely divided into two groups, namely a test group 1 and a test group 2, and 150 laying hens are selected.
Test protocol: 150 laying hens in the test group 1 are uniformly divided into 5 groups, namely a test group 1-1, a test group 1-2, a test group 1-3, a test group 1-4 and a test group 1-5; feeding the compound preparation and the feeding mode according to the embodiment 1, wherein the final concentration of the melatonin in each group is respectively 0mg/kg, 0.5mg/kg, 1mg/kg, 2mg/kg and 5 mg/kg;
150 laying hens in the test group 2 are uniformly divided into 5 groups, namely a test group 2-1, a test group 2-2, a test group 2-3, a test group 2-4 and a test group 2-5; feeding the compound preparation and the feeding mode according to the embodiment 1, wherein the final concentration of the melatonin in each group is respectively 0mg/kg, 0.5mg/kg, 1mg/kg, 2mg/kg and 5 mg/kg;
the feeding environment of the two groups is kept consistent during the test period, wherein the environment temperature of the test group 1 is lower than 35 ℃, the environment temperature of the test group 2 is higher than 35 ℃, free ingestion and free drinking are adopted, and the test time is 12 weeks.
Observation indexes are as follows: the laying rates of the hens of trial 1 and trial 2 before and at weeks 4, 8 and 12 of the trial were recorded and calculated.
Test results and analysis:
tables 1 and 2 show the influence of different concentrations of melatonin on the laying rate of the laying hens at the final stage of laying at different environmental temperatures of the test group 1 and the test group 2 respectively. Comparing the results in tables 1 and 2, it can be seen that the environmental temperature has a significant effect on the laying rate of the laying hens at the same concentration of melatonin, and that the laying rate can be maintained at about 90% and maintained for at least 12 weeks at the final concentration of melatonin of 1mg/kg at the environmental temperature of less than 35 ℃, with a significant difference (p <0.05) compared with other concentrations; at ambient temperatures above 35 ℃ and final melatonin concentrations of 2mg/kg, the egg production rate can be maintained at around 90% and for at least 12 weeks with a significant difference (p <0.05) compared to other concentrations.
Table 1 test group 1 influence of different concentrations of melatonin on laying rate of hens at the final stage of laying (ambient temperature lower than 35 c)
Note: different letters in the same row data indicate significant differences (p < 0.05).
Table 2 influence of different concentrations of melatonin on laying rate of hens at final laying period (ambient temperature lower than 35 c)
Note: different letters in the same row data indicate significant differences (p < 0.05).
Test example 2
To further illustrate the effect of the complex formulation of the present invention on the maintenance of the peak production of laying hens at the terminal stage of laying, the following animal experiments are provided for illustration.
Test animals: 300 Hailan brown laying hens which have good physical condition and the laying rate of which is reduced to 90.4 percent are selected and averagely divided into two groups, namely a test group and a control group, and 150 laying hens are selected.
Test protocol: the test group is fed according to the compound preparation and the feeding mode in the example 1; the control group is normally fed with daily feed for laying hens; during the test period, the two groups of feeding environments are kept consistent, the environmental temperature is lower than 35 ℃, free food intake and free drinking are adopted, and the test time is 12 weeks.
Observation indexes are as follows: (1) recording and calculating the laying rate of the laying hens of the control group and the test group before the test and at the 1 st, 2 nd, 3 rd, 4 th, 6 th, 8 th, 10 th and 12 th weeks of the test; (2) recording and calculating the egg breakage rate of the laying hens of the control group and the test group before the test and at the 1 st, 2 nd, 3 rd, 4 th, 8 th and 12 th weeks of the test; (3) recording the egg closing quality data of the laying hens of the control group and the test group, such as the egg weight, the egg protein height, the Haugh unit, the eggshell strength, the eggshell thickness and the like of the eggs at the 12 th week of the test; (4) and observing the shapes of the primordial follicles and follicular granular cells of the laying hens of the test group and the control group by a transmission electron microscope.
Test results and analysis:
(1) influence of the compound preparation of the invention on laying rate of laying hens at the end stage of laying
As can be seen from the results in table 1, no melatonin was added to the feed of the control group during the test period, the laying rate of the group started to decrease from the 1 st week of the test, and gradually decreased with the passage of the test time, and the laying rate of the control group decreased to 72.3% at the 12 th week of the test, which was significantly different from the laying rate before the test; compared with a control group, the feed of the test group is added with 1mg/kg of melatonin, the laying rate of the group has no tendency of reducing in the test period, and the laying rate of the group is still maintained at 91.7% in the 12 th week of the test, and is equivalent to the laying rate before the test, and the difference is not significant; the test results can show that the compound preparation and the application method can obviously prolong the egg laying peak of the laying hens by at least 12 weeks.
TABLE 1 laying rates of the test and control groups during the test period
Note: different letters in the same row data indicate significant differences (p <0.05), as follows.
(2) Influence of the compound preparation on egg breaking rate of laying hens in the final egg laying period
As can be seen from the results in table 2, no melatonin was added to the feed of the control group during the test period, the egg breaking rate of the group decreased from the 1 st week of the test and gradually increased with the passage of the test time, and the egg breaking rate of the control group increased to 5.78% at the 12 th week of the test, which is significantly different from the egg breaking rate before the test; compared with a control group, the feed of the test group is added with 1mg/kg of melatonin, the egg breaking rate of the group has no tendency of reducing in the test period, and 1.57 percent of the egg breaking rate is still maintained at the 12 th week of the test and is slightly lower than the egg breaking rate before the test; the test results show that the compound preparation and the application method can remarkably prolong the laying peak of the laying hens by at least 12 weeks, ensure the quality of eggs and maintain a low egg breaking rate.
TABLE 2 egg breakage during the test period for the test group and the control group
(3) Influence of the compound preparation on egg quality of eggs laid by laying hens in the final stage of egg laying
Table 3 shows the egg quality index of the control group and the test group at the 12 th week of the test. As can be seen from the results in table 3, the egg weights of the two groups of the control group are not significantly different from those of the test group, but the protein height, the haugh unit, the eggshell strength and the eggshell thickness of the test group are significantly higher than those of the control group, and the differences are significant; the test results show that the compound preparation and the application method can remarkably prolong the egg laying peak of the laying hens by at least 12 weeks, ensure the quality of eggs, maintain a low egg breaking rate and keep the egg quality of the eggs from being reduced.
TABLE 3 egg quality index at week 12 of the test and control groups
(4) The influence of the compound preparation on the primordial follicle and the follicular granulosa cell shape of the laying hens in the terminal period of egg laying
As is clear from the results shown in fig. 1 and 2, the number of malformations of primordial follicles of laying hens at the terminal stage of laying of the control group increased, and the cell layer of follicular granules became thin; the primordial follicles and follicular granulosa cells of the test group were regular in shape, and the cell proliferation was significantly higher than that of the control group.
Reference documents:
[1]Lin T,Lee J E,Kang J W,et al.Melatonin supplementation during prolonged in vitro maturation improves the quality and development of poor-quality porcine oocytes via anti-oxidative and anti-apoptotic effects[J].Mol Reprod Dev,2018,85(8-9): 665-681.
[2] influence of wang qingka melatonin and cumulus cells on in vitro maturation and embryonic development of porcine oocytes [ D ] engineering university of north and river, 2019.
[3]Soto-Heras S,Roura M,CataláM G,et al.Beneficial effects of melatonin on in vitro embryo production from juvenile goat oocytes[J].Reprod Fertil Dev,2018,30(2): 253-261.
[4]Wang F,Tian X,Zhang L,et al.Melatonin promotes the in vitro development of pronuclear embryos and increases the efficiency of blastocyst implantation in murine[J]. J Pineal Res,2013,55(3):267-274.
[5] Haoergon, Huangchenxuan, Sun Haoyang, etc. influence of exogenous melatonin on laying hen production performance, serum index and melatonin content in body at the later stage of egg laying [ J ] animal nutrition report 2020,32(05): 2126-.
[6]Jia Y,Yang M,Zhu K,et al.Melatonin implantation improved the egg-laying rate and quality in hens past their peak egg-laying age[J].Sci Rep,2016,6:39799-39807.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (7)
1. The compound preparation for maintaining the egg laying peak of laying hens at the final stage of egg laying is characterized by being prepared by uniformly mixing commercially available 99.9% pure melatonin and corn starch according to the mass ratio of 1: 100.
2. The complex preparation for maintaining the egg laying peak of laying hens at the end stage of egg laying according to claim 1, wherein the complex preparation is stored for no more than 7 days after each preparation.
3. The method for applying the compound preparation for maintaining the egg laying peak of the laying hens at the end stage of egg laying according to claim 1, which is implemented according to the following steps:
(1) uniformly mixing commercially available melatonin with the purity of 99.9% and corn starch according to the mass ratio of 1:100 to prepare a compound preparation with the concentration of 10 mg/g; the storage time of the compound preparation prepared each time is not more than 7 days; the standard of the corn starch is above the first grade of the national standard (GB/T8885-2017);
(2) adding the compound preparation prepared in the step (1) into daily feed of laying hens in a gradient adding manner to prepare feed containing melatonin;
(3) feeding the melatonin-containing feed prepared in the step (2) when the laying rate of the laying hens at the final stage of laying is reduced to 85-92%; after the feed containing the melatonin is fed, the laying rate is not reduced, and the feed formula is not changed;
(4) after feeding the melatonin-containing feed for two weeks, stopping feeding the melatonin-containing feed for 1 week, and feeding the daily feed for the laying hens during the stopping period;
(5) stopping feeding the melatonin-containing feed for 1 week, and then feeding the melatonin-containing feed for 2 weeks;
(6) and (5) circularly feeding according to the feeding modes of the step (4) and the step (5).
4. The method for applying the compound preparation for maintaining the egg laying peak of the laying hens at the end stage of egg laying according to claim 3, wherein in the step (2), when the ambient temperature is lower than 35 ℃, the compound preparation prepared in the step (1) is added into the daily feed of the laying hens in a gradient adding mode according to the addition amount of one ten thousandth, so as to prepare the feed containing the melatonin, wherein the final concentration of the melatonin is 1 mg/kg; when the environmental temperature is higher than 35 ℃, the compound preparation prepared in the step (1) is added into the daily feed of the laying hens in a gradient adding mode according to two ten-thousandth of adding amount to prepare the melatonin-containing feed with the final concentration of melatonin of 2 mg/kg.
5. The use of the complex formulation for maintaining the egg production peak of laying hens at the end of egg production according to claim 3, wherein the melatonin-containing feed prepared in each time in the step (2) is stored for not more than 3 days.
6. The method for applying the compound preparation for maintaining the egg production peak of the laying hens at the end stage of egg production according to claim 3, wherein the varieties of the laying hens in the step (3) comprise but are not limited to Beijing white hens, Hailan brown laying hens, white leghorned laying hens, Roman brown shell laying hens and Jingbaifen shell laying hens.
7. The application method of the compound preparation for maintaining the egg laying peak of the laying hens at the end stage of egg laying according to claim 3, wherein the compound preparation is circularly fed in the step (6) until the laying hens are eliminated or the egg laying rate is reduced to be less than 75%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111456999.7A CN114052143A (en) | 2021-12-02 | 2021-12-02 | Compound preparation for maintaining egg laying peak of laying hens in final egg laying period and application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111456999.7A CN114052143A (en) | 2021-12-02 | 2021-12-02 | Compound preparation for maintaining egg laying peak of laying hens in final egg laying period and application method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114052143A true CN114052143A (en) | 2022-02-18 |
Family
ID=80228286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111456999.7A Pending CN114052143A (en) | 2021-12-02 | 2021-12-02 | Compound preparation for maintaining egg laying peak of laying hens in final egg laying period and application method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114052143A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115176756A (en) * | 2022-06-22 | 2022-10-14 | 东北农业大学 | Method for relieving egg laying performance reduction of cage-bred laying hens in hot summer |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5976580A (en) * | 1995-06-07 | 1999-11-02 | Novus International, Inc. | Nutrient formulation and process for enhancing the health, livability, cumulative weight gain or feed efficiency in poultry and other animals |
| JP2003047410A (en) * | 2001-08-01 | 2003-02-18 | Itochu Shiryo Kk | Fowl egg-laying modifier and fowl feed formulated with the resultant modifier |
| CN103768434A (en) * | 2014-01-17 | 2014-05-07 | 临沂大学 | Traditional Chinese medicine for enhancing laying rate of laying hens and preparation method thereof |
| US20140255331A1 (en) * | 2011-10-13 | 2014-09-11 | Ovivity Group, S.L. | Egg preparation with regenerating, analgesic and/or anti-inflammatory properties |
| WO2015048339A2 (en) * | 2013-09-25 | 2015-04-02 | Pronutria, Inc. | Compositions and formulations for non-human nutrition and methods of production and use thereof |
| CN104705253A (en) * | 2015-02-16 | 2015-06-17 | 中国农业大学 | Method for improving egg laying rate of egg layers |
| CN108029637A (en) * | 2018-01-31 | 2018-05-15 | 浙江大学 | A kind of method that Layer Production Performance is maintained under the conditions of cold stress |
| KR20200072247A (en) * | 2018-12-12 | 2020-06-22 | 충북대학교 산학협력단 | method of Improving embryo quality and implantation rate using melatonin |
| CN111450043A (en) * | 2020-04-22 | 2020-07-28 | 河南牧业经济学院 | Melatonin sustained-release implant for livestock as well as preparation method and application thereof |
-
2021
- 2021-12-02 CN CN202111456999.7A patent/CN114052143A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5976580A (en) * | 1995-06-07 | 1999-11-02 | Novus International, Inc. | Nutrient formulation and process for enhancing the health, livability, cumulative weight gain or feed efficiency in poultry and other animals |
| JP2003047410A (en) * | 2001-08-01 | 2003-02-18 | Itochu Shiryo Kk | Fowl egg-laying modifier and fowl feed formulated with the resultant modifier |
| US20140255331A1 (en) * | 2011-10-13 | 2014-09-11 | Ovivity Group, S.L. | Egg preparation with regenerating, analgesic and/or anti-inflammatory properties |
| WO2015048339A2 (en) * | 2013-09-25 | 2015-04-02 | Pronutria, Inc. | Compositions and formulations for non-human nutrition and methods of production and use thereof |
| CN103768434A (en) * | 2014-01-17 | 2014-05-07 | 临沂大学 | Traditional Chinese medicine for enhancing laying rate of laying hens and preparation method thereof |
| CN104705253A (en) * | 2015-02-16 | 2015-06-17 | 中国农业大学 | Method for improving egg laying rate of egg layers |
| CN108029637A (en) * | 2018-01-31 | 2018-05-15 | 浙江大学 | A kind of method that Layer Production Performance is maintained under the conditions of cold stress |
| KR20200072247A (en) * | 2018-12-12 | 2020-06-22 | 충북대학교 산학협력단 | method of Improving embryo quality and implantation rate using melatonin |
| CN111450043A (en) * | 2020-04-22 | 2020-07-28 | 河南牧业经济学院 | Melatonin sustained-release implant for livestock as well as preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 姬向波;赵冰洁;刘玉萍;张桂枝;刘太宇;: "褪黑素调节LPS诱导奶牛乳腺上皮细胞炎性因子的表达及其机制", 动物医学进展, no. 07 * |
| 张苗蕊: "褪黑素对蛋鸡产蛋性能的影响研究", CNKI硕士电子期刊, pages 119 * |
| 郝二英;黄晨轩;孙浩政;王德贺;周荣艳;黄仁录;陈辉;: "外源褪黑素对产蛋后期蛋鸡生产性能、血清指标及体内褪黑素含量的影响", 动物营养学报, no. 05 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115176756A (en) * | 2022-06-22 | 2022-10-14 | 东北农业大学 | Method for relieving egg laying performance reduction of cage-bred laying hens in hot summer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Baldassarre et al. | Advanced assisted reproduction technologies (ART) in goats | |
| Abeydeera et al. | Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization | |
| JPH05508541A (en) | Enhanced implantation, proliferation and maintenance of embryos using leukemia inhibitory factors | |
| WO2016206086A1 (en) | Pig oocyte in vitro maturation culture solution and preparation method and culture method thereof | |
| CN104688380B (en) | Method for improving superovulating quantity and quality of sheep | |
| Heyman et al. | Zootechnical performance of cloned cattle and offspring: preliminary results | |
| CN1450857A (en) | Cryopreservation of sperm | |
| WO2019061560A1 (en) | In vitro maturation culture medium of immature oocytes and use thereof | |
| CN110312789A (en) | Composition and method for oocyte in vitro maturation | |
| Zhang et al. | Effects of melatonin on superovulation and transgenic embryo transplantation in small-tailed han sheep (Ovis aries) | |
| CN108142328B (en) | Early propagation and rapid seedling raising method for small yellow croakers | |
| CN114052143A (en) | Compound preparation for maintaining egg laying peak of laying hens in final egg laying period and application method | |
| US20120219940A1 (en) | Abalone sperm preservation method for the aquaculture industry | |
| CN113287547B (en) | Method for establishing full-male micropterus salmoides production system | |
| CN109897820B (en) | Method for delaying aging of in vitro cultured ovum | |
| Chang et al. | Factors affecting superovulation and embryo transfer in Boer goats | |
| CN1810967A (en) | Production process of somatic cell clone pig | |
| CN113661948A (en) | Method for obtaining full-female homozygous pelteobagrus fulvidraco clone line through self fertilization | |
| CN114287367A (en) | Cultivation method for artificially inducing finless eels to directly directionally develop into males | |
| CN114208815A (en) | Sperm diluent suitable for large-scale poultry farm and preparation method thereof | |
| CN107841484B (en) | In-vitro oocyte culture system and application thereof | |
| CN1226377A (en) | Process for industrializing technology of 'tube cattle' | |
| CN111034658A (en) | Artificial propagation method of acipenser schrenckii | |
| KR102183228B1 (en) | Composition for in vitro oocyte maturation and early embryonic development comprising Mito-TEMPO and culture method using the same | |
| RU2778147C1 (en) | Nutrient medium for the cultivation of bovine oocytes in vitro |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220218 |